AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062778

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062778.t1 MTR_1g019770 97.784 361 8 0 1 361 1 361 0.0 736
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062778.t1 AT2G37560 66.762 349 110 2 19 361 14 362 2.12e-173 487
MS.gene062778.t1 AT2G37560 64.244 344 109 2 19 361 14 344 2.49e-170 478

Find 71 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAACATGAAAAGGAAAT+TGG 0.153499 1.4:+3480858 MS.gene062778:CDS
AACATGCCTTCAATCTTATA+TGG 0.207992 1.4:-3483452 None:intergenic
AAAGCTAAACGCAAGGTTTC+CGG 0.211000 1.4:+3482332 MS.gene062778:CDS
TATTCTGTAGTTGTCATTAA+TGG 0.229602 1.4:+3482084 MS.gene062778:CDS
TGGAGGCAACAATACGAATA+TGG 0.257115 1.4:-3482523 None:intergenic
GGCAGTTTGGGACAAGAATA+TGG 0.289237 1.4:+3483382 MS.gene062778:intron
ATATTGATGGACCAGGATTA+AGG 0.310552 1.4:+3482456 MS.gene062778:CDS
GATATCAGTAATCTTGTGTT+TGG 0.329500 1.4:-3480700 None:intergenic
CTTAGGGAGAGAAGCCATTC+AGG 0.346202 1.4:-3480912 None:intergenic
ATTCTGTAGTTGTCATTAAT+GGG 0.355542 1.4:+3482085 MS.gene062778:CDS
AGATGAACAACAACGACAAT+TGG 0.357101 1.4:+3480592 None:intergenic
GTAGTAATTCACAATATTGA+TGG 0.374954 1.4:+3482443 MS.gene062778:CDS
GCTCTTCTGATGTATGGCTT+TGG 0.385885 1.4:+3482012 MS.gene062778:CDS
ACATGCCTTCAATCTTATAT+GGG 0.410899 1.4:-3483451 None:intergenic
TCAGACCATTAACTTAAAAC+AGG 0.411589 1.4:+3482113 MS.gene062778:CDS
TTGAATACACTCTGGGCATT+GGG 0.413303 1.4:-3483551 None:intergenic
TACTCTGAATTCTCATTTGA+CGG 0.418075 1.4:+3483755 MS.gene062778:CDS
CCAGACGAGCGAGGTATTGT+TGG 0.423306 1.4:-3482490 None:intergenic
ATATTAGGTTCTGATAGCTT+TGG 0.426600 1.4:+3482286 MS.gene062778:intron
TGAAGTGCTTCGGTTGTAAG+AGG 0.428767 1.4:-3483843 None:intergenic
GGATTCGCTCTTCTGATGTA+TGG 0.431543 1.4:+3482006 MS.gene062778:CDS
TTTGAATACACTCTGGGCAT+TGG 0.433936 1.4:-3483552 None:intergenic
CTAAACATGAAAAGGAAATT+GGG 0.440776 1.4:+3480859 MS.gene062778:CDS
TGGCTCACACACAGTTCAAT+TGG 0.450005 1.4:+3483402 MS.gene062778:CDS
TATTGATGGACCAGGATTAA+GGG 0.455380 1.4:+3482457 MS.gene062778:CDS
CAGACGAGCGAGGTATTGTT+GGG 0.459713 1.4:-3482489 None:intergenic
TTTGCCCCATATAAGATTGA+AGG 0.461148 1.4:+3483446 MS.gene062778:CDS
GTTTCAGAGTCCCTTAATCC+TGG 0.463196 1.4:-3482467 None:intergenic
CCAACAATACCTCGCTCGTC+TGG 0.463209 1.4:+3482490 MS.gene062778:CDS
TATTCAACCTAAACATGAAA+AGG 0.467955 1.4:+3480851 MS.gene062778:CDS
GATGAACAACAACGACAATT+GGG 0.477909 1.4:+3480593 None:intergenic
TCTTACATTGTTGTATGAAG+TGG 0.478921 1.4:+3482391 MS.gene062778:CDS
ATCTTATATGGGGCAAATGT+AGG 0.486995 1.4:-3483440 None:intergenic
CTTGTGTTTGGATTTCTTCA+CGG 0.487363 1.4:-3480688 None:intergenic
GTATGGCTTTGGATCTAAGA+AGG 0.495801 1.4:+3482023 MS.gene062778:CDS
GAGTAACCTGGCTGCTAACA+AGG 0.508129 1.4:-3483737 None:intergenic
TTGACCATCGGAATGCCTCT+TGG 0.512065 1.4:-3483805 None:intergenic
GGAGGCAACAATACGAATAT+GGG 0.514014 1.4:-3482522 None:intergenic
ACGTTTCCTTGTTAGCAGCC+AGG 0.517589 1.4:+3483731 MS.gene062778:CDS
CAAATTTCTCATCCCGATGA+AGG 0.522168 1.4:+3483590 MS.gene062778:CDS
GGGAGCATTAACATGGTCAA+TGG 0.522317 1.4:-3482543 None:intergenic
CTGAATGGCTTCTCTCCCTA+AGG 0.525332 1.4:+3480913 MS.gene062778:CDS
CAAGAACTTTGAATACACTC+TGG 0.530976 1.4:-3483559 None:intergenic
ATCTTCAATAGATTGAGTAC+TGG 0.534306 1.4:-3482366 None:intergenic
TATCGATGTTGTCGATGAGC+AGG 0.537294 1.4:+3480719 MS.gene062778:CDS
TGCTAACAAGGAAACGTTCA+CGG 0.541569 1.4:-3483725 None:intergenic
GGAATTTAAAGATCACGAAC+TGG 0.544269 1.4:+3483776 MS.gene062778:CDS
ATTGAGTACTGGATGGCTGC+CGG 0.547630 1.4:-3482355 None:intergenic
CAACAACGACAATTGGGAAG+AGG 0.569094 1.4:+3480599 MS.gene062778:CDS
AGTACTGGATGGCTGCCGGC+CGG 0.572496 1.4:-3482351 None:intergenic
ACACACAGTTCAATTGGTGC+TGG 0.584222 1.4:+3483408 MS.gene062778:CDS
GAAGTGCTTCGGTTGTAAGA+GGG 0.584506 1.4:-3483842 None:intergenic
ATTCACAATATTGATGGACC+AGG 0.597689 1.4:+3482449 MS.gene062778:CDS
ATGCTGCTATGTATCCTGAA+TGG 0.598898 1.4:+3480898 MS.gene062778:CDS
GTTGTATGAAGTGGAAGTAG+AGG 0.603568 1.4:+3482400 MS.gene062778:CDS
AAGAACTTTGAATACACTCT+GGG 0.606048 1.4:-3483558 None:intergenic
TCAATAGATTGAGTACTGGA+TGG 0.610328 1.4:-3482362 None:intergenic
CATGCCTTCAATCTTATATG+GGG 0.613591 1.4:-3483450 None:intergenic
CTGCCGTGTGCAAGAATCAG+AGG 0.614174 1.4:-3483479 None:intergenic
GTGCAAGCAATCTTGACCAT+CGG 0.614626 1.4:-3483817 None:intergenic
AATACGAATATGGGTACAAG+AGG 0.617434 1.4:-3482513 None:intergenic
AGCATTAACATGGTCAATGG+AGG 0.619176 1.4:-3482540 None:intergenic
TACAAAGGGGAGCATTAACA+TGG 0.630431 1.4:-3482550 None:intergenic
AATGAGAATTCAGAGTAACC+TGG 0.635096 1.4:-3483749 None:intergenic
TCAAATGAAAGCTAAACGCA+AGG 0.651514 1.4:+3482325 MS.gene062778:CDS
TACAAGAGGCCAGACGAGCG+AGG 0.653242 1.4:-3482499 None:intergenic
TATCCTCTGATTCTTGCACA+CGG 0.655621 1.4:+3483476 MS.gene062778:CDS
AAGACCAAGAGGCATTCCGA+TGG 0.661202 1.4:+3483801 MS.gene062778:CDS
CTAAACGCAAGGTTTCCGGC+CGG 0.673224 1.4:+3482336 MS.gene062778:CDS
ACGAACTGGTCAAGACCAAG+AGG 0.697181 1.4:+3483790 MS.gene062778:CDS
ACTGAGTAGAGATCACTGAT+CGG 0.699405 1.4:-3483699 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGATCAATTATTATTAT+TGG - chr1.4:3483113-3483132 None:intergenic 10.0%
!!! TTCAATAACATATTTTAAAA+TGG - chr1.4:3482753-3482772 None:intergenic 10.0%
!!! TTTTTTCTTTTTAACATATT+AGG + chr1.4:3482271-3482290 MS.gene062778:intron 10.0%
!! AATAAACATAGAATTTGAAA+TGG + chr1.4:3481602-3481621 MS.gene062778:intron 15.0%
!! CAAAACTATTAAAAATGATT+TGG + chr1.4:3483181-3483200 MS.gene062778:intron 15.0%
!!! TAAGTTTTTATCATTTTACT+TGG + chr1.4:3483613-3483632 MS.gene062778:intron 15.0%
!!! TTTTATTATGCAAAGAATTA+GGG - chr1.4:3481458-3481477 None:intergenic 15.0%
!! AATGTAATGTGATTTATGTT+TGG + chr1.4:3481079-3481098 MS.gene062778:intron 20.0%
!! TGTACTTAAAATAGCTTATT+TGG - chr1.4:3481835-3481854 None:intergenic 20.0%
!! TTCAAATTCTATGTTTATTG+TGG - chr1.4:3481601-3481620 None:intergenic 20.0%
!!! CTTTTATTATGCAAAGAATT+AGG - chr1.4:3481459-3481478 None:intergenic 20.0%
!!! GATTTTGTAAAATTGATTCT+GGG + chr1.4:3481043-3481062 MS.gene062778:intron 20.0%
!!! TAAATTGTGTGTGAATTTTT+TGG - chr1.4:3481759-3481778 None:intergenic 20.0%
