Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73334.t1 | AES65208.1 | 98.2 | 163 | 3 | 0 | 22 | 184 | 80 | 242 | 2.00E-80 | 308.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73334.t1 | Q84RQ7 | 82.8 | 157 | 26 | 1 | 29 | 184 | 80 | 236 | 1.7e-67 | 256.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73334.t1 | G7IN05 | 98.2 | 163 | 3 | 0 | 22 | 184 | 80 | 242 | 1.5e-80 | 308.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene73334 | 0.860068 | 2.67E-63 | -1.69E-46 |
| MS.gene049171 | MS.gene73334 | 0.815674 | 8.19E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene73334 | 0.81734 | 3.47E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene73334 | 0.822391 | 2.43E-53 | -1.69E-46 |
| MS.gene049351 | MS.gene73334 | 0.80661 | 7.57E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene73334 | 0.836426 | 9.43E-57 | -1.69E-46 |
| MS.gene049369 | MS.gene73334 | 0.835729 | 1.42E-56 | -1.69E-46 |
| MS.gene049396 | MS.gene73334 | 0.808381 | 3.19E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene73334 | 0.831333 | 1.77E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene73334 | 0.871167 | 8.40E-67 | -1.69E-46 |
| MS.gene049615 | MS.gene73334 | 0.823908 | 1.08E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene73334 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene73334 | 0.871285 | 7.68E-67 | -1.69E-46 |
| MS.gene050397 | MS.gene73334 | 0.826659 | 2.40E-54 | -1.69E-46 |
| MS.gene050400 | MS.gene73334 | 0.811905 | 5.54E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene73334 | 0.833335 | 5.66E-56 | -1.69E-46 |
| MS.gene050538 | MS.gene73334 | 0.802768 | 4.80E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene73334 | 0.882032 | 1.46E-70 | -1.69E-46 |
| MS.gene050670 | MS.gene73334 | 0.870478 | 1.42E-66 | -1.69E-46 |
| MS.gene050686 | MS.gene73334 | 0.820574 | 6.38E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene73334 | 0.833438 | 5.33E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene73334 | 0.865205 | 6.99E-65 | -1.69E-46 |
| MS.gene05081 | MS.gene73334 | 0.806019 | 1.01E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene73334 | 0.815324 | 9.80E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene73334 | 0.863293 | 2.76E-64 | -1.69E-46 |
| MS.gene051228 | MS.gene73334 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene051255 | MS.gene73334 | 0.80168 | 8.03E-49 | -1.69E-46 |
| MS.gene051360 | MS.gene73334 | 0.807271 | 5.48E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene73334 | 0.876173 | 1.72E-68 | -1.69E-46 |
| MS.gene051525 | MS.gene73334 | 0.813405 | 2.60E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene73334 | 0.881509 | 2.26E-70 | -1.69E-46 |
| MS.gene051692 | MS.gene73334 | 0.891519 | 3.69E-74 | -1.69E-46 |
| MS.gene051694 | MS.gene73334 | 0.832334 | 1.00E-55 | -1.69E-46 |
| MS.gene051734 | MS.gene73334 | 0.819115 | 1.38E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene73334 | 0.817388 | 3.38E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene73334 | 0.840981 | 6.27E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene73334 | 0.803697 | 3.08E-49 | -1.69E-46 |
| MS.gene051877 | MS.gene73334 | 0.824216 | 9.10E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene73334 | 0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene73334 | 0.810472 | 1.13E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene73334 | 0.825009 | 5.92E-54 | -1.69E-46 |
| MS.gene052322 | MS.gene73334 | 0.868626 | 5.68E-66 | -1.69E-46 |
| MS.gene052346 | MS.gene73334 | 0.814038 | 1.89E-51 | -1.69E-46 |
| MS.gene052414 | MS.gene73334 | 0.836269 | 1.03E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene73334 | 0.84682 | 1.71E-59 | -1.69E-46 |
| MS.gene052481 | MS.gene73334 | 0.877855 | 4.50E-69 | -1.69E-46 |
| MS.gene052484 | MS.gene73334 | 0.866057 | 3.77E-65 | -1.69E-46 |
