AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78322


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78322.t1 MTR_7g114060 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene78322.t1 MTR_7g114040 100.000 139 0 0 1 139 1 139 3.00e-97 276
MS.gene78322.t1 MTR_7g108320 95.775 142 3 1 1 139 1 142 4.40e-93 265
MS.gene78322.t1 MTR_7g093910 87.313 134 17 0 6 139 9 142 6.22e-82 237
MS.gene78322.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 3.86e-72 212
MS.gene78322.t1 MTR_2g082510 86.777 121 12 1 10 126 3 123 3.42e-69 204
MS.gene78322.t1 MTR_2g082220 84.921 126 11 2 10 129 3 126 4.30e-69 204
MS.gene78322.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 1.15e-59 181
MS.gene78322.t1 MTR_7g113160 76.068 117 28 0 9 125 16 132 3.78e-59 179
MS.gene78322.t1 MTR_4g063280 79.091 110 23 0 18 127 17 126 2.93e-58 177
MS.gene78322.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 5.36e-58 177
MS.gene78322.t1 MTR_4g063410 79.439 107 22 0 19 125 24 130 5.44e-58 177
MS.gene78322.t1 MTR_4g071150 80.374 107 21 0 19 125 24 130 6.46e-58 177
MS.gene78322.t1 MTR_4g064005 81.132 106 20 0 19 124 24 129 6.49e-58 177
MS.gene78322.t1 MTR_8g086640 81.132 106 20 0 19 124 24 129 7.30e-58 177
MS.gene78322.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 8.16e-58 176
MS.gene78322.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 5.87e-42 136
MS.gene78322.t1 MTR_1g011750 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene78322.t1 MTR_1g011850 58.197 122 49 2 3 123 10 130 1.06e-39 130
MS.gene78322.t1 MTR_1g114050 62.857 105 37 2 20 123 24 127 5.35e-38 125
MS.gene78322.t1 MTR_1g071040 63.462 104 36 2 21 123 28 130 1.15e-37 125
MS.gene78322.t1 MTR_4g063300 67.708 96 23 1 40 127 10 105 7.05e-36 120
MS.gene78322.t1 MTR_4g005910 88.889 45 5 0 82 126 4 48 6.44e-23 85.5
MS.gene78322.t1 MTR_7g010440 54.444 90 41 0 47 136 96 185 2.92e-22 89.7
MS.gene78322.t1 MTR_4g048490 44.860 107 30 2 19 125 26 103 2.83e-20 80.1
MS.gene78322.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 1.19e-18 75.9
MS.gene78322.t1 MTR_7g027610 41.818 110 32 3 18 123 13 94 8.02e-16 68.9
MS.gene78322.t1 MTR_0653s0030 52.830 53 25 0 50 102 43 95 4.63e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78322.t1 AT1G54690 92.958 142 7 1 1 139 1 142 2.22e-90 258
MS.gene78322.t1 AT1G08880 94.118 136 8 0 4 139 7 142 5.41e-90 258
MS.gene78322.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene78322.t1 AT4G27230 86.290 124 13 1 10 129 3 126 4.66e-70 207
MS.gene78322.t1 AT5G54640 82.946 129 19 1 1 129 1 126 1.59e-69 205
MS.gene78322.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.51e-68 203
MS.gene78322.t1 AT3G20670 84.677 124 15 1 10 129 3 126 4.76e-68 202
MS.gene78322.t1 AT5G59870 73.643 129 29 2 1 124 1 129 1.24e-60 184
MS.gene78322.t1 AT5G27670 73.438 128 29 1 2 124 3 130 4.78e-58 177
MS.gene78322.t1 AT5G02560 79.439 107 22 0 18 124 23 129 5.23e-57 174
MS.gene78322.t1 AT5G02560 64.885 131 22 1 18 124 23 153 1.37e-51 162
MS.gene78322.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78322.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78322.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78322.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.12e-38 127
MS.gene78322.t1 AT1G52740 61.905 105 38 2 20 123 27 130 2.42e-38 127
MS.gene78322.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene78322.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.94e-37 123
MS.gene78322.t1 AT4G13570 43.220 118 65 2 1 117 2 118 3.98e-23 87.8
MS.gene78322.t1 AT4G13570 49.451 91 44 2 28 117 35 124 9.39e-22 84.3

