AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30946


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30946.t1 MTR_2g096610 97.297 148 4 0 1 148 1 148 1.57e-100 285
MS.gene30946.t1 MTR_4g063280 90.909 132 11 1 18 148 14 145 1.36e-79 232
MS.gene30946.t1 MTR_4g071150 86.842 152 16 2 1 148 1 152 1.14e-78 229
MS.gene30946.t1 MTR_4g064005 87.333 150 15 2 1 148 1 148 4.55e-78 228
MS.gene30946.t1 MTR_2g096570 87.023 131 13 1 22 148 23 153 1.28e-76 224
MS.gene30946.t1 MTR_4g064967 91.339 127 9 1 22 148 24 148 1.50e-76 224
MS.gene30946.t1 MTR_4g063410 84.000 150 20 2 1 148 1 148 6.11e-75 220
MS.gene30946.t1 MTR_7g113160 81.061 132 25 0 9 140 13 144 2.27e-74 218
MS.gene30946.t1 MTR_8g086640 89.394 132 12 1 1 130 1 132 7.83e-72 212
MS.gene30946.t1 MTR_7g093910 73.810 126 31 1 2 127 4 127 1.05e-60 184
MS.gene30946.t1 MTR_7g108320 74.400 125 29 1 3 127 6 127 3.64e-60 182
MS.gene30946.t1 MTR_7g114060 73.810 126 29 2 3 127 2 124 1.22e-59 181
MS.gene30946.t1 MTR_7g114040 73.810 126 29 2 3 127 2 124 1.22e-59 181
MS.gene30946.t1 MTR_2g082510 78.378 111 24 0 17 127 11 121 5.13e-59 179
MS.gene30946.t1 MTR_2g082220 79.630 108 22 0 20 127 14 121 2.02e-58 177
MS.gene30946.t1 MTR_6g005150 65.354 127 30 3 22 147 26 139 1.71e-50 158
MS.gene30946.t1 MTR_7g114050 61.029 136 42 2 5 140 4 128 4.62e-47 149
MS.gene30946.t1 MTR_4g063300 74.286 105 22 1 43 147 10 109 1.88e-45 144
MS.gene30946.t1 MTR_1g011750 60.177 113 43 2 19 130 23 134 6.88e-37 123
MS.gene30946.t1 MTR_1g011850 60.177 113 43 2 19 130 23 134 6.88e-37 123
MS.gene30946.t1 MTR_1g114050 57.983 119 48 2 9 126 10 127 9.52e-37 123
MS.gene30946.t1 MTR_1g071040 62.500 104 37 2 24 126 28 130 2.59e-35 119
MS.gene30946.t1 MTR_1g069770 48.958 96 48 1 43 137 9 104 4.63e-25 92.4
MS.gene30946.t1 MTR_4g048490 43.810 105 30 2 22 126 26 101 8.60e-17 71.2
MS.gene30946.t1 MTR_7g010440 70.588 51 15 0 76 126 122 172 1.52e-16 74.7
MS.gene30946.t1 MTR_4g005910 74.419 43 11 0 85 127 4 46 2.31e-16 68.9
MS.gene30946.t1 MTR_7g027610 41.818 110 38 3 18 126 10 94 1.02e-15 68.9
MS.gene30946.t1 MTR_2g096590 75.000 40 10 0 108 147 12 51 6.54e-14 62.4
MS.gene30946.t1 MTR_0653s0030 54.545 55 25 0 53 107 43 97 3.49e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30946.t1 AT5G02560 82.353 153 22 2 1 148 1 153 9.32e-80 233
MS.gene30946.t1 AT5G27670 77.551 147 32 1 2 148 5 150 5.26e-76 223
