AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000342

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000342.t1 MTR_7g405790 99.545 220 0 1 1 219 1 220 1.58e-160 442
MS.gene000342.t1 MTR_7g051940 80.193 207 38 1 13 219 12 215 7.61e-120 339
MS.gene000342.t1 MTR_1g078200 62.555 227 73 2 1 219 1 223 1.95e-97 283
MS.gene000342.t1 MTR_8g089985 60.914 197 66 1 12 208 15 200 8.39e-85 251
MS.gene000342.t1 MTR_1g052475 55.399 213 86 3 12 218 13 222 6.33e-84 249
MS.gene000342.t1 MTR_4g064897 55.288 208 90 1 12 219 10 214 7.86e-84 248
MS.gene000342.t1 MTR_3g086980 55.660 212 85 2 12 218 14 221 3.33e-83 247
MS.gene000342.t1 MTR_2g075950 65.318 173 60 0 12 184 11 183 5.77e-82 243
MS.gene000342.t1 MTR_2g083860 58.173 208 86 1 12 219 11 217 1.46e-81 243
MS.gene000342.t1 MTR_0262s0050 61.932 176 67 0 12 187 13 188 7.44e-81 241
MS.gene000342.t1 MTR_8g036220 54.378 217 91 2 10 219 12 227 1.33e-80 240
MS.gene000342.t1 MTR_2g005510 60.428 187 73 1 1 186 1 187 3.67e-80 239
MS.gene000342.t1 MTR_4g079350 56.731 208 88 1 12 219 11 216 3.87e-80 239
MS.gene000342.t1 MTR_3g069510 54.327 208 93 1 12 219 10 215 7.78e-80 238
MS.gene000342.t1 MTR_4g088090 56.731 208 88 1 12 219 11 216 9.69e-80 238
MS.gene000342.t1 MTR_2g090865 58.654 208 83 2 12 219 9 213 2.59e-79 236
MS.gene000342.t1 MTR_2g014990 56.731 208 88 1 12 219 11 216 3.88e-79 236
MS.gene000342.t1 MTR_8g027220 51.208 207 100 1 12 217 24 230 1.43e-72 220
MS.gene000342.t1 MTR_8g107070 55.851 188 81 1 12 199 12 197 8.93e-66 204
MS.gene000342.t1 MTR_6g079550 48.558 208 105 1 12 219 10 215 1.90e-65 201
MS.gene000342.t1 MTR_7g117910 54.430 158 72 0 12 169 18 175 2.87e-61 191
MS.gene000342.t1 MTR_2g027660 43.541 209 114 2 13 219 5 211 9.31e-59 184
MS.gene000342.t1 MTR_6g021790 43.810 210 114 2 12 219 4 211 2.21e-57 181
MS.gene000342.t1 MTR_7g081700 48.276 174 90 0 12 185 6 179 1.60e-53 171
MS.gene000342.t1 MTR_8g031870 51.923 156 75 0 13 168 5 160 5.93e-53 169
MS.gene000342.t1 MTR_2g005500 42.927 205 112 1 1 205 1 200 7.24e-53 169
MS.gene000342.t1 MTR_8g031680 42.512 207 118 1 13 219 5 210 1.66e-51 166
MS.gene000342.t1 MTR_8g031660 48.503 167 86 0 13 179 5 171 1.94e-51 166
MS.gene000342.t1 MTR_1g115325 39.904 208 118 3 12 218 13 214 4.44e-51 165
MS.gene000342.t1 MTR_1g068740 40.385 208 117 4 12 218 13 214 2.28e-50 163
MS.gene000342.t1 MTR_1g068730 40.385 208 117 4 12 218 13 214 3.63e-50 162
MS.gene000342.t1 MTR_8g006970 50.955 157 77 0 12 168 6 162 4.01e-50 161
MS.gene000342.t1 MTR_8g006970 50.955 157 77 0 12 168 20 176 5.02e-50 162
MS.gene000342.t1 MTR_7g100020 44.848 165 90 1 12 175 13 177 4.42e-48 157
MS.gene000342.t1 MTR_8g031830 49.020 153 78 0 13 165 5 157 2.43e-47 155
MS.gene000342.t1 MTR_2g096550 36.842 209 121 4 12 218 13 212 4.90e-47 154
MS.gene000342.t1 MTR_1g094220 43.182 176 98 2 2 175 18 193 8.99e-47 153
MS.gene000342.t1 MTR_1g094220 42.162 185 105 2 2 184 18 202 1.14e-46 153
MS.gene000342.t1 MTR_1g094220 43.931 173 95 2 2 172 18 190 2.17e-46 152
MS.gene000342.t1 MTR_1g094220 44.444 171 93 2 2 170 18 188 7.62e-46 152
MS.gene000342.t1 MTR_4g063160 38.498 213 115 6 12 218 13 215 2.36e-44 147
MS.gene000342.t1 MTR_2g102060 36.765 204 113 2 16 219 12 199 4.40e-44 146
MS.gene000342.t1 MTR_3g064390 41.875 160 93 0 16 175 12 171 9.39e-44 145
MS.gene000342.t1 MTR_8g093740 43.860 171 93 3 12 180 11 180 1.39e-42 143
MS.gene000342.t1 MTR_4g099470 46.479 142 76 0 45 186 15 156 6.81e-42 140
