AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054543


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054543.t1 MTR_4g007620 50.549 182 63 4 1 181 13 168 3.10e-23 90.9
MS.gene054543.t1 MTR_4g007560 62.500 80 23 1 1 73 1 80 2.19e-21 85.9
MS.gene054543.t1 MTR_4g007570 40.217 184 54 5 1 181 1 131 2.37e-21 85.1
MS.gene054543.t1 MTR_4g007590 53.488 86 30 2 1 76 1 86 3.61e-19 79.7
MS.gene054543.t1 MTR_4g007580 35.714 182 62 6 1 181 1 128 7.84e-13 62.8
MS.gene054543.t1 MTR_4g007640 45.304 181 64 2 1 181 1 146 1.10e-12 62.8
MS.gene054543.t1 MTR_4g007670 88.889 27 3 0 155 181 109 135 4.94e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAATGGGAGGAGGTGGATT+TGG 0.280190 4.1:+87808550 MS.gene054543:CDS
GGAGGAGGTGGAAGGGGAGG+AGG 0.294260 4.1:+87808496 MS.gene054543:CDS
ACGATAATAGGAAATTTATA+TGG 0.302449 4.1:+87808367 MS.gene054543:intron
GGAGGTGGAAGGGGAGGAGG+TGG 0.304793 4.1:+87808499 MS.gene054543:CDS
GAACAAGCACATGAGCTTAA+TGG 0.312160 4.1:+87808237 MS.gene054543:CDS
GCCTTCATCTTCATTTGAAA+TGG 0.326206 4.1:-87808193 None:intergenic
AGGGGAGGAGGTGGATTTAG+TGG 0.341806 4.1:+87808508 MS.gene054543:CDS
GGTGGAGGAAAGGGAGGAGG+TGG 0.346499 4.1:+87808574 MS.gene054543:CDS
GGAAAGGGAGGAGGTGGAAT+GGG 0.349740 4.1:+87808580 MS.gene054543:CDS
GGATTTGGAGGAGGAGTAGC+AGG 0.351628 4.1:+87808616 MS.gene054543:CDS
AAGGGAGGAGGTGGAATGGG+AGG 0.356510 4.1:+87808583 MS.gene054543:CDS
GGAGGAGGTGGAATGGGAGG+AGG 0.381242 4.1:+87808541 MS.gene054543:CDS
GGATTTGGAGGTGGAGGAAA+GGG 0.386379 4.1:+87808565 MS.gene054543:CDS
GGAGGTGGAATGGGAGGAGG+TGG 0.390133 4.1:+87808544 MS.gene054543:CDS
TTTGGAGGTGGAGGAAAGGG+AGG 0.396785 4.1:+87808568 MS.gene054543:CDS
GGAGGAGGTGGAATGGGAGG+GGG 0.398655 4.1:+87808586 MS.gene054543:CDS
CATGTGCTTGTTCATTACTC+TGG 0.404052 4.1:-87808227 None:intergenic
TCGACGGGAGGAGGTGGAAG+GGG 0.410147 4.1:+87808490 MS.gene054543:CDS
ATAGGAAATTTATATGGAAA+TGG 0.411309 4.1:+87808373 MS.gene054543:intron
GAGCATTTGAGATGATCAAT+AGG 0.414061 4.1:-87808167 None:intergenic
GCAAGCGGAGGAGGTGGAAT+GGG 0.415178 4.1:+87808535 MS.gene054543:CDS
AGGAAAGGGAGGAGGTGGAA+TGG 0.417507 4.1:+87808579 MS.gene054543:CDS
GGAGCGGCCGGTGGAATTAT+CGG 0.418438 4.1:+87808640 MS.gene054543:CDS
GGAGGTGGAGGAAAGGGAGG+AGG 0.423397 4.1:+87808571 MS.gene054543:CDS
ACGGGAGGAGGTGGAAGGGG+AGG 0.424441 4.1:+87808493 MS.gene054543:CDS
TGGATTTGGAGGTGGAGGAA+AGG 0.425116 4.1:+87808564 MS.gene054543:CDS
GGTGGGAAGGGATTTGGAGG+AGG 0.425225 4.1:+87808607 MS.gene054543:CDS
GGAGTAGCAGGTGGAGCGGC+CGG 0.432536 4.1:+87808628 MS.gene054543:CDS
GGGAGGAGGTGGAATGGGAG+GGG 0.432996 4.1:+87808585 MS.gene054543:CDS
ATGGGAGGAGGTGGATTTGG+AGG 0.433737 4.1:+87808553 MS.gene054543:CDS
ATTATCGGTGGTGGAATTGT+AGG 0.436864 4.1:+87808655 MS.gene054543:CDS
TGAAGATAAACATGAATTGT+CGG 0.447709 4.1:+87808453 MS.gene054543:CDS
GGAGGTGGATTTGGAGGTGG+AGG 0.460844 4.1:+87808559 MS.gene054543:CDS
GGAGGAGGTGGATTTGGAGG+TGG 0.476941 4.1:+87808556 MS.gene054543:CDS
AGGGAGGAGGTGGAATGGGA+GGG 0.484959 4.1:+87808584 MS.gene054543:CDS
AGTGGAGGTGCAAGCGGAGG+AGG 0.488977 4.1:+87808526 MS.gene054543:CDS
GCCATTTCAAATGAAGATGA+AGG 0.496545 4.1:+87808192 MS.gene054543:CDS
GCCGGTGGAATTATCGGTGG+TGG 0.507892 4.1:+87808646 MS.gene054543:CDS
GGAGGTGCAAGCGGAGGAGG+TGG 0.508022 4.1:+87808529 MS.gene054543:CDS
TGCAAGCGGAGGAGGTGGAA+TGG 0.508541 4.1:+87808534 MS.gene054543:CDS
TTTCGACGGGAGGAGGTGGA+AGG 0.517005 4.1:+87808488 MS.gene054543:CDS
AGCGGAGGAGGTGGAATGGG+AGG 0.541101 4.1:+87808538 MS.gene054543:CDS
GCAAATCGCGGTCACAATGG+TGG 0.544739 4.1:+87808688 MS.gene054543:CDS
TTCGACGGGAGGAGGTGGAA+GGG 0.547737 4.1:+87808489 MS.gene054543:CDS
GTAGCAGGTGGAGCGGCCGG+TGG 0.556374 4.1:+87808631 MS.gene054543:CDS
AGGAGGAGTAGCAGGTGGAG+CGG 0.582710 4.1:+87808624 MS.gene054543:CDS
CAAATCGCGGTCACAATGGT+GGG 0.586802 4.1:+87808689 MS.gene054543:CDS
GGCGGCGCAGTTGCAAATCG+CGG 0.603738 4.1:+87808676 MS.gene054543:CDS
TCCTCCCGTCGAAAACCTCA+TGG 0.608672 4.1:-87808479 None:intergenic
GGAGGAGGTGGATTTAGTGG+AGG 0.619290 4.1:+87808511 MS.gene054543:CDS
GGATTTAGTGGAGGTGCAAG+CGG 0.621029 4.1:+87808520 MS.gene054543:CDS
TCCACCACCGATAATTCCAC+CGG 0.625861 4.1:-87808647 None:intergenic
GGTCACAATGGTGGGTACAA+TGG 0.639906 4.1:+87808697 MS.gene054543:CDS
TTTAGTGGAGGTGCAAGCGG+AGG 0.644874 4.1:+87808523 MS.gene054543:CDS
ATGAATTGTCGGTTGCCATG+AGG 0.661511 4.1:+87808464 MS.gene054543:CDS
TTTGGAGGAGGAGTAGCAGG+TGG 0.662305 4.1:+87808619 MS.gene054543:CDS
ATCGGTGGTGGAATTGTAGG+CGG 0.663727 4.1:+87808658 MS.gene054543:CDS
GTTGCAAATCGCGGTCACAA+TGG 0.665083 4.1:+87808685 MS.gene054543:CDS
GCGGCCGGTGGAATTATCGG+TGG 0.683638 4.1:+87808643 MS.gene054543:CDS
GTAACATAGAAGTCTCCACA+AGG 0.724906 4.1:+87808395 MS.gene054543:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTTACTTGTTTAAAA+AGG - chr4.1:87808276-87808295 None:intergenic 15.0%
!! ACGATAATAGGAAATTTATA+TGG + chr4.1:87808367-87808386 MS.gene054543:intron 20.0%
!! ATAGGAAATTTATATGGAAA+TGG + chr4.1:87808373-87808392 MS.gene054543:intron 20.0%
!! TTTACTTGTTTAAAAAGGTA+AGG - chr4.1:87808271-87808290 None:intergenic 20.0%
!!! ACGTGTTTTTATTTTAATCT+TGG + chr4.1:87808324-87808343 MS.gene054543:intron 20.0%
! TGAAGATAAACATGAATTGT+CGG + chr4.1:87808453-87808472 MS.gene054543:CDS 25.0%
!! TTGTGTTATTCAACGATAAT+AGG + chr4.1:87808355-87808374 MS.gene054543:intron 25.0%
AATGTTGAGACACAAAAATG+AGG - chr4.1:87808757-87808776 None:intergenic 30.0%
GAACAACAAAAATGTGATTG+TGG + chr4.1:87808420-87808439 MS.gene054543:CDS 30.0%
! TGATTTTATGTATGAGCTTC+TGG + chr4.1:87808776-87808795 MS.gene054543:CDS 30.0%
!!! ATTTTTGTTGTTCAACCTTG+TGG - chr4.1:87808413-87808432 None:intergenic 30.0%
GCCATTTCAAATGAAGATGA+AGG + chr4.1:87808192-87808211 MS.gene054543:CDS 35.0%
GCCTTCATCTTCATTTGAAA+TGG - chr4.1:87808196-87808215 None:intergenic 35.0%
! GAGCATTTGAGATGATCAAT+AGG - chr4.1:87808170-87808189 None:intergenic 35.0%
CATGTGCTTGTTCATTACTC+TGG - chr4.1:87808230-87808249 None:intergenic 40.0%
GAACAAGCACATGAGCTTAA+TGG + chr4.1:87808237-87808256 MS.gene054543:CDS 40.0%
GTAACATAGAAGTCTCCACA+AGG + chr4.1:87808395-87808414 MS.gene054543:CDS 40.0%
!! ATTATCGGTGGTGGAATTGT+AGG + chr4.1:87808655-87808674 MS.gene054543:CDS 40.0%
! ATGAATTGTCGGTTGCCATG+AGG + chr4.1:87808464-87808483 MS.gene054543:CDS 45.0%
CAAATCGCGGTCACAATGGT+GGG + chr4.1:87808689-87808708 MS.gene054543:CDS 50.0%
GGATTTGGAGGTGGAGGAAA+GGG + chr4.1:87808565-87808584 MS.gene054543:CDS 50.0%
GTTGCAAATCGCGGTCACAA+TGG + chr4.1:87808685-87808704 MS.gene054543:CDS 50.0%
TCCACCACCGATAATTCCAC+CGG - chr4.1:87808650-87808669 None:intergenic 50.0%
TGGATTTGGAGGTGGAGGAA+AGG + chr4.1:87808564-87808583 MS.gene054543:CDS 50.0%
! GGATTTAGTGGAGGTGCAAG+CGG + chr4.1:87808520-87808539 MS.gene054543:CDS 50.0%
! GGTTGCCATGAGGTTTTCGA+CGG + chr4.1:87808474-87808493 MS.gene054543:CDS 50.0%
! GTTGCCATGAGGTTTTCGAC+GGG + chr4.1:87808475-87808494 MS.gene054543:CDS 50.0%
!! ATCGGTGGTGGAATTGTAGG+CGG + chr4.1:87808658-87808677 MS.gene054543:CDS 50.0%
!! GGTCACAATGGTGGGTACAA+TGG + chr4.1:87808697-87808716 MS.gene054543:CDS 50.0%
AGGAAAGGGAGGAGGTGGAA+TGG + chr4.1:87808579-87808598 MS.gene054543:CDS 55.0%
GCAAATCGCGGTCACAATGG+TGG + chr4.1:87808688-87808707 MS.gene054543:CDS 55.0%
GGAAAGGGAGGAGGTGGAAT+GGG + chr4.1:87808580-87808599 MS.gene054543:CDS 55.0%
GGATTTGGAGGAGGAGTAGC+AGG + chr4.1:87808616-87808635 MS.gene054543:CDS 55.0%
TCCTCCCGTCGAAAACCTCA+TGG - chr4.1:87808482-87808501 None:intergenic 55.0%
TTTGGAGGAGGAGTAGCAGG+TGG + chr4.1:87808619-87808638 MS.gene054543:CDS 55.0%
TTTGGAGGTGGAGGAAAGGG+AGG + chr4.1:87808568-87808587 MS.gene054543:CDS 55.0%
! AGGGGAGGAGGTGGATTTAG+TGG + chr4.1:87808508-87808527 MS.gene054543:CDS 55.0%
! ATGAGGTTTTCGACGGGAGG+AGG + chr4.1:87808481-87808500 MS.gene054543:CDS 55.0%
! ATGGGAGGAGGTGGATTTGG+AGG + chr4.1:87808553-87808572 MS.gene054543:CDS 55.0%
! GGAATGGGAGGAGGTGGATT+TGG + chr4.1:87808550-87808569 MS.gene054543:CDS 55.0%
! GGAGGAGGTGGATTTAGTGG+AGG + chr4.1:87808511-87808530 MS.gene054543:CDS 55.0%
! TTTAGTGGAGGTGCAAGCGG+AGG + chr4.1:87808523-87808542 MS.gene054543:CDS 55.0%
AAGGGAGGAGGTGGAATGGG+AGG + chr4.1:87808583-87808602 MS.gene054543:CDS 60.0%
AGGAGGAGTAGCAGGTGGAG+CGG + chr4.1:87808624-87808643 MS.gene054543:CDS 60.0%
AGGGAGGAGGTGGAATGGGA+GGG + chr4.1:87808584-87808603 MS.gene054543:CDS 60.0%
GCAAGCGGAGGAGGTGGAAT+GGG + chr4.1:87808535-87808554 MS.gene054543:CDS 60.0%
GCCGGTGGAATTATCGGTGG+TGG + chr4.1:87808646-87808665 MS.gene054543:CDS 60.0%
GGAGCGGCCGGTGGAATTAT+CGG + chr4.1:87808640-87808659 MS.gene054543:CDS 60.0%
GGTGGGAAGGGATTTGGAGG+AGG + chr4.1:87808607-87808626 MS.gene054543:CDS 60.0%
TGCAAGCGGAGGAGGTGGAA+TGG + chr4.1:87808534-87808553 MS.gene054543:CDS 60.0%
TTCGACGGGAGGAGGTGGAA+GGG + chr4.1:87808489-87808508 MS.gene054543:CDS 60.0%
TTTCGACGGGAGGAGGTGGA+AGG + chr4.1:87808488-87808507 MS.gene054543:CDS 60.0%
! AGGTTTTCGACGGGAGGAGG+TGG + chr4.1:87808484-87808503 MS.gene054543:CDS 60.0%
! GCCATGAGGTTTTCGACGGG+AGG + chr4.1:87808478-87808497 MS.gene054543:CDS 60.0%
! GGAGGAGGTGGATTTGGAGG+TGG + chr4.1:87808556-87808575 MS.gene054543:CDS 60.0%
! GGAGGTGGATTTGGAGGTGG+AGG + chr4.1:87808559-87808578 MS.gene054543:CDS 60.0%
AGCGGAGGAGGTGGAATGGG+AGG + chr4.1:87808538-87808557 MS.gene054543:CDS 65.0%
GAGGTGGAATGGGAGGGGGT+GGG + chr4.1:87808590-87808609 MS.gene054543:CDS 65.0%
GCGGCCGGTGGAATTATCGG+TGG + chr4.1:87808643-87808662 MS.gene054543:CDS 65.0%
GGAATGGGAGGGGGTGGGAA+GGG + chr4.1:87808595-87808614 MS.gene054543:CDS 65.0%
GGAGGAGGTGGAATGGGAGG+AGG + chr4.1:87808541-87808560 MS.gene054543:CDS 65.0%
GGAGGAGGTGGAATGGGAGG+GGG + chr4.1:87808541-87808560 MS.gene054543:CDS 65.0%
GGAGGGGGTGGGAAGGGATT+TGG + chr4.1:87808601-87808620 MS.gene054543:CDS 65.0%
GGAGGTGGAATGGGAGGAGG+TGG + chr4.1:87808544-87808563 MS.gene054543:CDS 65.0%
GGAGGTGGAGGAAAGGGAGG+AGG + chr4.1:87808571-87808590 MS.gene054543:CDS 65.0%
GGCGGCGCAGTTGCAAATCG+CGG + chr4.1:87808676-87808695 MS.gene054543:CDS 65.0%
GGGAGGAGGTGGAATGGGAG+GGG + chr4.1:87808585-87808604 MS.gene054543:CDS 65.0%
GGGGGTGGGAAGGGATTTGG+AGG + chr4.1:87808604-87808623 MS.gene054543:CDS 65.0%
GGTGGAGGAAAGGGAGGAGG+TGG + chr4.1:87808574-87808593 MS.gene054543:CDS 65.0%
TCGACGGGAGGAGGTGGAAG+GGG + chr4.1:87808490-87808509 MS.gene054543:CDS 65.0%
TGGAATGGGAGGGGGTGGGA+AGG + chr4.1:87808594-87808613 MS.gene054543:CDS 65.0%
! AGTGGAGGTGCAAGCGGAGG+AGG + chr4.1:87808526-87808545 MS.gene054543:CDS 65.0%
ACGGGAGGAGGTGGAAGGGG+AGG + chr4.1:87808493-87808512 MS.gene054543:CDS 70.0%
GGAGGAGGTGGAAGGGGAGG+AGG + chr4.1:87808496-87808515 MS.gene054543:CDS 70.0%
GGAGGTGGAAGGGGAGGAGG+TGG + chr4.1:87808499-87808518 MS.gene054543:CDS 70.0%
GGAGGTGGAATGGGAGGGGG+TGG + chr4.1:87808589-87808608 MS.gene054543:CDS 70.0%
GGAGTAGCAGGTGGAGCGGC+CGG + chr4.1:87808628-87808647 MS.gene054543:CDS 70.0%
! GGAGGTGCAAGCGGAGGAGG+TGG + chr4.1:87808529-87808548 MS.gene054543:CDS 70.0%
GTAGCAGGTGGAGCGGCCGG+TGG + chr4.1:87808631-87808650 MS.gene054543:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87808141 87808804 87808141 ID=MS.gene054543
chr4.1 mRNA 87808141 87808804 87808141 ID=MS.gene054543.t1;Parent=MS.gene054543
chr4.1 exon 87808141 87808258 87808141 ID=MS.gene054543.t1.exon1;Parent=MS.gene054543.t1
chr4.1 CDS 87808141 87808258 87808141 ID=cds.MS.gene054543.t1;Parent=MS.gene054543.t1
chr4.1 exon 87808377 87808804 87808377 ID=MS.gene054543.t1.exon2;Parent=MS.gene054543.t1
chr4.1 CDS 87808377 87808804 87808377 ID=cds.MS.gene054543.t1;Parent=MS.gene054543.t1
Gene Sequence

>MS.gene054543

ATGAAGGTAATATTTTTTGTATTGTTCCTATTGATCATCTCAAATGCTCAAGCCATTTCAAATGAAGATGAAGGCAAAAACATAATCCAGAGTAATGAACAAGCACATGAGCTTAATGGTACAATTTCCTTACCTTTTTAAACAAGTAAAAACTTAGTTAACGTTTCACACATTTGTGAGCATACGTGTTTTTATTTTAATCTTGGTTTTATAATTGTGTTATTCAACGATAATAGGAAATTTATATGGAAATGGTAACATAGAAGTCTCCACAAGGTTGAACAACAAAAATGTGATTGTGGCAAGTAAAAATGAAGATAAACATGAATTGTCGGTTGCCATGAGGTTTTCGACGGGAGGAGGTGGAAGGGGAGGAGGTGGATTTAGTGGAGGTGCAAGCGGAGGAGGTGGAATGGGAGGAGGTGGATTTGGAGGTGGAGGAAAGGGAGGAGGTGGAATGGGAGGGGGTGGGAAGGGATTTGGAGGAGGAGTAGCAGGTGGAGCGGCCGGTGGAATTATCGGTGGTGGAATTGTAGGCGGCGCAGTTGCAAATCGCGGTCACAATGGTGGGTACAATGGCACTCATAATTCTGCAACAACATTATCTGCAGTGCCTCATTTTTGTGTCTCAACATTGATTTTATGTATGAGCTTCTGGCTTTAG

Protein sequence

>MS.gene054543.t1

MKVIFFVLFLLIISNAQAISNEDEGKNIIQSNEQAHELNGNLYGNGNIEVSTRLNNKNVIVASKNEDKHELSVAMRFSTGGGGRGGGGFSGGASGGGGMGGGGFGGGGKGGGGMGGGGKGFGGGVAGGAAGGIIGGGIVGGAVANRGHNGGYNGTHNSATTLSAVPHFCVSTLILCMSFWL