MS.gene30946.t1 AT5G02560 71.186 177 22 3 1 148 1 177 9.44e-75 221
MS.gene30946.t1 AT5G59870 72.667 150 39 1 1 148 1 150 5.48e-73 215
MS.gene30946.t1 AT1G51060 80.180 111 22 0 17 127 11 121 2.29e-60 182
MS.gene30946.t1 AT1G08880 72.800 125 30 1 7 127 3 127 9.11e-59 179
MS.gene30946.t1 AT4G27230 77.477 111 25 0 17 127 11 121 1.28e-58 178
MS.gene30946.t1 AT4G27230 77.477 111 25 0 17 127 11 121 1.28e-58 178
MS.gene30946.t1 AT5G54640 78.378 111 24 0 17 127 11 121 1.65e-58 178
MS.gene30946.t1 AT1G54690 73.770 122 28 1 10 127 6 127 2.29e-58 178
MS.gene30946.t1 AT3G20670 77.477 111 25 0 17 127 11 121 2.84e-58 177
MS.gene30946.t1 AT2G38810 59.292 113 44 2 15 126 21 132 7.64e-37 123
MS.gene30946.t1 AT2G38810 59.292 113 44 2 15 126 21 132 7.64e-37 123
MS.gene30946.t1 AT2G38810 59.292 113 44 2 15 126 21 132 7.64e-37 123
MS.gene30946.t1 AT2G38810 59.292 113 44 2 15 126 21 132 7.64e-37 123
MS.gene30946.t1 AT1G52740 62.857 105 37 2 23 126 27 130 1.35e-35 120
MS.gene30946.t1 AT3G54560 58.716 109 43 2 23 130 29 136 1.92e-34 117
MS.gene30946.t1 AT3G54560 58.716 109 43 2 23 130 29 136 1.92e-34 117
MS.gene30946.t1 AT4G13570 48.913 92 45 2 30 120 28 118 1.65e-20 81.3
MS.gene30946.t1 AT4G13570 48.352 91 45 2 31 120 35 124 4.62e-20 80.5

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTCACTGCCTTCTTCCT+TGG 0.238367 2.2:+5476543 None:intergenic
CTAAGCCTTCTTTGGAGATT+TGG 0.253652 2.2:+5476078 None:intergenic
GAAGCCTTCTCATTCTTCTT+AGG 0.258486 2.2:+5476130 None:intergenic
AACTAAATCTAAGCCTTCTT+TGG 0.259152 2.2:+5476070 None:intergenic
TTCTTCTTAGGTAAAAGAAT+TGG 0.279364 2.2:+5476142 None:intergenic
GGTAGCCTTGGATGGAGATT+TGG 0.359896 2.2:+5476099 None:intergenic
AAAAGAATTGGGTTGATGTT+TGG 0.366899 2.2:+5476154 None:intergenic
ACCAGCTCCCTTCTTCGTCT+TGG 0.372930 2.2:+5476581 None:intergenic
CTTTGGAGATTTGGTAGCCT+TGG 0.379306 2.2:+5476087 None:intergenic
AGAACAGCAGCTAGGTAAAC+AGG 0.386139 2.2:+5476429 None:intergenic
AAGGGAGCTGGTGGAAGGAA+AGG 0.395526 2.2:-5476570 MS.gene30946:CDS
CAATTTCCAGTTGGAAGAAT+CGG 0.397925 2.2:-5476501 MS.gene30946:CDS
TCTTCTTAGGTAAAAGAATT+GGG 0.411045 2.2:+5476143 None:intergenic
TTGTGTTCAGGTTCTTGAAT+TGG 0.420978 2.2:-5476305 MS.gene30946:intron
TACGCTCAGCGTGTTGGTAC+TGG 0.452334 2.2:-5476456 MS.gene30946:CDS
CACGTGACAACAAGAAGAAC+AGG 0.464610 2.2:-5476270 MS.gene30946:CDS
TGGAAATTGAAGACCAGCTC+TGG 0.468882 2.2:+5476515 None:intergenic
TAACGACCGATTCTTCCAAC+TGG 0.472426 2.2:+5476495 None:intergenic
GTTCAGGTTCTTGAATTGGC+TGG 0.491391 2.2:-5476301 MS.gene30946:intron
CATCCCTAGGCATTTGCTGT+TGG 0.495579 2.2:-5476245 MS.gene30946:CDS
GAAAGGAGGTCCAAGGAAGA+AGG 0.497415 2.2:-5476553 MS.gene30946:CDS
CGCAAGCACCAAGACGAAGA+AGG 0.504301 2.2:-5476589 MS.gene30946:CDS
GAACTTGGAAAGTTGCTAGC+TGG 0.514674 2.2:-5476205 MS.gene30946:CDS
ATCGGTCGTTACTTGAAGAA+AGG 0.516352 2.2:-5476483 MS.gene30946:CDS
GGCTACCAAATCTCCAAAGA+AGG 0.519492 2.2:-5476083 MS.gene30946:CDS
GGAGATTTGGTAGCCTTGGA+TGG 0.524077 2.2:+5476091 None:intergenic
GTGAGGAATGATGAAGAACT+TGG 0.529342 2.2:-5476220 MS.gene30946:CDS
ACCAAGACGAAGAAGGGAGC+TGG 0.554503 2.2:-5476582 MS.gene30946:CDS
TTTACCTAAGAAGAATGAGA+AGG 0.555333 2.2:-5476134 MS.gene30946:CDS
CGAAGAAGGGAGCTGGTGGA+AGG 0.557443 2.2:-5476575 MS.gene30946:CDS
GCAAGCACCAAGACGAAGAA+GGG 0.557656 2.2:-5476588 MS.gene30946:CDS
TCTTGAATATCTTGCTGCTG+AGG 0.561765 2.2:-5476409 MS.gene30946:intron
AAATTGAAGACCAGCTCTGG+TGG 0.562253 2.2:+5476518 None:intergenic
GTGACAAGATCCACCAGAGC+TGG 0.565492 2.2:-5476528 MS.gene30946:CDS
ACTGCCAACAGCAAATGCCT+AGG 0.578012 2.2:+5476241 None:intergenic
GGAAGATACGCTCAGCGTGT+TGG 0.585684 2.2:-5476462 MS.gene30946:CDS
TGCTACCAAATCTCCATCCA+AGG 0.591170 2.2:-5476104 MS.gene30946:CDS
GCTGGTCTTCAATTTCCAGT+TGG 0.598252 2.2:-5476510 MS.gene30946:CDS
GATATTCAAGAACAGCAGCT+AGG 0.600688 2.2:+5476421 None:intergenic
GGAGCTGGTGGAAGGAAAGG+AGG 0.602867 2.2:-5476567 MS.gene30946:CDS
GGCATTTGCTGTTGGCAGTG+AGG 0.603106 2.2:-5476237 MS.gene30946:CDS
CTGCCAACAGCAAATGCCTA+GGG 0.606589 2.2:+5476242 None:intergenic
AAGACGAAGAAGGGAGCTGG+TGG 0.607040 2.2:-5476579 MS.gene30946:CDS
AGAAGAACAGGATCATCCCT+AGG 0.619121 2.2:-5476258 MS.gene30946:CDS
GTGGAAGGAAAGGAGGTCCA+AGG 0.640933 2.2:-5476560 MS.gene30946:CDS
GCTGGAGTTACAATTGCACA+TGG 0.698871 2.2:-5476187 MS.gene30946:CDS
GGAGTTACAATTGCACATGG+TGG 0.781867 2.2:-5476184 MS.gene30946:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAATTTCATCAAAATT+AGG + chr2.2:5476342-5476361 None:intergenic 15.0%
!! TTTCAATTTCATCAAAATTA+GGG + chr2.2:5476341-5476360 None:intergenic 15.0%
!! CAAATTTGAAAATCAAAAGA+GGG + chr2.2:5476309-5476328 None:intergenic 20.0%
!! TCAAATTTGAAAATCAAAAG+AGG + chr2.2:5476310-5476329 None:intergenic 20.0%
! TCTTCTTAGGTAAAAGAATT+GGG + chr2.2:5476532-5476551 None:intergenic 25.0%
! TTCTTCTTAGGTAAAAGAAT+TGG + chr2.2:5476533-5476552 None:intergenic 25.0%
!!! TTGAAATTTGATTTGTGTTC+AGG - chr2.2:5476355-5476374 MS.gene30946:intron 25.0%
TTTACCTAAGAAGAATGAGA+AGG - chr2.2:5476538-5476557 MS.gene30946:CDS 30.0%
!! AAAAGAATTGGGTTGATGTT+TGG + chr2.2:5476521-5476540 None:intergenic 30.0%
CAATTTCCAGTTGGAAGAAT+CGG - chr2.2:5476171-5476190 MS.gene30946:CDS 35.0%
! TTGTGTTCAGGTTCTTGAAT+TGG - chr2.2:5476367-5476386 MS.gene30946:intron 35.0%
ATCGGTCGTTACTTGAAGAA+AGG - chr2.2:5476189-5476208 MS.gene30946:CDS 40.0%
GAAGCCTTCTCATTCTTCTT+AGG + chr2.2:5476545-5476564 None:intergenic 40.0%
GATATTCAAGAACAGCAGCT+AGG + chr2.2:5476254-5476273 None:intergenic 40.0%
GTGAGGAATGATGAAGAACT+TGG - chr2.2:5476452-5476471 MS.gene30946:CDS 40.0%
TCTTGAATATCTTGCTGCTG+AGG - chr2.2:5476263-5476282 MS.gene30946:CDS 40.0%
! CTAAGCCTTCTTTGGAGATT+TGG + chr2.2:5476597-5476616 None:intergenic 40.0%
AGAACAGCAGCTAGGTAAAC+AGG + chr2.2:5476246-5476265 None:intergenic 45.0%
AGAAGAACAGGATCATCCCT+AGG - chr2.2:5476414-5476433 MS.gene30946:CDS 45.0%
CACGTGACAACAAGAAGAAC+AGG - chr2.2:5476402-5476421 MS.gene30946:intron 45.0%
GAACTTGGAAAGTTGCTAGC+TGG - chr2.2:5476467-5476486 MS.gene30946:CDS 45.0%
GCTGGAGTTACAATTGCACA+TGG - chr2.2:5476485-5476504 MS.gene30946:CDS 45.0%
GGAGTTACAATTGCACATGG+TGG - chr2.2:5476488-5476507 MS.gene30946:CDS 45.0%
GGCTACCAAATCTCCAAAGA+AGG - chr2.2:5476589-5476608 MS.gene30946:CDS 45.0%
TGCTACCAAATCTCCATCCA+AGG - chr2.2:5476568-5476587 MS.gene30946:CDS 45.0%
TGGAAATTGAAGACCAGCTC+TGG + chr2.2:5476160-5476179 None:intergenic 45.0%
! AAATTGAAGACCAGCTCTGG+TGG + chr2.2:5476157-5476176 None:intergenic 45.0%
! CTTTGGAGATTTGGTAGCCT+TGG + chr2.2:5476588-5476607 None:intergenic 45.0%
! GTTCAGGTTCTTGAATTGGC+TGG - chr2.2:5476371-5476390 MS.gene30946:intron 45.0%
! TAACGACCGATTCTTCCAAC+TGG + chr2.2:5476180-5476199 None:intergenic 45.0%
!! GCTGGTCTTCAATTTCCAGT+TGG - chr2.2:5476162-5476181 MS.gene30946:CDS 45.0%
ACTGCCAACAGCAAATGCCT+AGG + chr2.2:5476434-5476453 None:intergenic 50.0%
CTGCCAACAGCAAATGCCTA+GGG + chr2.2:5476433-5476452 None:intergenic 50.0%
CTTGTCACTGCCTTCTTCCT+TGG + chr2.2:5476132-5476151 None:intergenic 50.0%
GAAAGGAGGTCCAAGGAAGA+AGG - chr2.2:5476119-5476138 MS.gene30946:CDS 50.0%
GCAAGCACCAAGACGAAGAA+GGG - chr2.2:5476084-5476103 MS.gene30946:CDS 50.0%
! GGAGATTTGGTAGCCTTGGA+TGG + chr2.2:5476584-5476603 None:intergenic 50.0%
! GGTAGCCTTGGATGGAGATT+TGG + chr2.2:5476576-5476595 None:intergenic 50.0%
!! CATCCCTAGGCATTTGCTGT+TGG - chr2.2:5476427-5476446 MS.gene30946:CDS 50.0%
AAGACGAAGAAGGGAGCTGG+TGG - chr2.2:5476093-5476112 MS.gene30946:CDS 55.0%
AAGGGAGCTGGTGGAAGGAA+AGG - chr2.2:5476102-5476121 MS.gene30946:CDS 55.0%
ACCAAGACGAAGAAGGGAGC+TGG - chr2.2:5476090-5476109 MS.gene30946:CDS 55.0%
ACCAGCTCCCTTCTTCGTCT+TGG + chr2.2:5476094-5476113 None:intergenic 55.0%
CGCAAGCACCAAGACGAAGA+AGG - chr2.2:5476083-5476102 MS.gene30946:CDS 55.0%
GGAAGATACGCTCAGCGTGT+TGG - chr2.2:5476210-5476229 MS.gene30946:CDS 55.0%
GTGACAAGATCCACCAGAGC+TGG - chr2.2:5476144-5476163 MS.gene30946:CDS 55.0%
GTGGAAGGAAAGGAGGTCCA+AGG - chr2.2:5476112-5476131 MS.gene30946:CDS 55.0%
! TACGCTCAGCGTGTTGGTAC+TGG - chr2.2:5476216-5476235 MS.gene30946:CDS 55.0%
!! GGCATTTGCTGTTGGCAGTG+AGG - chr2.2:5476435-5476454 MS.gene30946:CDS 55.0%
CGAAGAAGGGAGCTGGTGGA+AGG - chr2.2:5476097-5476116 MS.gene30946:CDS 60.0%
GGAGCTGGTGGAAGGAAAGG+AGG - chr2.2:5476105-5476124 MS.gene30946:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5476078 5476616 5476078 ID=MS.gene30946
chr2.2 mRNA 5476078 5476616 5476078 ID=MS.gene30946.t1;Parent=MS.gene30946
chr2.2 exon 5476410 5476616 5476410 ID=MS.gene30946.t1.exon1;Parent=MS.gene30946.t1
chr2.2 CDS 5476410 5476616 5476410 ID=cds.MS.gene30946.t1;Parent=MS.gene30946.t1
chr2.2 exon 5476078 5476317 5476078 ID=MS.gene30946.t1.exon2;Parent=MS.gene30946.t1
chr2.2 CDS 5476078 5476317 5476078 ID=cds.MS.gene30946.t1;Parent=MS.gene30946.t1
Gene Sequence

>MS.gene30946

ATGGACGCAAGCACCAAGACGAAGAAGGGAGCTGGTGGAAGGAAAGGAGGTCCAAGGAAGAAGGCAGTGACAAGATCCACCAGAGCTGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGTTACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGTACTGGTGCTCCTGTTTACCTAGCTGCTGTTCTTGAATATCTTGCTGCTGAGGTATATCTCAATTTCAATTTTCCCTCTTTTGATTTTCAAATTTGAAATTCAAACCCTAATTTTGATGAAATTGAAATTTGATTTGTGTTCAGGTTCTTGAATTGGCTGGAAATGCTGCACGTGACAACAAGAAGAACAGGATCATCCCTAGGCATTTGCTGTTGGCAGTGAGGAATGATGAAGAACTTGGAAAGTTGCTAGCTGGAGTTACAATTGCACATGGTGGTGTTCTTCCAAACATCAACCCAATTCTTTTACCTAAGAAGAATGAGAAGGCTTCAACTGCTACCAAATCTCCATCCAAGGCTACCAAATCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene30946.t1

MDASTKTKKGAGGRKGGPRKKAVTRSTRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKNEKASTATKSPSKATKSPKKA