Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067091.t1 | XP_003601907.1 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 7.90E-168 | 599.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067091.t1 | Q8L936 | 49.0 | 345 | 149 | 8 | 6 | 327 | 9 | 349 | 2.2e-73 | 277.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067091.t1 | G7J365 | 94.5 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 5.7e-168 | 599.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene067091 | 0.82187 | 3.21E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene067091 | -0.80289 | 4.53E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene067091 | -0.800161 | 1.64E-48 | -1.69E-46 |
| MS.gene049285 | MS.gene067091 | -0.816483 | 5.40E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene067091 | -0.837183 | 6.04E-57 | -1.69E-46 |
| MS.gene049483 | MS.gene067091 | 0.81029 | 1.24E-50 | -1.69E-46 |
| MS.gene049634 | MS.gene067091 | 0.811773 | 5.92E-51 | -1.69E-46 |
| MS.gene04986 | MS.gene067091 | 0.839002 | 2.06E-57 | -1.69E-46 |
| MS.gene05010 | MS.gene067091 | -0.830319 | 3.14E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene067091 | -0.808958 | 2.40E-50 | -1.69E-46 |
| MS.gene050165 | MS.gene067091 | -0.827499 | 1.51E-54 | -1.69E-46 |
| MS.gene050173 | MS.gene067091 | 0.825665 | 4.14E-54 | -1.69E-46 |
| MS.gene050216 | MS.gene067091 | -0.840569 | 8.04E-58 | -1.69E-46 |
| MS.gene050333 | MS.gene067091 | -0.827393 | 1.60E-54 | -1.69E-46 |
| MS.gene050381 | MS.gene067091 | 0.813739 | 2.20E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene067091 | -0.800986 | 1.11E-48 | -1.69E-46 |
| MS.gene050504 | MS.gene067091 | -0.832149 | 1.11E-55 | -1.69E-46 |
| MS.gene050518 | MS.gene067091 | -0.817378 | 3.40E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene067091 | -0.85263 | 4.07E-61 | -1.69E-46 |
| MS.gene050675 | MS.gene067091 | -0.801381 | 9.25E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene067091 | -0.802176 | 6.35E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene067091 | -0.800832 | 1.20E-48 | -1.69E-46 |
| MS.gene050883 | MS.gene067091 | 0.803546 | 3.31E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene067091 | -0.818449 | 1.95E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene067091 | 0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene067091 | 0.802466 | 5.54E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene067091 | 0.839401 | 1.62E-57 | -1.69E-46 |
| MS.gene051366 | MS.gene067091 | 0.853174 | 2.84E-61 | -1.69E-46 |
| MS.gene051367 | MS.gene067091 | 0.843371 | 1.46E-58 | -1.69E-46 |
| MS.gene051810 | MS.gene067091 | -0.817291 | 3.56E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene067091 | 0.829673 | 4.51E-55 | -1.69E-46 |
| MS.gene053561 | MS.gene067091 | 0.874918 | 4.64E-68 | -1.69E-46 |
| MS.gene054056 | MS.gene067091 | 0.836391 | 9.62E-57 | -1.69E-46 |
| MS.gene054057 | MS.gene067091 | 0.811193 | 7.91E-51 | -1.69E-46 |
| MS.gene054616 | MS.gene067091 | 0.84625 | 2.44E-59 | -1.69E-46 |
| MS.gene054642 | MS.gene067091 | -0.822359 | 2.47E-53 | -1.69E-46 |
| MS.gene054708 | MS.gene067091 | 0.82328 | 1.51E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene067091 | -0.831443 | 1.66E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene067091 | 0.854926 | 8.89E-62 | -1.69E-46 |
| MS.gene055202 | MS.gene067091 | 0.822235 | 2.64E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene067091 | -0.828648 | 8.00E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene067091 | -0.810189 | 1.30E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene067091 | -0.800855 | 1.18E-48 | -1.69E-46 |
| MS.gene055634 | MS.gene067091 | -0.844547 | 7.07E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene067091 | -0.812869 | 3.41E-51 | -1.69E-46 |
| MS.gene055776 | MS.gene067091 | -0.850479 | 1.65E-60 | -1.69E-46 |
| MS.gene055942 | MS.gene067091 | 0.85805 | 1.07E-62 | -1.69E-46 |
| MS.gene056185 | MS.gene067091 | 0.83306 | 6.62E-56 | -1.69E-46 |
| MS.gene056504 | MS.gene067091 | -0.840592 | 7.92E-58 | -1.69E-46 |
| MS.gene056550 | MS.gene067091 | 0.819948 | 8.88E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene067091 | -0.826862 | 2.15E-54 | -1.69E-46 |
| MS.gene056761 | MS.gene067091 | 0.831001 | 2.14E-55 | -1.69E-46 |
| MS.gene057014 | MS.gene067091 | 0.810824 | 9.51E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene067091 | 0.819664 | 1.03E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene067091 | 0.814793 | 1.29E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene067091 | -0.813905 | 2.02E-51 | -1.69E-46 |
| MS.gene058493 | MS.gene067091 | 0.813975 | 1.95E-51 | -1.69E-46 |
| MS.gene058494 | MS.gene067091 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene067091 | -0.834496 | 2.90E-56 | -1.69E-46 |
| MS.gene05884 | MS.gene067091 | 0.819158 | 1.35E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene067091 | -0.849212 | 3.74E-60 | -1.69E-46 |
| MS.gene059677 | MS.gene067091 | -0.834208 | 3.42E-56 | -1.69E-46 |
| MS.gene059690 | MS.gene067091 | 0.816071 | 6.68E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene067091 | 0.811631 | 6.36E-51 | -1.69E-46 |
| MS.gene059697 | MS.gene067091 | 0.802643 | 5.09E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene067091 | -0.841468 | 4.67E-58 | -1.69E-46 |
| MS.gene060337 | MS.gene067091 | 0.837256 | 5.79E-57 | -1.69E-46 |
| MS.gene060703 | MS.gene067091 | -0.845693 | 3.46E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene067091 | -0.821779 | 3.37E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene067091 | -0.810532 | 1.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067091.t1 | MTR_3g086670 | 94.495 | 327 | 17 | 1 | 2 | 328 | 3 | 328 | 0.0 | 634 |
| MS.gene067091.t1 | MTR_8g089850 | 62.462 | 325 | 112 | 6 | 7 | 328 | 9 | 326 | 2.38e-131 | 377 |
| MS.gene067091.t1 | MTR_3g099670 | 36.237 | 287 | 172 | 4 | 2 | 279 | 5 | 289 | 1.26e-55 | 184 |
| MS.gene067091.t1 | MTR_4g115420 | 26.875 | 320 | 204 | 12 | 4 | 306 | 9 | 315 | 3.14e-17 | 82.8 |
| MS.gene067091.t1 | MTR_3g106900 | 34.014 | 147 | 92 | 3 | 4 | 147 | 6 | 150 | 2.65e-16 | 80.1 |
| MS.gene067091.t1 | MTR_2g037980 | 29.902 | 204 | 125 | 6 | 4 | 192 | 7 | 207 | 4.05e-16 | 79.3 |
| MS.gene067091.t1 | MTR_4g127440 | 28.571 | 196 | 122 | 5 | 5 | 185 | 8 | 200 | 5.62e-16 | 79.0 |
| MS.gene067091.t1 | MTR_8g008860 | 35.135 | 148 | 90 | 3 | 4 | 147 | 6 | 151 | 9.86e-15 | 75.1 |
| MS.gene067091.t1 | MTR_2g008170 | 33.108 | 148 | 93 | 3 | 4 | 147 | 6 | 151 | 3.06e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067091.t1 | AT4G40080 | 48.286 | 350 | 154 | 8 | 1 | 327 | 4 | 349 | 3.14e-100 | 300 |
| MS.gene067091.t1 | AT5G10410 | 31.875 | 320 | 182 | 9 | 7 | 301 | 8 | 316 | 1.36e-34 | 129 |
| MS.gene067091.t1 | AT5G65370 | 31.046 | 306 | 173 | 9 | 1 | 280 | 1 | 294 | 1.23e-31 | 120 |
| MS.gene067091.t1 | AT5G57200 | 30.049 | 203 | 125 | 6 | 4 | 192 | 7 | 206 | 9.80e-17 | 81.3 |
| MS.gene067091.t1 | AT4G25940 | 28.641 | 206 | 123 | 6 | 1 | 185 | 4 | 206 | 1.05e-16 | 81.3 |
| MS.gene067091.t1 | AT2G01600 | 30.667 | 225 | 137 | 8 | 1 | 210 | 4 | 224 | 8.50e-16 | 78.6 |
| MS.gene067091.t1 | AT4G02650 | 34.000 | 150 | 92 | 3 | 3 | 147 | 5 | 152 | 1.90e-14 | 74.3 |
| MS.gene067091.t1 | AT2G25430 | 31.973 | 147 | 95 | 3 | 4 | 147 | 5 | 149 | 5.23e-14 | 73.2 |
| MS.gene067091.t1 | AT1G14910 | 27.675 | 271 | 166 | 11 | 31 | 284 | 36 | 293 | 6.22e-14 | 73.2 |
| MS.gene067091.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene067091.t1 | AT5G35200 | 29.947 | 187 | 121 | 6 | 4 | 185 | 11 | 192 | 1.09e-13 | 72.0 |
| MS.gene067091.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene067091.t1 | AT4G32285 | 31.293 | 147 | 96 | 3 | 4 | 147 | 5 | 149 | 1.33e-13 | 72.0 |
| MS.gene067091.t1 | AT1G03050 | 32.667 | 150 | 94 | 3 | 3 | 147 | 5 | 152 | 6.72e-13 | 69.7 |
Find 95 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACGGCGATTAAACATTTA+AGG | 0.163434 | 3.4:+80706679 | None:intergenic |
| TTGGTTAGAGTTAAAGAATT+TGG | 0.219230 | 3.4:-80706261 | MS.gene067091:CDS |
| TGGTTAGAGTTAAAGAATTT+GGG | 0.246190 | 3.4:-80706260 | MS.gene067091:CDS |
| GTCAAGAGGTGTTTGTGTTT+TGG | 0.268343 | 3.4:+80706798 | None:intergenic |
| AAACTTGGTTGTCTTGGTTT+TGG | 0.268735 | 3.4:-80706228 | MS.gene067091:CDS |
| TGATTCTTTGTTGGCCTTGA+TGG | 0.273576 | 3.4:-80706391 | MS.gene067091:CDS |
| CAAGGTGATTGTTGAGATAA+TGG | 0.276764 | 3.4:-80706325 | MS.gene067091:CDS |
| GGAGAAGAAATTCTGGGATT+TGG | 0.296571 | 3.4:-80706124 | MS.gene067091:CDS |
| CTGTGTGCTTCTAGAACTCT+TGG | 0.303930 | 3.4:-80706486 | MS.gene067091:CDS |
| TGGTTGAGGAGAAGAAATTC+TGG | 0.306064 | 3.4:-80706131 | MS.gene067091:CDS |
| TATCTTGGGAACTCTCTTCT+TGG | 0.310691 | 3.4:-80706542 | MS.gene067091:CDS |
| AGAACTCTTGGTTTCTTCCT+TGG | 0.315271 | 3.4:-80706474 | MS.gene067091:CDS |
| CTCTTGACTCTTCTTTCCTC+TGG | 0.318672 | 3.4:-80706783 | MS.gene067091:CDS |
| CCATTAGGTTTCCTTCTTCA+AGG | 0.324011 | 3.4:-80705969 | MS.gene067091:CDS |
| TGTTCACTCATTGGAGTATT+AGG | 0.324515 | 3.4:+80706351 | None:intergenic |
| TGGGAGGATGAAGGTGTATA+GGG | 0.331362 | 3.4:-80706079 | MS.gene067091:CDS |
| ACAAAACAAGTTCAATATCT+TGG | 0.345547 | 3.4:-80706557 | MS.gene067091:CDS |
| TTCTCTTAACAGATCACTAT+TGG | 0.348060 | 3.4:+80706416 | None:intergenic |
| TATGGTGATGAATGAAGTTT+TGG | 0.357854 | 3.4:-80706280 | MS.gene067091:CDS |
| ATCTTGGGAACTCTCTTCTT+GGG | 0.358410 | 3.4:-80706541 | MS.gene067091:CDS |
| TTAGGTCTGTTGATTCCATT+AGG | 0.360896 | 3.4:-80705984 | MS.gene067091:CDS |
| ACCTTGTTCTGTTCACTCAT+TGG | 0.361918 | 3.4:+80706342 | None:intergenic |
| GGTGGAAGATGATGGGGTTA+TGG | 0.363211 | 3.4:-80706298 | MS.gene067091:CDS |
| TTGGGAGGATGAAGGTGTAT+AGG | 0.379157 | 3.4:-80706080 | MS.gene067091:CDS |
| GATTGTTGAGATAATGGATT+TGG | 0.380186 | 3.4:-80706319 | MS.gene067091:CDS |
| AAGAGAAACTGATTCTTTGT+TGG | 0.385719 | 3.4:-80706400 | MS.gene067091:CDS |
| AGTTAAAGAATTTGGGGAGA+GGG | 0.391874 | 3.4:-80706253 | MS.gene067091:CDS |
| ATGAAGGTGTATAGGGAAGA+AGG | 0.403447 | 3.4:-80706072 | MS.gene067091:CDS |
| TTGGCCTTGATGGAAGGAAT+TGG | 0.410057 | 3.4:-80706381 | MS.gene067091:CDS |
| AGGGAGAAACTTGGTTGTCT+TGG | 0.426496 | 3.4:-80706234 | MS.gene067091:CDS |
| CTCTGAAGTTTGAGAGGTTG+AGG | 0.434153 | 3.4:+80706580 | None:intergenic |
| GGTTTGATGACAGAGTTCTT+AGG | 0.440749 | 3.4:-80706002 | MS.gene067091:CDS |
| CTGCTGAGTTGTGAGTGGTT+TGG | 0.441669 | 3.4:+80706706 | None:intergenic |
| ACCACCAGTGTAAGGGTAAA+CGG | 0.442204 | 3.4:+80706611 | None:intergenic |
| TGGATTTGGTGGAAGATGAT+GGG | 0.446048 | 3.4:-80706305 | MS.gene067091:CDS |
| CAAAACAAGTTCAATATCTT+GGG | 0.446855 | 3.4:-80706556 | MS.gene067091:CDS |
| TAGTTTCTACCACCAGTGTA+AGG | 0.447417 | 3.4:+80706603 | None:intergenic |
| AACTCTCTTCTTGGGTTCGA+TGG | 0.450996 | 3.4:-80706533 | MS.gene067091:CDS |
| AAGAAACTGAAAGAGGTGAT+AGG | 0.455772 | 3.4:-80706921 | MS.gene067091:CDS |
| AACGGCGATTAAACATTTAA+GGG | 0.462073 | 3.4:+80706680 | None:intergenic |
| CCTTGAAGAAGGAAACCTAA+TGG | 0.462290 | 3.4:+80705969 | None:intergenic |
| AGAGAGAATGATGATGATGA+TGG | 0.463274 | 3.4:-80706157 | MS.gene067091:CDS |
| TTTGGGGAGAGGGAGAAACT+TGG | 0.467834 | 3.4:-80706243 | MS.gene067091:CDS |
| AGAGAATTGAAGGAAAAGGT+TGG | 0.470401 | 3.4:-80706099 | MS.gene067091:CDS |
| GATGATGATGGAGGTGGTTG+AGG | 0.470627 | 3.4:-80706145 | MS.gene067091:CDS |
| CTCTGCGGTGGAGCTTCTCA+TGG | 0.472704 | 3.4:-80706736 | MS.gene067091:CDS |
| GGATTTGGTGAGAGAATTGA+AGG | 0.473257 | 3.4:-80706109 | MS.gene067091:CDS |
| ATGGATTTGGTGGAAGATGA+TGG | 0.474899 | 3.4:-80706306 | MS.gene067091:CDS |
| GAGTTAAAGAATTTGGGGAG+AGG | 0.480788 | 3.4:-80706254 | MS.gene067091:CDS |
| TGGCCTTGATGGAAGGAATT+GGG | 0.482108 | 3.4:-80706380 | MS.gene067091:CDS |
| TGAAGGTGTATAGGGAAGAA+GGG | 0.482642 | 3.4:-80706071 | MS.gene067091:CDS |
| GGTGAGAGAATTGAAGGAAA+AGG | 0.483133 | 3.4:-80706103 | MS.gene067091:CDS |
| AAGCTCCACCGCAGAGGATG+CGG | 0.484245 | 3.4:+80706743 | None:intergenic |
| ACTCTTCTTTCCTCTGGAGA+CGG | 0.488107 | 3.4:-80706777 | MS.gene067091:CDS |
| GAGAATTGAAGGAAAAGGTT+GGG | 0.493649 | 3.4:-80706098 | MS.gene067091:CDS |
| TTTCCCAATTCCTTCCATCA+AGG | 0.501907 | 3.4:+80706377 | None:intergenic |
| GGTTGAGGAGAAGAAATTCT+GGG | 0.518899 | 3.4:-80706130 | MS.gene067091:CDS |
| ATTTACAAGAACCTTGAAGA+AGG | 0.539232 | 3.4:+80705958 | None:intergenic |
| AGAGGTGATAGGATTAATGA+AGG | 0.539540 | 3.4:-80706910 | MS.gene067091:CDS |
| TGTTGAGATAATGGATTTGG+TGG | 0.546687 | 3.4:-80706316 | MS.gene067091:CDS |
| GGTGAAACGACGTCAGAGAA+AGG | 0.547020 | 3.4:-80706453 | MS.gene067091:CDS |
| GTCATCAAACCGATCAGACT+CGG | 0.547520 | 3.4:+80706014 | None:intergenic |
| GGATTTGGTGGAAGATGATG+GGG | 0.552285 | 3.4:-80706304 | MS.gene067091:CDS |
| GGAAAAGGTTGGGAGGATGA+AGG | 0.554877 | 3.4:-80706088 | MS.gene067091:CDS |
| GAGCTGATCACGGAGGATGA+AGG | 0.556396 | 3.4:+80706635 | None:intergenic |
| AGAAGGGAGGCTTAGCAGTA+CGG | 0.556866 | 3.4:-80706055 | MS.gene067091:CDS |
| AGTTGTGAGTGGTTTGGAGA+CGG | 0.568196 | 3.4:+80706712 | None:intergenic |
| TCTTTGTTGGCCTTGATGGA+AGG | 0.570838 | 3.4:-80706387 | MS.gene067091:CDS |
| ACAGAAGAACCGAGTCTGAT+CGG | 0.577525 | 3.4:-80706023 | MS.gene067091:CDS |
| TTCAATGAAGAAACTGAAAG+AGG | 0.579832 | 3.4:-80706928 | None:intergenic |
| TCCAATGAGTGAACAGAACA+AGG | 0.581482 | 3.4:-80706343 | MS.gene067091:CDS |
| TGCCACCGCATCCTCTGCGG+TGG | 0.581913 | 3.4:-80706748 | MS.gene067091:CDS |
| TCCGTTTACCCTTACACTGG+TGG | 0.585451 | 3.4:-80706612 | MS.gene067091:CDS |
| GAGTTCTAGAAGCACACAGA+AGG | 0.587402 | 3.4:+80706490 | None:intergenic |
| CTCCACCGCAGAGGATGCGG+TGG | 0.587882 | 3.4:+80706746 | None:intergenic |
| GGCTACTGCTGAGTTGTGAG+TGG | 0.589218 | 3.4:+80706701 | None:intergenic |
| GGTTAGAGTTAAAGAATTTG+GGG | 0.590495 | 3.4:-80706259 | MS.gene067091:CDS |
| CATGTGTTGTGGCACGTAGG+AGG | 0.596737 | 3.4:+80706835 | None:intergenic |
| AATTGAAGGAAAAGGTTGGG+AGG | 0.600206 | 3.4:-80706095 | MS.gene067091:CDS |
| CTCTCCGTTTACCCTTACAC+TGG | 0.601794 | 3.4:-80706615 | MS.gene067091:CDS |
| TTCTTGGGTTCGATGGTACG+CGG | 0.608953 | 3.4:-80706526 | MS.gene067091:CDS |
| AGTTTCTACCACCAGTGTAA+GGG | 0.622840 | 3.4:+80706604 | None:intergenic |
| AGGTACCGTGTTTGATGATG+TGG | 0.633958 | 3.4:+80706655 | None:intergenic |
| GGCACGTGAACCGTCTCCAG+AGG | 0.642214 | 3.4:+80706767 | None:intergenic |
| AGGTGTATAGGGAAGAAGGG+AGG | 0.646656 | 3.4:-80706068 | MS.gene067091:CDS |
| CATGAGAAGCTCCACCGCAG+AGG | 0.658489 | 3.4:+80706737 | None:intergenic |
| CAGAGGAAAGAAGAGTCAAG+AGG | 0.661118 | 3.4:+80706784 | None:intergenic |
| GTTGAAAGAGTCATGTGTTG+TGG | 0.662998 | 3.4:+80706824 | None:intergenic |
| AGTCATGTGTTGTGGCACGT+AGG | 0.663095 | 3.4:+80706832 | None:intergenic |
| GGTAAACGGAGAGCTGATCA+CGG | 0.664071 | 3.4:+80706625 | None:intergenic |
| GTGTTTGATGATGTGGTGAA+CGG | 0.664912 | 3.4:+80706662 | None:intergenic |
| ACGTGCCACCGCATCCTCTG+CGG | 0.680106 | 3.4:-80706751 | MS.gene067091:CDS |
| TCTCTGACGTCGTTTCACCA+AGG | 0.687518 | 3.4:+80706457 | None:intergenic |
| GTTCACCACATCATCAAACA+CGG | 0.734907 | 3.4:-80706660 | MS.gene067091:CDS |
| AAACGGAGAGCTGATCACGG+AGG | 0.785713 | 3.4:+80706628 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAACAAGTTCAATATCT+TGG | - | chr3.4:80706327-80706346 | MS.gene067091:CDS | 25.0% |
| ! | CAAAACAAGTTCAATATCTT+GGG | - | chr3.4:80706328-80706347 | MS.gene067091:CDS | 25.0% |
| ! | TGGTTAGAGTTAAAGAATTT+GGG | - | chr3.4:80706624-80706643 | MS.gene067091:CDS | 25.0% |
| ! | TTGGTTAGAGTTAAAGAATT+TGG | - | chr3.4:80706623-80706642 | MS.gene067091:CDS | 25.0% |
| AACGGCGATTAAACATTTAA+GGG | + | chr3.4:80706207-80706226 | None:intergenic | 30.0% | |
| GGTTAGAGTTAAAGAATTTG+GGG | - | chr3.4:80706625-80706644 | MS.gene067091:CDS | 30.0% | |
| TTCTCTTAACAGATCACTAT+TGG | + | chr3.4:80706471-80706490 | None:intergenic | 30.0% | |
| ! | AAGAGAAACTGATTCTTTGT+TGG | - | chr3.4:80706484-80706503 | MS.gene067091:CDS | 30.0% |
| !! | GATTGTTGAGATAATGGATT+TGG | - | chr3.4:80706565-80706584 | MS.gene067091:CDS | 30.0% |
| !! | TATGGTGATGAATGAAGTTT+TGG | - | chr3.4:80706604-80706623 | MS.gene067091:CDS | 30.0% |
| AAGAAACTGAAAGAGGTGAT+AGG | - | chr3.4:80705963-80705982 | MS.gene067091:CDS | 35.0% | |
| AGAGAATTGAAGGAAAAGGT+TGG | - | chr3.4:80706785-80706804 | MS.gene067091:CDS | 35.0% | |
| AGAGGTGATAGGATTAATGA+AGG | - | chr3.4:80705974-80705993 | MS.gene067091:CDS | 35.0% | |
| AGTTAAAGAATTTGGGGAGA+GGG | - | chr3.4:80706631-80706650 | MS.gene067091:CDS | 35.0% | |
| CAAGGTGATTGTTGAGATAA+TGG | - | chr3.4:80706559-80706578 | MS.gene067091:CDS | 35.0% | |
| GAACGGCGATTAAACATTTA+AGG | + | chr3.4:80706208-80706227 | None:intergenic | 35.0% | |
| GAGAATTGAAGGAAAAGGTT+GGG | - | chr3.4:80706786-80706805 | MS.gene067091:CDS | 35.0% | |
| ! | AAACTTGGTTGTCTTGGTTT+TGG | - | chr3.4:80706656-80706675 | MS.gene067091:CDS | 35.0% |
| ! | AGAGAGAATGATGATGATGA+TGG | - | chr3.4:80706727-80706746 | MS.gene067091:CDS | 35.0% |
| ! | TGTTCACTCATTGGAGTATT+AGG | + | chr3.4:80706536-80706555 | None:intergenic | 35.0% |
| !! | TGTTGAGATAATGGATTTGG+TGG | - | chr3.4:80706568-80706587 | MS.gene067091:CDS | 35.0% |
| !! | TTAGGTCTGTTGATTCCATT+AGG | - | chr3.4:80706900-80706919 | MS.gene067091:CDS | 35.0% |
| !!! | GGTTTGTGTTTTGAAGAGAT+TGG | - | chr3.4:80706694-80706713 | MS.gene067091:CDS | 35.0% |
| !!! | TTTTGTCTCTGAAGTTTGAG+AGG | + | chr3.4:80706313-80706332 | None:intergenic | 35.0% |
| AATTGAAGGAAAAGGTTGGG+AGG | - | chr3.4:80706789-80706808 | MS.gene067091:CDS | 40.0% | |
| ACCTTGTTCTGTTCACTCAT+TGG | + | chr3.4:80706545-80706564 | None:intergenic | 40.0% | |
| AGAACTCTTGGTTTCTTCCT+TGG | - | chr3.4:80706410-80706429 | MS.gene067091:CDS | 40.0% | |
| AGTTTCTACCACCAGTGTAA+GGG | + | chr3.4:80706283-80706302 | None:intergenic | 40.0% | |
| ATCTTGGGAACTCTCTTCTT+GGG | - | chr3.4:80706343-80706362 | MS.gene067091:CDS | 40.0% | |
| CCATTAGGTTTCCTTCTTCA+AGG | - | chr3.4:80706915-80706934 | MS.gene067091:CDS | 40.0% | |
| CCTTGAAGAAGGAAACCTAA+TGG | + | chr3.4:80706918-80706937 | None:intergenic | 40.0% | |
| GAGTTAAAGAATTTGGGGAG+AGG | - | chr3.4:80706630-80706649 | MS.gene067091:CDS | 40.0% | |
| GGTGAGAGAATTGAAGGAAA+AGG | - | chr3.4:80706781-80706800 | MS.gene067091:CDS | 40.0% | |
| GGTTGAGGAGAAGAAATTCT+GGG | - | chr3.4:80706754-80706773 | MS.gene067091:CDS | 40.0% | |
| GTTCACCACATCATCAAACA+CGG | - | chr3.4:80706224-80706243 | MS.gene067091:CDS | 40.0% | |
| GTTGAAAGAGTCATGTGTTG+TGG | + | chr3.4:80706063-80706082 | None:intergenic | 40.0% | |
| TAGTTTCTACCACCAGTGTA+AGG | + | chr3.4:80706284-80706303 | None:intergenic | 40.0% | |
| TATCTTGGGAACTCTCTTCT+TGG | - | chr3.4:80706342-80706361 | MS.gene067091:CDS | 40.0% | |
| TCCAATGAGTGAACAGAACA+AGG | - | chr3.4:80706541-80706560 | MS.gene067091:CDS | 40.0% | |
| TGGTTGAGGAGAAGAAATTC+TGG | - | chr3.4:80706753-80706772 | MS.gene067091:CDS | 40.0% | |
| TTTCCCAATTCCTTCCATCA+AGG | + | chr3.4:80706510-80706529 | None:intergenic | 40.0% | |
| ! | ATGAAGGTGTATAGGGAAGA+AGG | - | chr3.4:80706812-80706831 | MS.gene067091:CDS | 40.0% |
| ! | GGTTTGATGACAGAGTTCTT+AGG | - | chr3.4:80706882-80706901 | MS.gene067091:CDS | 40.0% |
| ! | TGAAGGTGTATAGGGAAGAA+GGG | - | chr3.4:80706813-80706832 | MS.gene067091:CDS | 40.0% |
| !! | AATGATGATGATGATGGAGG+TGG | - | chr3.4:80706733-80706752 | MS.gene067091:CDS | 40.0% |
| !! | ATGGATTTGGTGGAAGATGA+TGG | - | chr3.4:80706578-80706597 | MS.gene067091:CDS | 40.0% |
| !! | GAGAATGATGATGATGATGG+AGG | - | chr3.4:80706730-80706749 | MS.gene067091:CDS | 40.0% |
| !! | GGAGAAGAAATTCTGGGATT+TGG | - | chr3.4:80706760-80706779 | MS.gene067091:CDS | 40.0% |
| !! | GGATTTGGTGAGAGAATTGA+AGG | - | chr3.4:80706775-80706794 | MS.gene067091:CDS | 40.0% |
| !! | GTGTTTGATGATGTGGTGAA+CGG | + | chr3.4:80706225-80706244 | None:intergenic | 40.0% |
| !! | TGATTCTTTGTTGGCCTTGA+TGG | - | chr3.4:80706493-80706512 | MS.gene067091:CDS | 40.0% |
| !! | TGGATTTGGTGGAAGATGAT+GGG | - | chr3.4:80706579-80706598 | MS.gene067091:CDS | 40.0% |
| !! | TTTTGGAGAAGTGGTGGAAT+TGG | - | chr3.4:80706673-80706692 | MS.gene067091:CDS | 40.0% |
| !!! | AAGAGGTGTTTGTGTTTTGG+AGG | + | chr3.4:80706086-80706105 | None:intergenic | 40.0% |
| !!! | AGAGGTGTTTGTGTTTTGGA+GGG | + | chr3.4:80706085-80706104 | None:intergenic | 40.0% |
| !!! | GTCAAGAGGTGTTTGTGTTT+TGG | + | chr3.4:80706089-80706108 | None:intergenic | 40.0% |
| !!! | TTGTCTTGGTTTTGGAGAAG+TGG | - | chr3.4:80706664-80706683 | MS.gene067091:CDS | 40.0% |
| AACTCTCTTCTTGGGTTCGA+TGG | - | chr3.4:80706351-80706370 | MS.gene067091:CDS | 45.0% | |
| ACAGAAGAACCGAGTCTGAT+CGG | - | chr3.4:80706861-80706880 | MS.gene067091:CDS | 45.0% | |
| ACCACCAGTGTAAGGGTAAA+CGG | + | chr3.4:80706276-80706295 | None:intergenic | 45.0% | |
| AGGGAGAAACTTGGTTGTCT+TGG | - | chr3.4:80706650-80706669 | MS.gene067091:CDS | 45.0% | |
| CAGAGGAAAGAAGAGTCAAG+AGG | + | chr3.4:80706103-80706122 | None:intergenic | 45.0% | |
| CTCTTGACTCTTCTTTCCTC+TGG | - | chr3.4:80706101-80706120 | MS.gene067091:CDS | 45.0% | |
| GTCATCAAACCGATCAGACT+CGG | + | chr3.4:80706873-80706892 | None:intergenic | 45.0% | |
| ! | ACTCTTCTTTCCTCTGGAGA+CGG | - | chr3.4:80706107-80706126 | MS.gene067091:CDS | 45.0% |
| ! | AGTTGTGAGTGGTTTGGAGA+CGG | + | chr3.4:80706175-80706194 | None:intergenic | 45.0% |
| ! | TGGCCTTGATGGAAGGAATT+GGG | - | chr3.4:80706504-80706523 | MS.gene067091:CDS | 45.0% |
| ! | TGGGAGGATGAAGGTGTATA+GGG | - | chr3.4:80706805-80706824 | MS.gene067091:CDS | 45.0% |
| ! | TTGGGAGGATGAAGGTGTAT+AGG | - | chr3.4:80706804-80706823 | MS.gene067091:CDS | 45.0% |
| !! | AGGTACCGTGTTTGATGATG+TGG | + | chr3.4:80706232-80706251 | None:intergenic | 45.0% |
| !! | CTCTGAAGTTTGAGAGGTTG+AGG | + | chr3.4:80706307-80706326 | None:intergenic | 45.0% |
| !! | CTGTGTGCTTCTAGAACTCT+TGG | - | chr3.4:80706398-80706417 | MS.gene067091:CDS | 45.0% |
| !! | GAGTTCTAGAAGCACACAGA+AGG | + | chr3.4:80706397-80706416 | None:intergenic | 45.0% |
| !! | GGATTTGGTGGAAGATGATG+GGG | - | chr3.4:80706580-80706599 | MS.gene067091:CDS | 45.0% |
| !! | TCTTTGTTGGCCTTGATGGA+AGG | - | chr3.4:80706497-80706516 | MS.gene067091:CDS | 45.0% |
| !! | TTGGCCTTGATGGAAGGAAT+TGG | - | chr3.4:80706503-80706522 | MS.gene067091:CDS | 45.0% |
| !!! | TCTTGGTTTTGGAGAAGTGG+TGG | - | chr3.4:80706667-80706686 | MS.gene067091:CDS | 45.0% |
| AGAAGGGAGGCTTAGCAGTA+CGG | - | chr3.4:80706829-80706848 | MS.gene067091:CDS | 50.0% | |
| AGGTGTATAGGGAAGAAGGG+AGG | - | chr3.4:80706816-80706835 | MS.gene067091:CDS | 50.0% | |
| AGTCATGTGTTGTGGCACGT+AGG | + | chr3.4:80706055-80706074 | None:intergenic | 50.0% | |
| CTCTCCGTTTACCCTTACAC+TGG | - | chr3.4:80706269-80706288 | MS.gene067091:CDS | 50.0% | |
| CTGCTGAGTTGTGAGTGGTT+TGG | + | chr3.4:80706181-80706200 | None:intergenic | 50.0% | |
| GGAAAAGGTTGGGAGGATGA+AGG | - | chr3.4:80706796-80706815 | MS.gene067091:CDS | 50.0% | |
| GGTAAACGGAGAGCTGATCA+CGG | + | chr3.4:80706262-80706281 | None:intergenic | 50.0% | |
| GGTGAAACGACGTCAGAGAA+AGG | - | chr3.4:80706431-80706450 | MS.gene067091:CDS | 50.0% | |
| TCCGTTTACCCTTACACTGG+TGG | - | chr3.4:80706272-80706291 | MS.gene067091:CDS | 50.0% | |
| TCTCTGACGTCGTTTCACCA+AGG | + | chr3.4:80706430-80706449 | None:intergenic | 50.0% | |
| TTCTTGGGTTCGATGGTACG+CGG | - | chr3.4:80706358-80706377 | MS.gene067091:CDS | 50.0% | |
| TTTGGGGAGAGGGAGAAACT+TGG | - | chr3.4:80706641-80706660 | MS.gene067091:CDS | 50.0% | |
| !! | GATGATGATGGAGGTGGTTG+AGG | - | chr3.4:80706739-80706758 | MS.gene067091:CDS | 50.0% |
| !! | GGTGGAAGATGATGGGGTTA+TGG | - | chr3.4:80706586-80706605 | MS.gene067091:CDS | 50.0% |
| AAACGGAGAGCTGATCACGG+AGG | + | chr3.4:80706259-80706278 | None:intergenic | 55.0% | |
| CATGTGTTGTGGCACGTAGG+AGG | + | chr3.4:80706052-80706071 | None:intergenic | 55.0% | |
| GAGCTGATCACGGAGGATGA+AGG | + | chr3.4:80706252-80706271 | None:intergenic | 55.0% | |
| GGCTACTGCTGAGTTGTGAG+TGG | + | chr3.4:80706186-80706205 | None:intergenic | 55.0% | |
| AAGCTCCACCGCAGAGGATG+CGG | + | chr3.4:80706144-80706163 | None:intergenic | 60.0% | |
| CATGAGAAGCTCCACCGCAG+AGG | + | chr3.4:80706150-80706169 | None:intergenic | 60.0% | |
| CTCTGCGGTGGAGCTTCTCA+TGG | - | chr3.4:80706148-80706167 | MS.gene067091:CDS | 60.0% | |
| ACGTGCCACCGCATCCTCTG+CGG | - | chr3.4:80706133-80706152 | MS.gene067091:CDS | 65.0% | |
| GGCACGTGAACCGTCTCCAG+AGG | + | chr3.4:80706120-80706139 | None:intergenic | 65.0% | |
| CTCCACCGCAGAGGATGCGG+TGG | + | chr3.4:80706141-80706160 | None:intergenic | 70.0% | |
| TGCCACCGCATCCTCTGCGG+TGG | - | chr3.4:80706136-80706155 | MS.gene067091:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 80705960 | 80706946 | 80705960 | ID=MS.gene067091 |
| chr3.4 | mRNA | 80705960 | 80706946 | 80705960 | ID=MS.gene067091.t1;Parent=MS.gene067091 |
| chr3.4 | exon | 80705960 | 80706946 | 80705960 | ID=MS.gene067091.t1.exon1;Parent=MS.gene067091.t1 |
| chr3.4 | CDS | 80705960 | 80706946 | 80705960 | ID=cds.MS.gene067091.t1;Parent=MS.gene067091.t1 |
| Gene Sequence |
| Protein sequence |
