AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34075

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34075.t1 MTR_1g094220 99.024 205 1 1 1 205 1 204 4.83e-150 414
MS.gene34075.t1 MTR_1g094220 98.421 190 2 1 1 190 1 189 6.24e-138 384
MS.gene34075.t1 MTR_1g094220 98.936 188 1 1 1 188 1 187 1.04e-136 381
MS.gene34075.t1 MTR_1g094220 98.936 188 1 1 1 188 1 187 7.62e-136 380
MS.gene34075.t1 MTR_3g064390 63.975 161 57 1 30 190 8 167 1.29e-73 221
MS.gene34075.t1 MTR_5g076590 62.353 170 59 2 30 199 8 172 1.83e-73 221
MS.gene34075.t1 MTR_2g102060 59.877 162 64 1 29 190 7 167 2.65e-69 210
MS.gene34075.t1 MTR_8g103550 56.024 166 72 1 25 190 2 166 8.72e-69 209
MS.gene34075.t1 MTR_3g064390 66.154 130 43 1 30 159 8 136 5.05e-59 182
MS.gene34075.t1 MTR_2g005510 48.171 164 84 1 25 188 8 170 1.47e-50 163
MS.gene34075.t1 MTR_8g089985 47.126 174 87 2 22 191 3 175 2.28e-50 163
MS.gene34075.t1 MTR_8g036220 50.000 154 76 1 34 187 18 170 6.51e-50 162
MS.gene34075.t1 MTR_2g075950 47.468 158 82 1 34 191 15 171 7.37e-49 159
MS.gene34075.t1 MTR_2g083860 46.203 158 84 1 34 191 15 171 1.18e-48 158
MS.gene34075.t1 MTR_3g087160 47.170 159 83 1 32 190 9 166 1.20e-48 158
MS.gene34075.t1 MTR_3g086980 48.101 158 81 1 34 191 18 174 2.05e-48 157
MS.gene34075.t1 MTR_8g090215 47.170 159 83 1 32 190 9 166 2.09e-48 157
MS.gene34075.t1 MTR_4g064897 48.101 158 81 1 34 191 14 170 2.53e-48 157
MS.gene34075.t1 MTR_5g013900 47.170 159 83 1 32 190 9 166 2.65e-48 157
MS.gene34075.t1 MTR_3g069510 48.101 158 81 1 34 191 14 170 6.20e-48 156
MS.gene34075.t1 MTR_6g021790 42.857 154 87 1 34 187 8 160 2.05e-47 155
MS.gene34075.t1 MTR_8g031660 42.857 154 87 1 34 187 8 160 1.42e-46 152
MS.gene34075.t1 MTR_2g014990 46.203 158 84 1 34 191 15 171 2.00e-46 152
MS.gene34075.t1 MTR_8g031870 44.156 154 85 1 34 187 8 160 5.46e-46 150
MS.gene34075.t1 MTR_2g027660 42.208 154 88 1 34 187 8 160 7.12e-46 151
MS.gene34075.t1 MTR_4g098950 45.283 159 86 1 32 190 9 166 7.32e-46 150
MS.gene34075.t1 MTR_4g079350 45.570 158 85 1 34 191 15 171 8.84e-46 151
MS.gene34075.t1 MTR_4g088090 44.937 158 86 1 34 191 15 171 1.11e-45 150
MS.gene34075.t1 MTR_0262s0050 45.570 158 85 1 34 191 17 173 1.50e-45 150
MS.gene34075.t1 MTR_7g405790 42.941 170 96 1 34 203 17 185 1.64e-45 150
MS.gene34075.t1 MTR_1g052475 44.937 158 86 1 34 191 17 173 2.14e-45 150
MS.gene34075.t1 MTR_7g051940 42.941 170 96 1 34 203 15 183 2.93e-45 149
MS.gene34075.t1 MTR_8g031680 42.857 154 87 1 34 187 8 160 3.29e-45 149
MS.gene34075.t1 MTR_2g090865 44.937 158 86 1 34 191 13 169 1.79e-44 147
MS.gene34075.t1 MTR_1g078200 43.275 171 91 2 34 204 16 180 3.52e-44 147
MS.gene34075.t1 MTR_1g068730 45.283 159 85 2 33 190 16 173 7.51e-44 146
MS.gene34075.t1 MTR_1g068740 45.283 159 85 2 33 190 16 173 1.06e-43 145
MS.gene34075.t1 MTR_1g115325 45.283 159 85 2 33 190 16 173 1.20e-43 145
MS.gene34075.t1 MTR_7g100020 45.283 159 85 2 33 190 16 173 1.69e-43 145
MS.gene34075.t1 MTR_8g027220 44.785 163 89 1 34 196 28 189 4.34e-43 144
MS.gene34075.t1 MTR_8g031830 41.250 160 92 2 34 192 8 166 6.58e-43 143
MS.gene34075.t1 MTR_6g079550 44.937 158 86 1 34 191 14 170 2.45e-42 142
MS.gene34075.t1 MTR_8g006970 43.506 154 86 1 34 187 10 162 1.50e-41 139
MS.gene34075.t1 MTR_8g006970 43.506 154 86 1 34 187 24 176 3.26e-41 139
MS.gene34075.t1 MTR_4g063160 44.720 161 85 3 33 190 16 175 3.49e-41 139
MS.gene34075.t1 MTR_7g081700 42.857 154 87 1 34 187 10 162 3.59e-41 139
MS.gene34075.t1 MTR_2g005500 40.909 154 90 1 34 187 11 163 1.66e-38 132
MS.gene34075.t1 MTR_2g096550 41.420 169 96 3 33 199 16 183 2.56e-38 132
MS.gene34075.t1 MTR_8g093740 39.884 173 100 4 21 191 2 172 1.00e-37 130
MS.gene34075.t1 MTR_1g068730 43.077 130 73 1 33 162 16 144 4.32e-36 124
MS.gene34075.t1 MTR_1g068740 44.800 125 68 1 33 157 16 139 1.13e-35 124
MS.gene34075.t1 MTR_3g096350 41.250 160 90 4 34 191 14 171 2.43e-35 124
MS.gene34075.t1 MTR_8g107070 44.304 158 87 1 34 191 16 172 3.41e-35 125
MS.gene34075.t1 MTR_7g103070 38.037 163 98 3 30 190 14 175 1.19e-34 122
MS.gene34075.t1 MTR_1g029450 40.000 160 92 4 34 191 19 176 3.95e-34 121
MS.gene34075.t1 MTR_7g117910 40.426 141 83 1 34 174 22 161 6.97e-34 120
MS.gene34075.t1 MTR_1g062760 37.805 164 96 5 30 190 12 172 1.33e-32 117
MS.gene34075.t1 MTR_1g090717 39.752 161 91 5 34 190 17 175 6.53e-32 115
MS.gene34075.t1 MTR_4g099470 41.935 124 71 1 64 187 16 138 2.51e-31 112
MS.gene34075.t1 MTR_2g101890 33.516 182 113 4 29 203 7 187 5.30e-29 107
MS.gene34075.t1 MTR_4g069850 35.152 165 99 4 33 190 9 172 1.79e-28 106
MS.gene34075.t1 MTR_1g097420 37.037 162 94 4 33 187 9 169 2.59e-28 105
MS.gene34075.t1 MTR_4g012940 33.146 178 111 4 29 199 5 181 4.05e-28 105
MS.gene34075.t1 MTR_8g092020 34.940 166 100 5 29 187 9 173 5.04e-27 102
MS.gene34075.t1 MTR_8g093740 39.231 130 76 3 64 191 11 139 1.44e-26 100
MS.gene34075.t1 MTR_7g103070 41.441 111 63 2 82 190 9 119 4.25e-26 99.0
MS.gene34075.t1 MTR_3g078260 35.119 168 94 5 33 188 9 173 3.96e-25 97.8
MS.gene34075.t1 MTR_2g090875 34.320 169 94 7 33 188 7 171 2.50e-23 92.8
MS.gene34075.t1 MTR_3g096350 41.525 118 65 4 34 149 14 129 5.50e-23 90.5