MS.gene000342.t1 MTR_5g076590 40.000 160 96 0 16 175 12 171 2.03e-41 139
MS.gene000342.t1 MTR_3g087160 38.854 157 96 0 15 171 10 166 6.15e-41 139
MS.gene000342.t1 MTR_8g090215 34.975 203 127 2 15 217 10 207 8.28e-41 138
MS.gene000342.t1 MTR_7g103070 45.833 168 89 2 15 180 17 184 9.85e-41 138
MS.gene000342.t1 MTR_1g068740 43.056 144 74 1 12 155 13 148 1.88e-40 137
MS.gene000342.t1 MTR_5g013900 39.490 157 95 0 15 171 10 166 2.33e-40 137
MS.gene000342.t1 MTR_1g068730 45.385 130 71 0 12 141 13 142 2.76e-40 136
MS.gene000342.t1 MTR_3g096350 43.275 171 94 3 12 180 10 179 4.18e-40 136
MS.gene000342.t1 MTR_8g103550 39.286 168 101 1 16 182 11 178 2.15e-39 134
MS.gene000342.t1 MTR_1g062760 46.012 163 85 3 15 175 15 176 5.70e-39 134
MS.gene000342.t1 MTR_1g029450 42.105 171 96 3 12 180 15 184 1.14e-38 133
MS.gene000342.t1 MTR_4g098950 37.580 157 98 0 15 171 10 166 3.04e-38 132
MS.gene000342.t1 MTR_8g092020 41.317 167 91 3 14 173 12 178 1.28e-37 130
MS.gene000342.t1 MTR_1g090717 44.444 171 92 3 2 169 3 173 5.28e-37 129
MS.gene000342.t1 MTR_1g097420 39.535 172 97 3 14 178 8 179 1.87e-36 127
MS.gene000342.t1 MTR_2g101890 37.209 172 101 3 14 178 10 181 4.26e-35 124
MS.gene000342.t1 MTR_4g069850 36.310 168 100 3 14 174 8 175 7.11e-35 123
MS.gene000342.t1 MTR_4g012940 36.628 172 102 3 14 178 8 179 3.54e-34 121
MS.gene000342.t1 MTR_3g064390 41.600 125 73 0 16 140 12 136 1.04e-32 116
MS.gene000342.t1 MTR_6g039490 38.372 172 99 3 14 178 8 179 1.46e-32 117
MS.gene000342.t1 MTR_3g096350 46.154 130 67 3 12 139 10 138 2.37e-32 115
MS.gene000342.t1 MTR_3g096350 47.107 121 61 3 12 130 10 129 1.66e-31 113
MS.gene000342.t1 MTR_1g097420 36.994 173 100 4 13 178 36 206 1.60e-30 112
MS.gene000342.t1 MTR_8g093740 42.029 138 78 2 45 180 10 147 1.68e-30 111
MS.gene000342.t1 MTR_7g103070 46.457 127 66 2 56 180 2 128 3.05e-29 107
MS.gene000342.t1 MTR_2g005500 30.928 194 94 1 12 205 7 160 3.20e-24 94.4
MS.gene000342.t1 MTR_3g107717 27.551 196 138 4 15 209 14 206 1.30e-22 91.7
MS.gene000342.t1 MTR_3g107713 31.056 161 108 3 15 175 14 171 1.50e-22 91.7
MS.gene000342.t1 MTR_3g107707 31.056 161 108 3 15 175 14 171 1.59e-22 91.7
MS.gene000342.t1 MTR_8g031810 40.777 103 47 1 13 115 5 93 3.40e-20 82.0
MS.gene000342.t1 MTR_1452s0010 45.570 79 43 0 34 112 1 79 3.54e-20 82.0
MS.gene000342.t1 MTR_1g069245 41.593 113 62 2 9 121 91 199 2.96e-19 82.8
MS.gene000342.t1 MTR_2g090875 30.460 174 107 5 14 175 6 177 1.94e-18 80.1
MS.gene000342.t1 MTR_3g078260 29.282 181 114 5 14 182 8 186 1.23e-17 78.2
MS.gene000342.t1 MTR_4g073250 27.624 181 117 5 14 182 6 184 6.30e-17 76.3
MS.gene000342.t1 MTR_5g022600 29.885 174 108 5 14 175 6 177 8.00e-17 75.9
MS.gene000342.t1 MTR_8g075240 30.682 176 104 6 14 175 6 177 8.42e-17 75.9
MS.gene000342.t1 MTR_2g078000 59.184 49 20 0 75 123 46 94 9.19e-17 73.2
MS.gene000342.t1 MTR_6g087980 29.282 181 114 5 14 182 6 184 1.82e-16 75.1
MS.gene000342.t1 MTR_4g088055 29.070 172 108 5 14 173 6 175 2.47e-16 74.7
MS.gene000342.t1 MTR_1686s0010 34.783 92 60 0 34 125 1 92 2.95e-16 71.6
MS.gene000342.t1 MTR_4g113620 25.301 166 112 4 15 180 14 167 7.76e-16 73.6
MS.gene000342.t1 MTR_1g059590 29.940 167 108 4 17 182 431 589 8.73e-13 67.0
MS.gene000342.t1 MTR_1g059590 29.940 167 108 4 17 182 398 556 8.73e-13 67.0
MS.gene000342.t1 MTR_5g092060 23.214 168 123 2 15 177 105 271 1.34e-12 65.9
MS.gene000342.t1 MTR_3g053480 59.574 47 19 0 10 56 12 58 1.45e-12 61.2
MS.gene000342.t1 MTR_8g099250 64.444 45 16 0 10 54 12 56 2.77e-12 60.5
MS.gene000342.t1 MTR_2g078560 55.556 45 20 0 76 120 54 98 2.89e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000342.t1 AT2G31680 83.410 217 34 2 4 219 2 217 2.53e-131 369
MS.gene000342.t1 AT1G05810 83.796 216 34 1 4 219 2 216 3.55e-131 368
MS.gene000342.t1 AT1G05810 83.796 216 34 1 4 219 48 262 5.05e-131 369
MS.gene000342.t1 AT2G43130 81.019 216 37 1 4 219 2 213 6.56e-127 357
MS.gene000342.t1 AT3G07410 78.571 210 44 1 10 219 8 216 1.00e-117 334
MS.gene000342.t1 AT5G47520 63.801 221 70 4 4 219 5 220 9.48e-96 278
MS.gene000342.t1 AT1G07410 55.769 208 88 2 12 219 10 213 1.57e-83 247
MS.gene000342.t1 AT3G12160 62.500 176 66 0 12 187 13 188 1.08e-82 245
MS.gene000342.t1 AT5G65270 64.740 173 61 0 12 184 15 187 1.20e-82 246
MS.gene000342.t1 AT4G18430 56.938 209 86 2 12 219 11 216 1.29e-82 245
MS.gene000342.t1 AT5G47960 65.318 173 60 0 12 184 13 185 1.86e-82 245
MS.gene000342.t1 AT5G60860 57.692 208 86 1 12 219 11 216 7.04e-82 243
MS.gene000342.t1 AT1G09630 53.365 208 95 1 12 219 10 215 1.23e-81 243
MS.gene000342.t1 AT4G39990 52.607 211 94 2 12 219 15 222 1.88e-81 242
MS.gene000342.t1 AT5G59150 63.158 171 63 0 12 182 10 180 4.92e-81 241
MS.gene000342.t1 AT1G16920 58.173 208 84 2 12 219 11 215 1.09e-80 240
MS.gene000342.t1 AT5G45750 55.769 208 89 2 12 219 11 215 5.50e-80 238
MS.gene000342.t1 AT4G18800 63.429 175 64 0 12 186 11 185 6.41e-80 238
MS.gene000342.t1 AT3G46830 62.573 171 64 0 12 182 10 180 6.77e-80 238
MS.gene000342.t1 AT3G46830 62.573 171 64 0 12 182 10 180 6.77e-80 238
MS.gene000342.t1 AT1G06400 58.470 183 76 0 12 194 11 193 1.76e-78 234
MS.gene000342.t1 AT1G28550 54.808 208 93 1 12 219 11 217 3.17e-78 234
MS.gene000342.t1 AT3G15060 54.808 208 92 1 12 219 11 216 5.35e-78 233
MS.gene000342.t1 AT2G33870 53.846 208 95 1 12 219 11 217 1.45e-77 232
MS.gene000342.t1 AT1G73640 58.140 172 72 0 12 183 11 182 3.59e-71 217
MS.gene000342.t1 AT1G18200 57.803 173 73 0 12 184 11 183 3.33e-70 214
MS.gene000342.t1 AT1G01200 51.429 210 98 3 12 217 26 235 8.67e-69 211
MS.gene000342.t1 AT4G17170 43.541 209 114 2 13 219 5 211 1.29e-58 184
MS.gene000342.t1 AT4G17160 44.175 206 109 2 13 218 5 204 5.17e-57 179
MS.gene000342.t1 AT4G35860 47.126 174 92 0 12 185 4 177 2.85e-56 178
MS.gene000342.t1 AT5G59150 60.630 127 50 0 56 182 3 129 4.09e-56 176
MS.gene000342.t1 AT3G11730 48.780 164 84 0 12 175 6 169 6.69e-51 164
MS.gene000342.t1 AT1G02130 49.682 157 79 0 12 168 6 162 1.91e-50 163
MS.gene000342.t1 AT4G17530 49.045 157 80 0 12 168 6 162 2.48e-50 162
MS.gene000342.t1 AT3G53610 39.904 208 118 4 12 218 13 214 2.23e-49 160
MS.gene000342.t1 AT3G53610 39.904 208 118 4 12 218 13 214 2.23e-49 160
MS.gene000342.t1 AT3G53610 39.904 208 118 4 12 218 13 214 2.23e-49 160
MS.gene000342.t1 AT3G09900 40.191 209 119 3 12 219 13 216 8.77e-49 159
MS.gene000342.t1 AT3G46060 39.423 208 119 4 12 218 13 214 1.33e-48 159
MS.gene000342.t1 AT3G46060 39.423 208 119 4 12 218 13 214 1.33e-48 159
MS.gene000342.t1 AT3G46060 39.423 208 119 4 12 218 13 214 1.33e-48 159
MS.gene000342.t1 AT5G47200 48.408 157 81 0 12 168 6 162 2.15e-48 157
MS.gene000342.t1 AT5G59840 38.942 208 120 4 12 218 13 214 3.28e-48 157
MS.gene000342.t1 AT5G03520 40.191 209 117 4 12 219 13 214 7.07e-48 157
MS.gene000342.t1 AT3G54840 44.767 172 90 2 16 186 35 202 3.25e-46 152
MS.gene000342.t1 AT3G54840 44.444 171 90 2 17 186 2 168 6.46e-46 150
MS.gene000342.t1 AT3G54840 47.097 155 81 1 16 169 35 189 9.34e-46 150
MS.gene000342.t1 AT4G19640 41.250 160 94 0 16 175 12 171 7.89e-45 148
MS.gene000342.t1 AT5G45130 39.216 204 109 4 16 219 12 200 3.26e-44 147
MS.gene000342.t1 AT5G45130 39.109 202 108 4 18 219 22 208 3.57e-43 144
MS.gene000342.t1 AT1G43890 46.199 171 89 3 12 180 11 180 1.76e-42 143
MS.gene000342.t1 AT1G43890 46.199 171 89 3 12 180 11 180 1.76e-42 143
MS.gene000342.t1 AT1G43890 46.199 171 89 3 12 180 11 180 1.76e-42 143
MS.gene000342.t1 AT4G35860 47.656 128 67 0 58 185 4 131 5.47e-42 140
MS.gene000342.t1 AT5G10260 39.490 157 95 0 15 171 10 166 2.02e-41 140
MS.gene000342.t1 AT2G44610 36.275 204 124 3 15 217 10 208 2.28e-40 137
MS.gene000342.t1 AT2G22290 38.854 157 96 0 15 171 10 166 3.45e-40 137
MS.gene000342.t1 AT4G39890 37.975 158 97 1 15 171 10 167 3.74e-39 134
MS.gene000342.t1 AT5G03530 45.062 162 86 3 10 169 9 169 6.02e-38 131
MS.gene000342.t1 AT5G03530 45.062 162 86 3 10 169 9 169 6.02e-38 131
MS.gene000342.t1 AT5G64990 38.961 154 94 0 15 168 8 161 6.34e-38 131
MS.gene000342.t1 AT5G03520 38.372 172 98 4 49 219 40 204 3.55e-37 129
MS.gene000342.t1 AT2G21880 41.975 162 87 3 14 168 9 170 4.36e-37 129
MS.gene000342.t1 AT1G22740 41.317 167 91 3 14 173 8 174 6.73e-37 128
MS.gene000342.t1 AT1G52280 37.791 172 100 3 14 178 8 179 1.69e-35 125
MS.gene000342.t1 AT5G64990 35.673 171 103 1 15 178 8 178 3.27e-35 124
MS.gene000342.t1 AT3G18820 38.095 168 97 3 14 174 8 175 3.51e-35 124
MS.gene000342.t1 AT4G09720 38.953 172 98 3 14 178 8 179 1.50e-34 122
MS.gene000342.t1 AT4G19640 37.580 157 97 1 16 172 12 167 7.97e-34 120
MS.gene000342.t1 AT4G09720 38.418 177 97 4 14 178 8 184 8.01e-34 120
MS.gene000342.t1 AT3G16100 37.209 172 101 3 14 178 8 179 1.64e-33 119
MS.gene000342.t1 AT4G19640 39.231 130 79 0 16 145 12 141 2.32e-33 117
MS.gene000342.t1 AT5G46025 55.660 106 46 1 76 180 6 111 5.38e-33 115
MS.gene000342.t1 AT4G09720 36.612 183 98 3 14 178 8 190 1.20e-32 117
MS.gene000342.t1 AT3G09910 39.535 172 101 3 10 179 9 179 4.01e-32 115
MS.gene000342.t1 AT3G09910 39.535 172 101 3 10 179 9 179 4.01e-32 115
MS.gene000342.t1 AT1G49300 37.654 162 94 3 14 168 8 169 5.40e-32 115
MS.gene000342.t1 AT1G49300 37.654 162 94 3 14 168 8 169 5.40e-32 115
MS.gene000342.t1 AT3G09910 35.648 216 113 5 10 219 9 204 9.18e-32 115
MS.gene000342.t1 AT3G09910 35.648 216 113 5 10 219 9 204 9.18e-32 115
MS.gene000342.t1 AT3G09910 35.648 216 113 5 10 219 9 204 9.18e-32 115
MS.gene000342.t1 AT5G39620 34.574 188 115 4 11 191 2 188 3.19e-30 111
MS.gene000342.t1 AT2G21880 40.000 145 80 3 31 168 1 145 1.30e-28 106
MS.gene000342.t1 AT2G21880 40.000 145 80 3 31 168 1 145 1.30e-28 106
MS.gene000342.t1 AT2G21880 37.037 162 87 4 14 168 9 162 3.34e-27 103
MS.gene000342.t1 AT4G09720 37.931 145 83 3 41 178 1 145 2.03e-23 92.8
MS.gene000342.t1 AT4G09720 37.931 145 83 3 41 178 1 145 2.03e-23 92.8
MS.gene000342.t1 AT4G09720 37.931 145 83 3 41 178 1 145 2.03e-23 92.8
MS.gene000342.t1 AT5G55080 29.814 161 110 3 15 175 14 171 2.39e-23 94.0
MS.gene000342.t1 AT5G20020 31.056 161 108 3 15 175 14 171 1.19e-22 92.0
MS.gene000342.t1 AT5G20010 31.056 161 108 3 15 175 14 171 1.83e-22 91.7
MS.gene000342.t1 AT5G55190 31.056 161 108 3 15 175 14 171 2.06e-22 91.3
MS.gene000342.t1 AT2G44690 33.333 180 106 6 14 181 18 195 3.00e-22 90.5
MS.gene000342.t1 AT4G35020 29.885 174 108 5 14 175 6 177 1.77e-17 77.8
MS.gene000342.t1 AT4G35020 29.885 174 108 5 14 175 6 177 1.77e-17 77.8
MS.gene000342.t1 AT4G35020 29.885 174 108 5 14 175 6 177 1.77e-17 77.8
MS.gene000342.t1 AT5G62880 29.310 174 109 5 14 175 8 179 1.80e-17 78.2
MS.gene000342.t1 AT5G45970 30.233 172 106 6 14 173 6 175 2.95e-17 77.0
MS.gene000342.t1 AT1G75840 30.682 176 104 6 14 175 6 177 9.90e-17 75.5
MS.gene000342.t1 AT3G48040 28.736 174 110 5 14 175 8 179 1.07e-16 75.9
MS.gene000342.t1 AT4G28950 28.492 179 116 5 14 182 6 182 1.41e-16 75.5
MS.gene000342.t1 AT4G35950 30.682 176 104 5 14 175 6 177 1.53e-16 75.1
MS.gene000342.t1 AT3G51300 30.233 172 106 5 16 175 8 177 1.68e-16 75.1
MS.gene000342.t1 AT2G17800 31.250 176 103 6 14 175 6 177 2.24e-16 74.7
MS.gene000342.t1 AT2G17800 31.250 176 103 6 14 175 6 177 2.24e-16 74.7
MS.gene000342.t1 AT1G20090 28.161 174 111 5 14 175 5 176 3.50e-16 74.3
MS.gene000342.t1 AT5G62880 31.655 139 85 4 14 151 8 137 3.42e-14 68.6
MS.gene000342.t1 AT4G08190 46.875 64 31 1 105 168 67 127 6.96e-14 66.2
MS.gene000342.t1 AT4G35020 33.333 114 73 3 16 128 8 119 1.61e-13 65.9
MS.gene000342.t1 AT4G35020 33.333 114 73 3 16 128 8 119 1.61e-13 65.9
MS.gene000342.t1 AT3G48040 31.034 145 90 4 14 157 8 143 1.71e-13 66.6

Find 36 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTAAAGCTCAGATT+TGG 0.265031 7.1:-88026502 MS.gene000342:CDS
TTCAACTCCGATCGTTGCTT+TGG 0.339177 7.1:+88026556 None:intergenic
TTTCATGGAAACCTCTGCTT+TGG 0.343525 7.1:-88025098 MS.gene000342:CDS
AAAATAGCCTTTGCTTTGCT+TGG 0.356179 7.1:+88024931 None:intergenic
AGGCTGAATTATCTGTCAAC+AGG 0.387029 7.1:-88024985 MS.gene000342:CDS
CTCTTCAAAGTCGTTATAAT+CGG 0.399662 7.1:-88026644 MS.gene000342:CDS
AGAAGTTAAAGCTCAGATTT+GGG 0.412522 7.1:-88026501 MS.gene000342:CDS
GGTGGGAAACAAGTGTGATT+TGG 0.440037 7.1:-88025182 MS.gene000342:CDS
GCTTATTATCGTGGTGCTGT+TGG 0.462704 7.1:-88026440 MS.gene000342:CDS
AGCGTATCGTGAGAGGAGAT+TGG 0.495642 7.1:+88026601 None:intergenic
AGATATACAACAATGTTAGC+AGG 0.522828 7.1:-88025033 MS.gene000342:CDS
GTTACTTCTGCTTATTATCG+TGG 0.541558 7.1:-88026449 MS.gene000342:CDS
CCTCAACTCAGACACTTACA+AGG 0.545261 7.1:-88025005 MS.gene000342:CDS
AGTTCAGAAGACGAAGGAGG+AGG 0.552796 7.1:-88026677 MS.gene000342:CDS
AAAAGTCTTGCGGAATCAGA+AGG 0.554276 7.1:-88025127 MS.gene000342:CDS
GCTCAGATTTGGGATACTGC+TGG 0.571910 7.1:-88026491 MS.gene000342:CDS
ATAATCGGTGACTCCGCCGT+TGG 0.575874 7.1:-88026629 MS.gene000342:CDS
TACGACGGTGGCAATGATGC+TGG 0.577423 7.1:-88025203 MS.gene000342:CDS
AGAGATGTGAGCATTGAAGA+AGG 0.582505 7.1:-88025151 MS.gene000342:CDS
CCTTGTAAGTGTCTGAGTTG+AGG 0.585872 7.1:+88025005 None:intergenic
CGTTGCGAGCGTATCGTGAG+AGG 0.597765 7.1:+88026594 None:intergenic
ATTTGTAGAGTCCAAAGCAG+AGG 0.603895 7.1:+88025087 None:intergenic
CTTCATTCCAAAGCAACGAT+CGG 0.608692 7.1:-88026563 MS.gene000342:CDS
GAGGAGATTGGATTTACCAA+CGG 0.633949 7.1:+88026613 None:intergenic
TCTACATCCAAGCAAAGCAA+AGG 0.634847 7.1:-88024938 MS.gene000342:CDS
TGAAGAAGGCAAAAGTCTTG+CGG 0.635809 7.1:-88025137 MS.gene000342:CDS
GGCTGAATTATCTGTCAACA+GGG 0.647451 7.1:-88024984 MS.gene000342:CDS
TCAAGTTCAGAAGACGAAGG+AGG 0.656404 7.1:-88026680 MS.gene000342:CDS
AGGGTCAGTCTTGTTAACAA+TGG 0.671243 7.1:-88024965 MS.gene000342:CDS
ATACAACAATGTTAGCAGGA+AGG 0.674114 7.1:-88025029 MS.gene000342:CDS
GACGGTGGCAATGATGCTGG+TGG 0.677201 7.1:-88025200 MS.gene000342:CDS
TGCAGCTCATTGTGATACGA+CGG 0.692981 7.1:-88025218 MS.gene000342:intron
AGCTCATTGTGATACGACGG+TGG 0.708088 7.1:-88025215 MS.gene000342:CDS
GAGATTGGATTTACCAACGG+CGG 0.710073 7.1:+88026616 None:intergenic
ACGGTGGCAATGATGCTGGT+GGG 0.711154 7.1:-88025199 MS.gene000342:CDS
ATGTCAAGTTCAGAAGACGA+AGG 0.732589 7.1:-88026683 MS.gene000342:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATATATATTTGATTACA+GGG - chr7.1:88025488-88025507 MS.gene000342:intron 15.0%
!! TATGATATATATTTGATTAC+AGG - chr7.1:88025487-88025506 MS.gene000342:intron 15.0%
!!! GTTAATTTATGTTATGTTTT+TGG - chr7.1:88025296-88025315 MS.gene000342:intron 15.0%
!! AAAACTATGTATAATTGCTA+TGG - chr7.1:88026253-88026272 MS.gene000342:intron 20.0%
!! AACACTTACTATTAGAAATA+TGG + chr7.1:88026107-88026126 None:intergenic 20.0%
!! AGTCTATATATACTTTCTAT+AGG + chr7.1:88025626-88025645 None:intergenic 20.0%
!! TACTAGTATATATCAATGAT+AGG - chr7.1:88025740-88025759 MS.gene000342:intron 20.0%
!!! ATTATACATAGTTTTTAGAG+AGG + chr7.1:88026248-88026267 None:intergenic 20.0%
!!! TTATGTTATGTTTTTGGTTT+TGG - chr7.1:88025302-88025321 MS.gene000342:intron 20.0%
! AAGGGTGGAATTAAATTAAT+TGG - chr7.1:88025691-88025710 MS.gene000342:intron 25.0%
! AAGTATATATAGACTCTGTT+TGG - chr7.1:88025631-88025650 MS.gene000342:intron 25.0%
! AATGTTAAGCACAAATATGT+AGG + chr7.1:88025998-88026017 None:intergenic 25.0%
! ATGTTAAGCACAAATATGTA+GGG + chr7.1:88025997-88026016 None:intergenic 25.0%
! CTTACTATTAGAAATATGGA+AGG + chr7.1:88026103-88026122 None:intergenic 25.0%
! TAATTAATCTCACATTCCAA+AGG - chr7.1:88025411-88025430 MS.gene000342:intron 25.0%
!!! AGTGCATTTTGAAAACTAAA+AGG + chr7.1:88025959-88025978 None:intergenic 25.0%
!!! CAATTTCTCCTAGTTTTTAT+AGG + chr7.1:88025893-88025912 None:intergenic 25.0%
!!! GTGCATTTTGAAAACTAAAA+GGG + chr7.1:88025958-88025977 None:intergenic 25.0%
!!! TGCATTTTGAAAACTAAAAG+GGG + chr7.1:88025957-88025976 None:intergenic 25.0%
!!! TTTTTTTTTAGTGAGGATAC+AGG - chr7.1:88026148-88026167 MS.gene000342:intron 25.0%
AAAAACACTAGAGTACATTC+AGG - chr7.1:88026052-88026071 MS.gene000342:intron 30.0%
AAGAAGTTAAAGCTCAGATT+TGG - chr7.1:88025097-88025116 MS.gene000342:CDS 30.0%
AGAAGTTAAAGCTCAGATTT+GGG - chr7.1:88025098-88025117 MS.gene000342:CDS 30.0%
AGATATACAACAATGTTAGC+AGG - chr7.1:88026566-88026585 MS.gene000342:CDS 30.0%
CTATGGTTAATTGTGATTGT+GGG - chr7.1:88026270-88026289 MS.gene000342:intron 30.0%
CTCTTCAAAGTCGTTATAAT+CGG - chr7.1:88024955-88024974 MS.gene000342:CDS 30.0%
TAGGAAGTTACTTGATTACA+TGG - chr7.1:88025759-88025778 MS.gene000342:intron 30.0%
TGTGAAGACCTATAAAAACT+AGG - chr7.1:88025882-88025901 MS.gene000342:intron 30.0%
TTGTGAAAAAATGTTCGATG+TGG - chr7.1:88026125-88026144 MS.gene000342:intron 30.0%
!! ATCAGAAGGTTTGTTTTTCA+TGG - chr7.1:88026486-88026505 MS.gene000342:CDS 30.0%
!!! GAATGTACTCTAGTGTTTTT+TGG + chr7.1:88026052-88026071 None:intergenic 30.0%
!!! GATGTGGTTTTTTTTTAGTG+AGG - chr7.1:88026141-88026160 MS.gene000342:intron 30.0%
AAAATAGCCTTTGCTTTGCT+TGG + chr7.1:88026671-88026690 None:intergenic 35.0%
AAACTTTGATATTGCGGTTG+CGG - chr7.1:88025804-88025823 MS.gene000342:intron 35.0%
AATCTCACTAGTACACTCTA+AGG - chr7.1:88025672-88025691 MS.gene000342:intron 35.0%
AATGCCTAAGTATAGCTAGA+AGG - chr7.1:88026324-88026343 MS.gene000342:intron 35.0%
ATACAACAATGTTAGCAGGA+AGG - chr7.1:88026570-88026589 MS.gene000342:CDS 35.0%
ATCTCACTAGTACACTCTAA+GGG - chr7.1:88025673-88025692 MS.gene000342:intron 35.0%
CGTTTAAAGAGATCTCATGT+AGG - chr7.1:88026210-88026229 MS.gene000342:intron 35.0%
GCTATGGTTAATTGTGATTG+TGG - chr7.1:88026269-88026288 MS.gene000342:intron 35.0%
GTTACTTCTGCTTATTATCG+TGG - chr7.1:88025150-88025169 MS.gene000342:CDS 35.0%
TAATCTCACATTCCAAAGGA+AGG - chr7.1:88025415-88025434 MS.gene000342:intron 35.0%
TTTGCCAATGTGGTTGTAAT+TGG - chr7.1:88025845-88025864 MS.gene000342:intron 35.0%
! GAGCAAATATTTTGCCAATG+TGG - chr7.1:88025835-88025854 MS.gene000342:intron 35.0%
! GATTACAGGGTTTGAAATTG+AGG - chr7.1:88025501-88025520 MS.gene000342:intron 35.0%
! TCAACCTTCTAGCTATACTT+AGG + chr7.1:88026331-88026350 None:intergenic 35.0%
!!! AGAACTACTTTTGATAGCGT+TGG - chr7.1:88025210-88025229 MS.gene000342:CDS 35.0%
!!! TTTTTTAGTGAGGATACAGG+AGG - chr7.1:88026151-88026170 MS.gene000342:intron 35.0%
AAAAGTCTTGCGGAATCAGA+AGG - chr7.1:88026472-88026491 MS.gene000342:CDS 40.0%
AGCACCAATTACAACCACAT+TGG + chr7.1:88025852-88025871 None:intergenic 40.0%
AGGCTGAATTATCTGTCAAC+AGG - chr7.1:88026614-88026633 MS.gene000342:CDS 40.0%
AGGTCTAGTTATGTTCCTAG+CGG + chr7.1:88026083-88026102 None:intergenic 40.0%
ATCCATCTCTTTCCTTCCTT+TGG + chr7.1:88025430-88025449 None:intergenic 40.0%
ATGAGCAATTATGGCAGATG+CGG - chr7.1:88025568-88025587 MS.gene000342:intron 40.0%
ATGTCAAGTTCAGAAGACGA+AGG - chr7.1:88024916-88024935 MS.gene000342:CDS 40.0%
ATTTGTAGAGTCCAAAGCAG+AGG + chr7.1:88026515-88026534 None:intergenic 40.0%
CGCTGCAAACTTTGATATTG+CGG - chr7.1:88025798-88025817 MS.gene000342:intron 40.0%
CGGCAAAGTATGAGCAATTA+TGG - chr7.1:88025559-88025578 MS.gene000342:intron 40.0%
CTTCATTCCAAAGCAACGAT+CGG - chr7.1:88025036-88025055 MS.gene000342:CDS 40.0%
GAGGAGATTGGATTTACCAA+CGG + chr7.1:88024989-88025008 None:intergenic 40.0%
GGCTGAATTATCTGTCAACA+GGG - chr7.1:88026615-88026634 MS.gene000342:CDS 40.0%
TCTACATCCAAGCAAAGCAA+AGG - chr7.1:88026661-88026680 MS.gene000342:CDS 40.0%
TGAAGAAGGCAAAAGTCTTG+CGG - chr7.1:88026462-88026481 MS.gene000342:CDS 40.0%
TGCCGCAAACTTTGATATTG+CGG - chr7.1:88025539-88025558 MS.gene000342:intron 40.0%
TGCCGCAATATCAAAGTTTG+CGG + chr7.1:88025544-88025563 None:intergenic 40.0%
TTCCAAAGGAAGGAAAGAGA+TGG - chr7.1:88025425-88025444 MS.gene000342:intron 40.0%
TTTCATGGAAACCTCTGCTT+TGG - chr7.1:88026501-88026520 MS.gene000342:CDS 40.0%
! CACAAATATGTAGGGAGTAC+AGG + chr7.1:88025989-88026008 None:intergenic 40.0%
!! AGAGATGTGAGCATTGAAGA+AGG - chr7.1:88026448-88026467 MS.gene000342:CDS 40.0%
!! AGGGTCAGTCTTGTTAACAA+TGG - chr7.1:88026634-88026653 MS.gene000342:CDS 40.0%
!! TGTGAAGACAGCTTTTGAGA+TGG - chr7.1:88026534-88026553 MS.gene000342:CDS 40.0%
CCTCAACTCAGACACTTACA+AGG - chr7.1:88026594-88026613 MS.gene000342:CDS 45.0%
CCTTGTAAGTGTCTGAGTTG+AGG + chr7.1:88026597-88026616 None:intergenic 45.0%
GAGATTGGATTTACCAACGG+CGG + chr7.1:88024986-88025005 None:intergenic 45.0%
GGTGGGAAACAAGTGTGATT+TGG - chr7.1:88026417-88026436 MS.gene000342:CDS 45.0%
GTGTCAGATAGAGACGTAAC+AGG + chr7.1:88026026-88026045 None:intergenic 45.0%
TCAAGTTCAGAAGACGAAGG+AGG - chr7.1:88024919-88024938 MS.gene000342:CDS 45.0%
TGCAGCTCATTGTGATACGA+CGG - chr7.1:88026381-88026400 MS.gene000342:CDS 45.0%
TTCAACTCCGATCGTTGCTT+TGG + chr7.1:88025046-88025065 None:intergenic 45.0%
! AATTGCATTGACCTCCTCTG+AGG - chr7.1:88025908-88025927 MS.gene000342:intron 45.0%
! AGGGTTTGAAATTGAGGTCG+TGG - chr7.1:88025507-88025526 MS.gene000342:intron 45.0%
! TCACTAGTACACTCTAAGGG+TGG - chr7.1:88025676-88025695 MS.gene000342:intron 45.0%
!! GCTTATTATCGTGGTGCTGT+TGG - chr7.1:88025159-88025178 MS.gene000342:CDS 45.0%
!!! CTTTTGATAGCGTTGGTCGT+TGG - chr7.1:88025217-88025236 MS.gene000342:intron 45.0%
AGCGTATCGTGAGAGGAGAT+TGG + chr7.1:88025001-88025020 None:intergenic 50.0%
AGCTCATTGTGATACGACGG+TGG - chr7.1:88026384-88026403 MS.gene000342:CDS 50.0%
