AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58817


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58817.t1 MTR_2g036690 98.522 203 3 0 1 203 40 242 4.60e-144 401
MS.gene58817.t1 MTR_2g036690 97.872 141 3 0 1 141 40 180 1.74e-97 281
MS.gene58817.t1 MTR_2g005900 71.951 164 46 0 39 202 60 223 2.74e-78 234
MS.gene58817.t1 MTR_2g005900 40.217 92 50 2 113 200 53 143 3.25e-11 60.8
MS.gene58817.t1 MTR_7g109850 38.650 163 94 4 32 191 49 208 2.46e-25 98.2
MS.gene58817.t1 MTR_8g038785 44.318 88 49 0 31 118 48 135 3.14e-16 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58817.t1 AT4G25910 70.588 204 57 2 1 203 35 236 3.08e-95 277
MS.gene58817.t1 AT5G49940 70.787 178 48 1 25 202 62 235 2.26e-81 242
MS.gene58817.t1 AT5G49940 65.812 117 36 1 25 141 62 174 2.69e-44 146
MS.gene58817.t1 AT5G49940 47.297 74 34 2 133 202 85 157 6.35e-12 62.0
MS.gene58817.t1 AT4G01940 36.649 191 115 5 2 191 38 223 4.80e-26 100

Find 37 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAACCTGCTTTGTTTCT+TGG 0.155090 2.2:-50405601 None:intergenic
TGTTCAACCTCCAAGATTTC+TGG 0.161640 2.2:-50406292 None:intergenic
GAAATTAGACCATATCTTAT+TGG 0.195631 2.2:+50406724 MS.gene58817:CDS
AGAGAATGTAGAGAAAGTTT+TGG 0.294107 2.2:+50406108 MS.gene58817:CDS
AGACCATATCTTATTGGAAC+TGG 0.334639 2.2:+50406730 MS.gene58817:CDS
CGATAAGATTCCAGAAATCT+TGG 0.378676 2.2:+50406282 MS.gene58817:CDS
ATGATTACATTGTTAAAGTT+CGG 0.389938 2.2:+50406782 MS.gene58817:CDS
CACACGCTCCTTGTAGTTTG+AGG 0.390945 2.2:-50406204 None:intergenic
CGGCTAAGTGGACCGGCAGC+AGG 0.440486 2.2:+50406802 MS.gene58817:CDS
AGCTGCACAGCAGCAATAGA+AGG 0.442231 2.2:-50406874 None:intergenic
TGAAGTAAGGCCTGGTTTGA+TGG 0.459751 2.2:+50406132 MS.gene58817:CDS
CTCAACCATGACATTGAAGA+TGG 0.471530 2.2:+50406240 MS.gene58817:CDS
ATTGTTAAAGTTCGGCTAAG+TGG 0.495444 2.2:+50406790 MS.gene58817:CDS
TCCTTGAAGAATCTTATAGC+AGG 0.507331 2.2:+50405487 None:intergenic
GACCATATCTTATTGGAACT+GGG 0.523059 2.2:+50406731 MS.gene58817:CDS
CATTCTCTTCTGTCAGTGGC+AGG 0.536464 2.2:-50406093 None:intergenic
TCTACATTCTCTTCTGTCAG+TGG 0.539215 2.2:-50406097 None:intergenic
GATCCCCATCTTCAATGTCA+TGG 0.540108 2.2:-50406245 None:intergenic
TAAAGTTCGGCTAAGTGGAC+CGG 0.546274 2.2:+50406795 MS.gene58817:CDS
CCTCCATCAGCCATCAAACC+AGG 0.556402 2.2:-50406142 None:intergenic
GTTGTTATCCTCAAACTACA+AGG 0.559144 2.2:+50406196 MS.gene58817:CDS
GTGGCATTGCATGAGATAGA+TGG 0.565280 2.2:+50406169 MS.gene58817:CDS
TTCAATGTCATGGTTGAGCT+AGG 0.567639 2.2:-50406235 None:intergenic
TCAACCATGACATTGAAGAT+GGG 0.574515 2.2:+50406241 MS.gene58817:CDS
CAGATACTAGATACTGAGAC+TGG 0.580123 2.2:+50406313 MS.gene58817:CDS
TAAGATTCCAGAAATCTTGG+AGG 0.581548 2.2:+50406285 MS.gene58817:CDS
CTGGTTTGATGGCTGATGGA+GGG 0.590897 2.2:+50406143 MS.gene58817:CDS
CCTGGTTTGATGGCTGATGG+AGG 0.592266 2.2:+50406142 MS.gene58817:CDS
GTCAGTGGCAGGACACATCT+CGG 0.597217 2.2:-50406082 None:intergenic
ACCATATCTTATTGGAACTG+GGG 0.604069 2.2:+50406732 MS.gene58817:CDS
CCATATCTTATTGGAACTGG+GGG 0.604476 2.2:+50406733 MS.gene58817:CDS
GATGGCTGATGGAGGGAATG+TGG 0.613469 2.2:+50406150 MS.gene58817:CDS
AGGCCTGGTTTGATGGCTGA+TGG 0.614038 2.2:+50406139 MS.gene58817:CDS
CGTGCTCCAAGAAACAAAGC+AGG 0.615747 2.2:+50405595 MS.gene58817:CDS
CAACCATGACATTGAAGATG+GGG 0.616574 2.2:+50406242 MS.gene58817:CDS
ACTGTCATAACTCCTGCTGC+CGG 0.622897 2.2:-50406814 None:intergenic
CTCAAACTACAAGGAGCGTG+TGG 0.626569 2.2:+50406205 MS.gene58817:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCATGAATTCATATTA+AGG - chr2.2:50405782-50405801 None:intergenic 15.0%
!!! ATAAAATTATTCATTTTTCC+AGG + chr2.2:50406693-50406712 MS.gene58817:intron 15.0%
!!! ATTAATATTAAGCAAGTTTT+CGG + chr2.2:50405559-50405578 MS.gene58817:CDS 15.0%
!!! GTTAAATTTTAATCAATTCT+TGG + chr2.2:50405664-50405683 MS.gene58817:intron 15.0%
!! ATGATTACATTGTTAAAGTT+CGG + chr2.2:50406782-50406801 MS.gene58817:CDS 20.0%
!! GTGATATACAAATGAAATTA+TGG - chr2.2:50406408-50406427 None:intergenic 20.0%
!!! AATCAATTCTTGGATTTTAT+TGG + chr2.2:50405674-50405693 MS.gene58817:intron 20.0%
!!! TTAATATTAAGCAAGTTTTC+GGG + chr2.2:50405560-50405579 MS.gene58817:CDS 20.0%
! AATTTCTCTACAAACATGTA+AGG - chr2.2:50406577-50406596 None:intergenic 25.0%
! GAAATTAGACCATATCTTAT+TGG + chr2.2:50406724-50406743 MS.gene58817:CDS 25.0%
AATGCAAGAAATGATGCATA+AGG - chr2.2:50406384-50406403 None:intergenic 30.0%
ACTAAGTTGTTTATCCAAAC+AGG + chr2.2:50406517-50406536 MS.gene58817:intron 30.0%
! ACGCTATTCTTGAGATTTTA+TGG - chr2.2:50406462-50406481 None:intergenic 30.0%
! AGAGAATGTAGAGAAAGTTT+TGG + chr2.2:50406108-50406127 MS.gene58817:CDS 30.0%
! ATGTCGAAAGTGTATGTAAT+TGG + chr2.2:50406356-50406375 MS.gene58817:intron 30.0%
! CGCTATTCTTGAGATTTTAT+GGG - chr2.2:50406461-50406480 None:intergenic 30.0%
! TACACTTTCGACATTTTCTT+CGG - chr2.2:50406350-50406369 None:intergenic 30.0%
!!! AGAAAGTTTTGGATGAAGTA+AGG + chr2.2:50406119-50406138 MS.gene58817:CDS 30.0%
!!! CATTGCAATGTTTTTTTTGC+AGG + chr2.2:50406046-50406065 MS.gene58817:intron 30.0%
ACCATATCTTATTGGAACTG+GGG + chr2.2:50406732-50406751 MS.gene58817:CDS 35.0%
AGACCATATCTTATTGGAAC+TGG + chr2.2:50406730-50406749 MS.gene58817:CDS 35.0%
AGTTAAGCACTTACCTTATC+AGG - chr2.2:50406500-50406519 None:intergenic 35.0%
ATTGTTAAAGTTCGGCTAAG+TGG + chr2.2:50406790-50406809 MS.gene58817:CDS 35.0%
CGATAAGATTCCAGAAATCT+TGG + chr2.2:50406282-50406301 MS.gene58817:CDS 35.0%
GAAAACAGTTTGACCTGATA+AGG + chr2.2:50406484-50406503 MS.gene58817:intron 35.0%
GACCATATCTTATTGGAACT+GGG + chr2.2:50406731-50406750 MS.gene58817:CDS 35.0%
GTTGTTATCCTCAAACTACA+AGG + chr2.2:50406196-50406215 MS.gene58817:CDS 35.0%
TAAGATTCCAGAAATCTTGG+AGG + chr2.2:50406285-50406304 MS.gene58817:CDS 35.0%
TCAACCATGACATTGAAGAT+GGG + chr2.2:50406241-50406260 MS.gene58817:CDS 35.0%
TTGTGAACTTAGATCCTGTT+TGG - chr2.2:50406534-50406553 None:intergenic 35.0%
! AATGAGTGTTGTGTCTCTAT+TGG - chr2.2:50405828-50405847 None:intergenic 35.0%
! ATCTAATCAAGTAGCTTGCA+AGG + chr2.2:50405992-50406011 MS.gene58817:intron 35.0%
! GTCTAATTTCTGAAAGAACC+TGG - chr2.2:50406714-50406733 None:intergenic 35.0%
! TCTAATCAAGTAGCTTGCAA+GGG + chr2.2:50405993-50406012 MS.gene58817:intron 35.0%
!! AATGAGAGTTGTTTTCTCAG+AGG + chr2.2:50405529-50405548 MS.gene58817:CDS 35.0%
AACATGTGTCAGTGTCATGT+TGG + chr2.2:50405895-50405914 MS.gene58817:intron 40.0%
CAACCATGACATTGAAGATG+GGG + chr2.2:50406242-50406261 MS.gene58817:CDS 40.0%
CACAACACTCATTGACATGT+AGG + chr2.2:50405835-50405854 MS.gene58817:intron 40.0%
CAGATACTAGATACTGAGAC+TGG + chr2.2:50406313-50406332 MS.gene58817:CDS 40.0%
CCATATCTTATTGGAACTGG+GGG + chr2.2:50406733-50406752 MS.gene58817:CDS 40.0%
CTCAACCATGACATTGAAGA+TGG + chr2.2:50406240-50406259 MS.gene58817:CDS 40.0%
TCTACATTCTCTTCTGTCAG+TGG - chr2.2:50406100-50406119 None:intergenic 40.0%
TGTTCAACCTCCAAGATTTC+TGG - chr2.2:50406295-50406314 None:intergenic 40.0%
TTCAATGTCATGGTTGAGCT+AGG - chr2.2:50406238-50406257 None:intergenic 40.0%
!! GAAGAACCTGCTTTGTTTCT+TGG - chr2.2:50405604-50405623 None:intergenic 40.0%
CCAAACACGTCGTCAATCTA+AGG + chr2.2:50405944-50405963 MS.gene58817:intron 45.0%
CCCCCAGTTCCAATAAGATA+TGG - chr2.2:50406736-50406755 None:intergenic 45.0%
CCTTAGATTGACGACGTGTT+TGG - chr2.2:50405947-50405966 None:intergenic 45.0%
GATCCCCATCTTCAATGTCA+TGG - chr2.2:50406248-50406267 None:intergenic 45.0%
GTGGCATTGCATGAGATAGA+TGG + chr2.2:50406169-50406188 MS.gene58817:CDS 45.0%
TAAAGTTCGGCTAAGTGGAC+CGG + chr2.2:50406795-50406814 MS.gene58817:CDS 45.0%
TATCTTATTGGAACTGGGGG+AGG + chr2.2:50406736-50406755 MS.gene58817:CDS 45.0%
! TGAAGTAAGGCCTGGTTTGA+TGG + chr2.2:50406132-50406151 MS.gene58817:CDS 45.0%
! TTGGTGTCTGACATGTGTCA+GGG - chr2.2:50405928-50405947 None:intergenic 45.0%
!! GTTTTGGATGAAGTAAGGCC+TGG + chr2.2:50406124-50406143 MS.gene58817:CDS 45.0%
!! TTTGGTGTCTGACATGTGTC+AGG - chr2.2:50405929-50405948 None:intergenic 45.0%
ACTGTCATAACTCCTGCTGC+CGG - chr2.2:50406817-50406836 None:intergenic 50.0%
AGCTGCACAGCAGCAATAGA+AGG - chr2.2:50406877-50406896 None:intergenic 50.0%
CACACGCTCCTTGTAGTTTG+AGG - chr2.2:50406207-50406226 None:intergenic 50.0%
CATTCTCTTCTGTCAGTGGC+AGG - chr2.2:50406096-50406115 None:intergenic 50.0%
CGTGCTCCAAGAAACAAAGC+AGG + chr2.2:50405595-50405614 MS.gene58817:CDS 50.0%
! CTCAAACTACAAGGAGCGTG+TGG + chr2.2:50406205-50406224 MS.gene58817:CDS 50.0%
! CTGGTTTGATGGCTGATGGA+GGG + chr2.2:50406143-50406162 MS.gene58817:CDS 50.0%
CCTCCATCAGCCATCAAACC+AGG - chr2.2:50406145-50406164 None:intergenic 55.0%
GATGGCTGATGGAGGGAATG+TGG + chr2.2:50406150-50406169 MS.gene58817:CDS 55.0%
GTCAGTGGCAGGACACATCT+CGG - chr2.2:50406085-50406104 None:intergenic 55.0%
! AGGCCTGGTTTGATGGCTGA+TGG + chr2.2:50406139-50406158 MS.gene58817:CDS 55.0%
! CCTGGTTTGATGGCTGATGG+AGG + chr2.2:50406142-50406161 MS.gene58817:CDS 55.0%
CGGCTAAGTGGACCGGCAGC+AGG + chr2.2:50406802-50406821 MS.gene58817:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 50405496 50406906 50405496 ID=MS.gene58817
chr2.2 mRNA 50405496 50406906 50405496 ID=MS.gene58817.t1;Parent=MS.gene58817
chr2.2 exon 50405496 50405616 50405496 ID=MS.gene58817.t1.exon1;Parent=MS.gene58817.t1
chr2.2 CDS 50405496 50405616 50405496 ID=cds.MS.gene58817.t1;Parent=MS.gene58817.t1
chr2.2 exon 50406068 50406366 50406068 ID=MS.gene58817.t1.exon2;Parent=MS.gene58817.t1
chr2.2 CDS 50406068 50406366 50406068 ID=cds.MS.gene58817.t1;Parent=MS.gene58817.t1
chr2.2 exon 50406715 50406906 50406715 ID=MS.gene58817.t1.exon3;Parent=MS.gene58817.t1
chr2.2 CDS 50406715 50406906 50406715 ID=cds.MS.gene58817.t1;Parent=MS.gene58817.t1
Gene Sequence

>MS.gene58817

AATCTTATAGCAGGTAATAGAAGATTTTCATCAAATGAGAGTTGTTTTCTCAGAGGTCATCAAATTAATATTAAGCAAGTTTTCGGGCTAATCTCTCGTCGTGCTCCAAGAAACAAAGCAGGTTCTTCTAGAGTTTCATCAATTTGAATATACTTGTATTCATTATTTGTTAAATTTTAATCAATTCTTGGATTTTATTGGATGAAAGTTAGTACTAGTAGTTTAGTATAATACATTTCAATTGTTTAGCACTAAGATAATTGAAAGTGTGACTGATTTTGTTCCTTAATATGAATTCATGATTTTAACAAATTGCAATCTATGAAGCACCAATAGAGACACAACACTCATTGACATGTAGGTTTAGTAATAATTTAAAAAATACAAGTGATTAAACGTAACATGTGTCAGTGTCATGTTGGTGTCAGACCCTGACACATGTCAGACACCAAACACGTCGTCAATCTAAGGTGACAGTGCTACATAGATTGCAATCATCTAATCAAGTAGCTTGCAAGGGATGTGAGAAAATAGTTGAATCTAGCATTCACATTGCAATGTTTTTTTTGCAGGACTTTTTGTTTCTCCGAGATGTGTCCTGCCACTGACAGAAGAGAATGTAGAGAAAGTTTTGGATGAAGTAAGGCCTGGTTTGATGGCTGATGGAGGGAATGTGGCATTGCATGAGATAGATGGTCTTGTTGTTATCCTCAAACTACAAGGAGCGTGTGGATCATGTCCTAGCTCAACCATGACATTGAAGATGGGGATCGAAACTCGTCTTCGCGATAAGATTCCAGAAATCTTGGAGGTTGAACAGATACTAGATACTGAGACTGGTCTTGAGCTAACCGAAGAAAATGTCGAAAGTGTATGTAATTGGAACCTTATGCATCATTTCTTGCATTGCCATAATTTCATTTGTATATCACTTACTTCGTAAAAAAACATAAAAGCTTCTTCCCATAAAATCTCAAGAATAGCGTGTGAAAACAGTTTGACCTGATAAGGTAAGTGCTTAACTAAGTTGTTTATCCAAACAGGATCTAAGTTCACAAATATTCTCATATTTATAGTGCCTTACATGTTTGTAGAGAAATTATCTATAAGAAAACATTGACATGTATCTATATAAGCTGAATAATTATGTTGCTAGTGACAACTTCTTGTTGCTTTAATGTAAAGATTGCGAATCATATAAAATTATTCATTTTTCCAGGTTCTTTCAGAAATTAGACCATATCTTATTGGAACTGGGGGAGGAACATTAGAGCTTGTTGAAATTAATGATTACATTGTTAAAGTTCGGCTAAGTGGACCGGCAGCAGGAGTTATGACAGTTCGTGTTGCGTTAACGCAGAAGCTGAGAGACAAGATACCTTCTATTGCTGCTGTGCAGCTAATAGAATGA

Protein sequence

>MS.gene58817.t1

NLIAGNRRFSSNESCFLRGHQINIKQVFGLISRRAPRNKAGLFVSPRCVLPLTEENVEKVLDEVRPGLMADGGNVALHEIDGLVVILKLQGACGSCPSSTMTLKMGIETRLRDKIPEILEVEQILDTETGLELTEENVESVLSEIRPYLIGTGGGTLELVEINDYIVKVRLSGPAAGVMTVRVALTQKLRDKIPSIAAVQLIE