MS.gene34075.t1 MTR_3g096350 41.525 118 65 4 34 149 14 129 6.97e-23 90.1
MS.gene34075.t1 MTR_4g088055 32.571 175 101 7 33 194 7 177 9.79e-23 91.3
MS.gene34075.t1 MTR_6g087980 32.164 171 101 6 33 191 7 174 1.29e-22 91.3
MS.gene34075.t1 MTR_4g073250 33.140 172 98 7 33 191 7 174 1.85e-22 90.5
MS.gene34075.t1 MTR_1g097420 35.526 152 90 4 43 187 46 196 2.05e-22 91.3
MS.gene34075.t1 MTR_8g075240 33.728 169 95 7 33 188 7 171 4.36e-22 89.4
MS.gene34075.t1 MTR_5g022600 32.544 169 97 7 33 188 7 171 7.47e-22 89.0
MS.gene34075.t1 MTR_6g039490 33.333 165 96 5 33 187 9 169 5.46e-21 87.0
MS.gene34075.t1 MTR_3g078260 37.931 116 68 3 33 147 9 121 2.10e-18 78.6
MS.gene34075.t1 MTR_3g107717 30.519 154 103 4 34 187 15 164 2.14e-18 80.1
MS.gene34075.t1 MTR_3g107707 30.519 154 103 4 34 187 15 164 2.52e-18 80.1
MS.gene34075.t1 MTR_3g107713 30.519 154 103 4 34 187 15 164 2.92e-18 79.7
MS.gene34075.t1 MTR_4g113620 28.571 154 97 5 34 187 15 155 9.39e-15 70.1
MS.gene34075.t1 MTR_8g031810 31.683 101 54 2 36 136 10 95 2.88e-14 66.2
MS.gene34075.t1 MTR_1452s0010 37.349 83 51 1 52 134 1 82 4.40e-14 65.9
MS.gene34075.t1 MTR_5g092060 26.404 178 120 5 21 191 93 266 6.67e-12 63.5
MS.gene34075.t1 MTR_1686s0010 35.000 80 51 1 52 131 1 79 1.23e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34075.t1 AT3G54840 86.911 191 24 1 1 190 1 191 1.37e-119 337
MS.gene34075.t1 AT3G54840 87.302 189 23 1 1 188 1 189 4.49e-119 336
MS.gene34075.t1 AT3G54840 92.949 156 11 0 35 190 2 157 8.80e-106 301
MS.gene34075.t1 AT4G19640 59.259 162 65 1 29 190 7 167 1.40e-68 208
MS.gene34075.t1 AT5G45130 59.259 162 65 1 29 190 7 167 1.49e-67 206
MS.gene34075.t1 AT5G45130 59.355 155 62 1 36 190 22 175 1.56e-63 196
MS.gene34075.t1 AT4G19640 59.542 131 52 1 29 159 7 136 2.37e-53 168
MS.gene34075.t1 AT4G19640 59.542 131 52 1 29 159 7 136 1.25e-52 167
MS.gene34075.t1 AT1G09630 50.000 158 78 1 34 191 14 170 8.27e-51 164
MS.gene34075.t1 AT1G07410 47.093 172 90 1 34 205 14 184 1.76e-49 160
MS.gene34075.t1 AT3G46830 48.101 158 81 1 34 191 14 170 2.46e-48 157
MS.gene34075.t1 AT3G46830 48.101 158 81 1 34 191 14 170 2.46e-48 157
MS.gene34075.t1 AT4G39990 45.570 158 85 1 34 191 19 175 3.83e-48 157
MS.gene34075.t1 AT5G59150 47.468 158 82 1 34 191 14 170 1.10e-47 155
MS.gene34075.t1 AT3G15060 47.468 158 82 1 34 191 15 171 1.61e-47 155
MS.gene34075.t1 AT1G16920 46.835 158 83 1 34 191 15 171 1.92e-47 155
MS.gene34075.t1 AT5G10260 45.912 159 85 1 32 190 9 166 2.30e-47 154
MS.gene34075.t1 AT5G65270 46.203 158 84 1 34 191 19 175 2.45e-47 155
MS.gene34075.t1 AT4G18430 45.570 158 85 1 34 191 15 171 5.90e-47 154
MS.gene34075.t1 AT2G22290 45.912 159 85 1 32 190 9 166 6.18e-47 154
MS.gene34075.t1 AT5G45750 44.937 158 86 1 34 191 15 171 8.66e-47 153
MS.gene34075.t1 AT3G07410 46.835 158 83 1 34 191 14 170 1.12e-46 153
MS.gene34075.t1 AT5G60860 46.203 158 84 1 34 191 15 171 2.03e-46 152
MS.gene34075.t1 AT2G43130 44.937 158 86 1 34 191 14 170 2.34e-46 152
MS.gene34075.t1 AT2G44610 45.912 159 85 1 32 190 9 166 3.38e-46 152
MS.gene34075.t1 AT4G39890 43.750 160 88 2 32 190 9 167 5.50e-46 151
MS.gene34075.t1 AT4G35860 41.558 154 89 1 34 187 8 160 6.76e-46 151
MS.gene34075.t1 AT3G12160 45.570 158 85 1 34 191 17 173 7.00e-46 151
MS.gene34075.t1 AT4G17160 42.308 156 89 1 32 187 6 160 7.61e-46 150
MS.gene34075.t1 AT2G33870 45.570 158 85 1 34 191 15 171 1.05e-45 150
MS.gene34075.t1 AT1G28550 45.570 158 85 1 34 191 15 171 1.62e-45 150
MS.gene34075.t1 AT4G18800 43.038 158 89 1 34 191 15 171 2.18e-45 150
MS.gene34075.t1 AT5G47960 46.104 154 82 1 34 187 17 169 2.20e-45 150
MS.gene34075.t1 AT4G17170 41.558 154 89 1 34 187 8 160 4.00e-45 149
MS.gene34075.t1 AT1G06400 44.304 158 87 1 34 191 15 171 6.67e-45 149
MS.gene34075.t1 AT5G47520 42.442 172 98 1 34 205 16 186 9.34e-45 148
MS.gene34075.t1 AT5G64990 45.223 157 85 1 34 190 9 164 2.07e-44 147
MS.gene34075.t1 AT1G05810 43.671 158 88 1 34 191 14 170 1.20e-43 145
MS.gene34075.t1 AT3G53610 45.912 159 84 2 33 190 16 173 1.29e-43 145
MS.gene34075.t1 AT3G53610 45.912 159 84 2 33 190 16 173 1.29e-43 145
MS.gene34075.t1 AT3G53610 45.912 159 84 2 33 190 16 173 1.29e-43 145
MS.gene34075.t1 AT3G46060 45.283 159 85 2 33 190 16 173 1.87e-43 145
MS.gene34075.t1 AT3G46060 45.283 159 85 2 33 190 16 173 1.87e-43 145
MS.gene34075.t1 AT3G46060 45.283 159 85 2 33 190 16 173 1.87e-43 145
MS.gene34075.t1 AT1G05810 43.671 158 88 1 34 191 60 216 2.22e-43 146
MS.gene34075.t1 AT5G59840 45.283 159 85 2 33 190 16 173 3.18e-43 144
MS.gene34075.t1 AT2G31680 41.040 173 98 2 19 191 2 170 4.20e-43 144
MS.gene34075.t1 AT1G02130 44.156 154 85 1 34 187 10 162 2.98e-42 141
MS.gene34075.t1 AT1G18200 45.570 158 85 1 34 191 15 171 3.12e-42 142
MS.gene34075.t1 AT4G17530 43.506 154 86 1 34 187 10 162 5.31e-42 140
MS.gene34075.t1 AT5G64990 43.293 164 85 2 34 190 9 171 1.19e-41 140
MS.gene34075.t1 AT5G47200 43.506 154 86 1 34 187 10 162 1.20e-41 140
MS.gene34075.t1 AT1G01200 44.578 166 90 2 34 198 30 194 1.32e-40 138
MS.gene34075.t1 AT3G09900 44.025 159 87 2 33 190 16 173 1.64e-39 135
MS.gene34075.t1 AT3G11730 40.606 165 97 1 34 198 10 173 2.77e-39 134