!!! TGATTTTGTAAAATTGATTC+TGG + chr1.4:3481042-3481061 MS.gene062778:intron 20.0%
!!! TTTATTTTCTGTTGAATTTC+TGG + chr1.4:3480771-3480790 MS.gene062778:intron 20.0%
!!! TTTTGATTTTGTATGTGATT+AGG + chr1.4:3480806-3480825 MS.gene062778:intron 20.0%
! AAACATGTAATATAGCTCTT+TGG + chr1.4:3482169-3482188 MS.gene062778:intron 25.0%
! AAGTATATGTGACTATATGA+AGG + chr1.4:3483311-3483330 MS.gene062778:intron 25.0%
! ATTCTGTAGTTGTCATTAAT+GGG + chr1.4:3482085-3482104 MS.gene062778:CDS 25.0%
! CCATATCTAAAGATGAAATA+TGG - chr1.4:3482648-3482667 None:intergenic 25.0%
! CCATATTTCATCTTTAGATA+TGG + chr1.4:3482645-3482664 MS.gene062778:intron 25.0%
! CTAAACATGAAAAGGAAATT+GGG + chr1.4:3480859-3480878 MS.gene062778:CDS 25.0%
! GTAGTAATTCACAATATTGA+TGG + chr1.4:3482443-3482462 MS.gene062778:CDS 25.0%
! TATTCAACCTAAACATGAAA+AGG + chr1.4:3480851-3480870 MS.gene062778:CDS 25.0%
! TATTCTGTAGTTGTCATTAA+TGG + chr1.4:3482084-3482103 MS.gene062778:CDS 25.0%
! TGATAAAAACTTACCTTCAT+CGG - chr1.4:3483606-3483625 None:intergenic 25.0%
! TTATACAGAGCTAAAAACTT+AGG - chr1.4:3482831-3482850 None:intergenic 25.0%
! TTCTTATCATTCAACACTAA+TGG + chr1.4:3480952-3480971 MS.gene062778:intron 25.0%
!! AACATAGAATTTGAAATGGA+TGG + chr1.4:3481606-3481625 MS.gene062778:intron 25.0%
!! ACATAGAATTTGAAATGGAT+GGG + chr1.4:3481607-3481626 MS.gene062778:intron 25.0%
!! TTAACAGTTACATCTTCTTT+TGG + chr1.4:3483361-3483380 MS.gene062778:intron 25.0%
!!! AAGAAACTATTTTCTAGCAA+AGG + chr1.4:3480653-3480672 MS.gene062778:CDS 25.0%
!!! AGAATTGATTTTGACATGTT+TGG - chr1.4:3481133-3481152 None:intergenic 25.0%
!!! ATCTGTTTGAGATTTCTTTT+GGG + chr1.4:3482703-3482722 MS.gene062778:intron 25.0%
!!! CTTGGTTTTGATAGATATAA+TGG + chr1.4:3483233-3483252 MS.gene062778:intron 25.0%
!!! GTGCATATGACATAATTTTT+TGG - chr1.4:3481299-3481318 None:intergenic 25.0%
!!! GTGCATTTTAAGTTTTACAT+TGG + chr1.4:3481943-3481962 MS.gene062778:intron 25.0%
!!! GTTCTAAAGTAAGAAGATAA+AGG - chr1.4:3481555-3481574 None:intergenic 25.0%
!!! TATCTGTTTGAGATTTCTTT+TGG + chr1.4:3482702-3482721 MS.gene062778:intron 25.0%
!!! TATTTTTGCTGTTTATCACT+GGG + chr1.4:3482786-3482805 MS.gene062778:intron 25.0%
!!! TGTGTGTGAATTTTTTGTAA+GGG - chr1.4:3481754-3481773 None:intergenic 25.0%
!!! TTATCTTCTTACTTTAGAAC+CGG + chr1.4:3481555-3481574 MS.gene062778:intron 25.0%
!!! TTATTTTTGCTGTTTATCAC+TGG + chr1.4:3482785-3482804 MS.gene062778:intron 25.0%
!!! TTGTGTGTGAATTTTTTGTA+AGG - chr1.4:3481755-3481774 None:intergenic 25.0%
AACATGCCTTCAATCTTATA+TGG - chr1.4:3483455-3483474 None:intergenic 30.0%
AACTCATATTCGCTTATAAG+TGG + chr1.4:3481357-3481376 MS.gene062778:intron 30.0%
AAGAACTTTGAATACACTCT+GGG - chr1.4:3483561-3483580 None:intergenic 30.0%
AATAGTTAGAATAGCAAGTC+CGG - chr1.4:3481577-3481596 None:intergenic 30.0%
AATTTCTTAGTGAAGGATCT+CGG + chr1.4:3482225-3482244 MS.gene062778:intron 30.0%
ACAGATGAATTTCTTAGTGA+AGG + chr1.4:3482218-3482237 MS.gene062778:intron 30.0%
ACATGCCTTCAATCTTATAT+GGG - chr1.4:3483454-3483473 None:intergenic 30.0%
ACTCATATTCGCTTATAAGT+GGG + chr1.4:3481358-3481377 MS.gene062778:intron 30.0%
AGTGAAAAGTTAAGGCAAAA+TGG + chr1.4:3482193-3482212 MS.gene062778:intron 30.0%
ATCTATCAAAACCAAGACTT+GGG - chr1.4:3483229-3483248 None:intergenic 30.0%
ATCTTCAATAGATTGAGTAC+TGG - chr1.4:3482369-3482388 None:intergenic 30.0%
ATGTGGTCTATAAGATAGTT+TGG + chr1.4:3483071-3483090 MS.gene062778:intron 30.0%
CAGTAACTAAACTTACAAAG+GGG - chr1.4:3482566-3482585 None:intergenic 30.0%
CCTAAACATGAAAAGGAAAT+TGG + chr1.4:3480858-3480877 MS.gene062778:CDS 30.0%
CTCAAGACGAAGAAAATATT+TGG - chr1.4:3482901-3482920 None:intergenic 30.0%
CTGTTAAGAAAAATAGTCAG+AGG - chr1.4:3483348-3483367 None:intergenic 30.0%
GAAAAGAGAGTAATACCTTA+GGG - chr1.4:3480931-3480950 None:intergenic 30.0%
GATAAAAACTTACCTTCATC+GGG - chr1.4:3483605-3483624 None:intergenic 30.0%
GATATCAGTAATCTTGTGTT+TGG - chr1.4:3480703-3480722 None:intergenic 30.0%
GATGGCATATATTAAGCAAA+AGG + chr1.4:3483034-3483053 MS.gene062778:intron 30.0%
GCAGTAACTAAACTTACAAA+GGG - chr1.4:3482567-3482586 None:intergenic 30.0%
TACTCTGAATTCTCATTTGA+CGG + chr1.4:3483755-3483774 MS.gene062778:CDS 30.0%
TATCTATCAAAACCAAGACT+TGG - chr1.4:3483230-3483249 None:intergenic 30.0%
TCAGACCATTAACTTAAAAC+AGG + chr1.4:3482113-3482132 MS.gene062778:CDS 30.0%
TCTTACATTGTTGTATGAAG+TGG + chr1.4:3482391-3482410 MS.gene062778:CDS 30.0%
TGAAAAGAGAGTAATACCTT+AGG - chr1.4:3480932-3480951 None:intergenic 30.0%
! AGCTATATTACATGTTTCTG+TGG - chr1.4:3482166-3482185 None:intergenic 30.0%
! ATATTAGGTTCTGATAGCTT+TGG + chr1.4:3482286-3482305 MS.gene062778:intron 30.0%
! ATGCTGTTCTTTTCATATGT+AGG + chr1.4:3483643-3483662 MS.gene062778:intron 30.0%
! CCAATTTCCTTTTCATGTTT+AGG - chr1.4:3480861-3480880 None:intergenic 30.0%
!! TCTTTGGTAGTGAAAAGTTA+AGG + chr1.4:3482185-3482204 MS.gene062778:intron 30.0%
!!! AGTTTTTAGCTCTGTATAAC+TGG + chr1.4:3482832-3482851 MS.gene062778:intron 30.0%
!!! GCAAACCTGTTTTAAGTTAA+TGG - chr1.4:3482121-3482140 None:intergenic 30.0%
!!! TAGAACTTTTTGAAGTGCTT+CGG - chr1.4:3483856-3483875 None:intergenic 30.0%
AAACGACTCTTGAAATCTTG+TGG + chr1.4:3483272-3483291 MS.gene062778:intron 35.0%
AACTGGACAATAGGACTAAA+TGG + chr1.4:3482849-3482868 MS.gene062778:intron 35.0%
AAGTATGTTATGCGTGTGTT+TGG + chr1.4:3481001-3481020 MS.gene062778:intron 35.0%
AATACGAATATGGGTACAAG+AGG - chr1.4:3482516-3482535 None:intergenic 35.0%
AATGAGAATTCAGAGTAACC+TGG - chr1.4:3483752-3483771 None:intergenic 35.0%
ACAAACACTTACTGCATCTT+TGG - chr1.4:3481268-3481287 None:intergenic 35.0%
ATAGCACATACTTCTTACTC+CGG - chr1.4:3481651-3481670 None:intergenic 35.0%
ATCTTATATGGGGCAAATGT+AGG - chr1.4:3483443-3483462 None:intergenic 35.0%
ATGGATGGGAAATGAAATCA+AGG + chr1.4:3481621-3481640 MS.gene062778:intron 35.0%
ATTAAGCAAAAGGTGGTACT+TGG + chr1.4:3483044-3483063 MS.gene062778:intron 35.0%
ATTTCTTAGTGAAGGATCTC+GGG + chr1.4:3482226-3482245 MS.gene062778:intron 35.0%
CAAGAACTTTGAATACACTC+TGG - chr1.4:3483562-3483581 None:intergenic 35.0%
CATGACTAATCGTTATGTTC+TGG + chr1.4:3481527-3481546 MS.gene062778:intron 35.0%
CATGCCTTCAATCTTATATG+GGG - chr1.4:3483453-3483472 None:intergenic 35.0%
CTTGTGTTTGGATTTCTTCA+CGG - chr1.4:3480691-3480710 None:intergenic 35.0%
GAAATGAAATCAAGGTGAAC+CGG + chr1.4:3481629-3481648 MS.gene062778:intron 35.0%
GCAAGTTGTTAAAGTTGAGA+TGG + chr1.4:3481397-3481416 MS.gene062778:intron 35.0%
GGAAGGGAAAAAATACAGTT+AGG - chr1.4:3483672-3483691 None:intergenic 35.0%
GGAATTTAAAGATCACGAAC+TGG + chr1.4:3483776-3483795 MS.gene062778:CDS 35.0%
GGCAGTAACTAAACTTACAA+AGG - chr1.4:3482568-3482587 None:intergenic 35.0%
GGCATATATTAAGCAAAAGG+TGG + chr1.4:3483037-3483056 MS.gene062778:intron 35.0%
GGCTCATTCAAAAAAAGTGA+AGG - chr1.4:3481980-3481999 None:intergenic 35.0%
GTCTATAAGATAGTTTGGTC+AGG + chr1.4:3483076-3483095 MS.gene062778:intron 35.0%
TAACAACTTGCAATGCTAGA+GGG - chr1.4:3481388-3481407 None:intergenic 35.0%
TCAAATGAAAGCTAAACGCA+AGG + chr1.4:3482325-3482344 MS.gene062778:CDS 35.0%
TCTGCTTTACAGTTGTTTGT+AGG + chr1.4:3482955-3482974 MS.gene062778:intron 35.0%
TGACTACATTTGTGAAAGAC+AGG + chr1.4:3483005-3483024 MS.gene062778:intron 35.0%
TTAACAACTTGCAATGCTAG+AGG - chr1.4:3481389-3481408 None:intergenic 35.0%
TTTGCCCCATATAAGATTGA+AGG + chr1.4:3483446-3483465 MS.gene062778:CDS 35.0%
! ACATCTTCTTTTGGCAGTTT+GGG + chr1.4:3483370-3483389 MS.gene062778:intron 35.0%
! AGAAGTATGTGCTATGTAAG+CGG + chr1.4:3481656-3481675 MS.gene062778:intron 35.0%
! ATATTGATGGACCAGGATTA+AGG + chr1.4:3482456-3482475 MS.gene062778:CDS 35.0%
! ATTCACAATATTGATGGACC+AGG + chr1.4:3482449-3482468 MS.gene062778:CDS 35.0%
! GGTTCTACAGCTTTCTAAAA+GGG - chr1.4:3482880-3482899 None:intergenic 35.0%
! TACATCTTCTTTTGGCAGTT+TGG + chr1.4:3483369-3483388 MS.gene062778:intron 35.0%
! TATTGATGGACCAGGATTAA+GGG + chr1.4:3482457-3482476 MS.gene062778:CDS 35.0%
! TGGTTCTACAGCTTTCTAAA+AGG - chr1.4:3482881-3482900 None:intergenic 35.0%
!! TCAATAGATTGAGTACTGGA+TGG - chr1.4:3482365-3482384 None:intergenic 35.0%
!!! AACTGTATTTTTTCCCTTCC+AGG + chr1.4:3483672-3483691 MS.gene062778:intron 35.0%
!!! AATTTTGACTCCTCCAAAAG+TGG + chr1.4:3481240-3481259 MS.gene062778:intron 35.0%
!!! TTTTTTGAATGAGCCAGATG+CGG + chr1.4:3481985-3482004 MS.gene062778:intron 35.0%
AAAACCAAGACTTGGGACTT+GGG - chr1.4:3483222-3483241 None:intergenic 40.0%
AAAGCTAAACGCAAGGTTTC+CGG + chr1.4:3482332-3482351 MS.gene062778:CDS 40.0%
ACTGAGTAGAGATCACTGAT+CGG - chr1.4:3483702-3483721 None:intergenic 40.0%
AGAGGAAGAAGAAGAAGAAG+AGG + chr1.4:3480617-3480636 MS.gene062778:CDS 40.0%
AGCATTAACATGGTCAATGG+AGG - chr1.4:3482543-3482562 None:intergenic 40.0%
ATGCTGCTATGTATCCTGAA+TGG + chr1.4:3480898-3480917 MS.gene062778:CDS 40.0%
CAAATTTCTCATCCCGATGA+AGG + chr1.4:3483590-3483609 MS.gene062778:CDS 40.0%
CTACGTCAATGATAGCAAAG+TGG - chr1.4:3481490-3481509 None:intergenic 40.0%
CTGTGGAATGCAAGATGTTA+GGG - chr1.4:3482149-3482168 None:intergenic 40.0%
CTTGAAATCTTGTGGCAGTA+GGG + chr1.4:3483280-3483299 MS.gene062778:intron 40.0%
GCTCTGTATAACTGGACAAT+AGG + chr1.4:3482840-3482859 MS.gene062778:intron 40.0%
GGAGGCAACAATACGAATAT+GGG - chr1.4:3482525-3482544 None:intergenic 40.0%
GGGTGCTTGAACTTTGAATT+GGG + chr1.4:3482723-3482742 MS.gene062778:intron 40.0%
GTTGTATGAAGTGGAAGTAG+AGG + chr1.4:3482400-3482419 MS.gene062778:CDS 40.0%
