Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25436.t1 | XP_013445749.1 | 100 | 233 | 0 | 0 | 1 | 233 | 50 | 282 | 8.80E-105 | 389.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25436.t1 | Q8LC03 | 65.1 | 232 | 77 | 2 | 6 | 233 | 63 | 294 | 6.7e-53 | 208.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25436.t1 | A0A072U1W4 | 100.0 | 233 | 0 | 0 | 1 | 233 | 50 | 282 | 6.4e-105 | 389.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene25436.t1 | TF | HB-HD-ZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene25436 | 0.815891 | 7.33E-52 | -1.69E-46 |
| MS.gene049190 | MS.gene25436 | 0.839569 | 1.47E-57 | -1.69E-46 |
| MS.gene049328 | MS.gene25436 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene049340 | MS.gene25436 | 0.804408 | 2.19E-49 | -1.69E-46 |
| MS.gene049341 | MS.gene25436 | 0.855119 | 7.81E-62 | -1.69E-46 |
| MS.gene049404 | MS.gene25436 | 0.819583 | 1.08E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene25436 | 0.817057 | 4.02E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene25436 | 0.828599 | 8.22E-55 | -1.69E-46 |
| MS.gene049808 | MS.gene25436 | 0.813711 | 2.23E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene25436 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene25436 | 0.846156 | 2.59E-59 | -1.69E-46 |
| MS.gene050241 | MS.gene25436 | 0.803385 | 3.58E-49 | -1.69E-46 |
| MS.gene050586 | MS.gene25436 | 0.800478 | 1.41E-48 | -1.69E-46 |
| MS.gene050689 | MS.gene25436 | 0.812604 | 3.90E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene25436 | 0.858347 | 8.77E-63 | -1.69E-46 |
| MS.gene050808 | MS.gene25436 | 0.819873 | 9.24E-53 | -1.69E-46 |
| MS.gene050901 | MS.gene25436 | 0.813789 | 2.14E-51 | -1.69E-46 |
| MS.gene051102 | MS.gene25436 | 0.813405 | 2.60E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene25436 | 0.809081 | 2.26E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene25436 | 0.821955 | 3.07E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene25436 | 0.825355 | 4.90E-54 | -1.69E-46 |
| MS.gene05157 | MS.gene25436 | 0.843492 | 1.36E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene25436 | 0.868338 | 7.03E-66 | -1.69E-46 |
| MS.gene051720 | MS.gene25436 | 0.853365 | 2.51E-61 | -1.69E-46 |
| MS.gene051744 | MS.gene25436 | 0.818442 | 1.96E-52 | -1.69E-46 |
| MS.gene051957 | MS.gene25436 | 0.800866 | 1.18E-48 | -1.69E-46 |
| MS.gene052020 | MS.gene25436 | 0.805914 | 1.06E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene25436 | 0.84439 | 7.79E-59 | -1.69E-46 |
| MS.gene052239 | MS.gene25436 | -0.806656 | 7.40E-50 | -1.69E-46 |
| MS.gene052514 | MS.gene25436 | 0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene25436 | 0.865717 | 4.82E-65 | -1.69E-46 |
| MS.gene052611 | MS.gene25436 | 0.819627 | 1.05E-52 | -1.69E-46 |
| MS.gene052612 | MS.gene25436 | 0.802588 | 5.23E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene25436 | 0.851058 | 1.14E-60 | -1.69E-46 |
| MS.gene052732 | MS.gene25436 | 0.848424 | 6.18E-60 | -1.69E-46 |
| MS.gene052879 | MS.gene25436 | 0.826369 | 2.82E-54 | -1.69E-46 |
| MS.gene052880 | MS.gene25436 | 0.81253 | 4.05E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene25436 | 0.815039 | 1.13E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene25436 | 0.872848 | 2.32E-67 | -1.69E-46 |
| MS.gene053174 | MS.gene25436 | 0.839969 | 1.15E-57 | -1.69E-46 |
| MS.gene053581 | MS.gene25436 | 0.85503 | 8.29E-62 | -1.69E-46 |
| MS.gene053597 | MS.gene25436 | 0.809597 | 1.75E-50 | -1.69E-46 |
| MS.gene053599 | MS.gene25436 | 0.844454 | 7.49E-59 | -1.69E-46 |
| MS.gene05364 | MS.gene25436 | 0.807498 | 4.91E-50 | -1.69E-46 |
| MS.gene053883 | MS.gene25436 | 0.81523 | 1.03E-51 | -1.69E-46 |
| MS.gene054550 | MS.gene25436 | 0.838596 | 2.62E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene25436 | 0.873352 | 1.57E-67 | -1.69E-46 |
| MS.gene05462 | MS.gene25436 | 0.816509 | 5.33E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene25436 | 0.817427 | 3.32E-52 | -1.69E-46 |
| MS.gene054791 | MS.gene25436 | 0.803802 | 2.93E-49 | -1.69E-46 |
| MS.gene055144 | MS.gene25436 | 0.823704 | 1.20E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene25436 | 0.870669 | 1.23E-66 | -1.69E-46 |
| MS.gene055466 | MS.gene25436 | 0.862434 | 5.08E-64 | -1.69E-46 |
| MS.gene055468 | MS.gene25436 | 0.851657 | 7.69E-61 | -1.69E-46 |
| MS.gene055469 | MS.gene25436 | 0.847983 | 8.19E-60 | -1.69E-46 |
| MS.gene055512 | MS.gene25436 | 0.832346 | 9.96E-56 | -1.69E-46 |
| MS.gene055537 | MS.gene25436 | 0.838525 | 2.73E-57 | -1.69E-46 |
| MS.gene055580 | MS.gene25436 | -0.858171 | 9.89E-63 | -1.69E-46 |
| MS.gene055890 | MS.gene25436 | 0.955199 | 5.25E-113 | -1.69E-46 |
| MS.gene055891 | MS.gene25436 | 0.936171 | 2.66E-97 | -1.69E-46 |
| MS.gene056043 | MS.gene25436 | -0.837517 | 4.96E-57 | -1.69E-46 |
| MS.gene056308 | MS.gene25436 | 0.822433 | 2.38E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene25436 | 0.805642 | 1.21E-49 | -1.69E-46 |
| MS.gene056507 | MS.gene25436 | 0.81712 | 3.89E-52 | -1.69E-46 |
| MS.gene056553 | MS.gene25436 | 0.83418 | 3.48E-56 | -1.69E-46 |
| MS.gene056584 | MS.gene25436 | 0.801534 | 8.60E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene25436 | 0.801801 | 7.58E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene25436 | 0.81382 | 2.11E-51 | -1.69E-46 |
| MS.gene058087 | MS.gene25436 | 0.81408 | 1.85E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene25436 | 0.814795 | 1.28E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene25436 | 0.808172 | 3.53E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene25436 | 0.801417 | 9.09E-49 | -1.69E-46 |
| MS.gene058945 | MS.gene25436 | 0.827424 | 1.58E-54 | -1.69E-46 |
| MS.gene05894 | MS.gene25436 | 0.820669 | 6.07E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene25436 | 0.841376 | 4.93E-58 | -1.69E-46 |
| MS.gene059154 | MS.gene25436 | 0.803481 | 3.42E-49 | -1.69E-46 |
| MS.gene05978 | MS.gene25436 | 0.815707 | 8.06E-52 | -1.69E-46 |
| MS.gene059937 | MS.gene25436 | 0.839463 | 1.56E-57 | -1.69E-46 |
| MS.gene060194 | MS.gene25436 | 0.824049 | 9.96E-54 | -1.69E-46 |
| MS.gene060519 | MS.gene25436 | 0.831919 | 1.27E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene25436 | 0.865965 | 4.03E-65 | -1.69E-46 |
| MS.gene060603 | MS.gene25436 | 0.822619 | 2.15E-53 | -1.69E-46 |
| MS.gene060918 | MS.gene25436 | 0.810465 | 1.14E-50 | -1.69E-46 |
| MS.gene061176 | MS.gene25436 | 0.832092 | 1.15E-55 | -1.69E-46 |
| MS.gene061177 | MS.gene25436 | 0.829934 | 3.90E-55 | -1.69E-46 |
| MS.gene061178 | MS.gene25436 | 0.831724 | 1.42E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25436.t1 | MTR_8g468210 | 100.000 | 233 | 0 | 0 | 1 | 233 | 50 | 282 | 4.24e-173 | 477 |
| MS.gene25436.t1 | MTR_5g039000 | 85.232 | 237 | 30 | 4 | 1 | 233 | 48 | 283 | 2.15e-132 | 374 |
| MS.gene25436.t1 | MTR_7g010020 | 55.745 | 235 | 84 | 7 | 1 | 226 | 48 | 271 | 1.86e-74 | 227 |
| MS.gene25436.t1 | MTR_6g011610 | 53.689 | 244 | 70 | 8 | 1 | 230 | 58 | 272 | 7.52e-74 | 226 |
| MS.gene25436.t1 | MTR_6g011610 | 55.909 | 220 | 65 | 6 | 15 | 230 | 62 | 253 | 7.85e-73 | 222 |
| MS.gene25436.t1 | MTR_6g007647 | 64.000 | 100 | 36 | 0 | 24 | 123 | 29 | 128 | 8.95e-40 | 137 |
| MS.gene25436.t1 | MTR_6g007647 | 67.021 | 94 | 31 | 0 | 27 | 120 | 66 | 159 | 3.16e-39 | 137 |
| MS.gene25436.t1 | MTR_3g080100 | 48.276 | 174 | 67 | 4 | 15 | 173 | 7 | 172 | 3.23e-38 | 132 |
| MS.gene25436.t1 | MTR_3g080100 | 49.689 | 161 | 62 | 3 | 15 | 164 | 46 | 198 | 5.17e-38 | 133 |
| MS.gene25436.t1 | MTR_5g038280 | 66.667 | 96 | 32 | 0 | 28 | 123 | 87 | 182 | 2.08e-37 | 133 |
| MS.gene25436.t1 | MTR_4g107650 | 58.475 | 118 | 46 | 2 | 3 | 120 | 73 | 187 | 3.41e-37 | 133 |
| MS.gene25436.t1 | MTR_3g086790 | 56.911 | 123 | 46 | 2 | 1 | 123 | 40 | 155 | 3.96e-37 | 132 |
| MS.gene25436.t1 | MTR_8g089895 | 62.245 | 98 | 37 | 0 | 26 | 123 | 52 | 149 | 1.41e-36 | 130 |
| MS.gene25436.t1 | MTR_8g469430 | 65.625 | 96 | 33 | 0 | 28 | 123 | 89 | 184 | 1.43e-36 | 130 |
| MS.gene25436.t1 | MTR_7g103340 | 47.692 | 130 | 52 | 2 | 3 | 123 | 29 | 151 | 8.80e-33 | 119 |
| MS.gene25436.t1 | MTR_1g061660 | 52.830 | 106 | 41 | 2 | 16 | 121 | 49 | 145 | 3.74e-28 | 107 |
| MS.gene25436.t1 | MTR_3g092150 | 53.846 | 91 | 42 | 0 | 30 | 120 | 40 | 130 | 1.89e-27 | 105 |
| MS.gene25436.t1 | MTR_8g026960 | 38.000 | 150 | 82 | 3 | 35 | 173 | 41 | 190 | 3.96e-25 | 99.4 |
| MS.gene25436.t1 | MTR_4g100550 | 44.444 | 108 | 57 | 2 | 10 | 114 | 99 | 206 | 6.68e-20 | 86.3 |
| MS.gene25436.t1 | MTR_5g019680 | 47.778 | 90 | 47 | 0 | 28 | 117 | 53 | 142 | 1.76e-19 | 83.6 |