MS.gene34075.t1 AT1G73640 44.156 154 85 1 34 187 15 167 3.31e-39 134
MS.gene34075.t1 AT5G03520 44.025 159 87 2 33 190 16 173 6.23e-39 133
MS.gene34075.t1 AT1G43890 39.053 169 99 4 25 191 6 172 2.24e-35 124
MS.gene34075.t1 AT1G43890 39.053 169 99 4 25 191 6 172 2.24e-35 124
MS.gene34075.t1 AT1G43890 39.053 169 99 4 25 191 6 172 2.24e-35 124
MS.gene34075.t1 AT5G59150 48.649 111 57 0 81 191 9 119 2.16e-33 117
MS.gene34075.t1 AT4G35860 43.636 110 62 0 78 187 5 114 1.02e-32 115
MS.gene34075.t1 AT5G03530 36.310 168 103 4 25 190 6 171 6.71e-32 115
MS.gene34075.t1 AT5G03530 36.310 168 103 4 25 190 6 171 6.71e-32 115
MS.gene34075.t1 AT3G18820 36.364 165 97 4 33 190 9 172 5.28e-30 110
MS.gene34075.t1 AT1G22740 36.686 169 99 4 33 194 9 176 1.13e-29 109
MS.gene34075.t1 AT2G21880 35.088 171 103 4 33 196 10 179 1.64e-29 109
MS.gene34075.t1 AT3G09910 36.905 168 102 4 25 190 6 171 1.85e-28 106
MS.gene34075.t1 AT3G09910 36.905 168 102 4 25 190 6 171 1.85e-28 106
MS.gene34075.t1 AT3G09910 36.905 168 102 4 25 190 6 171 1.85e-28 106
MS.gene34075.t1 AT1G52280 34.270 178 109 4 29 199 5 181 1.88e-28 106
MS.gene34075.t1 AT3G09910 36.905 168 102 4 25 190 6 171 2.34e-28 105
MS.gene34075.t1 AT3G09910 36.905 168 102 4 25 190 6 171 2.34e-28 105
MS.gene34075.t1 AT1G49300 35.185 162 97 4 33 187 9 169 8.65e-28 104
MS.gene34075.t1 AT1G49300 35.185 162 97 4 33 187 9 169 8.65e-28 104
MS.gene34075.t1 AT4G09720 34.911 169 102 4 33 194 9 176 1.62e-27 103
MS.gene34075.t1 AT3G16100 33.708 178 110 4 29 199 5 181 2.31e-27 103
MS.gene34075.t1 AT5G03520 42.276 123 69 2 69 190 42 163 8.11e-27 102
MS.gene34075.t1 AT4G09720 33.714 175 101 5 33 194 9 181 9.89e-25 96.7
MS.gene34075.t1 AT5G39620 31.395 172 110 4 32 196 5 175 3.14e-24 95.1
MS.gene34075.t1 AT4G09720 32.778 180 102 5 33 194 9 187 4.57e-24 95.1
MS.gene34075.t1 AT3G48040 35.503 169 92 7 33 188 9 173 4.63e-24 94.7
MS.gene34075.t1 AT5G62880 34.302 172 96 7 33 191 9 176 6.54e-24 94.7
MS.gene34075.t1 AT2G44690 34.884 172 95 7 33 191 19 186 1.98e-23 93.2
MS.gene34075.t1 AT3G51300 34.320 169 94 7 33 188 7 171 2.65e-23 92.8
MS.gene34075.t1 AT1G75840 34.320 169 94 7 33 188 7 171 3.52e-23 92.4
MS.gene34075.t1 AT1G20090 34.320 169 94 7 33 188 6 170 3.91e-23 92.0
MS.gene34075.t1 AT4G35020 34.911 169 93 7 33 188 7 171 5.75e-23 91.7
MS.gene34075.t1 AT4G35020 34.911 169 93 7 33 188 7 171 5.75e-23 91.7
MS.gene34075.t1 AT4G35020 34.911 169 93 7 33 188 7 171 5.75e-23 91.7
MS.gene34075.t1 AT5G45970 33.714 175 99 7 33 194 7 177 1.70e-22 90.5
MS.gene34075.t1 AT2G17800 33.728 169 95 7 33 188 7 171 3.90e-22 89.7
MS.gene34075.t1 AT2G17800 33.728 169 95 7 33 188 7 171 3.90e-22 89.7
MS.gene34075.t1 AT2G21880 33.333 153 94 4 51 196 3 154 6.12e-22 89.0
MS.gene34075.t1 AT2G21880 33.333 153 94 4 51 196 3 154 6.12e-22 89.0
MS.gene34075.t1 AT3G48040 36.250 160 85 7 33 179 9 164 7.94e-22 88.2
MS.gene34075.t1 AT5G62880 35.625 160 86 7 33 179 9 164 1.21e-21 88.2
MS.gene34075.t1 AT4G35950 33.136 169 96 7 33 188 7 171 1.49e-21 88.2
MS.gene34075.t1 AT4G28950 31.361 169 103 6 33 191 7 172 2.56e-21 87.8
MS.gene34075.t1 AT5G55080 29.870 154 104 4 34 187 15 164 2.89e-20 85.1
MS.gene34075.t1 AT2G21880 29.240 171 105 4 33 196 10 171 7.41e-20 84.0
MS.gene34075.t1 AT5G46025 43.333 90 50 1 103 191 14 103 5.05e-19 79.3
MS.gene34075.t1 AT4G09720 34.286 140 84 4 62 194 4 142 9.04e-19 80.1
MS.gene34075.t1 AT4G09720 34.286 140 84 4 62 194 4 142 9.04e-19 80.1
MS.gene34075.t1 AT4G09720 34.286 140 84 4 62 194 4 142 9.04e-19 80.1
MS.gene34075.t1 AT4G35020 40.171 117 64 5 33 147 7 119 1.60e-18 79.0
MS.gene34075.t1 AT4G35020 40.171 117 64 5 33 147 7 119 1.60e-18 79.0
MS.gene34075.t1 AT5G20020 30.519 154 103 4 34 187 15 164 2.26e-18 80.1
MS.gene34075.t1 AT5G20010 30.519 154 103 4 34 187 15 164 2.81e-18 80.1
MS.gene34075.t1 AT5G55190 30.519 154 103 4 34 187 15 164 3.22e-18 79.7
MS.gene34075.t1 AT3G51290 34.146 123 68 4 78 188 651 772 7.26e-15 72.8

Find 51 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGCTTTGCTGAAAGATTC+TGG 0.171304 1.2:-74253522 None:intergenic
CTTGTCCTCTTAGGTGATTC+TGG 0.190714 1.2:+74252976 MS.gene34075:CDS
ACCAATGCCATCACTATATC+AGG 0.244974 1.2:-74254487 None:intergenic
TCCTCCACCATTCTCGGGAC+TGG 0.283720 1.2:-74251974 None:intergenic
CAACAGTCAAGTTTGAAATA+TGG 0.289384 1.2:+74253262 MS.gene34075:CDS
TGAACACAGCTTGTCCTCTT+AGG 0.360886 1.2:+74252967 MS.gene34075:intron
TATATTATCCGCTGTCTTTG+CGG 0.366407 1.2:-74254695 None:intergenic
TCAGGCAGCAAGAACATCAT+AGG 0.371455 1.2:-74255252 None:intergenic
CCTCCACCATTCTCGGGACT+GGG 0.380240 1.2:-74251973 None:intergenic
GTGCTTTGCTGAAAGATTCT+GGG 0.381291 1.2:-74253521 None:intergenic
GTCTTGTCCTCCACCATTCT+CGG 0.385898 1.2:-74251980 None:intergenic
GGTTGCGGCTCCTCCTTTCC+AGG 0.386599 1.2:+74251720 MS.gene34075:CDS
AACAGTCAAGTTTGAAATAT+GGG 0.387955 1.2:+74253263 MS.gene34075:CDS
CTTTCAGCAAAGCACAGTAC+TGG 0.403439 1.2:+74253529 MS.gene34075:CDS
CTGTTTCAGGTATGCTGCAT+TGG 0.410169 1.2:+74253443 MS.gene34075:intron
CATATTCTTACCTTGGATGT+TGG 0.419450 1.2:-74253048 None:intergenic
CTCTTAGGTGATTCTGGTGT+TGG 0.430297 1.2:+74252982 MS.gene34075:CDS
CAAAGCACAGTACTGGGTTA+AGG 0.458693 1.2:+74253536 MS.gene34075:CDS
TCTTGTCCTCCACCATTCTC+GGG 0.488194 1.2:-74251979 None:intergenic
TAATATAAACGAACTGTTTG+AGG 0.496118 1.2:+74254711 MS.gene34075:CDS
ATTGTTCTGCGATTTGTTCG+TGG 0.511021 1.2:+74253015 MS.gene34075:CDS
AGCCGATCTTCACGAGAAGC+GGG 0.511764 1.2:+74254519 MS.gene34075:CDS
AAGCCGATCTTCACGAGAAG+CGG 0.512350 1.2:+74254518 MS.gene34075:CDS
GAAGCGGGAGGTTGCTGTTG+AGG 0.528095 1.2:+74254534 MS.gene34075:CDS
CACCTAACGACCATGGGTTG+CGG 0.528973 1.2:+74251705 None:intergenic
CCCAGTCCCGAGAATGGTGG+AGG 0.543062 1.2:+74251973 MS.gene34075:CDS
GGTCGACCCAGTCCCGAGAA+TGG 0.543531 1.2:+74251967 MS.gene34075:CDS
TTTCAGCAAAGCACAGTACT+GGG 0.546830 1.2:+74253530 MS.gene34075:CDS
CTCCCGCTTCTCGTGAAGAT+CGG 0.553158 1.2:-74254521 None:intergenic
TTGTAATGTAAGGTAACTGT+TGG 0.553827 1.2:+74253198 MS.gene34075:intron
CAACACCAGAATCACCTAAG+AGG 0.554983 1.2:-74252981 None:intergenic
TGTTTCCGTGAAGGATGGCA+TGG 0.556519 1.2:+74254633 MS.gene34075:intron
AAAGGAGGAGCCGCAACCCA+TGG 0.575169 1.2:-74251715 None:intergenic
TTCTGGGCTTGTTATATCGT+AGG 0.577675 1.2:-74253505 None:intergenic
GATAGGGATTCAAGGCAGCT+AGG 0.578929 1.2:+74251946 MS.gene34075:intron
CAGGCAGCAAGAACATCATA+GGG 0.585325 1.2:-74255251 None:intergenic
CGACCCAGTCCCGAGAATGG+TGG 0.607857 1.2:+74251970 MS.gene34075:CDS
GATATAGTGATGGCATTGGT+TGG 0.609417 1.2:+74254490 MS.gene34075:CDS
CCCTGATATAGTGATGGCAT+TGG 0.610235 1.2:+74254486 MS.gene34075:CDS
TGGAAGCCCTGATATAGTGA+TGG 0.626540 1.2:+74254480 MS.gene34075:CDS
ATGGACTATGCAGAGAAGAA+CGG 0.630459 1.2:+74254652 MS.gene34075:CDS
CCAATGCCATCACTATATCA+GGG 0.632375 1.2:-74254486 None:intergenic
TTTGAAATATGGGATACCGC+TGG 0.632905 1.2:+74253273 MS.gene34075:CDS
TCAGTTTGATCCAACATCCA+AGG 0.640164 1.2:+74253038 MS.gene34075:CDS
GGGATACCGCTGGTCAAGAG+AGG 0.645477 1.2:+74253283 MS.gene34075:CDS
ACTGTATTATCGTGGTGCAG+CGG 0.654941 1.2:+74253470 MS.gene34075:CDS
AGAGACATCCGCAAAGACAG+CGG 0.663905 1.2:+74254687 MS.gene34075:CDS
CGATCTTCACGAGAAGCGGG+AGG 0.679341 1.2:+74254522 MS.gene34075:CDS
AAGTTACCTCTCTTGACCAG+CGG 0.701831 1.2:-74253289 None:intergenic
TTGGCACCACTGTATTATCG+TGG 0.723087 1.2:+74253462 MS.gene34075:CDS
GCTGCACCACGATAATACAG+TGG 0.758366 1.2:-74253468 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATTAAAATTATTTGAAT+AGG - chr1.2:74252233-74252252 None:intergenic 10.0%
!! CATATATTCTATAAAATATA+TGG - chr1.2:74252765-74252784 None:intergenic 10.0%
!! TCATTAAAATTATTTGAATA+GGG - chr1.2:74252232-74252251 None:intergenic 10.0%
!! TGAAATATATGTAAATATTT+AGG + chr1.2:74254444-74254463 MS.gene34075:intron 10.0%
!! AGAAGAAATTATTGAATTTA+GGG + chr1.2:74251884-74251903 MS.gene34075:intron 15.0%
!! AGATTAAACTAAAATATTTG+AGG - chr1.2:74252913-74252932 None:intergenic 15.0%
!! TACAATGATTATAAATGAAA+TGG - chr1.2:74253673-74253692 None:intergenic 15.0%
!! TAGAAGAAATTATTGAATTT+AGG + chr1.2:74251883-74251902 MS.gene34075:intron 15.0%
!! TTAAAATTGAATAACTGAAA+GGG - chr1.2:74254755-74254774 None:intergenic 15.0%
!! TTATCAAAAGAATAGAAAAT+AGG + chr1.2:74254285-74254304 MS.gene34075:intron 15.0%
!!! ATTTTGATTTTTATTGAAGT+TGG + chr1.2:74254966-74254985 MS.gene34075:intron 15.0%
!!! CTATATTTTAATTTCTTGTT+TGG + chr1.2:74253583-74253602 MS.gene34075:intron 15.0%
!!! GTTTTCAAAATTAAAGAATT+TGG - chr1.2:74252041-74252060 None:intergenic 15.0%
!!! TGATTTTTTTTTTCATTGTA+TGG + chr1.2:74251813-74251832 MS.gene34075:intron 15.0%
!!! TTTAAAATTGAATAACTGAA+AGG - chr1.2:74254756-74254775 None:intergenic 15.0%
!!! TTTTACAAAAGACTAATTAT+GGG - chr1.2:74252712-74252731 None:intergenic 15.0%
!!! TTTTGATTTTTATTGAAGTT+GGG + chr1.2:74254967-74254986 MS.gene34075:intron 15.0%
!! ATAATAGCCATTAAAAATGA+AGG + chr1.2:74252850-74252869 MS.gene34075:intron 20.0%
!! GTAGATAGTTATACTATTTA+AGG - chr1.2:74254571-74254590 None:intergenic 20.0%
!! TAATAGCCATTAAAAATGAA+GGG + chr1.2:74252851-74252870 MS.gene34075:intron 20.0%
!! TACATCTTGAAATAAGAAAA+GGG - chr1.2:74253392-74253411 None:intergenic 20.0%
!! TATTATTGGGAATTAATTTC+TGG - chr1.2:74252836-74252855 None:intergenic 20.0%
!!! AAAAGTTGAAAAATTTTACC+TGG - chr1.2:74251741-74251760 None:intergenic 20.0%
!!! AATTAGTCTTTTGTAAAACT+TGG + chr1.2:74252714-74252733 MS.gene34075:intron 20.0%
!!! ATATGATTTTGATTTGAAGT+CGG + chr1.2:74252780-74252799 MS.gene34075:intron 20.0%
!!! ATTTTGTATTTGATGAGAAT+GGG + chr1.2:74254787-74254806 MS.gene34075:intron 20.0%
!!! CATTTTTAATGGCTATTATT+GGG - chr1.2:74252849-74252868 None:intergenic 20.0%
!!! GATTATTTTCTTTTTTTCAC+AGG - chr1.2:74254942-74254961 None:intergenic 20.0%
!!! GTAAATTTTCCTAATATTGA+AGG + chr1.2:74253096-74253115 MS.gene34075:intron 20.0%
!!! GTTTTACAAAAGACTAATTA+TGG - chr1.2:74252713-74252732 None:intergenic 20.0%
!!! TATCAATTTTCTTTCTCTAT+GGG + chr1.2:74252276-74252295 MS.gene34075:intron 20.0%
!!! TCATTTTTAATGGCTATTAT+TGG - chr1.2:74252850-74252869 None:intergenic 20.0%
!!! TTTCTATTTGCTGTTAATTT+AGG + chr1.2:74252141-74252160 MS.gene34075:intron 20.0%
!!! TTTTTAGACTTTGTAATGTA+AGG + chr1.2:74253188-74253207 MS.gene34075:intron 20.0%
! AAAATTATACCAGACCTTAT+AGG - chr1.2:74252511-74252530 None:intergenic 25.0%
! AAATTATAAGAACTCCAACA+TGG - chr1.2:74253171-74253190 None:intergenic 25.0%
