Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40205.t1 | GAU14324.1 | 84.1 | 88 | 12 | 2 | 4 | 90 | 1 | 87 | 2.30E-32 | 147.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40205.t1 | G7J4L6 | 83.9 | 87 | 8 | 1 | 4 | 90 | 1 | 81 | 1.1e-31 | 145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene40205 | 0.81473 | 1.33E-51 | -1.69E-46 |
| MS.gene049369 | MS.gene40205 | 0.80166 | 8.11E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene40205 | 0.836711 | 7.97E-57 | -1.69E-46 |
| MS.gene049633 | MS.gene40205 | 0.817758 | 2.79E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene40205 | 0.832452 | 9.38E-56 | -1.69E-46 |
| MS.gene050432 | MS.gene40205 | 0.813274 | 2.78E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene40205 | 0.836012 | 1.20E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene40205 | 0.810986 | 8.78E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene40205 | 0.825244 | 5.21E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene40205 | 0.84081 | 6.95E-58 | -1.69E-46 |
| MS.gene05080 | MS.gene40205 | 0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene40205 | 0.823018 | 1.74E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene40205 | 0.811443 | 6.98E-51 | -1.69E-46 |
| MS.gene051228 | MS.gene40205 | 0.830586 | 2.70E-55 | -1.69E-46 |
| MS.gene051617 | MS.gene40205 | 0.817086 | 3.96E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene40205 | 0.830058 | 3.64E-55 | -1.69E-46 |
| MS.gene051838 | MS.gene40205 | 0.826241 | 3.02E-54 | -1.69E-46 |
| MS.gene052134 | MS.gene40205 | 0.813904 | 2.02E-51 | -1.69E-46 |
| MS.gene052135 | MS.gene40205 | 0.813472 | 2.52E-51 | -1.69E-46 |
| MS.gene052136 | MS.gene40205 | 0.82506 | 5.76E-54 | -1.69E-46 |
| MS.gene052414 | MS.gene40205 | 0.800924 | 1.15E-48 | -1.69E-46 |
| MS.gene052421 | MS.gene40205 | 0.807127 | 5.88E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene40205 | 0.804946 | 1.69E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene40205 | 0.80471 | 1.90E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene40205 | 0.806049 | 9.93E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene40205 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene40205 | 0.8154 | 9.43E-52 | -1.69E-46 |
| MS.gene052872 | MS.gene40205 | 0.816407 | 5.62E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene40205 | 0.80797 | 3.90E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene40205 | 0.82529 | 5.08E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene40205 | 0.831524 | 1.59E-55 | -1.69E-46 |
| MS.gene053207 | MS.gene40205 | 0.813814 | 2.12E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene40205 | 0.80333 | 3.67E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene40205 | 0.847146 | 1.39E-59 | -1.69E-46 |
| MS.gene053574 | MS.gene40205 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene40205 | 0.808019 | 3.80E-50 | -1.69E-46 |
| MS.gene053824 | MS.gene40205 | 0.821249 | 4.46E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene40205 | 0.836787 | 7.62E-57 | -1.69E-46 |
| MS.gene053916 | MS.gene40205 | 0.84086 | 6.74E-58 | -1.69E-46 |
| MS.gene054642 | MS.gene40205 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene40205 | 0.803675 | 3.11E-49 | -1.69E-46 |
| MS.gene054955 | MS.gene40205 | 0.801503 | 8.73E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene40205 | 0.83054 | 2.77E-55 | -1.69E-46 |
