Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58278.t1 | XP_003609436.1 | 97.6 | 82 | 2 | 0 | 1 | 82 | 279 | 360 | 1.30E-34 | 155.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58278.t1 | G7JFF7 | 97.6 | 82 | 2 | 0 | 1 | 82 | 279 | 360 | 9.3e-35 | 155.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene58278 | 0.800645 | 1.31E-48 | -1.69E-46 |
| MS.gene049159 | MS.gene58278 | 0.857773 | 1.30E-62 | -1.69E-46 |
| MS.gene049254 | MS.gene58278 | 0.81678 | 4.64E-52 | -1.69E-46 |
| MS.gene049343 | MS.gene58278 | 0.801887 | 7.28E-49 | -1.69E-46 |
| MS.gene049368 | MS.gene58278 | 0.806317 | 8.72E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene58278 | -0.808488 | 3.02E-50 | -1.69E-46 |
| MS.gene049452 | MS.gene58278 | 0.867904 | 9.70E-66 | -1.69E-46 |
| MS.gene049499 | MS.gene58278 | 0.800765 | 1.24E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene58278 | 0.847929 | 8.47E-60 | -1.69E-46 |
| MS.gene050605 | MS.gene58278 | 0.807968 | 3.90E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene58278 | 0.803655 | 3.14E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene58278 | 0.811424 | 7.05E-51 | -1.69E-46 |
| MS.gene050737 | MS.gene58278 | 0.800515 | 1.39E-48 | -1.69E-46 |
| MS.gene050759 | MS.gene58278 | 0.812947 | 3.28E-51 | -1.69E-46 |
| MS.gene050905 | MS.gene58278 | -0.801185 | 1.01E-48 | -1.69E-46 |
| MS.gene050941 | MS.gene58278 | 0.802336 | 5.89E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene58278 | 0.804753 | 1.86E-49 | -1.69E-46 |
| MS.gene051181 | MS.gene58278 | 0.811944 | 5.43E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene58278 | 0.809111 | 2.22E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene58278 | 0.803598 | 3.23E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene58278 | 0.808933 | 2.43E-50 | -1.69E-46 |
| MS.gene051452 | MS.gene58278 | 0.808565 | 2.91E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene58278 | 0.805634 | 1.21E-49 | -1.69E-46 |
| MS.gene052518 | MS.gene58278 | 0.80153 | 8.62E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene58278 | 0.847046 | 1.48E-59 | -1.69E-46 |
| MS.gene052773 | MS.gene58278 | 0.810839 | 9.44E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene58278 | 0.830325 | 3.13E-55 | -1.69E-46 |
| MS.gene052954 | MS.gene58278 | 0.822067 | 2.89E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene58278 | 0.826885 | 2.12E-54 | -1.69E-46 |
| MS.gene053008 | MS.gene58278 | 0.815306 | 9.89E-52 | -1.69E-46 |
| MS.gene05308 | MS.gene58278 | 0.81537 | 9.57E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene58278 | 0.810245 | 1.27E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene58278 | 0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene05350 | MS.gene58278 | 0.805059 | 1.60E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene58278 | 0.849706 | 2.72E-60 | -1.69E-46 |
| MS.gene05415 | MS.gene58278 | 0.831117 | 2.00E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene58278 | 0.823729 | 1.18E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene58278 | 0.806781 | 6.96E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene58278 | 0.820623 | 6.22E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene58278 | 0.816084 | 6.63E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene58278 | 0.854999 | 8.47E-62 | -1.69E-46 |
| MS.gene054775 | MS.gene58278 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene054994 | MS.gene58278 | 0.808788 | 2.61E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene58278 | 0.809771 | 1.60E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene58278 | 0.819265 | 1.27E-52 | -1.69E-46 |
| MS.gene055222 | MS.gene58278 | 0.837765 | 4.29E-57 | -1.69E-46 |
| MS.gene055228 | MS.gene58278 | 0.803507 | 3.37E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene58278 | 0.801022 | 1.10E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene58278 | 0.820489 | 6.68E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene58278 | 0.827365 | 1.63E-54 | -1.69E-46 |
| MS.gene055841 | MS.gene58278 | 0.81403 | 1.90E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene58278 | 0.805386 | 1.37E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene58278 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene58278 | 0.821816 | 3.30E-53 | -1.69E-46 |
| MS.gene05690 | MS.gene58278 | 0.82025 | 7.58E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene58278 | 0.870659 | 1.23E-66 | -1.69E-46 |
| MS.gene057598 | MS.gene58278 | 0.812915 | 3.33E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene58278 | 0.814925 | 1.20E-51 | -1.69E-46 |
| MS.gene057855 | MS.gene58278 | 0.831308 | 1.80E-55 | -1.69E-46 |
| MS.gene058008 | MS.gene58278 | 0.814919 | 1.21E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene58278 | 0.832049 | 1.18E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene58278 | 0.819317 | 1.24E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene58278 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene58278 | 0.800662 | 1.30E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene58278 | 0.80758 | 4.72E-50 | -1.69E-46 |
| MS.gene058427 | MS.gene58278 | 0.817206 | 3.72E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene58278 | 0.811506 | 6.77E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene58278 | 0.830123 | 3.51E-55 | -1.69E-46 |
| MS.gene058783 | MS.gene58278 | 0.856437 | 3.22E-62 | -1.69E-46 |
| MS.gene058979 | MS.gene58278 | 0.846386 | 2.24E-59 | -1.69E-46 |
| MS.gene059283 | MS.gene58278 | 0.81081 | 9.58E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene58278 | 0.843826 | 1.10E-58 | -1.69E-46 |
| MS.gene060011 | MS.gene58278 | 0.830634 | 2.63E-55 | -1.69E-46 |
| MS.gene060244 | MS.gene58278 | 0.810498 | 1.12E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene58278 | 0.818194 | 2.23E-52 | -1.69E-46 |
| MS.gene060310 | MS.gene58278 | 0.82956 | 4.81E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene58278 | 0.802428 | 5.64E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene58278 | 0.804486 | 2.11E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene58278 | 0.801922 | 7.16E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene58278 | 0.861186 | 1.22E-63 | -1.69E-46 |
| MS.gene060937 | MS.gene58278 | 0.843362 | 1.47E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGGGTGAAATATTAGTT+AGG | 0.342748 | 8.3:-10660194 | MS.gene58278:CDS |
| ATTTGTTGAGTTGCAGAGAA+TGG | 0.376884 | 8.3:-10660301 | MS.gene58278:CDS |
| AGCGAGATTTGCGGTGCAAT+TGG | 0.379941 | 8.3:-10660256 | MS.gene58278:CDS |
| TTGCGGTGCAATTGGACGTT+GGG | 0.391803 | 8.3:-10660248 | MS.gene58278:CDS |
| GTGCAATTGGACGTTGGGGA+AGG | 0.421848 | 8.3:-10660243 | MS.gene58278:CDS |
| TAAGGGTGAAATATTAGTTA+GGG | 0.484187 | 8.3:-10660193 | MS.gene58278:CDS |
| AGCTGTTGAAGGCATGTTGA+AGG | 0.520705 | 8.3:-10660340 | MS.gene58278:CDS |
| GGAAGGAAGGAAGCTGTTGA+AGG | 0.528810 | 8.3:-10660351 | None:intergenic |
| TGCAATTGGACGTTGGGGAA+GGG | 0.539508 | 8.3:-10660242 | MS.gene58278:CDS |
| GAGCTGTGTAGCGAGATTTG+CGG | 0.572894 | 8.3:-10660265 | MS.gene58278:CDS |
| GGTTAAGACAAAGAGAGAAG+AGG | 0.576455 | 8.3:-10660221 | MS.gene58278:CDS |
| CCACAAAACTTCAGCAACAA+TGG | 0.581435 | 8.3:+10660134 | None:intergenic |
| TGCGGTGCAATTGGACGTTG+GGG | 0.585281 | 8.3:-10660247 | MS.gene58278:CDS |
| TTTGCGGTGCAATTGGACGT+TGG | 0.585765 | 8.3:-10660249 | MS.gene58278:CDS |
| GTTGAAGGCATGTTGAAGGA+AGG | 0.684693 | 8.3:-10660336 | MS.gene58278:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAGGGTGAAATATTAGTTA+GGG | - | chr8.3:10660272-10660291 | MS.gene58278:CDS | 25.0% |
| ! | TTAAGGGTGAAATATTAGTT+AGG | - | chr8.3:10660271-10660290 | MS.gene58278:CDS | 25.0% |
| ! | AAGAGAGAAGAGGAGTTTTA+AGG | - | chr8.3:10660254-10660273 | MS.gene58278:CDS | 35.0% |
| ! | ATTTGTTGAGTTGCAGAGAA+TGG | - | chr8.3:10660164-10660183 | MS.gene58278:CDS | 35.0% |
| !! | AGAGAGAAGAGGAGTTTTAA+GGG | - | chr8.3:10660255-10660274 | MS.gene58278:CDS | 35.0% |
| !! | ATTGTTGCTGAAGTTTTGTG+GGG | - | chr8.3:10660333-10660352 | MS.gene58278:CDS | 35.0% |
| !! | CATTGTTGCTGAAGTTTTGT+GGG | - | chr8.3:10660332-10660351 | MS.gene58278:CDS | 35.0% |
| CCACAAAACTTCAGCAACAA+TGG | + | chr8.3:10660334-10660353 | None:intergenic | 40.0% | |
| GGTTAAGACAAAGAGAGAAG+AGG | - | chr8.3:10660244-10660263 | MS.gene58278:CDS | 40.0% | |
| ! | CCATTGTTGCTGAAGTTTTG+TGG | - | chr8.3:10660331-10660350 | MS.gene58278:CDS | 40.0% |
| ! | AGCTGTTGAAGGCATGTTGA+AGG | - | chr8.3:10660125-10660144 | MS.gene58278:CDS | 45.0% |
| ! | GTTGAAGGCATGTTGAAGGA+AGG | - | chr8.3:10660129-10660148 | MS.gene58278:CDS | 45.0% |
| ! | TTTGTGGGGTACTTCTCCAT+AGG | - | chr8.3:10660347-10660366 | MS.gene58278:CDS | 45.0% |
| AGCGAGATTTGCGGTGCAAT+TGG | - | chr8.3:10660209-10660228 | MS.gene58278:CDS | 50.0% | |
| GAGCTGTGTAGCGAGATTTG+CGG | - | chr8.3:10660200-10660219 | MS.gene58278:CDS | 50.0% | |
| TGCAATTGGACGTTGGGGAA+GGG | - | chr8.3:10660223-10660242 | MS.gene58278:CDS | 50.0% | |
| TTGCGGTGCAATTGGACGTT+GGG | - | chr8.3:10660217-10660236 | MS.gene58278:CDS | 50.0% | |
| TTTGCGGTGCAATTGGACGT+TGG | - | chr8.3:10660216-10660235 | MS.gene58278:CDS | 50.0% | |
| GTGCAATTGGACGTTGGGGA+AGG | - | chr8.3:10660222-10660241 | MS.gene58278:CDS | 55.0% | |
| TGCGGTGCAATTGGACGTTG+GGG | - | chr8.3:10660218-10660237 | MS.gene58278:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10660119 | 10660368 | 10660119 | ID=MS.gene58278 |
| chr8.3 | mRNA | 10660119 | 10660368 | 10660119 | ID=MS.gene58278.t1;Parent=MS.gene58278 |
| chr8.3 | exon | 10660119 | 10660368 | 10660119 | ID=MS.gene58278.t1.exon1;Parent=MS.gene58278.t1 |
| chr8.3 | CDS | 10660119 | 10660368 | 10660119 | ID=cds.MS.gene58278.t1;Parent=MS.gene58278.t1 |
| Gene Sequence |
| Protein sequence |
