AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066536


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066536.t1 MTR_7g114060 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene066536.t1 MTR_7g114040 97.122 139 4 0 1 139 1 139 1.04e-94 269
MS.gene066536.t1 MTR_7g108320 95.070 142 4 1 1 139 1 142 3.24e-92 263
MS.gene066536.t1 MTR_7g093910 85.401 137 20 0 3 139 6 142 4.01e-82 238
MS.gene066536.t1 MTR_7g114050 87.023 131 6 2 9 139 11 130 9.58e-73 213
MS.gene066536.t1 MTR_2g082510 87.603 121 11 1 10 126 3 123 6.39e-70 206
MS.gene066536.t1 MTR_2g082220 86.290 124 13 1 10 129 3 126 7.87e-70 206
MS.gene066536.t1 MTR_2g096610 75.000 120 30 0 18 137 21 140 7.95e-60 181
MS.gene066536.t1 MTR_7g113160 76.033 121 25 1 4 124 15 131 3.14e-59 180
MS.gene066536.t1 MTR_4g063280 72.441 127 34 1 1 127 1 126 2.43e-58 177
MS.gene066536.t1 MTR_8g086640 79.817 109 22 0 19 127 24 132 2.79e-58 177
MS.gene066536.t1 MTR_4g064005 79.817 109 22 0 19 127 24 132 4.57e-58 177
MS.gene066536.t1 MTR_2g096570 81.132 106 20 0 19 124 23 128 4.86e-58 177
MS.gene066536.t1 MTR_4g071150 81.132 106 20 0 19 124 24 129 6.90e-58 177
MS.gene066536.t1 MTR_4g064967 81.132 106 20 0 19 124 24 129 9.83e-58 176
MS.gene066536.t1 MTR_4g063410 80.189 106 21 0 19 124 24 129 1.90e-57 176
MS.gene066536.t1 MTR_6g005150 61.345 119 37 2 19 136 26 136 3.71e-42 136
MS.gene066536.t1 MTR_1g011750 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene066536.t1 MTR_1g011850 63.551 107 37 2 18 123 25 130 1.59e-39 129
MS.gene066536.t1 MTR_1g114050 62.617 107 38 2 20 125 24 129 1.90e-38 127
MS.gene066536.t1 MTR_1g071040 63.208 106 37 2 21 125 28 132 3.90e-38 126
MS.gene066536.t1 MTR_4g063300 79.730 74 15 0 40 113 10 83 9.89e-36 119
MS.gene066536.t1 MTR_4g005910 91.111 45 4 0 82 126 4 48 1.24e-23 87.0
MS.gene066536.t1 MTR_7g010440 69.697 66 20 0 73 138 122 187 5.68e-23 91.7
MS.gene066536.t1 MTR_4g048490 45.714 105 28 2 19 123 26 101 6.65e-20 79.0
MS.gene066536.t1 MTR_1g069770 55.224 67 30 0 62 128 32 98 6.85e-19 76.6
MS.gene066536.t1 MTR_7g027610 40.323 124 39 4 6 125 4 96 2.25e-16 70.1
MS.gene066536.t1 MTR_0653s0030 40.000 100 35 2 3 102 21 95 1.95e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066536.t1 AT1G54690 94.118 136 8 0 4 139 7 142 9.16e-90 257
MS.gene066536.t1 AT1G08880 93.382 136 9 0 4 139 7 142 2.28e-89 256
MS.gene066536.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene066536.t1 AT4G27230 87.097 124 12 1 10 129 3 126 9.93e-71 208
MS.gene066536.t1 AT5G54640 86.290 124 13 1 10 129 3 126 9.30e-70 206
MS.gene066536.t1 AT3G20670 85.484 124 14 1 10 129 3 126 1.04e-68 203
MS.gene066536.t1 AT1G51060 83.871 124 16 1 10 129 3 126 1.49e-68 203
MS.gene066536.t1 AT5G59870 72.308 130 31 2 1 125 1 130 1.56e-60 183
MS.gene066536.t1 AT5G27670 70.588 136 34 2 4 137 14 145 1.16e-57 176
MS.gene066536.t1 AT5G02560 78.182 110 24 0 18 127 23 132 1.81e-57 176
MS.gene066536.t1 AT5G02560 64.179 134 24 1 18 127 23 156 4.48e-52 163
MS.gene066536.t1 AT1G52740 57.377 122 43 3 12 125 12 132 6.98e-39 128
MS.gene066536.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene066536.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene066536.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene066536.t1 AT2G38810 62.617 107 38 2 18 123 27 132 1.15e-38 127
MS.gene066536.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene066536.t1 AT3G54560 60.952 105 39 2 20 123 29 132 4.84e-37 123
MS.gene066536.t1 AT4G13570 45.045 111 59 2 8 117 9 118 2.09e-22 85.9
MS.gene066536.t1 AT4G13570 49.451 91 44 2 28 117 35 124 8.80e-22 84.3

Find 50 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCCTTCCCAGCCTTCTT+TGG 0.272919 7.1:-2484352 None:intergenic
TGAAGAACTCAGCAAGCTTT+TGG 0.286717 7.1:+2484273 MS.gene066536:CDS
TGAAGATCTCGAAACTGATT+TGG 0.308764 7.1:-2483937 None:intergenic
CAGCAAAGTCTGATGAATAT+TGG 0.310312 7.1:-2484329 None:intergenic
GGGAAGGGAAAAGGCGAAAT+TGG 0.311073 7.1:+2484364 MS.gene066536:CDS
AACACGTTCGGCGTATTTGC+CGG 0.318153 7.1:-2484009 None:intergenic
AGCAAAGTCTGATGAATATT+GGG 0.399419 7.1:-2484328 None:intergenic
GAAACTGATTTGGAAGCCTT+CGG 0.422557 7.1:-2483927 None:intergenic
ACTTTGCTGCCAAAGAAGGC+TGG 0.424753 7.1:+2484343 MS.gene066536:CDS
TCAGACTTTGCTGCCAAAGA+AGG 0.433123 7.1:+2484339 MS.gene066536:CDS
AGCTCCGGCACCAACACGTT+CGG 0.444935 7.1:-2484021 None:intergenic
AAGAAGGCTGGGAAGGGAAA+AGG 0.471306 7.1:+2484355 MS.gene066536:CDS
GGAACTGTGACCATTGCTAA+TGG 0.483635 7.1:+2484295 MS.gene066536:CDS
ACGTGTTGGTGCCGGAGCTC+CGG 0.485122 7.1:+2484025 MS.gene066536:CDS
GGCACATTCAGCTTGCTGTC+AGG 0.491110 7.1:+2484245 MS.gene066536:CDS
CGGCGTATTTGCCGGCCTTG+AGG 0.502918 7.1:-2484001 None:intergenic
CTGGTACCACCAAGGGAGGA+AGG 0.503740 7.1:+2483898 MS.gene066536:CDS
GGTACCACCAAGGGAGGAAG+GGG 0.506354 7.1:+2483900 MS.gene066536:CDS
CTTTGCTGCCAAAGAAGGCT+GGG 0.518155 7.1:+2484344 MS.gene066536:CDS
GTTTCGAGATCTTCAAAGGC+CGG 0.518477 7.1:+2483945 MS.gene066536:CDS
TCCGACGGGGAATTGAAGTC+CGG 0.524129 7.1:-2483964 None:intergenic
TACGCCGAACGTGTTGGTGC+CGG 0.525825 7.1:+2484017 MS.gene066536:CDS
GCTGCCAAAGAAGGCTGGGA+AGG 0.531759 7.1:+2484348 MS.gene066536:CDS
AATTGGCTGGGAACGCTGCA+AGG 0.533557 7.1:+2484194 MS.gene066536:CDS
ATCGCAAGATTCCTCAAGGC+CGG 0.538535 7.1:+2483990 MS.gene066536:CDS
TGGTACCACCAAGGGAGGAA+GGG 0.539265 7.1:+2483899 MS.gene066536:CDS
AGAGAGGTATACCGGAGCTC+CGG 0.548996 7.1:-2484036 None:intergenic
AGGACCGCAGAGAGGTATAC+CGG 0.559095 7.1:-2484044 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.559881 7.1:-2483978 None:intergenic
ATTGGCTGGGAACGCTGCAA+GGG 0.562499 7.1:+2484195 MS.gene066536:CDS
ATCAGTTTCGAGATCTTCAA+AGG 0.563708 7.1:+2483941 MS.gene066536:CDS
GATTCTGCTGGTACCACCAA+GGG 0.568843 7.1:+2483891 MS.gene066536:CDS
GAATCTTGCGATTCTTCCGA+CGG 0.569168 7.1:-2483979 None:intergenic
GAATGTGCCTTGGTACAATA+CGG 0.571461 7.1:-2484231 None:intergenic
CTGCCAAAGAAGGCTGGGAA+GGG 0.583937 7.1:+2484349 MS.gene066536:CDS
GGCAAATACGCCGAACGTGT+TGG 0.584120 7.1:+2484011 MS.gene066536:CDS
ACAGCAAGCTGAATGTGCCT+TGG 0.605047 7.1:-2484241 None:intergenic
GGGAGGAAGGGGAAAACCGA+AGG 0.611626 7.1:+2483911 MS.gene066536:CDS
AAGAATCGCAAGATTCCTCA+AGG 0.619754 7.1:+2483986 MS.gene066536:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.632170 7.1:+2484064 MS.gene066536:CDS
CCTCAGCAGCGAGATACTCA+AGG 0.638807 7.1:-2484064 None:intergenic
TCTGCTGGTACCACCAAGGG+AGG 0.639099 7.1:+2483894 MS.gene066536:CDS
CAGAACACCACCATTAGCAA+TGG 0.646082 7.1:-2484305 None:intergenic
GCCGGACTTCAATTCCCCGT+CGG 0.658042 7.1:+2483963 MS.gene066536:CDS
GGATTCTGCTGGTACCACCA+AGG 0.659312 7.1:+2483890 MS.gene066536:CDS
AGAAGAACCGTATTGTACCA+AGG 0.686303 7.1:+2484224 MS.gene066536:CDS
ACTGTGACCATTGCTAATGG+TGG 0.694716 7.1:+2484298 MS.gene066536:CDS
ATCTTGCGATTCTTCCGACG+GGG 0.695192 7.1:-2483977 None:intergenic
GATACTCAAGGACCGCAGAG+AGG 0.712819 7.1:-2484052 None:intergenic
AGCTCCGGTATACCTCTCTG+CGG 0.763589 7.1:+2484040 MS.gene066536:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAACTGTATTTTAATTT+TGG + chr7.1:2484156-2484175 MS.gene066536:intron 10.0%
!!! TTAAACTGTATTTTAATTTT+GGG + chr7.1:2484157-2484176 MS.gene066536:intron 10.0%
!! AAAATACAGTTTAATCAAAT+TGG - chr7.1:2484151-2484170 None:intergenic 15.0%
!!! TATTTTAATTTTGGGTATTT+AGG + chr7.1:2484165-2484184 MS.gene066536:intron 15.0%
!!! TCTCTTTTTTGTATAATTTA+GGG + chr7.1:2484117-2484136 MS.gene066536:intron 15.0%
!!! CTCTCTTTTTTGTATAATTT+AGG + chr7.1:2484116-2484135 MS.gene066536:intron 20.0%
! ATACAAAAAAGAGAGTGAAA+GGG - chr7.1:2484111-2484130 None:intergenic 25.0%
! TATACAAAAAAGAGAGTGAA+AGG - chr7.1:2484112-2484131 None:intergenic 25.0%
!!! AATTTTGGGTATTTAGGTTT+TGG + chr7.1:2484171-2484190 MS.gene066536:intron 25.0%
AGCAAAGTCTGATGAATATT+GGG - chr7.1:2484331-2484350 None:intergenic 30.0%
ATCAGTTTCGAGATCTTCAA+AGG + chr7.1:2483941-2483960 MS.gene066536:CDS 35.0%
CAGCAAAGTCTGATGAATAT+TGG - chr7.1:2484332-2484351 None:intergenic 35.0%
TGAAGATCTCGAAACTGATT+TGG - chr7.1:2483940-2483959 None:intergenic 35.0%
!!! ATTTAGGTTTTGGAATTGGC+TGG + chr7.1:2484181-2484200 MS.gene066536:intron 35.0%
!!! GGGTATTTAGGTTTTGGAAT+TGG + chr7.1:2484177-2484196 MS.gene066536:intron 35.0%
!!! TTTAGGTTTTGGAATTGGCT+GGG + chr7.1:2484182-2484201 MS.gene066536:intron 35.0%
AAGAATCGCAAGATTCCTCA+AGG + chr7.1:2483986-2484005 MS.gene066536:CDS 40.0%
AGAAGAACCGTATTGTACCA+AGG + chr7.1:2484224-2484243 MS.gene066536:CDS 40.0%
GAATGTGCCTTGGTACAATA+CGG - chr7.1:2484234-2484253 None:intergenic 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7.1:2484274-2484293 MS.gene066536:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7.1:2484273-2484292 MS.gene066536:CDS 40.0%
!! GAAACTGATTTGGAAGCCTT+CGG - chr7.1:2483930-2483949 None:intergenic 40.0%
AATCTTGCGATTCTTCCGAC+GGG - chr7.1:2483981-2484000 None:intergenic 45.0%
ACTGTGACCATTGCTAATGG+TGG + chr7.1:2484298-2484317 MS.gene066536:CDS 45.0%
CAGAACACCACCATTAGCAA+TGG - chr7.1:2484308-2484327 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG - chr7.1:2483982-2484001 None:intergenic 45.0%
GGAACTGTGACCATTGCTAA+TGG + chr7.1:2484295-2484314 MS.gene066536:CDS 45.0%
GTTTCGAGATCTTCAAAGGC+CGG + chr7.1:2483945-2483964 MS.gene066536:CDS 45.0%
TCAGACTTTGCTGCCAAAGA+AGG + chr7.1:2484339-2484358 MS.gene066536:CDS 45.0%
AACACGTTCGGCGTATTTGC+CGG - chr7.1:2484012-2484031 None:intergenic 50.0%
AAGAAGGCTGGGAAGGGAAA+AGG + chr7.1:2484355-2484374 MS.gene066536:CDS 50.0%
ACAGCAAGCTGAATGTGCCT+TGG - chr7.1:2484244-2484263 None:intergenic 50.0%
ACTTTGCTGCCAAAGAAGGC+TGG + chr7.1:2484343-2484362 MS.gene066536:CDS 50.0%
ATCGCAAGATTCCTCAAGGC+CGG + chr7.1:2483990-2484009 MS.gene066536:CDS 50.0%
ATCTTGCGATTCTTCCGACG+GGG - chr7.1:2483980-2483999 None:intergenic 50.0%
CTTTGCTGCCAAAGAAGGCT+GGG + chr7.1:2484344-2484363 MS.gene066536:CDS 50.0%
GATTCTGCTGGTACCACCAA+GGG + chr7.1:2483891-2483910 MS.gene066536:CDS 50.0%
GGGAAGGGAAAAGGCGAAAT+TGG + chr7.1:2484364-2484383 MS.gene066536:CDS 50.0%
TTTCCCTTCCCAGCCTTCTT+TGG - chr7.1:2484355-2484374 None:intergenic 50.0%
AATTGGCTGGGAACGCTGCA+AGG + chr7.1:2484194-2484213 MS.gene066536:CDS 55.0%
AGCTCCGGTATACCTCTCTG+CGG + chr7.1:2484040-2484059 MS.gene066536:CDS 55.0%
ATTGGCTGGGAACGCTGCAA+GGG + chr7.1:2484195-2484214 MS.gene066536:CDS 55.0%
CCTCAGCAGCGAGATACTCA+AGG - chr7.1:2484067-2484086 None:intergenic 55.0%
CCTTGAGTATCTCGCTGCTG+AGG + chr7.1:2484064-2484083 MS.gene066536:CDS 55.0%
CTGCCAAAGAAGGCTGGGAA+GGG + chr7.1:2484349-2484368 MS.gene066536:CDS 55.0%
GATACTCAAGGACCGCAGAG+AGG - chr7.1:2484055-2484074 None:intergenic 55.0%
GGATTCTGCTGGTACCACCA+AGG + chr7.1:2483890-2483909 MS.gene066536:CDS 55.0%
GGCAAATACGCCGAACGTGT+TGG + chr7.1:2484011-2484030 MS.gene066536:CDS 55.0%
TCCGACGGGGAATTGAAGTC+CGG - chr7.1:2483967-2483986 None:intergenic 55.0%
TGGTACCACCAAGGGAGGAA+GGG + chr7.1:2483899-2483918 MS.gene066536:CDS 55.0%
! AGAGAGGTATACCGGAGCTC+CGG - chr7.1:2484039-2484058 None:intergenic 55.0%
! AGGACCGCAGAGAGGTATAC+CGG - chr7.1:2484047-2484066 None:intergenic 55.0%
! TTTTCCCCTTCCTCCCTTGG+TGG - chr7.1:2483907-2483926 None:intergenic 55.0%
!! GGCACATTCAGCTTGCTGTC+AGG + chr7.1:2484245-2484264 MS.gene066536:CDS 55.0%
AGCTCCGGCACCAACACGTT+CGG - chr7.1:2484024-2484043 None:intergenic 60.0%
CTGGTACCACCAAGGGAGGA+AGG + chr7.1:2483898-2483917 MS.gene066536:CDS 60.0%
GCCGGACTTCAATTCCCCGT+CGG + chr7.1:2483963-2483982 MS.gene066536:CDS 60.0%
GCTGCCAAAGAAGGCTGGGA+AGG + chr7.1:2484348-2484367 MS.gene066536:CDS 60.0%
GGGAGGAAGGGGAAAACCGA+AGG + chr7.1:2483911-2483930 MS.gene066536:CDS 60.0%
GGTACCACCAAGGGAGGAAG+GGG + chr7.1:2483900-2483919 MS.gene066536:CDS 60.0%
TCTGCTGGTACCACCAAGGG+AGG + chr7.1:2483894-2483913 MS.gene066536:CDS 60.0%
! CGGTTTTCCCCTTCCTCCCT+TGG - chr7.1:2483910-2483929 None:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG + chr7.1:2484017-2484036 MS.gene066536:CDS 60.0%
CGGCGTATTTGCCGGCCTTG+AGG - chr7.1:2484004-2484023 None:intergenic 65.0%
!! ACGTGTTGGTGCCGGAGCTC+CGG + chr7.1:2484025-2484044 MS.gene066536:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 2483888 2484408 2483888 ID=MS.gene066536
chr7.1 mRNA 2483888 2484408 2483888 ID=MS.gene066536.t1;Parent=MS.gene066536
chr7.1 exon 2483888 2484085 2483888 ID=MS.gene066536.t1.exon1;Parent=MS.gene066536.t1
chr7.1 CDS 2483888 2484085 2483888 ID=cds.MS.gene066536.t1;Parent=MS.gene066536.t1
chr7.1 exon 2484187 2484408 2484187 ID=MS.gene066536.t1.exon2;Parent=MS.gene066536.t1
chr7.1 CDS 2484187 2484408 2484187 ID=cds.MS.gene066536.t1;Parent=MS.gene066536.t1
Gene Sequence

>MS.gene066536

ATGGATTCTGCTGGTACCACCAAGGGAGGAAGGGGAAAACCGAAGGCTTCCAAATCAGTTTCGAGATCTTCAAAGGCCGGACTTCAATTCCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCCGGCAAATACGCCGAACGTGTTGGTGCCGGAGCTCCGGTATACCTCTCTGCGGTCCTTGAGTATCTCGCTGCTGAGGTTCGTTTCTTTTCTTCAATTTCCCTTTCACTCTCTTTTTTGTATAATTTAGGGTTCTAGATCCAATTTGATTAAACTGTATTTTAATTTTGGGTATTTAGGTTTTGGAATTGGCTGGGAACGCTGCAAGGGACAACAAGAAGAACCGTATTGTACCAAGGCACATTCAGCTTGCTGTCAGGAACGATGAAGAACTCAGCAAGCTTTTGGGAACTGTGACCATTGCTAATGGTGGTGTTCTGCCCAATATTCATCAGACTTTGCTGCCAAAGAAGGCTGGGAAGGGAAAAGGCGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>MS.gene066536.t1

MDSAGTTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGTVTIANGGVLPNIHQTLLPKKAGKGKGEIGSASQEF