TATCATTGACGTAGGCATAC+TGG + chr1.4:3481496-3481515 MS.gene062778:intron 40.0%
TATCCTCTGATTCTTGCACA+CGG + chr1.4:3483476-3483495 MS.gene062778:CDS 40.0%
TCTGTGGAATGCAAGATGTT+AGG - chr1.4:3482150-3482169 None:intergenic 40.0%
TCTTGAAATCTTGTGGCAGT+AGG + chr1.4:3483279-3483298 MS.gene062778:intron 40.0%
TGCTAACAAGGAAACGTTCA+CGG - chr1.4:3483728-3483747 None:intergenic 40.0%
TGGAGGCAACAATACGAATA+TGG - chr1.4:3482526-3482545 None:intergenic 40.0%
TGGGTGCTTGAACTTTGAAT+TGG + chr1.4:3482722-3482741 MS.gene062778:intron 40.0%
TTCTGCACGAGATAATCTGA+CGG - chr1.4:3482589-3482608 None:intergenic 40.0%
TTGAATACACTCTGGGCATT+GGG - chr1.4:3483554-3483573 None:intergenic 40.0%
TTTGAATACACTCTGGGCAT+TGG - chr1.4:3483555-3483574 None:intergenic 40.0%
! CACTTTGCTATCATTGACGT+AGG + chr1.4:3481488-3481507 MS.gene062778:intron 40.0%
! GCTTGCAGAATTGATTCTAG+AGG - chr1.4:3481159-3481178 None:intergenic 40.0%
! TGGTGTATCTAGAGTGTGTT+TGG + chr1.4:3480972-3480991 MS.gene062778:intron 40.0%
!! ATCTTTGGTTCCACTTTTGG+AGG - chr1.4:3481253-3481272 None:intergenic 40.0%
!! GTATGGCTTTGGATCTAAGA+AGG + chr1.4:3482023-3482042 MS.gene062778:CDS 40.0%
!! TACAAAGGGGAGCATTAACA+TGG - chr1.4:3482553-3482572 None:intergenic 40.0%
!! TGCATCTTTGGTTCCACTTT+TGG - chr1.4:3481256-3481275 None:intergenic 40.0%
!!! TTGCAGCTGTTTTGACACTT+TGG - chr1.4:3483511-3483530 None:intergenic 40.0%
ACACACAGTTCAATTGGTGC+TGG + chr1.4:3483408-3483427 MS.gene062778:CDS 45.0%
AGGTGGTACTTGGTAGAATG+TGG + chr1.4:3483054-3483073 MS.gene062778:intron 45.0%
CAAAACCAAGACTTGGGACT+TGG - chr1.4:3483223-3483242 None:intergenic 45.0%
CAACAACGACAATTGGGAAG+AGG + chr1.4:3480599-3480618 MS.gene062778:CDS 45.0%
GCTCTTCTGATGTATGGCTT+TGG + chr1.4:3482012-3482031 MS.gene062778:CDS 45.0%
GGATTCGCTCTTCTGATGTA+TGG + chr1.4:3482006-3482025 MS.gene062778:CDS 45.0%
GTGCAAGCAATCTTGACCAT+CGG - chr1.4:3483820-3483839 None:intergenic 45.0%
GTTTCAGAGTCCCTTAATCC+TGG - chr1.4:3482470-3482489 None:intergenic 45.0%
TATCGATGTTGTCGATGAGC+AGG + chr1.4:3480719-3480738 MS.gene062778:CDS 45.0%
TGGCTCACACACAGTTCAAT+TGG + chr1.4:3483402-3483421 MS.gene062778:CDS 45.0%
TTATCACTGGGTGTTCTCTG+AGG + chr1.4:3482798-3482817 MS.gene062778:intron 45.0%
! GAAGTGCTTCGGTTGTAAGA+GGG - chr1.4:3483845-3483864 None:intergenic 45.0%
! GGCAGTTTGGGACAAGAATA+TGG + chr1.4:3483382-3483401 MS.gene062778:intron 45.0%
! GTGAAAGACAGGAAGTACGA+TGG + chr1.4:3483016-3483035 MS.gene062778:intron 45.0%
! TGAAGTGCTTCGGTTGTAAG+AGG - chr1.4:3483846-3483865 None:intergenic 45.0%
!! GGGAGCATTAACATGGTCAA+TGG - chr1.4:3482546-3482565 None:intergenic 45.0%
!!! AGCTGTTTTGACACTTTGGC+TGG - chr1.4:3483507-3483526 None:intergenic 45.0%
ACGAACTGGTCAAGACCAAG+AGG + chr1.4:3483790-3483809 MS.gene062778:CDS 50.0%
ACGTTTCCTTGTTAGCAGCC+AGG + chr1.4:3483731-3483750 MS.gene062778:CDS 50.0%
ACTGATCGGCATTCCTGGAA+GGG - chr1.4:3483688-3483707 None:intergenic 50.0%
AGATCACTGATCGGCATTCC+TGG - chr1.4:3483693-3483712 None:intergenic 50.0%
CTGAATGGCTTCTCTCCCTA+AGG + chr1.4:3480913-3480932 MS.gene062778:CDS 50.0%