! AAGTGAATGCATAATTACAT+TGG - chr1.2:74252812-74252831 None:intergenic 25.0%
! ACATCTTGAAATAAGAAAAG+GGG - chr1.2:74253391-74253410 None:intergenic 25.0%
! ATATGGTAATTATTCCATGT+TGG + chr1.2:74253154-74253173 MS.gene34075:intron 25.0%
! ATGTTATACTTTCTATGCTA+TGG + chr1.2:74252576-74252595 MS.gene34075:intron 25.0%
! CTTCACTGTGATTAATAAAA+CGG - chr1.2:74254081-74254100 None:intergenic 25.0%
! GATTTAATGCTTACTAGATA+TGG + chr1.2:74253137-74253156 MS.gene34075:intron 25.0%
! GTACATCTTGAAATAAGAAA+AGG - chr1.2:74253393-74253412 None:intergenic 25.0%
! TAACCAATTCCTTCAATATT+AGG - chr1.2:74253108-74253127 None:intergenic 25.0%
! TAATATAAACGAACTGTTTG+AGG + chr1.2:74254711-74254730 MS.gene34075:CDS 25.0%
! TACATATTATACCCATTAAG+AGG - chr1.2:74253758-74253777 None:intergenic 25.0%
! TAGAGAAAGAAAATTGATAC+AGG - chr1.2:74252275-74252294 None:intergenic 25.0%
! TATAATATGTAGAGAAAAGG+TGG + chr1.2:74253767-74253786 MS.gene34075:intron 25.0%
! TATCTTGTAATGAGAATGAT+TGG - chr1.2:74254251-74254270 None:intergenic 25.0%
! TTCACTGTGATTAATAAAAC+GGG - chr1.2:74254080-74254099 None:intergenic 25.0%
! TTTCCTAATATTGAAGGAAT+TGG + chr1.2:74253102-74253121 MS.gene34075:intron 25.0%
!! AACAAAAAGCATACTATATC+AGG - chr1.2:74254617-74254636 None:intergenic 25.0%
!! AACAAAACCATGTTTAAAAC+AGG - chr1.2:74252099-74252118 None:intergenic 25.0%
!! AACAGTCAAGTTTGAAATAT+GGG + chr1.2:74253263-74253282 MS.gene34075:CDS 25.0%
!! ATTTTTATTGAAGTTGGGAA+TGG + chr1.2:74254972-74254991 MS.gene34075:intron 25.0%
!! CTAAAATATTTGAGGTATGA+AGG - chr1.2:74252905-74252924 None:intergenic 25.0%
!! GATTTTGTATTTGATGAGAA+TGG + chr1.2:74254786-74254805 MS.gene34075:intron 25.0%
!! GTATCAATTTTCTTTCTCTA+TGG + chr1.2:74252275-74252294 MS.gene34075:intron 25.0%
!! GTTTAAAGCACAGTTTATTT+CGG + chr1.2:74252421-74252440 MS.gene34075:intron 25.0%
!! TGATTTTGTGTTTTCAGATA+GGG + chr1.2:74251930-74251949 MS.gene34075:intron 25.0%
!! TTATTGAATTTAGGGTTTGA+CGG + chr1.2:74251892-74251911 MS.gene34075:intron 25.0%
!! TTGAAAAATTTTACCTGGAA+AGG - chr1.2:74251736-74251755 None:intergenic 25.0%
!! TTGATTTTGTGTTTTCAGAT+AGG + chr1.2:74251929-74251948 MS.gene34075:intron 25.0%
!! TTTTGACATTTAGCTGAAAA+GGG + chr1.2:74251776-74251795 MS.gene34075:intron 25.0%
!!! AACAAAATTTGTTTTTCACG+AGG - chr1.2:74253951-74253970 None:intergenic 25.0%
!!! ATTATTGACTTACAGATCTT+CGG + chr1.2:74255134-74255153 MS.gene34075:intron 25.0%
!!! CTAAATCCCTTCATTTTTAA+TGG - chr1.2:74252860-74252879 None:intergenic 25.0%
!!! CTGTTTTTAACTTTTGTCTT+TGG + chr1.2:74255190-74255209 MS.gene34075:intron 25.0%
!!! TAGTAGTTATGTTAGTTTTC+AGG - chr1.2:74254904-74254923 None:intergenic 25.0%
!!! TGAGTAACTTTTTTACAAGA+TGG + chr1.2:74252351-74252370 MS.gene34075:intron 25.0%
!!! TGTTATACCTGTTTTAAACA+TGG + chr1.2:74252089-74252108 MS.gene34075:intron 25.0%
!!! TTTTAGTTAACTGTCCTATA+AGG + chr1.2:74252494-74252513 MS.gene34075:intron 25.0%
AACAAAATAAGCGCTTGTAA+TGG - chr1.2:74253355-74253374 None:intergenic 30.0%
AATAATTCGCTTCATTCCAA+AGG - chr1.2:74252460-74252479 None:intergenic 30.0%
ACAGGAAAACCAAGAATAAA+TGG + chr1.2:74253861-74253880 MS.gene34075:intron 30.0%
ACATTGAGTTCTATCAAACA+TGG - chr1.2:74253901-74253920 None:intergenic 30.0%
ATTATACCCATTAAGAGGTA+AGG - chr1.2:74253753-74253772 None:intergenic 30.0%
ATTGAGTTCTATCAAACATG+GGG - chr1.2:74253899-74253918 None:intergenic 30.0%
ATTTAGGAGCTACAAAAACA+TGG + chr1.2:74254460-74254479 MS.gene34075:intron 30.0%
CATCTTGAAATAAGAAAAGG+GGG - chr1.2:74253390-74253409 None:intergenic 30.0%
CATTGAGTTCTATCAAACAT+GGG - chr1.2:74253900-74253919 None:intergenic 30.0%
CTACAGAGATTGAAATAAAC+CGG + chr1.2:74254159-74254178 MS.gene34075:intron 30.0%
GAATAGCTCAAAATTTGTAC+AGG + chr1.2:74253843-74253862 MS.gene34075:intron 30.0%
GGGTATAATATGTAGAGAAA+AGG + chr1.2:74253764-74253783 MS.gene34075:intron 30.0%
GGTTTATTTCAATCTCTGTA+GGG - chr1.2:74254160-74254179 None:intergenic 30.0%
GTTTATTTCAATCTCTGTAG+GGG - chr1.2:74254159-74254178 None:intergenic 30.0%
TACAAGATGGTAAAAGACAA+AGG + chr1.2:74252364-74252383 MS.gene34075:intron 30.0%
TACAGAGATTGAAATAAACC+GGG + chr1.2:74254160-74254179 MS.gene34075:intron 30.0%
TAGAAAATAATCCCTTAGCT+TGG + chr1.2:74254823-74254842 MS.gene34075:intron 30.0%
TATGATCAACTCTTCTTACT+GGG - chr1.2:74253823-74253842 None:intergenic 30.0%
TATGTTGTATTCACTGATGT+TGG - chr1.2:74253638-74253657 None:intergenic 30.0%
TTATACCCATTAAGAGGTAA+GGG - chr1.2:74253752-74253771 None:intergenic 30.0%
TTGTAATGTAAGGTAACTGT+TGG + chr1.2:74253198-74253217 MS.gene34075:intron 30.0%
TTTGACATTTAGCTGAAAAG+GGG + chr1.2:74251777-74251796 MS.gene34075:intron 30.0%
! AAAAATTTTACCTGGAAAGG+AGG - chr1.2:74251733-74251752 None:intergenic 30.0%
! CAACAGTCAAGTTTGAAATA+TGG + chr1.2:74253262-74253281 MS.gene34075:CDS 30.0%
! CTTTTGACATTTAGCTGAAA+AGG + chr1.2:74251775-74251794 MS.gene34075:intron 30.0%
! TAATTAGTTCAGCTTTTGCT+TGG + chr1.2:74252325-74252344 MS.gene34075:intron 30.0%
! TATAAGGTGCCAAAATGATA+TGG + chr1.2:74255056-74255075 MS.gene34075:intron 30.0%
! TATTGAATTTAGGGTTTGAC+GGG + chr1.2:74251893-74251912 MS.gene34075:intron 30.0%
! TGTGATTAATAAAACGGGTT+TGG - chr1.2:74254075-74254094 None:intergenic 30.0%
!! AAAATTTGTTTTTCACGAGG+TGG - chr1.2:74253948-74253967 None:intergenic 30.0%
!! CTATTTGCTGTTAATTTAGG+AGG + chr1.2:74252144-74252163 MS.gene34075:intron 30.0%
!! TGAACCATGTTTTGAGTATA+AGG + chr1.2:74255040-74255059 MS.gene34075:intron 30.0%
!! TTATTGACTTACAGATCTTC+GGG + chr1.2:74255135-74255154 MS.gene34075:intron 30.0%
!!! ATTTTGGGTTTGTGGTTTAA+TGG + chr1.2:74251848-74251867 MS.gene34075:intron 30.0%
!!! TTTTGGGTTTGTGGTTTAAT+GGG + chr1.2:74251849-74251868 MS.gene34075:intron 30.0%
AAAGCCACATATTCTTACCT+TGG - chr1.2:74253058-74253077 None:intergenic 35.0%
ACATCCAAGGTAAGAATATG+TGG + chr1.2:74253051-74253070 MS.gene34075:intron 35.0%
ATAAAACCTGCATCTTAGGT+GGG + chr1.2:74254003-74254022 MS.gene34075:intron 35.0%
ATGATCAACTCTTCTTACTG+GGG - chr1.2:74253822-74253841 None:intergenic 35.0%
CATACCTGAAACAGATGATT+GGG - chr1.2:74253437-74253456 None:intergenic 35.0%
CATATTCTTACCTTGGATGT+TGG - chr1.2:74253051-74253070 None:intergenic 35.0%
CGCTAAAAATCTTCGTGTTA+AGG + chr1.2:74252002-74252021 MS.gene34075:CDS 35.0%
CGGTTTATTCTTTGTTCCTT+TGG + chr1.2:74252441-74252460 MS.gene34075:intron 35.0%
CGGTTTATTTCAATCTCTGT+AGG - chr1.2:74254161-74254180 None:intergenic 35.0%
CTATGATCAACTCTTCTTAC+TGG - chr1.2:74253824-74253843 None:intergenic 35.0%
CTGTGTTCAGTGACATTTAA+AGG - chr1.2:74252956-74252975 None:intergenic 35.0%
GAAAGGGATGTAAACATCAA+AGG - chr1.2:74254739-74254758 None:intergenic 35.0%
GCACGTGAGATTTCATTTAT+TGG + chr1.2:74254202-74254221 MS.gene34075:intron 35.0%
GTTCATAAAACCTGCATCTT+AGG + chr1.2:74253999-74254018 MS.gene34075:intron 35.0%
TATATTATCCGCTGTCTTTG+CGG - chr1.2:74254698-74254717 None:intergenic 35.0%
TATTGAAGTTGGGAATGGAA+TGG + chr1.2:74254977-74254996 MS.gene34075:intron 35.0%
TCTCGAAAATATGCACAACA+TGG + chr1.2:74252737-74252756 MS.gene34075:intron 35.0%
TCTTGCTTAAAACACCTTCA+GGG - chr1.2:74254865-74254884 None:intergenic 35.0%
TTCTTGCTTAAAACACCTTC+AGG - chr1.2:74254866-74254885 None:intergenic 35.0%
TTGACAAATGACTGTAAGAG+TGG - chr1.2:74252620-74252639 None:intergenic 35.0%
! AAAACCGGACCATATCATTT+TGG - chr1.2:74255068-74255087 None:intergenic 35.0%
! AAATAATCCCTTAGCTTGGT+TGG + chr1.2:74254827-74254846 MS.gene34075:intron 35.0%
! ACTGTGCTTTAAACTTTCAG+TGG - chr1.2:74252415-74252434 None:intergenic 35.0%
! ACTTTTGTCTTTGGTCTCTT+AGG + chr1.2:74255199-74255218 MS.gene34075:intron 35.0%
! ATTGAATTTAGGGTTTGACG+GGG + chr1.2:74251894-74251913 MS.gene34075:intron 35.0%
! TGTTTTCAGATAGGGATTCA+AGG + chr1.2:74251938-74251957 MS.gene34075:intron 35.0%
! TTGTTGCAGATGGTTCATTT+TGG + chr1.2:74252194-74252213 MS.gene34075:intron 35.0%
! TTTGGGTTTGTGGTTTAATG+GGG + chr1.2:74251850-74251869 MS.gene34075:intron 35.0%
! TTTTTGATGTACTCCTTCTG+AGG - chr1.2:74252657-74252676 None:intergenic 35.0%
!! AGGAGGTAGTTTGTTGTAAT+AGG + chr1.2:74252161-74252180 MS.gene34075:intron 35.0%
!! AGTTTTCAGGATTTGAACTC+TGG - chr1.2:74254891-74254910 None:intergenic 35.0%
!! ATGGCTTCGATTTGTGATTT+TGG + chr1.2:74251832-74251851 MS.gene34075:intron 35.0%
!! TATGCTTTTTGTTTCCGTGA+AGG + chr1.2:74254624-74254643 MS.gene34075:intron 35.0%
!! TGGCTTCGATTTGTGATTTT+GGG + chr1.2:74251833-74251852 MS.gene34075:intron 35.0%
!!! GATTTGTGATTTTGGGTTTG+TGG + chr1.2:74251840-74251859 MS.gene34075:intron 35.0%
!!! TGCATGTTTTTTGTTGCAGA+TGG + chr1.2:74252184-74252203 MS.gene34075:intron 35.0%
AATGAAATCTCACGTGCGTA+AGG - chr1.2:74254199-74254218 None:intergenic 40.0%
ACAACACCCTTACCTCTTAA+TGG + chr1.2:74253743-74253762 MS.gene34075:intron 40.0%
ACATTTAGCTGAAAAGGGGT+TGG + chr1.2:74251781-74251800 MS.gene34075:intron 40.0%
ACCAATGCCATCACTATATC+AGG - chr1.2:74254490-74254509 None:intergenic 40.0%
AGAATGAATGATGCGCAATC+TGG - chr1.2:74253327-74253346 None:intergenic 40.0%
CAACACCCTTACCTCTTAAT+GGG + chr1.2:74253744-74253763 MS.gene34075:intron 40.0%
CACATGATCAGCATCAAAAC+CGG - chr1.2:74255083-74255102 None:intergenic 40.0%
CACGAGGATACATGTTTATC+TGG - chr1.2:74253715-74253734 None:intergenic 40.0%
CATAAAACCTGCATCTTAGG+TGG + chr1.2:74254002-74254021 MS.gene34075:intron 40.0%
CCAATGCCATCACTATATCA+GGG - chr1.2:74254489-74254508 None:intergenic 40.0%
GATTCCCAATCATCTGTTTC+AGG + chr1.2:74253430-74253449 MS.gene34075:intron 40.0%
GCATACCTGAAACAGATGAT+TGG - chr1.2:74253438-74253457 None:intergenic 40.0%
GGCACCTTATACTCAAAACA+TGG - chr1.2:74255047-74255066 None:intergenic 40.0%
GTGCTTTGCTGAAAGATTCT+GGG - chr1.2:74253524-74253543 None:intergenic 40.0%
GTTAACTGTCCTATAAGGTC+TGG + chr1.2:74252499-74252518 MS.gene34075:intron 40.0%
GTTTGTGGTTTAATGGGGTT+TGG + chr1.2:74251855-74251874 MS.gene34075:intron 40.0%
TAGTTCACCAACCAAGCTAA+GGG - chr1.2:74254837-74254856 None:intergenic 40.0%
TCAGTTTGATCCAACATCCA+AGG + chr1.2:74253038-74253057 MS.gene34075:CDS 40.0%
TCATCATTGAGATGCTGACA+TGG - chr1.2:74252680-74252699 None:intergenic 40.0%
TGATCATGTGGTATGAAGCT+TGG + chr1.2:74255093-74255112 MS.gene34075:intron 40.0%
TGCACAGAACAGGCAAATAT+TGG - chr1.2:74254109-74254128 None:intergenic 40.0%
TGTGCTTTGCTGAAAGATTC+TGG - chr1.2:74253525-74253544 None:intergenic 40.0%
TTAGTTCACCAACCAAGCTA+AGG - chr1.2:74254838-74254857 None:intergenic 40.0%
TTTCAGCAAAGCACAGTACT+GGG + chr1.2:74253530-74253549 MS.gene34075:CDS 40.0%
! AACGTAAAAGTGCACAGAAC+AGG - chr1.2:74254119-74254138 None:intergenic 40.0%
! ATGGACTATGCAGAGAAGAA+CGG + chr1.2:74254652-74254671 MS.gene34075:CDS 40.0%
! ATTGTTCTGCGATTTGTTCG+TGG + chr1.2:74253015-74253034 MS.gene34075:CDS 40.0%
! TTTGAAATATGGGATACCGC+TGG + chr1.2:74253273-74253292 MS.gene34075:CDS 40.0%
!! CTTTTTGTTTCCGTGAAGGA+TGG + chr1.2:74254628-74254647 MS.gene34075:intron 40.0%
!! GATATAGTGATGGCATTGGT+TGG + chr1.2:74254490-74254509 MS.gene34075:CDS 40.0%
!! TTCTGGGCTTGTTATATCGT+AGG - chr1.2:74253508-74253527 None:intergenic 40.0%
AAGAATAAATGGCCTCTCCG+AGG + chr1.2:74253872-74253891 MS.gene34075:intron 45.0%
AAGTTACCTCTCTTGACCAG+CGG - chr1.2:74253292-74253311 None:intergenic 45.0%
ACTGTATTATCGTGGTGCAG+CGG + chr1.2:74253470-74253489 MS.gene34075:CDS 45.0%
AGATCTTCGGGACGTTTCAA+AGG + chr1.2:74255147-74255166 MS.gene34075:intron 45.0%
ATAGTCCATGCCATCCTTCA+CGG - chr1.2:74254641-74254660 None:intergenic 45.0%
CAACACCAGAATCACCTAAG+AGG - chr1.2:74252984-74253003 None:intergenic 45.0%
CAGGCAGCAAGAACATCATA+GGG - chr1.2:74255254-74255273 None:intergenic 45.0%
CCCTGATATAGTGATGGCAT+TGG + chr1.2:74254486-74254505 MS.gene34075:CDS 45.0%
CTCTTAGGTGATTCTGGTGT+TGG + chr1.2:74252982-74253001 MS.gene34075:CDS 45.0%
CTTATTCCCACCTAAGATGC+AGG - chr1.2:74254012-74254031 None:intergenic 45.0%
CTTCTTACTGGGGCTGATAT+TGG - chr1.2:74253812-74253831 None:intergenic 45.0%
CTTGTCCTCTTAGGTGATTC+TGG + chr1.2:74252976-74252995 MS.gene34075:CDS 45.0%
CTTTCAGCAAAGCACAGTAC+TGG + chr1.2:74253529-74253548 MS.gene34075:CDS 45.0%
GGTGCCAAAATGATATGGTC+CGG + chr1.2:74255061-74255080 MS.gene34075:intron 45.0%
TAAGGAGTAACGAAGGAACC+CGG - chr1.2:74254181-74254200 None:intergenic 45.0%
TCAGGCAGCAAGAACATCAT+AGG - chr1.2:74255255-74255274 None:intergenic 45.0%
TCGGAGAGGCCATTTATTCT+TGG - chr1.2:74253873-74253892 None:intergenic 45.0%
TCTATCAAACATGGGGTCCT+CGG - chr1.2:74253892-74253911 None:intergenic 45.0%
TGAACACAGCTTGTCCTCTT+AGG + chr1.2:74252967-74252986 MS.gene34075:intron 45.0%
TGGAAGCCCTGATATAGTGA+TGG + chr1.2:74254480-74254499 MS.gene34075:CDS 45.0%
TGTGGTATGAAGCTTGGTCT+TGG + chr1.2:74255099-74255118 MS.gene34075:intron 45.0%
TTGGCACCACTGTATTATCG+TGG + chr1.2:74253462-74253481 MS.gene34075:CDS 45.0%
! CAAAGCACAGTACTGGGTTA+AGG + chr1.2:74253536-74253555 MS.gene34075:CDS 45.0%
! CTGTTTCAGGTATGCTGCAT+TGG + chr1.2:74253443-74253462 MS.gene34075:intron 45.0%
! GGTGTTGTCATTAAGTCACG+AGG - chr1.2:74253731-74253750 None:intergenic 45.0%
! GTGGTATGAAGCTTGGTCTT+GGG + chr1.2:74255100-74255119 MS.gene34075:intron 45.0%
! TTAGGGTTTGACGGGGTTTT+TGG + chr1.2:74251901-74251920 MS.gene34075:intron 45.0%
!! CGGTTTTGATGCTGATCATG+TGG + chr1.2:74255081-74255100 MS.gene34075:intron 45.0%
!!! ATTCAATTTTAAATTAATTA+TGG + chr1.2:74254763-74254782 MS.gene34075:intron 5.0%
!!! TTCAATTTTAAATTAATTAT+GGG + chr1.2:74254764-74254783 MS.gene34075:intron 5.0%
AAGCCGATCTTCACGAGAAG+CGG + chr1.2:74254518-74254537 MS.gene34075:CDS 50.0%
ACGTGCGTAAGGAGTAACGA+AGG - chr1.2:74254188-74254207 None:intergenic 50.0%
AGAGACATCCGCAAAGACAG+CGG + chr1.2:74254687-74254706 MS.gene34075:CDS 50.0%
CCTGCAAACACATCCTCAGA+AGG + chr1.2:74252641-74252660 MS.gene34075:intron 50.0%
GATAGGGATTCAAGGCAGCT+AGG + chr1.2:74251946-74251965 MS.gene34075:intron 50.0%
GCTGCACCACGATAATACAG+TGG - chr1.2:74253471-74253490 None:intergenic 50.0%
GCTTACGCATGTGTGAGCAA+TGG - chr1.2:74254363-74254382 None:intergenic 50.0%
GGTGAACTAAGTGACCCTGA+AGG + chr1.2:74254848-74254867 MS.gene34075:intron 50.0%
GTCTTGTCCTCCACCATTCT+CGG - chr1.2:74251983-74252002 None:intergenic 50.0%
TCTTGTCCTCCACCATTCTC+GGG - chr1.2:74251982-74252001 None:intergenic 50.0%
TGTTTCCGTGAAGGATGGCA+TGG + chr1.2:74254633-74254652 MS.gene34075:intron 50.0%
! CCTTCTGAGGATGTGTTTGC+AGG - chr1.2:74252644-74252663 None:intergenic 50.0%
!! GGGGCTGATATTGGTCTTCT+AGG - chr1.2:74253803-74253822 None:intergenic 50.0%
AGCCGATCTTCACGAGAAGC+GGG + chr1.2:74254519-74254538 MS.gene34075:CDS 55.0%
CTCCCGCTTCTCGTGAAGAT+CGG - chr1.2:74254524-74254543 None:intergenic 55.0%
TCGGGACGTTTCAAAGGCTG+TGG + chr1.2:74255153-74255172 MS.gene34075:intron 55.0%
CAAACATGGGGTCCTCGGAG+AGG - chr1.2:74253887-74253906 None:intergenic 60.0%
CCTCCACCATTCTCGGGACT+GGG - chr1.2:74251976-74251995 None:intergenic 60.0%
CGATCTTCACGAGAAGCGGG+AGG + chr1.2:74254522-74254541 MS.gene34075:CDS 60.0%
GGGATACCGCTGGTCAAGAG+AGG + chr1.2:74253283-74253302 MS.gene34075:CDS 60.0%
TCCTCCACCATTCTCGGGAC+TGG - chr1.2:74251977-74251996 None:intergenic 60.0%
! GAAGCGGGAGGTTGCTGTTG+AGG + chr1.2:74254534-74254553 MS.gene34075:CDS 60.0%
CCCAGTCCCGAGAATGGTGG+AGG + chr1.2:74251973-74251992 MS.gene34075:CDS 65.0%
CGACCCAGTCCCGAGAATGG+TGG + chr1.2:74251970-74251989 MS.gene34075:CDS 65.0%
GGTCGACCCAGTCCCGAGAA+TGG + chr1.2:74251967-74251986 MS.gene34075:CDS 65.0%
GGTTGCGGCTCCTCCTTTCC+AGG + chr1.2:74251720-74251739 MS.gene34075:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 74251717 74255274 74251717 ID=MS.gene34075
chr1.2 mRNA 74251717 74255274 74251717 ID=MS.gene34075.t1;Parent=MS.gene34075
chr1.2 exon 74251717 74251741 74251717 ID=MS.gene34075.t1.exon1;Parent=MS.gene34075.t1
chr1.2 CDS 74251717 74251741 74251717 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74251947 74252023 74251947 ID=MS.gene34075.t1.exon2;Parent=MS.gene34075.t1
chr1.2 CDS 74251947 74252023 74251947 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74252976 74253059 74252976 ID=MS.gene34075.t1.exon3;Parent=MS.gene34075.t1
chr1.2 CDS 74252976 74253059 74252976 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74253210 74253304 74253210 ID=MS.gene34075.t1.exon4;Parent=MS.gene34075.t1
chr1.2 CDS 74253210 74253304 74253210 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74253452 74253557 74253452 ID=MS.gene34075.t1.exon5;Parent=MS.gene34075.t1
chr1.2 CDS 74253452 74253557 74253452 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74254466 74254555 74254466 ID=MS.gene34075.t1.exon6;Parent=MS.gene34075.t1
chr1.2 CDS 74254466 74254555 74254466 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74254646 74254732 74254646 ID=MS.gene34075.t1.exon7;Parent=MS.gene34075.t1
chr1.2 CDS 74254646 74254732 74254646 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
chr1.2 exon 74255221 74255274 74255221 ID=MS.gene34075.t1.exon8;Parent=MS.gene34075.t1
chr1.2 CDS 74255221 74255274 74255221 ID=cds.MS.gene34075.t1;Parent=MS.gene34075.t1
Gene Sequence

>MS.gene34075

ATGGGTTGCGGCTCCTCCTTTCCAGGTAAAATTTTTCAACTTTTCTTCAAATTCTCATCTTTTGACATTTAGCTGAAAAGGGGTTGGAAAAAAAATTGATTTTTTTTTTCATTGTATGGCTTCGATTTGTGATTTTGGGTTTGTGGTTTAATGGGGTTTGGTTAATTAGAAGAAATTATTGAATTTAGGGTTTGACGGGGTTTTTGGTTAATTTGATTTTGTGTTTTCAGATAGGGATTCAAGGCAGCTAGGTCGACCCAGTCCCGAGAATGGTGGAGGACAAGACGCTAAAAATCTTCGTGTTAAGGTATTCATTTTATGCCAAATTCTTTAATTTTGAAAACATGTTTTTTGTTTTGTTGTTGTTGTTGTTGTTATACCTGTTTTAAACATGGTTTTGTTCTGTTTCATGTTTCTTGTAGATTTTCTATTTGCTGTTAATTTAGGAGGTAGTTTGTTGTAATAGGTGCATGTTTTTTGTTGCAGATGGTTCATTTTGGATTTTTGTTTCACCCTATTCAAATAATTTTAATGATTGTGATTCGATGCTCAAATCCTGTATCAATTTTCTTTCTCTATGGGAGACTTTGTATTTATGCAGTAAATAGTAATTAGTTCAGCTTTTGCTTGGATTTGAGTAACTTTTTTACAAGATGGTAAAAGACAAAGGAAGTAATACAATTTTATTAAAATAACCACTGAAAGTTTAAAGCACAGTTTATTTCGGTTTATTCTTTGTTCCTTTGGAATGAAGCGAATTATTTTGTACACTTTTTCTTTTAGTTAACTGTCCTATAAGGTCTGGTATAATTTTTTGTTATGAGTTAGTAAAAGAATCACATCGAAGTTTCTGTGTCACATGTTATACTTTCTATGCTATGGCAAAGAAATAACTATCAACCACTCTTACAGTCATTTGTCAAACCTGCAAACACATCCTCAGAAGGAGTACATCAAAAACCATGTCAGCATCTCAATGATGACAATTTTCACCCATAATTAGTCTTTTGTAAAACTTGGTCTCGAAAATATGCACAACATGGAGCCATATATTTTATAGAATATATGATTTTGATTTGAAGTCGGATGTTGCCAATGTAATTATGCATTCACTTGCCAGAAATTAATTCCCAATAATAGCCATTAAAAATGAAGGGATTTAGTTGAAATTATTAATAAAAAATTCCTTCATACCTCAAATATTTTAGTTTAATCTTTGAATGACTCAATAACTAACCTTTAAATGTCACTGAACACAGCTTGTCCTCTTAGGTGATTCTGGTGTTGGTAAGAGCTGCATTGTTCTGCGATTTGTTCGTGGTCAGTTTGATCCAACATCCAAGGTAAGAATATGTGGCTTTTATCATTTCTTTTTTAGTGTAAATTTTCCTAATATTGAAGGAATTGGTTATATGAAGTAGATTTAATGCTTACTAGATATGGTAATTATTCCATGTTGGAGTTCTTATAATTTTTAGACTTTGTAATGTAAGGTAACTGTTGGAGCTTCTTTCTTGTCACAAACAATCGCTCTGCAAGACTCTACAACAGTCAAGTTTGAAATATGGGATACCGCTGGTCAAGAGAGGTAACTTTCATTTATCTTTCCAGATTGCGCATCATTCATTCTACAAACCATTACAAGCGCTTATTTTGTTTCTCATAATATACCCCCTTTTCTTATTTCAAGATGTACATTCTGAAGTAAAATTTGATTCCCAATCATCTGTTTCAGGTATGCTGCATTGGCACCACTGTATTATCGTGGTGCAGCGGTTGCAGTTATTGCCTACGATATAACAAGCCCAGAATCTTTCAGCAAAGCACAGTACTGGGTTAAGGTATATTTGCAGTCATCACATGCTTCTATATTTTAATTTCTTGTTTGGTATAGTTATTACTTGAATGATGTGATTAACCAACATCAGTGAATACAACATAATCAGTTTTAATCCATTTCATTTATAATCATTGTAGTTTAGTAATTTTAAGTTACCAGATAAACATGTATCCTCGTGACTTAATGACAACACCCTTACCTCTTAATGGGTATAATATGTAGAGAAAAGGTGGTAGAATCATGCCTAGAAGACCAATATCAGCCCCAGTAAGAAGAGTTGATCATAGAATAGCTCAAAATTTGTACAGGAAAACCAAGAATAAATGGCCTCTCCGAGGACCCCATGTTTGATAGAACTCAATGTCGTCGTTTGATTTTGTAGCAGACACCACCTCGTGAAAAACAAATTTTGTTTTTGTTTTTGTTTGTAGAAAGACAAGAAGTTCATAAAACCTGCATCTTAGGTGGGAATAAGTATGTACTTAGTTACTAGTTATTAGAATTAGAATTGTTCACCAAACCCGTTTTATTAATCACAGTGAAGTAGCACCAATATTTGCCTGTTCTGTGCACTTTTACGTTAAAAAATCAACTTTCTGCCCCTACAGAGATTGAAATAAACCGGGTTCCTTCGTTACTCCTTACGCACGTGAGATTTCATTTATTGGTTTCTGTTAGAGTTGAAAGAAATCCAATCATTCTCATTACAAGATAAGTTGTTGAAAACGTTATCAAAAGAATAGAAAATAGGTAATAATTGTCTTTGACAAACTCATGATCAATATCTATCTTTTGATGTATTGCCATTGCTCACACATGCGTAAGCGCGCAGACATGCAGATACATGCATATATTTCTACAATTTCTGTCAGTTTTGTTTTAGCAGCTGAAATATATGTAAATATTTAGGAGCTACAAAAACATGGAAGCCCTGATATAGTGATGGCATTGGTTGGTAACAAAGCCGATCTTCACGAGAAGCGGGAGGTTGCTGTTGAGGTAATGCAACAACCTTAAATAGTATAACTATCTACTAAACAAAACAATGAATTGTTTTCCTGATATAGTATGCTTTTTGTTTCCGTGAAGGATGGCATGGACTATGCAGAGAAGAACGGAATGTTTTTTATAGAGACATCCGCAAAGACAGCGGATAATATAAACGAACTGTTTGAGGTACCTTTGATGTTTACATCCCTTTCAGTTATTCAATTTTAAATTAATTATGGGATTTTGTATTTGATGAGAATGGGAATGAGTGAACAATAGAAAATAATCCCTTAGCTTGGTTGGTGAACTAAGTGACCCTGAAGGTGTTTTAAGCAAGAAGTCCAGAGTTCAAATCCTGAAAACTAACATAACTACTAACTTACTAACATTAACCTGTGAAAAAAAGAAAATAATCTGTTATTTTGATTTTTATTGAAGTTGGGAATGGAATGGACATTCATCACATCTTACGCTTCTAACTCTACAATTGTTTTGAACCATGTTTTGAGTATAAGGTGCCAAAATGATATGGTCCGGTTTTGATGCTGATCATGTGGTATGAAGCTTGGTCTTGGGAAGTTCTGTTTATTATTGACTTACAGATCTTCGGGACGTTTCAAAGGCTGTGGTAGTAGTAAATTTTCTGTTTTTAACTTTTGTCTTTGGTCTCTTAGGACATTGTTGACTATGCTACTAGCAAGCTTCCCTATGATGTTCTTGCTGCCTGA
Protein sequence

>MS.gene34075.t1

MGCGSSFPDRDSRQLGRPSPENGGGQDAKNLRVKLVLLGDSGVGKSCIVLRFVRGQFDPTSKVTVGASFLSQTIALQDSTTVKFEIWDTAGQERYAALAPLYYRGAAVAVIAYDITSPESFSKAQYWVKELQKHGSPDIVMALVGNKADLHEKREVAVEDGMDYAEKNGMFFIETSAKTADNINELFEDIVDYATSKLPYDVLAA