AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29299


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29299.t1 MTR_7g114060 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene29299.t1 MTR_7g114040 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene29299.t1 MTR_7g108320 95.070 142 4 1 1 139 1 142 3.24e-92 263
MS.gene29299.t1 MTR_7g093910 85.401 137 20 0 3 139 6 142 4.01e-82 238
MS.gene29299.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 9.58e-73 213
MS.gene29299.t1 MTR_2g082510 87.603 121 11 1 10 126 3 123 6.39e-70 206
MS.gene29299.t1 MTR_2g082220 86.290 124 13 1 10 129 3 126 7.87e-70 206
MS.gene29299.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 7.95e-60 181
MS.gene29299.t1 MTR_7g113160 76.033 121 25 1 4 124 15 131 3.14e-59 180
MS.gene29299.t1 MTR_4g063280 72.441 127 34 1 1 127 1 126 2.43e-58 177
MS.gene29299.t1 MTR_8g086640 79.817 109 22 0 19 127 24 132 2.79e-58 177
MS.gene29299.t1 MTR_4g064005 79.817 109 22 0 19 127 24 132 4.57e-58 177
MS.gene29299.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 4.86e-58 177
MS.gene29299.t1 MTR_4g071150 81.132 106 20 0 19 124 24 129 6.90e-58 177
MS.gene29299.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 9.83e-58 176
MS.gene29299.t1 MTR_4g063410 80.189 106 21 0 19 124 24 129 1.90e-57 176
MS.gene29299.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 3.71e-42 136
MS.gene29299.t1 MTR_1g011750 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene29299.t1 MTR_1g011850 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene29299.t1 MTR_1g114050 62.617 107 38 2 20 125 24 129 1.90e-38 127
MS.gene29299.t1 MTR_1g071040 63.208 106 37 2 21 125 28 132 3.90e-38 126
MS.gene29299.t1 MTR_4g063300 79.730 74 15 0 40 113 10 83 9.89e-36 119
MS.gene29299.t1 MTR_4g005910 91.111 45 4 0 82 126 4 48 1.24e-23 87.0
MS.gene29299.t1 MTR_7g010440 69.697 66 20 0 73 138 122 187 5.68e-23 91.7
MS.gene29299.t1 MTR_4g048490 45.714 105 28 2 19 123 26 101 6.65e-20 79.0
MS.gene29299.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 6.85e-19 76.6
MS.gene29299.t1 MTR_7g027610 40.323 124 39 4 6 125 4 96 2.25e-16 70.1
MS.gene29299.t1 MTR_0653s0030 40.000 100 35 2 3 102 21 95 1.95e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29299.t1 AT1G54690 94.118 136 8 0 4 139 7 142 9.16e-90 257
MS.gene29299.t1 AT1G08880 93.382 136 9 0 4 139 7 142 2.28e-89 256
MS.gene29299.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene29299.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene29299.t1 AT5G54640 86.290 124 13 1 10 129 3 126 9.30e-70 206
MS.gene29299.t1 AT3G20670 85.484 124 14 1 10 129 3 126 1.04e-68 203
MS.gene29299.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.49e-68 203
MS.gene29299.t1 AT5G59870 72.308 130 31 2 1 125 1 130 1.56e-60 183
MS.gene29299.t1 AT5G27670 70.588 136 34 2 4 137 14 145 1.16e-57 176
MS.gene29299.t1 AT5G02560 78.182 110 24 0 18 127 23 132 1.81e-57 176
MS.gene29299.t1 AT5G02560 64.179 134 24 1 18 127 23 156 4.48e-52 163
MS.gene29299.t1 AT1G52740 57.377 122 43 3 12 125 12 132 6.98e-39 128
MS.gene29299.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene29299.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene29299.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene29299.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene29299.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene29299.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene29299.t1 AT4G13570 45.045 111 59 2 8 117 9 118 2.09e-22 85.9
MS.gene29299.t1 AT4G13570 49.451 91 44 2 28 117 35 124 8.80e-22 84.3

Find 51 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCCTTCCCAGCCTTCTT+TGG 0.272919 7.2:-3356036 None:intergenic
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.2:+3355957 MS.gene29299:CDS
TGAAGATCTGGAAACTGATT+TGG 0.302821 7.2:-3355633 None:intergenic
CAGCAAAGTCTGATGAATAT+TGG 0.310312 7.2:-3356013 None:intergenic
GGGAAGGGAAAAGGCGAAAT+TGG 0.311073 7.2:+3356048 MS.gene29299:CDS
AACACGTTCGGCGTATTTGC+CGG 0.318153 7.2:-3355705 None:intergenic
AAGTCCGGCCTTTGAAGATC+TGG 0.387108 7.2:-3355645 None:intergenic
AGCAAAGTCTGATGAATATT+GGG 0.399419 7.2:-3356012 None:intergenic
GAAACTGATTTGGAAGCCTT+CGG 0.412102 7.2:-3355623 None:intergenic
ACTTTGCTGCCAAAGAAGGC+TGG 0.424753 7.2:+3356027 MS.gene29299:CDS
TCAGACTTTGCTGCCAAAGA+AGG 0.433123 7.2:+3356023 MS.gene29299:CDS
AGCTCCGGCACCAACACGTT+CGG 0.444935 7.2:-3355717 None:intergenic
AAGAAGGCTGGGAAGGGAAA+AGG 0.471306 7.2:+3356039 MS.gene29299:CDS
CTGGTACCACCAAGGGAGGA+AGG 0.482850 7.2:+3355594 MS.gene29299:CDS
GGAACTGTGACCATTGCTAA+TGG 0.483635 7.2:+3355979 MS.gene29299:CDS
ACGTGTTGGTGCCGGAGCTC+CGG 0.485122 7.2:+3355721 MS.gene29299:CDS
GGCACATTCAGCTTGCTGTC+AGG 0.491110 7.2:+3355929 MS.gene29299:CDS
CGGCGTATTTGCCGGCCTTG+AGG 0.502918 7.2:-3355697 None:intergenic
GGTACCACCAAGGGAGGAAG+GGG 0.504666 7.2:+3355596 MS.gene29299:CDS
CTTTGCTGCCAAAGAAGGCT+GGG 0.518155 7.2:+3356028 MS.gene29299:CDS
TGGTACCACCAAGGGAGGAA+GGG 0.518374 7.2:+3355595 MS.gene29299:CDS
GTTTCCAGATCTTCAAAGGC+CGG 0.518477 7.2:+3355641 MS.gene29299:CDS
TCCGACGGGGAATTGAAGTC+CGG 0.524129 7.2:-3355660 None:intergenic
TACGCCGAACGTGTTGGTGC+CGG 0.525825 7.2:+3355713 MS.gene29299:CDS
GCTGCCAAAGAAGGCTGGGA+AGG 0.531759 7.2:+3356032 MS.gene29299:CDS
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7.2:+3355878 MS.gene29299:CDS
ATCGCAAGATTCCTCAAGGC+CGG 0.538535 7.2:+3355686 MS.gene29299:CDS
ATCAGTTTCCAGATCTTCAA+AGG 0.543380 7.2:+3355637 MS.gene29299:CDS
AGAGAGGTATACCGGAGCTC+CGG 0.548996 7.2:-3355732 None:intergenic
AGGACCGCAGAGAGGTATAC+CGG 0.559095 7.2:-3355740 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.559881 7.2:-3355674 None:intergenic
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7.2:+3355879 MS.gene29299:CDS
GATTCTGCTGGTACCACCAA+GGG 0.568843 7.2:+3355587 MS.gene29299:CDS
GAATCTTGCGATTCTTCCGA+CGG 0.569168 7.2:-3355675 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7.2:-3355915 None:intergenic
CTGCCAAAGAAGGCTGGGAA+GGG 0.583937 7.2:+3356033 MS.gene29299:CDS
GGCAAATACGCCGAACGTGT+TGG 0.584120 7.2:+3355707 MS.gene29299:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.605047 7.2:-3355925 None:intergenic
AAGAATCGCAAGATTCCTCA+AGG 0.619754 7.2:+3355682 MS.gene29299:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.632170 7.2:+3355760 MS.gene29299:CDS
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.2:-3355760 None:intergenic
TCTGCTGGTACCACCAAGGG+AGG 0.639099 7.2:+3355590 MS.gene29299:CDS
CAGAACACCACCATTAGCAA+TGG 0.646082 7.2:-3355989 None:intergenic
GCCGGACTTCAATTCCCCGT+CGG 0.658042 7.2:+3355659 MS.gene29299:CDS
GGATTCTGCTGGTACCACCA+AGG 0.659312 7.2:+3355586 MS.gene29299:CDS
GTACCACCAAGGGAGGAAGG+GGG 0.685414 7.2:+3355597 MS.gene29299:CDS
AGAAGAACCGTATTGTACCA+AGG 0.686303 7.2:+3355908 MS.gene29299:CDS
ACTGTGACCATTGCTAATGG+TGG 0.694716 7.2:+3355982 MS.gene29299:CDS
ATCTTGCGATTCTTCCGACG+GGG 0.695192 7.2:-3355673 None:intergenic
GATACTCAAGGACCGCAGAG+AGG 0.712819 7.2:-3355748 None:intergenic
AGCTCCGGTATACCTCTCTG+CGG 0.763589 7.2:+3355736 MS.gene29299:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAACTGTATTTTAATTT+TGG + chr7.2:3355840-3355859 MS.gene29299:intron 10.0%
!!! TTAAACTGTATTTTAATTTT+GGG + chr7.2:3355841-3355860 MS.gene29299:intron 10.0%
!! AAAATACAGTTTAATCAAAT+TGG - chr7.2:3355835-3355854 None:intergenic 15.0%
!!! TATTTTAATTTTGGGTATTT+AGG + chr7.2:3355849-3355868 MS.gene29299:intron 15.0%
! ATACAAAAAAGAGAGTGAAA+GGG - chr7.2:3355807-3355826 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG - chr7.2:3355808-3355827 None:intergenic 25.0%
!!! AATTTTGGGTATTTAGGTTT+TGG + chr7.2:3355855-3355874 MS.gene29299:intron 25.0%
AGCAAAGTCTGATGAATATT+GGG - chr7.2:3356015-3356034 None:intergenic 30.0%
ATCAGTTTCCAGATCTTCAA+AGG + chr7.2:3355637-3355656 MS.gene29299:CDS 35.0%
CAGCAAAGTCTGATGAATAT+TGG - chr7.2:3356016-3356035 None:intergenic 35.0%
TGAAGATCTGGAAACTGATT+TGG - chr7.2:3355636-3355655 None:intergenic 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG + chr7.2:3355865-3355884 MS.gene29299:intron 35.0%
!!! GGGTATTTAGGTTTTGGAAT+TGG + chr7.2:3355861-3355880 MS.gene29299:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG + chr7.2:3355866-3355885 MS.gene29299:intron 35.0%
AAGAATCGCAAGATTCCTCA+AGG + chr7.2:3355682-3355701 MS.gene29299:CDS 40.0%
AGAAGAACCGTATTGTACCA+AGG + chr7.2:3355908-3355927 MS.gene29299:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG - chr7.2:3355918-3355937 None:intergenic 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7.2:3355958-3355977 MS.gene29299:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7.2:3355957-3355976 MS.gene29299:CDS 40.0%
!! GAAACTGATTTGGAAGCCTT+CGG - chr7.2:3355626-3355645 None:intergenic 40.0%
AATCTTGCGATTCTTCCGAC+GGG - chr7.2:3355677-3355696 None:intergenic 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7.2:3355982-3356001 MS.gene29299:CDS 45.0%
CAGAACACCACCATTAGCAA+TGG - chr7.2:3355992-3356011 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG - chr7.2:3355678-3355697 None:intergenic 45.0%
GGAACTGTGACCATTGCTAA+TGG + chr7.2:3355979-3355998 MS.gene29299:CDS 45.0%
GTTTCCAGATCTTCAAAGGC+CGG + chr7.2:3355641-3355660 MS.gene29299:CDS 45.0%
TCAGACTTTGCTGCCAAAGA+AGG + chr7.2:3356023-3356042 MS.gene29299:CDS 45.0%
AACACGTTCGGCGTATTTGC+CGG - chr7.2:3355708-3355727 None:intergenic 50.0%
AAGAAGGCTGGGAAGGGAAA+AGG + chr7.2:3356039-3356058 MS.gene29299:CDS 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7.2:3355928-3355947 None:intergenic 50.0%
ACTTTGCTGCCAAAGAAGGC+TGG + chr7.2:3356027-3356046 MS.gene29299:CDS 50.0%
ATCGCAAGATTCCTCAAGGC+CGG + chr7.2:3355686-3355705 MS.gene29299:CDS 50.0%
ATCTTGCGATTCTTCCGACG+GGG - chr7.2:3355676-3355695 None:intergenic 50.0%
CTTTGCTGCCAAAGAAGGCT+GGG + chr7.2:3356028-3356047 MS.gene29299:CDS 50.0%
GATTCTGCTGGTACCACCAA+GGG + chr7.2:3355587-3355606 MS.gene29299:CDS 50.0%
GGGAAGGGAAAAGGCGAAAT+TGG + chr7.2:3356048-3356067 MS.gene29299:CDS 50.0%
TTTCCCTTCCCAGCCTTCTT+TGG - chr7.2:3356039-3356058 None:intergenic 50.0%
! AAGTCCGGCCTTTGAAGATC+TGG - chr7.2:3355648-3355667 None:intergenic 50.0%
AATTGGCTGGGAACGCTGCA+AGG + chr7.2:3355878-3355897 MS.gene29299:CDS 55.0%
AGCTCCGGTATACCTCTCTG+CGG + chr7.2:3355736-3355755 MS.gene29299:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG + chr7.2:3355879-3355898 MS.gene29299:CDS 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7.2:3355763-3355782 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7.2:3355760-3355779 MS.gene29299:CDS 55.0%
CTGCCAAAGAAGGCTGGGAA+GGG + chr7.2:3356033-3356052 MS.gene29299:CDS 55.0%
GATACTCAAGGACCGCAGAG+AGG - chr7.2:3355751-3355770 None:intergenic 55.0%
GGATTCTGCTGGTACCACCA+AGG + chr7.2:3355586-3355605 MS.gene29299:CDS 55.0%
GGCAAATACGCCGAACGTGT+TGG + chr7.2:3355707-3355726 MS.gene29299:CDS 55.0%
TCCGACGGGGAATTGAAGTC+CGG - chr7.2:3355663-3355682 None:intergenic 55.0%
TGGTACCACCAAGGGAGGAA+GGG + chr7.2:3355595-3355614 MS.gene29299:CDS 55.0%
! AGAGAGGTATACCGGAGCTC+CGG - chr7.2:3355735-3355754 None:intergenic 55.0%
! AGGACCGCAGAGAGGTATAC+CGG - chr7.2:3355743-3355762 None:intergenic 55.0%
!! GGCACATTCAGCTTGCTGTC+AGG + chr7.2:3355929-3355948 MS.gene29299:CDS 55.0%
AGCTCCGGCACCAACACGTT+CGG - chr7.2:3355720-3355739 None:intergenic 60.0%
CTGGTACCACCAAGGGAGGA+AGG + chr7.2:3355594-3355613 MS.gene29299:CDS 60.0%
GCCGGACTTCAATTCCCCGT+CGG + chr7.2:3355659-3355678 MS.gene29299:CDS 60.0%
GCTGCCAAAGAAGGCTGGGA+AGG + chr7.2:3356032-3356051 MS.gene29299:CDS 60.0%
GGTACCACCAAGGGAGGAAG+GGG + chr7.2:3355596-3355615 MS.gene29299:CDS 60.0%
GTACCACCAAGGGAGGAAGG+GGG + chr7.2:3355597-3355616 MS.gene29299:CDS 60.0%
TCTGCTGGTACCACCAAGGG+AGG + chr7.2:3355590-3355609 MS.gene29299:CDS 60.0%
TTTCCCCCTTCCTCCCTTGG+TGG - chr7.2:3355603-3355622 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7.2:3355713-3355732 MS.gene29299:CDS 60.0%
CGGCGTATTTGCCGGCCTTG+AGG - chr7.2:3355700-3355719 None:intergenic 65.0%
CGGTTTCCCCCTTCCTCCCT+TGG - chr7.2:3355606-3355625 None:intergenic 65.0%
GGGAGGAAGGGGGAAACCGA+AGG + chr7.2:3355607-3355626 MS.gene29299:CDS 65.0%
!! ACGTGTTGGTGCCGGAGCTC+CGG + chr7.2:3355721-3355740 MS.gene29299:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 3355584 3356092 3355584 ID=MS.gene29299
chr7.2 mRNA 3355584 3356092 3355584 ID=MS.gene29299.t1;Parent=MS.gene29299
chr7.2 exon 3355584 3355781 3355584 ID=MS.gene29299.t1.exon1;Parent=MS.gene29299.t1
chr7.2 CDS 3355584 3355781 3355584 ID=cds.MS.gene29299.t1;Parent=MS.gene29299.t1
chr7.2 exon 3355871 3356092 3355871 ID=MS.gene29299.t1.exon2;Parent=MS.gene29299.t1
chr7.2 CDS 3355871 3356092 3355871 ID=cds.MS.gene29299.t1;Parent=MS.gene29299.t1
Gene Sequence

>MS.gene29299

ATGGATTCTGCTGGTACCACCAAGGGAGGAAGGGGGAAACCGAAGGCTTCCAAATCAGTTTCCAGATCTTCAAAGGCCGGACTTCAATTCCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCCGGCAAATACGCCGAACGTGTTGGTGCCGGAGCTCCGGTATACCTCTCTGCGGTCCTTGAGTATCTCGCTGCTGAGGTTCGTTTCTTTTCTTCAATTTCCCTTTCACTCTCTTTTTTGTATAATATCCAATTTGATTAAACTGTATTTTAATTTTGGGTATTTAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTCAGGAACGATGAAGAACTCAGCAAGCTTTTGGGAACTGTGACCATTGCTAATGGTGGTGTTCTGCCCAATATTCATCAGACTTTGCTGCCAAAGAAGGCTGGGAAGGGAAAAGGCGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>MS.gene29299.t1

MDSAGTTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKAGKGKGEIGSASQEF