AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45403


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45403.t1 MTR_4g088515 97.015 268 8 0 1 268 1 268 0.0 541
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45403.t1 AT4G26415 45.299 234 61 10 3 192 7 217 3.13e-44 152

Find 59 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCCTCAAGAACTCTT+TGG 0.228130 4.4:+24350247 MS.gene45403:CDS
TCAATGAATATAGTGGAATA+AGG 0.272127 4.4:+24350516 MS.gene45403:CDS
TGGTGTTGTGGCAAAGTTAA+TGG 0.280238 4.4:+24350772 MS.gene45403:CDS
GCCATTTGAGAGGCATGATT+TGG 0.298125 4.4:+24350892 MS.gene45403:CDS
TCCATGTATAGGTCATCTTT+TGG 0.313265 4.4:+24350632 MS.gene45403:CDS
TCTTATGCCTTTCTTCATCT+TGG 0.319608 4.4:-24350273 None:intergenic
TTTCTCCTCAAGAACTCTTT+GGG 0.349291 4.4:+24350248 MS.gene45403:CDS
AACCAACAAAATAGTACTAT+TGG 0.366556 4.4:+24350332 MS.gene45403:CDS
TGGATATTGTATTATGAAAC+CGG 0.367697 4.4:+24350997 MS.gene45403:CDS
GGATGAGTAGTGTTGATTGT+AGG 0.385703 4.4:+24350849 MS.gene45403:CDS
CAATGAATATAGTGGAATAA+GGG 0.392950 4.4:+24350517 MS.gene45403:CDS
AGAACCAAAGCTTGATGAAA+AGG 0.407802 4.4:+24350697 MS.gene45403:CDS
GCCATAGCTTCTAGAGAAAC+CGG 0.424661 4.4:-24351016 None:intergenic
GGTGTTGTGGCAAAGTTAAT+GGG 0.432813 4.4:+24350773 MS.gene45403:CDS
TGTGGCAAAGTTAATGGGTT+TGG 0.433784 4.4:+24350778 MS.gene45403:CDS
TCTTATACTACAATTAGAAA+TGG 0.443850 4.4:+24350466 MS.gene45403:CDS
CTCCTTGGTTGCCAACTACT+AGG 0.453422 4.4:-24351046 None:intergenic
GTTAGAAGAAGAAATAGGTC+TGG 0.472977 4.4:+24350959 MS.gene45403:CDS
AGTATAAGATCTTCTAATGT+TGG 0.477524 4.4:-24350452 None:intergenic
TCCAAATCATGCCTCTCAAA+TGG 0.478781 4.4:-24350893 None:intergenic
TGCTGCTTGTGATGTCAACT+TGG 0.485632 4.4:-24350376 None:intergenic
AGTACTATTGGCAATGGCAT+TGG 0.489373 4.4:+24350344 MS.gene45403:CDS
CTAATCTAAACATATCTCCT+TGG 0.491234 4.4:-24351061 None:intergenic
AAAACTAGCAATGGACATGC+AGG 0.491726 4.4:+24350919 MS.gene45403:CDS
CCCAAAAGATGACCTATACA+TGG 0.498041 4.4:-24350633 None:intergenic
TTGTGATGTCAACTTGGCTA+TGG 0.503039 4.4:-24350370 None:intergenic
TTCTCCCCAGCAACTGTTGG+TGG 0.503702 4.4:-24350731 None:intergenic
GTAGGAGACAAAATATAAGA+AGG 0.511223 4.4:+24350867 MS.gene45403:CDS
ATGGAAGAGATTCCATGTAT+AGG 0.512387 4.4:+24350621 MS.gene45403:CDS
ATTAACTTTGCCACAACACC+AGG 0.513967 4.4:-24350770 None:intergenic
TGGCAGGAGGTCCTAGTAGT+TGG 0.521625 4.4:+24351035 MS.gene45403:CDS
ACCGGTTTCTCTAGAAGCTA+TGG 0.522607 4.4:+24351015 MS.gene45403:CDS
TTGCTGGGGAGAACGTGGTT+TGG 0.527912 4.4:+24350741 MS.gene45403:CDS
ACATTGTTAGAAGAAGAAAT+AGG 0.529346 4.4:+24350954 MS.gene45403:CDS
ATGCTTTGAATCAATATCCA+AGG 0.531501 4.4:+24350588 MS.gene45403:CDS
AAATGGAGGAAGAGATGGCA+AGG 0.533678 4.4:+24350483 MS.gene45403:CDS
ATTAGAAATGGAGGAAGAGA+TGG 0.541356 4.4:+24350478 MS.gene45403:CDS
GAGTTCTTGAGGAGAAAGAA+TGG 0.549925 4.4:-24350242 None:intergenic
TTGGGAGCCAAGATGAAGAA+AGG 0.561593 4.4:+24350266 MS.gene45403:CDS
CAAAATAGTACTATTGGCAA+TGG 0.563916 4.4:+24350338 MS.gene45403:CDS
CTTCCATCAAGTGAAAACCT+TGG 0.575355 4.4:-24350605 None:intergenic
GTCCTAGTAGTTGGCAACCA+AGG 0.577078 4.4:+24351044 MS.gene45403:CDS
AATGAATATAGTGGAATAAG+GGG 0.579962 4.4:+24350518 MS.gene45403:CDS
TATACTACAATTAGAAATGG+AGG 0.596710 4.4:+24350469 MS.gene45403:CDS
TGGCTCCCAAAGAGTTCTTG+AGG 0.597986 4.4:-24350253 None:intergenic
GAAGCTATTCCAGTGTCAGT+AGG 0.605402 4.4:+24350800 MS.gene45403:CDS
TTTGGAGAGAAAACTAGCAA+TGG 0.605568 4.4:+24350910 MS.gene45403:CDS
AACGTGGTTTGGTGTAAACC+TGG 0.608504 4.4:+24350752 MS.gene45403:CDS
GTTTCTCTAGAAGCTATGGC+AGG 0.610709 4.4:+24351019 MS.gene45403:CDS
AACAGTTGCTGGGGAGAACG+TGG 0.612915 4.4:+24350736 MS.gene45403:CDS
AATATAGTGGAATAAGGGGA+AGG 0.620558 4.4:+24350522 MS.gene45403:CDS
GGAGACAAAATATAAGAAGG+AGG 0.626425 4.4:+24350870 MS.gene45403:CDS
TAAGAAGGAGGCCATTTGAG+AGG 0.627512 4.4:+24350882 MS.gene45403:CDS
CTGACATATTAAGATCTGCA+AGG 0.639107 4.4:+24350564 MS.gene45403:CDS
AGTTGACATCACAAGCAGCA+AGG 0.656859 4.4:+24350379 MS.gene45403:CDS
ACGTTCTCCCCAGCAACTGT+TGG 0.657823 4.4:-24350734 None:intergenic
TCTCTAGAAGCTATGGCAGG+AGG 0.665560 4.4:+24351022 MS.gene45403:CDS
TTGGTGTAAACCTGGTGTTG+TGG 0.669247 4.4:+24350760 MS.gene45403:CDS
AAACTAGCAATGGACATGCA+GGG 0.676573 4.4:+24350920 MS.gene45403:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTATACTACAATTAGAAA+TGG + chr4.4:24350466-24350485 MS.gene45403:CDS 20.0%
! AACCAACAAAATAGTACTAT+TGG + chr4.4:24350332-24350351 MS.gene45403:CDS 25.0%
! ACATTGTTAGAAGAAGAAAT+AGG + chr4.4:24350954-24350973 MS.gene45403:CDS 25.0%
! AGTATAAGATCTTCTAATGT+TGG - chr4.4:24350455-24350474 None:intergenic 25.0%
! GAAAAACTCAATGAATATAG+TGG + chr4.4:24350509-24350528 MS.gene45403:CDS 25.0%
! TATACTACAATTAGAAATGG+AGG + chr4.4:24350469-24350488 MS.gene45403:CDS 25.0%
! TGGATATTGTATTATGAAAC+CGG + chr4.4:24350997-24351016 MS.gene45403:CDS 25.0%
!! AATGAATATAGTGGAATAAG+GGG + chr4.4:24350518-24350537 MS.gene45403:CDS 25.0%
!! CAATGAATATAGTGGAATAA+GGG + chr4.4:24350517-24350536 MS.gene45403:CDS 25.0%
!! TCAATGAATATAGTGGAATA+AGG + chr4.4:24350516-24350535 MS.gene45403:CDS 25.0%
AAAGATCTGACAAAAAAGAG+AGG + chr4.4:24350828-24350847 MS.gene45403:CDS 30.0%
AGTTGTCACATTGCAAAAAA+GGG - chr4.4:24350412-24350431 None:intergenic 30.0%
CAAAATAGTACTATTGGCAA+TGG + chr4.4:24350338-24350357 MS.gene45403:CDS 30.0%
GTAGGAGACAAAATATAAGA+AGG + chr4.4:24350867-24350886 MS.gene45403:CDS 30.0%
! ATAACCTTTTCATCAAGCTT+TGG - chr4.4:24350704-24350723 None:intergenic 30.0%
! ATGCTTTGAATCAATATCCA+AGG + chr4.4:24350588-24350607 MS.gene45403:CDS 30.0%
! TGCCAATAGTACTATTTTGT+TGG - chr4.4:24350337-24350356 None:intergenic 30.0%
!!! ATTTTGTTGGTTTAGAGTTG+AGG - chr4.4:24350324-24350343 None:intergenic 30.0%
!!! TCTGGTTTTTGCTCTAAAAA+TGG + chr4.4:24350977-24350996 MS.gene45403:CDS 30.0%
AGAACCAAAGCTTGATGAAA+AGG + chr4.4:24350697-24350716 MS.gene45403:CDS 35.0%
ATGGAAGAGATTCCATGTAT+AGG + chr4.4:24350621-24350640 MS.gene45403:CDS 35.0%
ATTAGAAATGGAGGAAGAGA+TGG + chr4.4:24350478-24350497 MS.gene45403:CDS 35.0%
CTGACATATTAAGATCTGCA+AGG + chr4.4:24350564-24350583 MS.gene45403:CDS 35.0%
GAGTTGTCACATTGCAAAAA+AGG - chr4.4:24350413-24350432 None:intergenic 35.0%
GGAGACAAAATATAAGAAGG+AGG + chr4.4:24350870-24350889 MS.gene45403:CDS 35.0%
GTTAGAAGAAGAAATAGGTC+TGG + chr4.4:24350959-24350978 MS.gene45403:CDS 35.0%
GTTGTCACATTGCAAAAAAG+GGG - chr4.4:24350411-24350430 None:intergenic 35.0%
TCTTATGCCTTTCTTCATCT+TGG - chr4.4:24350276-24350295 None:intergenic 35.0%
TTTCTCCTCAAGAACTCTTT+GGG + chr4.4:24350248-24350267 MS.gene45403:CDS 35.0%
TTTGGAGAGAAAACTAGCAA+TGG + chr4.4:24350910-24350929 MS.gene45403:CDS 35.0%
! AATATAGTGGAATAAGGGGA+AGG + chr4.4:24350522-24350541 MS.gene45403:CDS 35.0%
! CCATGTATAGGTCATCTTTT+GGG + chr4.4:24350633-24350652 MS.gene45403:CDS 35.0%
! TATCCAAGGTTTTCACTTGA+TGG + chr4.4:24350602-24350621 MS.gene45403:CDS 35.0%
! TCCATGTATAGGTCATCTTT+TGG + chr4.4:24350632-24350651 MS.gene45403:CDS 35.0%
!!! TCATCTTTTGGGACTATAGA+AGG + chr4.4:24350644-24350663 MS.gene45403:CDS 35.0%
AAAACTAGCAATGGACATGC+AGG + chr4.4:24350919-24350938 MS.gene45403:CDS 40.0%
AAACTAGCAATGGACATGCA+GGG + chr4.4:24350920-24350939 MS.gene45403:CDS 40.0%
AGTACTATTGGCAATGGCAT+TGG + chr4.4:24350344-24350363 MS.gene45403:CDS 40.0%
ATTAACTTTGCCACAACACC+AGG - chr4.4:24350773-24350792 None:intergenic 40.0%
CCCAAAAGATGACCTATACA+TGG - chr4.4:24350636-24350655 None:intergenic 40.0%
CTTCCATCAAGTGAAAACCT+TGG - chr4.4:24350608-24350627 None:intergenic 40.0%
CTTTCTCCTCAAGAACTCTT+TGG + chr4.4:24350247-24350266 MS.gene45403:CDS 40.0%
GAGTTCTTGAGGAGAAAGAA+TGG - chr4.4:24350245-24350264 None:intergenic 40.0%
TCCAAATCATGCCTCTCAAA+TGG - chr4.4:24350896-24350915 None:intergenic 40.0%
TTGTGATGTCAACTTGGCTA+TGG - chr4.4:24350373-24350392 None:intergenic 40.0%
! CTTTTTCTACCTACTGACAC+TGG - chr4.4:24350812-24350831 None:intergenic 40.0%
! GGTGTTGTGGCAAAGTTAAT+GGG + chr4.4:24350773-24350792 MS.gene45403:CDS 40.0%
!! GGATGAGTAGTGTTGATTGT+AGG + chr4.4:24350849-24350868 MS.gene45403:CDS 40.0%
!! TGGTGTTGTGGCAAAGTTAA+TGG + chr4.4:24350772-24350791 MS.gene45403:CDS 40.0%
!! TGTGGCAAAGTTAATGGGTT+TGG + chr4.4:24350778-24350797 MS.gene45403:CDS 40.0%
AAATGGAGGAAGAGATGGCA+AGG + chr4.4:24350483-24350502 MS.gene45403:CDS 45.0%
AGTTGACATCACAAGCAGCA+AGG + chr4.4:24350379-24350398 MS.gene45403:CDS 45.0%
GAAGCTATTCCAGTGTCAGT+AGG + chr4.4:24350800-24350819 MS.gene45403:CDS 45.0%
TAAGAAGGAGGCCATTTGAG+AGG + chr4.4:24350882-24350901 MS.gene45403:CDS 45.0%
TTGGGAGCCAAGATGAAGAA+AGG + chr4.4:24350266-24350285 MS.gene45403:CDS 45.0%
! ACCGGTTTCTCTAGAAGCTA+TGG + chr4.4:24351015-24351034 MS.gene45403:CDS 45.0%
! GCCATAGCTTCTAGAGAAAC+CGG - chr4.4:24351019-24351038 None:intergenic 45.0%
! GCCATTTGAGAGGCATGATT+TGG + chr4.4:24350892-24350911 MS.gene45403:CDS 45.0%
! GTTTCTCTAGAAGCTATGGC+AGG + chr4.4:24351019-24351038 MS.gene45403:CDS 45.0%
! GTTTTCCACCAACAGTTGCT+GGG + chr4.4:24350726-24350745 MS.gene45403:CDS 45.0%
! TGCTGCTTGTGATGTCAACT+TGG - chr4.4:24350379-24350398 None:intergenic 45.0%
! TGTTTTCCACCAACAGTTGC+TGG + chr4.4:24350725-24350744 MS.gene45403:CDS 45.0%
! TTGGTGTAAACCTGGTGTTG+TGG + chr4.4:24350760-24350779 MS.gene45403:CDS 45.0%
! TTTTCCACCAACAGTTGCTG+GGG + chr4.4:24350727-24350746 MS.gene45403:CDS 45.0%
!! AACGTGGTTTGGTGTAAACC+TGG + chr4.4:24350752-24350771 MS.gene45403:CDS 45.0%
CTCCTTGGTTGCCAACTACT+AGG - chr4.4:24351049-24351068 None:intergenic 50.0%
GTCCTAGTAGTTGGCAACCA+AGG + chr4.4:24351044-24351063 MS.gene45403:CDS 50.0%
TGGCTCCCAAAGAGTTCTTG+AGG - chr4.4:24350256-24350275 None:intergenic 50.0%
! TCTCTAGAAGCTATGGCAGG+AGG + chr4.4:24351022-24351041 MS.gene45403:CDS 50.0%
AACAGTTGCTGGGGAGAACG+TGG + chr4.4:24350736-24350755 MS.gene45403:CDS 55.0%
ACGTTCTCCCCAGCAACTGT+TGG - chr4.4:24350737-24350756 None:intergenic 55.0%
TGGCAGGAGGTCCTAGTAGT+TGG + chr4.4:24351035-24351054 MS.gene45403:CDS 55.0%
TTCTCCCCAGCAACTGTTGG+TGG - chr4.4:24350734-24350753 None:intergenic 55.0%
TTGCTGGGGAGAACGTGGTT+TGG + chr4.4:24350741-24350760 MS.gene45403:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 24350239 24351078 24350239 ID=MS.gene45403
chr4.4 mRNA 24350239 24351078 24350239 ID=MS.gene45403.t1;Parent=MS.gene45403
chr4.4 exon 24350239 24351078 24350239 ID=MS.gene45403.t1.exon1;Parent=MS.gene45403.t1
chr4.4 CDS 24350239 24351078 24350239 ID=cds.MS.gene45403.t1;Parent=MS.gene45403.t1
Gene Sequence

>MS.gene45403

ATGCCATTCTTTCTCCTCAAGAACTCTTTGGGAGCCAAGATGAAGAAAGGCATAAGAACTTTCTGCAACACTGATGTGTCAACCTCAACTCTAAACCAACAAAATAGTACTATTGGCAATGGCATTGGCAACCATAGCCAAGTTGACATCACAAGCAGCAAGGTAAGCTCCCCTTTTTTGCAATGTGACAACTCAAATTCAAAGCAAAGCACACCAACATTAGAAGATCTTATACTACAATTAGAAATGGAGGAAGAGATGGCAAGGAAAGAAAAACTCAATGAATATAGTGGAATAAGGGGAAGGATGTCATGTGTTAACAACTCTGACATATTAAGATCTGCAAGGAATGCTTTGAATCAATATCCAAGGTTTTCACTTGATGGAAGAGATTCCATGTATAGGTCATCTTTTGGGACTATAGAAGGAAGAAGATCAGTTTGTAGTGAGATAAGTTTAGAACCAAAGCTTGATGAAAAGGTTATGTGTTTTCCACCAACAGTTGCTGGGGAGAACGTGGTTTGGTGTAAACCTGGTGTTGTGGCAAAGTTAATGGGTTTGGAAGCTATTCCAGTGTCAGTAGGTAGAAAAAGATCTGACAAAAAAGAGAGGATGAGTAGTGTTGATTGTAGGAGACAAAATATAAGAAGGAGGCCATTTGAGAGGCATGATTTGGAGAGAAAACTAGCAATGGACATGCAGGGTTATCGTGATCACATTGTTAGAAGAAGAAATAGGTCTGGTTTTTGCTCTAAAAATGGATATTGTATTATGAAACCGGTTTCTCTAGAAGCTATGGCAGGAGGTCCTAGTAGTTGGCAACCAAGGAGATATGTTTAG

Protein sequence

>MS.gene45403.t1

MPFFLLKNSLGAKMKKGIRTFCNTDVSTSTLNQQNSTIGNGIGNHSQVDITSSKVSSPFLQCDNSNSKQSTPTLEDLILQLEMEEEMARKEKLNEYSGIRGRMSCVNNSDILRSARNALNQYPRFSLDGRDSMYRSSFGTIEGRRSVCSEISLEPKLDEKVMCFPPTVAGENVVWCKPGVVAKLMGLEAIPVSVGRKRSDKKERMSSVDCRRQNIRRRPFERHDLERKLAMDMQGYRDHIVRRRNRSGFCSKNGYCIMKPVSLEAMAGGPSSWQPRRYV