AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48593


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48593.t1 AT1G72030 52.486 181 80 3 14 189 60 239 5.31e-53 170

Find 31 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAGTACCTTTACATTTC+AGG 0.191164 4.1:-66769630 MS.gene48593:CDS
TAGCTGTCTCCAATGCTTTC+AGG 0.230533 4.1:-66769605 MS.gene48593:intron
TTTGTTCTTTCAATTCTTTA+AGG 0.237732 4.1:-66770514 MS.gene48593:intron
TAGAGCTTATGAAGAAGATT+TGG 0.253716 4.1:-66769249 MS.gene48593:CDS
AAACTCATTGCCCCACAAAT+TGG 0.293774 4.1:+66769280 None:intergenic
TATATATAGGTATGCTTGTT+TGG 0.294474 4.1:-66769754 MS.gene48593:intron
AAATAGGCTGAAGTGCTTTC+AGG 0.312360 4.1:-66769986 MS.gene48593:intron
GTGAATATGGATGGAGAATA+AGG 0.363826 4.1:-66770623 MS.gene48593:CDS
AATCTTCTTCATAAGCTCTA+AGG 0.373693 4.1:+66769252 None:intergenic
TGAAAATATGAAGCAAAAGT+TGG 0.383459 4.1:-66770658 MS.gene48593:CDS
AGAGCTTATGAAGAAGATTT+GGG 0.390390 4.1:-66769248 MS.gene48593:CDS
TAACAACAAATGTACCTATC+AGG 0.398497 4.1:+66769935 None:intergenic
AGGTACTCTTGTGCTTCAGC+AGG 0.452395 4.1:+66769642 None:intergenic
GATTCATCAAAGGAACTTGT+AGG 0.457006 4.1:-66769714 MS.gene48593:CDS
TTAAATACTCACCTCCTGAT+AGG 0.463612 4.1:-66769949 MS.gene48593:intron
GTGACATAATATCCAATTTG+TGG 0.474667 4.1:-66769292 MS.gene48593:CDS
TGACATAATATCCAATTTGT+GGG 0.486196 4.1:-66769291 MS.gene48593:CDS
AAATCATTGAAAAGAGCTAC+AGG 0.509660 4.1:+66770534 None:intergenic
CAGCTATTCCTGAAATGTAA+AGG 0.520945 4.1:+66769622 None:intergenic
GGAGATGTTCAAGCACATCT+TGG 0.525750 4.1:+66769664 None:intergenic
ATATAGGTATGCTTGTTTGG+TGG 0.525798 4.1:-66769751 MS.gene48593:intron
AGAACATTGTACACAAATGC+TGG 0.538265 4.1:-66769221 MS.gene48593:CDS
GTGGATCTTTAGAAACAAGT+TGG 0.539203 4.1:+66769195 None:intergenic
CCAACCCAATTACTTGTCCA+TGG 0.539516 4.1:+66769173 None:intergenic
CATGAGAGAAGTGCGATCTG+AGG 0.595779 4.1:-66768481 MS.gene48593:CDS
GGTACTCTTGTGCTTCAGCA+GGG 0.601704 4.1:+66769643 None:intergenic
GAATGATCAAGATTCATCAA+AGG 0.633438 4.1:-66769724 MS.gene48593:CDS
ACCCAATTACTTGTCCATGG+TGG 0.661718 4.1:+66769176 None:intergenic
GACATAATATCCAATTTGTG+GGG 0.672118 4.1:-66769290 MS.gene48593:CDS
TTGTTTCTAAAGATCCACCA+TGG 0.675137 4.1:-66769190 MS.gene48593:CDS
CAACAAATGTACCTATCAGG+AGG 0.675327 4.1:+66769938 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAAAATAATAATTATTTT+AGG + chr4.1:66769295-66769314 None:intergenic 0.0%
!! TATATTATGCTATTATATAT+AGG - chr4.1:66769387-66769406 MS.gene48593:intron 10.0%
!!! ATATTCAAGTATTTTTAAAT+AGG - chr4.1:66769607-66769626 MS.gene48593:CDS 10.0%
!!! GTCAAAATTTATTATTTTAA+AGG + chr4.1:66769081-66769100 None:intergenic 10.0%
!! AATTAATAATCTACTTGATT+AGG + chr4.1:66770050-66770069 None:intergenic 15.0%
!! ATCATTTATCAATATGAAAT+TGG - chr4.1:66769266-66769285 MS.gene48593:CDS 15.0%
!! TAAAGACAAAATATTAGAAA+TGG - chr4.1:66770089-66770108 MS.gene48593:intron 15.0%
!! AACATTAAACAACCATTTAA+AGG + chr4.1:66769131-66769150 None:intergenic 20.0%
!! ATTTAAAGGCAAATAAAATG+TGG + chr4.1:66769117-66769136 None:intergenic 20.0%
!! TCTAAAGAATATAAAACACA+TGG - chr4.1:66770531-66770550 MS.gene48593:CDS 20.0%
!! TGTATTATATATGTGAAAGT+CGG + chr4.1:66768931-66768950 None:intergenic 20.0%
!! TTTGTTCTTTCAATTCTTTA+AGG - chr4.1:66768640-66768659 MS.gene48593:intron 20.0%
!!! ACATTTTATTTGCCTTTAAA+TGG - chr4.1:66769116-66769135 MS.gene48593:intron 20.0%
!!! CTATGTTTTGAATTTTTTTC+TGG + chr4.1:66770421-66770440 None:intergenic 20.0%
!!! TTATATTCTTTAGAAGCATA+TGG + chr4.1:66770525-66770544 None:intergenic 20.0%
! ATGTTAATGAGACTAATTGA+TGG - chr4.1:66769799-66769818 MS.gene48593:intron 25.0%
! CAATTTATTATCTCATGCTA+AGG + chr4.1:66769340-66769359 None:intergenic 25.0%
! TATATATAGGTATGCTTGTT+TGG - chr4.1:66769400-66769419 MS.gene48593:intron 25.0%
! TGAAAATATGAAGCAAAAGT+TGG - chr4.1:66768496-66768515 MS.gene48593:intron 25.0%
! TGACATAATATCCAATTTGT+GGG - chr4.1:66769863-66769882 MS.gene48593:intron 25.0%
! TTCTCTACTTATTGTCTTAA+AGG + chr4.1:66768893-66768912 None:intergenic 25.0%
!! AATAGTAACAAGATTTTGAG+TGG - chr4.1:66768753-66768772 MS.gene48593:intron 25.0%
!!! TATATGCTTCATGTTTTGAA+TGG + chr4.1:66770397-66770416 None:intergenic 25.0%
!!! TGTTCAAATCTTTTTGAAGA+CGG - chr4.1:66770302-66770321 MS.gene48593:intron 25.0%
AAAGGCAAATAAAATGTGGT+AGG + chr4.1:66769113-66769132 None:intergenic 30.0%
AAATCATTGAAAAGAGCTAC+AGG + chr4.1:66768623-66768642 None:intergenic 30.0%
AAGAAAATGTCGTGTTCTTA+TGG - chr4.1:66770004-66770023 MS.gene48593:intron 30.0%
AAGACAATAAGTAGAGAATC+TGG - chr4.1:66768895-66768914 MS.gene48593:intron 30.0%
AATCTTCTTCATAAGCTCTA+AGG + chr4.1:66769905-66769924 None:intergenic 30.0%
AGAGCTTATGAAGAAGATTT+GGG - chr4.1:66769906-66769925 MS.gene48593:intron 30.0%
ATGGTTCTTGCTACAAAATT+AGG + chr4.1:66770378-66770397 None:intergenic 30.0%
ATTCTAACCACTAGACAATT+TGG + chr4.1:66768866-66768885 None:intergenic 30.0%
ATTTCACCATAAAGAGAGTT+TGG + chr4.1:66768804-66768823 None:intergenic 30.0%
GAAATTGCTTCTCAAATTGT+AGG - chr4.1:66768819-66768838 MS.gene48593:intron 30.0%
GAATGATCAAGATTCATCAA+AGG - chr4.1:66769430-66769449 MS.gene48593:intron 30.0%
GAATGCAGATGAGATAAAAA+TGG - chr4.1:66768565-66768584 MS.gene48593:intron 30.0%