| MS.gene25436.t1 | MTR_5g019650 | 46.809 | 94 | 50 | 0 | 28 | 121 | 53 | 146 | 1.82e-19 | 84.0 |
| MS.gene25436.t1 | MTR_4g097600 | 45.263 | 95 | 52 | 0 | 20 | 114 | 119 | 213 | 9.30e-19 | 82.8 |
| MS.gene25436.t1 | MTR_7g093430 | 46.939 | 98 | 48 | 1 | 28 | 125 | 160 | 253 | 3.20e-18 | 82.0 |
| MS.gene25436.t1 | MTR_1g054285 | 42.609 | 115 | 62 | 1 | 11 | 125 | 137 | 247 | 5.08e-18 | 81.3 |
| MS.gene25436.t1 | MTR_5g014890 | 44.000 | 100 | 56 | 0 | 20 | 119 | 115 | 214 | 7.91e-18 | 80.5 |
| MS.gene25436.t1 | MTR_5g013010 | 40.708 | 113 | 66 | 1 | 3 | 114 | 110 | 222 | 1.05e-17 | 80.5 |
| MS.gene25436.t1 | MTR_2g061030 | 39.669 | 121 | 69 | 1 | 5 | 125 | 177 | 293 | 1.44e-17 | 80.5 |
| MS.gene25436.t1 | MTR_8g006705 | 48.276 | 87 | 45 | 0 | 28 | 114 | 146 | 232 | 2.53e-17 | 79.3 |
| MS.gene25436.t1 | MTR_4g126900 | 45.882 | 85 | 46 | 0 | 31 | 115 | 91 | 175 | 5.65e-17 | 77.0 |
| MS.gene25436.t1 | MTR_1g017090 | 45.455 | 88 | 48 | 0 | 28 | 115 | 130 | 217 | 1.54e-16 | 77.0 |
| MS.gene25436.t1 | MTR_4g084750 | 43.434 | 99 | 52 | 1 | 28 | 126 | 65 | 159 | 1.92e-15 | 72.4 |
| MS.gene25436.t1 | MTR_3g103590 | 48.611 | 72 | 37 | 0 | 28 | 99 | 145 | 216 | 3.64e-14 | 70.5 |
| MS.gene25436.t1 | MTR_2g038625 | 37.615 | 109 | 64 | 2 | 10 | 114 | 60 | 168 | 1.27e-11 | 62.4 |
| MS.gene25436.t1 | MTR_2g038580 | 37.615 | 109 | 64 | 2 | 10 | 114 | 60 | 168 | 1.27e-11 | 62.4 |
| MS.gene25436.t1 | MTR_5g013010 | 42.308 | 78 | 44 | 1 | 3 | 79 | 110 | 187 | 2.52e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25436.t1 | AT1G69780 | 67.364 | 239 | 60 | 5 | 6 | 233 | 63 | 294 | 2.95e-102 | 298 |
| MS.gene25436.t1 | AT1G26960 | 60.889 | 225 | 63 | 5 | 11 | 233 | 54 | 255 | 8.28e-86 | 255 |
| MS.gene25436.t1 | AT3G01220 | 53.425 | 219 | 78 | 6 | 15 | 226 | 72 | 273 | 6.50e-67 | 208 |
| MS.gene25436.t1 | AT5G15150 | 48.696 | 230 | 83 | 8 | 7 | 226 | 96 | 300 | 3.18e-59 | 189 |
| MS.gene25436.t1 | AT4G40060 | 57.576 | 132 | 52 | 2 | 10 | 137 | 39 | 170 | 1.14e-40 | 141 |
| MS.gene25436.t1 | AT5G65310 | 45.026 | 191 | 84 | 5 | 3 | 174 | 19 | 207 | 2.34e-40 | 140 |
| MS.gene25436.t1 | AT5G65310 | 45.026 | 191 | 84 | 5 | 3 | 174 | 37 | 225 | 2.94e-40 | 140 |
| MS.gene25436.t1 | AT2G22430 | 66.667 | 99 | 33 | 0 | 25 | 123 | 59 | 157 | 3.19e-39 | 137 |
| MS.gene25436.t1 | AT3G01470 | 65.979 | 97 | 33 | 0 | 24 | 120 | 64 | 160 | 4.32e-39 | 136 |
| MS.gene25436.t1 | AT1G27045 | 48.800 | 125 | 58 | 1 | 28 | 146 | 17 | 141 | 1.66e-28 | 106 |
| MS.gene25436.t1 | AT1G27045 | 48.800 | 125 | 58 | 1 | 28 | 146 | 25 | 149 | 1.71e-28 | 106 |
| MS.gene25436.t1 | AT1G27045 | 48.800 | 125 | 58 | 1 | 28 | 146 | 25 | 149 | 1.71e-28 | 106 |
| MS.gene25436.t1 | AT1G27045 | 45.517 | 145 | 71 | 2 | 10 | 146 | 48 | 192 | 2.10e-28 | 107 |
| MS.gene25436.t1 | AT2G46680 | 52.688 | 93 | 44 | 0 | 29 | 121 | 33 | 125 | 3.35e-28 | 107 |
| MS.gene25436.t1 | AT2G46680 | 53.333 | 90 | 42 | 0 | 29 | 118 | 33 | 122 | 1.75e-27 | 105 |
| MS.gene25436.t1 | AT1G27045 | 50.442 | 113 | 54 | 1 | 10 | 120 | 48 | 160 | 6.95e-27 | 104 |
| MS.gene25436.t1 | AT3G61890 | 51.111 | 90 | 44 | 0 | 31 | 120 | 33 | 122 | 5.92e-26 | 101 |
| MS.gene25436.t1 | AT5G03790 | 52.273 | 88 | 42 | 0 | 28 | 115 | 77 | 164 | 1.17e-24 | 97.8 |
| MS.gene25436.t1 | AT5G03790 | 55.000 | 80 | 36 | 0 | 28 | 107 | 77 | 156 | 7.99e-24 | 94.7 |
| MS.gene25436.t1 | AT2G36610 | 43.590 | 117 | 51 | 3 | 9 | 117 | 59 | 168 | 9.66e-23 | 91.7 |
| MS.gene25436.t1 | AT2G18550 | 46.341 | 123 | 56 | 3 | 28 | 147 | 61 | 176 | 1.12e-22 | 92.4 |
| MS.gene25436.t1 | AT4G36740 | 48.544 | 103 | 46 | 1 | 19 | 121 | 46 | 141 | 3.10e-22 | 91.3 |
| MS.gene25436.t1 | AT4G36740 | 48.544 | 103 | 46 | 1 | 19 | 121 | 47 | 142 | 3.20e-22 | 91.3 |
| MS.gene25436.t1 | AT5G66700 | 45.378 | 119 | 56 | 2 | 12 | 123 | 57 | 173 | 1.64e-21 | 89.4 |
| MS.gene25436.t1 | AT5G53980 | 44.554 | 101 | 56 | 0 | 23 | 123 | 6 | 106 | 3.71e-21 | 86.7 |
| MS.gene25436.t1 | AT2G01430 | 43.810 | 105 | 59 | 0 | 10 | 114 | 121 | 225 | 2.91e-18 | 81.6 |
| MS.gene25436.t1 | AT5G47370 | 42.708 | 96 | 55 | 0 | 20 | 115 | 122 | 217 | 2.70e-17 | 79.3 |
| MS.gene25436.t1 | AT4G37790 | 41.228 | 114 | 63 | 1 | 12 | 125 | 110 | 219 | 3.91e-17 | 78.6 |
| MS.gene25436.t1 | AT2G22800 | 46.667 | 90 | 48 | 0 | 26 | 115 | 111 | 200 | 5.40e-17 | 78.2 |
| MS.gene25436.t1 | AT5G06710 | 43.750 | 96 | 54 | 0 | 20 | 115 | 182 | 277 | 7.99e-17 | 78.6 |
| MS.gene25436.t1 | AT4G17460 | 43.000 | 100 | 55 | 1 | 15 | 114 | 124 | 221 | 1.14e-16 | 77.4 |
| MS.gene25436.t1 | AT3G60390 | 42.991 | 107 | 57 | 1 | 15 | 121 | 149 | 251 | 1.58e-16 | 77.4 |
| MS.gene25436.t1 | AT1G70920 | 37.903 | 124 | 69 | 2 | 8 | 131 | 50 | 165 | 3.63e-15 | 72.0 |
| MS.gene25436.t1 | AT1G70920 | 37.903 | 124 | 69 | 2 | 8 | 131 | 20 | 135 | 4.44e-15 | 71.2 |
| MS.gene25436.t1 | AT2G44910 | 44.681 | 94 | 48 | 1 | 28 | 121 | 163 | 252 | 5.16e-15 | 73.2 |
| MS.gene25436.t1 | AT4G16780 | 44.681 | 94 | 48 | 1 | 28 | 121 | 129 | 218 | 9.00e-15 | 72.0 |
| MS.gene25436.t1 | AT2G01430 | 50.704 | 71 | 35 | 0 | 10 | 80 | 121 | 191 | 1.31e-13 | 67.8 |
Find 39 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTTCTGTTTCTTTATTA+AGG | 0.139873 | 8.2:-40638990 | None:intergenic |
| CTGAATGTCTTGTCTTGAAT+TGG | 0.288821 | 8.2:-40639169 | None:intergenic |
| AAGACAATATGATACTATTA+AGG | 0.363112 | 8.2:+40638244 | MS.gene25436:CDS |
| AACCAAGACAGATTGCTATA+TGG | 0.383913 | 8.2:+40638159 | MS.gene25436:CDS |
| TCCGATGATGGATCGCAGTT+AGG | 0.391738 | 8.2:+40638020 | MS.gene25436:CDS |
| GTCTTGAAGAAGATGGAAAT+AGG | 0.399749 | 8.2:-40639116 | None:intergenic |
| CCGATGATGGATCGCAGTTA+GGG | 0.414857 | 8.2:+40638021 | MS.gene25436:CDS |
| ATGCAGCTAGCAAGGGCTCT+TGG | 0.429987 | 8.2:+40638131 | MS.gene25436:CDS |
| TGGATGATCAATCTGGACTA+TGG | 0.444127 | 8.2:+40639250 | MS.gene25436:CDS |
| CCATCTTCTTCAAGACCAGC+AGG | 0.460199 | 8.2:+40639123 | MS.gene25436:CDS |
| CTTGTCTGTTGAGTAGATAA+AGG | 0.476490 | 8.2:-40639087 | None:intergenic |
| AGCTGATGAGGAACTCCTGC+TGG | 0.491266 | 8.2:-40639138 | None:intergenic |
| TGTGCCATGGATGATCAATC+TGG | 0.494499 | 8.2:+40639243 | MS.gene25436:CDS |
| CCCTAACTGCGATCCATCAT+CGG | 0.502143 | 8.2:-40638021 | None:intergenic |
| CTATATGGTTTCAAAACAGA+AGG | 0.506981 | 8.2:+40638174 | MS.gene25436:CDS |
| CCTGCTGGTCTTGAAGAAGA+TGG | 0.521993 | 8.2:-40639123 | None:intergenic |
| ATCTGGACTATGGCCATGGT+TGG | 0.522424 | 8.2:+40639260 | MS.gene25436:CDS |
| AACCATATAGCAATCTGTCT+TGG | 0.526039 | 8.2:-40638161 | None:intergenic |
| AGAGGAAAATGCAGCTAGCA+AGG | 0.526858 | 8.2:+40638123 | MS.gene25436:CDS |
| TTAAGGTTGATTGATTCAGT+TGG | 0.542418 | 8.2:-40638973 | None:intergenic |
| GAGGAAGAATTATCCGATGA+TGG | 0.563562 | 8.2:+40638008 | MS.gene25436:CDS |
| GGTTTCAAAACAGAAGGGCT+AGG | 0.568856 | 8.2:+40638180 | MS.gene25436:CDS |
| TAGTCCAGATTGATCATCCA+TGG | 0.576768 | 8.2:-40639247 | None:intergenic |
| GTGTCAAAAGATTGATCACA+TGG | 0.589732 | 8.2:+40639191 | MS.gene25436:CDS |
| TTGGTTTGAAAGAGCTGATG+AGG | 0.592238 | 8.2:-40639150 | None:intergenic |
| AACCTTAATAAAGAAACAGA+AGG | 0.601474 | 8.2:+40638988 | MS.gene25436:CDS |
| GAAGAAGAGAAGGCTTAACA+TGG | 0.612268 | 8.2:+40638046 | MS.gene25436:CDS |
| ATCAATCTGGACTATGGCCA+TGG | 0.615676 | 8.2:+40639256 | MS.gene25436:CDS |
| AGTTAGGGGAGAAGAAGAGA+AGG | 0.616244 | 8.2:+40638036 | MS.gene25436:CDS |
| AAACAGTTCAGATATCAAGC+TGG | 0.622724 | 8.2:+40639032 | MS.gene25436:CDS |
| TAGGTGGAAGACAAAACAGT+TGG | 0.622940 | 8.2:+40638199 | MS.gene25436:CDS |
| TATATGGTTTCAAAACAGAA+GGG | 0.626685 | 8.2:+40638175 | MS.gene25436:CDS |
| GAGGAAAATGCAGCTAGCAA+GGG | 0.631443 | 8.2:+40638124 | MS.gene25436:CDS |
| AAGTGCTGTTGCTCCAACCA+TGG | 0.633621 | 8.2:-40639273 | None:intergenic |
| CGATGATGGATCGCAGTTAG+GGG | 0.649440 | 8.2:+40638022 | MS.gene25436:CDS |
| GAAACAAGCTTGAACCTGAG+AGG | 0.652955 | 8.2:+40638105 | MS.gene25436:CDS |
| TGGAGAAGAAGCTAATGTCG+AGG | 0.659865 | 8.2:+40637989 | MS.gene25436:CDS |
| TTCAAAACAGAAGGGCTAGG+TGG | 0.663747 | 8.2:+40638183 | MS.gene25436:CDS |
| TCAAGCTGGATATTTCAACA+AGG | 0.727961 | 8.2:+40639046 | MS.gene25436:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTCTTTTTATATTCAAA+AGG | - | chr8.2:40638662-40638681 | None:intergenic | 10.0% |
| !! | TTATAAAAGTTATCATTAGT+TGG | - | chr8.2:40638727-40638746 | None:intergenic | 15.0% |
| !!! | AAAAAAAAGCTTTTTACTTA+TGG | + | chr8.2:40638880-40638899 | MS.gene25436:intron | 15.0% |
| !!! | TTAATTTTTTTACCAAAATG+AGG | - | chr8.2:40638819-40638838 | None:intergenic | 15.0% |
| !! | AAATTGATGAAAGGAAATAA+AGG | - | chr8.2:40638702-40638721 | None:intergenic | 20.0% |
| !! | AAGACAATATGATACTATTA+AGG | + | chr8.2:40638244-40638263 | MS.gene25436:CDS | 20.0% |
| !! | CATTGAAAATTAGTAACATT+TGG | - | chr8.2:40638919-40638938 | None:intergenic | 20.0% |
| !! | GACACATAAATTAACAAATA+TGG | - | chr8.2:40638486-40638505 | None:intergenic | 20.0% |
| !!! | AACTTTTTATTCTTTACTCA+TGG | + | chr8.2:40638325-40638344 | MS.gene25436:intron | 20.0% |
| ! | AACCTTAATAAAGAAACAGA+AGG | + | chr8.2:40638988-40639007 | MS.gene25436:CDS | 25.0% |
| ! | TATATGGTTTCAAAACAGAA+GGG | + | chr8.2:40638175-40638194 | MS.gene25436:CDS | 25.0% |
| !! | ATCCTTCTGTTTCTTTATTA+AGG | - | chr8.2:40638993-40639012 | None:intergenic | 25.0% |
| !!! | CTTTTAGAACAACATTAGAT+TGG | - | chr8.2:40638414-40638433 | None:intergenic | 25.0% |
| !!! | GTAAAAAGCTTTTTTTTGCT+TGG | - | chr8.2:40638877-40638896 | None:intergenic | 25.0% |
| AAAGAAAACATGTCATTGAG+TGG | - | chr8.2:40638786-40638805 | None:intergenic | 30.0% | |
| CTATATGGTTTCAAAACAGA+AGG | + | chr8.2:40638174-40638193 | MS.gene25436:CDS | 30.0% | |
| GAAAGTAACTGTGTAATGAT+AGG | - | chr8.2:40638764-40638783 | None:intergenic | 30.0% | |
| ! | ATGACATGTTTTCTTTTGCA+TGG | + | chr8.2:40638790-40638809 | MS.gene25436:intron | 30.0% |
| ! | CAAGAGATGTTCAAGTATTT+TGG | + | chr8.2:40638541-40638560 | MS.gene25436:intron | 30.0% |
| ! | TTAAGGTTGATTGATTCAGT+TGG | - | chr8.2:40638976-40638995 | None:intergenic | 30.0% |
| !! | TAGTTGGTGAAATTGATGAA+AGG | - | chr8.2:40638711-40638730 | None:intergenic | 30.0% |
| AAACAGTTCAGATATCAAGC+TGG | + | chr8.2:40639032-40639051 | MS.gene25436:CDS | 35.0% | |
| AACCAAGACAGATTGCTATA+TGG | + | chr8.2:40638159-40638178 | MS.gene25436:CDS | 35.0% | |
| AACCATATAGCAATCTGTCT+TGG | - | chr8.2:40638164-40638183 | None:intergenic | 35.0% | |
| CTGAATGTCTTGTCTTGAAT+TGG | - | chr8.2:40639172-40639191 | None:intergenic | 35.0% | |
| CTTGTCTGTTGAGTAGATAA+AGG | - | chr8.2:40639090-40639109 | None:intergenic | 35.0% | |
| GAATCAAAAACTTCAGACTG+AGG | + | chr8.2:40638292-40638311 | MS.gene25436:CDS | 35.0% | |
| GTCTTGAAGAAGATGGAAAT+AGG | - | chr8.2:40639119-40639138 | None:intergenic | 35.0% | |
| GTGTCAAAAGATTGATCACA+TGG | + | chr8.2:40639191-40639210 | MS.gene25436:CDS | 35.0% | |
| TCAAGCTGGATATTTCAACA+AGG | + | chr8.2:40639046-40639065 | MS.gene25436:CDS | 35.0% | |
| ! | TCAATGCTTGTTGCAGATAT+TGG | + | chr8.2:40638933-40638952 | MS.gene25436:intron | 35.0% |
| ! | TTTGCATGGTTTCCTCATTT+TGG | + | chr8.2:40638804-40638823 | MS.gene25436:intron | 35.0% |
| !! | TCATTTTCAGGGATAGAACT+TGG | + | chr8.2:40637969-40637988 | MS.gene25436:CDS | 35.0% |
| !!! | CTTGAGAAGAGTTTTGAGTT+AGG | + | chr8.2:40638083-40638102 | MS.gene25436:CDS | 35.0% |
| CAACAAGAGCTGAATTTCAG+TGG | + | chr8.2:40638377-40638396 | MS.gene25436:intron | 40.0% | |
| GAAGAAGAGAAGGCTTAACA+TGG | + | chr8.2:40638046-40638065 | MS.gene25436:CDS | 40.0% | |
| GAGGAAGAATTATCCGATGA+TGG | + | chr8.2:40638008-40638027 | MS.gene25436:CDS | 40.0% | |
| TAGGTGGAAGACAAAACAGT+TGG | + | chr8.2:40638199-40638218 | MS.gene25436:CDS | 40.0% | |
| TAGTCCAGATTGATCATCCA+TGG | - | chr8.2:40639250-40639269 | None:intergenic | 40.0% | |
| TGGATGATCAATCTGGACTA+TGG | + | chr8.2:40639250-40639269 | MS.gene25436:CDS | 40.0% | |
| ! | GAGTAACATGTTTTGTGCCA+TGG | + | chr8.2:40639230-40639249 | MS.gene25436:CDS | 40.0% |
| ! | TTGGTTTGAAAGAGCTGATG+AGG | - | chr8.2:40639153-40639172 | None:intergenic | 40.0% |
| AGAGGAAAATGCAGCTAGCA+AGG | + | chr8.2:40638123-40638142 | MS.gene25436:CDS | 45.0% | |
| AGTTAGGGGAGAAGAAGAGA+AGG | + | chr8.2:40638036-40638055 | MS.gene25436:CDS | 45.0% | |
| ATCAATCTGGACTATGGCCA+TGG | + | chr8.2:40639256-40639275 | MS.gene25436:CDS | 45.0% | |
| GAAACAAGCTTGAACCTGAG+AGG | + | chr8.2:40638105-40638124 | MS.gene25436:CDS | 45.0% | |
| GAGGAAAATGCAGCTAGCAA+GGG | + | chr8.2:40638124-40638143 | MS.gene25436:CDS | 45.0% | |
| GGTTTCAAAACAGAAGGGCT+AGG | + | chr8.2:40638180-40638199 | MS.gene25436:CDS | 45.0% | |
| TGGAGAAGAAGCTAATGTCG+AGG | + | chr8.2:40637989-40638008 | MS.gene25436:CDS | 45.0% | |
| TGTGCCATGGATGATCAATC+TGG | + | chr8.2:40639243-40639262 | MS.gene25436:CDS | 45.0% | |
| TTCAAAACAGAAGGGCTAGG+TGG | + | chr8.2:40638183-40638202 | MS.gene25436:CDS | 45.0% | |
| ATCTGGACTATGGCCATGGT+TGG | + | chr8.2:40639260-40639279 | MS.gene25436:CDS | 50.0% | |
| CCATCTTCTTCAAGACCAGC+AGG | + | chr8.2:40639123-40639142 | MS.gene25436:CDS | 50.0% | |
| CCCTAACTGCGATCCATCAT+CGG | - | chr8.2:40638024-40638043 | None:intergenic | 50.0% | |
| ! | AAGTGCTGTTGCTCCAACCA+TGG | - | chr8.2:40639276-40639295 | None:intergenic | 50.0% |
| ! | GCTAGCTGCATTTTCCTCTC+AGG | - | chr8.2:40638122-40638141 | None:intergenic | 50.0% |
| !! | CCGATGATGGATCGCAGTTA+GGG | + | chr8.2:40638021-40638040 | MS.gene25436:CDS | 50.0% |
| !! | CCTGCTGGTCTTGAAGAAGA+TGG | - | chr8.2:40639126-40639145 | None:intergenic | 50.0% |
| !! | CGATGATGGATCGCAGTTAG+GGG | + | chr8.2:40638022-40638041 | MS.gene25436:CDS | 50.0% |
| !! | TCCGATGATGGATCGCAGTT+AGG | + | chr8.2:40638020-40638039 | MS.gene25436:CDS | 50.0% |
| AGCTGATGAGGAACTCCTGC+TGG | - | chr8.2:40639141-40639160 | None:intergenic | 55.0% | |
| ATGCAGCTAGCAAGGGCTCT+TGG | + | chr8.2:40638131-40638150 | MS.gene25436:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 40637966 | 40639302 | 40637966 | ID=MS.gene25436 |
| chr8.2 | mRNA | 40637966 | 40639302 | 40637966 | ID=MS.gene25436.t1;Parent=MS.gene25436 |
| chr8.2 | exon | 40637966 | 40638313 | 40637966 | ID=MS.gene25436.t1.exon1;Parent=MS.gene25436.t1 |
| chr8.2 | CDS | 40637966 | 40638313 | 40637966 | ID=cds.MS.gene25436.t1;Parent=MS.gene25436.t1 |
| chr8.2 | exon | 40638949 | 40639302 | 40638949 | ID=MS.gene25436.t1.exon2;Parent=MS.gene25436.t1 |
| chr8.2 | CDS | 40638949 | 40639302 | 40638949 | ID=cds.MS.gene25436.t1;Parent=MS.gene25436.t1 |
| Gene Sequence |
| Protein sequence |