| MS.gene052485 | MS.gene73334 | 0.857201 | 1.92E-62 | -1.69E-46 |
| MS.gene052501 | MS.gene73334 | 0.846004 | 2.85E-59 | -1.69E-46 |
| MS.gene052503 | MS.gene73334 | 0.819859 | 9.31E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene73334 | 0.833776 | 4.39E-56 | -1.69E-46 |
| MS.gene052506 | MS.gene73334 | 0.803349 | 3.64E-49 | -1.69E-46 |
| MS.gene052508 | MS.gene73334 | 0.81946 | 1.15E-52 | -1.69E-46 |
| MS.gene052510 | MS.gene73334 | 0.814866 | 1.24E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene73334 | 0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene052820 | MS.gene73334 | 0.805555 | 1.26E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene73334 | 0.806959 | 6.39E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene73334 | 0.813857 | 2.07E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene73334 | 0.809929 | 1.48E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene73334 | 0.806432 | 8.25E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene73334 | 0.843089 | 1.74E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene73334 | 0.854404 | 1.26E-61 | -1.69E-46 |
| MS.gene053104 | MS.gene73334 | 0.867586 | 1.23E-65 | -1.69E-46 |
| MS.gene053107 | MS.gene73334 | 0.825147 | 5.49E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene73334 | 0.801011 | 1.10E-48 | -1.69E-46 |
| MS.gene053207 | MS.gene73334 | 0.849597 | 2.92E-60 | -1.69E-46 |
| MS.gene053209 | MS.gene73334 | 0.834057 | 3.74E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene73334 | 0.836957 | 6.90E-57 | -1.69E-46 |
| MS.gene053286 | MS.gene73334 | 0.824682 | 7.07E-54 | -1.69E-46 |
| MS.gene053322 | MS.gene73334 | 0.819147 | 1.35E-52 | -1.69E-46 |
| MS.gene053436 | MS.gene73334 | 0.814135 | 1.80E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene73334 | 0.894839 | 1.69E-75 | -1.69E-46 |
| MS.gene053574 | MS.gene73334 | 0.844292 | 8.28E-59 | -1.69E-46 |
| MS.gene053632 | MS.gene73334 | 0.819432 | 1.17E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene73334 | 0.828333 | 9.53E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene73334 | 0.825786 | 3.88E-54 | -1.69E-46 |
| MS.gene053912 | MS.gene73334 | 0.800802 | 1.22E-48 | -1.69E-46 |
| MS.gene053916 | MS.gene73334 | 0.831536 | 1.58E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene73334 | 0.813944 | 1.98E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene73334 | 0.836227 | 1.06E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene73334 | 0.81974 | 9.91E-53 | -1.69E-46 |
| MS.gene054927 | MS.gene73334 | 0.826097 | 3.27E-54 | -1.69E-46 |
| MS.gene054955 | MS.gene73334 | 0.820086 | 8.26E-53 | -1.69E-46 |
| MS.gene05505 | MS.gene73334 | 0.849845 | 2.49E-60 | -1.69E-46 |
| MS.gene055116 | MS.gene73334 | 0.848691 | 5.22E-60 | -1.69E-46 |
| MS.gene055206 | MS.gene73334 | 0.853884 | 1.78E-61 | -1.69E-46 |
| MS.gene055224 | MS.gene73334 | 0.824187 | 9.25E-54 | -1.69E-46 |
| MS.gene055277 | MS.gene73334 | 0.807159 | 5.79E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene73334 | 0.855319 | 6.83E-62 | -1.69E-46 |
| MS.gene055362 | MS.gene73334 | 0.810247 | 1.27E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene73334 | 0.831166 | 1.95E-55 | -1.69E-46 |
| MS.gene055647 | MS.gene73334 | 0.824575 | 7.49E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene73334 | 0.86576 | 4.67E-65 | -1.69E-46 |
| MS.gene055909 | MS.gene73334 | 0.823618 | 1.26E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene73334 | 0.827271 | 1.72E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene73334 | 0.869837 | 2.30E-66 | -1.69E-46 |
| MS.gene055941 | MS.gene73334 | 0.848148 | 7.37E-60 | -1.69E-46 |
| MS.gene056166 | MS.gene73334 | 0.806263 | 8.96E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene73334 | 0.818009 | 2.45E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene73334 | 0.847476 | 1.13E-59 | -1.69E-46 |
