Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37238.t1 | RHN50250.1 | 98.6 | 71 | 1 | 0 | 1 | 71 | 143 | 213 | 2.90E-30 | 140.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37238.t1 | A0A072U6X6 | 98.6 | 71 | 1 | 0 | 1 | 71 | 76 | 146 | 2.1e-30 | 140.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049756 | MS.gene37238 | 0.80081 | 1.21E-48 | -1.69E-46 |
| MS.gene050084 | MS.gene37238 | 0.849669 | 2.79E-60 | -1.69E-46 |
| MS.gene050085 | MS.gene37238 | 0.874109 | 8.73E-68 | -1.69E-46 |
| MS.gene050114 | MS.gene37238 | 0.809242 | 2.08E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene37238 | 0.808335 | 3.26E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene37238 | 0.809535 | 1.80E-50 | -1.69E-46 |
| MS.gene050320 | MS.gene37238 | 0.801014 | 1.10E-48 | -1.69E-46 |
| MS.gene050359 | MS.gene37238 | 0.815711 | 8.04E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene37238 | 0.80643 | 8.26E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene37238 | 0.807339 | 5.31E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene37238 | 0.819955 | 8.85E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene37238 | 0.854836 | 9.44E-62 | -1.69E-46 |
| MS.gene050945 | MS.gene37238 | 0.819509 | 1.12E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene37238 | 0.840872 | 6.69E-58 | -1.69E-46 |
| MS.gene051233 | MS.gene37238 | 0.831464 | 1.64E-55 | -1.69E-46 |
| MS.gene051234 | MS.gene37238 | 0.829164 | 6.00E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene37238 | 0.845853 | 3.13E-59 | -1.69E-46 |
| MS.gene051381 | MS.gene37238 | 0.805775 | 1.13E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene37238 | 0.807002 | 6.25E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene37238 | 0.81859 | 1.81E-52 | -1.69E-46 |
| MS.gene05188 | MS.gene37238 | 0.81951 | 1.12E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene37238 | 0.83519 | 1.94E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene37238 | 0.816959 | 4.23E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene37238 | 0.824439 | 8.07E-54 | -1.69E-46 |
| MS.gene052072 | MS.gene37238 | 0.819838 | 9.41E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene37238 | -0.848135 | 7.43E-60 | -1.69E-46 |
| MS.gene052335 | MS.gene37238 | 0.802456 | 5.56E-49 | -1.69E-46 |
| MS.gene052342 | MS.gene37238 | 0.836427 | 9.42E-57 | -1.69E-46 |
| MS.gene052434 | MS.gene37238 | 0.815266 | 1.01E-51 | -1.69E-46 |
| MS.gene052479 | MS.gene37238 | 0.805084 | 1.58E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene37238 | 0.818018 | 2.44E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene37238 | 0.878648 | 2.37E-69 | -1.69E-46 |
| MS.gene053109 | MS.gene37238 | 0.813835 | 2.09E-51 | -1.69E-46 |
| MS.gene053167 | MS.gene37238 | 0.810336 | 1.21E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene37238 | 0.823804 | 1.14E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene37238 | 0.81869 | 1.72E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene37238 | 0.825006 | 5.93E-54 | -1.69E-46 |
| MS.gene055314 | MS.gene37238 | 0.816078 | 6.65E-52 | -1.69E-46 |
| MS.gene055424 | MS.gene37238 | 0.803244 | 3.82E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene37238 | 0.814268 | 1.68E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene37238 | 0.827032 | 1.96E-54 | -1.69E-46 |