| MS.gene055206 | MS.gene40205 | 0.831159 | 1.95E-55 | -1.69E-46 |
| MS.gene055224 | MS.gene40205 | 0.816078 | 6.66E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene40205 | 0.838661 | 2.52E-57 | -1.69E-46 |
| MS.gene055577 | MS.gene40205 | 0.800097 | 1.69E-48 | -1.69E-46 |
| MS.gene055722 | MS.gene40205 | 0.84447 | 7.41E-59 | -1.69E-46 |
| MS.gene05592 | MS.gene40205 | 0.842737 | 2.15E-58 | -1.69E-46 |
| MS.gene056511 | MS.gene40205 | 0.849463 | 3.18E-60 | -1.69E-46 |
| MS.gene056609 | MS.gene40205 | 0.804199 | 2.42E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene40205 | 0.811549 | 6.62E-51 | -1.69E-46 |
| MS.gene05666 | MS.gene40205 | 0.807636 | 4.59E-50 | -1.69E-46 |
| MS.gene056897 | MS.gene40205 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene40205 | 0.820179 | 7.86E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene40205 | 0.813653 | 2.30E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene40205 | 0.817593 | 3.04E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene40205 | 0.81023 | 1.28E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene40205 | 0.833783 | 4.37E-56 | -1.69E-46 |
| MS.gene05735 | MS.gene40205 | 0.821592 | 3.72E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene40205 | 0.828109 | 1.08E-54 | -1.69E-46 |
| MS.gene057734 | MS.gene40205 | 0.828156 | 1.05E-54 | -1.69E-46 |
| MS.gene057856 | MS.gene40205 | 0.811529 | 6.69E-51 | -1.69E-46 |
| MS.gene058006 | MS.gene40205 | 0.808971 | 2.38E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene40205 | 0.810232 | 1.28E-50 | -1.69E-46 |
| MS.gene058723 | MS.gene40205 | 0.816013 | 6.88E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene40205 | 0.816051 | 6.75E-52 | -1.69E-46 |
| MS.gene059305 | MS.gene40205 | 0.802278 | 6.05E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene40205 | 0.809414 | 1.91E-50 | -1.69E-46 |
| MS.gene059536 | MS.gene40205 | 0.800617 | 1.33E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene40205 | 0.811854 | 5.68E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene40205 | 0.802797 | 4.73E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene40205 | 0.821717 | 3.48E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene40205 | 0.800968 | 1.12E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene40205 | 0.827767 | 1.30E-54 | -1.69E-46 |
| MS.gene060818 | MS.gene40205 | 0.85878 | 6.51E-63 | -1.69E-46 |
| MS.gene060835 | MS.gene40205 | 0.828964 | 6.71E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene40205 | 0.817292 | 3.56E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene40205 | 0.817409 | 3.35E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene40205 | 0.830613 | 2.66E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40205.t1 | MTR_3g111360 | 83.908 | 87 | 8 | 1 | 4 | 90 | 1 | 81 | 1.78e-46 | 143 |
| MS.gene40205.t1 | MTR_5g067050 | 64.286 | 70 | 21 | 3 | 22 | 89 | 13 | 80 | 7.02e-23 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGGGTTGCTTTCATGCTT+TGG | 0.283837 | 3.2:-89053686 | MS.gene40205:CDS |
| TTAGCTAGCAATGGAAAGTT+TGG | 0.296785 | 3.2:-89053750 | None:intergenic |
| GAGGGTTGCTTTCATGCTTT+GGG | 0.333399 | 3.2:-89053685 | MS.gene40205:CDS |
| TTGTTTGTTCTCCTCTTCCA+TGG | 0.436490 | 3.2:+89053644 | None:intergenic |
| GAGAACGACGAAGAATCTTT+AGG | 0.441691 | 3.2:-89053545 | MS.gene40205:CDS |
| TCTCCTATAAGAAGCAAAAC+AGG | 0.462208 | 3.2:-89053713 | MS.gene40205:CDS |
| AGAAGCAAAACAGGTGGAGG+AGG | 0.521376 | 3.2:-89053704 | MS.gene40205:CDS |
| AACACTTATAGAACCGTTGG+TGG | 0.530511 | 3.2:-89053623 | MS.gene40205:CDS |
| AATTGAAGCTTCCATGGAAG+AGG | 0.536567 | 3.2:-89053655 | MS.gene40205:CDS |
| AACAACACTTATAGAACCGT+TGG | 0.561342 | 3.2:-89053626 | MS.gene40205:CDS |
| GTCTCAAATTGAAGCTTCCA+TGG | 0.569556 | 3.2:-89053661 | MS.gene40205:CDS |
| ATAAGAAGCAAAACAGGTGG+AGG | 0.576069 | 3.2:-89053707 | MS.gene40205:CDS |
| GACGAAGAATCTTTAGGTCG+CGG | 0.579606 | 3.2:-89053539 | MS.gene40205:CDS |
| ATCATACCTAAAACCACCAA+CGG | 0.621720 | 3.2:+89053610 | None:intergenic |
| TTGCTTCTTATAGGAGACGA+TGG | 0.630393 | 3.2:+89053719 | None:intergenic |
| GAAGCAAAACAGGTGGAGGA+GGG | 0.645020 | 3.2:-89053703 | MS.gene40205:CDS |
| CCTATAAGAAGCAAAACAGG+TGG | 0.657762 | 3.2:-89053710 | MS.gene40205:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGATGATGGTAATT+TGG | - | chr3.2:89053660-89053679 | MS.gene40205:CDS | 20.0% |
| !! | AAATTTTGAGCATAGCTTAA+AGG | + | chr3.2:89053646-89053665 | None:intergenic | 25.0% |
| !!! | TATGCTCAAAATTTTGATGA+TGG | - | chr3.2:89053652-89053671 | MS.gene40205:CDS | 25.0% |
| !!! | TTTTGATGATGGTAATTTGG+AGG | - | chr3.2:89053663-89053682 | MS.gene40205:CDS | 30.0% |
| AACAACACTTATAGAACCGT+TGG | - | chr3.2:89053604-89053623 | MS.gene40205:CDS | 35.0% | |
| ATCATACCTAAAACCACCAA+CGG | + | chr3.2:89053623-89053642 | None:intergenic | 35.0% | |
| TCTCCTATAAGAAGCAAAAC+AGG | - | chr3.2:89053517-89053536 | MS.gene40205:CDS | 35.0% | |
| AACACTTATAGAACCGTTGG+TGG | - | chr3.2:89053607-89053626 | MS.gene40205:CDS | 40.0% | |
| AATTGAAGCTTCCATGGAAG+AGG | - | chr3.2:89053575-89053594 | MS.gene40205:CDS | 40.0% | |
| ATAAGAAGCAAAACAGGTGG+AGG | - | chr3.2:89053523-89053542 | MS.gene40205:CDS | 40.0% | |
| CCTATAAGAAGCAAAACAGG+TGG | - | chr3.2:89053520-89053539 | MS.gene40205:CDS | 40.0% | |
| GAGAACGACGAAGAATCTTT+AGG | - | chr3.2:89053685-89053704 | MS.gene40205:CDS | 40.0% | |
| GTCTCAAATTGAAGCTTCCA+TGG | - | chr3.2:89053569-89053588 | MS.gene40205:CDS | 40.0% | |
| TTGCTTCTTATAGGAGACGA+TGG | + | chr3.2:89053514-89053533 | None:intergenic | 40.0% | |
| TTGTTTGTTCTCCTCTTCCA+TGG | + | chr3.2:89053589-89053608 | None:intergenic | 40.0% | |
| ! | CCACCTGTTTTGCTTCTTAT+AGG | + | chr3.2:89053523-89053542 | None:intergenic | 40.0% |
| !!! | ATAGAACCGTTGGTGGTTTT+AGG | - | chr3.2:89053614-89053633 | MS.gene40205:CDS | 40.0% |
| !!! | TGCTTGAGTGTTTTAGCTGA+AGG | + | chr3.2:89053739-89053758 | None:intergenic | 40.0% |
| GACGAAGAATCTTTAGGTCG+CGG | - | chr3.2:89053691-89053710 | MS.gene40205:CDS | 45.0% | |
| ! | GAGGGTTGCTTTCATGCTTT+GGG | - | chr3.2:89053545-89053564 | MS.gene40205:CDS | 45.0% |
| AGAAGCAAAACAGGTGGAGG+AGG | - | chr3.2:89053526-89053545 | MS.gene40205:CDS | 50.0% | |
| GAAGCAAAACAGGTGGAGGA+GGG | - | chr3.2:89053527-89053546 | MS.gene40205:CDS | 50.0% | |
| ! | GGAGGGTTGCTTTCATGCTT+TGG | - | chr3.2:89053544-89053563 | MS.gene40205:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 89053490 | 89053762 | 89053490 | ID=MS.gene40205 |
| chr3.2 | mRNA | 89053490 | 89053762 | 89053490 | ID=MS.gene40205.t1;Parent=MS.gene40205 |
| chr3.2 | exon | 89053490 | 89053762 | 89053490 | ID=MS.gene40205.t1.exon1;Parent=MS.gene40205.t1 |
| chr3.2 | CDS | 89053490 | 89053762 | 89053490 | ID=cds.MS.gene40205.t1;Parent=MS.gene40205.t1 |
| Gene Sequence |
| Protein sequence |
