AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78324


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78324.t1 MTR_7g114060 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene78324.t1 MTR_7g114040 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene78324.t1 MTR_7g108320 95.775 142 3 1 1 139 1 142 4.40e-93 265
MS.gene78324.t1 MTR_7g093910 87.313 134 17 0 6 139 9 142 6.22e-82 237
MS.gene78324.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 3.86e-72 212
MS.gene78324.t1 MTR_2g082510 86.777 121 12 1 10 126 3 123 3.42e-69 204
MS.gene78324.t1 MTR_2g082220 84.921 126 11 2 10 129 3 126 4.30e-69 204
MS.gene78324.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 1.15e-59 181
MS.gene78324.t1 MTR_7g113160 76.068 117 28 0 9 125 16 132 3.78e-59 179
MS.gene78324.t1 MTR_4g063280 79.091 110 23 0 18 127 17 126 2.93e-58 177
MS.gene78324.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 5.36e-58 177
MS.gene78324.t1 MTR_4g063410 79.439 107 22 0 19 125 24 130 5.44e-58 177
MS.gene78324.t1 MTR_4g071150 80.374 107 21 0 19 125 24 130 6.46e-58 177
MS.gene78324.t1 MTR_4g064005 81.132 106 20 0 19 124 24 129 6.49e-58 177
MS.gene78324.t1 MTR_8g086640 81.132 106 20 0 19 124 24 129 7.30e-58 177
MS.gene78324.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 8.16e-58 176
MS.gene78324.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 5.87e-42 136
MS.gene78324.t1 MTR_1g011750 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene78324.t1 MTR_1g011850 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene78324.t1 MTR_1g114050 62.857 105 37 2 20 123 24 127 5.35e-38 125
MS.gene78324.t1 MTR_1g071040 63.462 104 36 2 21 123 28 130 1.15e-37 125
MS.gene78324.t1 MTR_4g063300 67.708 96 23 1 40 127 10 105 7.05e-36 120
MS.gene78324.t1 MTR_4g005910 88.889 45 5 0 82 126 4 48 6.44e-23 85.5
MS.gene78324.t1 MTR_7g010440 54.444 90 41 0 47 136 96 185 2.92e-22 89.7
MS.gene78324.t1 MTR_4g048490 44.860 107 30 2 19 125 26 103 2.83e-20 80.1
MS.gene78324.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 1.19e-18 75.9
MS.gene78324.t1 MTR_7g027610 41.818 110 32 3 18 123 13 94 8.02e-16 68.9
MS.gene78324.t1 MTR_0653s0030 52.830 53 25 0 50 102 43 95 4.63e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78324.t1 AT1G54690 92.958 142 7 1 1 139 1 142 2.22e-90 258
MS.gene78324.t1 AT1G08880 94.118 136 8 0 4 139 7 142 5.41e-90 258
MS.gene78324.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene78324.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene78324.t1 AT5G54640 82.946 129 19 1 1 129 1 126 1.59e-69 205
MS.gene78324.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.51e-68 203
MS.gene78324.t1 AT3G20670 84.677 124 15 1 10 129 3 126 4.76e-68 202
MS.gene78324.t1 AT5G59870 73.643 129 29 2 1 124 1 129 1.24e-60 184
MS.gene78324.t1 AT5G27670 73.438 128 29 1 2 124 3 130 4.78e-58 177
MS.gene78324.t1 AT5G02560 79.439 107 22 0 18 124 23 129 5.23e-57 174
MS.gene78324.t1 AT5G02560 64.885 131 22 1 18 124 23 153 1.37e-51 162
MS.gene78324.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78324.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78324.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78324.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78324.t1 AT1G52740 61.905 105 38 2 20 123 27 130 2.42e-38 127
MS.gene78324.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene78324.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene78324.t1 AT4G13570 43.220 118 65 2 1 117 2 118 3.98e-23 87.8
MS.gene78324.t1 AT4G13570 49.451 91 44 2 28 117 35 124 9.39e-22 84.3