| MS.gene056097 | MS.gene37238 | 0.83192 | 1.27E-55 | -1.69E-46 |
| MS.gene056181 | MS.gene37238 | 0.834109 | 3.63E-56 | -1.69E-46 |
| MS.gene056403 | MS.gene37238 | 0.811375 | 7.23E-51 | -1.69E-46 |
| MS.gene056580 | MS.gene37238 | 0.815586 | 8.57E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene37238 | 0.830399 | 3.00E-55 | -1.69E-46 |
| MS.gene056752 | MS.gene37238 | 0.803902 | 2.79E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene37238 | 0.82432 | 8.60E-54 | -1.69E-46 |
| MS.gene056849 | MS.gene37238 | 0.815357 | 9.64E-52 | -1.69E-46 |
| MS.gene05689 | MS.gene37238 | 0.802272 | 6.07E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene37238 | 0.804696 | 1.91E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene37238 | 0.829166 | 5.99E-55 | -1.69E-46 |
| MS.gene057234 | MS.gene37238 | 0.820514 | 6.59E-53 | -1.69E-46 |
| MS.gene057452 | MS.gene37238 | -0.819912 | 9.05E-53 | -1.69E-46 |
| MS.gene057705 | MS.gene37238 | 0.810573 | 1.08E-50 | -1.69E-46 |
| MS.gene05781 | MS.gene37238 | 0.848027 | 7.96E-60 | -1.69E-46 |
| MS.gene057890 | MS.gene37238 | -0.81779 | 2.75E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene37238 | 0.828348 | 9.45E-55 | -1.69E-46 |
| MS.gene058054 | MS.gene37238 | 0.859575 | 3.76E-63 | -1.69E-46 |
| MS.gene058055 | MS.gene37238 | 0.832067 | 1.17E-55 | -1.69E-46 |
| MS.gene058074 | MS.gene37238 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene37238 | 0.830494 | 2.85E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene37238 | 0.81229 | 4.57E-51 | -1.69E-46 |
| MS.gene058638 | MS.gene37238 | 0.819938 | 8.93E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene37238 | 0.827603 | 1.43E-54 | -1.69E-46 |
| MS.gene059007 | MS.gene37238 | 0.825744 | 3.97E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene37238 | 0.850049 | 2.18E-60 | -1.69E-46 |
| MS.gene059171 | MS.gene37238 | 0.835702 | 1.44E-56 | -1.69E-46 |
| MS.gene05978 | MS.gene37238 | 0.804361 | 2.24E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene37238 | 0.810817 | 9.54E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene37238 | 0.82648 | 2.65E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene37238 | 0.826469 | 2.67E-54 | -1.69E-46 |
| MS.gene060296 | MS.gene37238 | 0.810071 | 1.38E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene37238 | 0.801829 | 7.48E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene37238 | 0.849262 | 3.62E-60 | -1.69E-46 |
| MS.gene060869 | MS.gene37238 | 0.801419 | 9.09E-49 | -1.69E-46 |
| MS.gene060870 | MS.gene37238 | 0.806553 | 7.78E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene37238 | 0.817007 | 4.12E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene37238 | 0.801048 | 1.08E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37238.t1 | MTR_6g015885 | 98.592 | 71 | 1 | 0 | 1 | 71 | 76 | 146 | 2.97e-42 | 144 |
| MS.gene37238.t1 | MTR_6g015885 | 98.592 | 71 | 1 | 0 | 1 | 71 | 134 | 204 | 3.57e-42 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTTGACTTATGATCTTC+TGG | 0.209624 | 6.3:-10778008 | None:intergenic |
| CTCGGTGGCTCCAGCTCTGC+CGG | 0.390513 | 6.3:-10778155 | None:intergenic |
| CCAGAAGATCATAAGTCAAA+TGG | 0.427035 | 6.3:+10778008 | MS.gene37238:CDS |
| TGAAGAGCAAGTTGATGATT+CGG | 0.448596 | 6.3:+10778121 | MS.gene37238:CDS |
| TTAGCGGAAGAGACATCTCC+TGG | 0.459743 | 6.3:+10778041 | MS.gene37238:CDS |
| TGATTCTTCTGAAATGGTTG+TGG | 0.527746 | 6.3:+10777962 | None:intergenic |
| TTTGACTTATGATCTTCTGG+TGG | 0.528551 | 6.3:-10778005 | None:intergenic |
| ATTGTAGTGCATGATTCTCC+AGG | 0.531662 | 6.3:-10778059 | None:intergenic |
| AAATGGAAAATGTGAGTTAG+CGG | 0.567232 | 6.3:+10778025 | MS.gene37238:CDS |
| GGAGCCACCGAGGGTTCTTG+AGG | 0.571951 | 6.3:+10778166 | MS.gene37238:CDS |
| TGGAGAATCATGCACTACAA+TGG | 0.582816 | 6.3:+10778061 | MS.gene37238:CDS |
| GAATCATGCACTACAATGGA+AGG | 0.591686 | 6.3:+10778065 | MS.gene37238:CDS |
| GGCAGAGCTGGAGCCACCGA+GGG | 0.604326 | 6.3:+10778157 | MS.gene37238:CDS |
| TTCTTCTGAAATGGTTGTGG+AGG | 0.613449 | 6.3:+10777965 | None:intergenic |
| GAGCAAGTTGATGATTCGGA+AGG | 0.620607 | 6.3:+10778125 | MS.gene37238:CDS |
| GTCAAGATCGATGAAGAATG+TGG | 0.653077 | 6.3:+10778092 | MS.gene37238:CDS |
| CGGCAGAGCTGGAGCCACCG+AGG | 0.657470 | 6.3:+10778156 | MS.gene37238:CDS |
| GGAACCTCAAGAACCCTCGG+TGG | 0.759107 | 6.3:-10778170 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGGAAAATGTGAGTTAG+CGG | + | chr6.3:10778025-10778044 | MS.gene37238:CDS | 30.0% | |
| CCAGAAGATCATAAGTCAAA+TGG | + | chr6.3:10778008-10778027 | MS.gene37238:CDS | 35.0% | |
| ! | CCATTTGACTTATGATCTTC+TGG | - | chr6.3:10778011-10778030 | None:intergenic | 35.0% |
| ! | TTTGACTTATGATCTTCTGG+TGG | - | chr6.3:10778008-10778027 | None:intergenic | 35.0% |
| !! | TGAAGAGCAAGTTGATGATT+CGG | + | chr6.3:10778121-10778140 | MS.gene37238:CDS | 35.0% |
| ATTGTAGTGCATGATTCTCC+AGG | - | chr6.3:10778062-10778081 | None:intergenic | 40.0% | |
| GAATCATGCACTACAATGGA+AGG | + | chr6.3:10778065-10778084 | MS.gene37238:CDS | 40.0% | |
| GTCAAGATCGATGAAGAATG+TGG | + | chr6.3:10778092-10778111 | MS.gene37238:CDS | 40.0% | |
| TGGAGAATCATGCACTACAA+TGG | + | chr6.3:10778061-10778080 | MS.gene37238:CDS | 40.0% | |
| ! | TGATTCGGAAGGAAAAAGAC+CGG | + | chr6.3:10778136-10778155 | MS.gene37238:CDS | 40.0% |
| ATCAACTTCCTCCACAACCA+TGG | - | chr6.3:10777976-10777995 | None:intergenic | 45.0% | |
| !! | GAGCAAGTTGATGATTCGGA+AGG | + | chr6.3:10778125-10778144 | MS.gene37238:CDS | 45.0% |
| TTAGCGGAAGAGACATCTCC+TGG | + | chr6.3:10778041-10778060 | MS.gene37238:CDS | 50.0% | |
| AGGAAAAAGACCGGCAGAGC+TGG | + | chr6.3:10778145-10778164 | MS.gene37238:CDS | 55.0% | |
| GGAACCTCAAGAACCCTCGG+TGG | - | chr6.3:10778173-10778192 | None:intergenic | 60.0% | |
| GGAGCCACCGAGGGTTCTTG+AGG | + | chr6.3:10778166-10778185 | MS.gene37238:CDS | 65.0% | |
| CTCGGTGGCTCCAGCTCTGC+CGG | - | chr6.3:10778158-10778177 | None:intergenic | 70.0% | |
| GGCAGAGCTGGAGCCACCGA+GGG | + | chr6.3:10778157-10778176 | MS.gene37238:CDS | 70.0% | |
| CGGCAGAGCTGGAGCCACCG+AGG | + | chr6.3:10778156-10778175 | MS.gene37238:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 10777975 | 10778192 | 10777975 | ID=MS.gene37238 |
| chr6.3 | mRNA | 10777975 | 10778192 | 10777975 | ID=MS.gene37238.t1;Parent=MS.gene37238 |
| chr6.3 | exon | 10777975 | 10778192 | 10777975 | ID=MS.gene37238.t1.exon1;Parent=MS.gene37238.t1 |
| chr6.3 | CDS | 10777975 | 10778192 | 10777975 | ID=cds.MS.gene37238.t1;Parent=MS.gene37238.t1 |
| Gene Sequence |
| Protein sequence |
