AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068257.t1 MTR_4g012940 100.000 207 0 0 1 207 1 207 4.48e-157 432
MS.gene068257.t1 MTR_2g101890 96.098 205 8 0 3 207 5 209 6.54e-151 417
MS.gene068257.t1 MTR_4g069850 88.406 207 23 1 1 207 1 206 8.41e-137 381
MS.gene068257.t1 MTR_1g097420 72.464 207 57 0 1 207 1 207 8.98e-111 315
MS.gene068257.t1 MTR_1g097420 64.103 234 57 1 1 207 1 234 1.19e-104 301
MS.gene068257.t1 MTR_8g092020 64.706 204 72 0 2 205 6 209 2.98e-100 289
MS.gene068257.t1 MTR_6g039490 64.865 185 65 0 1 185 1 185 1.51e-85 252
MS.gene068257.t1 MTR_1452s0010 81.928 83 15 0 28 110 1 83 1.14e-46 149
MS.gene068257.t1 MTR_5g013900 39.216 204 111 3 10 207 11 207 2.55e-42 142
MS.gene068257.t1 MTR_2g005510 36.607 224 118 6 1 207 1 217 4.12e-41 139
MS.gene068257.t1 MTR_2g005500 36.318 201 118 4 8 205 9 202 1.39e-38 132
MS.gene068257.t1 MTR_4g098950 37.438 203 115 3 10 207 11 206 1.96e-38 132
MS.gene068257.t1 MTR_8g006970 37.313 201 116 4 8 205 22 215 2.16e-38 132
MS.gene068257.t1 MTR_4g064897 35.885 209 116 5 8 205 12 213 2.92e-38 132
MS.gene068257.t1 MTR_3g087160 38.235 204 112 4 10 207 11 206 3.59e-38 131
MS.gene068257.t1 MTR_8g090215 39.216 204 111 4 10 207 11 207 3.86e-38 131
MS.gene068257.t1 MTR_7g081700 37.811 201 114 5 8 205 8 200 6.70e-38 130
MS.gene068257.t1 MTR_2g014990 38.500 200 101 7 8 195 13 202 8.39e-38 130
MS.gene068257.t1 MTR_4g088090 37.500 200 103 7 8 195 13 202 2.54e-37 129
MS.gene068257.t1 MTR_3g069510 36.667 210 114 7 8 205 12 214 3.07e-37 129
MS.gene068257.t1 MTR_1g068740 37.379 206 117 5 8 207 15 214 4.48e-37 129
MS.gene068257.t1 MTR_2g096550 38.983 177 103 4 8 184 15 186 5.41e-37 128
MS.gene068257.t1 MTR_4g079350 40.741 162 89 3 8 169 13 167 8.97e-37 128
MS.gene068257.t1 MTR_1g068730 36.893 206 118 5 8 207 15 214 1.29e-36 127
MS.gene068257.t1 MTR_1g115325 35.122 205 120 4 8 205 15 213 1.85e-36 127
MS.gene068257.t1 MTR_8g006970 40.964 166 91 3 8 173 8 166 3.60e-36 125
MS.gene068257.t1 MTR_7g100020 40.476 168 94 3 8 175 15 176 3.90e-36 126
MS.gene068257.t1 MTR_1686s0010 65.000 80 28 0 28 107 1 80 4.60e-35 119
MS.gene068257.t1 MTR_2g075950 33.971 209 120 5 8 205 13 214 6.91e-35 123
MS.gene068257.t1 MTR_8g036220 40.741 162 89 3 8 169 16 170 1.04e-34 123
MS.gene068257.t1 MTR_1g052475 36.508 189 113 3 8 196 15 196 3.12e-34 121
MS.gene068257.t1 MTR_1663s0010 70.667 75 22 0 110 184 1 75 5.23e-34 116
MS.gene068257.t1 MTR_7g405790 36.628 172 102 3 8 179 15 179 7.04e-34 120
MS.gene068257.t1 MTR_2g090865 39.506 162 91 3 8 169 11 165 1.23e-33 120
MS.gene068257.t1 MTR_6g021790 37.791 172 100 3 8 179 6 170 2.28e-33 119
MS.gene068257.t1 MTR_8g103550 35.714 182 110 3 3 184 4 178 2.96e-33 118
MS.gene068257.t1 MTR_7g051940 36.047 172 103 3 8 179 13 177 4.18e-33 118
MS.gene068257.t1 MTR_4g063160 36.232 207 117 6 8 205 15 215 4.48e-33 118
MS.gene068257.t1 MTR_3g086980 37.576 165 96 3 8 172 16 173 8.91e-33 117
MS.gene068257.t1 MTR_2g083860 38.272 162 93 3 8 169 13 167 2.56e-32 116
MS.gene068257.t1 MTR_8g089985 36.095 169 101 3 4 172 13 174 3.62e-32 116
MS.gene068257.t1 MTR_8g031680 36.702 188 109 4 9 196 7 184 3.66e-32 115
MS.gene068257.t1 MTR_0262s0050 38.788 165 94 3 8 172 15 172 6.06e-32 115
MS.gene068257.t1 MTR_3g064390 35.632 174 105 3 10 183 12 178 1.73e-31 114
MS.gene068257.t1 MTR_1g078200 37.126 167 98 4 8 174 14 173 2.70e-31 114
MS.gene068257.t1 MTR_5g076590 35.632 174 105 3 10 183 12 178 3.13e-31 113
MS.gene068257.t1 MTR_6g079550 37.654 162 94 3 8 169 12 166 4.73e-31 113
MS.gene068257.t1 MTR_2g027660 37.654 162 94 3 8 169 6 160 5.18e-31 113
MS.gene068257.t1 MTR_4g099470 38.068 176 98 5 33 205 9 176 6.78e-31 111
MS.gene068257.t1 MTR_8g027220 33.032 221 127 6 1 207 19 232 6.70e-30 110
MS.gene068257.t1 MTR_8g107070 38.272 162 93 3 8 169 14 168 1.34e-29 110
MS.gene068257.t1 MTR_8g031870 35.758 165 99 3 5 169 3 160 2.17e-29 108
MS.gene068257.t1 MTR_1g094220 34.503 171 104 4 5 174 28 191 9.79e-29 106
MS.gene068257.t1 MTR_8g093740 36.842 171 100 5 8 177 13 176 9.87e-29 107
MS.gene068257.t1 MTR_8g031660 36.257 171 102 3 8 178 6 169 4.04e-28 105
MS.gene068257.t1 MTR_1g094220 33.146 178 111 4 5 181 28 198 4.21e-28 105
MS.gene068257.t1 MTR_1g068730 40.650 123 69 1 8 130 15 133 5.26e-28 103
MS.gene068257.t1 MTR_7g117910 37.162 148 85 3 8 155 20 159 8.99e-28 104
MS.gene068257.t1 MTR_1g068740 40.650 123 69 1 8 130 15 133 9.78e-28 104
MS.gene068257.t1 MTR_1812s0010 60.274 73 29 0 111 183 2 74 3.71e-27 99.0
MS.gene068257.t1 MTR_1g029450 33.663 202 108 6 8 190 17 211 5.24e-27 102
MS.gene068257.t1 MTR_1g094220 33.333 168 104 4 5 171 28 188 1.20e-26 101
