Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08832.t1 | XP_003629627.1 | 47 | 264 | 5 | 2 | 1 | 130 | 210 | 472 | 8.00E-47 | 196.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08832.t1 | K4CWS6 | 62.9 | 70 | 24 | 1 | 46 | 115 | 396 | 463 | 6.7e-18 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08832.t1 | G7LH44 | 47.0 | 264 | 5 | 2 | 1 | 130 | 210 | 472 | 5.8e-47 | 196.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene08832 | 0.804797 | 1.82E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene08832 | 0.82808 | 1.10E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene08832 | 0.832258 | 1.05E-55 | -1.69E-46 |
| MS.gene049232 | MS.gene08832 | 0.833559 | 4.97E-56 | -1.69E-46 |
| MS.gene049285 | MS.gene08832 | 0.812842 | 3.46E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene08832 | 0.825309 | 5.03E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene08832 | 0.857757 | 1.31E-62 | -1.69E-46 |
| MS.gene049369 | MS.gene08832 | 0.847068 | 1.46E-59 | -1.69E-46 |
| MS.gene049396 | MS.gene08832 | 0.875027 | 4.26E-68 | -1.69E-46 |
| MS.gene049609 | MS.gene08832 | 0.832718 | 8.06E-56 | -1.69E-46 |
| MS.gene049633 | MS.gene08832 | 0.811609 | 6.43E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene08832 | 0.839073 | 1.97E-57 | -1.69E-46 |
| MS.gene049907 | MS.gene08832 | 0.815188 | 1.05E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene08832 | 0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene050122 | MS.gene08832 | 0.816892 | 4.38E-52 | -1.69E-46 |
| MS.gene050216 | MS.gene08832 | 0.823789 | 1.15E-53 | -1.69E-46 |
| MS.gene050323 | MS.gene08832 | 0.820423 | 6.91E-53 | -1.69E-46 |
| MS.gene050380 | MS.gene08832 | -0.838488 | 2.79E-57 | -1.69E-46 |
| MS.gene050381 | MS.gene08832 | -0.810337 | 1.21E-50 | -1.69E-46 |
| MS.gene050499 | MS.gene08832 | 0.827569 | 1.45E-54 | -1.69E-46 |
| MS.gene050502 | MS.gene08832 | 0.842344 | 2.74E-58 | -1.69E-46 |
| MS.gene050504 | MS.gene08832 | 0.82253 | 2.26E-53 | -1.69E-46 |
| MS.gene050518 | MS.gene08832 | 0.810524 | 1.10E-50 | -1.69E-46 |
| MS.gene050520 | MS.gene08832 | 0.842518 | 2.46E-58 | -1.69E-46 |
| MS.gene050538 | MS.gene08832 | 0.809135 | 2.20E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene08832 | 0.806636 | 7.47E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene08832 | 0.820982 | 5.14E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene08832 | 0.826712 | 2.33E-54 | -1.69E-46 |
| MS.gene050662 | MS.gene08832 | 0.830293 | 3.19E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene08832 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene08832 | 0.83042 | 2.97E-55 | -1.69E-46 |
| MS.gene050686 | MS.gene08832 | 0.844944 | 5.52E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene08832 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene050747 | MS.gene08832 | 0.832685 | 8.21E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene08832 | 0.852845 | 3.53E-61 | -1.69E-46 |
| MS.gene05080 | MS.gene08832 | 0.855318 | 6.84E-62 | -1.69E-46 |
| MS.gene050868 | MS.gene08832 | 0.819557 | 1.09E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene08832 | 0.82262 | 2.15E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene08832 | 0.81915 | 1.35E-52 | -1.69E-46 |
| MS.gene050986 | MS.gene08832 | 0.812957 | 3.26E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene08832 | 0.828961 | 6.72E-55 | -1.69E-46 |
| MS.gene051387 | MS.gene08832 | 0.82158 | 3.74E-53 | -1.69E-46 |
| MS.gene051509 | MS.gene08832 | 0.863003 | 3.39E-64 | -1.69E-46 |
| MS.gene051666 | MS.gene08832 | 0.832844 | 7.50E-56 | -1.69E-46 |
| MS.gene051692 | MS.gene08832 | 0.822019 | 2.96E-53 | -1.69E-46 |
| MS.gene051734 | MS.gene08832 | 0.813634 | 2.32E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene08832 | 0.81495 | 1.19E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene08832 | 0.838577 | 2.65E-57 | -1.69E-46 |
| MS.gene052164 | MS.gene08832 | 0.84195 | 3.48E-58 | -1.69E-46 |
| MS.gene052346 | MS.gene08832 | 0.804071 | 2.58E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene08832 | 0.838245 | 3.22E-57 | -1.69E-46 |
| MS.gene052421 | MS.gene08832 | 0.812131 | 4.95E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene08832 | 0.821238 | 4.49E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene08832 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene052486 | MS.gene08832 | 0.808671 | 2.76E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene08832 | 0.814834 | 1.26E-51 | -1.69E-46 |
| MS.gene052506 | MS.gene08832 | 0.817836 | 2.68E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene08832 | 0.839473 | 1.55E-57 | -1.69E-46 |
| MS.gene052822 | MS.gene08832 | 0.815856 | 7.46E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene08832 | 0.80551 | 1.29E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene08832 | 0.829356 | 5.39E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene08832 | 0.828997 | 6.58E-55 | -1.69E-46 |
| MS.gene053018 | MS.gene08832 | 0.82795 | 1.18E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene08832 | 0.831549 | 1.57E-55 | -1.69E-46 |
| MS.gene053203 | MS.gene08832 | 0.810618 | 1.05E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene08832 | 0.832336 | 1.00E-55 | -1.69E-46 |
| MS.gene053213 | MS.gene08832 | 0.827951 | 1.18E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene08832 | 0.82351 | 1.33E-53 | -1.69E-46 |
| MS.gene053436 | MS.gene08832 | 0.844348 | 7.99E-59 | -1.69E-46 |
| MS.gene053449 | MS.gene08832 | 0.84089 | 6.62E-58 | -1.69E-46 |
| MS.gene053505 | MS.gene08832 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene08832 | 0.867617 | 1.20E-65 | -1.69E-46 |
| MS.gene053626 | MS.gene08832 | 0.866582 | 2.57E-65 | -1.69E-46 |
| MS.gene053632 | MS.gene08832 | 0.843515 | 1.34E-58 | -1.69E-46 |
| MS.gene053813 | MS.gene08832 | 0.833918 | 4.05E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene08832 | 0.813236 | 2.84E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene08832 | 0.823589 | 1.28E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene08832 | 0.871105 | 8.80E-67 | -1.69E-46 |
| MS.gene054003 | MS.gene08832 | 0.818173 | 2.25E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene08832 | 0.854643 | 1.07E-61 | -1.69E-46 |
| MS.gene054600 | MS.gene08832 | 0.832677 | 8.25E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene08832 | 0.829993 | 3.77E-55 | -1.69E-46 |
| MS.gene054706 | MS.gene08832 | -0.804872 | 1.75E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene08832 | 0.80932 | 2.01E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene08832 | 0.825476 | 4.59E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene08832 | 0.800427 | 1.45E-48 | -1.69E-46 |
| MS.gene05505 | MS.gene08832 | 0.85098 | 1.20E-60 | -1.69E-46 |
| MS.gene055206 | MS.gene08832 | 0.820128 | 8.08E-53 | -1.69E-46 |
| MS.gene055224 | MS.gene08832 | 0.827054 | 1.93E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene08832 | 0.811377 | 7.22E-51 | -1.69E-46 |
| MS.gene055486 | MS.gene08832 | 0.810315 | 1.23E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene08832 | 0.835231 | 1.89E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene08832 | 0.838672 | 2.50E-57 | -1.69E-46 |
| MS.gene055647 | MS.gene08832 | 0.809946 | 1.47E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene08832 | 0.851833 | 6.86E-61 | -1.69E-46 |
| MS.gene055741 | MS.gene08832 | 0.801418 | 9.09E-49 | -1.69E-46 |
| MS.gene055750 | MS.gene08832 | 0.829363 | 5.37E-55 | -1.69E-46 |
| MS.gene055909 | MS.gene08832 | 0.838056 | 3.61E-57 | -1.69E-46 |
| MS.gene055911 | MS.gene08832 | 0.839062 | 1.98E-57 | -1.69E-46 |
| MS.gene05592 | MS.gene08832 | 0.835252 | 1.87E-56 | -1.69E-46 |
| MS.gene055941 | MS.gene08832 | 0.852768 | 3.72E-61 | -1.69E-46 |
| MS.gene056166 | MS.gene08832 | 0.842146 | 3.09E-58 | -1.69E-46 |
| MS.gene056374 | MS.gene08832 | 0.812065 | 5.11E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene08832 | 0.878022 | 3.93E-69 | -1.69E-46 |
| MS.gene056446 | MS.gene08832 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene056448 | MS.gene08832 | 0.83995 | 1.17E-57 | -1.69E-46 |
| MS.gene056487 | MS.gene08832 | 0.843883 | 1.07E-58 | -1.69E-46 |
| MS.gene056504 | MS.gene08832 | 0.830313 | 3.15E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene08832 | 0.819429 | 1.17E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene08832 | 0.846778 | 1.75E-59 | -1.69E-46 |
| MS.gene05664 | MS.gene08832 | 0.815614 | 8.45E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene08832 | 0.838029 | 3.67E-57 | -1.69E-46 |
| MS.gene05666 | MS.gene08832 | 0.819905 | 9.09E-53 | -1.69E-46 |
| MS.gene056942 | MS.gene08832 | 0.802622 | 5.14E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene08832 | 0.86212 | 6.35E-64 | -1.69E-46 |
| MS.gene057086 | MS.gene08832 | 0.876765 | 1.08E-68 | -1.69E-46 |
| MS.gene057289 | MS.gene08832 | 0.821388 | 4.15E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene08832 | 0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene08832 | 0.819544 | 1.10E-52 | -1.69E-46 |
| MS.gene057537 | MS.gene08832 | 0.816308 | 5.91E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene08832 | 0.859733 | 3.37E-63 | -1.69E-46 |
| MS.gene057749 | MS.gene08832 | 0.801396 | 9.18E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene08832 | 0.818672 | 1.74E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene08832 | 0.828634 | 8.06E-55 | -1.69E-46 |
| MS.gene05787 | MS.gene08832 | 0.817365 | 3.42E-52 | -1.69E-46 |
| MS.gene057926 | MS.gene08832 | 0.845947 | 2.96E-59 | -1.69E-46 |
| MS.gene057927 | MS.gene08832 | 0.811664 | 6.25E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene08832 | 0.839036 | 2.01E-57 | -1.69E-46 |
| MS.gene058120 | MS.gene08832 | 0.810805 | 9.60E-51 | -1.69E-46 |
| MS.gene058130 | MS.gene08832 | 0.823777 | 1.15E-53 | -1.69E-46 |
| MS.gene058338 | MS.gene08832 | 0.804679 | 1.92E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene08832 | 0.821809 | 3.31E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene08832 | 0.833897 | 4.10E-56 | -1.69E-46 |
| MS.gene058459 | MS.gene08832 | 0.823064 | 1.69E-53 | -1.69E-46 |
| MS.gene058788 | MS.gene08832 | 0.807243 | 5.56E-50 | -1.69E-46 |
| MS.gene058790 | MS.gene08832 | 0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene058838 | MS.gene08832 | 0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene08832 | 0.80214 | 6.46E-49 | -1.69E-46 |
| MS.gene058948 | MS.gene08832 | 0.821354 | 4.22E-53 | -1.69E-46 |
| MS.gene059037 | MS.gene08832 | -0.814802 | 1.28E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene08832 | 0.843235 | 1.59E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene08832 | 0.8016 | 8.34E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene08832 | 0.888649 | 4.90E-73 | -1.69E-46 |
| MS.gene059367 | MS.gene08832 | 0.959319 | 2.60E-117 | -1.69E-46 |
| MS.gene059368 | MS.gene08832 | 0.964396 | 2.83E-123 | -1.69E-46 |
| MS.gene059455 | MS.gene08832 | 0.846443 | 2.17E-59 | -1.69E-46 |
| MS.gene059667 | MS.gene08832 | 0.819326 | 1.23E-52 | -1.69E-46 |
| MS.gene059674 | MS.gene08832 | 0.820271 | 7.49E-53 | -1.69E-46 |
| MS.gene059680 | MS.gene08832 | 0.822545 | 2.24E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene08832 | 0.85042 | 1.72E-60 | -1.69E-46 |
| MS.gene059718 | MS.gene08832 | 0.834004 | 3.85E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene08832 | 0.830567 | 2.73E-55 | -1.69E-46 |
| MS.gene060193 | MS.gene08832 | 0.804986 | 1.66E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene08832 | 0.800766 | 1.24E-48 | -1.69E-46 |
| MS.gene060482 | MS.gene08832 | 0.874062 | 9.05E-68 | -1.69E-46 |
| MS.gene060778 | MS.gene08832 | 0.848562 | 5.66E-60 | -1.69E-46 |
| MS.gene060804 | MS.gene08832 | 0.847338 | 1.23E-59 | -1.69E-46 |
| MS.gene060818 | MS.gene08832 | 0.826313 | 2.91E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene08832 | 0.884416 | 1.95E-71 | -1.69E-46 |
| MS.gene060837 | MS.gene08832 | 0.879633 | 1.06E-69 | -1.69E-46 |
| MS.gene06092 | MS.gene08832 | 0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene06097 | MS.gene08832 | 0.83339 | 5.48E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene08832 | 0.825981 | 3.48E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene08832 | 0.850111 | 2.10E-60 | -1.69E-46 |
| MS.gene061200 | MS.gene08832 | 0.815452 | 9.18E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAAGATGTGTGGAAGAT+AGG | 0.321918 | 4.2:-13432091 | MS.gene08832:intron |
| CGTAAACACCTTTGAAGCTT+TGG | 0.337494 | 4.2:-13432587 | MS.gene08832:CDS |
| AAGGTGTTTACGAGTATTAT+CGG | 0.367438 | 4.2:+13432598 | None:intergenic |
| GTGGATGAGGATGGAATTGT+AGG | 0.375063 | 4.2:-13432049 | MS.gene08832:CDS |
| GGATGAAATTAGAAAGTGTT+TGG | 0.378934 | 4.2:-13432023 | MS.gene08832:CDS |
| TGAGAAAGAATGCAATGAAA+TGG | 0.388985 | 4.2:-13431964 | MS.gene08832:CDS |
| GTGATGGGGAGAGGAGAGAA+AGG | 0.406369 | 4.2:-13431995 | MS.gene08832:CDS |
| AAGGGGTTGGCTAGAGAGGC+AGG | 0.409507 | 4.2:-13431941 | MS.gene08832:CDS |
| GTCCCGCAGAAAAGAATCTA+AGG | 0.412896 | 4.2:-13431907 | MS.gene08832:CDS |
| TTGAAGATGTGTGGAAGATA+GGG | 0.430114 | 4.2:-13432090 | MS.gene08832:intron |
| CTCTGGCTCCAAAGCTTCAA+AGG | 0.465787 | 4.2:+13432579 | None:intergenic |
| GCTAGAGAGGCAGGGAAGGA+AGG | 0.496328 | 4.2:-13431932 | MS.gene08832:CDS |
| AAAGTGTTTGGAAGTGGTGA+TGG | 0.499781 | 4.2:-13432011 | MS.gene08832:CDS |
| TTGGAAGTGGTGATGGGGAG+AGG | 0.502226 | 4.2:-13432004 | MS.gene08832:CDS |
| TCAACGGCTCTAAGTGCCTC+TGG | 0.506658 | 4.2:+13432562 | None:intergenic |
| GTTGGCTAGAGAGGCAGGGA+AGG | 0.508792 | 4.2:-13431936 | MS.gene08832:CDS |
| GGTGAGAGTTGATGATAAAG+TGG | 0.520496 | 4.2:-13432068 | MS.gene08832:CDS |
| GTACTACCGGAGGGAATCAA+TGG | 0.525946 | 4.2:+13432505 | None:intergenic |
| ATGGAAGGGGTTGGCTAGAG+AGG | 0.536943 | 4.2:-13431945 | MS.gene08832:CDS |
| ATCGGACCATTGATTCCCTC+CGG | 0.545735 | 4.2:-13432511 | MS.gene08832:intron |
| AAGTGTTTGGAAGTGGTGAT+GGG | 0.545845 | 4.2:-13432010 | MS.gene08832:CDS |
| GTTTACGAGTATTATCGGAT+TGG | 0.548691 | 4.2:+13432603 | None:intergenic |
| CTTTGAAGCTTTGGAGCCAG+AGG | 0.557170 | 4.2:-13432578 | MS.gene08832:CDS |
| AGTTGATGATAAAGTGGATG+AGG | 0.557996 | 4.2:-13432062 | MS.gene08832:CDS |
| GGAGAATGCATGCATGGATA+CGG | 0.559681 | 4.2:-13431861 | MS.gene08832:CDS |
| ATGAGGATGGAATTGTAGGA+GGG | 0.563817 | 4.2:-13432045 | MS.gene08832:CDS |
| AAAGAATGCAATGAAATGGA+AGG | 0.567197 | 4.2:-13431960 | MS.gene08832:CDS |
| AATTAGAAAGTGTTTGGAAG+TGG | 0.577533 | 4.2:-13432017 | MS.gene08832:CDS |
| TGCAATGAAATGGAAGGGGT+TGG | 0.578497 | 4.2:-13431954 | MS.gene08832:CDS |
| AAGAATGCAATGAAATGGAA+GGG | 0.580916 | 4.2:-13431959 | MS.gene08832:CDS |
| GGAGGGAATCAATGGTCCGA+TGG | 0.581754 | 4.2:+13432513 | None:intergenic |
| AGAGAGGCAGGGAAGGAAGG+TGG | 0.586569 | 4.2:-13431929 | MS.gene08832:CDS |
| GATGAGGATGGAATTGTAGG+AGG | 0.587770 | 4.2:-13432046 | MS.gene08832:CDS |
| TGAAGATGTGTGGAAGATAG+GGG | 0.590800 | 4.2:-13432089 | MS.gene08832:intron |
| GAGGTTATGATGAGTATCAA+CGG | 0.591472 | 4.2:+13432546 | None:intergenic |
| GAGGGAATCAATGGTCCGAT+GGG | 0.595436 | 4.2:+13432514 | None:intergenic |
| ATTATTGGAGAATGCATGCA+TGG | 0.597677 | 4.2:-13431867 | MS.gene08832:CDS |
| AGAATGCAATGAAATGGAAG+GGG | 0.608881 | 4.2:-13431958 | MS.gene08832:CDS |
| CAAAACTAATTGAAGATGTG+TGG | 0.610455 | 4.2:-13432099 | MS.gene08832:intron |
| AGGGGTTGGCTAGAGAGGCA+GGG | 0.613392 | 4.2:-13431940 | MS.gene08832:CDS |
| GGGGAGAGGAGAGAAAGGAG+AGG | 0.615489 | 4.2:-13431990 | MS.gene08832:CDS |
| GATGATAAAGTGGATGAGGA+TGG | 0.616208 | 4.2:-13432058 | MS.gene08832:CDS |
| TGAGGATGGAATTGTAGGAG+GGG | 0.617822 | 4.2:-13432044 | MS.gene08832:CDS |
| AACCTCAAAATGATTCCCAT+CGG | 0.625304 | 4.2:-13432529 | MS.gene08832:CDS |
| AGTGTTTGGAAGTGGTGATG+GGG | 0.640210 | 4.2:-13432009 | MS.gene08832:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGGATGATATATTAT+TGG | - | chr4.2:13432595-13432614 | MS.gene08832:CDS | 15.0% |
| !!! | AAAGAATCTAAGGAAATTTT+TGG | - | chr4.2:13432580-13432599 | MS.gene08832:CDS | 20.0% |
| ! | CATTCAATATAGTCATTTGA+AGG | + | chr4.2:13432023-13432042 | None:intergenic | 25.0% |
| ! | CTTCAAATGACTATATTGAA+TGG | - | chr4.2:13432021-13432040 | MS.gene08832:CDS | 25.0% |
| ! | GTTGAAATTTAAAGATGAGT+TGG | - | chr4.2:13432190-13432209 | MS.gene08832:intron | 25.0% |
| !! | ACATAAACAACAGAAGATTT+TGG | + | chr4.2:13432059-13432078 | None:intergenic | 25.0% |
| !!! | TCTGTTGTTTATGTTTCTTT+TGG | - | chr4.2:13432065-13432084 | MS.gene08832:CDS | 25.0% |
| AAAGAATGCAATGAAATGGA+AGG | - | chr4.2:13432517-13432536 | MS.gene08832:CDS | 30.0% | |
| AAGAATGCAATGAAATGGAA+GGG | - | chr4.2:13432518-13432537 | MS.gene08832:CDS | 30.0% | |
| CAAAACTAATTGAAGATGTG+TGG | - | chr4.2:13432378-13432397 | MS.gene08832:intron | 30.0% | |
| GTTCTTTCACATTCTTCTTT+GGG | - | chr4.2:13432254-13432273 | MS.gene08832:intron | 30.0% | |
| TGAGAAAGAATGCAATGAAA+TGG | - | chr4.2:13432513-13432532 | MS.gene08832:CDS | 30.0% | |
| ! | AAAGATGAGTTGGAAAAGAA+GGG | - | chr4.2:13432200-13432219 | MS.gene08832:intron | 30.0% |
| ! | AAGGTGTTTACGAGTATTAT+CGG | + | chr4.2:13431882-13431901 | None:intergenic | 30.0% |
| ! | TAAAGATGAGTTGGAAAAGA+AGG | - | chr4.2:13432199-13432218 | MS.gene08832:intron | 30.0% |
| ! | TTTCCTTAGATTCTTTTCTG+CGG | + | chr4.2:13432576-13432595 | None:intergenic | 30.0% |
| !! | AATTAGAAAGTGTTTGGAAG+TGG | - | chr4.2:13432460-13432479 | MS.gene08832:intron | 30.0% |
| !! | GGATGAAATTAGAAAGTGTT+TGG | - | chr4.2:13432454-13432473 | MS.gene08832:intron | 30.0% |
| AACCTCAAAATGATTCCCAT+CGG | - | chr4.2:13431948-13431967 | MS.gene08832:CDS | 35.0% | |
| AGAATGCAATGAAATGGAAG+GGG | - | chr4.2:13432519-13432538 | MS.gene08832:CDS | 35.0% | |
| AGTCATTTGAAGGTTGCAAT+AGG | + | chr4.2:13432013-13432032 | None:intergenic | 35.0% | |
| ATTATTGGAGAATGCATGCA+TGG | - | chr4.2:13432610-13432629 | MS.gene08832:CDS | 35.0% | |
| ATTGAAGATGTGTGGAAGAT+AGG | - | chr4.2:13432386-13432405 | MS.gene08832:intron | 35.0% | |
| GAGGTTATGATGAGTATCAA+CGG | + | chr4.2:13431934-13431953 | None:intergenic | 35.0% | |
| GGTTCTTTCACATTCTTCTT+TGG | - | chr4.2:13432253-13432272 | MS.gene08832:intron | 35.0% | |
| GTTTACGAGTATTATCGGAT+TGG | + | chr4.2:13431877-13431896 | None:intergenic | 35.0% | |
| TTGAAGATGTGTGGAAGATA+GGG | - | chr4.2:13432387-13432406 | MS.gene08832:intron | 35.0% | |
| ! | AAGATGAGTTGGAAAAGAAG+GGG | - | chr4.2:13432201-13432220 | MS.gene08832:intron | 35.0% |
| ! | ACTTTGGAAAGTTTGGTATC+GGG | - | chr4.2:13432308-13432327 | MS.gene08832:intron | 35.0% |
| ! | ATTGTGGGTTTTCATTCTTG+TGG | - | chr4.2:13432144-13432163 | MS.gene08832:intron | 35.0% |
| ! | GACTTTGGAAAGTTTGGTAT+CGG | - | chr4.2:13432307-13432326 | MS.gene08832:intron | 35.0% |
| ! | TTCCTTAGATTCTTTTCTGC+GGG | + | chr4.2:13432575-13432594 | None:intergenic | 35.0% |
| ! | TTGTGGGTTTTCATTCTTGT+GGG | - | chr4.2:13432145-13432164 | MS.gene08832:intron | 35.0% |
| !! | AGTTGATGATAAAGTGGATG+AGG | - | chr4.2:13432415-13432434 | MS.gene08832:intron | 35.0% |
| !!! | GGTTGTTTTTTGACTCATTG+TGG | - | chr4.2:13432275-13432294 | MS.gene08832:intron | 35.0% |
| !!! | GTTTTTTGACTCATTGTGGT+TGG | - | chr4.2:13432279-13432298 | MS.gene08832:intron | 35.0% |
| AAGTGTTTGGAAGTGGTGAT+GGG | - | chr4.2:13432467-13432486 | MS.gene08832:intron | 40.0% | |
| ATGAGGATGGAATTGTAGGA+GGG | - | chr4.2:13432432-13432451 | MS.gene08832:intron | 40.0% | |
| CAAGTGCGTTGTTGAATTGT+GGG | - | chr4.2:13432129-13432148 | MS.gene08832:intron | 40.0% | |
| GATGATAAAGTGGATGAGGA+TGG | - | chr4.2:13432419-13432438 | MS.gene08832:intron | 40.0% | |
| GGTCATGAGAGAAAAAGAAG+AGG | - | chr4.2:13432166-13432185 | MS.gene08832:intron | 40.0% | |
| TGAAGATGTGTGGAAGATAG+GGG | - | chr4.2:13432388-13432407 | MS.gene08832:intron | 40.0% | |
| ! | AAAGTGTTTGGAAGTGGTGA+TGG | - | chr4.2:13432466-13432485 | MS.gene08832:intron | 40.0% |
| ! | CTTTGGAAAGTTTGGTATCG+GGG | - | chr4.2:13432309-13432328 | MS.gene08832:intron | 40.0% |
| ! | GAACTCGACTTTGGAAAGTT+TGG | - | chr4.2:13432301-13432320 | MS.gene08832:intron | 40.0% |
| ! | GTCTTTTGATCAGTCCATTG+AGG | + | chr4.2:13432353-13432372 | None:intergenic | 40.0% |
| ! | TTTGGAAAGTTTGGTATCGG+GGG | - | chr4.2:13432310-13432329 | MS.gene08832:intron | 40.0% |
| !! | CGTAAACACCTTTGAAGCTT+TGG | - | chr4.2:13431890-13431909 | MS.gene08832:CDS | 40.0% |
| !! | GGTGAGAGTTGATGATAAAG+TGG | - | chr4.2:13432409-13432428 | MS.gene08832:intron | 40.0% |
| AGAAGGGGAAGATAGTGAAG+TGG | - | chr4.2:13432216-13432235 | MS.gene08832:intron | 45.0% | |
| AGTGAAGTGGTGTTCTCAAG+TGG | - | chr4.2:13432229-13432248 | MS.gene08832:intron | 45.0% | |
| CATTGAGGAAATGCCACTAG+AGG | + | chr4.2:13432338-13432357 | None:intergenic | 45.0% | |
| CTCTAGTGGCATTTCCTCAA+TGG | - | chr4.2:13432336-13432355 | MS.gene08832:intron | 45.0% | |
| GATGAGGATGGAATTGTAGG+AGG | - | chr4.2:13432431-13432450 | MS.gene08832:intron | 45.0% | |
| GCAAGTGCGTTGTTGAATTG+TGG | - | chr4.2:13432128-13432147 | MS.gene08832:intron | 45.0% | |
| GGAGAATGCATGCATGGATA+CGG | - | chr4.2:13432616-13432635 | MS.gene08832:CDS | 45.0% | |
| GTCCCGCAGAAAAGAATCTA+AGG | - | chr4.2:13432570-13432589 | MS.gene08832:CDS | 45.0% | |
| GTGGATGAGGATGGAATTGT+AGG | - | chr4.2:13432428-13432447 | MS.gene08832:intron | 45.0% | |
| TCCGGTAGTACTTCCTTTAG+CGG | - | chr4.2:13431984-13432003 | MS.gene08832:CDS | 45.0% | |
| TGAGGATGGAATTGTAGGAG+GGG | - | chr4.2:13432433-13432452 | MS.gene08832:intron | 45.0% | |
| TGCAATGAAATGGAAGGGGT+TGG | - | chr4.2:13432523-13432542 | MS.gene08832:CDS | 45.0% | |
| TTGCAATAGGTCACCGCTAA+AGG | + | chr4.2:13432000-13432019 | None:intergenic | 45.0% | |
| TTGTGGTTGGAACTCGACTT+TGG | - | chr4.2:13432292-13432311 | MS.gene08832:intron | 45.0% | |
| ! | AGTGTTTGGAAGTGGTGATG+GGG | - | chr4.2:13432468-13432487 | MS.gene08832:intron | 45.0% |
| ! | GTCCGATGGGAATCATTTTG+AGG | + | chr4.2:13431953-13431972 | None:intergenic | 45.0% |
| !! | ACCGCTAAAGGAAGTACTAC+CGG | + | chr4.2:13431988-13432007 | None:intergenic | 45.0% |
| !! | CTAAAGGAAGTACTACCGGA+GGG | + | chr4.2:13431984-13432003 | None:intergenic | 45.0% |
| GAAGTGGTGTTCTCAAGTGG+AGG | - | chr4.2:13432232-13432251 | MS.gene08832:intron | 50.0% | |
| GAGGGAATCAATGGTCCGAT+GGG | + | chr4.2:13431966-13431985 | None:intergenic | 50.0% | |
| GTACTACCGGAGGGAATCAA+TGG | + | chr4.2:13431975-13431994 | None:intergenic | 50.0% | |
| ! | CTCTGGCTCCAAAGCTTCAA+AGG | + | chr4.2:13431901-13431920 | None:intergenic | 50.0% |
| !! | ATCGGACCATTGATTCCCTC+CGG | - | chr4.2:13431966-13431985 | MS.gene08832:CDS | 50.0% |
| !! | CTTTGAAGCTTTGGAGCCAG+AGG | - | chr4.2:13431899-13431918 | MS.gene08832:CDS | 50.0% |
| !! | GCTAAAGGAAGTACTACCGG+AGG | + | chr4.2:13431985-13432004 | None:intergenic | 50.0% |
| GGAGGGAATCAATGGTCCGA+TGG | + | chr4.2:13431967-13431986 | None:intergenic | 55.0% | |
| ! | GTGATGGGGAGAGGAGAGAA+AGG | - | chr4.2:13432482-13432501 | MS.gene08832:intron | 55.0% |
| !! | ATGGAAGGGGTTGGCTAGAG+AGG | - | chr4.2:13432532-13432551 | MS.gene08832:CDS | 55.0% |
| !! | TCAACGGCTCTAAGTGCCTC+TGG | + | chr4.2:13431918-13431937 | None:intergenic | 55.0% |
| !! | TTGGAAGTGGTGATGGGGAG+AGG | - | chr4.2:13432473-13432492 | MS.gene08832:intron | 55.0% |
| AGAGAGGCAGGGAAGGAAGG+TGG | - | chr4.2:13432548-13432567 | MS.gene08832:CDS | 60.0% | |
| GCTAGAGAGGCAGGGAAGGA+AGG | - | chr4.2:13432545-13432564 | MS.gene08832:CDS | 60.0% | |
| GGGGAGAGGAGAGAAAGGAG+AGG | - | chr4.2:13432487-13432506 | MS.gene08832:intron | 60.0% | |
| GTTGGCTAGAGAGGCAGGGA+AGG | - | chr4.2:13432541-13432560 | MS.gene08832:CDS | 60.0% | |
| ! | GGTATCGGGGGTTCCTCTAG+TGG | - | chr4.2:13432322-13432341 | MS.gene08832:intron | 60.0% |
| !! | AAGGGGTTGGCTAGAGAGGC+AGG | - | chr4.2:13432536-13432555 | MS.gene08832:CDS | 60.0% |
| !! | AGGGGTTGGCTAGAGAGGCA+GGG | - | chr4.2:13432537-13432556 | MS.gene08832:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13431850 | 13432649 | 13431850 | ID=MS.gene08832 |
| chr4.2 | mRNA | 13431850 | 13432649 | 13431850 | ID=MS.gene08832.t1;Parent=MS.gene08832 |
| chr4.2 | exon | 13432512 | 13432649 | 13432512 | ID=MS.gene08832.t1.exon1;Parent=MS.gene08832.t1 |
| chr4.2 | CDS | 13432512 | 13432649 | 13432512 | ID=cds.MS.gene08832.t1;Parent=MS.gene08832.t1 |
| chr4.2 | exon | 13431850 | 13432106 | 13431850 | ID=MS.gene08832.t1.exon2;Parent=MS.gene08832.t1 |
| chr4.2 | CDS | 13431850 | 13432106 | 13431850 | ID=cds.MS.gene08832.t1;Parent=MS.gene08832.t1 |
| Gene Sequence |
| Protein sequence |