GACATAATATCCAATTTGTG+GGG - chr4.1:66769864-66769883 MS.gene48593:intron 30.0%
GTGACATAATATCCAATTTG+TGG - chr4.1:66769862-66769881 MS.gene48593:intron 30.0%
TAACAACAAATGTACCTATC+AGG + chr4.1:66769222-66769241 None:intergenic 30.0%
TAGAGCTTATGAAGAAGATT+TGG - chr4.1:66769905-66769924 MS.gene48593:intron 30.0%
TTCTAACCACTAGACAATTT+GGG + chr4.1:66768865-66768884 None:intergenic 30.0%
TTTGATCCAAACTCTCTTTA+TGG - chr4.1:66768795-66768814 MS.gene48593:intron 30.0%
! AGTTTTCTTTTTGTGTGTTC+AGG + chr4.1:66769663-66769682 None:intergenic 30.0%
! GTTTTCTTTTTGTGTGTTCA+GGG + chr4.1:66769662-66769681 None:intergenic 30.0%
!! TTTTAATCACCTGAAAGCAT+TGG + chr4.1:66769561-66769580 None:intergenic 30.0%
!! TTTTGAAGACGGTAGTAAAA+TGG - chr4.1:66770313-66770332 MS.gene48593:intron 30.0%
!!! GAAGCAAAAGTTGGATTTTT+TGG - chr4.1:66768505-66768524 MS.gene48593:intron 30.0%
!!! GATTTTTTGGTGTGTGAATA+TGG - chr4.1:66768518-66768537 MS.gene48593:intron 30.0%
AAAATGTGGTAGGTGCTTAA+TGG + chr4.1:66769103-66769122 None:intergenic 35.0%
AGAACATTGTACACAAATGC+TGG - chr4.1:66769933-66769952 MS.gene48593:intron 35.0%
ATATAGGTATGCTTGTTTGG+TGG - chr4.1:66769403-66769422 MS.gene48593:intron 35.0%
CAGCTATTCCTGAAATGTAA+AGG + chr4.1:66769535-66769554 None:intergenic 35.0%
CTGAACATAGCTCAGTTAAT+AGG + chr4.1:66768958-66768977 None:intergenic 35.0%
GATTCATCAAAGGAACTTGT+AGG - chr4.1:66769440-66769459 MS.gene48593:intron 35.0%
GTGAATATGGATGGAGAATA+AGG - chr4.1:66768531-66768550 MS.gene48593:intron 35.0%
TATCCATTCTTGACCCAAAT+AGG - chr4.1:66769152-66769171 MS.gene48593:intron 35.0%
TATTTCAGTGTCATCGATCA+TGG - chr4.1:66770260-66770279 MS.gene48593:intron 35.0%
TTAAATACTCACCTCCTGAT+AGG - chr4.1:66769205-66769224 MS.gene48593:CDS 35.0%
TTGCTTCTCAAATTGTAGGA+TGG - chr4.1:66768823-66768842 MS.gene48593:intron 35.0%
! GTGGATCTTTAGAAACAAGT+TGG + chr4.1:66769962-66769981 None:intergenic 35.0%
! TAAGTAGAGAATCTGGAGTT+CGG - chr4.1:66768902-66768921 MS.gene48593:intron 35.0%
! TCTTTTCCCAAATTGTCTAG+TGG - chr4.1:66768856-66768875 MS.gene48593:intron 35.0%
!! CAAGAGTACCTTTACATTTC+AGG - chr4.1:66769524-66769543 MS.gene48593:intron 35.0%
!! GATTAGTCTGCTTATGTAGA+AGG - chr4.1:66770647-66770666 MS.gene48593:CDS 35.0%
!! TTGTTTCTAAAGATCCACCA+TGG - chr4.1:66769964-66769983 MS.gene48593:CDS 35.0%
!! TTTTGGTGTGTGAATATGGA+TGG - chr4.1:66768522-66768541 MS.gene48593:intron 35.0%
AAACTCATTGCCCCACAAAT+TGG + chr4.1:66769877-66769896 None:intergenic 40.0%
ACATGGCACTACAACAGAAA+TGG - chr4.1:66770548-66770567 MS.gene48593:CDS 40.0%
AGAAGGAAAAGACGATTGCA+AGG + chr4.1:66770601-66770620 None:intergenic 40.0%
ATCCACCATGGACAAGTAAT+TGG - chr4.1:66769976-66769995 MS.gene48593:CDS 40.0%
CAACAAATGTACCTATCAGG+AGG + chr4.1:66769219-66769238 None:intergenic 40.0%
TCCACCATGGACAAGTAATT+GGG - chr4.1:66769977-66769996 MS.gene48593:CDS 40.0%
TCGCTAACATTAGCAACTAG+TGG - chr4.1:66769725-66769744 MS.gene48593:CDS 40.0%
! AAATAGGCTGAAGTGCTTTC+AGG - chr4.1:66769168-66769187 MS.gene48593:intron 40.0%
! ATGAGACTAATTGATGGCAC+AGG - chr4.1:66769805-66769824 MS.gene48593:intron 40.0%
! GTCGAACTAGATTTTGCAGA+AGG + chr4.1:66770618-66770637 None:intergenic 40.0%
!! GAAAGCACTTCAGCCTATTT+GGG + chr4.1:66769168-66769187 None:intergenic 40.0%
!! TGAAAGCACTTCAGCCTATT+TGG + chr4.1:66769169-66769188 None:intergenic 40.0%
ACCCAATTACTTGTCCATGG+TGG + chr4.1:66769981-66770000 None:intergenic 45.0%
CAGCCTATTTGGGTCAAGAA+TGG + chr4.1:66769158-66769177 None:intergenic 45.0%
CCAACCCAATTACTTGTCCA+TGG + chr4.1:66769984-66770003 None:intergenic 45.0%
CCATGGACAAGTAATTGGGT+TGG - chr4.1:66769981-66770000 MS.gene48593:CDS 45.0%
GGAGATGTTCAAGCACATCT+TGG + chr4.1:66769493-66769512 None:intergenic 45.0%
TAGCTGTCTCCAATGCTTTC+AGG - chr4.1:66769549-66769568 MS.gene48593:intron 45.0%
! AGACTAATTGATGGCACAGG+AGG - chr4.1:66769808-66769827 MS.gene48593:intron 45.0%
AGGTACTCTTGTGCTTCAGC+AGG + chr4.1:66769515-66769534 None:intergenic 50.0%
GGTACTCTTGTGCTTCAGCA+GGG + chr4.1:66769514-66769533 None:intergenic 50.0%
! CATGAGAGAAGTGCGATCTG+AGG - chr4.1:66770673-66770692 MS.gene48593:CDS 50.0%
! CTAATTGATGGCACAGGAGG+AGG - chr4.1:66769811-66769830 MS.gene48593:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 66768473 66770703 66768473 ID=MS.gene48593
chr4.1 mRNA 66768473 66770703 66768473 ID=MS.gene48593.t1;Parent=MS.gene48593
chr4.1 exon 66770515 66770703 66770515 ID=MS.gene48593.t1.exon1;Parent=MS.gene48593.t1
chr4.1 CDS 66770515 66770703 66770515 ID=cds.MS.gene48593.t1;Parent=MS.gene48593.t1
chr4.1 exon 66769950 66770002 66769950 ID=MS.gene48593.t1.exon2;Parent=MS.gene48593.t1
chr4.1 CDS 66769950 66770002 66769950 ID=cds.MS.gene48593.t1;Parent=MS.gene48593.t1
chr4.1 exon 66769606 66769767 66769606 ID=MS.gene48593.t1.exon3;Parent=MS.gene48593.t1
chr4.1 CDS 66769606 66769767 66769606 ID=cds.MS.gene48593.t1;Parent=MS.gene48593.t1
chr4.1 exon 66769186 66769349 66769186 ID=MS.gene48593.t1.exon4;Parent=MS.gene48593.t1
chr4.1 CDS 66769186 66769349 66769186 ID=cds.MS.gene48593.t1;Parent=MS.gene48593.t1
chr4.1 exon 66768473 66768510 66768473 ID=MS.gene48593.t1.exon5;Parent=MS.gene48593.t1
chr4.1 CDS 66768473 66768510 66768473 ID=cds.MS.gene48593.t1;Parent=MS.gene48593.t1
Gene Sequence

>MS.gene48593

ATGTTGGAAATTGAGAATGATAGTGAAAATATGAAGCAAAAGTTGGATTTTTTGGTGTGTGAATATGGATGGAGAATAAGGAGATTAATTGAGAATGCAGATGAGATAAAAATGGCTGCTCAAGTTCAAGCTGAAGCTTTTCATGATCCTGTAGCTCTTTTCAATGATTTGTTCTTTCAATTCTTTAAGGTTTTGTTTCTCTCTTTTTATTAGTGTTTATATTTTATCTTCATTTTTATGTACTTTTCATATGCTATTTTATTTCTTAAGATTTTATTTAAATAGTAACAAGATTTTGAGTGGAACTTGAGTGTAAAAACTGTTTGATCCAAACTCTCTTTATGGTGAAATTGCTTCTCAAATTGTAGGATGGTAAATTTAATTCTTTTCCCAAATTGTCTAGTGGTTAGAATTTCTCCTTTAAGACAATAAGTAGAGAATCTGGAGTTCGGACTCCGACTTTCACATATATAATACAATGTCCTATTAACTGAGCTATGTTCAGCGAGACAAATTTAGTCTCTTTAGATATAAGAAATTTTAAAATAATTTTTAAAAATTGAAAATGATTAATCTAATAATACTTTTAGATTAGTCAAATTAGTCCTTTAAAATAATAAATTTTGACCATTAAGCACCTACCACATTTTATTTGCCTTTAAATGGTTGTTTAATGTTTTATCCATTCTTGACCCAAATAGGCTGAAGTGCTTTCAGGACTGCTTTACAAGCTTAAATACTCACCTCCTGATAGGTACATTTGTTGTTATTTCTAAACAAGTTTATGTAGATTATCATTTATCAATATGAAATTGGTGACCTAAAATAATTATTATTTTTAATTAAAAAAAAACTCATGATAAACCTTAGCATGAGATAATAAATTGATCTTGTGTAAACATTAGCAACTAATGTATATTATGCTATTATATATAGGTATGCTTGTTTGGTGGCTGAGAATGATCAAGATTCATCAAAGGAACTTGTAGGTGTTATAGATGTTACTGTAATGAGAGACCAAGATGTGCTTGAACATCTCCCTGCTGAAGCACAAGAGTACCTTTACATTTCAGGAATAGCTGTCTCCAATGCTTTCAGGTGATTAAAAGATTTAATTACTATTACATCTCTGAAATATTCAAGTATTTTTAAATAGGTCATTGAAATAAAATATTTGTAACTTAGTCCCTGAACACACAAAAAGAAAACTGTTATATAGCGACTGATATTAGATATCAAAATATTAAATCGATCGCTAACATTAGCAACTAGTGGTTTAACGACATTTTACACATTGATATTTTTCATGTGATGTTGTGAAACTATATGTTAATGAGACTAATTGATGGCACAGGAGGAGGAAAATAGCAACTGTTATGTTGAAAGCATGTGACATAATATCCAATTTGTGGGGCAATGAGTTTCTTGTCCTTAGAGCTTATGAAGAAGATTTGGGTGCTAGAACATTGTACACAAATGCTGGCTACCAACTTGTTTCTAAAGATCCACCATGGACAAGTAATTGGGTTGGAAGAAAATGTCGTGTTCTTATGGTCAAAAGAATTAGTTTATTTCCTAATCAAGTAGATTATTAATTCAAAAATTTAAGATATATATAAAGACAAAATATTAGAAATGGCAAATGTATTATTAATTCAAAAATTAAGATATATATAAAGACAAAATATTAGAAATGGCAAATGTATTCTATGTATATAGCATATGTCAGCATATTTAGTTCATTATTATTTCTCATTGATTACACTCAACACAAGTTTCTCAAATACTATTTCAGTGTCATCGATCATGGATAGCGAAAAATAGCATTTTGTTCAAATCTTTTTGAAGACGGTAGTAAAATGGTCATTAGTGATACTTTAAGTAATGATTATTTTCTTGAATCCTAATTTTGTAGCAAGAACCATTCAAAACATGAAGCATATAACCAGAAAAAAATTCAAAACATAGCTGTAGTAGTTATTATGTTCACTTCTGAACTTCTAAGTGTTTTATGAAGCAAGCATATGTGAAGCAACTAAAATTCAGATTCCATATGCTTCTAAAGAATATAAAACACATGGCACTACAACAGAAATGGATTGCACTGAAAGTCAATGACACGATACCTTGCAATCGTCTTTTCCTTCTGCAAAATCTAGTTCGACATCTGCTTTGATTAGTCTGCTTATGTAGAAGGCGACATGAGAGAAGTGCGATCTGAGGTCGATTGA

Protein sequence

>MS.gene48593.t1

MLEIENDSENMKQKLDFLVCEYGWRIRRLIENADEIKMAAQVQAEAFHDPVALFNDLFFQFFKAEVLSGLLYKLKYSPPDRYACLVAENDQDSSKELVGVIDVTVMRDQDVLEHLPAEAQEYLYISGIAVSNAFRRRKIATVMLKACDIISNLWGNEFLVLRAYEEDLGARTLYTNAGYQLVSKDPPWTKGDMREVRSEVD