MS.gene068257.t1 MTR_1g094220 33.533 167 103 4 5 170 28 187 1.43e-26 102
MS.gene068257.t1 MTR_8g031830 33.679 193 110 5 5 197 3 177 2.38e-26 100
MS.gene068257.t1 MTR_7g103070 36.970 165 97 5 10 173 18 176 2.42e-26 100
MS.gene068257.t1 MTR_2g102060 31.053 190 124 3 10 199 12 194 2.78e-26 100
MS.gene068257.t1 MTR_1g090717 37.805 164 94 5 9 170 16 173 2.17e-25 98.6
MS.gene068257.t1 MTR_3g096350 35.976 164 97 5 8 170 12 168 2.37e-25 98.2
MS.gene068257.t1 MTR_1g062760 36.364 165 97 5 10 173 16 173 4.75e-25 97.4
MS.gene068257.t1 MTR_2g093510 74.627 67 17 0 119 185 761 827 8.88e-25 101
MS.gene068257.t1 MTR_3g064390 38.017 121 71 1 10 130 12 128 1.92e-24 94.7
MS.gene068257.t1 MTR_3g107717 31.792 173 94 6 9 174 14 169 1.76e-22 91.3
MS.gene068257.t1 MTR_3g107713 31.792 173 94 6 9 174 14 169 2.01e-22 90.9
MS.gene068257.t1 MTR_3g107707 31.792 173 94 6 9 174 14 169 2.03e-22 90.9
MS.gene068257.t1 MTR_3g096350 38.525 122 70 3 8 128 12 129 6.47e-22 87.8
MS.gene068257.t1 MTR_3g096350 38.525 122 70 3 8 128 12 129 1.28e-21 87.0
MS.gene068257.t1 MTR_6g087980 30.769 208 120 7 8 206 6 198 1.19e-20 85.9
MS.gene068257.t1 MTR_8g093740 37.594 133 76 4 39 170 10 136 2.15e-20 84.3
MS.gene068257.t1 MTR_4g088055 30.108 186 113 6 1 177 1 178 8.83e-18 78.2
MS.gene068257.t1 MTR_5g036990 52.857 70 32 1 39 107 32 101 1.39e-17 75.5
MS.gene068257.t1 MTR_4g113620 29.480 173 99 6 9 179 14 165 2.23e-17 77.4
MS.gene068257.t1 MTR_2g090875 29.609 179 111 5 8 177 6 178 5.52e-17 75.9
MS.gene068257.t1 MTR_3g078260 30.481 187 115 5 1 178 1 181 1.22e-16 75.5
MS.gene068257.t1 MTR_5g022600 29.570 186 114 7 1 177 1 178 1.48e-16 74.7
MS.gene068257.t1 MTR_8g075240 30.108 186 113 7 1 177 1 178 3.47e-16 73.9
MS.gene068257.t1 MTR_7g103070 38.983 118 65 5 57 173 9 120 5.92e-16 72.4
MS.gene068257.t1 MTR_4g073250 27.419 186 120 4 8 184 6 185 1.20e-15 72.4
MS.gene068257.t1 MTR_3g078260 35.156 128 76 4 1 127 1 122 1.38e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068257.t1 AT1G52280 91.304 207 17 1 1 207 1 206 3.93e-142 395
MS.gene068257.t1 AT3G16100 90.821 207 18 1 1 207 1 206 1.42e-141 393
MS.gene068257.t1 AT3G18820 89.855 207 20 1 1 207 1 206 6.00e-138 384
MS.gene068257.t1 AT1G49300 87.440 207 25 1 1 207 1 206 8.45e-134 374
MS.gene068257.t1 AT1G49300 87.440 207 25 1 1 207 1 206 8.45e-134 374
MS.gene068257.t1 AT4G09720 70.952 210 54 2 1 207 1 206 4.35e-109 311
MS.gene068257.t1 AT1G22740 71.635 208 53 2 1 207 1 203 6.93e-109 311
MS.gene068257.t1 AT4G09720 67.421 221 54 3 1 207 1 217 1.83e-105 302
MS.gene068257.t1 AT4G09720 68.837 215 55 3 1 207 1 211 2.04e-105 302
MS.gene068257.t1 AT2G21880 63.551 214 62 3 4 207 5 212 2.55e-94 274
MS.gene068257.t1 AT4G09720 68.571 175 48 2 36 207 2 172 1.65e-85 250
MS.gene068257.t1 AT4G09720 68.571 175 48 2 36 207 2 172 1.65e-85 250
MS.gene068257.t1 AT4G09720 68.571 175 48 2 36 207 2 172 1.65e-85 250
MS.gene068257.t1 AT2G21880 59.813 214 62 4 4 207 5 204 1.40e-84 249
MS.gene068257.t1 AT2G21880 61.658 193 58 3 25 207 1 187 1.06e-80 239
MS.gene068257.t1 AT2G21880 61.658 193 58 3 25 207 1 187 1.06e-80 239
MS.gene068257.t1 AT5G39620 58.140 172 72 0 9 180 6 177 5.20e-71 215
MS.gene068257.t1 AT2G44610 39.512 205 110 4 10 207 11 208 5.08e-41 138
MS.gene068257.t1 AT1G28550 37.143 210 113 5 8 205 13 215 3.00e-39 134
MS.gene068257.t1 AT1G09630 41.818 165 89 3 8 172 12 169 3.54e-39 134
MS.gene068257.t1 AT5G45750 36.364 209 114 6 8 205 13 213 8.93e-38 130
MS.gene068257.t1 AT2G33870 35.238 210 117 5 8 205 13 215 1.45e-37 130
MS.gene068257.t1 AT5G60860 38.384 198 104 6 8 195 13 202 1.70e-37 130
MS.gene068257.t1 AT3G15060 41.358 162 88 3 8 169 13 167 3.34e-37 129
MS.gene068257.t1 AT4G17530 39.548 177 97 4 8 181 8 177 3.39e-37 129
MS.gene068257.t1 AT5G59840 37.379 206 115 5 8 205 15 214 3.95e-37 129
MS.gene068257.t1 AT5G47200 37.811 201 113 5 8 205 8 199 6.01e-37 128
MS.gene068257.t1 AT5G64990 39.810 211 101 8 10 207 9 206 6.70e-37 128
MS.gene068257.t1 AT4G18430 34.762 210 117 7 8 205 13 214 1.73e-36 127
MS.gene068257.t1 AT3G53610 38.350 206 113 7 8 205 15 214 2.21e-36 127
MS.gene068257.t1 AT3G53610 38.350 206 113 7 8 205 15 214 2.21e-36 127
MS.gene068257.t1 AT3G53610 38.350 206 113 7 8 205 15 214 2.21e-36 127
MS.gene068257.t1 AT1G02130 35.821 201 119 4 8 205 8 201 2.45e-36 126
MS.gene068257.t1 AT5G10260 37.561 205 113 4 10 207 11 207 3.86e-36 126
MS.gene068257.t1 AT5G59150 38.182 165 95 3 8 172 12 169 4.98e-36 126
MS.gene068257.t1 AT3G46060 36.408 206 117 5 8 205 15 214 7.54e-36 125
MS.gene068257.t1 AT3G46060 36.408 206 117 5 8 205 15 214 7.54e-36 125
MS.gene068257.t1 AT3G46060 36.408 206 117 5 8 205 15 214 7.54e-36 125
MS.gene068257.t1 AT3G11730 35.407 209 124 4 1 205 1 202 7.94e-36 125
MS.gene068257.t1 AT1G07410 38.182 165 95 3 8 172 12 169 1.24e-35 125
MS.gene068257.t1 AT1G05810 35.714 182 110 3 4 185 8 182 1.32e-35 125
MS.gene068257.t1 AT3G46830 38.788 165 94 3 8 172 12 169 1.48e-35 125
MS.gene068257.t1 AT3G46830 38.788 165 94 3 8 172 12 169 1.48e-35 125
MS.gene068257.t1 AT1G05810 35.714 182 110 3 4 185 54 228 4.01e-35 125
MS.gene068257.t1 AT3G12160 40.606 165 91 3 8 172 15 172 7.32e-35 123
MS.gene068257.t1 AT4G39890 40.000 165 93 2 9 173 10 168 1.15e-34 122
MS.gene068257.t1 AT2G31680 34.831 178 109 3 8 185 12 182 1.78e-34 122
MS.gene068257.t1 AT5G64990 38.532 218 101 9 10 207 9 213 3.88e-34 121
MS.gene068257.t1 AT1G06400 34.928 209 117 6 8 205 13 213 3.93e-34 121
MS.gene068257.t1 AT4G18800 39.506 162 91 3 8 169 13 167 5.05e-34 120
MS.gene068257.t1 AT5G03520 41.667 168 92 3 8 175 15 176 7.82e-34 120
MS.gene068257.t1 AT2G22290 36.408 206 114 4 10 207 11 207 9.21e-34 120
MS.gene068257.t1 AT4G35860 37.791 172 100 3 8 179 6 170 1.47e-33 119
MS.gene068257.t1 AT5G47960 36.757 185 101 4 8 183 15 192 1.90e-33 119
MS.gene068257.t1 AT1G16920 38.889 162 92 4 8 169 13 167 2.47e-33 119
MS.gene068257.t1 AT4G39990 37.576 165 96 3 8 172 17 174 3.05e-33 119
MS.gene068257.t1 AT2G43130 34.831 178 109 3 8 185 12 182 3.27e-33 119
MS.gene068257.t1 AT4G19640 36.612 183 106 4 10 189 12 187 9.08e-33 117
MS.gene068257.t1 AT1G18200 40.123 162 90 3 8 169 13 167 9.36e-33 118
MS.gene068257.t1 AT3G09900 42.262 168 91 4 8 175 15 176 9.50e-33 117
MS.gene068257.t1 AT5G45130 35.678 199 114 5 10 205 12 199 9.58e-33 117
MS.gene068257.t1 AT4G17160 34.314 204 120 4 9 205 7 203 2.78e-32 116
MS.gene068257.t1 AT5G65270 36.364 165 98 3 8 172 17 174 4.82e-32 116
MS.gene068257.t1 AT3G07410 35.465 172 104 3 8 179 12 176 7.89e-32 115
MS.gene068257.t1 AT5G45130 35.533 197 113 5 12 205 22 207 9.25e-32 115
MS.gene068257.t1 AT1G73640 38.889 162 92 3 8 169 13 167 2.96e-31 114
MS.gene068257.t1 AT4G17170 37.654 162 94 3 8 169 6 160 5.66e-31 113
MS.gene068257.t1 AT5G47520 33.673 196 118 5 8 198 14 202 6.95e-31 113
MS.gene068257.t1 AT1G01200 36.420 162 97 2 8 169 28 183 1.45e-29 110
MS.gene068257.t1 AT3G54840 34.503 171 104 4 2 171 27 190 1.64e-29 108
MS.gene068257.t1 AT3G54840 33.702 181 112 4 2 181 27 200 1.74e-29 108
MS.gene068257.t1 AT3G54840 34.302 172 105 4 11 181 2 166 1.65e-28 105
MS.gene068257.t1 AT1G43890 36.416 173 102 5 8 179 13 178 2.45e-27 103
MS.gene068257.t1 AT1G43890 36.416 173 102 5 8 179 13 178 2.45e-27 103
MS.gene068257.t1 AT1G43890 36.416 173 102 5 8 179 13 178 2.45e-27 103
MS.gene068257.t1 AT5G03530 35.052 194 100 7 10 184 15 201 1.95e-26 101
MS.gene068257.t1 AT5G03530 35.052 194 100 7 10 184 15 201 1.95e-26 101
MS.gene068257.t1 AT4G19640 37.226 137 81 2 10 146 12 143 6.69e-26 98.2
MS.gene068257.t1 AT5G03520 42.424 132 70 3 44 175 41 166 2.18e-25 98.6
MS.gene068257.t1 AT4G19640 39.167 120 69 1 10 129 12 127 1.32e-24 95.9
MS.gene068257.t1 AT5G20020 31.792 173 94 6 9 174 14 169 2.04e-22 90.9
MS.gene068257.t1 AT5G55190 31.792 173 94 6 9 174 14 169 2.09e-22 90.9
MS.gene068257.t1 AT5G20010 31.792 173 94 6 9 174 14 169 2.13e-22 90.9
MS.gene068257.t1 AT5G59150 37.398 123 70 3 50 172 3 118 1.65e-21 87.0
MS.gene068257.t1 AT3G09910 34.302 172 105 5 9 179 14 178 3.39e-21 87.0
MS.gene068257.t1 AT3G09910 34.302 172 105 5 9 179 14 178 3.39e-21 87.0
MS.gene068257.t1 AT5G55080 28.916 166 108 3 9 174 14 169 6.06e-21 87.0
MS.gene068257.t1 AT4G35860 36.923 130 75 3 50 179 2 124 1.11e-20 85.1
MS.gene068257.t1 AT3G09910 34.337 166 101 5 9 173 14 172 1.53e-20 85.5
MS.gene068257.t1 AT3G09910 34.337 166 101 5 9 173 14 172 1.53e-20 85.5
MS.gene068257.t1 AT3G09910 34.337 166 101 5 9 173 14 172 1.53e-20 85.5
MS.gene068257.t1 AT4G28950 30.583 206 122 7 8 206 6 197 2.35e-20 85.1
MS.gene068257.t1 AT5G62880 31.551 187 113 5 1 178 1 181 1.15e-17 78.2
MS.gene068257.t1 AT3G48040 31.551 187 113 5 1 178 1 181 1.86e-17 77.4
MS.gene068257.t1 AT5G45970 28.889 180 113 5 8 178 6 179 4.86e-17 76.3
MS.gene068257.t1 AT1G20090 29.570 186 113 6 1 177 1 177 1.36e-16 75.1
MS.gene068257.t1 AT4G35020 28.877 187 116 5 1 178 1 179 5.90e-16 73.2
MS.gene068257.t1 AT4G35020 28.877 187 116 5 1 178 1 179 5.90e-16 73.2
MS.gene068257.t1 AT4G35020 28.877 187 116 5 1 178 1 179 5.90e-16 73.2
MS.gene068257.t1 AT5G62880 31.667 180 108 5 1 171 1 174 1.06e-15 72.4
MS.gene068257.t1 AT3G51300 29.032 186 115 6 1 177 1 178 1.53e-15 72.0
MS.gene068257.t1 AT1G75840 28.495 186 116 6 1 177 1 178 1.54e-15 72.0
MS.gene068257.t1 AT2G44690 29.240 171 106 5 8 169 18 182 2.20e-15 72.0
MS.gene068257.t1 AT3G48040 35.385 130 77 4 1 129 1 124 3.67e-15 70.9
MS.gene068257.t1 AT2G17800 28.495 186 116 6 1 177 1 178 8.07e-15 70.1
MS.gene068257.t1 AT2G17800 28.495 186 116 6 1 177 1 178 8.07e-15 70.1
MS.gene068257.t1 AT4G35950 28.495 186 116 6 1 177 1 178 1.10e-14 70.1
MS.gene068257.t1 AT5G09910 25.510 196 116 5 9 176 23 216 3.32e-14 70.5
MS.gene068257.t1 AT5G09910 25.510 196 116 5 9 176 23 216 3.32e-14 70.5
MS.gene068257.t1 AT4G35020 32.031 128 78 5 1 127 1 120 2.34e-12 62.4
MS.gene068257.t1 AT4G35020 32.031 128 78 5 1 127 1 120 2.34e-12 62.4

Find 45 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTCCCATTTGTTGTATT+GGG 0.249578 4.4:-87331951 MS.gene068257:CDS
AAAACTCACATTTCTTCTTC+AGG 0.276765 4.4:+87331637 None:intergenic
AAACTTCCCATTTGTTGTAT+TGG 0.287627 4.4:-87331952 MS.gene068257:CDS
GAGAGAAGAATTTCTCATTC+AGG 0.301835 4.4:-87332097 MS.gene068257:intron
TTTGCGGAGGTCTCAAAGTA+TGG 0.329676 4.4:+87331721 None:intergenic
TTGTTTCCCAATACAACAAA+TGG 0.354677 4.4:+87331945 None:intergenic
ATTCTTCTCTCCAATGGTTA+AGG 0.368122 4.4:+87332109 None:intergenic
AGACAGGTTGTTCACATTGC+AGG 0.380730 4.4:-87333483 MS.gene068257:intron
GTCTTGGTGTGGCTTTCTAC+CGG 0.383425 4.4:-87332188 MS.gene068257:CDS
GTCTTCAAATTGAACTTCCT+TGG 0.388732 4.4:+87333502 None:intergenic
GTCATTATCCTCGGTGATAG+TGG 0.400216 4.4:-87334131 MS.gene068257:CDS
TCTTGGTGTGGCTTTCTACC+GGG 0.439363 4.4:-87332187 MS.gene068257:CDS
AAAGGCATGGTGTGCTTCAA+AGG 0.441249 4.4:-87331752 MS.gene068257:CDS
CTTTCGAGAACCTTAACCAT+TGG 0.448271 4.4:-87332119 MS.gene068257:CDS
AGGAAGTTCAATTTGAAGAC+AGG 0.464928 4.4:-87333499 MS.gene068257:CDS
CAAGAGCGGTTTCAGAGTCT+TGG 0.478744 4.4:-87332204 MS.gene068257:CDS
GGAAACAAAATAGACGTTGA+TGG 0.480216 4.4:-87331930 MS.gene068257:CDS
TTTGAGACCTCCGCAAAAGA+AGG 0.490684 4.4:-87331715 MS.gene068257:CDS
TGAGAAATTCTTCTCTCCAA+TGG 0.527269 4.4:+87332103 None:intergenic
TTGCCGACATCGATTGTGTC+GGG 0.530187 4.4:+87330908 None:intergenic
TTGCATTTAGGTATGTGAAT+CGG 0.536926 4.4:-87333571 MS.gene068257:intron
TGTTTCCCAATACAACAAAT+GGG 0.537587 4.4:+87331946 None:intergenic
ACAATCGATGTCGGCAATGG+TGG 0.539871 4.4:-87330902 MS.gene068257:CDS
AAGGCATGGTGTGCTTCAAA+GGG 0.542588 4.4:-87331751 MS.gene068257:CDS
TTGTTAAAAGTCATTATCCT+CGG 0.545319 4.4:-87334140 MS.gene068257:CDS
AACAAAATAGACGTTGATGG+TGG 0.573478 4.4:-87331927 MS.gene068257:CDS
GACACAATCGATGTCGGCAA+TGG 0.578998 4.4:-87330905 MS.gene068257:CDS
ACCCAGATTTGGGATACTGC+TGG 0.579202 4.4:-87332228 MS.gene068257:intron
AATCAGTACAAGGCTACCAT+TGG 0.582311 4.4:-87333539 MS.gene068257:CDS
CTTGGTGTGGCTTTCTACCG+GGG 0.583346 4.4:-87332186 MS.gene068257:CDS
TACTCAACATTCACAGCCTG+TGG 0.585248 4.4:+87330865 None:intergenic
ACAAAATAGACGTTGATGGT+GGG 0.586912 4.4:-87331926 MS.gene068257:CDS
GCGGTTTCAGAGTCTTGGTG+TGG 0.587655 4.4:-87332199 MS.gene068257:CDS
TCATTATCCTCGGTGATAGT+GGG 0.588594 4.4:-87334130 MS.gene068257:intron
GGACGACAACAGAGATCCAC+AGG 0.593912 4.4:-87330881 MS.gene068257:CDS
GGTGAGAAAATCAGCACCAA+TGG 0.597199 4.4:+87333523 None:intergenic
CTGAGAAGAAAGCAAAGGCA+TGG 0.611252 4.4:-87331765 MS.gene068257:CDS
GGGATACTGCTGGTCAAGAG+CGG 0.626594 4.4:-87332218 MS.gene068257:CDS
GAAGTTTAGTAATCAGTACA+AGG 0.630236 4.4:-87333549 MS.gene068257:CDS
CTGCCCGACACAATCGATGT+CGG 0.645647 4.4:-87330911 MS.gene068257:CDS
ATTGCCGACATCGATTGTGT+CGG 0.650688 4.4:+87330907 None:intergenic
GGTTTCTGAGAAGAAAGCAA+AGG 0.669924 4.4:-87331770 MS.gene068257:CDS
TGACATTTACATCATAAACA+AGG 0.673979 4.4:+87332148 None:intergenic
GGACGCAGCAATCAGCACCC+CGG 0.684255 4.4:+87332169 None:intergenic
CAGCATACCCACTATCACCG+AGG 0.788599 4.4:+87334123 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGAAAATTAATGAATA+AGG + chr4.4:87333629-87333648 None:intergenic 10.0%
!! AAGGTTATAAAATTAAAATT+TGG - chr4.4:87332511-87332530 MS.gene068257:intron 10.0%
!! TATAAAATTAAAATTTGGAA+AGG - chr4.4:87332516-87332535 MS.gene068257:intron 10.0%
!! CAAAATTAACCAAAAAAAAA+AGG + chr4.4:87333826-87333845 None:intergenic 15.0%
!! TCAAATTTAACAAAATTTGA+TGG - chr4.4:87331067-87331086 MS.gene068257:intron 15.0%
!! TTCTTAAATAAAAAGAGATA+TGG + chr4.4:87332998-87333017 None:intergenic 15.0%
!!! AAAATCAATGAATGATTTTA+AGG - chr4.4:87333522-87333541 MS.gene068257:CDS 15.0%
!!! AAATCAATGAATGATTTTAA+GGG - chr4.4:87333523-87333542 MS.gene068257:CDS 15.0%
!!! ACAGATTTTATATCATAAAA+TGG + chr4.4:87331618-87331637 None:intergenic 15.0%
!!! TGTTAAAAGAAACATTTTTT+AGG - chr4.4:87331153-87331172 MS.gene068257:intron 15.0%
!!! TTTTTTGAATTTATTTTGCA+GGG - chr4.4:87331304-87331323 MS.gene068257:intron 15.0%
!!! TTTTTTTGAATTTATTTTGC+AGG - chr4.4:87331303-87331322 MS.gene068257:intron 15.0%
!! AAATTATATGTCGCAAATAA+GGG - chr4.4:87333199-87333218 MS.gene068257:intron 20.0%
!! AGTTGAAAAAACAATATGTT+TGG - chr4.4:87332272-87332291 MS.gene068257:intron 20.0%
!! ATAACTTTGTATGTAATCTA+AGG - chr4.4:87331657-87331676 MS.gene068257:CDS 20.0%
!! ATAGAAGTAATATACAAAAC+TGG + chr4.4:87332230-87332249 None:intergenic 20.0%
!! ATCTTTGATATTCTCATTAA+TGG - chr4.4:87333583-87333602 MS.gene068257:intron 20.0%
!! ATTAACCAAAAAAAAAAGGT+TGG + chr4.4:87333822-87333841 None:intergenic 20.0%
!! CTCATTTAAAGAATGTTTAT+CGG - chr4.4:87333673-87333692 MS.gene068257:intron 20.0%
!! GAAAATGTCATATCTATATT+TGG - chr4.4:87333960-87333979 MS.gene068257:intron 20.0%
!! TTAATGAGAATATCAAAGAT+TGG + chr4.4:87333583-87333602 None:intergenic 20.0%
!!! AAGAACATACTTTCATTTTT+TGG - chr4.4:87331579-87331598 MS.gene068257:intron 20.0%
!!! ATGTATTTGATTTGATTTTC+AGG - chr4.4:87333035-87333054 MS.gene068257:intron 20.0%
!!! ATTTGATGGATTTTTTATTG+AGG - chr4.4:87331081-87331100 MS.gene068257:intron 20.0%
!!! ATTTTTTAGGTGATGAAAAA+TGG - chr4.4:87331166-87331185 MS.gene068257:intron 20.0%
!!! CATTGATTGAAAAAAGAAAA+GGG - chr4.4:87331009-87331028 MS.gene068257:intron 20.0%
!!! GAAAACTAAAATCTAAGATT+TGG + chr4.4:87331361-87331380 None:intergenic 20.0%
!!! GATATGTTTTTATTTGTACT+TGG - chr4.4:87332348-87332367 MS.gene068257:intron 20.0%
!!! TCATTGATTGAAAAAAGAAA+AGG - chr4.4:87331008-87331027 MS.gene068257:intron 20.0%
!!! TTTGATGGATTTTTTATTGA+GGG - chr4.4:87331082-87331101 MS.gene068257:intron 20.0%
!!! TTTTGTATATTACTTCTATC+TGG - chr4.4:87332231-87332250 MS.gene068257:intron 20.0%
! AAATGAGAGATTTACTTCAT+AGG - chr4.4:87333469-87333488 MS.gene068257:intron 25.0%
! AATCTTGAATTATTCTAGGT+TGG - chr4.4:87333886-87333905 MS.gene068257:intron 25.0%
! AATGAGAGATTTACTTCATA+GGG - chr4.4:87333470-87333489 MS.gene068257:intron 25.0%
! AATGAGTAAATATGAACCTT+TGG + chr4.4:87333659-87333678 None:intergenic 25.0%
! ATATGAAGAAATCTAAGAGT+TGG + chr4.4:87331227-87331246 None:intergenic 25.0%
! ATTATGAACTTTGTTAACCT+CGG - chr4.4:87332411-87332430 MS.gene068257:intron 25.0%
! CAAATTATATGTCGCAAATA+AGG - chr4.4:87333198-87333217 MS.gene068257:intron 25.0%
! CACTAAATTTGAGAATATTC+TGG + chr4.4:87332702-87332721 None:intergenic 25.0%
! CTTATTTGCGACATATAATT+TGG + chr4.4:87333200-87333219 None:intergenic 25.0%
! CTTGAATCTTGAATTATTCT+AGG - chr4.4:87333882-87333901 MS.gene068257:intron 25.0%
! TGACATTTACATCATAAACA+AGG + chr4.4:87332884-87332903 None:intergenic 25.0%
! TGATATTCTCATTAATGGTT+GGG - chr4.4:87333588-87333607 MS.gene068257:intron 25.0%
! TGATTTCACAACAAATAACT+AGG + chr4.4:87332553-87332572 None:intergenic 25.0%
! TTATGCATGTCATTATTCAT+TGG - chr4.4:87333432-87333451 MS.gene068257:intron 25.0%
! TTGATATTCTCATTAATGGT+TGG - chr4.4:87333587-87333606 MS.gene068257:intron 25.0%
! TTGTTAAAAGTCATTATCCT+CGG - chr4.4:87330889-87330908 MS.gene068257:CDS 25.0%
! TTTCAATTTCTTACTTCCAA+AGG - chr4.4:87333640-87333659 MS.gene068257:intron 25.0%
!! AAATTCGAAACATTGTTTTC+CGG - chr4.4:87331816-87331835 MS.gene068257:intron 25.0%
!! AACATTAAATCCTTCTTTTG+CGG + chr4.4:87333327-87333346 None:intergenic 25.0%
!! CAACAGTATGTTATTTACAT+CGG + chr4.4:87332764-87332783 None:intergenic 25.0%
!! TAATGACTTTTAACAACATG+CGG + chr4.4:87330885-87330904 None:intergenic 25.0%
!! TGATTTTCTCATCAATTGTT+TGG + chr4.4:87333509-87333528 None:intergenic 25.0%
!!! TGATACCAACCTTTTTTTTT+TGG - chr4.4:87333814-87333833 MS.gene068257:intron 25.0%
!!! TTGATGGATTTTTTATTGAG+GGG - chr4.4:87331083-87331102 MS.gene068257:intron 25.0%
AAAACTCACATTTCTTCTTC+AGG + chr4.4:87333395-87333414 None:intergenic 30.0%
AACTCTGTACTTTGAGTATT+TGG - chr4.4:87331916-87331935 MS.gene068257:CDS 30.0%
AAGTACAGAGTTTATGAGAT+AGG + chr4.4:87331908-87331927 None:intergenic 30.0%
AGTACAGAGTTTATGAGATA+GGG + chr4.4:87331907-87331926 None:intergenic 30.0%
ATATGCAATTAGTATGCCAT+TGG + chr4.4:87333157-87333176 None:intergenic 30.0%
CACTAGAAATTTGTATCGAA+TGG + chr4.4:87333747-87333766 None:intergenic 30.0%
GAAGTTTAGTAATCAGTACA+AGG - chr4.4:87331480-87331499 MS.gene068257:intron 30.0%
GTCAATTAGCAAATATAGCT+TGG + chr4.4:87331280-87331299 None:intergenic 30.0%
TATTTGTTCCGTTTGCATTT+AGG - chr4.4:87331446-87331465 MS.gene068257:intron 30.0%
TGACATGCATAAAGAAAACA+CGG + chr4.4:87333424-87333443 None:intergenic 30.0%
TGTTTCCCAATACAACAAAT+GGG + chr4.4:87333086-87333105 None:intergenic 30.0%
TTCACATACCTAAATGCAAA+CGG + chr4.4:87331457-87331476 None:intergenic 30.0%
TTGCATTTAGGTATGTGAAT+CGG - chr4.4:87331458-87331477 MS.gene068257:intron 30.0%
TTGTTTCCCAATACAACAAA+TGG + chr4.4:87333087-87333106 None:intergenic 30.0%
! ATTTGATAACCATGGTCAAA+AGG - chr4.4:87334061-87334080 MS.gene068257:intron 30.0%
! GTATTACTATCTTCTTTTGC+AGG - chr4.4:87333238-87333257 MS.gene068257:intron 30.0%
! TTGGTTAATTTTGTTCAGCA+AGG - chr4.4:87333833-87333852 MS.gene068257:intron 30.0%
!! AAACTTCCCATTTGTTGTAT+TGG - chr4.4:87333077-87333096 MS.gene068257:intron 30.0%
!! AACTTCCCATTTGTTGTATT+GGG - chr4.4:87333078-87333097 MS.gene068257:intron 30.0%
!! ATGAAATCTCATTTTGAGTC+TGG - chr4.4:87333783-87333802 MS.gene068257:intron 30.0%
!! CAATTGACTTGTTTGAAGTT+GGG + chr4.4:87331875-87331894 None:intergenic 30.0%
!! TCAATTGACTTGTTTGAAGT+TGG + chr4.4:87331876-87331895 None:intergenic 30.0%
!! TTGAATTATTCTAGGTTGGT+AGG - chr4.4:87333890-87333909 MS.gene068257:intron 30.0%
!! TTTTTCAACTTCAGAAACTC+AGG + chr4.4:87332262-87332281 None:intergenic 30.0%
!!! TTGAATTTATTTTGCAGGGT+TGG - chr4.4:87331308-87331327 MS.gene068257:intron 30.0%
AATTAGTATGCCATTGGAAG+AGG + chr4.4:87333151-87333170 None:intergenic 35.0%
AGGAAGTTCAATTTGAAGAC+AGG - chr4.4:87331530-87331549 MS.gene068257:intron 35.0%
ATTCTTCTCTCCAATGGTTA+AGG + chr4.4:87332923-87332942 None:intergenic 35.0%
CTAATTGTACACCCAGATTT+GGG - chr4.4:87332791-87332810 MS.gene068257:intron 35.0%
GAAAGATCAGTGTGTATGTT+TGG - chr4.4:87332660-87332679 MS.gene068257:intron 35.0%
GAGAGAAGAATTTCTCATTC+AGG - chr4.4:87332932-87332951 MS.gene068257:intron 35.0%
GGAAACAAAATAGACGTTGA+TGG - chr4.4:87333099-87333118 MS.gene068257:intron 35.0%
GGCATATGCATAACAAATCA+TGG - chr4.4:87333544-87333563 MS.gene068257:CDS 35.0%
GTAACCAGAAACTCAAAGTA+CGG + chr4.4:87332058-87332077 None:intergenic 35.0%
GTAATATACAAAACTGGACC+TGG + chr4.4:87332224-87332243 None:intergenic 35.0%
GTACAGAGTTTATGAGATAG+GGG + chr4.4:87331906-87331925 None:intergenic 35.0%
GTCTTCAAATTGAACTTCCT+TGG + chr4.4:87331530-87331549 None:intergenic 35.0%
TAACCAGAAACTCAAAGTAC+GGG + chr4.4:87332057-87332076 None:intergenic 35.0%
TCAAAGTTAGCTCAAGTGAA+AGG - chr4.4:87332492-87332511 MS.gene068257:intron 35.0%
TCGAGATTCAAACTGTGAAT+TGG + chr4.4:87334042-87334061 None:intergenic 35.0%
TCTAATTGTACACCCAGATT+TGG - chr4.4:87332790-87332809 MS.gene068257:intron 35.0%
TGAGAAATTCTTCTCTCCAA+TGG + chr4.4:87332929-87332948 None:intergenic 35.0%
! AACAAAATAGACGTTGATGG+TGG - chr4.4:87333102-87333121 MS.gene068257:intron 35.0%
! ACAACAAATGGGAAGTTTTC+AGG + chr4.4:87333075-87333094 None:intergenic 35.0%
! ACATTGTTTTCCGGTCTTTT+CGG - chr4.4:87331825-87331844 MS.gene068257:intron 35.0%
! ATGAAACTCGAGTTGTAAAC+AGG + chr4.4:87331712-87331731 None:intergenic 35.0%
! ATTAAATCCTTCTTTTGCGG+AGG + chr4.4:87333324-87333343 None:intergenic 35.0%
! CAACAAATGGGAAGTTTTCA+GGG + chr4.4:87333074-87333093 None:intergenic 35.0%
! GAATCTCGATTTGATAACCA+TGG - chr4.4:87334053-87334072 MS.gene068257:intron 35.0%
! TGGTGTTTGTTGATTATACC+AGG - chr4.4:87334092-87334111 MS.gene068257:intron 35.0%
!! ACAAAATAGACGTTGATGGT+GGG - chr4.4:87333103-87333122 MS.gene068257:intron 35.0%
!! ATGTCACTTTAATTTACCCG+TGG + chr4.4:87331737-87331756 None:intergenic 35.0%
!!! CAGATTGTTTTAAACGGAAG+AGG + chr4.4:87332193-87332212 None:intergenic 35.0%
!!! GTGCATTTTTTGCTATACAC+TGG + chr4.4:87333361-87333380 None:intergenic 35.0%
AACCAGAAACTCAAAGTACG+GGG + chr4.4:87332056-87332075 None:intergenic 40.0%
AACTCGAGTTTCATTTCCAC+GGG - chr4.4:87331718-87331737 MS.gene068257:CDS 40.0%
AATCAGTACAAGGCTACCAT+TGG - chr4.4:87331490-87331509 MS.gene068257:intron 40.0%
ATGATAGTTGACGATGGTTG+TGG - chr4.4:87332616-87332635 MS.gene068257:intron 40.0%
CAACTCGAGTTTCATTTCCA+CGG - chr4.4:87331717-87331736 MS.gene068257:CDS 40.0%
CTTTCGAGAACCTTAACCAT+TGG - chr4.4:87332910-87332929 MS.gene068257:intron 40.0%
GAACATTCCTAGAGTTGTGA+TGG + chr4.4:87331974-87331993 None:intergenic 40.0%
GAATGCATTTCCGAAAAGAC+CGG + chr4.4:87331838-87331857 None:intergenic 40.0%
GAGTTGTAAACAGGCTCTTA+TGG + chr4.4:87331703-87331722 None:intergenic 40.0%
GATTCAATCCTCAACGGAAA+TGG - chr4.4:87332160-87332179 MS.gene068257:CDS 40.0%
GGAACAAAACCATCCTTTCA+CGG + chr4.4:87332019-87332038 None:intergenic 40.0%
GTAAGTTACTCCTCTTCCAA+TGG - chr4.4:87333138-87333157 MS.gene068257:intron 40.0%
TCATTATCCTCGGTGATAGT+GGG - chr4.4:87330899-87330918 MS.gene068257:CDS 40.0%
TTCCTAGAGTTGTGATGGTA+TGG + chr4.4:87331969-87331988 None:intergenic 40.0%
TTGTGCATGATAGTTGACGA+TGG - chr4.4:87332610-87332629 MS.gene068257:intron 40.0%
! AGGATGGATTCAATCCTCAA+CGG - chr4.4:87332154-87332173 MS.gene068257:CDS 40.0%
! CCGTTTAAAACAATCTGCTG+CGG - chr4.4:87332196-87332215 MS.gene068257:CDS 40.0%
! GGTTTCTGAGAAGAAAGCAA+AGG - chr4.4:87333259-87333278 MS.gene068257:intron 40.0%
!!! CCGCAGCAGATTGTTTTAAA+CGG + chr4.4:87332199-87332218 None:intergenic 40.0%
AAGGCATGGTGTGCTTCAAA+GGG - chr4.4:87333278-87333297 MS.gene068257:intron 45.0%
ACCAGCAGTATCCCAAATCT+GGG + chr4.4:87332805-87332824 None:intergenic 45.0%
AGACAGGTTGTTCACATTGC+AGG - chr4.4:87331546-87331565 MS.gene068257:intron 45.0%
AGCCATACCATCACAACTCT+AGG - chr4.4:87331964-87331983 MS.gene068257:CDS 45.0%
ATGGCTGACTAAATGCGAGT+AGG + chr4.4:87331950-87331969 None:intergenic 45.0%
ATGGTCAAAAGGCTCAAGTG+TGG - chr4.4:87334072-87334091 MS.gene068257:intron 45.0%
CTGAGAAGAAAGCAAAGGCA+TGG - chr4.4:87333264-87333283 MS.gene068257:intron 45.0%
GATCAGAACCATTTCCGTTG+AGG + chr4.4:87332171-87332190 None:intergenic 45.0%
GGATTGAATCCATCCTTTCG+CGG + chr4.4:87332150-87332169 None:intergenic 45.0%
GGTGAGAAAATCAGCACCAA+TGG + chr4.4:87331509-87331528 None:intergenic 45.0%
GTCATTATCCTCGGTGATAG+TGG - chr4.4:87330898-87330917 MS.gene068257:CDS 45.0%
TTTGCGGAGGTCTCAAAGTA+TGG + chr4.4:87333311-87333330 None:intergenic 45.0%
! AAAGGCATGGTGTGCTTCAA+AGG - chr4.4:87333277-87333296 MS.gene068257:intron 45.0%
! AACCCCGTACTTTGAGTTTC+TGG - chr4.4:87332051-87332070 MS.gene068257:intron 45.0%
! ACACTTGAGCCTTTTGACCA+TGG + chr4.4:87334073-87334092 None:intergenic 45.0%
! ATTGCCGACATCGATTGTGT+CGG + chr4.4:87334125-87334144 None:intergenic 45.0%
! TTTGAGACCTCCGCAAAAGA+AGG - chr4.4:87333314-87333333 MS.gene068257:intron 45.0%
!!! TGGTGCTGATTTTCTCACCA+AGG - chr4.4:87331510-87331529 MS.gene068257:intron 45.0%
AAACAATCTGCTGCGGATCC+AGG - chr4.4:87332203-87332222 MS.gene068257:CDS 50.0%
ACAATCGATGTCGGCAATGG+TGG - chr4.4:87334127-87334146 MS.gene068257:intron 50.0%
CAAGAGCGGTTTCAGAGTCT+TGG - chr4.4:87332825-87332844 MS.gene068257:intron 50.0%
GACACAATCGATGTCGGCAA+TGG - chr4.4:87334124-87334143 MS.gene068257:intron 50.0%
GACCAGCAGTATCCCAAATC+TGG + chr4.4:87332806-87332825 None:intergenic 50.0%
GCTGACTAAATGCGAGTAGG+AGG + chr4.4:87331947-87331966 None:intergenic 50.0%
TACGGGGTTTACGCGATTTC+AGG + chr4.4:87332040-87332059 None:intergenic 50.0%
TTGAATCCATCCTTTCGCGG+CGG + chr4.4:87332147-87332166 None:intergenic 50.0%
! ACCCAGATTTGGGATACTGC+TGG - chr4.4:87332801-87332820 MS.gene068257:intron 50.0%
! GTCTTGGTGTGGCTTTCTAC+CGG - chr4.4:87332841-87332860 MS.gene068257:intron 50.0%
! GTTAACCTCGGCTGCTTTGA+CGG - chr4.4:87332423-87332442 MS.gene068257:intron 50.0%
! TCTTGGTGTGGCTTTCTACC+GGG - chr4.4:87332842-87332861 MS.gene068257:intron 50.0%
! TTAACCTCGGCTGCTTTGAC+GGG - chr4.4:87332424-87332443 MS.gene068257:intron 50.0%
! TTCCTAGAGTTGTGATGGCG+TGG + chr4.4:87332100-87332119 None:intergenic 50.0%
! TTGCCGACATCGATTGTGTC+GGG + chr4.4:87334124-87334143 None:intergenic 50.0%
!! GGGAAGTTTTCAGGGTCAGA+TGG + chr4.4:87333066-87333085 None:intergenic 50.0%
AGCCACGCCATCACAACTCT+AGG - chr4.4:87332095-87332114 MS.gene068257:intron 55.0%
CAGCATACCCACTATCACCG+AGG + chr4.4:87330909-87330928 None:intergenic 55.0%
CTGCCCGACACAATCGATGT+CGG - chr4.4:87334118-87334137 MS.gene068257:intron 55.0%
GATTGTGTCGGGCAGATACC+TGG + chr4.4:87334113-87334132 None:intergenic 55.0%
GCGGTTTCAGAGTCTTGGTG+TGG - chr4.4:87332830-87332849 MS.gene068257:intron 55.0%
GGACGACAACAGAGATCCAC+AGG - chr4.4:87334148-87334167 MS.gene068257:CDS 55.0%
GGGATACTGCTGGTCAAGAG+CGG - chr4.4:87332811-87332830 MS.gene068257:intron 55.0%
TACGTGTCGCCGTGAAAGGA+TGG - chr4.4:87332007-87332026 MS.gene068257:intron 55.0%
TCCTTTCACGGCGACACGTA+AGG + chr4.4:87332007-87332026 None:intergenic 55.0%
! CTTGGTGTGGCTTTCTACCG+GGG - chr4.4:87332843-87332862 MS.gene068257:intron 55.0%
! TTTTCCCGTCAAAGCAGCCG+AGG + chr4.4:87332431-87332450 None:intergenic 55.0%
GCCTTACGTGTCGCCGTGAA+AGG - chr4.4:87332003-87332022 MS.gene068257:intron 60.0%
GGCGACACGTAAGGCAGTGA+AGG + chr4.4:87331998-87332017 None:intergenic 60.0%
ATCCTTTCGCGGCGGCGCAT+AGG + chr4.4:87332139-87332158 None:intergenic 65.0%
GGACGCAGCAATCAGCACCC+CGG + chr4.4:87332863-87332882 None:intergenic 65.0%
TATGCGCCGCCGCGAAAGGA+TGG - chr4.4:87332138-87332157 MS.gene068257:CDS 65.0%
TCCTTTCGCGGCGGCGCATA+GGG + chr4.4:87332138-87332157 None:intergenic 65.0%
GGCGGCGCATAGGGCAGTGA+AGG + chr4.4:87332129-87332148 None:intergenic 70.0%
GCCCTATGCGCCGCCGCGAA+AGG - chr4.4:87332134-87332153 MS.gene068257:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 87330868 87334183 87330868 ID=MS.gene068257
chr4.4 mRNA 87330868 87334183 87330868 ID=MS.gene068257.t1;Parent=MS.gene068257
chr4.4 exon 87334131 87334183 87334131 ID=MS.gene068257.t1.exon1;Parent=MS.gene068257.t1
chr4.4 CDS 87334131 87334183 87334131 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87333700 87333726 87333700 ID=MS.gene068257.t1.exon2;Parent=MS.gene068257.t1
chr4.4 CDS 87333700 87333726 87333700 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87333484 87333583 87333484 ID=MS.gene068257.t1.exon3;Parent=MS.gene068257.t1
chr4.4 CDS 87333484 87333583 87333484 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87332098 87332244 87332098 ID=MS.gene068257.t1.exon4;Parent=MS.gene068257.t1
chr4.4 CDS 87332098 87332244 87332098 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87331914 87331994 87331914 ID=MS.gene068257.t1.exon5;Parent=MS.gene068257.t1
chr4.4 CDS 87331914 87331994 87331914 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87331646 87331791 87331646 ID=MS.gene068257.t1.exon6;Parent=MS.gene068257.t1
chr4.4 CDS 87331646 87331791 87331646 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
chr4.4 exon 87330868 87330937 87330868 ID=MS.gene068257.t1.exon7;Parent=MS.gene068257.t1
chr4.4 CDS 87330868 87330937 87330868 ID=cds.MS.gene068257.t1;Parent=MS.gene068257.t1
Gene Sequence

>MS.gene068257

ATGGCTTCTCGACGCCGCATGTTGTTAAAAGTCATTATCCTCGGTGATAGTGGGTATGCTGCTTCTTTTTCAATTTTTTTTCGATTTTTCTTCTTGCAGTTTTTAGTTTTGAATTTAATCATCAAATCAGTTATATCACTTCATTGATTGAAAAAAGAAAAGGGTTGTTGCATTTAATCTTAAAGTTTACTTTTTTAATTCAAATTTAACAAAATTTGATGGATTTTTTATTGAGGGGAAATTATTAATGATCATGTTTAGATTCTTGAAAATATGAAACTTTATTGTTAAAAGAAACATTTTTTAGGTGATGAAAAATGGAAAAATAGTTATAGATTCAATCGATTAACAATTTTCCAACTCTTAGATTTCTTCATATCAATTGTAGATGCAATTTTGTTGTTAAGAACCAAGCTATATTTGCTAATTGACATTTTTTTTTGAATTTATTTTGCAGGGTTGGTAAAACATCATTGATGAATCAGTATGTCCAAATCTTAGATTTTAGTTTTCTTTAATATTTTGTTGAAATGTTGAGTTTTGACATTTTTTTTATTGATTTGTATTGTCATAATTGTTATTTGTTCCGTTTGCATTTAGGTATGTGAATCGGAAGTTTAGTAATCAGTACAAGGCTACCATTGGTGCTGATTTTCTCACCAAGGAAGTTCAATTTGAAGACAGGTTGTTCACATTGCAGGTGAGAGACACAAGAACATACTTTCATTTTTTGGATAAAAATTTGTGCCATTTTATGATATAAAATCTGTTATTTTGATCAATTTTATCATAACTTTGTATGTAATCTAAGGTGAATTTTAGCATAGAGAAACCATAAGAGCCTGTTTACAACTCGAGTTTCATTTCCACGGGTAAATTAAAGTGACATTTTGAAAGTTTCTTAAAATGAATCTAAAAATAGATTGAATATTTTTTAAAATTTTAAAGAAATTCGAAACATTGTTTTCCGGTCTTTTCGGAAATGCATTCTCTCTAGTTAGCATCCCAACTTCAAACAAGTCAATTGATCTCAAACCCCTATCTCATAAACTCTGTACTTTGAGTATTTGGTTACTCCTCCTACTCGCATTTAGTCAGCCATACCATCACAACTCTAGGAATGTTCACCTTCACTGCCTTACGTGTCGCCGTGAAAGGATGGTTTTGTTCCTGAAATCGCGTAAACCCCGTACTTTGAGTTTCTGGTTACTCTAGTCGCATTTAGTCAGCCACGCCATCACAACTCTAGGAATGTTCTCCTTCACTGCCCTATGCGCCGCCGCGAAAGGATGGATTCAATCCTCAACGGAAATGGTTCTGATCCTCTTCCGTTTAAAACAATCTGCTGCGGATCCAGGTCCAGTTTTGTATATTACTTCTATCTGGAAGCTCCTGAGTTTCTGAAGTTGAAAAAACAATATGTTTGGTCTTTTTTGAAGATCTAACAAACTGATTAGTTTGTTTGCTGATATGTTTTGCTGATATGTTTTTATTTGTACTTGGCTGTTACAAAATTGCTTGTTCTTCAGATTTCATGAAGTGAATTATGAACTTTGTTAACCTCGGCTGCTTTGACGGGAAAATTCTCAAATTTCGATTTGTTGTCAATACTAACAGATTATCGTCAAAGTTAGCTCAAGTGAAAGGTTATAAAATTAAAATTTGGAAAGGAAGTAAAGTATCCTAGTTATTTGTTGTGAAATCAATGATTTTATGCTGTATGAACTATAAAATGTTTTTCATTGTGCATGATAGTTGACGATGGTTGTGGTTAGTTCACTTTCTACTTGATGAAAGATCAGTGTGTATGTTTGGCTGTGTTTATTTTTTGCCAGAATATTCTCAAATTTAGTGCATGTTATAATTAATAATTGTCTAAGATTTAGATGTTCTCCGATGTAAATAACATACTGTTGTTGAATTCTAATTGTACACCCAGATTTGGGATACTGCTGGTCAAGAGCGGTTTCAGAGTCTTGGTGTGGCTTTCTACCGGGGTGCTGATTGCTGCGTCCTTGTTTATGATGTAAATGTCATGAAATCTTTCGAGAACCTTAACCATTGGAGAGAAGAATTTCTCATTCAGGTAATTTTGTTTAATCATTAAAAACAGTCTTTGTTTTATCTCCATATCTCTTTTTATTTAAGAACATTGAGAAACTCTTGCATGTATTTGATTTGATTTTCAGGCTAGTCCATCTGACCCTGAAAACTTCCCATTTGTTGTATTGGGAAACAAAATAGACGTTGATGGTGGGAATAGCAGAGTTGTAAGTTACTCCTCTTCCAATGGCATACTAATTGCATATTGACTTGTATGAAAATTAAGCCAAATTATATGTCGCAAATAAGGGTTTGTTTGATTCATTAGTATTACTATCTTCTTTTGCAGGTTTCTGAGAAGAAAGCAAAGGCATGGTGTGCTTCAAAGGGAAACATACCATACTTTGAGACCTCCGCAAAAGAAGGATTTAATGTTGAAGCTGCTTTCCAGTGTATAGCAAAAAATGCACTCAAGAATGAACCTGAAGAAGAAATGTGAGTTTTCTTTAACCGTGTTTTCTTTATGCATGTCATTATTCATTGGATTTGACAACTCTAAAATGAGAGATTTACTTCATAGGGTAAATTAAGTAATCCAAACAATTGATGAGAAAATCAATGAATGATTTTAAGGGCATATGCATAACAAATCATGGATGTGTTCGAATACCAATCTTTGATATTCTCATTAATGGTTGGGATAGCAGAACTAAATCCTTATTCATTAATTTTCAATTTCTTACTTCCAAAGGTTCATATTTACTCATTTAAAGAATGTTTATCGGTATCTTACAATACATGCGATTCGTATCTCAATTCAATAACAACTAAATCCATTCGATACAAATTTCTAGTGTGTATCGCTTTTGAACATGAAATCTCATTTTGAGTCTGGATTCACTATGATACCAACCTTTTTTTTTTGGTTAATTTTGTTCAGCAAGGCATTTTTCTTGTCATTTGATTTATTACTTGAATCTTGAATTATTCTAGGTTGGTAGGTGATATATTGCTTATAATTTTCTATATAACTTGTCTTGTCTTTACTTGAAAATGTCATATCTATATTTGGTAATGTATCTTACAGTTCATAATAAGATTCAATTCACTGTTTCAAAAGTATGTCTTCCAATTCACAGTTTGAATCTCGATTTGATAACCATGGTCAAAAGGCTCAAGTGTGGTGTTTGTTGATTATACCAGGTATCTGCCCGACACAATCGATGTCGGCAATGGTGGACGACAACAGAGATCCACAGGCTGTGAATGTTGA
Protein sequence

>MS.gene068257.t1

MASRRRMLLKVIILGDSGVGKTSLMNQYVNRKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNVMKSFENLNHWREEFLIQASPSDPENFPFVVLGNKIDVDGGNSRVVSEKKAKAWCASKGNIPYFETSAKEGFNVEAAFQCIAKNALKNEPEEEMYLPDTIDVGNGGRQQRSTGCEC