CTTAGGGAGAGAAGCCATTC+AGG - chr1.4:3480915-3480934 None:intergenic 50.0%
GAGTAACCTGGCTGCTAACA+AGG - chr1.4:3483740-3483759 None:intergenic 50.0%
TTGACCATCGGAATGCCTCT+TGG - chr1.4:3483808-3483827 None:intergenic 50.0%
!! AAGACCAAGAGGCATTCCGA+TGG + chr1.4:3483801-3483820 MS.gene062778:CDS 50.0%
!! ATTGAGTACTGGATGGCTGC+CGG - chr1.4:3482358-3482377 None:intergenic 50.0%
!! CAGACGAGCGAGGTATTGTT+GGG - chr1.4:3482492-3482511 None:intergenic 50.0%
CACTGATCGGCATTCCTGGA+AGG - chr1.4:3483689-3483708 None:intergenic 55.0%
CAGAAGAGCGAATCCGCATC+TGG - chr1.4:3482001-3482020 None:intergenic 55.0%
CCAACAATACCTCGCTCGTC+TGG + chr1.4:3482490-3482509 MS.gene062778:CDS 55.0%
CTAAACGCAAGGTTTCCGGC+CGG + chr1.4:3482336-3482355 MS.gene062778:CDS 55.0%
CTGCCGTGTGCAAGAATCAG+AGG - chr1.4:3483482-3483501 None:intergenic 55.0%
TGCTCCCAAGTCCCAAGTCT+TGG + chr1.4:3483215-3483234 MS.gene062778:intron 55.0%
!! CCAGACGAGCGAGGTATTGT+TGG - chr1.4:3482493-3482512 None:intergenic 55.0%
TACAAGAGGCCAGACGAGCG+AGG - chr1.4:3482502-3482521 None:intergenic 60.0%
! AGTACTGGATGGCTGCCGGC+CGG - chr1.4:3482354-3482373 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 3480594 3483890 3480594 ID=MS.gene062778
chr1.4 mRNA 3480594 3483890 3480594 ID=MS.gene062778.t1;Parent=MS.gene062778
chr1.4 exon 3480594 3480740 3480594 ID=MS.gene062778.t1.exon1;Parent=MS.gene062778.t1
chr1.4 CDS 3480594 3480740 3480594 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
chr1.4 exon 3480828 3480934 3480828 ID=MS.gene062778.t1.exon2;Parent=MS.gene062778.t1
chr1.4 CDS 3480828 3480934 3480828 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
chr1.4 exon 3482002 3482134 3482002 ID=MS.gene062778.t1.exon3;Parent=MS.gene062778.t1
chr1.4 CDS 3482002 3482134 3482002 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
chr1.4 exon 3482293 3482569 3482293 ID=MS.gene062778.t1.exon4;Parent=MS.gene062778.t1
chr1.4 CDS 3482293 3482569 3482293 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
chr1.4 exon 3483387 3483611 3483387 ID=MS.gene062778.t1.exon5;Parent=MS.gene062778.t1
chr1.4 CDS 3483387 3483611 3483387 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
chr1.4 exon 3483694 3483890 3483694 ID=MS.gene062778.t1.exon6;Parent=MS.gene062778.t1
chr1.4 CDS 3483694 3483890 3483694 ID=cds.MS.gene062778.t1;Parent=MS.gene062778.t1
Gene Sequence

>MS.gene062778

ATGAACAACAACGACAATTGGGAAGAGGAAGAAGAAGAAGAAGAGGATCTAGAATTCTCAAGAAACTATTTTCTAGCAAAGGAATTAACATCTTCCGTGAAGAAATCCAAACACAAGATTACTGATATCGATGTTGTCGATGAGCAGGTAAGTATCAATTGATGAATAAAAATGAACTTTATTTTCTGTTGAATTTCTGGTTAAACTTTAGTTTTTGATTTTGTATGTGATTAGGAACTTAGAGAAGCTTCATTGCATATTCAACCTAAACATGAAAAGGAAATTGGGCTTTTAATCGATTCGTATGCTGCTATGTATCCTGAATGGCTTCTCTCCCTAAGGTATTACTCTCTTTTCATTCTTATCATTCAACACTAATGGTGTATCTAGAGTGTGTTTGGAGTGAGAAGTATGTTATGCGTGTGTTTGGTTATGCGTTAACGTAAATTGATTTTGTAAAATTGATTCTGGGTAAAAGTGAGTTGAATGTAATGTGATTTATGTTTGGATGTATTGATGTTAAAGTGAAAAATTAACCAAACATGTCAAAATCAATTCTACACCTCTAGAATCAATTCTGCAAGCGAAATCAATTCTTCTTTAAATCAACCAAACATGTCAAAATCAATTCTACGTCTCTAGAATCAATTTTGACTCCTCCAAAAGTGGAACCAAAGATGCAGTAAGTGTTTGTTTAACATACCAAAAAATTATGTCATATGCACTTGAATGTTTGTTCGTAATACTTGTTTCAATTGAGTATAACTCATATTCGCTTATAAGTGGGCAAACCCTCTAGCATTGCAAGTTGTTAAAGTTGAGATGGACAGTAGTATTTAAATAAAGCTTTGTACTTTGTAACCCTAATTCTTTGCATAATAAAAGTGCAATAGCCACTTTGCTATCATTGACGTAGGCATACTGGCTGAACTTCATGACTAATCGTTATGTTCTGGTGCCTTTATCTTCTTACTTTAGAACCGGACTTGCTATTCTAACTATTTCCACAATAAACATAGAATTTGAAATGGATGGGAAATGAAATCAAGGTGAACCGGAGTAAGAAGTATGTGCTATGTAAGCGGTCTTCATAAGCTATCTGAGAATCTGTTAAAATAAATTATAAACAACTTATGTATATAAACTATTTTTGTATACCCTTACAAAAAATTCACACACAATTTATATCATGAGATATGTCCAAAAAATTCACACACAATTTATATCATGAGATATGTCCAAATAAGCTATTTTAAGTACAATTTTATACGCTTTCATTGCTGTATAATGTATTAATGTATTATAGCTTGTTGAATTTGATTTGTATTTTTAAAAAGTGACTTTAAGATGTGCATTTTAAGTTTTACATTGGAATTATTGTGACCTTCACTTTTTTTGAATGAGCCAGATGCGGATTCGCTCTTCTGATGTATGGCTTTGGATCTAAGAAGGCTTTGATTGAAGACTTTGCTTCGAAGACATTGACAGAATATTCTGTAGTTGTCATTAATGGGTATCTTCAGACCATTAACTTAAAACAGGTTTGCATTCTCCCTAACATCTTGCATTCCACAGAAACATGTAATATAGCTCTTTGGTAGTGAAAAGTTAAGGCAAAATGGAGACAGATGAATTTCTTAGTGAAGGATCTCGGGTTTTCGATGCTCTAAGCTTTAGTTTTTTCTTTTTAACATATTAGGTTCTGATAGCTTTGGCTGAACTTCTATGTGATCAAATGAAAGCTAAACGCAAGGTTTCCGGCCGGCAGCCATCCAGTACTCAATCTATTGAAGATCTTCTTACATTGTTGTATGAAGTGGAAGTAGAGGACAGCAATTCTTTCGTTTGTGTAGTAATTCACAATATTGATGGACCAGGATTAAGGGACTCTGAAACCCAACAATACCTCGCTCGTCTGGCCTCTTGTACCCATATTCGTATTGTTGCCTCCATTGACCATGTTAATGCTCCCCTTTGTAAGTTTAGTTACTGCCGTCAGATTATCTCGTGCAGAATATATTTCTTGTAAATTGTTAGTGTAGTCAATATTGCCATATTTCATCTTTAGATATGGCTTGAAGTTATGTTTTCTTGAATTGTTCTATTATTATCTGTTTGAGATTTCTTTTGGGTGCTTGAACTTTGAATTGGGAATGCCATTTTAAAATATGTTATTGAATTTATTTATTTATTATTTTTGCTGTTTATCACTGGGTGTTCTCTGAGGATCTGATCCTAAGTTTTTAGCTCTGTATAACTGGACAATAGGACTAAATGGCATCACCCTTTTAGAAAGCTGTAGAACCAAATATTTTCTTCGTCTTGAGACTTAAAGTTGCTATCATATTATTTATTTGAACTTCTGCTTTACAGTTGTTTGTAGGAAAGAAATGAATATATAAGATCAGAACTGACTACATTTGTGAAAGACAGGAAGTACGATGGCATATATTAAGCAAAAGGTGGTACTTGGTAGAATGTGGTCTATAAGATAGTTTGGTCAGGCGAGTAAAAGACCAATAATAATAATTGATCAAATGCGAAGTAGCTTACAATGTCAGATGCTGAATATGATTAAGAAAAACAGCAAAACTATTAAAAATGATTTGGATCTAAATGCTTGCTCCCAAGTCCCAAGTCTTGGTTTTGATAGATATAATGGCATCATTTAATCAGAGAAACGACTCTTGAAATCTTGTGGCAGTAGGGTATAAAATAAGTATATGTGACTATATGAAGGTTTTTTTGTTGCCTCTGACTATTTTTCTTAACAGTTACATCTTCTTTTGGCAGTTTGGGACAAGAATATGGCTCACACACAGTTCAATTGGTGCTGGCATCATGTTCCTACATTTGCCCCATATAAGATTGAAGGCATGTTTTATCCTCTGATTCTTGCACACGGCAGTGCCAGCCAAAGTGTCAAAACAGCTGCAATAGTTTTGCAAAGTTTGACACCCAATGCCCAGAGTGTATTCAAAGTTCTTGCTGAATATCAAATTTCTCATCCCGATGAAGGTAAGTTTTTATCATTTTACTTGGATATCATATGCTGTTCTTTTCATATGTAGGTGTCCTAACTGTATTTTTTCCCTTCCAGGAATGCCGATCAGTGATCTCTACTCAGTCTGCCGTGAACGTTTCCTTGTTAGCAGCCAGGTTACTCTGAATTCTCATTTGACGGAATTTAAAGATCACGAACTGGTCAAGACCAAGAGGCATTCCGATGGTCAAGATTGCTTGCACATCCCTCTTACAACCGAAGCACTTCAAAAAGTTCTACTTGAGATCAGTTAG
Protein sequence

>MS.gene062778.t1

MNNNDNWEEEEEEEEDLEFSRNYFLAKELTSSVKKSKHKITDIDVVDEQELREASLHIQPKHEKEIGLLIDSYAAMYPEWLLSLRCGFALLMYGFGSKKALIEDFASKTLTEYSVVVINGYLQTINLKQVLIALAELLCDQMKAKRKVSGRQPSSTQSIEDLLTLLYEVEVEDSNSFVCVVIHNIDGPGLRDSETQQYLARLASCTHIRIVASIDHVNAPLFWDKNMAHTQFNWCWHHVPTFAPYKIEGMFYPLILAHGSASQSVKTAAIVLQSLTPNAQSVFKVLAEYQISHPDEGMPISDLYSVCRERFLVSSQVTLNSHLTEFKDHELVKTKRHSDGQDCLHIPLTTEALQKVLLEIS