Find 49 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCCTTTCCAACCTTCTT+TGG 0.128920 7.4:-2877669 None:intergenic
TGCCCTTTCCAACCTTCTTT+GGG 0.261985 7.4:-2877668 None:intergenic
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.4:+2877590 MS.gene78322:CDS
TGGTTTGCCTCTTCCACCTT+TGG 0.309774 7.4:-2877165 None:intergenic
AACACGTTCGGCGTATTTAC+CGG 0.321195 7.4:-2877267 None:intergenic
GGAAAGGGCAAAGGAGAAAT+TGG 0.326882 7.4:+2877681 MS.gene78322:CDS
TTTACCGGCCTTGAGGAATC+TGG 0.369624 7.4:-2877252 None:intergenic
AAGACCTGCCTTTGAAGATC+TGG 0.371894 7.4:-2877207 None:intergenic
AGCAAAGTCTGATGAATATT+AGG 0.375759 7.4:-2877645 None:intergenic
TGAAGATCTGGAAACCGATT+TGG 0.392248 7.4:-2877195 None:intergenic
GAAACCGATTTGGCAGCCTT+TGG 0.410658 7.4:-2877185 None:intergenic
TCCACCTTTGGTTGTTCCAG+TGG 0.430840 7.4:-2877153 None:intergenic
CAAACCAAAGGCTGCCAAAT+CGG 0.434148 7.4:+2877181 MS.gene78322:CDS
GGCACATTCAGCTTGCTGTT+AGG 0.440630 7.4:+2877562 MS.gene78322:CDS
TCAGACTTTGCTCCCAAAGA+AGG 0.448013 7.4:+2877656 MS.gene78322:CDS
AGCTCCGGCACCAACACGTT+CGG 0.461862 7.4:-2877279 None:intergenic
AGGACGGCGGAGAGGTAGAC+AGG 0.463287 7.4:-2877302 None:intergenic
CAGCAAGCTGAATGTGCCTT+GGG 0.478109 7.4:-2877557 None:intergenic
GGAACTGTGACCATTGCTAA+TGG 0.483635 7.4:+2877612 MS.gene78322:CDS
CTCCCAAAGAAGGTTGGAAA+GGG 0.488096 7.4:+2877666 MS.gene78322:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.510267 7.4:-2877558 None:intergenic
GTTTCCAGATCTTCAAAGGC+AGG 0.515636 7.4:+2877203 MS.gene78322:CDS
GATCAGAAAATGAGCTCCAC+TGG 0.517370 7.4:+2877137 None:intergenic
ACTTTGCTCCCAAAGAAGGT+TGG 0.520151 7.4:+2877660 MS.gene78322:CDS
GGAACAACCAAAGGTGGAAG+AGG 0.533476 7.4:+2877158 MS.gene78322:CDS
TACGCCGAACGTGTTGGTGC+CGG 0.543181 7.4:+2877275 MS.gene78322:CDS
ATCGGTTTCCAGATCTTCAA+AGG 0.543380 7.4:+2877199 MS.gene78322:CDS
ATCGCCAGATTCCTCAAGGC+CGG 0.565542 7.4:+2877248 MS.gene78322:CDS
GCTCCCAAAGAAGGTTGGAA+AGG 0.567735 7.4:+2877665 MS.gene78322:CDS
CGGCGTATTTACCGGCCTTG+AGG 0.577173 7.4:-2877259 None:intergenic
GAATCTGGCGATTCTTCCGA+CGG 0.592664 7.4:-2877237 None:intergenic
GGTAAATACGCCGAACGTGT+TGG 0.593895 7.4:+2877269 MS.gene78322:CDS
GGAGAGGTAGACAGGAGCTC+CGG 0.601992 7.4:-2877294 None:intergenic
AGCTCCACTGGAACAACCAA+AGG 0.603686 7.4:+2877149 MS.gene78322:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.612329 7.4:+2877322 MS.gene78322:CDS
AAGAAGGTTGGAAAGGGCAA+AGG 0.616375 7.4:+2877672 MS.gene78322:CDS
AAGAATCGCCAGATTCCTCA+AGG 0.631643 7.4:+2877244 MS.gene78322:CDS
TCCACTGGAACAACCAAAGG+TGG 0.637545 7.4:+2877152 MS.gene78322:CDS
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.4:-2877322 None:intergenic
GAAACCCTAAAATTCTTGAG+AGG 0.640292 7.4:-2877709 None:intergenic
CAGAACACCACCATTAGCAA+TGG 0.646082 7.4:-2877622 None:intergenic
AGGTGGAAGAGGCAAACCAA+AGG 0.648004 7.4:+2877169 MS.gene78322:CDS
AGAAGAATCGTATTGTCCCA+AGG 0.648823 7.4:+2877541 MS.gene78322:CDS
AATCTGGCGATTCTTCCGAC+GGG 0.649569 7.4:-2877236 None:intergenic
GCAGGTCTTCAATTTCCCGT+CGG 0.653866 7.4:+2877221 MS.gene78322:CDS
GATACTCAAGGACGGCGGAG+AGG 0.669935 7.4:-2877310 None:intergenic
AGCAGCGAGATACTCAAGGA+CGG 0.684917 7.4:-2877318 None:intergenic
ACTGTGACCATTGCTAATGG+TGG 0.694716 7.4:+2877615 MS.gene78322:CDS
AGCGAGATACTCAAGGACGG+CGG 0.736224 7.4:-2877315 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATCTTTTTTGTTTAATTTA+GGG + chr7.4:2877375-2877394 MS.gene78322:intron 10.0%
!!! CTATCTTTTTTGTTTAATTT+AGG + chr7.4:2877374-2877393 MS.gene78322:intron 15.0%
!! ATATGAATCTGATAATTGAA+AGG + chr7.4:2877444-2877463 MS.gene78322:intron 20.0%
!! TAAACAAAAAAGATAGTGAA+AGG - chr7.4:2877370-2877389 None:intergenic 20.0%
!!! ATTTTTTGTTGATTAGGTTT+TGG + chr7.4:2877488-2877507 MS.gene78322:intron 20.0%
!!! CTGTTAATTTTTTGTTGATT+AGG + chr7.4:2877482-2877501 MS.gene78322:intron 20.0%
!!! GAATTGTCTTTGTTTTATTA+GGG + chr7.4:2877417-2877436 MS.gene78322:intron 20.0%
! AAAGATAGTGAAAGGAAAAT+TGG - chr7.4:2877362-2877381 None:intergenic 25.0%
!!! CGAATTGTCTTTGTTTTATT+AGG + chr7.4:2877416-2877435 MS.gene78322:intron 25.0%
AGCAAAGTCTGATGAATATT+AGG - chr7.4:2877648-2877667 None:intergenic 30.0%
CAAAGACAATTCGATTGAAT+TGG - chr7.4:2877409-2877428 None:intergenic 30.0%
!!! TGTTGATTAGGTTTTGGAAT+TGG + chr7.4:2877494-2877513 MS.gene78322:intron 30.0%
AGAAGAATCGTATTGTCCCA+AGG + chr7.4:2877541-2877560 MS.gene78322:CDS 40.0%
TGAAGATCTGGAAACCGATT+TGG - chr7.4:2877198-2877217 None:intergenic 40.0%
! ATCGGTTTCCAGATCTTCAA+AGG + chr7.4:2877199-2877218 MS.gene78322:CDS 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7.4:2877591-2877610 MS.gene78322:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7.4:2877590-2877609 MS.gene78322:CDS 40.0%
!!! GATTAGGTTTTGGAATTGGC+TGG + chr7.4:2877498-2877517 MS.gene78322:intron 40.0%
AACACGTTCGGCGTATTTAC+CGG - chr7.4:2877270-2877289 None:intergenic 45.0%
AAGAAGGTTGGAAAGGGCAA+AGG + chr7.4:2877672-2877691 MS.gene78322:CDS 45.0%
AAGAATCGCCAGATTCCTCA+AGG + chr7.4:2877244-2877263 MS.gene78322:CDS 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7.4:2877615-2877634 MS.gene78322:CDS 45.0%
ACTTTGCTCCCAAAGAAGGT+TGG + chr7.4:2877660-2877679 MS.gene78322:CDS 45.0%
CAAACCAAAGGCTGCCAAAT+CGG + chr7.4:2877181-2877200 MS.gene78322:CDS 45.0%
CAGAACACCACCATTAGCAA+TGG - chr7.4:2877625-2877644 None:intergenic 45.0%
CTCCCAAAGAAGGTTGGAAA+GGG + chr7.4:2877666-2877685 MS.gene78322:CDS 45.0%
GGAAAGGGCAAAGGAGAAAT+TGG + chr7.4:2877681-2877700 MS.gene78322:CDS 45.0%
GGAACTGTGACCATTGCTAA+TGG + chr7.4:2877612-2877631 MS.gene78322:CDS 45.0%
GTTTCCAGATCTTCAAAGGC+AGG + chr7.4:2877203-2877222 MS.gene78322:CDS 45.0%
TCAGACTTTGCTCCCAAAGA+AGG + chr7.4:2877656-2877675 MS.gene78322:CDS 45.0%
TGCCCTTTCCAACCTTCTTT+GGG - chr7.4:2877671-2877690 None:intergenic 45.0%
TTGCCCTTTCCAACCTTCTT+TGG - chr7.4:2877672-2877691 None:intergenic 45.0%
! AAGACCTGCCTTTGAAGATC+TGG - chr7.4:2877210-2877229 None:intergenic 45.0%
AATCTGGCGATTCTTCCGAC+GGG - chr7.4:2877239-2877258 None:intergenic 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7.4:2877561-2877580 None:intergenic 50.0%
AGCAGCGAGATACTCAAGGA+CGG - chr7.4:2877321-2877340 None:intergenic 50.0%
AGCTCCACTGGAACAACCAA+AGG + chr7.4:2877149-2877168 MS.gene78322:CDS 50.0%
AGGTGGAAGAGGCAAACCAA+AGG + chr7.4:2877169-2877188 MS.gene78322:CDS 50.0%
CAGCAAGCTGAATGTGCCTT+GGG - chr7.4:2877560-2877579 None:intergenic 50.0%
GAATCTGGCGATTCTTCCGA+CGG - chr7.4:2877240-2877259 None:intergenic 50.0%
GCAGGTCTTCAATTTCCCGT+CGG + chr7.4:2877221-2877240 MS.gene78322:CDS 50.0%
GCTCCCAAAGAAGGTTGGAA+AGG + chr7.4:2877665-2877684 MS.gene78322:CDS 50.0%
GGAACAACCAAAGGTGGAAG+AGG + chr7.4:2877158-2877177 MS.gene78322:CDS 50.0%
GGTAAATACGCCGAACGTGT+TGG + chr7.4:2877269-2877288 MS.gene78322:CDS 50.0%
TCCACCTTTGGTTGTTCCAG+TGG - chr7.4:2877156-2877175 None:intergenic 50.0%
TCCACTGGAACAACCAAAGG+TGG + chr7.4:2877152-2877171 MS.gene78322:CDS 50.0%
TTTACCGGCCTTGAGGAATC+TGG - chr7.4:2877255-2877274 None:intergenic 50.0%
! TGGTTTGCCTCTTCCACCTT+TGG - chr7.4:2877168-2877187 None:intergenic 50.0%
!! GAAACCGATTTGGCAGCCTT+TGG - chr7.4:2877188-2877207 None:intergenic 50.0%
!! GGCACATTCAGCTTGCTGTT+AGG + chr7.4:2877562-2877581 MS.gene78322:CDS 50.0%
AGCGAGATACTCAAGGACGG+CGG - chr7.4:2877318-2877337 None:intergenic 55.0%
ATCGCCAGATTCCTCAAGGC+CGG + chr7.4:2877248-2877267 MS.gene78322:CDS 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7.4:2877325-2877344 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7.4:2877322-2877341 MS.gene78322:CDS 55.0%
AGCTCCGGCACCAACACGTT+CGG - chr7.4:2877282-2877301 None:intergenic 60.0%
CGGCGTATTTACCGGCCTTG+AGG - chr7.4:2877262-2877281 None:intergenic 60.0%
GATACTCAAGGACGGCGGAG+AGG - chr7.4:2877313-2877332 None:intergenic 60.0%
GGAGAGGTAGACAGGAGCTC+CGG - chr7.4:2877297-2877316 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7.4:2877275-2877294 MS.gene78322:CDS 60.0%
AGGACGGCGGAGAGGTAGAC+AGG - chr7.4:2877305-2877324 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 2877146 2877725 2877146 ID=MS.gene78322
chr7.4 mRNA 2877146 2877725 2877146 ID=MS.gene78322.t1;Parent=MS.gene78322
chr7.4 exon 2877146 2877343 2877146 ID=MS.gene78322.t1.exon1;Parent=MS.gene78322.t1
chr7.4 CDS 2877146 2877343 2877146 ID=cds.MS.gene78322.t1;Parent=MS.gene78322.t1
chr7.4 exon 2877504 2877725 2877504 ID=MS.gene78322.t1.exon2;Parent=MS.gene78322.t1
chr7.4 CDS 2877504 2877725 2877504 ID=cds.MS.gene78322.t1;Parent=MS.gene78322.t1
Gene Sequence

>MS.gene78322

ATGAGCTCCACTGGAACAACCAAAGGTGGAAGAGGCAAACCAAAGGCTGCCAAATCGGTTTCCAGATCTTCAAAGGCAGGTCTTCAATTTCCCGTCGGAAGAATCGCCAGATTCCTCAAGGCCGGTAAATACGCCGAACGTGTTGGTGCCGGAGCTCCTGTCTACCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAGGTTTGTTTCTTTTCTCCAATTTTCCTTTCACTATCTTTTTTGTTTAATTTAGGGTTCTAGTTCCAATTCAATCGAATTGTCTTTGTTTTATTAGGGTTTTATATGAATCTGATAATTGAAAGGTAGAAACTCAATTTTCTGTTAATTTTTTGTTGATTAGGTTTTGGAATTGGCTGGAAACGCTGCTAGAGACAACAAGAAGAATCGTATTGTCCCAAGGCACATTCAGCTTGCTGTTAGGAACGATGAAGAACTCAGCAAGCTTTTGGGAACTGTGACCATTGCTAATGGTGGTGTTCTGCCTAATATTCATCAGACTTTGCTCCCAAAGAAGGTTGGAAAGGGCAAAGGAGAAATTGGATCTGCCTCTCAAGAATTTTAG

Protein sequence

>MS.gene78322.t1

MSSTGTTKGGRGKPKAAKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF