AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90135


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90135.t1 MTR_2g081090 96.774 62 2 0 1 62 34 95 9.87e-38 125
MS.gene90135.t1 MTR_2g081090 96.296 54 2 0 9 62 15 68 1.10e-31 109
MS.gene90135.t1 MTR_5g097280 69.565 69 14 1 1 62 30 98 6.34e-26 95.5
MS.gene90135.t1 MTR_4g094605 65.753 73 14 1 1 62 27 99 9.99e-26 94.7
MS.gene90135.t1 MTR_6g012080 89.583 48 5 0 15 62 51 98 1.35e-25 94.7
MS.gene90135.t1 MTR_6g012110 89.583 48 5 0 15 62 51 98 1.35e-25 94.7
MS.gene90135.t1 MTR_6g011880 89.583 48 5 0 15 62 51 98 1.65e-25 94.4
MS.gene90135.t1 MTR_6g011870 89.583 48 5 0 15 62 51 98 1.72e-25 94.4
MS.gene90135.t1 MTR_6g011890 87.500 48 6 0 15 62 51 98 4.53e-25 93.2
MS.gene90135.t1 MTR_6g043600 85.417 48 7 0 15 62 35 82 3.10e-24 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90135.t1 AT5G54270 85.484 62 9 0 1 62 35 96 8.00e-34 115
MS.gene90135.t1 AT2G05070 79.245 53 11 0 10 62 46 98 2.51e-24 91.3
MS.gene90135.t1 AT2G05100 82.353 51 9 0 12 62 48 98 2.65e-24 91.3
MS.gene90135.t1 AT1G29930 62.500 72 17 1 1 62 28 99 2.77e-24 91.3
MS.gene90135.t1 AT1G29920 62.500 72 17 1 1 62 28 99 2.92e-24 91.3
MS.gene90135.t1 AT1G29910 62.500 72 17 1 1 62 28 99 2.92e-24 91.3
MS.gene90135.t1 AT3G27690 79.245 53 11 0 10 62 47 99 3.19e-24 90.9
MS.gene90135.t1 AT3G27690 87.500 48 6 0 15 62 84 131 4.78e-24 91.3
MS.gene90135.t1 AT2G05100 79.245 53 11 0 10 62 46 98 5.75e-24 91.3
MS.gene90135.t1 AT2G34420 62.500 72 17 1 1 62 26 97 8.78e-24 89.7
MS.gene90135.t1 AT2G34430 85.417 48 7 0 15 62 51 98 1.29e-23 89.4

Find 24 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTCTTGGCAAATGCTTC+TGG 0.168930 2.3:-17345709 None:intergenic
ACCAGACAGAGTGAAATATT+TGG 0.277826 2.3:+17345600 MS.gene90135:CDS
GGAGAATTCCCTGGTGATTA+TGG 0.284769 2.3:+17345658 MS.gene90135:CDS
CCAGACAGAGTGAAATATTT+GGG 0.334825 2.3:+17345601 MS.gene90135:CDS
AACCTCAAGAGCCCTGTTCT+TGG 0.335763 2.3:-17345722 None:intergenic
CCCAAATATTTCACTCTGTC+TGG 0.371437 2.3:-17345601 None:intergenic
CAGAAGCATTTGCCAAGAAC+AGG 0.372548 2.3:+17345710 MS.gene90135:CDS
CAGACTCCTTCATACTTAAC+TGG 0.384985 2.3:+17345637 MS.gene90135:CDS
GAAGGAGTCTGAGCTGAAAA+TGG 0.411828 2.3:-17345625 None:intergenic
GAAGGGAAATGAATTGTGTA+TGG 0.465776 2.3:+17345576 MS.gene90135:intron
GGGAACCTCAAGATTCACTA+TGG 0.472897 2.3:+17344958 MS.gene90135:CDS
TGCCAAGAACAGGGCTCTTG+AGG 0.515098 2.3:+17345720 MS.gene90135:CDS
AATTCCCTGGTGATTATGGA+TGG 0.526464 2.3:+17345662 MS.gene90135:CDS
TACTTAACTGGAGAATTCCC+TGG 0.533200 2.3:+17345649 MS.gene90135:CDS
GATTATGGATGGGACACTGC+TGG 0.539551 2.3:+17345673 MS.gene90135:CDS
AATTCTCCAGTTAAGTATGA+AGG 0.558241 2.3:-17345643 None:intergenic
GTGTCCCATCCATAATCACC+AGG 0.560079 2.3:-17345667 None:intergenic
AGTGAATCTTGAGGTTCCCA+TGG 0.607906 2.3:-17344954 None:intergenic
ATTCCCTGGTGATTATGGAT+GGG 0.621810 2.3:+17345663 MS.gene90135:CDS
AGAAGCATTTGCCAAGAACA+GGG 0.625251 2.3:+17345711 MS.gene90135:CDS
AAGGGAAATGAATTGTGTAT+GGG 0.663509 2.3:+17345577 MS.gene90135:intron
AAATACCATAGTGAATCTTG+AGG 0.669239 2.3:-17344963 None:intergenic
CAGACAGAGTGAAATATTTG+GGG 0.720681 2.3:+17345602 MS.gene90135:CDS
TGTCCCATCCATAATCACCA+GGG 0.728458 2.3:-17345666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTATTAGATACAATAT+TGG - chr2.3:17345099-17345118 None:intergenic 10.0%
!!! GAAATTTTTACTAAAAAAAT+AGG - chr2.3:17344998-17345017 None:intergenic 10.0%
!!! AAAATCATACTTTTTTTTTG+AGG - chr2.3:17345141-17345160 None:intergenic 15.0%
!!! AAATCATACTTTTTTTTTGA+GGG - chr2.3:17345140-17345159 None:intergenic 15.0%
!!! ACGTTTAGATATGTTTTTAT+TGG + chr2.3:17345449-17345468 MS.gene90135:intron 20.0%
!!! CAAGTATGTTTTTTTATCAT+TGG + chr2.3:17345204-17345223 MS.gene90135:intron 20.0%
! ACTAAAAAAATAGGGAAAAG+AGG - chr2.3:17344989-17345008 None:intergenic 25.0%
! ATAAAAAAACATACTTGTGC+AGG - chr2.3:17345201-17345220 None:intergenic 25.0%
! CTAACAAAGTATATGCTTTA+TGG - chr2.3:17345334-17345353 None:intergenic 25.0%
!!! TTTATATAGTGTTGCTTATG+TGG + chr2.3:17345525-17345544 MS.gene90135:intron 25.0%
AAATACCATAGTGAATCTTG+AGG - chr2.3:17344966-17344985 None:intergenic 30.0%
AAATGTGTGATGCTACTAAT+TGG + chr2.3:17345054-17345073 MS.gene90135:intron 30.0%
AAGGGAAATGAATTGTGTAT+GGG + chr2.3:17345577-17345596 MS.gene90135:intron 30.0%
AATTCTCCAGTTAAGTATGA+AGG - chr2.3:17345646-17345665 None:intergenic 30.0%
ATGCTTTATGGCCAATTATA+TGG - chr2.3:17345322-17345341 None:intergenic 30.0%
ATTGTGTTTGTGATTGTGAA+GGG + chr2.3:17345559-17345578 MS.gene90135:intron 30.0%
TAACTAATGATAGACACGTT+CGG + chr2.3:17345405-17345424 MS.gene90135:intron 30.0%
TTAGTAGCATCACACATTTA+AGG - chr2.3:17345053-17345072 None:intergenic 30.0%
TTTGTTTCGCACCATATAAT+TGG + chr2.3:17345308-17345327 MS.gene90135:intron 30.0%
! AGACTAAATTGACTCAAGTT+AGG + chr2.3:17345363-17345382 MS.gene90135:intron 30.0%
AACTAATGATAGACACGTTC+GGG + chr2.3:17345406-17345425 MS.gene90135:intron 35.0%
ACCAGACAGAGTGAAATATT+TGG + chr2.3:17345600-17345619 MS.gene90135:CDS 35.0%
CAGACAGAGTGAAATATTTG+GGG + chr2.3:17345602-17345621 MS.gene90135:CDS 35.0%
CATTGTGTTTGTGATTGTGA+AGG + chr2.3:17345558-17345577 MS.gene90135:intron 35.0%
CCAGACAGAGTGAAATATTT+GGG + chr2.3:17345601-17345620 MS.gene90135:CDS 35.0%
GAAGGGAAATGAATTGTGTA+TGG + chr2.3:17345576-17345595 MS.gene90135:intron 35.0%
AATTCCCTGGTGATTATGGA+TGG + chr2.3:17345662-17345681 MS.gene90135:CDS 40.0%
ATTCCCTGGTGATTATGGAT+GGG + chr2.3:17345663-17345682 MS.gene90135:CDS 40.0%
CAGACTCCTTCATACTTAAC+TGG + chr2.3:17345637-17345656 MS.gene90135:CDS 40.0%
CCCAAATATTTCACTCTGTC+TGG - chr2.3:17345604-17345623 None:intergenic 40.0%
TACTTAACTGGAGAATTCCC+TGG + chr2.3:17345649-17345668 MS.gene90135:CDS 40.0%
!! AGAAGCATTTGCCAAGAACA+GGG + chr2.3:17345711-17345730 MS.gene90135:CDS 40.0%
CTGTTCTTGGCAAATGCTTC+TGG - chr2.3:17345712-17345731 None:intergenic 45.0%
GAAGGAGTCTGAGCTGAAAA+TGG - chr2.3:17345628-17345647 None:intergenic 45.0%
GGAGAATTCCCTGGTGATTA+TGG + chr2.3:17345658-17345677 MS.gene90135:CDS 45.0%
GGGAACCTCAAGATTCACTA+TGG + chr2.3:17344958-17344977 MS.gene90135:CDS 45.0%
TGTCCCATCCATAATCACCA+GGG - chr2.3:17345669-17345688 None:intergenic 45.0%
!! CAGAAGCATTTGCCAAGAAC+AGG + chr2.3:17345710-17345729 MS.gene90135:CDS 45.0%
!!! AAATTTTTACTAAAAAAATA+GGG - chr2.3:17344997-17345016 None:intergenic 5.0%
GATTATGGATGGGACACTGC+TGG + chr2.3:17345673-17345692 MS.gene90135:CDS 50.0%
GTGTCCCATCCATAATCACC+AGG - chr2.3:17345670-17345689 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 17344956 17345741 17344956 ID=MS.gene90135
chr2.3 mRNA 17344956 17345741 17344956 ID=MS.gene90135.t1;Parent=MS.gene90135
chr2.3 exon 17344956 17344979 17344956 ID=MS.gene90135.t1.exon1;Parent=MS.gene90135.t1
chr2.3 CDS 17344956 17344979 17344956 ID=cds.MS.gene90135.t1;Parent=MS.gene90135.t1
chr2.3 exon 17345580 17345741 17345580 ID=MS.gene90135.t1.exon2;Parent=MS.gene90135.t1
chr2.3 CDS 17345580 17345741 17345580 ID=cds.MS.gene90135.t1;Parent=MS.gene90135.t1
Gene Sequence

>MS.gene90135

ATGGGAACCTCAAGATTCACTATGGTATTTCCTCTTTTCCCTATTTTTTTAGTAAAAATTTCACTATATTTATAGAATAAACATTCATTTTAGTCCTTAAATGTGTGATGCTACTAATTGGTCTAACTAACTTAGTGTGACCAATATTGTATCTAATAATTAATAAAGTATGATTTTGTCTCCCTCAAAAAAAAAGTATGATTTTGTCTAATGTGCACACAAGTAAAATTCACTTGCTTGCACCTGCACAAGTATGTTTTTTTATCATTGGATCAATAAGTAGCATCATATTTCATTCAATTTAATAGTGAGATTTATTGATCAAATTGTAAAACTTATGCATGATGCAAGATTTGTTTCGCACCATATAATTGGCCATAAAGCATATACTTTGTTAGTAATGTTATAGACTAAATTGACTCAAGTTAGGACACATTCAAAAACTAAACTAACTAATGATAGACACGTTCGGGAGTAAAATGACTATAAAAACACGTTTAGATATGTTTTTATTGGTTATACATGCAATGACTAGCACATTTAAGTAGTGAAATAATTGTCTACTTGTATTTATATAGTGTTGCTTATGTGGCTGATACATGCATTGTGTTTGTGATTGTGAAGGGAAATGAATTGTGTATGGGACCAGACAGAGTGAAATATTTGGGGCCATTTTCAGCTCAGACTCCTTCATACTTAACTGGAGAATTCCCTGGTGATTATGGATGGGACACTGCTGGTTTATCTGCTGATCCAGAAGCATTTGCCAAGAACAGGGCTCTTGAG

Protein sequence

>MS.gene90135.t1

MGTSRFTMGNELCMGPDRVKYLGPFSAQTPSYLTGEFPGDYGWDTAGLSADPEAFAKNRALE