AGTTCAGAAGACGAAGGAGG+AGG - chr7.1:88024922-88024941 MS.gene000342:CDS 50.0%
CACTTAGTGAGACACCTCAG+AGG + chr7.1:88025925-88025944 None:intergenic 50.0%
TTAGTGAGACACCTCAGAGG+AGG + chr7.1:88025922-88025941 None:intergenic 50.0%
! ATATGTAGGGAGTACAGGCG+AGG + chr7.1:88025984-88026003 None:intergenic 50.0%
! GCTCAGATTTGGGATACTGC+TGG - chr7.1:88025108-88025127 MS.gene000342:CDS 50.0%
!! TACATTCAGGTTGTGCCGCT+AGG - chr7.1:88026065-88026084 MS.gene000342:intron 50.0%
ACGGTGGCAATGATGCTGGT+GGG - chr7.1:88026400-88026419 MS.gene000342:CDS 55.0%
ATAATCGGTGACTCCGCCGT+TGG - chr7.1:88024970-88024989 MS.gene000342:CDS 55.0%
TACGACGGTGGCAATGATGC+TGG - chr7.1:88026396-88026415 MS.gene000342:CDS 55.0%
CGTTGCGAGCGTATCGTGAG+AGG + chr7.1:88025008-88025027 None:intergenic 60.0%
GACGGTGGCAATGATGCTGG+TGG - chr7.1:88026399-88026418 MS.gene000342:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 88024916 88026705 88024916 ID=MS.gene000342
chr7.1 mRNA 88024916 88026705 88024916 ID=MS.gene000342.t1;Parent=MS.gene000342
chr7.1 exon 88026366 88026705 88026366 ID=MS.gene000342.t1.exon1;Parent=MS.gene000342.t1
chr7.1 CDS 88026366 88026705 88026366 ID=cds.MS.gene000342.t1;Parent=MS.gene000342.t1
chr7.1 exon 88024916 88025235 88024916 ID=MS.gene000342.t1.exon2;Parent=MS.gene000342.t1
chr7.1 CDS 88024916 88025235 88024916 ID=cds.MS.gene000342.t1;Parent=MS.gene000342.t1
Gene Sequence

>MS.gene000342

ATGTCAAGTTCAGAAGACGAAGGAGGAGGAGAAGAATATCTCTTCAAAGTCGTTATAATCGGTGACTCCGCCGTTGGTAAATCCAATCTCCTCTCACGATACGCTCGCAACGAGTTCAATCTTCATTCCAAAGCAACGATCGGAGTTGAATTTCAAACTCAGAGCTTAGAAATTGATTCTAAAGAAGTTAAAGCTCAGATTTGGGATACTGCTGGTCAAGAACGTTTTCGTGCTGTTACTTCTGCTTATTATCGTGGTGCTGTTGGTGCTCTTATTGTTTATGATATTTCTCGCAGAACTACTTTTGATAGCGTTGGTCGTTGGCTCGATGAACTCAAGAGTTAGTAGTGCTTTCTTCTTTTTCTAGATCTGATCTTTTAGTTAATTTATGTTATGTTTTTGGTTTTGGATCTCGTAGTTTATGTTAATCTTGATTTTGCTATGTGAAATTGACTGTGTTGATTATATTATATATAATCTTGATTGTTCGTAGCTTAATTAATCTCACATTCCAAAGGAAGGAAAGAGATGGATAATAGAAAAGTGAACTATGTATCTGTGAGCAAAACATTATGATATATATTTGATTACAGGGTTTGAAATTGAGGTCGTGGTTACAGTTTTGCCGCAAACTTTGATATTGCGGCAAAGTATGAGCAATTATGGCAGATGCGGTCGTAATTGCTGCTGTCGTTATGTCGTGATGACCTATAGAAAGTATATATAGACTCTGTTTGGATAATCTGCTTGCAGTTTAATCTCACTAGTACACTCTAAGGGTGGAATTAAATTAATTGGAAAGAAATAGATAATAAAACTTGAACTACTAGTATATATCAATGATAGGAAGTTACTTGATTACATGGTTCGAAATTGCAGTCGCGCTGCAAACTTTGATATTGCGGTTGCGGCAAAATATGAGCAAATATTTTGCCAATGTGGTTGTAATTGGTGCTGTCATGATGTTGTGAAGACCTATAAAAACTAGGAGAAATTGCATTGACCTCCTCTGAGGTGTCTCACTAAGTGACACTCACACCCCTTTTAGTTTTCAAAATGCACTCACCTCGCCTGTACTCCCTACATATTTGTGCTTAACATTTTTTTCCTGTTACGTCTCTATCTGACACTCACCAAAAAACACTAGAGTACATTCAGGTTGTGCCGCTAGGAACATAACTAGACCTTCCATATTTCTAATAGTAAGTGTTGTGAAAAAATGTTCGATGTGGTTTTTTTTTAGTGAGGATACAGGAGGTGAGTGCTTTTTGAATATCAGAGAGCTTGTCAATGTCGTTTAAAGAGATCTCATGTAGGTCAATGCAGTTTCCTCTCTAAAAACTATGTATAATTGCTATGGTTAATTGTGATTGTGGGCTGTAATTTAAAACTGTTTGATTATATAATTAATGCCTAAGTATAGCTAGAAGGTTGAAGTTTTTGTTTGTTTGATAACTGTGATGCATGCAGCTCATTGTGATACGACGGTGGCAATGATGCTGGTGGGAAACAAGTGTGATTTGGATAATATAAGAGATGTGAGCATTGAAGAAGGCAAAAGTCTTGCGGAATCAGAAGGTTTGTTTTTCATGGAAACCTCTGCTTTGGACTCTACAAATGTGAAGACAGCTTTTGAGATGGTTATTAGAGAGATATACAACAATGTTAGCAGGAAGGTCCTCAACTCAGACACTTACAAGGCTGAATTATCTGTCAACAGGGTCAGTCTTGTTAACAATGGCAACTCTACATCCAAGCAAAGCAAAGGCTATTTTTCTTGCTGTTCCTGA
Protein sequence

>MS.gene000342.t1

MSSSEDEGGGEEYLFKVVIIGDSAVGKSNLLSRYARNEFNLHSKATIGVEFQTQSLEIDSKEVKAQIWDTAGQERFRAVTSAYYRGAVGALIVYDISRRTTFDSVGRWLDELKTHCDTTVAMMLVGNKCDLDNIRDVSIEEGKSLAESEGLFFMETSALDSTNVKTAFEMVIREIYNNVSRKVLNSDTYKAELSVNRVSLVNNGNSTSKQSKGYFSCCS