AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24905


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24905.t1 MTR_4g011960 97.170 212 6 0 1 212 44 255 5.75e-154 427
MS.gene24905.t1 MTR_2g090045 32.692 208 131 3 4 208 59 260 6.03e-37 130
MS.gene24905.t1 MTR_5g024800 36.970 165 97 2 47 208 63 223 5.96e-36 126
MS.gene24905.t1 MTR_2g090035 30.323 155 90 4 56 208 59 197 9.63e-21 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24905.t1 AT1G04940 70.707 198 57 1 15 212 78 274 7.12e-105 303
MS.gene24905.t1 AT1G04945 67.598 179 57 1 15 193 78 255 2.07e-85 265
MS.gene24905.t1 AT4G03320 34.906 212 126 4 2 208 67 271 3.98e-40 139

Find 58 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAATTGGAATGCATTTC+TGG 0.102228 4.1:+83913406 MS.gene24905:CDS
TTCACCCTTCATATGGTATT+TGG 0.132090 4.1:-83913533 None:intergenic
GATGCCACTGGGGTTCTATT+GGG 0.221927 4.1:+83913383 MS.gene24905:CDS
GGATGCCACTGGGGTTCTAT+TGG 0.233429 4.1:+83913382 MS.gene24905:CDS
GGGTTCTATTGGGGTAAAAT+TGG 0.263490 4.1:+83913393 MS.gene24905:CDS
GAGATAGCCTTGCAAGTTAC+AGG 0.289946 4.1:+83913345 MS.gene24905:CDS
CTTCGCTGCGCAAGTCTTGT+AGG 0.317793 4.1:+83912998 MS.gene24905:CDS
AACTCGAATGCCTCGATAAA+CGG 0.330825 4.1:-83913177 None:intergenic
TGCGTTAGGTGTGCCCTTAC+TGG 0.339865 4.1:+83913468 MS.gene24905:CDS
GGCACACCTAACGCACTCTA+TGG 0.372183 4.1:-83913460 None:intergenic
CTATGGAAGTAAAGAGATAA+AGG 0.374679 4.1:-83913443 None:intergenic
TACATATTTCACCCTTCATA+TGG 0.376846 4.1:-83913540 None:intergenic
TGTGAGCCGTTGGATGCCAC+TGG 0.406570 4.1:+83913371 MS.gene24905:CDS
ACTCGAATGCCTCGATAAAC+GGG 0.406627 4.1:-83913176 None:intergenic
AGTGTTCTCCACCACCATCT+TGG 0.422947 4.1:-83913084 None:intergenic
TTACTTCCATAGAGTGCGTT+AGG 0.425451 4.1:+83913454 MS.gene24905:CDS
TTATCTCTTGCCTATCCATC+AGG 0.444896 4.1:+83913119 MS.gene24905:CDS
GATGGATAGGCAAGAGATAA+GGG 0.454516 4.1:-83913116 None:intergenic
ATGCCACTGGGGTTCTATTG+GGG 0.470377 4.1:+83913384 MS.gene24905:CDS
GTGAGCCGTTGGATGCCACT+GGG 0.473897 4.1:+83913372 MS.gene24905:CDS
TATGCTGCATCACAGACAAA+AGG 0.482262 4.1:-83913507 None:intergenic
TGATGGATAGGCAAGAGATA+AGG 0.482592 4.1:-83913117 None:intergenic
TTCGCTGCGCAAGTCTTGTA+GGG 0.489910 4.1:+83912999 MS.gene24905:CDS
TATATCCAAATACCATATGA+AGG 0.493979 4.1:+83913528 MS.gene24905:CDS
ATGAAGGAAGGCTCATCTGC+TGG 0.500226 4.1:+83913033 MS.gene24905:CDS
TTCAGATTTCACGTTGCCGT+CGG 0.502375 4.1:+83913312 MS.gene24905:CDS
TAGGGCCCCTCGAGCAATGA+AGG 0.513500 4.1:+83913017 MS.gene24905:CDS
CAATTGGCAGTCTACCTAGA+TGG 0.514868 4.1:+83913223 MS.gene24905:CDS
GAGCCTTCCTTCATTGCTCG+AGG 0.525595 4.1:-83913024 None:intergenic
TATCCGCATACATACCAGTA+AGG 0.527566 4.1:-83913482 None:intergenic
AGCCTTCCTTCATTGCTCGA+GGG 0.536520 4.1:-83913023 None:intergenic
ATGCTGCATCACAGACAAAA+GGG 0.543856 4.1:-83913506 None:intergenic
CTACAAGACTTGCGCAGCGA+AGG 0.549633 4.1:-83912997 None:intergenic
TTACAGGGCTTGTGAGCCGT+TGG 0.554372 4.1:+83913361 MS.gene24905:CDS
TAGCATACATCCACACCTGA+TGG 0.557726 4.1:-83913134 None:intergenic
AGATAGCCTTGCAAGTTACA+GGG 0.572582 4.1:+83913346 MS.gene24905:CDS
TACATCCACACCTGATGGAT+AGG 0.579710 4.1:-83913129 None:intergenic
CCAGAAAACCAAGATGGTGG+TGG 0.582114 4.1:+83913076 MS.gene24905:CDS
TATGCTATGAGAGACCATCT+AGG 0.591244 4.1:-83913237 None:intergenic
CACAAGCCCTGTAACTTGCA+AGG 0.594415 4.1:-83913352 None:intergenic
ACGTGAAATCTGAAGAAATG+AGG 0.595610 4.1:-83913303 None:intergenic
TTGCGCAGCGAAGGTAACAC+AGG 0.597116 4.1:-83912988 None:intergenic
TTGCGTATCTTACCATTGTG+AGG 0.599587 4.1:+83913268 MS.gene24905:CDS
GCCTTCCTTCATTGCTCGAG+GGG 0.601540 4.1:-83913022 None:intergenic
TCTTGCCTATCCATCAGGTG+TGG 0.604602 4.1:+83913124 MS.gene24905:CDS
GCCCCTCGAGCAATGAAGGA+AGG 0.617175 4.1:+83913021 MS.gene24905:CDS
CAATCTTCACCCGTTTATCG+AGG 0.617293 4.1:+83913167 MS.gene24905:CDS
ATATCCAAATACCATATGAA+GGG 0.626189 4.1:+83913529 MS.gene24905:CDS
CGATGACCAGAAAACCAAGA+TGG 0.627736 4.1:+83913070 MS.gene24905:CDS
AGAACCCCAGTGGCATCCAA+CGG 0.641127 4.1:-83913377 None:intergenic
CCATTGTGAGGAGAAAAGAA+TGG 0.645047 4.1:+83913280 MS.gene24905:CDS
TGCCCTTACTGGTATGTATG+CGG 0.645316 4.1:+83913479 MS.gene24905:CDS
ATCCGCATACATACCAGTAA+GGG 0.651307 4.1:-83913481 None:intergenic
TGACCAGAAAACCAAGATGG+TGG 0.673799 4.1:+83913073 MS.gene24905:CDS
TGCGCAGCGAAGGTAACACA+GGG 0.711989 4.1:-83912987 None:intergenic
TATCTCGATTAACATTCCGA+CGG 0.723472 4.1:-83913328 None:intergenic
TGAGCCGTTGGATGCCACTG+GGG 0.729430 4.1:+83913373 MS.gene24905:CDS
TTACCCCAATAGAACCCCAG+TGG 0.740833 4.1:-83913387 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATCCAAATACCATATGAA+GGG + chr4.1:83913529-83913548 MS.gene24905:CDS 25.0%
! TATATCCAAATACCATATGA+AGG + chr4.1:83913528-83913547 MS.gene24905:CDS 25.0%
! TGCCAATTGACATAAAAAAA+GGG - chr4.1:83913212-83913231 None:intergenic 25.0%
!!! TACCCTTTTTTTATGTCAAT+TGG + chr4.1:83913207-83913226 MS.gene24905:CDS 25.0%
CTATGGAAGTAAAGAGATAA+AGG - chr4.1:83913446-83913465 None:intergenic 30.0%
CTGCCAATTGACATAAAAAA+AGG - chr4.1:83913213-83913232 None:intergenic 30.0%
GTAAAATTGGAATGCATTTC+TGG + chr4.1:83913406-83913425 MS.gene24905:CDS 30.0%
ACGTGAAATCTGAAGAAATG+AGG - chr4.1:83913306-83913325 None:intergenic 35.0%
TATCTCGATTAACATTCCGA+CGG - chr4.1:83913331-83913350 None:intergenic 35.0%
!! ATCAAGCCTACTTTTAAGTG+GGG + chr4.1:83912939-83912958 MS.gene24905:CDS 35.0%
!! CATCAAGCCTACTTTTAAGT+GGG + chr4.1:83912938-83912957 MS.gene24905:CDS 35.0%
AACTCGAATGCCTCGATAAA+CGG - chr4.1:83913180-83913199 None:intergenic 40.0%
ATCCGCATACATACCAGTAA+GGG - chr4.1:83913484-83913503 None:intergenic 40.0%
ATGCTGCATCACAGACAAAA+GGG - chr4.1:83913509-83913528 None:intergenic 40.0%
CCATTGTGAGGAGAAAAGAA+TGG + chr4.1:83913280-83913299 MS.gene24905:CDS 40.0%
GATGGATAGGCAAGAGATAA+GGG - chr4.1:83913119-83913138 None:intergenic 40.0%
GGGTTCTATTGGGGTAAAAT+TGG + chr4.1:83913393-83913412 MS.gene24905:CDS 40.0%
TATCCGCATACATACCAGTA+AGG - chr4.1:83913485-83913504 None:intergenic 40.0%
TATGCTATGAGAGACCATCT+AGG - chr4.1:83913240-83913259 None:intergenic 40.0%
TATGCTGCATCACAGACAAA+AGG - chr4.1:83913510-83913529 None:intergenic 40.0%
TTACTTCCATAGAGTGCGTT+AGG + chr4.1:83913454-83913473 MS.gene24905:CDS 40.0%
TTATCTCTTGCCTATCCATC+AGG + chr4.1:83913119-83913138 MS.gene24905:CDS 40.0%
TTGCGTATCTTACCATTGTG+AGG + chr4.1:83913268-83913287 MS.gene24905:CDS 40.0%
TTTGACCCCCACTTAAAAGT+AGG - chr4.1:83912948-83912967 None:intergenic 40.0%
! AGATAGCCTTGCAAGTTACA+GGG + chr4.1:83913346-83913365 MS.gene24905:CDS 40.0%
! CCATTCTTTTCTCCTCACAA+TGG - chr4.1:83913283-83913302 None:intergenic 40.0%
! TGATGGATAGGCAAGAGATA+AGG - chr4.1:83913120-83913139 None:intergenic 40.0%
!! CATCTTGGTTTTCTGGTCAT+CGG - chr4.1:83913072-83913091 None:intergenic 40.0%
!! GCATCAAGCCTACTTTTAAG+TGG + chr4.1:83912937-83912956 MS.gene24905:CDS 40.0%
!! TCAAGCCTACTTTTAAGTGG+GGG + chr4.1:83912940-83912959 MS.gene24905:CDS 40.0%
ACTCGAATGCCTCGATAAAC+GGG - chr4.1:83913179-83913198 None:intergenic 45.0%
CAATCTTCACCCGTTTATCG+AGG + chr4.1:83913167-83913186 MS.gene24905:CDS 45.0%
CAATTGGCAGTCTACCTAGA+TGG + chr4.1:83913223-83913242 MS.gene24905:CDS 45.0%
CGATGACCAGAAAACCAAGA+TGG + chr4.1:83913070-83913089 MS.gene24905:CDS 45.0%
TAGCATACATCCACACCTGA+TGG - chr4.1:83913137-83913156 None:intergenic 45.0%
TGACCAGAAAACCAAGATGG+TGG + chr4.1:83913073-83913092 MS.gene24905:CDS 45.0%
TGCCCTTACTGGTATGTATG+CGG + chr4.1:83913479-83913498 MS.gene24905:CDS 45.0%
TTCAGATTTCACGTTGCCGT+CGG + chr4.1:83913312-83913331 MS.gene24905:CDS 45.0%
! GAGATAGCCTTGCAAGTTAC+AGG + chr4.1:83913345-83913364 MS.gene24905:CDS 45.0%
! TACATCCACACCTGATGGAT+AGG - chr4.1:83913132-83913151 None:intergenic 45.0%
AGCCTTCCTTCATTGCTCGA+GGG - chr4.1:83913026-83913045 None:intergenic 50.0%
AGTGTTCTCCACCACCATCT+TGG - chr4.1:83913087-83913106 None:intergenic 50.0%
ATGAAGGAAGGCTCATCTGC+TGG + chr4.1:83913033-83913052 MS.gene24905:CDS 50.0%
ATGCCACTGGGGTTCTATTG+GGG + chr4.1:83913384-83913403 MS.gene24905:CDS 50.0%
GATGCCACTGGGGTTCTATT+GGG + chr4.1:83913383-83913402 MS.gene24905:CDS 50.0%
TCTTGCCTATCCATCAGGTG+TGG + chr4.1:83913124-83913143 MS.gene24905:CDS 50.0%
TTACCCCAATAGAACCCCAG+TGG - chr4.1:83913390-83913409 None:intergenic 50.0%
! CACAAGCCCTGTAACTTGCA+AGG - chr4.1:83913355-83913374 None:intergenic 50.0%
! CCAGAAAACCAAGATGGTGG+TGG + chr4.1:83913076-83913095 MS.gene24905:CDS 50.0%
! TTCGCTGCGCAAGTCTTGTA+GGG + chr4.1:83912999-83913018 MS.gene24905:CDS 50.0%
!! CCACCACCATCTTGGTTTTC+TGG - chr4.1:83913079-83913098 None:intergenic 50.0%
!! CTTGGTTTTCTGGTCATCGG+AGG - chr4.1:83913069-83913088 None:intergenic 50.0%
AGAACCCCAGTGGCATCCAA+CGG - chr4.1:83913380-83913399 None:intergenic 55.0%
CTACAAGACTTGCGCAGCGA+AGG - chr4.1:83913000-83913019 None:intergenic 55.0%
GAGCCTTCCTTCATTGCTCG+AGG - chr4.1:83913027-83913046 None:intergenic 55.0%
GCCTTCCTTCATTGCTCGAG+GGG - chr4.1:83913025-83913044 None:intergenic 55.0%
GGATGCCACTGGGGTTCTAT+TGG + chr4.1:83913382-83913401 MS.gene24905:CDS 55.0%
GGCACACCTAACGCACTCTA+TGG - chr4.1:83913463-83913482 None:intergenic 55.0%
TGCGCAGCGAAGGTAACACA+GGG - chr4.1:83912990-83913009 None:intergenic 55.0%
TGCGTTAGGTGTGCCCTTAC+TGG + chr4.1:83913468-83913487 MS.gene24905:CDS 55.0%
TTACAGGGCTTGTGAGCCGT+TGG + chr4.1:83913361-83913380 MS.gene24905:CDS 55.0%
TTGCGCAGCGAAGGTAACAC+AGG - chr4.1:83912991-83913010 None:intergenic 55.0%
! CTTCGCTGCGCAAGTCTTGT+AGG + chr4.1:83912998-83913017 MS.gene24905:CDS 55.0%
GCCCCTCGAGCAATGAAGGA+AGG + chr4.1:83913021-83913040 MS.gene24905:CDS 60.0%
GTGAGCCGTTGGATGCCACT+GGG + chr4.1:83913372-83913391 MS.gene24905:CDS 60.0%
TAGGGCCCCTCGAGCAATGA+AGG + chr4.1:83913017-83913036 MS.gene24905:CDS 60.0%
TGAGCCGTTGGATGCCACTG+GGG + chr4.1:83913373-83913392 MS.gene24905:CDS 60.0%
TGTGAGCCGTTGGATGCCAC+TGG + chr4.1:83913371-83913390 MS.gene24905:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 83912913 83913554 83912913 ID=MS.gene24905
chr4.1 mRNA 83912913 83913554 83912913 ID=MS.gene24905.t1;Parent=MS.gene24905
chr4.1 exon 83912913 83913554 83912913 ID=MS.gene24905.t1.exon1;Parent=MS.gene24905.t1
chr4.1 CDS 83912913 83913554 83912913 ID=cds.MS.gene24905.t1;Parent=MS.gene24905.t1
Gene Sequence

>MS.gene24905

ATGGCATACACAGACATGTCTTCTGCATCAAGCCTACTTTTAAGTGGGGGTCAAAATCTTCTATCTCGTACTATCCCTGTGTTACCTTCGCTGCGCAAGTCTTGTAGGGCCCCTCGAGCAATGAAGGAAGGCTCATCTGCTGGCTTTAAGTTTCCTCCGATGACCAGAAAACCAAGATGGTGGTGGAGAACACTAGCATGCATCCCTTATCTCTTGCCTATCCATCAGGTGTGGATGTATGCTAAGACAGCATACAATCTTCACCCGTTTATCGAGGCATTCGAGTTCTTAACGTACCCTTTTTTTATGTCAATTGGCAGTCTACCTAGATGGTCTCTCATAGCATACTTCTTGATTGCGTATCTTACCATTGTGAGGAGAAAAGAATGGCCTCATTTCTTCAGATTTCACGTTGCCGTCGGAATGTTAATCGAGATAGCCTTGCAAGTTACAGGGCTTGTGAGCCGTTGGATGCCACTGGGGTTCTATTGGGGTAAAATTGGAATGCATTTCTGGACTACTGCATTTTTCCTTTATCTCTTTACTTCCATAGAGTGCGTTAGGTGTGCCCTTACTGGTATGTATGCGGATATCCCTTTTGTCTGTGATGCAGCATATATCCAAATACCATATGAAGGGTGA

Protein sequence

>MS.gene24905.t1

MAYTDMSSASSLLLSGGQNLLSRTIPVLPSLRKSCRAPRAMKEGSSAGFKFPPMTRKPRWWWRTLACIPYLLPIHQVWMYAKTAYNLHPFIEAFEFLTYPFFMSIGSLPRWSLIAYFLIAYLTIVRRKEWPHFFRFHVAVGMLIEIALQVTGLVSRWMPLGFYWGKIGMHFWTTAFFLYLFTSIECVRCALTGMYADIPFVCDAAYIQIPYEG