| MS.gene056407 | MS.gene73334 | 0.848724 | 5.11E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene73334 | 0.85377 | 1.92E-61 | -1.69E-46 |
| MS.gene056422 | MS.gene73334 | 0.841432 | 4.77E-58 | -1.69E-46 |
| MS.gene056448 | MS.gene73334 | 0.809349 | 1.98E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene73334 | 0.869519 | 2.91E-66 | -1.69E-46 |
| MS.gene05664 | MS.gene73334 | 0.870702 | 1.20E-66 | -1.69E-46 |
| MS.gene05666 | MS.gene73334 | 0.846217 | 2.50E-59 | -1.69E-46 |
| MS.gene056796 | MS.gene73334 | 0.821046 | 4.97E-53 | -1.69E-46 |
| MS.gene056877 | MS.gene73334 | 0.804251 | 2.36E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene73334 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene73334 | 0.809098 | 2.24E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene73334 | 0.805413 | 1.35E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene73334 | 0.84715 | 1.39E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene73334 | 0.831998 | 1.21E-55 | -1.69E-46 |
| MS.gene057129 | MS.gene73334 | 0.831381 | 1.72E-55 | -1.69E-46 |
| MS.gene057146 | MS.gene73334 | 0.832571 | 8.76E-56 | -1.69E-46 |
| MS.gene057295 | MS.gene73334 | 0.810712 | 1.01E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene73334 | 0.869156 | 3.82E-66 | -1.69E-46 |
| MS.gene05735 | MS.gene73334 | 0.814269 | 1.68E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene73334 | 0.880947 | 3.61E-70 | -1.69E-46 |
| MS.gene057450 | MS.gene73334 | 0.800576 | 1.35E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene73334 | 0.813559 | 2.41E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene73334 | 0.859864 | 3.08E-63 | -1.69E-46 |
| MS.gene057749 | MS.gene73334 | 0.821272 | 4.41E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene73334 | 0.833894 | 4.10E-56 | -1.69E-46 |
| MS.gene057856 | MS.gene73334 | 0.865168 | 7.18E-65 | -1.69E-46 |
| MS.gene057857 | MS.gene73334 | 0.800898 | 1.16E-48 | -1.69E-46 |
| MS.gene057926 | MS.gene73334 | 0.859355 | 4.38E-63 | -1.69E-46 |
| MS.gene058006 | MS.gene73334 | 0.883095 | 5.99E-71 | -1.69E-46 |
| MS.gene058007 | MS.gene73334 | 0.803343 | 3.65E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene73334 | 0.868367 | 6.88E-66 | -1.69E-46 |
| MS.gene058293 | MS.gene73334 | 0.823894 | 1.08E-53 | -1.69E-46 |
| MS.gene058323 | MS.gene73334 | 0.825093 | 5.66E-54 | -1.69E-46 |
| MS.gene058376 | MS.gene73334 | 0.842708 | 2.19E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene73334 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene73334 | 0.80549 | 1.30E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene73334 | 0.801932 | 7.13E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene73334 | 0.823336 | 1.46E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene73334 | 0.824021 | 1.01E-53 | -1.69E-46 |
| MS.gene058787 | MS.gene73334 | 0.807448 | 5.03E-50 | -1.69E-46 |
| MS.gene058788 | MS.gene73334 | 0.826642 | 2.42E-54 | -1.69E-46 |
| MS.gene058790 | MS.gene73334 | 0.821623 | 3.66E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene73334 | 0.842787 | 2.09E-58 | -1.69E-46 |
| MS.gene058928 | MS.gene73334 | 0.812285 | 4.58E-51 | -1.69E-46 |
| MS.gene058948 | MS.gene73334 | 0.805201 | 1.50E-49 | -1.69E-46 |
| MS.gene058995 | MS.gene73334 | 0.817198 | 3.73E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene73334 | 0.804967 | 1.68E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene73334 | 0.828867 | 7.08E-55 | -1.69E-46 |
| MS.gene05913 | MS.gene73334 | 0.804175 | 2.45E-49 | -1.69E-46 |
| MS.gene059141 | MS.gene73334 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene73334 | 0.849831 | 2.51E-60 | -1.69E-46 |
| MS.gene059366 | MS.gene73334 | 0.809032 | 2.31E-50 | -1.69E-46 |
| MS.gene059368 | MS.gene73334 | 0.808604 | 2.85E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene73334 | 0.810954 | 8.92E-51 | -1.69E-46 |
| MS.gene059455 | MS.gene73334 | 0.849527 | 3.05E-60 | -1.69E-46 |
| MS.gene059536 | MS.gene73334 | 0.850135 | 2.07E-60 | -1.69E-46 |
| MS.gene059674 | MS.gene73334 | 0.814822 | 1.27E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene73334 | 0.832042 | 1.18E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene73334 | 0.857959 | 1.14E-62 | -1.69E-46 |
| MS.gene059692 | MS.gene73334 | 0.842182 | 3.02E-58 | -1.69E-46 |
| MS.gene059695 | MS.gene73334 | 0.84548 | 3.95E-59 | -1.69E-46 |
| MS.gene059711 | MS.gene73334 | 0.81903 | 1.44E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene73334 | 0.815649 | 8.30E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene73334 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene73334 | 0.823973 | 1.04E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene73334 | 0.83538 | 1.74E-56 | -1.69E-46 |
| MS.gene059933 | MS.gene73334 | 0.801369 | 9.30E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene73334 | 0.823629 | 1.25E-53 | -1.69E-46 |
| MS.gene060193 | MS.gene73334 | 0.853286 | 2.64E-61 | -1.69E-46 |
| MS.gene060403 | MS.gene73334 | 0.949926 | 4.72E-108 | -1.69E-46 |
| MS.gene060482 | MS.gene73334 | 0.836657 | 8.23E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene73334 | 0.8024 | 5.71E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene73334 | 0.877215 | 7.51E-69 | -1.69E-46 |
| MS.gene060818 | MS.gene73334 | 0.851455 | 8.78E-61 | -1.69E-46 |
| MS.gene060835 | MS.gene73334 | 0.858447 | 8.18E-63 | -1.69E-46 |
| MS.gene060837 | MS.gene73334 | 0.885837 | 5.76E-72 | -1.69E-46 |
| MS.gene06092 | MS.gene73334 | 0.857277 | 1.82E-62 | -1.69E-46 |
| MS.gene06097 | MS.gene73334 | 0.804509 | 2.09E-49 | -1.69E-46 |
| MS.gene060986 | MS.gene73334 | 0.814934 | 1.20E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene73334 | 0.838825 | 2.28E-57 | -1.69E-46 |
| MS.gene061051 | MS.gene73334 | -0.816231 | 6.15E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene73334 | 0.807617 | 4.63E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene73334 | 0.838212 | 3.29E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene73334 | 0.838159 | 3.39E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73334.t1 | MTR_2g036690 | 96.386 | 166 | 6 | 0 | 19 | 184 | 77 | 242 | 9.78e-111 | 316 |
| MS.gene73334.t1 | MTR_2g005900 | 73.418 | 158 | 42 | 0 | 26 | 183 | 66 | 223 | 3.24e-78 | 233 |
| MS.gene73334.t1 | MTR_2g005900 | 40.659 | 91 | 49 | 2 | 95 | 181 | 54 | 143 | 2.20e-11 | 60.8 |
| MS.gene73334.t1 | MTR_2g036690 | 97.030 | 101 | 3 | 0 | 22 | 122 | 80 | 180 | 1.78e-64 | 197 |
| MS.gene73334.t1 | MTR_7g109850 | 39.355 | 155 | 90 | 3 | 19 | 172 | 57 | 208 | 2.57e-25 | 97.8 |
| MS.gene73334.t1 | MTR_8g038785 | 48.148 | 81 | 40 | 1 | 19 | 99 | 57 | 135 | 3.07e-16 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73334.t1 | AT4G25910 | 80.368 | 163 | 31 | 1 | 23 | 184 | 74 | 236 | 1.22e-90 | 265 |
| MS.gene73334.t1 | AT5G49940 | 77.778 | 153 | 34 | 0 | 31 | 183 | 83 | 235 | 5.91e-81 | 240 |
| MS.gene73334.t1 | AT5G49940 | 76.087 | 92 | 22 | 0 | 31 | 122 | 83 | 174 | 7.15e-44 | 144 |
| MS.gene73334.t1 | AT5G49940 | 47.297 | 74 | 34 | 2 | 114 | 183 | 85 | 157 | 7.59e-12 | 61.6 |
| MS.gene73334.t1 | AT4G01940 | 35.263 | 190 | 103 | 6 | 1 | 172 | 36 | 223 | 4.06e-25 | 97.4 |
Find 37 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCAACCTCCAAGATTTC+TGG | 0.161640 | 2.4:-52791764 | None:intergenic |
| GAAATTAGACCATATCTTAT+TGG | 0.195631 | 2.4:+52792196 | MS.gene73334:CDS |
| AGAGAATGTAGAGAAAGTTT+TGG | 0.294107 | 2.4:+52791580 | MS.gene73334:CDS |
| AGACCATATCTTATTGGAAC+TGG | 0.334639 | 2.4:+52792202 | MS.gene73334:CDS |
| CGATAAGATTCCAGAAATCT+TGG | 0.378676 | 2.4:+52791754 | MS.gene73334:CDS |
| ATGATTACATTGTTAAAGTT+CGG | 0.389938 | 2.4:+52792254 | MS.gene73334:CDS |
| CACACGCTCCTTGTAGTTTG+AGG | 0.390945 | 2.4:-52791676 | None:intergenic |
| CGGCTAAGTGGACCGGCAGC+AGG | 0.440486 | 2.4:+52792274 | MS.gene73334:CDS |
| AGCTGCACAGCAGCAATAGA+AGG | 0.442231 | 2.4:-52792346 | None:intergenic |
| TGAAGTAAGGCCTGGTTTGA+TGG | 0.459751 | 2.4:+52791604 | MS.gene73334:CDS |
| CTCAACCATGACATTGAAGA+TGG | 0.471530 | 2.4:+52791712 | MS.gene73334:CDS |
| ATGAATTGTATTAGCAATCA+AGG | 0.474582 | 2.4:+52789868 | MS.gene73334:CDS |
| TATAATCATGTGCATGAGTT+GGG | 0.474826 | 2.4:+52789838 | MS.gene73334:CDS |
| CTATAATCATGTGCATGAGT+TGG | 0.475276 | 2.4:+52789837 | MS.gene73334:CDS |
| ATTGTTAAAGTTCGGCTAAG+TGG | 0.495444 | 2.4:+52792262 | MS.gene73334:CDS |
| GACCATATCTTATTGGAACT+GGG | 0.523059 | 2.4:+52792203 | MS.gene73334:CDS |
| CATTCTCTTCTGTCAGTGGC+AGG | 0.536464 | 2.4:-52791565 | None:intergenic |
| TCTACATTCTCTTCTGTCAG+TGG | 0.539215 | 2.4:-52791569 | None:intergenic |
| GATCCCCATCTTCAATGTCA+TGG | 0.540108 | 2.4:-52791717 | None:intergenic |
| TAAAGTTCGGCTAAGTGGAC+CGG | 0.546274 | 2.4:+52792267 | MS.gene73334:CDS |
| CCTCCATCAGCCATCAAACC+AGG | 0.556402 | 2.4:-52791614 | None:intergenic |
| GTTGTTATCCTCAAACTACA+AGG | 0.559144 | 2.4:+52791668 | MS.gene73334:CDS |
| GTGGCATTGCATGAGATAGA+TGG | 0.565280 | 2.4:+52791641 | MS.gene73334:CDS |
| TTCAATGTCATGGTTGAGCT+AGG | 0.567639 | 2.4:-52791707 | None:intergenic |
| TCAACCATGACATTGAAGAT+GGG | 0.574515 | 2.4:+52791713 | MS.gene73334:CDS |
| CAGATACTAGATACTGAGAC+TGG | 0.580123 | 2.4:+52791785 | MS.gene73334:CDS |
| TAAGATTCCAGAAATCTTGG+AGG | 0.581548 | 2.4:+52791757 | MS.gene73334:CDS |
| CTGGTTTGATGGCTGATGGA+GGG | 0.590897 | 2.4:+52791615 | MS.gene73334:CDS |
| CCTGGTTTGATGGCTGATGG+AGG | 0.592266 | 2.4:+52791614 | MS.gene73334:CDS |
| GTCAGTGGCAGGACACATCT+CGG | 0.597217 | 2.4:-52791554 | None:intergenic |
| ACCATATCTTATTGGAACTG+GGG | 0.604069 | 2.4:+52792204 | MS.gene73334:CDS |
| CCATATCTTATTGGAACTGG+GGG | 0.604476 | 2.4:+52792205 | MS.gene73334:CDS |
| GATGGCTGATGGAGGGAATG+TGG | 0.613469 | 2.4:+52791622 | MS.gene73334:CDS |
| AGGCCTGGTTTGATGGCTGA+TGG | 0.614038 | 2.4:+52791611 | MS.gene73334:CDS |
| CAACCATGACATTGAAGATG+GGG | 0.616574 | 2.4:+52791714 | MS.gene73334:CDS |
| ACTGTCATAACTCCTGCTGC+CGG | 0.622897 | 2.4:-52792286 | None:intergenic |
| CTCAAACTACAAGGAGCGTG+TGG | 0.626569 | 2.4:+52791677 | MS.gene73334:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAAACAAAATATAG+GGG | + | chr2.4:52790253-52790272 | MS.gene73334:intron | 10.0% |
| !!! | CAATATTTTATTTTACAAAA+AGG | + | chr2.4:52790535-52790554 | MS.gene73334:intron | 10.0% |
| !! | AAAATCATGAATTCATATTA+AGG | - | chr2.4:52791254-52791273 | None:intergenic | 15.0% |
| !! | TTTGTTCAGAAATTAATTTA+TGG | - | chr2.4:52790190-52790209 | None:intergenic | 15.0% |
| !!! | ATAAAATTATTCATTTTTCC+AGG | + | chr2.4:52792165-52792184 | MS.gene73334:intron | 15.0% |
| !!! | ATTAATATTAAGCAAGTTTT+CGG | + | chr2.4:52791031-52791050 | MS.gene73334:intron | 15.0% |
| !!! | GTTAAATTTTAATCAATTCT+TGG | + | chr2.4:52791136-52791155 | MS.gene73334:intron | 15.0% |
| !!! | TATACACTTATAAGATATTT+TGG | + | chr2.4:52790227-52790246 | MS.gene73334:intron | 15.0% |
| !! | AAAAAAAACAAGAAACATGA+AGG | - | chr2.4:52790761-52790780 | None:intergenic | 20.0% |
| !! | ATGATTACATTGTTAAAGTT+CGG | + | chr2.4:52792254-52792273 | MS.gene73334:CDS | 20.0% |
| !! | GTGATATACAAATGAAATTA+TGG | - | chr2.4:52791880-52791899 | None:intergenic | 20.0% |
| !! | TTCAATGTAATTATATGTGT+CGG | - | chr2.4:52790870-52790889 | None:intergenic | 20.0% |
| !!! | AATCAATTCTTGGATTTTAT+TGG | + | chr2.4:52791146-52791165 | MS.gene73334:intron | 20.0% |
| !!! | GAAAGTATTTTTTTCTTGAT+TGG | - | chr2.4:52790465-52790484 | None:intergenic | 20.0% |
| !!! | GTTAATTAATTTGTGATCTT+TGG | + | chr2.4:52789940-52789959 | MS.gene73334:intron | 20.0% |
| !!! | TGTATTTTTTTTTCTCTCTT+TGG | + | chr2.4:52790113-52790132 | MS.gene73334:intron | 20.0% |
| !!! | TTAATATTAAGCAAGTTTTC+GGG | + | chr2.4:52791032-52791051 | MS.gene73334:intron | 20.0% |
| !!! | TTTAGATGATATGAGTTTTT+GGG | - | chr2.4:52790360-52790379 | None:intergenic | 20.0% |
| !!! | TTTTAGATGATATGAGTTTT+TGG | - | chr2.4:52790361-52790380 | None:intergenic | 20.0% |
| ! | AATTATGTCTGAGTTTCATA+AGG | + | chr2.4:52790888-52790907 | MS.gene73334:intron | 25.0% |
| ! | AATTTCTCTACAAACATGTA+AGG | - | chr2.4:52792049-52792068 | None:intergenic | 25.0% |
| ! | ATGAATTGTATTAGCAATCA+AGG | + | chr2.4:52789868-52789887 | MS.gene73334:CDS | 25.0% |
| ! | ATTATTCACACTCAAATGTT+GGG | + | chr2.4:52790612-52790631 | MS.gene73334:intron | 25.0% |
| ! | GAAATTAGACCATATCTTAT+TGG | + | chr2.4:52792196-52792215 | MS.gene73334:CDS | 25.0% |
| !! | GAACACATAAATAAGTGTTT+TGG | - | chr2.4:52790168-52790187 | None:intergenic | 25.0% |
| !!! | GCGTATGTTATAATTTTAGT+TGG | + | chr2.4:52790066-52790085 | MS.gene73334:intron | 25.0% |
| !!! | GTGTCAATTGTTTTAGTTTT+TGG | - | chr2.4:52790516-52790535 | None:intergenic | 25.0% |
| !!! | TTGTTGTTTTTGTTGATTTG+AGG | - | chr2.4:52790668-52790687 | None:intergenic | 25.0% |
| AAGCTAATGACTAATGAGTT+AGG | + | chr2.4:52789984-52790003 | MS.gene73334:intron | 30.0% | |
| AATGCAAGAAATGATGCATA+AGG | - | chr2.4:52791856-52791875 | None:intergenic | 30.0% | |
| ACATCCTTTCCAACAAAATA+AGG | - | chr2.4:52790096-52790115 | None:intergenic | 30.0% | |
| ACTAAGTTGTTTATCCAAAC+AGG | + | chr2.4:52791989-52792008 | MS.gene73334:intron | 30.0% | |
| AGCTAATGACTAATGAGTTA+GGG | + | chr2.4:52789985-52790004 | MS.gene73334:intron | 30.0% | |
| ATCCTTTCCAACAAAATAAG+GGG | - | chr2.4:52790094-52790113 | None:intergenic | 30.0% | |
| ATTCTGTGACTCTTATAGTA+GGG | - | chr2.4:52790312-52790331 | None:intergenic | 30.0% | |
| CATCCTTTCCAACAAAATAA+GGG | - | chr2.4:52790095-52790114 | None:intergenic | 30.0% | |
| CATTATTCACACTCAAATGT+TGG | + | chr2.4:52790611-52790630 | MS.gene73334:intron | 30.0% | |
| CTTAAAGAGACAAAAATGTG+TGG | - | chr2.4:52790724-52790743 | None:intergenic | 30.0% | |
| TATAATCATGTGCATGAGTT+GGG | + | chr2.4:52789838-52789857 | MS.gene73334:CDS | 30.0% | |
| TCATTCTTATGATAGCTTTC+TGG | + | chr2.4:52790576-52790595 | MS.gene73334:intron | 30.0% | |
| ! | ACGCTATTCTTGAGATTTTA+TGG | - | chr2.4:52791934-52791953 | None:intergenic | 30.0% |
| ! | AGAGAATGTAGAGAAAGTTT+TGG | + | chr2.4:52791580-52791599 | MS.gene73334:CDS | 30.0% |
| ! | ATGTCGAAAGTGTATGTAAT+TGG | + | chr2.4:52791828-52791847 | MS.gene73334:intron | 30.0% |
| ! | CACACATTTTTGTCTCTTTA+AGG | + | chr2.4:52790722-52790741 | MS.gene73334:intron | 30.0% |
| ! | CGCTATTCTTGAGATTTTAT+GGG | - | chr2.4:52791933-52791952 | None:intergenic | 30.0% |
| ! | GTATGTGTGAGTTTTTCTTT+TGG | + | chr2.4:52790910-52790929 | MS.gene73334:intron | 30.0% |
| ! | TACACTTTCGACATTTTCTT+CGG | - | chr2.4:52791822-52791841 | None:intergenic | 30.0% |
| !! | AAGAAGTATTTGTGCTTCAT+AGG | - | chr2.4:52790805-52790824 | None:intergenic | 30.0% |
| !!! | AGAAAGTTTTGGATGAAGTA+AGG | + | chr2.4:52791591-52791610 | MS.gene73334:CDS | 30.0% |
| !!! | CATTGCAATGTTTTTTTTGC+AGG | + | chr2.4:52791518-52791537 | MS.gene73334:intron | 30.0% |
| AACAAGAGTTCCTAAGTTTG+TGG | - | chr2.4:52790412-52790431 | None:intergenic | 35.0% | |
| ACCATATCTTATTGGAACTG+GGG | + | chr2.4:52792204-52792223 | MS.gene73334:CDS | 35.0% | |
| ACCTGCTATAAGATTCTTCA+AGG | - | chr2.4:52790963-52790982 | None:intergenic | 35.0% | |
| AGACCATATCTTATTGGAAC+TGG | + | chr2.4:52792202-52792221 | MS.gene73334:CDS | 35.0% | |
| AGGGGCTTCATTAAGAAAAT+GGG | + | chr2.4:52790271-52790290 | MS.gene73334:intron | 35.0% | |
| AGTTAAGCACTTACCTTATC+AGG | - | chr2.4:52791972-52791991 | None:intergenic | 35.0% | |
| ATTGTTAAAGTTCGGCTAAG+TGG | + | chr2.4:52792262-52792281 | MS.gene73334:CDS | 35.0% | |
