AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066535


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066535.t1 MTR_7g114060 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene066535.t1 MTR_7g114040 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene066535.t1 MTR_7g108320 95.775 142 3 1 1 139 1 142 4.40e-93 265
MS.gene066535.t1 MTR_7g093910 87.313 134 17 0 6 139 9 142 6.22e-82 237
MS.gene066535.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 3.86e-72 212
MS.gene066535.t1 MTR_2g082510 86.777 121 12 1 10 126 3 123 3.42e-69 204
MS.gene066535.t1 MTR_2g082220 84.921 126 11 2 10 129 3 126 4.30e-69 204
MS.gene066535.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 1.15e-59 181
MS.gene066535.t1 MTR_7g113160 76.068 117 28 0 9 125 16 132 3.78e-59 179
MS.gene066535.t1 MTR_4g063280 79.091 110 23 0 18 127 17 126 2.93e-58 177
MS.gene066535.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 5.36e-58 177
MS.gene066535.t1 MTR_4g063410 79.439 107 22 0 19 125 24 130 5.44e-58 177
MS.gene066535.t1 MTR_4g071150 80.374 107 21 0 19 125 24 130 6.46e-58 177
MS.gene066535.t1 MTR_4g064005 81.132 106 20 0 19 124 24 129 6.49e-58 177
MS.gene066535.t1 MTR_8g086640 81.132 106 20 0 19 124 24 129 7.30e-58 177
MS.gene066535.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 8.16e-58 176
MS.gene066535.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 5.87e-42 136
MS.gene066535.t1 MTR_1g011750 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene066535.t1 MTR_1g011850 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene066535.t1 MTR_1g114050 62.857 105 37 2 20 123 24 127 5.35e-38 125
MS.gene066535.t1 MTR_1g071040 63.462 104 36 2 21 123 28 130 1.15e-37 125
MS.gene066535.t1 MTR_4g063300 67.708 96 23 1 40 127 10 105 7.05e-36 120
MS.gene066535.t1 MTR_4g005910 88.889 45 5 0 82 126 4 48 6.44e-23 85.5
MS.gene066535.t1 MTR_7g010440 54.444 90 41 0 47 136 96 185 2.92e-22 89.7
MS.gene066535.t1 MTR_4g048490 44.860 107 30 2 19 125 26 103 2.83e-20 80.1
MS.gene066535.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 1.19e-18 75.9
MS.gene066535.t1 MTR_7g027610 41.818 110 32 3 18 123 13 94 8.02e-16 68.9
MS.gene066535.t1 MTR_0653s0030 52.830 53 25 0 50 102 43 95 4.63e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066535.t1 AT1G54690 92.958 142 7 1 1 139 1 142 2.22e-90 258
MS.gene066535.t1 AT1G08880 94.118 136 8 0 4 139 7 142 5.41e-90 258
MS.gene066535.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene066535.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene066535.t1 AT5G54640 82.946 129 19 1 1 129 1 126 1.59e-69 205
MS.gene066535.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.51e-68 203
MS.gene066535.t1 AT3G20670 84.677 124 15 1 10 129 3 126 4.76e-68 202
MS.gene066535.t1 AT5G59870 73.643 129 29 2 1 124 1 129 1.24e-60 184
MS.gene066535.t1 AT5G27670 73.438 128 29 1 2 124 3 130 4.78e-58 177
MS.gene066535.t1 AT5G02560 79.439 107 22 0 18 124 23 129 5.23e-57 174
MS.gene066535.t1 AT5G02560 64.885 131 22 1 18 124 23 153 1.37e-51 162
MS.gene066535.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene066535.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene066535.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene066535.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene066535.t1 AT1G52740 61.905 105 38 2 20 123 27 130 2.42e-38 127
MS.gene066535.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene066535.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene066535.t1 AT4G13570 43.220 118 65 2 1 117 2 118 3.98e-23 87.8
MS.gene066535.t1 AT4G13570 49.451 91 44 2 28 117 35 124 9.39e-22 84.3

Find 49 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCCTTTCCAACCTTCTT+AGG 0.222545 7.1:+2487845 None:intergenic
ATCTGCTTCTCAAGAATTCT+AGG 0.264115 7.1:-2487810 None:intergenic
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.1:-2487924 MS.gene066535:CDS
GAAACTGATTTGGCAGCCTT+TGG 0.309618 7.1:+2488269 None:intergenic
TGAAGATCTTGAAACTGATT+TGG 0.311823 7.1:+2488259 None:intergenic
AACACGTTCGGCGTATTTAC+CGG 0.321195 7.1:+2488187 None:intergenic
GGAAAGGGCAAAGGAGAAAT+TGG 0.326882 7.1:-2487833 MS.gene066535:CDS
AGCAAAGTCTGATGAATATT+AGG 0.347792 7.1:+2487869 None:intergenic
TCAGACTTTGCTACCTAAGA+AGG 0.421667 7.1:-2487858 MS.gene066535:CDS
GGCACATTCAGCTTGCTGTT+AGG 0.440630 7.1:-2487952 MS.gene066535:CDS
GAGTTCCACTGGCACAACTA+AGG 0.456467 7.1:-2488306 MS.gene066535:CDS
AGGACGGCGGAGAGGTAGAC+AGG 0.463287 7.1:+2488152 None:intergenic
GGGACTGTGACCATTGCTAA+TGG 0.468303 7.1:-2487902 MS.gene066535:CDS
AGCACCGGCACCAACACGTT+CGG 0.480590 7.1:+2488175 None:intergenic
GTTTCAAGATCTTCAAAGGC+AGG 0.496108 7.1:-2488251 MS.gene066535:CDS
CTGGCACAACTAAGGGCGGA+AGG 0.502885 7.1:-2488298 MS.gene066535:CDS
ATCAGTTTCAAGATCTTCAA+AGG 0.503488 7.1:-2488255 MS.gene066535:CDS
CTACCTAAGAAGGTTGGAAA+GGG 0.510822 7.1:-2487848 MS.gene066535:CDS
TCTGCTTCTCAAGAATTCTA+GGG 0.519604 7.1:-2487809 None:intergenic
GGCACAACTAAGGGCGGAAG+GGG 0.525782 7.1:-2488296 MS.gene066535:CDS
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7.1:-2488003 MS.gene066535:CDS
ACTTTGCTACCTAAGAAGGT+TGG 0.535784 7.1:-2487854 MS.gene066535:CDS
TGGCACAACTAAGGGCGGAA+GGG 0.543224 7.1:-2488297 MS.gene066535:CDS
AGTTCCACTGGCACAACTAA+GGG 0.549244 7.1:-2488305 MS.gene066535:CDS
TACGCCGAACGTGTTGGTGC+CGG 0.556200 7.1:-2488179 MS.gene066535:CDS
GCTACCTAAGAAGGTTGGAA+AGG 0.557785 7.1:-2487849 MS.gene066535:CDS
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7.1:-2488002 MS.gene066535:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.570928 7.1:+2487956 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7.1:+2487966 None:intergenic
GGGCGGAAGGGGTAAGCCAA+AGG 0.573881 7.1:-2488285 MS.gene066535:CDS
CGGCGTATTTACCGGCCTTG+AGG 0.577173 7.1:+2488195 None:intergenic
ATCGCGAGATTCCTCAAGGC+CGG 0.578871 7.1:-2488206 MS.gene066535:CDS
TCCGCCCTTAGTTGTGCCAG+TGG 0.580065 7.1:+2488301 None:intergenic
GAATCTCGCGATTCTTCCGA+CGG 0.592664 7.1:+2488217 None:intergenic
GGTAAATACGCCGAACGTGT+TGG 0.593895 7.1:-2488185 MS.gene066535:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.612329 7.1:-2488132 MS.gene066535:intron
AAGAAGGTTGGAAAGGGCAA+AGG 0.621113 7.1:-2487842 MS.gene066535:CDS
AATCTCGCGATTCTTCCGAC+GGG 0.626786 7.1:+2488218 None:intergenic
TAAAACACCACCATTAGCAA+TGG 0.630364 7.1:+2487892 None:intergenic
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.1:+2488132 None:intergenic
GGAGAGGTAGACAGGAGCAC+CGG 0.644805 7.1:+2488160 None:intergenic
TCCACTGGCACAACTAAGGG+CGG 0.647888 7.1:-2488302 MS.gene066535:CDS
AAGAATCGCGAGATTCCTCA+AGG 0.651972 7.1:-2488210 MS.gene066535:CDS
ACTGTGACCATTGCTAATGG+TGG 0.653058 7.1:-2487899 MS.gene066535:CDS
GCAGGTCTTCAATTTCCCGT+CGG 0.653866 7.1:-2488233 MS.gene066535:CDS
GATACTCAAGGACGGCGGAG+AGG 0.669935 7.1:+2488144 None:intergenic
AGCAGCGAGATACTCAAGGA+CGG 0.684917 7.1:+2488136 None:intergenic
AGAAGAACCGTATTGTACCA+AGG 0.686303 7.1:-2487973 MS.gene066535:CDS
AGCGAGATACTCAAGGACGG+CGG 0.736224 7.1:+2488139 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAGTTTAATCAAAT+TGG + chr7.1:2488074-2488093 None:intergenic 15.0%
!!! ATTAAACTGTATTTTCATTT+TGG - chr7.1:2488079-2488098 MS.gene066535:intron 15.0%
!!! GTATTTTCATTTTGGTATTT+AGG - chr7.1:2488087-2488106 MS.gene066535:intron 20.0%
!!! TCTCTTTTTTGTATAATGTA+GGG - chr7.1:2488040-2488059 MS.gene066535:intron 20.0%
! ATACAAAAAAGAGAGTGAAA+GGG + chr7.1:2488034-2488053 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG + chr7.1:2488035-2488054 None:intergenic 25.0%
!!! CTCTCTTTTTTGTATAATGT+AGG - chr7.1:2488039-2488058 MS.gene066535:intron 25.0%
!!! TCATTTTGGTATTTAGGTTT+TGG - chr7.1:2488093-2488112 MS.gene066535:intron 25.0%
AGCAAAGTCTGATGAATATT+AGG + chr7.1:2488253-2488272 None:intergenic 30.0%
ATCAGTTTCAAGATCTTCAA+AGG - chr7.1:2487864-2487883 MS.gene066535:CDS 30.0%
TGAAGATCTTGAAACTGATT+TGG + chr7.1:2487863-2487882 None:intergenic 30.0%
!!! TGGTATTTAGGTTTTGGAAT+TGG - chr7.1:2488099-2488118 MS.gene066535:intron 30.0%
GTGAAAGGGAAATTGAAGAA+AGG + chr7.1:2488020-2488039 None:intergenic 35.0%
TAAAACACCACCATTAGCAA+TGG + chr7.1:2488230-2488249 None:intergenic 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG - chr7.1:2488103-2488122 MS.gene066535:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG - chr7.1:2488104-2488123 MS.gene066535:intron 35.0%
ACTTTGCTACCTAAGAAGGT+TGG - chr7.1:2488265-2488284 MS.gene066535:CDS 40.0%
AGAAGAACCGTATTGTACCA+AGG - chr7.1:2488146-2488165 MS.gene066535:CDS 40.0%
CTACCTAAGAAGGTTGGAAA+GGG - chr7.1:2488271-2488290 MS.gene066535:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG + chr7.1:2488156-2488175 None:intergenic 40.0%
GTTTCAAGATCTTCAAAGGC+AGG - chr7.1:2487868-2487887 MS.gene066535:CDS 40.0%
TCAGACTTTGCTACCTAAGA+AGG - chr7.1:2488261-2488280 MS.gene066535:CDS 40.0%
! AAGAACTCAGCAAGCTTTTG+GGG - chr7.1:2488197-2488216 MS.gene066535:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG - chr7.1:2488196-2488215 MS.gene066535:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG - chr7.1:2488195-2488214 MS.gene066535:CDS 40.0%
AACACGTTCGGCGTATTTAC+CGG + chr7.1:2487935-2487954 None:intergenic 45.0%
AAGAAGGTTGGAAAGGGCAA+AGG - chr7.1:2488277-2488296 MS.gene066535:CDS 45.0%
AAGAATCGCGAGATTCCTCA+AGG - chr7.1:2487909-2487928 MS.gene066535:CDS 45.0%
ACTGTGACCATTGCTAATGG+TGG - chr7.1:2488220-2488239 MS.gene066535:CDS 45.0%
AGTTCCACTGGCACAACTAA+GGG - chr7.1:2487814-2487833 MS.gene066535:CDS 45.0%
GCTACCTAAGAAGGTTGGAA+AGG - chr7.1:2488270-2488289 MS.gene066535:CDS 45.0%
GGAAAGGGCAAAGGAGAAAT+TGG - chr7.1:2488286-2488305 MS.gene066535:CDS 45.0%
TTGCCCTTTCCAACCTTCTT+AGG + chr7.1:2488277-2488296 None:intergenic 45.0%
!! GAAACTGATTTGGCAGCCTT+TGG + chr7.1:2487853-2487872 None:intergenic 45.0%
AATCTCGCGATTCTTCCGAC+GGG + chr7.1:2487904-2487923 None:intergenic 50.0%
ACAGCAAGCTGAATGTGCCT+TGG + chr7.1:2488166-2488185 None:intergenic 50.0%
AGCAGCGAGATACTCAAGGA+CGG + chr7.1:2487986-2488005 None:intergenic 50.0%
GAATCTCGCGATTCTTCCGA+CGG + chr7.1:2487905-2487924 None:intergenic 50.0%
GAGTTCCACTGGCACAACTA+AGG - chr7.1:2487813-2487832 MS.gene066535:CDS 50.0%
GCAGGTCTTCAATTTCCCGT+CGG - chr7.1:2487886-2487905 MS.gene066535:CDS 50.0%
GGGACTGTGACCATTGCTAA+TGG - chr7.1:2488217-2488236 MS.gene066535:CDS 50.0%
GGTAAATACGCCGAACGTGT+TGG - chr7.1:2487934-2487953 MS.gene066535:CDS 50.0%
!! GGCACATTCAGCTTGCTGTT+AGG - chr7.1:2488167-2488186 MS.gene066535:CDS 50.0%
AATTGGCTGGGAACGCTGCA+AGG - chr7.1:2488116-2488135 MS.gene066535:intron 55.0%
AGCGAGATACTCAAGGACGG+CGG + chr7.1:2487983-2488002 None:intergenic 55.0%
ATCGCGAGATTCCTCAAGGC+CGG - chr7.1:2487913-2487932 MS.gene066535:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG - chr7.1:2488117-2488136 MS.gene066535:intron 55.0%
CCTCAGCAGCGAGATACTCA+AGG + chr7.1:2487990-2488009 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG - chr7.1:2487987-2488006 MS.gene066535:CDS 55.0%
TCCACTGGCACAACTAAGGG+CGG - chr7.1:2487817-2487836 MS.gene066535:CDS 55.0%
TGGCACAACTAAGGGCGGAA+GGG - chr7.1:2487822-2487841 MS.gene066535:CDS 55.0%
CGGCGTATTTACCGGCCTTG+AGG + chr7.1:2487927-2487946 None:intergenic 60.0%
CTGGCACAACTAAGGGCGGA+AGG - chr7.1:2487821-2487840 MS.gene066535:CDS 60.0%
GATACTCAAGGACGGCGGAG+AGG + chr7.1:2487978-2487997 None:intergenic 60.0%
GGCACAACTAAGGGCGGAAG+GGG - chr7.1:2487823-2487842 MS.gene066535:CDS 60.0%
TCCGCCCTTAGTTGTGCCAG+TGG + chr7.1:2487821-2487840 None:intergenic 60.0%
! GGAGAGGTAGACAGGAGCAC+CGG + chr7.1:2487962-2487981 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG - chr7.1:2487940-2487959 MS.gene066535:CDS 60.0%
!! AGCACCGGCACCAACACGTT+CGG + chr7.1:2487947-2487966 None:intergenic 60.0%
AGGACGGCGGAGAGGTAGAC+AGG + chr7.1:2487970-2487989 None:intergenic 65.0%
GGGCGGAAGGGGTAAGCCAA+AGG - chr7.1:2487834-2487853 MS.gene066535:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 2487811 2488330 2487811 ID=MS.gene066535
chr7.1 mRNA 2487811 2488330 2487811 ID=MS.gene066535.t1;Parent=MS.gene066535
chr7.1 exon 2488133 2488330 2488133 ID=MS.gene066535.t1.exon1;Parent=MS.gene066535.t1
chr7.1 CDS 2488133 2488330 2488133 ID=cds.MS.gene066535.t1;Parent=MS.gene066535.t1
chr7.1 exon 2487811 2488032 2487811 ID=MS.gene066535.t1.exon2;Parent=MS.gene066535.t1
chr7.1 CDS 2487811 2488032 2487811 ID=cds.MS.gene066535.t1;Parent=MS.gene066535.t1
Gene Sequence

>MS.gene066535

ATGAGTTCCACTGGCACAACTAAGGGCGGAAGGGGTAAGCCAAAGGCTGCCAAATCAGTTTCAAGATCTTCAAAGGCAGGTCTTCAATTTCCCGTCGGAAGAATCGCGAGATTCCTCAAGGCCGGTAAATACGCCGAACGTGTTGGTGCCGGTGCTCCTGTCTACCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTTTGTTTCCTTTCTTCAATTTCCCTTTCACTCTCTTTTTTGTATAATGTAGGGTTCTAGATCCAATTTGATTAAACTGTATTTTCATTTTGGTATTTAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTTAGGAACGATGAAGAACTCAGCAAGCTTTTGGGGACTGTGACCATTGCTAATGGTGGTGTTTTACCTAATATTCATCAGACTTTGCTACCTAAGAAGGTTGGAAAGGGCAAAGGAGAAATTGGATCTGCTTCTCAAGAATTCTAG

Protein sequence

>MS.gene066535.t1

MSSTGTTKGGRGKPKAAKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF