Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070474.t1 | XP_014494321.1 | 85.8 | 810 | 113 | 1 | 1 | 808 | 1 | 810 | 0.00E+00 | 1439.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070474.t1 | Q8L6Y1 | 72.9 | 807 | 204 | 4 | 6 | 808 | 1 | 796 | 0.0e+00 | 1230.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070474.t1 | A0A1S3TKN6 | 85.8 | 810 | 113 | 1 | 1 | 808 | 1 | 810 | 0.0e+00 | 1439.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene070474 | 0.823686 | 1.21E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene070474 | 0.812272 | 4.61E-51 | -1.69E-46 |
| MS.gene049343 | MS.gene070474 | 0.810619 | 1.05E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene070474 | 0.800137 | 1.66E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene070474 | 0.811963 | 5.38E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene070474 | 0.844835 | 5.91E-59 | -1.69E-46 |
| MS.gene049825 | MS.gene070474 | 0.825704 | 4.05E-54 | -1.69E-46 |
| MS.gene049850 | MS.gene070474 | 0.829209 | 5.85E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene070474 | 0.811689 | 6.17E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene070474 | 0.809118 | 2.22E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene070474 | 0.810809 | 9.58E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene070474 | 0.814248 | 1.70E-51 | -1.69E-46 |
| MS.gene050562 | MS.gene070474 | 0.812848 | 3.45E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene070474 | 0.800948 | 1.13E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene070474 | 0.837997 | 3.73E-57 | -1.69E-46 |
| MS.gene051279 | MS.gene070474 | 0.82234 | 2.50E-53 | -1.69E-46 |
| MS.gene051332 | MS.gene070474 | 0.816841 | 4.49E-52 | -1.69E-46 |
| MS.gene051700 | MS.gene070474 | 0.821861 | 3.22E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene070474 | 0.823621 | 1.25E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene070474 | 0.821524 | 3.86E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene070474 | 0.861744 | 8.27E-64 | -1.69E-46 |
| MS.gene051957 | MS.gene070474 | 0.801371 | 9.29E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene070474 | 0.802433 | 5.62E-49 | -1.69E-46 |
| MS.gene052208 | MS.gene070474 | 0.802977 | 4.34E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene070474 | 0.802688 | 4.98E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene070474 | 0.848333 | 6.55E-60 | -1.69E-46 |
| MS.gene052584 | MS.gene070474 | 0.850555 | 1.57E-60 | -1.69E-46 |
| MS.gene052773 | MS.gene070474 | 0.811534 | 6.67E-51 | -1.69E-46 |
| MS.gene05308 | MS.gene070474 | 0.811651 | 6.29E-51 | -1.69E-46 |
| MS.gene053122 | MS.gene070474 | 0.807719 | 4.41E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene070474 | 0.896898 | 2.37E-76 | -1.69E-46 |
| MS.gene05414 | MS.gene070474 | 0.804225 | 2.39E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene070474 | 0.811437 | 7.00E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene070474 | 0.814691 | 1.35E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene070474 | -0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene070474 | 0.837929 | 3.89E-57 | -1.69E-46 |
| MS.gene05458 | MS.gene070474 | 0.80882 | 2.57E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene070474 | 0.840092 | 1.07E-57 | -1.69E-46 |
| MS.gene054775 | MS.gene070474 | 0.832081 | 1.16E-55 | -1.69E-46 |
| MS.gene054991 | MS.gene070474 | 0.832508 | 9.08E-56 | -1.69E-46 |
| MS.gene055166 | MS.gene070474 | 0.809629 | 1.72E-50 | -1.69E-46 |
| MS.gene055313 | MS.gene070474 | 0.811594 | 6.48E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene070474 | 0.801137 | 1.04E-48 | -1.69E-46 |
| MS.gene055466 | MS.gene070474 | 0.804821 | 1.80E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene070474 | 0.809651 | 1.70E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene070474 | 0.829844 | 4.10E-55 | -1.69E-46 |
| MS.gene055589 | MS.gene070474 | 0.806171 | 9.37E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene070474 | 0.849737 | 2.67E-60 | -1.69E-46 |
| MS.gene05604 | MS.gene070474 | 0.817263 | 3.61E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene070474 | 0.835832 | 1.33E-56 | -1.69E-46 |
| MS.gene056173 | MS.gene070474 | 0.816302 | 5.93E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene070474 | 0.803293 | 3.74E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene070474 | 0.810434 | 1.16E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene070474 | 0.826667 | 2.39E-54 | -1.69E-46 |
| MS.gene05689 | MS.gene070474 | 0.823552 | 1.30E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene070474 | 0.816257 | 6.07E-52 | -1.69E-46 |
| MS.gene057444 | MS.gene070474 | 0.800948 | 1.13E-48 | -1.69E-46 |
| MS.gene057598 | MS.gene070474 | 0.803094 | 4.11E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene070474 | 0.856568 | 2.95E-62 | -1.69E-46 |
| MS.gene058037 | MS.gene070474 | 0.804899 | 1.73E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene070474 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene070474 | 0.854641 | 1.08E-61 | -1.69E-46 |
| MS.gene058668 | MS.gene070474 | 0.823971 | 1.04E-53 | -1.69E-46 |
| MS.gene058829 | MS.gene070474 | 0.804835 | 1.79E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene070474 | 0.821707 | 3.50E-53 | -1.69E-46 |
| MS.gene059084 | MS.gene070474 | 0.806337 | 8.64E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene070474 | 0.803394 | 3.56E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene070474 | 0.855585 | 5.72E-62 | -1.69E-46 |
| MS.gene059725 | MS.gene070474 | 0.80006 | 1.72E-48 | -1.69E-46 |
| MS.gene05985 | MS.gene070474 | -0.803637 | 3.17E-49 | -1.69E-46 |
| MS.gene059935 | MS.gene070474 | 0.809978 | 1.45E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene070474 | 0.815929 | 7.19E-52 | -1.69E-46 |
| MS.gene060373 | MS.gene070474 | 0.822383 | 2.44E-53 | -1.69E-46 |
| MS.gene060521 | MS.gene070474 | 0.819064 | 1.41E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070474 | MS.gene034846 | PPI |
| MS.gene070474 | MS.gene057206 | PPI |
| MS.gene070474 | MS.gene056896 | PPI |
| MS.gene013534 | MS.gene070474 | PPI |
| MS.gene24706 | MS.gene070474 | PPI |
| MS.gene070474 | MS.gene007189 | PPI |
| MS.gene070474 | MS.gene06859 | PPI |
| MS.gene070474 | MS.gene050290 | PPI |
| MS.gene070474 | MS.gene050252 | PPI |
| MS.gene070474 | MS.gene81765 | PPI |
| MS.gene070474 | MS.gene06645 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070474.t1 | MTR_2g081180 | 79.379 | 805 | 161 | 2 | 6 | 808 | 1 | 802 | 0.0 | 1371 |
| MS.gene070474.t1 | MTR_2g081180 | 78.509 | 805 | 154 | 3 | 6 | 808 | 1 | 788 | 0.0 | 1345 |
| MS.gene070474.t1 | MTR_2g081180 | 77.640 | 805 | 157 | 3 | 6 | 808 | 1 | 784 | 0.0 | 1331 |
| MS.gene070474.t1 | MTR_2g081180 | 78.873 | 568 | 115 | 2 | 243 | 808 | 4 | 568 | 0.0 | 951 |
| MS.gene070474.t1 | MTR_2g081180 | 78.571 | 532 | 109 | 2 | 279 | 808 | 1 | 529 | 0.0 | 890 |
| MS.gene070474.t1 | MTR_8g464880 | 88.333 | 120 | 9 | 1 | 632 | 751 | 1 | 115 | 1.54e-64 | 211 |
| MS.gene070474.t1 | MTR_4g077475 | 64.706 | 68 | 24 | 0 | 743 | 810 | 64 | 131 | 1.32e-23 | 97.4 |
| MS.gene070474.t1 | MTR_4g077475 | 62.319 | 69 | 25 | 1 | 551 | 619 | 1 | 68 | 1.57e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070474.t1 | AT3G20630 | 72.862 | 807 | 204 | 4 | 6 | 808 | 1 | 796 | 0.0 | 1243 |
Find 181 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATCAAAATTTGTATTT+TGG | 0.104884 | 8.4:-44962933 | None:intergenic |
| TTGGCGCATGGTCTACTTTC+TGG | 0.199366 | 8.4:+44964104 | MS.gene070474:CDS |
| AACACAGGATATCAACTTAT+TGG | 0.227647 | 8.4:+44970964 | MS.gene070474:intron |
| CTATGAGTTTCTTCATATTC+AGG | 0.242690 | 8.4:-44960821 | None:intergenic |
| ATTTGTTGACAGGACAAAAT+TGG | 0.248332 | 8.4:+44964085 | MS.gene070474:intron |
| GGCGTTGTTATTCCTCCATC+TGG | 0.265403 | 8.4:+44961172 | MS.gene070474:CDS |
| TCAGACACTGCATCCATTTC+CGG | 0.278760 | 8.4:+44970213 | MS.gene070474:CDS |
| GAACCATGGCCTTCAGAAAT+AGG | 0.283309 | 8.4:-44969623 | None:intergenic |
| AATAGTGTTGGTTTCTTAGA+AGG | 0.292223 | 8.4:-44959246 | None:intergenic |
| TTCTTAGAAGGCCTATCTTC+TGG | 0.304039 | 8.4:-44959234 | None:intergenic |
| CATAAAGCAGACAAAGAAAT+TGG | 0.327783 | 8.4:+44959209 | MS.gene070474:CDS |
| TCCAAATTTAGCAAAACATT+TGG | 0.328071 | 8.4:+44962245 | MS.gene070474:CDS |
| ATTACAGCTTTGAACATATT+CGG | 0.328753 | 8.4:-44964434 | None:intergenic |
| TGTTAATCCTCCCAAGGAAA+TGG | 0.328991 | 8.4:+44971092 | MS.gene070474:CDS |
| TAGAGAGACAATTTCTTCAT+TGG | 0.337556 | 8.4:-44969378 | None:intergenic |
| CAGGCCAGTGTCACCGGAAA+TGG | 0.339385 | 8.4:-44970226 | None:intergenic |
| GATAGAATTTCATGTTCATC+TGG | 0.348308 | 8.4:+44965175 | MS.gene070474:CDS |
| GTGATAAGAAAGACAATCTT+TGG | 0.354423 | 8.4:+44961212 | MS.gene070474:CDS |
| TGTCTCTCTACTTGTTTCCA+TGG | 0.356143 | 8.4:+44969391 | MS.gene070474:CDS |
| AAAATGCGATTGGTAGGTTC+AGG | 0.362949 | 8.4:-44958686 | None:intergenic |
| GATTGGTCAACATTGGAAAT+AGG | 0.374998 | 8.4:+44963013 | MS.gene070474:CDS |
| CCAAACTTGAAACTCCTTGC+AGG | 0.390469 | 8.4:-44965145 | None:intergenic |
| TGGCGCATGGTCTACTTTCT+GGG | 0.390969 | 8.4:+44964105 | MS.gene070474:CDS |
| ATGTGTAACACCAGGTAATC+AGG | 0.392442 | 8.4:-44966856 | None:intergenic |
| TGACTGATATCTATGATATC+AGG | 0.393855 | 8.4:-44968350 | None:intergenic |
| TGCTTCGTTCCAATCTCTCT+AGG | 0.399630 | 8.4:+44958654 | MS.gene070474:CDS |
| GGAAAGTTACATACAATAGA+AGG | 0.402363 | 8.4:+44965197 | MS.gene070474:CDS |
| CTCTGCTTTGAGTTTGTGAA+AGG | 0.405467 | 8.4:-44965377 | None:intergenic |
| TGCCGAATAGATGAAAATTC+AGG | 0.414367 | 8.4:-44964470 | None:intergenic |
| ACACATGCGAAGATTTGTTA+TGG | 0.414720 | 8.4:+44966873 | MS.gene070474:CDS |
| TGGCACTATTACTGCTGATC+TGG | 0.415852 | 8.4:+44961351 | MS.gene070474:CDS |
| TTCTAAGAAACCAACACTAT+TGG | 0.417301 | 8.4:+44959248 | MS.gene070474:CDS |
| GTCTCTCTACTTGTTTCCAT+GGG | 0.420502 | 8.4:+44969392 | MS.gene070474:CDS |
| TGAGGCATCAGTCTCCAGTA+TGG | 0.422118 | 8.4:+44970179 | MS.gene070474:CDS |
| ATGTTTCGTGTAGGTCTTGA+GGG | 0.422275 | 8.4:+44960779 | MS.gene070474:intron |
| TTCTGGGAAATACTCTGTTC+CGG | 0.425141 | 8.4:+44964121 | MS.gene070474:CDS |
| TAAGGAGCAACTTGCAGCTT+GGG | 0.425340 | 8.4:+44961093 | MS.gene070474:CDS |
| ATACTCTGTTCCGGCATTCA+AGG | 0.426379 | 8.4:+44964130 | MS.gene070474:CDS |
| CCTGCAAGGAGTTTCAAGTT+TGG | 0.431104 | 8.4:+44965145 | MS.gene070474:CDS |
| CAAAATAATTTAGGTAAGGC+TGG | 0.434814 | 8.4:+44961033 | MS.gene070474:intron |
| GTTCCAATCTCTCTAGGGTT+CGG | 0.435089 | 8.4:+44958660 | MS.gene070474:CDS |
| TTGTTTGTTGCTTCATGCAA+TGG | 0.437604 | 8.4:-44965247 | None:intergenic |
| CAAATGCCTTCTCTAGACTT+TGG | 0.438288 | 8.4:-44963269 | None:intergenic |
| GTTGATCAGCTAATTACGTT+CGG | 0.445072 | 8.4:+44969677 | MS.gene070474:CDS |
| GACCTCATTTCCTGATTACC+TGG | 0.445780 | 8.4:+44966846 | MS.gene070474:CDS |
| CGAAGATTTGTTATGGAGGC+AGG | 0.447086 | 8.4:+44966880 | MS.gene070474:CDS |
| GTTAATCCTCCCAAGGAAAT+GGG | 0.449545 | 8.4:+44971093 | MS.gene070474:CDS |
| CGAGTTGAACGGGCTAATGA+TGG | 0.453581 | 8.4:+44965106 | MS.gene070474:CDS |
| AGTTTATTCTGTAGGACTGC+AGG | 0.454873 | 8.4:+44966820 | MS.gene070474:intron |
| AGAAATTGGTTCCAGAAGAT+AGG | 0.456451 | 8.4:+44959223 | MS.gene070474:CDS |
| CTCCTTACGTTCAGCACCAT+CGG | 0.457531 | 8.4:-44961077 | None:intergenic |
| GATCCGAACCCTAGAGAGAT+TGG | 0.459812 | 8.4:-44958663 | None:intergenic |
| CCTTGCAAACTTCTCTGCTC+CGG | 0.460703 | 8.4:+44965543 | MS.gene070474:CDS |
| TGTTTGTTGCTTCATGCAAT+GGG | 0.461560 | 8.4:-44965246 | None:intergenic |
| ATCAGGCAGGCCAGTGTCAC+CGG | 0.462555 | 8.4:-44970232 | None:intergenic |
| ATACAGATAACCCATTTCCT+TGG | 0.463944 | 8.4:-44971103 | None:intergenic |
| TACAGATAACCCATTTCCTT+GGG | 0.464634 | 8.4:-44971102 | None:intergenic |
| AACTTACTCCCTCCTCCATC+AGG | 0.464709 | 8.4:-44970249 | None:intergenic |
| TGGACCTGGTTATACAGGAT+TGG | 0.474300 | 8.4:+44962996 | MS.gene070474:CDS |
| TGCTTTATGCGCAAATAGAT+CGG | 0.477786 | 8.4:-44959195 | None:intergenic |
| CAAGCTTCTAAAGGCACCCT+TGG | 0.478820 | 8.4:-44965520 | None:intergenic |
| ACTGGCCTGCCTGATGGAGG+AGG | 0.483489 | 8.4:+44970240 | MS.gene070474:CDS |
| GACTGGATGCGGCATTGACT+TGG | 0.484260 | 8.4:+44969465 | MS.gene070474:CDS |
| AGGTCTACAGTTGGATCAGC+AGG | 0.486223 | 8.4:-44963300 | None:intergenic |
| AGATGAAAATTCAGGATGGC+TGG | 0.486516 | 8.4:-44964462 | None:intergenic |
| ATTAGAAACAAGAAGGAATA+CGG | 0.486941 | 8.4:+44964411 | MS.gene070474:intron |
| ACACTTACAACTGCATATTC+AGG | 0.488171 | 8.4:-44963320 | None:intergenic |
| GAGCAGTTAGGTACCTTCTC+TGG | 0.489496 | 8.4:-44960896 | None:intergenic |
| TTTGCAAGGCAAGCTTCTAA+AGG | 0.494194 | 8.4:-44965529 | None:intergenic |
| GGTGACACTGGCCTGCCTGA+TGG | 0.494563 | 8.4:+44970234 | MS.gene070474:CDS |
| ACTGATGGAATGATTCTTTG+TGG | 0.494947 | 8.4:+44961244 | MS.gene070474:CDS |
| ACATTGAGAAAGCGACAGAT+TGG | 0.495965 | 8.4:+44970142 | MS.gene070474:CDS |
| ATTGACATTCCTATTTCTGA+AGG | 0.497250 | 8.4:+44969614 | MS.gene070474:CDS |
| CTGATGGAATGATTCTTTGT+GGG | 0.499028 | 8.4:+44961245 | MS.gene070474:CDS |
| TCGGAAAGGATTACGTTGGT+TGG | 0.499687 | 8.4:+44959151 | MS.gene070474:CDS |
| TCCAAGACACTATCATCCTC+TGG | 0.503510 | 8.4:-44962222 | None:intergenic |
| ATCAAATGTTGGACTGGATG+CGG | 0.504815 | 8.4:+44969454 | MS.gene070474:CDS |
| GCTATAAATACATCAAATGT+TGG | 0.506211 | 8.4:+44969443 | MS.gene070474:CDS |
| TGCATATTCAGGTCTACAGT+TGG | 0.508855 | 8.4:-44963309 | None:intergenic |
| TATGCGCAGCAAAGGTCGCC+AGG | 0.508888 | 8.4:+44968373 | MS.gene070474:CDS |
| CACCCTCTTGCTGTAAAGCT+TGG | 0.510174 | 8.4:+44961331 | MS.gene070474:CDS |
| CAGTTTCACAAGGATGATCC+AGG | 0.510435 | 8.4:+44969491 | MS.gene070474:CDS |
| AAATACATCAAATGTTGGAC+TGG | 0.510805 | 8.4:+44969448 | MS.gene070474:CDS |
| TGGAATCGAATTCAAGAAAG+TGG | 0.512721 | 8.4:+44962952 | MS.gene070474:CDS |
| TCCAGAGGATGATAGTGTCT+TGG | 0.518327 | 8.4:+44962221 | MS.gene070474:CDS |
| ATTTGTTATGGAGGCAGGAT+GGG | 0.519295 | 8.4:+44966885 | MS.gene070474:CDS |
| TATACAGGATTGGTCAACAT+TGG | 0.520849 | 8.4:+44963006 | MS.gene070474:CDS |
| AGCCGATGGTGCTGAACGTA+AGG | 0.522162 | 8.4:+44961075 | MS.gene070474:CDS |
| GCTTCGTTCCAATCTCTCTA+GGG | 0.522341 | 8.4:+44958655 | MS.gene070474:CDS |
| TTTCAATGACAACAAGGTTG+GGG | 0.523089 | 8.4:+44971065 | MS.gene070474:CDS |
| GGTTGGAATTATGACAAGAC+TGG | 0.523865 | 8.4:+44959168 | MS.gene070474:CDS |
| GCGATGAGATTGTGCGTCCA+AGG | 0.524499 | 8.4:+44965503 | MS.gene070474:CDS |
| GATGAATTTCAGAGGTCAGA+AGG | 0.527016 | 8.4:+44959093 | MS.gene070474:intron |
| TGATGTGACAGTATCCATAC+TGG | 0.528032 | 8.4:-44970193 | None:intergenic |
| GACGATGATAACCGAAGCGT+TGG | 0.528314 | 8.4:+44958628 | None:intergenic |
| CGCAGCAAAGGTCGCCAGGA+TGG | 0.529367 | 8.4:+44968377 | MS.gene070474:CDS |
| CAACAAGATCATTCATATCA+CGG | 0.532315 | 8.4:+44963154 | MS.gene070474:CDS |
| TACTCCCTCCTCCATCAGGC+AGG | 0.532645 | 8.4:-44970245 | None:intergenic |
| ATTCCTATTTCTGAAGGCCA+TGG | 0.532648 | 8.4:+44969620 | MS.gene070474:CDS |
| CTTTGGCTAAATCTAACTGA+TGG | 0.536278 | 8.4:+44961229 | MS.gene070474:CDS |
| CAACTCGATCAAGAAAATGT+AGG | 0.537112 | 8.4:-44965090 | None:intergenic |
| GATTTGTTATGGAGGCAGGA+TGG | 0.541593 | 8.4:+44966884 | MS.gene070474:CDS |
| GACACTGGCCTGCCTGATGG+AGG | 0.543208 | 8.4:+44970237 | MS.gene070474:CDS |
| GACATACATACCTTGAATGC+CGG | 0.545190 | 8.4:-44964140 | None:intergenic |
| TTCTCTGGCAATTCGACAGA+AGG | 0.546298 | 8.4:-44960881 | None:intergenic |
| AAATGCGATTGGTAGGTTCA+GGG | 0.549022 | 8.4:-44958685 | None:intergenic |
| GCATCCATTTCCGGTGACAC+TGG | 0.549195 | 8.4:+44970222 | MS.gene070474:CDS |
| GTAAGGAGCAACTTGCAGCT+TGG | 0.549619 | 8.4:+44961092 | MS.gene070474:CDS |
| ACGAAGCAAATCCAACGCTT+CGG | 0.550712 | 8.4:-44958639 | None:intergenic |
| AGAGAAACTTGCAGAAGGAA+AGG | 0.551882 | 8.4:+44965396 | MS.gene070474:CDS |
| ATAGAATTTCATGTTCATCT+GGG | 0.555484 | 8.4:+44965176 | MS.gene070474:CDS |
| TTCGTGCTTGAAAATGCGAT+TGG | 0.555490 | 8.4:-44958696 | None:intergenic |
| TTCAAACATTAGAAACAAGA+AGG | 0.556304 | 8.4:+44964404 | MS.gene070474:intron |
| GCGTTGTTATTCCTCCATCT+GGG | 0.558615 | 8.4:+44961173 | MS.gene070474:CDS |
| ATTGTCTTTCTTATCACACT+TGG | 0.558797 | 8.4:-44961206 | None:intergenic |
| GTAGCAGTTCTTCACCATCC+TGG | 0.558986 | 8.4:-44968391 | None:intergenic |
| TAAAAGTCGTGTATATCCTC+CGG | 0.563457 | 8.4:-44965562 | None:intergenic |
| ATCAGTCATATGCGCAGCAA+AGG | 0.565733 | 8.4:+44968365 | MS.gene070474:CDS |
| TTGGCACATTTCCACCCAGA+TGG | 0.567451 | 8.4:-44961187 | None:intergenic |
| CGATGAGATTGTGCGTCCAA+GGG | 0.570277 | 8.4:+44965504 | MS.gene070474:CDS |
| TAATGATGGGAAAACTGAGC+TGG | 0.572138 | 8.4:+44965120 | MS.gene070474:CDS |
| AGATAACCCATTTCCTTGGG+AGG | 0.572510 | 8.4:-44971099 | None:intergenic |
| ACTGCTGATCTGGAAGCAGC+AGG | 0.573313 | 8.4:+44961361 | MS.gene070474:CDS |
| TTCTGTCGAATTGCCAGAGA+AGG | 0.575999 | 8.4:+44960883 | MS.gene070474:CDS |
| TCTTACAGCGGTCGTAGTTG+AGG | 0.577916 | 8.4:-44964266 | None:intergenic |
| GGCTTCTGTTAATCCTCCCA+AGG | 0.578435 | 8.4:+44971086 | MS.gene070474:CDS |
| CTGGCCTGCCTGATGGAGGA+GGG | 0.578722 | 8.4:+44970241 | MS.gene070474:CDS |
| ACAATTTCTTCATTGGCCGA+AGG | 0.578908 | 8.4:-44969371 | None:intergenic |
| GATGTTTCGTGTAGGTCTTG+AGG | 0.579104 | 8.4:+44960778 | MS.gene070474:intron |
| CACCAGGTAATCAGGAAATG+AGG | 0.581945 | 8.4:-44966848 | None:intergenic |
| GTGAAGAACTGCTACCTGAT+GGG | 0.584775 | 8.4:+44968399 | MS.gene070474:CDS |
| TTATAGCAGCTTTCTGACAA+CGG | 0.586766 | 8.4:-44969427 | None:intergenic |
| GTTTCGTGTAGGTCTTGAGG+GGG | 0.588680 | 8.4:+44960781 | MS.gene070474:intron |
| ATACTGGAGACTGATGCCTC+AGG | 0.590591 | 8.4:-44970177 | None:intergenic |
| GTGCCAAGCTTTACAGCAAG+AGG | 0.591223 | 8.4:-44961334 | None:intergenic |
| GAGTTGAACGGGCTAATGAT+GGG | 0.592153 | 8.4:+44965107 | MS.gene070474:CDS |
| GGTGAAGAACTGCTACCTGA+TGG | 0.592381 | 8.4:+44968398 | MS.gene070474:CDS |
| TGAAGAACTGCTACCTGATG+GGG | 0.593296 | 8.4:+44968400 | MS.gene070474:CDS |
| TGCTTGAAAATGCGATTGGT+AGG | 0.595745 | 8.4:-44958692 | None:intergenic |
| AATTTCAGAGGTCAGAAGGT+GGG | 0.598431 | 8.4:+44959097 | MS.gene070474:intron |
| GTTATCTGTATTTCTTCGAG+AGG | 0.598528 | 8.4:+44971115 | MS.gene070474:CDS |
| GACACTATCATCCTCTGGAT+AGG | 0.601245 | 8.4:-44962217 | None:intergenic |
| CTGCTGTAAACTCACTGCAT+AGG | 0.601837 | 8.4:-44961140 | None:intergenic |
| TTGGTCAAGCTCTCTTTCAG+CGG | 0.603928 | 8.4:-44962914 | None:intergenic |
| TTGTTATTCCTCCATCTGGG+TGG | 0.606247 | 8.4:+44961176 | MS.gene070474:CDS |
| AGTTTACAGCAGATTGACAA+TGG | 0.608628 | 8.4:+44961151 | MS.gene070474:CDS |
| ATACCTACAGCCAATAGTGT+TGG | 0.609740 | 8.4:-44959258 | None:intergenic |
| GCACATTTCCACCCAGATGG+AGG | 0.611161 | 8.4:-44961184 | None:intergenic |
| GCTTTCGGAAAGGATTACGT+TGG | 0.616166 | 8.4:+44959147 | MS.gene070474:CDS |
| TTGCGTTTCTTACGACACTC+CGG | 0.616701 | 8.4:+44958721 | MS.gene070474:CDS |
| TTGCATGAAGCAACAAACAA+AGG | 0.616933 | 8.4:+44965250 | MS.gene070474:CDS |
| AAACTGAGCTGGATCCTGCA+AGG | 0.617385 | 8.4:+44965131 | MS.gene070474:CDS |
| GACTTGGTTACAGTTTCACA+AGG | 0.617561 | 8.4:+44969481 | MS.gene070474:CDS |
| GAATTTCAGAGGTCAGAAGG+TGG | 0.618238 | 8.4:+44959096 | MS.gene070474:intron |
| ACGGAGATGATCAAAGCCCA+TGG | 0.619505 | 8.4:-44969408 | None:intergenic |
| GACAGGACAAAATTGGCGCA+TGG | 0.621055 | 8.4:+44964092 | MS.gene070474:intron |
| CTTGAACCAAAGTCTAGAGA+AGG | 0.624274 | 8.4:+44963263 | MS.gene070474:CDS |
| GATGCAATCTTACTAGCCGA+TGG | 0.625399 | 8.4:+44961061 | MS.gene070474:CDS |
| ATTGGAGTTGTTAGCCACAG+TGG | 0.628087 | 8.4:+44970982 | MS.gene070474:CDS |
| TTGTCAAAGCTTCAGAACCA+TGG | 0.631887 | 8.4:-44969637 | None:intergenic |
| AAACCAACACTATTGGCTGT+AGG | 0.633486 | 8.4:+44959255 | MS.gene070474:CDS |
| TTGACCAATCCTGTATAACC+AGG | 0.634222 | 8.4:-44963000 | None:intergenic |
| TCTCTGGCAATTCGACAGAA+GGG | 0.635011 | 8.4:-44960880 | None:intergenic |
| CCGGAGCAGAGAAGTTTGCA+AGG | 0.637462 | 8.4:-44965543 | None:intergenic |
| AAAGCAGAGAAACTTGCAGA+AGG | 0.639104 | 8.4:+44965391 | MS.gene070474:CDS |
| TGCAAACTTCTCTGCTCCGG+AGG | 0.639169 | 8.4:+44965546 | MS.gene070474:CDS |
| GGAGCAACTTGCAGCTTGGG+CGG | 0.640605 | 8.4:+44961096 | MS.gene070474:CDS |
| ATGGAATGATTCTTTGTGGG+AGG | 0.642009 | 8.4:+44961248 | MS.gene070474:CDS |
| TGTTTCGTGTAGGTCTTGAG+GGG | 0.645173 | 8.4:+44960780 | MS.gene070474:intron |
| CATGCGAAGATTTGTTATGG+AGG | 0.646797 | 8.4:+44966876 | MS.gene070474:CDS |
| TTGGATATTTAGCAATCCTG+AGG | 0.649412 | 8.4:+44970161 | MS.gene070474:CDS |
| AATGAAAGAGACACATACTC+AGG | 0.656070 | 8.4:-44960857 | None:intergenic |
| TGCCAAGCTTTACAGCAAGA+GGG | 0.664548 | 8.4:-44961333 | None:intergenic |
| TTTCGTGTAGGTCTTGAGGG+GGG | 0.668238 | 8.4:+44960782 | MS.gene070474:intron |
| ATCTTCGCATGTGTAACACC+AGG | 0.671341 | 8.4:-44966864 | None:intergenic |
| AGTGGAACCTCTACGCTGTG+TGG | 0.697486 | 8.4:+44971000 | MS.gene070474:CDS |
| GAATAGATGAAAATTCAGGA+TGG | 0.699038 | 8.4:-44964466 | None:intergenic |
| GAAGAACTGCTACCTGATGG+GGG | 0.708896 | 8.4:+44968401 | MS.gene070474:CDS |
| AGACGTTTACTCCTATCCAG+AGG | 0.709737 | 8.4:+44962206 | MS.gene070474:intron |
| GTAGTGACCACACAGCGTAG+AGG | 0.713372 | 8.4:-44971007 | None:intergenic |
| ACAGCGTAGAGGTTCCACTG+TGG | 0.816807 | 8.4:-44970996 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 44958632 | 44971149 | 44958632 | ID=MS.gene070474 |
| chr8.4 | mRNA | 44958632 | 44971149 | 44958632 | ID=MS.gene070474.t1;Parent=MS.gene070474 |
| chr8.4 | exon | 44958632 | 44958742 | 44958632 | ID=MS.gene070474.t1.exon1;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44958632 | 44958742 | 44958632 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44959105 | 44959276 | 44959105 | ID=MS.gene070474.t1.exon2;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44959105 | 44959276 | 44959105 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44960792 | 44960904 | 44960792 | ID=MS.gene070474.t1.exon3;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44960792 | 44960904 | 44960792 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44961046 | 44961382 | 44961046 | ID=MS.gene070474.t1.exon4;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44961046 | 44961382 | 44961046 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44962208 | 44962302 | 44962208 | ID=MS.gene070474.t1.exon5;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44962208 | 44962302 | 44962208 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44962901 | 44963034 | 44962901 | ID=MS.gene070474.t1.exon6;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44962901 | 44963034 | 44962901 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44963119 | 44963175 | 44963119 | ID=MS.gene070474.t1.exon7;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44963119 | 44963175 | 44963119 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44963257 | 44963334 | 44963257 | ID=MS.gene070474.t1.exon8;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44963257 | 44963334 | 44963257 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44964097 | 44964151 | 44964097 | ID=MS.gene070474.t1.exon9;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44964097 | 44964151 | 44964097 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44964241 | 44964282 | 44964241 | ID=MS.gene070474.t1.exon10;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44964241 | 44964282 | 44964241 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44964416 | 44964496 | 44964416 | ID=MS.gene070474.t1.exon11;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44964416 | 44964496 | 44964416 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44965073 | 44965271 | 44965073 | ID=MS.gene070474.t1.exon12;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44965073 | 44965271 | 44965073 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44965365 | 44965422 | 44965365 | ID=MS.gene070474.t1.exon13;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44965365 | 44965422 | 44965365 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44965498 | 44965620 | 44965498 | ID=MS.gene070474.t1.exon14;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44965498 | 44965620 | 44965498 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44966834 | 44966922 | 44966834 | ID=MS.gene070474.t1.exon15;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44966834 | 44966922 | 44966834 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44968333 | 44968422 | 44968333 | ID=MS.gene070474.t1.exon16;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44968333 | 44968422 | 44968333 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44969351 | 44969512 | 44969351 | ID=MS.gene070474.t1.exon17;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44969351 | 44969512 | 44969351 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44969612 | 44969739 | 44969612 | ID=MS.gene070474.t1.exon18;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44969612 | 44969739 | 44969612 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44970135 | 44970264 | 44970135 | ID=MS.gene070474.t1.exon19;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44970135 | 44970264 | 44970135 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| chr8.4 | exon | 44970971 | 44971149 | 44970971 | ID=MS.gene070474.t1.exon20;Parent=MS.gene070474.t1 |
| chr8.4 | CDS | 44970971 | 44971149 | 44970971 | ID=cds.MS.gene070474.t1;Parent=MS.gene070474.t1 |
| Gene Sequence |
| Protein sequence |