| CGATAAGATTCCAGAAATCT+TGG | + | chr2.4:52791754-52791773 | MS.gene73334:CDS | 35.0% | |
| CTATAATCATGTGCATGAGT+TGG | + | chr2.4:52789837-52789856 | MS.gene73334:CDS | 35.0% | |
| GAAAACAGTTTGACCTGATA+AGG | + | chr2.4:52791956-52791975 | MS.gene73334:intron | 35.0% | |
| GACCATATCTTATTGGAACT+GGG | + | chr2.4:52792203-52792222 | MS.gene73334:CDS | 35.0% | |
| GATTCTGTGACTCTTATAGT+AGG | - | chr2.4:52790313-52790332 | None:intergenic | 35.0% | |
| GCTAATGACTAATGAGTTAG+GGG | + | chr2.4:52789986-52790005 | MS.gene73334:intron | 35.0% | |
| GTGGACAATAATCTATGAGA+TGG | - | chr2.4:52790705-52790724 | None:intergenic | 35.0% | |
| GTTGTTATCCTCAAACTACA+AGG | + | chr2.4:52791668-52791687 | MS.gene73334:CDS | 35.0% | |
| TAAGATTCCAGAAATCTTGG+AGG | + | chr2.4:52791757-52791776 | MS.gene73334:CDS | 35.0% | |
| TAGGGGCTTCATTAAGAAAA+TGG | + | chr2.4:52790270-52790289 | MS.gene73334:intron | 35.0% | |
| TCAACCATGACATTGAAGAT+GGG | + | chr2.4:52791713-52791732 | MS.gene73334:CDS | 35.0% | |
| TCAGTTCTATCCACAAACTT+AGG | + | chr2.4:52790399-52790418 | MS.gene73334:intron | 35.0% | |
| TCCTTGAAGAATCTTATAGC+AGG | + | chr2.4:52790959-52790978 | MS.gene73334:intron | 35.0% | |
| TCTCTCTTTGGTACAAAGAA+AGG | + | chr2.4:52790125-52790144 | MS.gene73334:intron | 35.0% | |
| TTCTTGAATTAAGCGTGTCT+TGG | + | chr2.4:52790820-52790839 | MS.gene73334:intron | 35.0% | |
| TTGTGAACTTAGATCCTGTT+TGG | - | chr2.4:52792006-52792025 | None:intergenic | 35.0% | |
| ! | AATGAGTGTTGTGTCTCTAT+TGG | - | chr2.4:52791300-52791319 | None:intergenic | 35.0% |
| ! | ATCTAATCAAGTAGCTTGCA+AGG | + | chr2.4:52791464-52791483 | MS.gene73334:intron | 35.0% |
| ! | GCAAATTTGATTTCTCCTCT+AGG | + | chr2.4:52790334-52790353 | MS.gene73334:intron | 35.0% |
| ! | GTCTAATTTCTGAAAGAACC+TGG | - | chr2.4:52792186-52792205 | None:intergenic | 35.0% |
| ! | TCTAATCAAGTAGCTTGCAA+GGG | + | chr2.4:52791465-52791484 | MS.gene73334:intron | 35.0% |
| !! | AATGAGAGTTGTTTTCTCAG+AGG | + | chr2.4:52791001-52791020 | MS.gene73334:intron | 35.0% |
| AACATGTGTCAGTGTCATGT+TGG | + | chr2.4:52791367-52791386 | MS.gene73334:intron | 40.0% | |
| ATAGAACTGAAACACAGAGC+TGG | - | chr2.4:52790389-52790408 | None:intergenic | 40.0% | |
| ATTCACACTCAAATGTTGGG+TGG | + | chr2.4:52790615-52790634 | MS.gene73334:intron | 40.0% | |
| CAACCATGACATTGAAGATG+GGG | + | chr2.4:52791714-52791733 | MS.gene73334:CDS | 40.0% | |
| CACAACACTCATTGACATGT+AGG | + | chr2.4:52791307-52791326 | MS.gene73334:intron | 40.0% | |
| CAGATACTAGATACTGAGAC+TGG | + | chr2.4:52791785-52791804 | MS.gene73334:CDS | 40.0% | |
| CCATATCTTATTGGAACTGG+GGG | + | chr2.4:52792205-52792224 | MS.gene73334:CDS | 40.0% | |
| CTCAACCATGACATTGAAGA+TGG | + | chr2.4:52791712-52791731 | MS.gene73334:CDS | 40.0% | |
| GAAACATGAAGGAAGAGAAG+TGG | - | chr2.4:52790750-52790769 | None:intergenic | 40.0% | |
| GGGGCTTCATTAAGAAAATG+GGG | + | chr2.4:52790272-52790291 | MS.gene73334:intron | 40.0% | |
| TCTACATTCTCTTCTGTCAG+TGG | - | chr2.4:52791572-52791591 | None:intergenic | 40.0% | |
| TGTTCAACCTCCAAGATTTC+TGG | - | chr2.4:52791767-52791786 | None:intergenic | 40.0% | |
| TTCAATGTCATGGTTGAGCT+AGG | - | chr2.4:52791710-52791729 | None:intergenic | 40.0% | |
| ! | AATTAAGCGTGTCTTGGTGT+CGG | + | chr2.4:52790826-52790845 | MS.gene73334:intron | 40.0% |
| !! | ATATGAGTTTTTGGGCCTAG+AGG | - | chr2.4:52790352-52790371 | None:intergenic | 40.0% |
| !! | GAAGAACCTGCTTTGTTTCT+TGG | - | chr2.4:52791076-52791095 | None:intergenic | 40.0% |
| !! | GATTTGAGGCATTGAGTTGT+AGG | - | chr2.4:52790654-52790673 | None:intergenic | 40.0% |
| !!! | TGCCCCTTATTTTGTTGGAA+AGG | + | chr2.4:52790089-52790108 | MS.gene73334:intron | 40.0% |
| AGGTATGTGCTGCATTGTGT+TGG | + | chr2.4:52789888-52789907 | MS.gene73334:intron | 45.0% | |
| CCAAACACGTCGTCAATCTA+AGG | + | chr2.4:52791416-52791435 | MS.gene73334:intron | 45.0% | |
| CCCCCAGTTCCAATAAGATA+TGG | - | chr2.4:52792208-52792227 | None:intergenic | 45.0% | |
| CCTTAGATTGACGACGTGTT+TGG | - | chr2.4:52791419-52791438 | None:intergenic | 45.0% | |
| GATCCCCATCTTCAATGTCA+TGG | - | chr2.4:52791720-52791739 | None:intergenic | 45.0% | |
| GGTATGTGCTGCATTGTGTT+GGG | + | chr2.4:52789889-52789908 | MS.gene73334:intron | 45.0% | |
| GTGGCATTGCATGAGATAGA+TGG | + | chr2.4:52791641-52791660 | MS.gene73334:CDS | 45.0% | |
| TAAAGTTCGGCTAAGTGGAC+CGG | + | chr2.4:52792267-52792286 | MS.gene73334:CDS | 45.0% | |
| TATCTTATTGGAACTGGGGG+AGG | + | chr2.4:52792208-52792227 | MS.gene73334:CDS | 45.0% | |
| TGCATTGTGTTGGGAGACTA+GGG | + | chr2.4:52789898-52789917 | MS.gene73334:intron | 45.0% | |
| ! | GGTGTCGGATATGTATGGTT+TGG | + | chr2.4:52790841-52790860 | MS.gene73334:intron | 45.0% |
| ! | TGAAGTAAGGCCTGGTTTGA+TGG | + | chr2.4:52791604-52791623 | MS.gene73334:CDS | 45.0% |
| ! | TTGGTGTCTGACATGTGTCA+GGG | - | chr2.4:52791400-52791419 | None:intergenic | 45.0% |
| !! | GTCTTGGTGTCGGATATGTA+TGG | + | chr2.4:52790836-52790855 | MS.gene73334:intron | 45.0% |
| !! | GTTTTGGATGAAGTAAGGCC+TGG | + | chr2.4:52791596-52791615 | MS.gene73334:CDS | 45.0% |
| !! | TTTGGTGTCTGACATGTGTC+AGG | - | chr2.4:52791401-52791420 | None:intergenic | 45.0% |
| !!! | GTTGGTGCCCCTTATTTTGT+TGG | + | chr2.4:52790084-52790103 | MS.gene73334:intron | 45.0% |
| !! | AAAAAAATTAAACAAAATAT+AGG | + | chr2.4:52790251-52790270 | MS.gene73334:intron | 5.0% |
| !! | AAAAAATTAAACAAAATATA+GGG | + | chr2.4:52790252-52790271 | MS.gene73334:intron | 5.0% |
| ACTGTCATAACTCCTGCTGC+CGG | - | chr2.4:52792289-52792308 | None:intergenic | 50.0% | |
| AGCTGCACAGCAGCAATAGA+AGG | - | chr2.4:52792349-52792368 | None:intergenic | 50.0% | |
| CACACGCTCCTTGTAGTTTG+AGG | - | chr2.4:52791679-52791698 | None:intergenic | 50.0% | |
| CATTCTCTTCTGTCAGTGGC+AGG | - | chr2.4:52791568-52791587 | None:intergenic | 50.0% | |
| CGTGCTCCAAGAAACAAAGC+AGG | + | chr2.4:52791067-52791086 | MS.gene73334:intron | 50.0% | |
| CTGCATTGTGTTGGGAGACT+AGG | + | chr2.4:52789897-52789916 | MS.gene73334:intron | 50.0% | |
| GGGTATGTGGTGCATTGAGT+TGG | + | chr2.4:52789918-52789937 | MS.gene73334:intron | 50.0% | |
| TGTTGGGAGACTAGGGTATG+TGG | + | chr2.4:52789905-52789924 | MS.gene73334:intron | 50.0% | |
| ! | CTCAAACTACAAGGAGCGTG+TGG | + | chr2.4:52791677-52791696 | MS.gene73334:CDS | 50.0% |
| ! | CTGGTTTGATGGCTGATGGA+GGG | + | chr2.4:52791615-52791634 | MS.gene73334:CDS | 50.0% |
| CCTCCATCAGCCATCAAACC+AGG | - | chr2.4:52791617-52791636 | None:intergenic | 55.0% | |
| GATGGCTGATGGAGGGAATG+TGG | + | chr2.4:52791622-52791641 | MS.gene73334:CDS | 55.0% | |
| GTCAGTGGCAGGACACATCT+CGG | - | chr2.4:52791557-52791576 | None:intergenic | 55.0% | |
| ! | AGGCCTGGTTTGATGGCTGA+TGG | + | chr2.4:52791611-52791630 | MS.gene73334:CDS | 55.0% |
| ! | CCTGGTTTGATGGCTGATGG+AGG | + | chr2.4:52791614-52791633 | MS.gene73334:CDS | 55.0% |
| CGGCTAAGTGGACCGGCAGC+AGG | + | chr2.4:52792274-52792293 | MS.gene73334:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 52789826 | 52792378 | 52789826 | ID=MS.gene73334 |
| chr2.4 | mRNA | 52789826 | 52792378 | 52789826 | ID=MS.gene73334.t1;Parent=MS.gene73334 |
| chr2.4 | exon | 52789826 | 52789889 | 52789826 | ID=MS.gene73334.t1.exon1;Parent=MS.gene73334.t1 |
| chr2.4 | CDS | 52789826 | 52789889 | 52789826 | ID=cds.MS.gene73334.t1;Parent=MS.gene73334.t1 |
| chr2.4 | exon | 52791540 | 52791838 | 52791540 | ID=MS.gene73334.t1.exon2;Parent=MS.gene73334.t1 |
| chr2.4 | CDS | 52791540 | 52791838 | 52791540 | ID=cds.MS.gene73334.t1;Parent=MS.gene73334.t1 |
| chr2.4 | exon | 52792187 | 52792378 | 52792187 | ID=MS.gene73334.t1.exon3;Parent=MS.gene73334.t1 |
| chr2.4 | CDS | 52792187 | 52792378 | 52792187 | ID=cds.MS.gene73334.t1;Parent=MS.gene73334.t1 |
| Gene Sequence |
| Protein sequence |
