AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78323


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78323.t1 MTR_7g114060 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene78323.t1 MTR_7g114040 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene78323.t1 MTR_7g108320 95.070 142 4 1 1 139 1 142 3.24e-92 263
MS.gene78323.t1 MTR_7g093910 85.401 137 20 0 3 139 6 142 4.01e-82 238
MS.gene78323.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 9.58e-73 213
MS.gene78323.t1 MTR_2g082510 87.603 121 11 1 10 126 3 123 6.39e-70 206
MS.gene78323.t1 MTR_2g082220 86.290 124 13 1 10 129 3 126 7.87e-70 206
MS.gene78323.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 7.95e-60 181
MS.gene78323.t1 MTR_7g113160 76.033 121 25 1 4 124 15 131 3.14e-59 180
MS.gene78323.t1 MTR_4g063280 72.441 127 34 1 1 127 1 126 2.43e-58 177
MS.gene78323.t1 MTR_8g086640 79.817 109 22 0 19 127 24 132 2.79e-58 177
MS.gene78323.t1 MTR_4g064005 79.817 109 22 0 19 127 24 132 4.57e-58 177
MS.gene78323.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 4.86e-58 177
MS.gene78323.t1 MTR_4g071150 81.132 106 20 0 19 124 24 129 6.90e-58 177
MS.gene78323.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 9.83e-58 176
MS.gene78323.t1 MTR_4g063410 80.189 106 21 0 19 124 24 129 1.90e-57 176
MS.gene78323.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 3.71e-42 136
MS.gene78323.t1 MTR_1g011750 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene78323.t1 MTR_1g011850 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene78323.t1 MTR_1g114050 62.617 107 38 2 20 125 24 129 1.90e-38 127
MS.gene78323.t1 MTR_1g071040 63.208 106 37 2 21 125 28 132 3.90e-38 126
MS.gene78323.t1 MTR_4g063300 79.730 74 15 0 40 113 10 83 9.89e-36 119
MS.gene78323.t1 MTR_4g005910 91.111 45 4 0 82 126 4 48 1.24e-23 87.0
MS.gene78323.t1 MTR_7g010440 69.697 66 20 0 73 138 122 187 5.68e-23 91.7
MS.gene78323.t1 MTR_4g048490 45.714 105 28 2 19 123 26 101 6.65e-20 79.0
MS.gene78323.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 6.85e-19 76.6
MS.gene78323.t1 MTR_7g027610 40.323 124 39 4 6 125 4 96 2.25e-16 70.1
MS.gene78323.t1 MTR_0653s0030 40.000 100 35 2 3 102 21 95 1.95e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78323.t1 AT1G54690 94.118 136 8 0 4 139 7 142 9.16e-90 257
MS.gene78323.t1 AT1G08880 93.382 136 9 0 4 139 7 142 2.28e-89 256
MS.gene78323.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene78323.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene78323.t1 AT5G54640 86.290 124 13 1 10 129 3 126 9.30e-70 206
MS.gene78323.t1 AT3G20670 85.484 124 14 1 10 129 3 126 1.04e-68 203
MS.gene78323.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.49e-68 203
MS.gene78323.t1 AT5G59870 72.308 130 31 2 1 125 1 130 1.56e-60 183
MS.gene78323.t1 AT5G27670 70.588 136 34 2 4 137 14 145 1.16e-57 176
MS.gene78323.t1 AT5G02560 78.182 110 24 0 18 127 23 132 1.81e-57 176
MS.gene78323.t1 AT5G02560 64.179 134 24 1 18 127 23 156 4.48e-52 163
MS.gene78323.t1 AT1G52740 57.377 122 43 3 12 125 12 132 6.98e-39 128
MS.gene78323.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene78323.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene78323.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene78323.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene78323.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene78323.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene78323.t1 AT4G13570 45.045 111 59 2 8 117 9 118 2.09e-22 85.9
MS.gene78323.t1 AT4G13570 49.451 91 44 2 28 117 35 124 8.80e-22 84.3

Find 51 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAAAGTTTGATGAATAT+TGG 0.233140 7.4:+2867803 None:intergenic
TTTCCCTTCCCAGCCTTCTT+TGG 0.272919 7.4:+2867780 None:intergenic
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.4:-2867859 MS.gene78323:CDS
TGAAGATCTGGAAACTGATT+TGG 0.302821 7.4:+2868195 None:intergenic
GGGAAGGGAAAAGGCGAAAT+TGG 0.311073 7.4:-2867768 MS.gene78323:CDS
AACACGTTCGGCGTATTTGC+CGG 0.318153 7.4:+2868123 None:intergenic
AGCAAAGTTTGATGAATATT+GGG 0.340383 7.4:+2867804 None:intergenic
AAGTCCGGCCTTTGAAGATC+TGG 0.387108 7.4:+2868183 None:intergenic
GAAACTGATTTGGAAGCCTT+CGG 0.412102 7.4:+2868205 None:intergenic
TCAAACTTTGCTGCCAAAGA+AGG 0.417745 7.4:-2867793 MS.gene78323:CDS
ACTTTGCTGCCAAAGAAGGC+TGG 0.417932 7.4:-2867789 MS.gene78323:CDS
AGCTCCGGCACCAACACGTT+CGG 0.444935 7.4:+2868111 None:intergenic
AAGAAGGCTGGGAAGGGAAA+AGG 0.471306 7.4:-2867777 MS.gene78323:CDS
GGAACTGTGACCATTGCTAA+TGG 0.483635 7.4:-2867837 MS.gene78323:CDS
ACGTGTTGGTGCCGGAGCTC+CGG 0.485122 7.4:-2868107 MS.gene78323:CDS
GGCACATTCAGCTTGCTGTC+AGG 0.491110 7.4:-2867887 MS.gene78323:CDS
CGGCGTATTTGCCGGCCTTG+AGG 0.502918 7.4:+2868131 None:intergenic
CTGGTACCACCAAGGGAGGA+AGG 0.503740 7.4:-2868234 MS.gene78323:CDS
GGTACCACCAAGGGAGGAAG+GGG 0.506354 7.4:-2868232 MS.gene78323:CDS
CTTTGCTGCCAAAGAAGGCT+GGG 0.509397 7.4:-2867788 MS.gene78323:CDS
GTTTCCAGATCTTCAAAGGC+CGG 0.518477 7.4:-2868187 MS.gene78323:CDS
TCCGACGGGGAATTGAAGTC+CGG 0.524129 7.4:+2868168 None:intergenic
TACGCCGAACGTGTTGGTGC+CGG 0.525825 7.4:-2868115 MS.gene78323:CDS
GCTGCCAAAGAAGGCTGGGA+AGG 0.531759 7.4:-2867784 MS.gene78323:CDS
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7.4:-2867938 MS.gene78323:CDS
ATCGCAAGATTCCTCAAGGC+CGG 0.538535 7.4:-2868142 MS.gene78323:CDS
TGGTACCACCAAGGGAGGAA+GGG 0.539265 7.4:-2868233 MS.gene78323:CDS
ATCAGTTTCCAGATCTTCAA+AGG 0.543380 7.4:-2868191 MS.gene78323:CDS
AGAGAGGTATACCGGAGCTC+CGG 0.548996 7.4:+2868096 None:intergenic
AGGACCGCAGAGAGGTATAC+CGG 0.559095 7.4:+2868088 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.559881 7.4:+2868154 None:intergenic
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7.4:-2867937 MS.gene78323:CDS
GATTCTGCTGGTACCACCAA+GGG 0.568843 7.4:-2868241 MS.gene78323:CDS
GAATCTTGCGATTCTTCCGA+CGG 0.569168 7.4:+2868153 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7.4:+2867901 None:intergenic
CTGCCAAAGAAGGCTGGGAA+GGG 0.583937 7.4:-2867783 MS.gene78323:CDS
GGCAAATACGCCGAACGTGT+TGG 0.584120 7.4:-2868121 MS.gene78323:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.605047 7.4:+2867891 None:intergenic
GGGAGGAAGGGGAAAACCGA+AGG 0.611626 7.4:-2868221 MS.gene78323:CDS
AAGAATCGCAAGATTCCTCA+AGG 0.619754 7.4:-2868146 MS.gene78323:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.632170 7.4:-2868068 MS.gene78323:intron
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.4:+2868068 None:intergenic
TCTGCTGGTACCACCAAGGG+AGG 0.639099 7.4:-2868238 MS.gene78323:CDS
CAGAACACCACCATTAGCAA+TGG 0.646082 7.4:+2867827 None:intergenic
GCCGGACTTCAATTCCCCGT+CGG 0.658042 7.4:-2868169 MS.gene78323:CDS
GGATTCTGCTGGTACCACCA+AGG 0.659312 7.4:-2868242 MS.gene78323:CDS
AGAAGAACCGTATTGTACCA+AGG 0.686303 7.4:-2867908 MS.gene78323:CDS
ACTGTGACCATTGCTAATGG+TGG 0.694716 7.4:-2867834 MS.gene78323:CDS
ATCTTGCGATTCTTCCGACG+GGG 0.695192 7.4:+2868155 None:intergenic
GATACTCAAGGACCGCAGAG+AGG 0.712819 7.4:+2868080 None:intergenic
AGCTCCGGTATACCTCTCTG+CGG 0.763589 7.4:-2868092 MS.gene78323:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAACTGTATTTTAATTT+TGG - chr7.4:2868014-2868033 MS.gene78323:intron 10.0%
!!! TTAAACTGTATTTTAATTTT+GGG - chr7.4:2868015-2868034 MS.gene78323:intron 10.0%
!! AAAATACAGTTTAATCAAAT+TGG + chr7.4:2868009-2868028 None:intergenic 15.0%
!!! TATTTTAATTTTGGGTATTT+AGG - chr7.4:2868023-2868042 MS.gene78323:intron 15.0%
!!! TCTCTTTTTTGTATAATTTA+GGG - chr7.4:2867975-2867994 MS.gene78323:intron 15.0%
!!! CTCTCTTTTTTGTATAATTT+AGG - chr7.4:2867974-2867993 MS.gene78323:intron 20.0%
! AGCAAAGTTTGATGAATATT+GGG + chr7.4:2868189-2868208 None:intergenic 25.0%
! ATACAAAAAAGAGAGTGAAA+GGG + chr7.4:2867969-2867988 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG + chr7.4:2867970-2867989 None:intergenic 25.0%
!!! AATTTTGGGTATTTAGGTTT+TGG - chr7.4:2868029-2868048 MS.gene78323:intron 25.0%
CAGCAAAGTTTGATGAATAT+TGG + chr7.4:2868190-2868209 None:intergenic 30.0%
ATCAGTTTCCAGATCTTCAA+AGG - chr7.4:2867799-2867818 MS.gene78323:CDS 35.0%
TGAAGATCTGGAAACTGATT+TGG + chr7.4:2867798-2867817 None:intergenic 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG - chr7.4:2868039-2868058 MS.gene78323:intron 35.0%
!!! GGGTATTTAGGTTTTGGAAT+TGG - chr7.4:2868035-2868054 MS.gene78323:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG - chr7.4:2868040-2868059 MS.gene78323:intron 35.0%
AAGAATCGCAAGATTCCTCA+AGG - chr7.4:2867844-2867863 MS.gene78323:CDS 40.0%
AGAAGAACCGTATTGTACCA+AGG - chr7.4:2868082-2868101 MS.gene78323:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG + chr7.4:2868092-2868111 None:intergenic 40.0%
TCAAACTTTGCTGCCAAAGA+AGG - chr7.4:2868197-2868216 MS.gene78323:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG - chr7.4:2868132-2868151 MS.gene78323:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG - chr7.4:2868131-2868150 MS.gene78323:CDS 40.0%
!! GAAACTGATTTGGAAGCCTT+CGG + chr7.4:2867788-2867807 None:intergenic 40.0%
AATCTTGCGATTCTTCCGAC+GGG + chr7.4:2867839-2867858 None:intergenic 45.0%
ACTGTGACCATTGCTAATGG+TGG - chr7.4:2868156-2868175 MS.gene78323:CDS 45.0%
CAGAACACCACCATTAGCAA+TGG + chr7.4:2868166-2868185 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG + chr7.4:2867840-2867859 None:intergenic 45.0%
GGAACTGTGACCATTGCTAA+TGG - chr7.4:2868153-2868172 MS.gene78323:CDS 45.0%
GTTTCCAGATCTTCAAAGGC+CGG - chr7.4:2867803-2867822 MS.gene78323:CDS 45.0%
AACACGTTCGGCGTATTTGC+CGG + chr7.4:2867870-2867889 None:intergenic 50.0%
AAGAAGGCTGGGAAGGGAAA+AGG - chr7.4:2868213-2868232 MS.gene78323:CDS 50.0%
ACAGCAAGCTGAATGTGCCT+TGG + chr7.4:2868102-2868121 None:intergenic 50.0%
ACTTTGCTGCCAAAGAAGGC+TGG - chr7.4:2868201-2868220 MS.gene78323:CDS 50.0%
ATCGCAAGATTCCTCAAGGC+CGG - chr7.4:2867848-2867867 MS.gene78323:CDS 50.0%
ATCTTGCGATTCTTCCGACG+GGG + chr7.4:2867838-2867857 None:intergenic 50.0%
CTTTGCTGCCAAAGAAGGCT+GGG - chr7.4:2868202-2868221 MS.gene78323:CDS 50.0%
GATTCTGCTGGTACCACCAA+GGG - chr7.4:2867749-2867768 MS.gene78323:CDS 50.0%
GGGAAGGGAAAAGGCGAAAT+TGG - chr7.4:2868222-2868241 MS.gene78323:CDS 50.0%
TTTCCCTTCCCAGCCTTCTT+TGG + chr7.4:2868213-2868232 None:intergenic 50.0%
! AAGTCCGGCCTTTGAAGATC+TGG + chr7.4:2867810-2867829 None:intergenic 50.0%
AATTGGCTGGGAACGCTGCA+AGG - chr7.4:2868052-2868071 MS.gene78323:intron 55.0%
AGCTCCGGTATACCTCTCTG+CGG - chr7.4:2867898-2867917 MS.gene78323:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG - chr7.4:2868053-2868072 MS.gene78323:intron 55.0%
CCTCAGCAGCGAGATACTCA+AGG + chr7.4:2867925-2867944 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG - chr7.4:2867922-2867941 MS.gene78323:CDS 55.0%
CTGCCAAAGAAGGCTGGGAA+GGG - chr7.4:2868207-2868226 MS.gene78323:CDS 55.0%
GATACTCAAGGACCGCAGAG+AGG + chr7.4:2867913-2867932 None:intergenic 55.0%
GGATTCTGCTGGTACCACCA+AGG - chr7.4:2867748-2867767 MS.gene78323:CDS 55.0%
GGCAAATACGCCGAACGTGT+TGG - chr7.4:2867869-2867888 MS.gene78323:CDS 55.0%
TCCGACGGGGAATTGAAGTC+CGG + chr7.4:2867825-2867844 None:intergenic 55.0%
TGGTACCACCAAGGGAGGAA+GGG - chr7.4:2867757-2867776 MS.gene78323:CDS 55.0%
! AGAGAGGTATACCGGAGCTC+CGG + chr7.4:2867897-2867916 None:intergenic 55.0%
! AGGACCGCAGAGAGGTATAC+CGG + chr7.4:2867905-2867924 None:intergenic 55.0%
! TTTTCCCCTTCCTCCCTTGG+TGG + chr7.4:2867765-2867784 None:intergenic 55.0%
!! GGCACATTCAGCTTGCTGTC+AGG - chr7.4:2868103-2868122 MS.gene78323:CDS 55.0%
AGCTCCGGCACCAACACGTT+CGG + chr7.4:2867882-2867901 None:intergenic 60.0%
CTGGTACCACCAAGGGAGGA+AGG - chr7.4:2867756-2867775 MS.gene78323:CDS 60.0%
GCCGGACTTCAATTCCCCGT+CGG - chr7.4:2867821-2867840 MS.gene78323:CDS 60.0%
GCTGCCAAAGAAGGCTGGGA+AGG - chr7.4:2868206-2868225 MS.gene78323:CDS 60.0%
GGGAGGAAGGGGAAAACCGA+AGG - chr7.4:2867769-2867788 MS.gene78323:CDS 60.0%
GGTACCACCAAGGGAGGAAG+GGG - chr7.4:2867758-2867777 MS.gene78323:CDS 60.0%
TCTGCTGGTACCACCAAGGG+AGG - chr7.4:2867752-2867771 MS.gene78323:CDS 60.0%
! CGGTTTTCCCCTTCCTCCCT+TGG + chr7.4:2867768-2867787 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG - chr7.4:2867875-2867894 MS.gene78323:CDS 60.0%
CGGCGTATTTGCCGGCCTTG+AGG + chr7.4:2867862-2867881 None:intergenic 65.0%
!! ACGTGTTGGTGCCGGAGCTC+CGG - chr7.4:2867883-2867902 MS.gene78323:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 2867746 2868266 2867746 ID=MS.gene78323
chr7.4 mRNA 2867746 2868266 2867746 ID=MS.gene78323.t1;Parent=MS.gene78323
chr7.4 exon 2868069 2868266 2868069 ID=MS.gene78323.t1.exon1;Parent=MS.gene78323.t1
chr7.4 CDS 2868069 2868266 2868069 ID=cds.MS.gene78323.t1;Parent=MS.gene78323.t1
chr7.4 exon 2867746 2867967 2867746 ID=MS.gene78323.t1.exon2;Parent=MS.gene78323.t1
chr7.4 CDS 2867746 2867967 2867746 ID=cds.MS.gene78323.t1;Parent=MS.gene78323.t1
Gene Sequence

>MS.gene78323

ATGGATTCTGCTGGTACCACCAAGGGAGGAAGGGGAAAACCGAAGGCTTCCAAATCAGTTTCCAGATCTTCAAAGGCCGGACTTCAATTCCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCCGGCAAATACGCCGAACGTGTTGGTGCCGGAGCTCCGGTATACCTCTCTGCGGTCCTTGAGTATCTCGCTGCTGAGGTTCGTTTCTTTTCTTCAATTTCCCTTTCACTCTCTTTTTTGTATAATTTAGGGTTCTAGATCCAATTTGATTAAACTGTATTTTAATTTTGGGTATTTAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTCAGGAACGATGAAGAACTCAGCAAGCTTTTGGGAACTGTGACCATTGCTAATGGTGGTGTTCTGCCCAATATTCATCAAACTTTGCTGCCAAAGAAGGCTGGGAAGGGAAAAGGCGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>MS.gene78323.t1

MDSAGTTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKAGKGKGEIGSASQEF