AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07616


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07616.t1 MTR_7g108320 100.000 142 0 0 1 142 1 142 2.41e-99 281
MS.gene07616.t1 MTR_7g114060 95.775 142 3 1 1 142 1 139 4.50e-93 265
MS.gene07616.t1 MTR_7g114040 95.775 142 3 1 1 142 1 139 4.50e-93 265
MS.gene07616.t1 MTR_7g093910 84.507 142 22 0 1 142 1 142 1.56e-84 244
MS.gene07616.t1 MTR_7g114050 87.786 131 5 2 12 142 11 130 3.20e-73 215
MS.gene07616.t1 MTR_2g082510 86.777 121 12 1 13 129 3 123 3.30e-69 205
MS.gene07616.t1 MTR_2g082220 85.484 124 14 1 13 132 3 126 3.45e-69 204
MS.gene07616.t1 MTR_2g096610 75.000 120 30 0 21 140 21 140 4.44e-60 182
MS.gene07616.t1 MTR_7g113160 76.068 117 28 0 12 128 16 132 1.26e-59 181
MS.gene07616.t1 MTR_4g063280 79.091 110 23 0 21 130 17 126 1.35e-58 178
MS.gene07616.t1 MTR_4g064005 81.132 106 20 0 22 127 24 129 2.03e-58 178
MS.gene07616.t1 MTR_4g063410 79.439 107 22 0 22 128 24 130 2.29e-58 178
MS.gene07616.t1 MTR_4g071150 80.374 107 21 0 22 128 24 130 2.35e-58 178
MS.gene07616.t1 MTR_2g096570 81.132 106 20 0 22 127 23 128 2.38e-58 178
MS.gene07616.t1 MTR_8g086640 81.132 106 20 0 22 127 24 129 3.13e-58 177
MS.gene07616.t1 MTR_4g064967 81.132 106 20 0 22 127 24 129 4.55e-58 177
MS.gene07616.t1 MTR_6g005150 63.158 114 33 2 22 134 26 131 3.48e-42 137
MS.gene07616.t1 MTR_1g011750 58.333 132 47 4 1 126 1 130 3.31e-40 131
MS.gene07616.t1 MTR_1g011850 58.333 132 47 4 1 126 1 130 3.31e-40 131
MS.gene07616.t1 MTR_1g114050 56.250 128 53 3 1 126 1 127 2.57e-39 129
MS.gene07616.t1 MTR_1g071040 63.462 104 36 2 24 126 28 130 4.81e-38 126
MS.gene07616.t1 MTR_4g063300 67.708 96 23 1 43 130 10 105 3.62e-36 120
MS.gene07616.t1 MTR_4g005910 88.889 45 5 0 85 129 4 48 7.42e-23 85.5
MS.gene07616.t1 MTR_7g010440 70.312 64 19 0 76 139 122 185 3.73e-22 89.4
MS.gene07616.t1 MTR_4g048490 44.860 107 30 2 22 128 26 103 1.22e-20 81.3
MS.gene07616.t1 MTR_1g069770 55.224 67 30 0 65 131 32 98 5.55e-19 77.0
MS.gene07616.t1 MTR_7g027610 40.336 119 42 3 9 126 4 94 1.20e-15 68.6
MS.gene07616.t1 MTR_0653s0030 52.830 53 25 0 53 105 43 95 4.82e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07616.t1 AT1G54690 92.254 142 11 0 1 142 1 142 3.09e-91 261
MS.gene07616.t1 AT1G08880 90.845 142 13 0 1 142 1 142 2.30e-90 259
MS.gene07616.t1 AT4G27230 86.290 124 13 1 13 132 3 126 4.41e-70 207
MS.gene07616.t1 AT4G27230 86.290 124 13 1 13 132 3 126 4.41e-70 207
MS.gene07616.t1 AT5G54640 80.303 132 20 1 1 132 1 126 3.07e-69 205
MS.gene07616.t1 AT1G51060 83.871 124 16 1 13 132 3 126 1.49e-68 203
MS.gene07616.t1 AT3G20670 84.677 124 15 1 13 132 3 126 3.99e-68 202
MS.gene07616.t1 AT5G59870 72.093 129 34 1 1 127 1 129 7.06e-61 184
MS.gene07616.t1 AT5G27670 72.656 128 33 1 2 127 3 130 2.14e-58 178
MS.gene07616.t1 AT5G02560 79.439 107 22 0 21 127 23 129 1.69e-57 176
MS.gene07616.t1 AT5G02560 64.885 131 22 1 21 127 23 153 4.71e-52 163
MS.gene07616.t1 AT2G38810 62.617 107 38 2 21 126 27 132 6.03e-39 128
MS.gene07616.t1 AT2G38810 62.617 107 38 2 21 126 27 132 6.03e-39 128
MS.gene07616.t1 AT2G38810 62.617 107 38 2 21 126 27 132 6.03e-39 128
MS.gene07616.t1 AT2G38810 62.617 107 38 2 21 126 27 132 6.03e-39 128
MS.gene07616.t1 AT1G52740 55.224 134 48 4 1 126 1 130 6.76e-39 128
MS.gene07616.t1 AT3G54560 60.952 105 39 2 23 126 29 132 2.00e-37 124
MS.gene07616.t1 AT3G54560 60.952 105 39 2 23 126 29 132 2.00e-37 124
MS.gene07616.t1 AT4G13570 44.348 115 62 2 7 120 5 118 4.12e-23 87.8
MS.gene07616.t1 AT4G13570 49.451 91 44 2 31 120 35 124 3.43e-22 85.5

Find 43 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTCGCTGCTGAGGTTT+TGG 0.254221 7.1:+5627239 MS.gene07616:CDS
TGAAGAACTCAGCAAGCTTT+TGG 0.258146 7.1:+5627341 MS.gene07616:CDS
AAGCAATGTCTGATGAATAT+TGG 0.288668 7.1:-5627397 None:intergenic
GAAACGGATTTGGAGGCTTT+TGG 0.298714 7.1:-5627096 None:intergenic
TGAAGATCTTGAAACGGATT+TGG 0.320293 7.1:-5627106 None:intergenic
TGGTTTGCCTCTTCCTCCTT+TGG 0.327874 7.1:-5627076 None:intergenic
GGAAAAGGGAAAGGTGAAAT+TGG 0.334923 7.1:+5627432 MS.gene07616:CDS
GGTTGTAGTTGTGGCACCTT+TGG 0.379726 7.1:-5627055 None:intergenic
TCAGACATTGCTTCCTAAGA+AGG 0.403914 7.1:+5627407 MS.gene07616:CDS
AGCAATGTCTGATGAATATT+GGG 0.409255 7.1:-5627396 None:intergenic
TCCTCCTTTGGTTGTAGTTG+TGG 0.413422 7.1:-5627064 None:intergenic
CTTCCTAAGAAGGTTGGAAA+AGG 0.418428 7.1:+5627417 MS.gene07616:CDS
GCGTGTTGGCGCCGGTGCTC+CGG 0.455502 7.1:+5627194 MS.gene07616:CDS
TACGCCGAGCGTGTTGGCGC+CGG 0.470776 7.1:+5627186 MS.gene07616:CDS
AGATCTTGAAACGGATTTGG+AGG 0.479759 7.1:-5627103 None:intergenic
AGCACCGGCGCCAACACGCT+CGG 0.487757 7.1:-5627190 None:intergenic
ATCGCAAGATTCCTCAAGGC+TGG 0.491107 7.1:+5627159 MS.gene07616:CDS
ACATTGCTTCCTAAGAAGGT+TGG 0.502061 7.1:+5627411 MS.gene07616:CDS
GGAAAATACGCCGAGCGTGT+TGG 0.518396 7.1:+5627180 MS.gene07616:CDS
GGTGCCGTAACCATTGCTAA+TGG 0.528391 7.1:+5627363 MS.gene07616:CDS
TTCCTAAGAAGGTTGGAAAA+GGG 0.529191 7.1:+5627418 MS.gene07616:CDS
GTTTCAAGATCTTCAAAAGC+CGG 0.540874 7.1:+5627114 MS.gene07616:CDS
TCCGACGGGGAATTGAAGAC+CGG 0.548728 7.1:-5627133 None:intergenic
AGAGAGATAGACCGGAGCAC+CGG 0.550862 7.1:-5627205 None:intergenic
AGAACAGCAGAGAGATAGAC+CGG 0.553809 7.1:-5627213 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.559881 7.1:-5627147 None:intergenic
ACCACCATTAGCAATGGTTA+CGG 0.568022 7.1:-5627367 None:intergenic
GAATCTTGCGATTCTTCCGA+CGG 0.569168 7.1:-5627148 None:intergenic
ACTACAACCAAAGGAGGAAG+AGG 0.569188 7.1:+5627069 MS.gene07616:CDS
ACAGCAAGCTGAATGTGTCT+CGG 0.587168 7.1:-5627309 None:intergenic
AAGAAGGTTGGAAAAGGGAA+AGG 0.622701 7.1:+5627423 MS.gene07616:CDS
AATCCGAAAATGAGTTCCAA+AGG 0.625540 7.1:+5627039 None:intergenic
GCCGTAACCATTGCTAATGG+TGG 0.628392 7.1:+5627366 MS.gene07616:CDS
AAGAATCGCAAGATTCCTCA+AGG 0.631895 7.1:+5627155 MS.gene07616:CDS
GGTGCCACAACTACAACCAA+AGG 0.634663 7.1:+5627060 MS.gene07616:CDS
CAAAACACCACCATTAGCAA+TGG 0.637204 7.1:-5627373 None:intergenic
TCTCGAGTATCTCGCTGCTG+AGG 0.640871 7.1:+5627233 MS.gene07616:CDS
GCCGGTCTTCAATTCCCCGT+CGG 0.642556 7.1:+5627132 MS.gene07616:CDS
GCCACAACTACAACCAAAGG+AGG 0.646292 7.1:+5627063 MS.gene07616:CDS
ATTGGCTGGTAATGCTGCGA+GGG 0.649014 7.1:+5627263 MS.gene07616:CDS
GACACATTCAGCTTGCTGTG+AGG 0.685856 7.1:+5627313 MS.gene07616:CDS
ATCTTGCGATTCTTCCGACG+GGG 0.695192 7.1:-5627146 None:intergenic
AATTGGCTGGTAATGCTGCG+AGG 0.697250 7.1:+5627262 MS.gene07616:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCAATGTCTGATGAATAT+TGG - chr7.1:5627400-5627419 None:intergenic 30.0%
AGCAATGTCTGATGAATATT+GGG - chr7.1:5627399-5627418 None:intergenic 30.0%
GTTTCAAGATCTTCAAAAGC+CGG + chr7.1:5627114-5627133 MS.gene07616:CDS 35.0%
TTCCTAAGAAGGTTGGAAAA+GGG + chr7.1:5627418-5627437 MS.gene07616:CDS 35.0%
! TGAAGATCTTGAAACGGATT+TGG - chr7.1:5627109-5627128 None:intergenic 35.0%
!! GGCTTTTGAAGATCTTGAAA+CGG - chr7.1:5627115-5627134 None:intergenic 35.0%
AAGAAGGTTGGAAAAGGGAA+AGG + chr7.1:5627423-5627442 MS.gene07616:CDS 40.0%
AAGAATCGCAAGATTCCTCA+AGG + chr7.1:5627155-5627174 MS.gene07616:CDS 40.0%
ACATTGCTTCCTAAGAAGGT+TGG + chr7.1:5627411-5627430 MS.gene07616:CDS 40.0%
ACCACCATTAGCAATGGTTA+CGG - chr7.1:5627370-5627389 None:intergenic 40.0%
CAAAACACCACCATTAGCAA+TGG - chr7.1:5627376-5627395 None:intergenic 40.0%
CTTCCTAAGAAGGTTGGAAA+AGG + chr7.1:5627417-5627436 MS.gene07616:CDS 40.0%
GGAAAAGGGAAAGGTGAAAT+TGG + chr7.1:5627432-5627451 MS.gene07616:CDS 40.0%
TCAGACATTGCTTCCTAAGA+AGG + chr7.1:5627407-5627426 MS.gene07616:CDS 40.0%
! AGATCTTGAAACGGATTTGG+AGG - chr7.1:5627106-5627125 None:intergenic 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG + chr7.1:5627342-5627361 MS.gene07616:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG + chr7.1:5627341-5627360 MS.gene07616:CDS 40.0%
! TTCCCTTTTCCAACCTTCTT+AGG - chr7.1:5627423-5627442 None:intergenic 40.0%
AATCTTGCGATTCTTCCGAC+GGG - chr7.1:5627150-5627169 None:intergenic 45.0%
ACAGCAAGCTGAATGTGTCT+CGG - chr7.1:5627312-5627331 None:intergenic 45.0%
ACTACAACCAAAGGAGGAAG+AGG + chr7.1:5627069-5627088 MS.gene07616:CDS 45.0%
AGAACAGCAGAGAGATAGAC+CGG - chr7.1:5627216-5627235 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG - chr7.1:5627151-5627170 None:intergenic 45.0%
TCCTCCTTTGGTTGTAGTTG+TGG - chr7.1:5627067-5627086 None:intergenic 45.0%
!! GAAACGGATTTGGAGGCTTT+TGG - chr7.1:5627099-5627118 None:intergenic 45.0%
ATCGCAAGATTCCTCAAGGC+TGG + chr7.1:5627159-5627178 MS.gene07616:CDS 50.0%
ATCTTGCGATTCTTCCGACG+GGG - chr7.1:5627149-5627168 None:intergenic 50.0%
GCCACAACTACAACCAAAGG+AGG + chr7.1:5627063-5627082 MS.gene07616:CDS 50.0%
GCCGTAACCATTGCTAATGG+TGG + chr7.1:5627366-5627385 MS.gene07616:CDS 50.0%
GGTTGTAGTTGTGGCACCTT+TGG - chr7.1:5627058-5627077 None:intergenic 50.0%
! AATTGGCTGGTAATGCTGCG+AGG + chr7.1:5627262-5627281 MS.gene07616:CDS 50.0%
! ATTGGCTGGTAATGCTGCGA+GGG + chr7.1:5627263-5627282 MS.gene07616:CDS 50.0%
! GGTGCCACAACTACAACCAA+AGG + chr7.1:5627060-5627079 MS.gene07616:CDS 50.0%
! GGTGCCGTAACCATTGCTAA+TGG + chr7.1:5627363-5627382 MS.gene07616:CDS 50.0%
! GTATCTCGCTGCTGAGGTTT+TGG + chr7.1:5627239-5627258 MS.gene07616:CDS 50.0%
! TGGTTTGCCTCTTCCTCCTT+TGG - chr7.1:5627079-5627098 None:intergenic 50.0%
!! GACACATTCAGCTTGCTGTG+AGG + chr7.1:5627313-5627332 MS.gene07616:CDS 50.0%
!!! CGCTGCTGAGGTTTTGGAAT+TGG + chr7.1:5627245-5627264 MS.gene07616:CDS 50.0%
!!! GCTGAGGTTTTGGAATTGGC+TGG + chr7.1:5627249-5627268 MS.gene07616:CDS 50.0%
AGAGAGATAGACCGGAGCAC+CGG - chr7.1:5627208-5627227 None:intergenic 55.0%
GGAAAATACGCCGAGCGTGT+TGG + chr7.1:5627180-5627199 MS.gene07616:CDS 55.0%
TCCGACGGGGAATTGAAGAC+CGG - chr7.1:5627136-5627155 None:intergenic 55.0%
TCTCGAGTATCTCGCTGCTG+AGG + chr7.1:5627233-5627252 MS.gene07616:CDS 55.0%
! CGGCGTATTTTCCAGCCTTG+AGG - chr7.1:5627173-5627192 None:intergenic 55.0%
GCCGGTCTTCAATTCCCCGT+CGG + chr7.1:5627132-5627151 MS.gene07616:CDS 60.0%
! TACGCCGAGCGTGTTGGCGC+CGG + chr7.1:5627186-5627205 MS.gene07616:CDS 70.0%
!! AGCACCGGCGCCAACACGCT+CGG - chr7.1:5627193-5627212 None:intergenic 70.0%
!! GCGTGTTGGCGCCGGTGCTC+CGG + chr7.1:5627194-5627213 MS.gene07616:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 5627048 5627476 5627048 ID=MS.gene07616
chr7.1 mRNA 5627048 5627476 5627048 ID=MS.gene07616.t1;Parent=MS.gene07616
chr7.1 exon 5627048 5627476 5627048 ID=MS.gene07616.t1.exon1;Parent=MS.gene07616.t1
chr7.1 CDS 5627048 5627476 5627048 ID=cds.MS.gene07616.t1;Parent=MS.gene07616.t1
Gene Sequence

>MS.gene07616

ATGAGTTCCAAAGGTGCCACAACTACAACCAAAGGAGGAAGAGGCAAACCAAAAGCCTCCAAATCCGTTTCAAGATCTTCAAAAGCCGGTCTTCAATTCCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCTGGAAAATACGCCGAGCGTGTTGGCGCCGGTGCTCCGGTCTATCTCTCTGCTGTTCTCGAGTATCTCGCTGCTGAGGTTTTGGAATTGGCTGGTAATGCTGCGAGGGACAACAAAAAGAATCGAATTGTTCCGAGACACATTCAGCTTGCTGTGAGGAATGATGAAGAACTCAGCAAGCTTTTGGGTGCCGTAACCATTGCTAATGGTGGTGTTTTGCCCAATATTCATCAGACATTGCTTCCTAAGAAGGTTGGAAAAGGGAAAGGTGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>MS.gene07616.t1

MSSKGATTTTKGGRGKPKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF