AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23945


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23945.t1 MTR_2g103750 91.935 62 5 0 10 71 74 135 2.43e-36 119
MS.gene23945.t1 MTR_4g010530 61.364 44 16 1 28 70 80 123 9.47e-12 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 8 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAAGCCATTCTATTTGC+AGG 0.298881 2.1:+1886204 None:intergenic
ATAGAATGGCTTTGTATAAC+GGG 0.410953 2.1:-1886197 MS.gene23945:CDS
TGCGAAGGAAGTAAGAGAAA+AGG 0.427100 2.1:-1886313 MS.gene23945:CDS
GCGAAGGAAGTAAGAGAAAA+GGG 0.441362 2.1:-1886312 MS.gene23945:CDS
AATAGAATGGCTTTGTATAA+CGG 0.457196 2.1:-1886198 MS.gene23945:CDS
TAGATTTGAAGAAAGTGCGA+AGG 0.517830 2.1:-1886328 MS.gene23945:CDS
TGGAATTCCTGCAAATAGAA+TGG 0.533064 2.1:-1886211 MS.gene23945:CDS
AGTGAAACAACGAAAACTCG+TGG 0.634965 2.1:-1886231 MS.gene23945:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGAATGGCTTTGTATAA+CGG - chr2.1:1886323-1886342 MS.gene23945:CDS 25.0%
TAGCAAAAAAAGAGAAACAG+AGG - chr2.1:1886237-1886256 MS.gene23945:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG - chr2.1:1886324-1886343 MS.gene23945:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG - chr2.1:1886334-1886353 MS.gene23945:CDS 30.0%
TACAAAGCCATTCTATTTGC+AGG + chr2.1:1886320-1886339 None:intergenic 35.0%
TGGAATTCCTGCAAATAGAA+TGG - chr2.1:1886310-1886329 MS.gene23945:CDS 35.0%
! TAGATTTGAAGAAAGTGCGA+AGG - chr2.1:1886193-1886212 MS.gene23945:CDS 35.0%
!! CTTTGTATAACGGGATTTTG+AGG - chr2.1:1886333-1886352 MS.gene23945:CDS 35.0%
AGTGAAACAACGAAAACTCG+TGG - chr2.1:1886290-1886309 MS.gene23945:CDS 40.0%
GCGAAGGAAGTAAGAGAAAA+GGG - chr2.1:1886209-1886228 MS.gene23945:CDS 40.0%
TGCGAAGGAAGTAAGAGAAA+AGG - chr2.1:1886208-1886227 MS.gene23945:CDS 40.0%
!! TTTTGAGGGATCGTGTGCAC+AGG - chr2.1:1886348-1886367 MS.gene23945:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 1886164 1886379 1886164 ID=MS.gene23945
chr2.1 mRNA 1886164 1886379 1886164 ID=MS.gene23945.t1;Parent=MS.gene23945
chr2.1 exon 1886164 1886379 1886164 ID=MS.gene23945.t1.exon1;Parent=MS.gene23945.t1
chr2.1 CDS 1886164 1886379 1886164 ID=cds.MS.gene23945.t1;Parent=MS.gene23945.t1
Gene Sequence

>MS.gene23945

ATGAATACAAAGAAAAATAAAAAGAAAGTTAGATTTGAAGAAAGTGCGAAGGAAGTAAGAGAAAAGGGTGAGATAGCAAAAAAAGAGAAACAGAGGAAACGAAACAGAGTACATAGCAACTGCAGAAGTGAAACAACGAAAACTCGTGGAATTCCTGCAAATAGAATGGCTTTGTATAACGGGATTTTGAGGGATCGTGTGCACAGGATTGAGTGA

Protein sequence

>MS.gene23945.t1

MNTKKNKKKVRFEESAKEVREKGEIAKKEKQRKRNRVHSNCRSETTKTRGIPANRMALYNGILRDRVHRIE