Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070666.t1 | XP_013463512.1 | 95 | 120 | 5 | 1 | 1 | 119 | 1 | 120 | 2.40E-42 | 181.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070666.t1 | A0A072V6T7 | 95.0 | 120 | 5 | 1 | 1 | 119 | 1 | 120 | 1.8e-42 | 181.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene070666 | 0.829592 | 4.72E-55 | -1.69E-46 |
| MS.gene049491 | MS.gene070666 | 0.854971 | 8.63E-62 | -1.69E-46 |
| MS.gene049614 | MS.gene070666 | 0.835097 | 2.05E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene070666 | 0.825486 | 4.57E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene070666 | 0.817929 | 2.55E-52 | -1.69E-46 |
| MS.gene049760 | MS.gene070666 | 0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene050076 | MS.gene070666 | 0.86076 | 1.65E-63 | -1.69E-46 |
| MS.gene050301 | MS.gene070666 | 0.800149 | 1.65E-48 | -1.69E-46 |
| MS.gene050432 | MS.gene070666 | 0.865873 | 4.30E-65 | -1.69E-46 |
| MS.gene05053 | MS.gene070666 | 0.821555 | 3.79E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene070666 | 0.903057 | 5.14E-79 | -1.69E-46 |
| MS.gene050770 | MS.gene070666 | 0.903533 | 3.14E-79 | -1.69E-46 |
| MS.gene05101 | MS.gene070666 | 0.843629 | 1.25E-58 | -1.69E-46 |
| MS.gene051228 | MS.gene070666 | 0.850604 | 1.52E-60 | -1.69E-46 |
| MS.gene051471 | MS.gene070666 | 0.808848 | 2.53E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene070666 | 0.804858 | 1.77E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene070666 | 0.86585 | 4.38E-65 | -1.69E-46 |
| MS.gene052290 | MS.gene070666 | 0.869626 | 2.69E-66 | -1.69E-46 |
| MS.gene052293 | MS.gene070666 | 0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene052310 | MS.gene070666 | 0.827291 | 1.70E-54 | -1.69E-46 |
| MS.gene052326 | MS.gene070666 | 0.841719 | 4.01E-58 | -1.69E-46 |
| MS.gene052379 | MS.gene070666 | 0.838361 | 3.01E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene070666 | 0.804952 | 1.69E-49 | -1.69E-46 |
| MS.gene053009 | MS.gene070666 | 0.849264 | 3.62E-60 | -1.69E-46 |
| MS.gene053103 | MS.gene070666 | 0.836793 | 7.60E-57 | -1.69E-46 |
| MS.gene053104 | MS.gene070666 | 0.843432 | 1.41E-58 | -1.69E-46 |
| MS.gene053209 | MS.gene070666 | 0.824218 | 9.09E-54 | -1.69E-46 |
| MS.gene053322 | MS.gene070666 | 0.862682 | 4.26E-64 | -1.69E-46 |
| MS.gene053384 | MS.gene070666 | 0.811827 | 5.76E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene070666 | 0.836309 | 1.01E-56 | -1.69E-46 |
| MS.gene054376 | MS.gene070666 | 0.903119 | 4.82E-79 | -1.69E-46 |
| MS.gene054543 | MS.gene070666 | 0.819296 | 1.25E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene070666 | 0.807386 | 5.19E-50 | -1.69E-46 |
| MS.gene054802 | MS.gene070666 | 0.837208 | 5.95E-57 | -1.69E-46 |
| MS.gene054803 | MS.gene070666 | 0.840484 | 8.46E-58 | -1.69E-46 |
| MS.gene054804 | MS.gene070666 | 0.84072 | 7.34E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene070666 | 0.804959 | 1.68E-49 | -1.69E-46 |
| MS.gene055254 | MS.gene070666 | 0.86523 | 6.86E-65 | -1.69E-46 |
| MS.gene055277 | MS.gene070666 | 0.854745 | 1.00E-61 | -1.69E-46 |
| MS.gene055397 | MS.gene070666 | 0.867666 | 1.16E-65 | -1.69E-46 |
| MS.gene055412 | MS.gene070666 | 0.803375 | 3.59E-49 | -1.69E-46 |
| MS.gene055497 | MS.gene070666 | -0.804667 | 1.94E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene070666 | 0.865925 | 4.15E-65 | -1.69E-46 |
| MS.gene055636 | MS.gene070666 | 0.826933 | 2.07E-54 | -1.69E-46 |
| MS.gene055647 | MS.gene070666 | 0.854275 | 1.37E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene070666 | 0.807727 | 4.39E-50 | -1.69E-46 |
| MS.gene055896 | MS.gene070666 | 0.814486 | 1.50E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene070666 | 0.871522 | 6.41E-67 | -1.69E-46 |
| MS.gene056374 | MS.gene070666 | 0.825059 | 5.76E-54 | -1.69E-46 |
| MS.gene056410 | MS.gene070666 | 0.822785 | 1.97E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene070666 | 0.847594 | 1.05E-59 | -1.69E-46 |
| MS.gene057011 | MS.gene070666 | 0.825109 | 5.61E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene070666 | 0.804883 | 1.74E-49 | -1.69E-46 |
| MS.gene057123 | MS.gene070666 | 0.82418 | 9.28E-54 | -1.69E-46 |
| MS.gene057129 | MS.gene070666 | 0.869488 | 2.98E-66 | -1.69E-46 |
| MS.gene057146 | MS.gene070666 | 0.818687 | 1.72E-52 | -1.69E-46 |
| MS.gene057295 | MS.gene070666 | 0.815283 | 1.00E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene070666 | 0.817716 | 2.85E-52 | -1.69E-46 |
| MS.gene05735 | MS.gene070666 | 0.817998 | 2.47E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene070666 | 0.825981 | 3.48E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene070666 | 0.824262 | 8.88E-54 | -1.69E-46 |
| MS.gene058459 | MS.gene070666 | 0.808469 | 3.05E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene070666 | 0.84265 | 2.27E-58 | -1.69E-46 |
| MS.gene058903 | MS.gene070666 | 0.82248 | 2.32E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene070666 | 0.81674 | 4.73E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene070666 | 0.817483 | 3.22E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene070666 | 0.834156 | 3.53E-56 | -1.69E-46 |
| MS.gene059597 | MS.gene070666 | 0.816341 | 5.81E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene070666 | 0.818149 | 2.28E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene070666 | 0.827855 | 1.24E-54 | -1.69E-46 |
| MS.gene059692 | MS.gene070666 | 0.832121 | 1.13E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene070666 | 0.830005 | 3.75E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene070666 | 0.859835 | 3.14E-63 | -1.69E-46 |
| MS.gene059894 | MS.gene070666 | 0.81914 | 1.36E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene070666 | 0.865422 | 5.98E-65 | -1.69E-46 |
| MS.gene059974 | MS.gene070666 | 0.829829 | 4.14E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene070666 | 0.830006 | 3.74E-55 | -1.69E-46 |
| MS.gene060356 | MS.gene070666 | 0.800033 | 1.74E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene070666 | 0.809078 | 2.26E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene070666 | 0.863188 | 2.97E-64 | -1.69E-46 |
| MS.gene060818 | MS.gene070666 | 0.866848 | 2.11E-65 | -1.69E-46 |
| MS.gene061041 | MS.gene070666 | 0.826273 | 2.97E-54 | -1.69E-46 |
| MS.gene061051 | MS.gene070666 | -0.839376 | 1.64E-57 | -1.69E-46 |
| MS.gene061134 | MS.gene070666 | 0.814664 | 1.37E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene070666 | 0.868486 | 6.30E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070666.t1 | MTR_2g041260 | 95.000 | 120 | 5 | 1 | 1 | 119 | 1 | 120 | 7.67e-60 | 180 |
| MS.gene070666.t1 | MTR_4g125600 | 64.167 | 120 | 37 | 3 | 1 | 118 | 1 | 116 | 6.30e-42 | 134 |
| MS.gene070666.t1 | MTR_1g015290 | 56.000 | 125 | 47 | 4 | 1 | 118 | 1 | 124 | 2.49e-35 | 118 |
| MS.gene070666.t1 | MTR_3g104950 | 54.918 | 122 | 48 | 4 | 1 | 118 | 1 | 119 | 5.09e-35 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070666.t1 | AT2G06520 | 55.738 | 122 | 43 | 3 | 1 | 118 | 1 | 115 | 1.12e-29 | 103 |
Find 40 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGCTTGACGGGATCAAAAT+TGG | 0.246839 | 2.4:+48926887 | None:intergenic |
| CGATACAACTTTCTCCTTCA+TGG | 0.258090 | 2.4:+48927058 | None:intergenic |
| GTTGTGGTTGTAGTTATTAT+TGG | 0.278706 | 2.4:-48926927 | MS.gene070666:CDS |
| GCAATGGAAGATTTCAAGTT+AGG | 0.304382 | 2.4:-48927085 | MS.gene070666:CDS |
| AGCTATTCACCACCCTCTTC+TGG | 0.329240 | 2.4:+48927168 | None:intergenic |
| TTCTTGCTCAGCATTGCAGC+TGG | 0.329837 | 2.4:-48926954 | MS.gene070666:CDS |
| TCTTCGGTCTTCAAAGGCTT+CGG | 0.384186 | 2.4:-48927120 | MS.gene070666:CDS |
| TCTTCAATCCACTTCCTCTT+CGG | 0.411814 | 2.4:-48927136 | MS.gene070666:CDS |
| CAATGGAAGATTTCAAGTTA+GGG | 0.418208 | 2.4:-48927084 | MS.gene070666:CDS |
| ATTTATCAGCGTCGCTTGAC+GGG | 0.428873 | 2.4:+48926876 | None:intergenic |
| TTCTGGCCGGTGTAAGTTAG+TGG | 0.442835 | 2.4:+48927185 | None:intergenic |
| AATTTATCAGCGTCGCTTGA+CGG | 0.443913 | 2.4:+48926875 | None:intergenic |
| CTTGCTCAGCATTGCAGCTG+GGG | 0.446840 | 2.4:-48926952 | MS.gene070666:CDS |
| TTTGAAGACCGAAGAGGAAG+TGG | 0.478145 | 2.4:+48927128 | None:intergenic |
| ATGAAGGAGAAAGTTGTATC+GGG | 0.488290 | 2.4:-48927056 | MS.gene070666:CDS |
| TCTTGCTCAGCATTGCAGCT+GGG | 0.491257 | 2.4:-48926953 | MS.gene070666:CDS |
| ACTTCCTCTTCGGTCTTCAA+AGG | 0.493262 | 2.4:-48927126 | MS.gene070666:CDS |
| CTCCACTTCTGCAGTTTCAG+TGG | 0.496724 | 2.4:-48927213 | MS.gene070666:CDS |
| TTCAATGCTGGTTCCTGATG+TGG | 0.502456 | 2.4:-48927009 | MS.gene070666:CDS |
| AGCATTGACAGCTTCAATGC+TGG | 0.508169 | 2.4:-48927021 | MS.gene070666:CDS |
| TGCCACTGAAACTGCAGAAG+TGG | 0.518999 | 2.4:+48927211 | None:intergenic |
| CATGAAGGAGAAAGTTGTAT+CGG | 0.528319 | 2.4:-48927057 | MS.gene070666:CDS |
| TCAAGTTAGGGCTTCCATGA+AGG | 0.532236 | 2.4:-48927072 | MS.gene070666:CDS |
| TCGGAATCTTTGAAACGCAA+TGG | 0.543756 | 2.4:-48927101 | MS.gene070666:CDS |
| TTGCTCAGCATTGCAGCTGG+GGG | 0.544179 | 2.4:-48926951 | MS.gene070666:CDS |
| AGCAAGAAGTTCTTGAGAGA+AGG | 0.544406 | 2.4:+48926969 | None:intergenic |
| ACGGCCGCTTCAGCCACATC+AGG | 0.547544 | 2.4:+48926996 | None:intergenic |
| ATTCACCACCCTCTTCTGGC+CGG | 0.567755 | 2.4:+48927172 | None:intergenic |
| TAACTTACACCGGCCAGAAG+AGG | 0.570657 | 2.4:-48927181 | MS.gene070666:CDS |
| CACTGAAACTGCAGAAGTGG+AGG | 0.579775 | 2.4:+48927214 | None:intergenic |
| AAGTGGATTGAAGAATGCCT+TGG | 0.581435 | 2.4:+48927145 | None:intergenic |
| GTTCTTGAGAGAAGGTGAAA+CGG | 0.587992 | 2.4:+48926977 | None:intergenic |
| AACTTACACCGGCCAGAAGA+GGG | 0.588837 | 2.4:-48927180 | MS.gene070666:CDS |
| GGTTCCTGATGTGGCTGAAG+CGG | 0.602673 | 2.4:-48927000 | MS.gene070666:CDS |
| GAAGCCTTTGAAGACCGAAG+AGG | 0.644109 | 2.4:+48927122 | None:intergenic |
| GAGGGTGGTGAATAGCTCCA+AGG | 0.662303 | 2.4:-48927162 | MS.gene070666:CDS |
| GCAATGCCACTAACTTACAC+CGG | 0.669538 | 2.4:-48927191 | MS.gene070666:CDS |
| GGTTGTAGTTATTATTGGTG+CGG | 0.673405 | 2.4:-48926922 | MS.gene070666:CDS |
| TGAAGGAGAAAGTTGTATCG+GGG | 0.689252 | 2.4:-48927055 | MS.gene070666:CDS |
| TTACACCGGCCAGAAGAGGG+TGG | 0.718271 | 2.4:-48927177 | MS.gene070666:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTGTGGTTGTAGTTATTAT+TGG | - | chr2.4:48927172-48927191 | MS.gene070666:CDS | 30.0% | |
| !! | CAATGGAAGATTTCAAGTTA+GGG | - | chr2.4:48927015-48927034 | MS.gene070666:CDS | 30.0% |
| ATGAAGGAGAAAGTTGTATC+GGG | - | chr2.4:48927043-48927062 | MS.gene070666:CDS | 35.0% | |
| CATGAAGGAGAAAGTTGTAT+CGG | - | chr2.4:48927042-48927061 | MS.gene070666:CDS | 35.0% | |
| GGTTGTAGTTATTATTGGTG+CGG | - | chr2.4:48927177-48927196 | MS.gene070666:CDS | 35.0% | |
| !! | GCAATGGAAGATTTCAAGTT+AGG | - | chr2.4:48927014-48927033 | MS.gene070666:CDS | 35.0% |
| AGCAAGAAGTTCTTGAGAGA+AGG | + | chr2.4:48927133-48927152 | None:intergenic | 40.0% | |
| CGATACAACTTTCTCCTTCA+TGG | + | chr2.4:48927044-48927063 | None:intergenic | 40.0% | |
| GTTCTTGAGAGAAGGTGAAA+CGG | + | chr2.4:48927125-48927144 | None:intergenic | 40.0% | |
| TCTTCAATCCACTTCCTCTT+CGG | - | chr2.4:48926963-48926982 | MS.gene070666:CDS | 40.0% | |
| TGAAGGAGAAAGTTGTATCG+GGG | - | chr2.4:48927044-48927063 | MS.gene070666:CDS | 40.0% | |
| !! | AAGTGGATTGAAGAATGCCT+TGG | + | chr2.4:48926957-48926976 | None:intergenic | 40.0% |
| !! | TCGGAATCTTTGAAACGCAA+TGG | - | chr2.4:48926998-48927017 | MS.gene070666:CDS | 40.0% |
| ACTTCCTCTTCGGTCTTCAA+AGG | - | chr2.4:48926973-48926992 | MS.gene070666:CDS | 45.0% | |
| GCAATGCCACTAACTTACAC+CGG | - | chr2.4:48926908-48926927 | MS.gene070666:CDS | 45.0% | |
| TCAAGTTAGGGCTTCCATGA+AGG | - | chr2.4:48927027-48927046 | MS.gene070666:CDS | 45.0% | |
| TCGCTTGACGGGATCAAAAT+TGG | + | chr2.4:48927215-48927234 | None:intergenic | 45.0% | |
| TCTTCGGTCTTCAAAGGCTT+CGG | - | chr2.4:48926979-48926998 | MS.gene070666:CDS | 45.0% | |
| TTTGAAGACCGAAGAGGAAG+TGG | + | chr2.4:48926974-48926993 | None:intergenic | 45.0% | |
| !! | AGCATTGACAGCTTCAATGC+TGG | - | chr2.4:48927078-48927097 | MS.gene070666:CDS | 45.0% |
| !! | TTCAATGCTGGTTCCTGATG+TGG | - | chr2.4:48927090-48927109 | MS.gene070666:CDS | 45.0% |
| AACTTACACCGGCCAGAAGA+GGG | - | chr2.4:48926919-48926938 | MS.gene070666:CDS | 50.0% | |
| AGCTATTCACCACCCTCTTC+TGG | + | chr2.4:48926934-48926953 | None:intergenic | 50.0% | |
| CACTGAAACTGCAGAAGTGG+AGG | + | chr2.4:48926888-48926907 | None:intergenic | 50.0% | |
| CTCCACTTCTGCAGTTTCAG+TGG | - | chr2.4:48926886-48926905 | MS.gene070666:CDS | 50.0% | |
| TAACTTACACCGGCCAGAAG+AGG | - | chr2.4:48926918-48926937 | MS.gene070666:CDS | 50.0% | |
| TCTTGCTCAGCATTGCAGCT+GGG | - | chr2.4:48927146-48927165 | MS.gene070666:CDS | 50.0% | |
| TGCCACTGAAACTGCAGAAG+TGG | + | chr2.4:48926891-48926910 | None:intergenic | 50.0% | |
| TTCTGGCCGGTGTAAGTTAG+TGG | + | chr2.4:48926917-48926936 | None:intergenic | 50.0% | |
| TTCTTGCTCAGCATTGCAGC+TGG | - | chr2.4:48927145-48927164 | MS.gene070666:CDS | 50.0% | |
| ! | GAAGCCTTTGAAGACCGAAG+AGG | + | chr2.4:48926980-48926999 | None:intergenic | 50.0% |
| ATTCACCACCCTCTTCTGGC+CGG | + | chr2.4:48926930-48926949 | None:intergenic | 55.0% | |
| CTTGCTCAGCATTGCAGCTG+GGG | - | chr2.4:48927147-48927166 | MS.gene070666:CDS | 55.0% | |
| GAGGGTGGTGAATAGCTCCA+AGG | - | chr2.4:48926937-48926956 | MS.gene070666:CDS | 55.0% | |
| GGTTCCTGATGTGGCTGAAG+CGG | - | chr2.4:48927099-48927118 | MS.gene070666:CDS | 55.0% | |
| TTGCTCAGCATTGCAGCTGG+GGG | - | chr2.4:48927148-48927167 | MS.gene070666:CDS | 55.0% | |
| CATTGCAGCTGGGGGAGTTG+TGG | - | chr2.4:48927156-48927175 | MS.gene070666:CDS | 60.0% | |
| TTACACCGGCCAGAAGAGGG+TGG | - | chr2.4:48926922-48926941 | MS.gene070666:CDS | 60.0% | |
| ACGGCCGCTTCAGCCACATC+AGG | + | chr2.4:48927106-48927125 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 48926881 | 48927240 | 48926881 | ID=MS.gene070666 |
| chr2.4 | mRNA | 48926881 | 48927240 | 48926881 | ID=MS.gene070666.t1;Parent=MS.gene070666 |
| chr2.4 | exon | 48926881 | 48927240 | 48926881 | ID=MS.gene070666.t1.exon1;Parent=MS.gene070666.t1 |
| chr2.4 | CDS | 48926881 | 48927240 | 48926881 | ID=cds.MS.gene070666.t1;Parent=MS.gene070666.t1 |
| Gene Sequence |
| Protein sequence |