Find 49 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCCTTTCCAACCTTCTT+AGG 0.222545 7.4:-2864385 None:intergenic
ATCTGCTTCTCAAGAATTCT+AGG 0.264115 7.4:+2864420 MS.gene78324:CDS
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.4:+2864306 MS.gene78324:CDS
GAAACTGATTTGGCAGCCTT+TGG 0.309618 7.4:-2863961 None:intergenic
TGAAGATCTTGAAACTGATT+TGG 0.311823 7.4:-2863971 None:intergenic
AACACGTTCGGCGTATTTAC+CGG 0.321195 7.4:-2864043 None:intergenic
GGAAAGGGCAAAGGAGAAAT+TGG 0.326882 7.4:+2864397 MS.gene78324:CDS
AGCAAAGTCTGATGAATATT+AGG 0.347792 7.4:-2864361 None:intergenic
TCAGACTTTGCTACCTAAGA+AGG 0.421667 7.4:+2864372 MS.gene78324:CDS
GGCACATTCAGCTTGCTGTT+AGG 0.440630 7.4:+2864278 MS.gene78324:CDS
GAGTTCCACTGGCACAACTA+AGG 0.456467 7.4:+2863924 MS.gene78324:CDS
AGGACGGCGGAGAGGTAGAC+AGG 0.463287 7.4:-2864078 None:intergenic
GGGACTGTGACCATTGCTAA+TGG 0.468303 7.4:+2864328 MS.gene78324:CDS
AGCACCGGCACCAACACGTT+CGG 0.480590 7.4:-2864055 None:intergenic
GTTTCAAGATCTTCAAAGGC+AGG 0.496108 7.4:+2863979 MS.gene78324:CDS
CTGGCACAACTAAGGGCGGA+AGG 0.502885 7.4:+2863932 MS.gene78324:CDS
ATCAGTTTCAAGATCTTCAA+AGG 0.503488 7.4:+2863975 MS.gene78324:CDS
CTACCTAAGAAGGTTGGAAA+GGG 0.510822 7.4:+2864382 MS.gene78324:CDS
TCTGCTTCTCAAGAATTCTA+GGG 0.519604 7.4:+2864421 MS.gene78324:CDS
GGCACAACTAAGGGCGGAAG+GGG 0.525782 7.4:+2863934 MS.gene78324:CDS
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7.4:+2864227 MS.gene78324:CDS
ACTTTGCTACCTAAGAAGGT+TGG 0.535784 7.4:+2864376 MS.gene78324:CDS
TGGCACAACTAAGGGCGGAA+GGG 0.543224 7.4:+2863933 MS.gene78324:CDS
AGTTCCACTGGCACAACTAA+GGG 0.549244 7.4:+2863925 MS.gene78324:CDS
TACGCCGAACGTGTTGGTGC+CGG 0.556200 7.4:+2864051 MS.gene78324:CDS
GCTACCTAAGAAGGTTGGAA+AGG 0.557785 7.4:+2864381 MS.gene78324:CDS
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7.4:+2864228 MS.gene78324:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.570928 7.4:-2864274 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7.4:-2864264 None:intergenic
GGGCGGAAGGGGTAAGCCAA+AGG 0.573881 7.4:+2863945 MS.gene78324:CDS
CGGCGTATTTACCGGCCTTG+AGG 0.577173 7.4:-2864035 None:intergenic
ATCGCGAGATTCCTCAAGGC+CGG 0.578871 7.4:+2864024 MS.gene78324:CDS
TCCGCCCTTAGTTGTGCCAG+TGG 0.580065 7.4:-2863929 None:intergenic
GAATCTCGCGATTCTTCCGA+CGG 0.592664 7.4:-2864013 None:intergenic
GGTAAATACGCCGAACGTGT+TGG 0.593895 7.4:+2864045 MS.gene78324:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.612329 7.4:+2864098 MS.gene78324:CDS
AAGAAGGTTGGAAAGGGCAA+AGG 0.621113 7.4:+2864388 MS.gene78324:CDS
AATCTCGCGATTCTTCCGAC+GGG 0.626786 7.4:-2864012 None:intergenic
TAAAACACCACCATTAGCAA+TGG 0.630364 7.4:-2864338 None:intergenic
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.4:-2864098 None:intergenic
GGAGAGGTAGACAGGAGCAC+CGG 0.644805 7.4:-2864070 None:intergenic
TCCACTGGCACAACTAAGGG+CGG 0.647888 7.4:+2863928 MS.gene78324:CDS
AAGAATCGCGAGATTCCTCA+AGG 0.651972 7.4:+2864020 MS.gene78324:CDS
ACTGTGACCATTGCTAATGG+TGG 0.653058 7.4:+2864331 MS.gene78324:CDS
GCAGGTCTTCAATTTCCCGT+CGG 0.653866 7.4:+2863997 MS.gene78324:CDS
GATACTCAAGGACGGCGGAG+AGG 0.669935 7.4:-2864086 None:intergenic
AGCAGCGAGATACTCAAGGA+CGG 0.684917 7.4:-2864094 None:intergenic
AGAAGAACCGTATTGTACCA+AGG 0.686303 7.4:+2864257 MS.gene78324:CDS
AGCGAGATACTCAAGGACGG+CGG 0.736224 7.4:-2864091 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAGTTTAATCAAAT+TGG - chr7.4:2864185-2864204 None:intergenic 15.0%
!!! ATTAAACTGTATTTTCATTT+TGG + chr7.4:2864190-2864209 MS.gene78324:intron 15.0%
!!! GTATTTTCATTTTGGTATTT+AGG + chr7.4:2864198-2864217 MS.gene78324:intron 20.0%
!!! TCTCTTTTTTGTATAATGTA+GGG + chr7.4:2864151-2864170 MS.gene78324:intron 20.0%
! ATACAAAAAAGAGAGTGAAA+GGG - chr7.4:2864145-2864164 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG - chr7.4:2864146-2864165 None:intergenic 25.0%
!!! CTCTCTTTTTTGTATAATGT+AGG + chr7.4:2864150-2864169 MS.gene78324:intron 25.0%
!!! TCATTTTGGTATTTAGGTTT+TGG + chr7.4:2864204-2864223 MS.gene78324:intron 25.0%
AGCAAAGTCTGATGAATATT+AGG - chr7.4:2864364-2864383 None:intergenic 30.0%
ATCAGTTTCAAGATCTTCAA+AGG + chr7.4:2863975-2863994 MS.gene78324:CDS 30.0%
TGAAGATCTTGAAACTGATT+TGG - chr7.4:2863974-2863993 None:intergenic 30.0%
!!! TGGTATTTAGGTTTTGGAAT+TGG + chr7.4:2864210-2864229 MS.gene78324:intron 30.0%
GTGAAAGGGAAATTGAAGAA+AGG - chr7.4:2864131-2864150 None:intergenic 35.0%
TAAAACACCACCATTAGCAA+TGG - chr7.4:2864341-2864360 None:intergenic 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG + chr7.4:2864214-2864233 MS.gene78324:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG + chr7.4:2864215-2864234 MS.gene78324:intron 35.0%
ACTTTGCTACCTAAGAAGGT+TGG + chr7.4:2864376-2864395 MS.gene78324:CDS 40.0%
AGAAGAACCGTATTGTACCA+AGG + chr7.4:2864257-2864276 MS.gene78324:CDS 40.0%
CTACCTAAGAAGGTTGGAAA+GGG + chr7.4:2864382-2864401 MS.gene78324:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG - chr7.4:2864267-2864286 None:intergenic 40.0%
GTTTCAAGATCTTCAAAGGC+AGG + chr7.4:2863979-2863998 MS.gene78324:CDS 40.0%
TCAGACTTTGCTACCTAAGA+AGG + chr7.4:2864372-2864391 MS.gene78324:CDS 40.0%
! AAGAACTCAGCAAGCTTTTG+GGG + chr7.4:2864308-2864327 MS.gene78324:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7.4:2864307-2864326 MS.gene78324:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7.4:2864306-2864325 MS.gene78324:CDS 40.0%
AACACGTTCGGCGTATTTAC+CGG - chr7.4:2864046-2864065 None:intergenic 45.0%
AAGAAGGTTGGAAAGGGCAA+AGG + chr7.4:2864388-2864407 MS.gene78324:CDS 45.0%
AAGAATCGCGAGATTCCTCA+AGG + chr7.4:2864020-2864039 MS.gene78324:CDS 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7.4:2864331-2864350 MS.gene78324:CDS 45.0%
AGTTCCACTGGCACAACTAA+GGG + chr7.4:2863925-2863944 MS.gene78324:CDS 45.0%
GCTACCTAAGAAGGTTGGAA+AGG + chr7.4:2864381-2864400 MS.gene78324:CDS 45.0%
GGAAAGGGCAAAGGAGAAAT+TGG + chr7.4:2864397-2864416 MS.gene78324:CDS 45.0%
TTGCCCTTTCCAACCTTCTT+AGG - chr7.4:2864388-2864407 None:intergenic 45.0%
!! GAAACTGATTTGGCAGCCTT+TGG - chr7.4:2863964-2863983 None:intergenic 45.0%
AATCTCGCGATTCTTCCGAC+GGG - chr7.4:2864015-2864034 None:intergenic 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7.4:2864277-2864296 None:intergenic 50.0%
AGCAGCGAGATACTCAAGGA+CGG - chr7.4:2864097-2864116 None:intergenic 50.0%
GAATCTCGCGATTCTTCCGA+CGG - chr7.4:2864016-2864035 None:intergenic 50.0%
GAGTTCCACTGGCACAACTA+AGG + chr7.4:2863924-2863943 MS.gene78324:CDS 50.0%
GCAGGTCTTCAATTTCCCGT+CGG + chr7.4:2863997-2864016 MS.gene78324:CDS 50.0%
GGGACTGTGACCATTGCTAA+TGG + chr7.4:2864328-2864347 MS.gene78324:CDS 50.0%
GGTAAATACGCCGAACGTGT+TGG + chr7.4:2864045-2864064 MS.gene78324:CDS 50.0%
!! GGCACATTCAGCTTGCTGTT+AGG + chr7.4:2864278-2864297 MS.gene78324:CDS 50.0%
AATTGGCTGGGAACGCTGCA+AGG + chr7.4:2864227-2864246 MS.gene78324:CDS 55.0%
AGCGAGATACTCAAGGACGG+CGG - chr7.4:2864094-2864113 None:intergenic 55.0%
ATCGCGAGATTCCTCAAGGC+CGG + chr7.4:2864024-2864043 MS.gene78324:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG + chr7.4:2864228-2864247 MS.gene78324:CDS 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7.4:2864101-2864120 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7.4:2864098-2864117 MS.gene78324:CDS 55.0%
TCCACTGGCACAACTAAGGG+CGG + chr7.4:2863928-2863947 MS.gene78324:CDS 55.0%
TGGCACAACTAAGGGCGGAA+GGG + chr7.4:2863933-2863952 MS.gene78324:CDS 55.0%
CGGCGTATTTACCGGCCTTG+AGG - chr7.4:2864038-2864057 None:intergenic 60.0%
CTGGCACAACTAAGGGCGGA+AGG + chr7.4:2863932-2863951 MS.gene78324:CDS 60.0%
GATACTCAAGGACGGCGGAG+AGG - chr7.4:2864089-2864108 None:intergenic 60.0%
GGCACAACTAAGGGCGGAAG+GGG + chr7.4:2863934-2863953 MS.gene78324:CDS 60.0%
TCCGCCCTTAGTTGTGCCAG+TGG - chr7.4:2863932-2863951 None:intergenic 60.0%
! GGAGAGGTAGACAGGAGCAC+CGG - chr7.4:2864073-2864092 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7.4:2864051-2864070 MS.gene78324:CDS 60.0%
!! AGCACCGGCACCAACACGTT+CGG - chr7.4:2864058-2864077 None:intergenic 60.0%
AGGACGGCGGAGAGGTAGAC+AGG - chr7.4:2864081-2864100 None:intergenic 65.0%
GGGCGGAAGGGGTAAGCCAA+AGG + chr7.4:2863945-2863964 MS.gene78324:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 2863922 2864441 2863922 ID=MS.gene78324
chr7.4 mRNA 2863922 2864441 2863922 ID=MS.gene78324.t1;Parent=MS.gene78324
chr7.4 exon 2863922 2864119 2863922 ID=MS.gene78324.t1.exon1;Parent=MS.gene78324.t1
chr7.4 CDS 2863922 2864119 2863922 ID=cds.MS.gene78324.t1;Parent=MS.gene78324.t1
chr7.4 exon 2864220 2864441 2864220 ID=MS.gene78324.t1.exon2;Parent=MS.gene78324.t1
chr7.4 CDS 2864220 2864441 2864220 ID=cds.MS.gene78324.t1;Parent=MS.gene78324.t1
Gene Sequence

>MS.gene78324

ATGAGTTCCACTGGCACAACTAAGGGCGGAAGGGGTAAGCCAAAGGCTGCCAAATCAGTTTCAAGATCTTCAAAGGCAGGTCTTCAATTTCCCGTCGGAAGAATCGCGAGATTCCTCAAGGCCGGTAAATACGCCGAACGTGTTGGTGCCGGTGCTCCTGTCTACCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTTTGTTTCCTTTCTTCAATTTCCCTTTCACTCTCTTTTTTGTATAATGTAGGGTTCTAGATCCAATTTGATTAAACTGTATTTTCATTTTGGTATTTAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTTAGGAACGATGAAGAACTCAGCAAGCTTTTGGGGACTGTGACCATTGCTAATGGTGGTGTTTTACCTAATATTCATCAGACTTTGCTACCTAAGAAGGTTGGAAAGGGCAAAGGAGAAATTGGATCTGCTTCTCAAGAATTCTAG

Protein sequence

>MS.gene78324.t1

MSSTGTTKGGRGKPKAAKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF