AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62835


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62835.t1 MTR_4g102220 96.889 225 7 0 1 225 1 225 9.53e-160 441
MS.gene62835.t1 MTR_5g011800 59.574 235 79 6 1 225 1 229 2.59e-86 255
MS.gene62835.t1 MTR_5g011820 57.021 235 85 7 1 225 4 232 2.77e-81 243
MS.gene62835.t1 MTR_4g024370 52.521 238 92 8 1 224 1 231 3.02e-72 219
MS.gene62835.t1 MTR_4g024370 52.521 238 92 8 1 224 1 231 3.02e-72 219
MS.gene62835.t1 MTR_4g023950 32.143 224 128 8 5 224 4 207 3.96e-23 93.2
MS.gene62835.t1 MTR_1g076800 31.556 225 132 7 5 224 4 211 9.67e-23 92.4
MS.gene62835.t1 MTR_6g060390 30.837 227 132 10 5 224 4 212 1.11e-22 92.0
MS.gene62835.t1 MTR_6g060440 28.444 225 138 7 5 224 4 210 2.21e-22 91.3
MS.gene62835.t1 MTR_7g117380 31.466 232 134 11 5 224 3 221 4.45e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62835.t1 AT2G45360 54.867 226 90 4 1 225 1 215 6.92e-72 218
MS.gene62835.t1 AT3G60780 51.316 228 98 5 1 225 1 218 4.50e-69 211
MS.gene62835.t1 AT1G62840 49.569 232 102 5 1 225 1 224 1.00e-60 190
MS.gene62835.t1 AT1G12320 47.788 226 99 5 1 223 1 210 1.50e-51 166
MS.gene62835.t1 AT5G62280 29.487 234 138 10 5 224 4 224 4.10e-16 75.1

Find 44 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGATTGTGAGGGAATTA+AGG 0.232972 8.1:+21385975 MS.gene62835:CDS
TAAGGTCACGTCGTGTTGTT+AGG 0.340628 8.1:+21386121 MS.gene62835:CDS
TAGCACATGTTTCAGCTGTT+GGG 0.366973 8.1:+21386178 MS.gene62835:CDS
GGTGGCTAAGTTAAGTGTTA+AGG 0.386848 8.1:+21386017 MS.gene62835:CDS
TTGTTGAGGAAGGAGGATTA+AGG 0.425189 8.1:+21386103 MS.gene62835:CDS
AGGTCGGTGTTTCTTCCTGT+TGG 0.430254 8.1:+21386141 MS.gene62835:CDS
TGCGTTCCATCCTGCTGCCA+TGG 0.440434 8.1:-21385725 None:intergenic
AAGCATGTTGATCAACGGTC+AGG 0.453278 8.1:+21386252 MS.gene62835:CDS
ATGTCAAGTCCCTTCCCAAC+AGG 0.460059 8.1:-21386156 None:intergenic
GGGAATTAAGGATTTGATGA+AGG 0.460412 8.1:+21385987 MS.gene62835:CDS
GGTCGGTGTTTCTTCCTGTT+GGG 0.463683 8.1:+21386142 MS.gene62835:CDS
GTGGCTAAGTTAAGTGTTAA+GGG 0.463703 8.1:+21386018 MS.gene62835:CDS
GTGTTTCTTCCTGTTGGGAA+GGG 0.468303 8.1:+21386147 MS.gene62835:CDS
ATAGCACATGTTTCAGCTGT+TGG 0.469433 8.1:+21386177 MS.gene62835:CDS
AAGAGAAAATGAAACTTGTC+TGG 0.470997 8.1:+21385420 None:intergenic
GGTGTTTCTTCCTGTTGGGA+AGG 0.479058 8.1:+21386146 MS.gene62835:CDS
CATTGAAGAGATAGATTTCG+TGG 0.482218 8.1:+21385951 MS.gene62835:CDS
CTGATTTCAGCCATGGCAGC+AGG 0.486556 8.1:+21385715 MS.gene62835:CDS
GATATTATTGTTGAGGAAGG+AGG 0.500593 8.1:+21386096 MS.gene62835:CDS
TGAAATCAGCTCCGCCATGC+CGG 0.504005 8.1:-21385701 None:intergenic
ACATCAACAGAAATCATTGT+AGG 0.506787 8.1:+21385901 MS.gene62835:CDS
GGTGTAATCGAAACAAGCTT+AGG 0.508623 8.1:+21385778 MS.gene62835:CDS
AGCACATGTTTCAGCTGTTG+GGG 0.510606 8.1:+21386179 MS.gene62835:CDS
GAGGATATTATTGTTGAGGA+AGG 0.517535 8.1:+21386093 MS.gene62835:CDS
GGCGGAGCTGATTTCAGCCA+TGG 0.524952 8.1:+21385708 MS.gene62835:CDS
GGAAAAGATGGTCATGGTCG+TGG 0.531446 8.1:+21386210 MS.gene62835:CDS
GTCACGTCGTGTTGTTAGGT+CGG 0.536069 8.1:+21386125 MS.gene62835:CDS
AGCATGTTGATCAACGGTCA+GGG 0.549561 8.1:+21386253 MS.gene62835:CDS
ATGGGAGGATATTATTGTTG+AGG 0.550906 8.1:+21386089 MS.gene62835:CDS
TTTCGTGGTGGTAGATTGTG+AGG 0.551539 8.1:+21385966 MS.gene62835:CDS
GTCGTGGTGGTAGTAAGAGA+TGG 0.579545 8.1:+21386226 MS.gene62835:CDS
TTTCAGCCATGGCAGCAGGA+TGG 0.596118 8.1:+21385719 MS.gene62835:CDS
ATAGTGGAAACATGGTCAGA+AGG 0.600619 8.1:+21385754 MS.gene62835:CDS
TTCGTGGTGGTAGATTGTGA+GGG 0.604870 8.1:+21385967 MS.gene62835:CDS
GGGAGGTGCATGTTATTAGA+CGG 0.626486 8.1:+21386274 MS.gene62835:CDS
GCATGTTGATCAACGGTCAG+GGG 0.633202 8.1:+21386254 MS.gene62835:CDS
GGATTAAGCATGTTGATCAA+CGG 0.643256 8.1:+21386247 MS.gene62835:CDS
TGTTGATCAACGGTCAGGGG+AGG 0.647427 8.1:+21386257 MS.gene62835:CDS
GTGGAAACATGGTCAGAAGG+AGG 0.667051 8.1:+21385757 MS.gene62835:CDS
AAAGATGGTCATGGTCGTGG+TGG 0.675881 8.1:+21386213 MS.gene62835:CDS
AATTTGGGAAAAGATGGTCA+TGG 0.694637 8.1:+21386204 MS.gene62835:CDS
GCACATGTTTCAGCTGTTGG+GGG 0.717069 8.1:+21386180 MS.gene62835:CDS
TGAAGAGATAGATTTCGTGG+TGG 0.721855 8.1:+21385954 MS.gene62835:CDS
TTTACGTGGATCCGGCATGG+CGG 0.735740 8.1:+21385690 MS.gene62835:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTATTCAAGATTAATA+TGG - chr8.1:21385547-21385566 None:intergenic 10.0%
!! ATTATTATTAACAACAGTAA+CGG - chr8.1:21385645-21385664 None:intergenic 15.0%
!!! TATGTAAGAATGTTAATTTT+AGG + chr8.1:21386052-21386071 MS.gene62835:CDS 15.0%
!! ACTAGAGTACTCTAAAAAAA+TGG + chr8.1:21385867-21385886 MS.gene62835:CDS 25.0%
!! CTAGAGTACTCTAAAAAAAT+GGG + chr8.1:21385868-21385887 MS.gene62835:CDS 25.0%
!!! AAGAATGTTAATTTTAGGAG+TGG + chr8.1:21386057-21386076 MS.gene62835:CDS 25.0%
!!! AGAATGTTAATTTTAGGAGT+GGG + chr8.1:21386058-21386077 MS.gene62835:CDS 25.0%
ACATCAACAGAAATCATTGT+AGG + chr8.1:21385901-21385920 MS.gene62835:CDS 30.0%
TCTAAAAAAATGGGTGAACA+AGG + chr8.1:21385877-21385896 MS.gene62835:CDS 30.0%
! ATAGCAAAAAAGCACACAAA+TGG + chr8.1:21385808-21385827 MS.gene62835:CDS 30.0%
! TAGCAAAAAAGCACACAAAT+GGG + chr8.1:21385809-21385828 MS.gene62835:CDS 30.0%
! TAGGAGTGGGAATTTTAAAT+GGG + chr8.1:21386071-21386090 MS.gene62835:CDS 30.0%
! TTAGGAGTGGGAATTTTAAA+TGG + chr8.1:21386070-21386089 MS.gene62835:CDS 30.0%
!! CAAAAATGATAGTGGAAACA+TGG + chr8.1:21385746-21385765 MS.gene62835:CDS 30.0%
!!! TCTAGTTTTGATATCTCGTT+TGG - chr8.1:21385853-21385872 None:intergenic 30.0%
AATTTGGGAAAAGATGGTCA+TGG + chr8.1:21386204-21386223 MS.gene62835:CDS 35.0%
ATGGAACGCAAAAATGATAG+TGG + chr8.1:21385738-21385757 MS.gene62835:CDS 35.0%
ATGGGAGGATATTATTGTTG+AGG + chr8.1:21386089-21386108 MS.gene62835:CDS 35.0%
CATGTATGTGTGTAAGGATT+TGG - chr8.1:21385499-21385518 None:intergenic 35.0%
CATTGAAGAGATAGATTTCG+TGG + chr8.1:21385951-21385970 MS.gene62835:CDS 35.0%
GAGGATATTATTGTTGAGGA+AGG + chr8.1:21386093-21386112 MS.gene62835:CDS 35.0%
GATATTATTGTTGAGGAAGG+AGG + chr8.1:21386096-21386115 MS.gene62835:CDS 35.0%
GGATTAAGCATGTTGATCAA+CGG + chr8.1:21386247-21386266 MS.gene62835:CDS 35.0%
GGGAATTAAGGATTTGATGA+AGG + chr8.1:21385987-21386006 MS.gene62835:CDS 35.0%
GTGGCTAAGTTAAGTGTTAA+GGG + chr8.1:21386018-21386037 MS.gene62835:CDS 35.0%
TTCATGCATGTATGTGTGTA+AGG - chr8.1:21385505-21385524 None:intergenic 35.0%
!! GGATTTGATGAAGGTTTTGA+AGG + chr8.1:21385996-21386015 MS.gene62835:CDS 35.0%
!!! ATGCTTTTGATGCTGTTTCA+GGG - chr8.1:21385448-21385467 None:intergenic 35.0%
!!! TATGCTTTTGATGCTGTTTC+AGG - chr8.1:21385449-21385468 None:intergenic 35.0%
!!! TTTGATGAAGGTTTTGAAGG+TGG + chr8.1:21385999-21386018 MS.gene62835:CDS 35.0%
ATAGCACATGTTTCAGCTGT+TGG + chr8.1:21386177-21386196 MS.gene62835:CDS 40.0%
GGTAGATTGTGAGGGAATTA+AGG + chr8.1:21385975-21385994 MS.gene62835:CDS 40.0%
GGTGGCTAAGTTAAGTGTTA+AGG + chr8.1:21386017-21386036 MS.gene62835:CDS 40.0%
GGTGTAATCGAAACAAGCTT+AGG + chr8.1:21385778-21385797 MS.gene62835:CDS 40.0%
TAGCACATGTTTCAGCTGTT+GGG + chr8.1:21386178-21386197 MS.gene62835:CDS 40.0%
TGAAGAGATAGATTTCGTGG+TGG + chr8.1:21385954-21385973 MS.gene62835:CDS 40.0%
TTGTTGAGGAAGGAGGATTA+AGG + chr8.1:21386103-21386122 MS.gene62835:CDS 40.0%
! ATAGTGGAAACATGGTCAGA+AGG + chr8.1:21385754-21385773 MS.gene62835:CDS 40.0%
! GAGTGGGAATTTTAAATGGG+AGG + chr8.1:21386074-21386093 MS.gene62835:CDS 40.0%
! GTAAAACCTTGCACTGATGT+AGG - chr8.1:21385675-21385694 None:intergenic 40.0%
!!! TGCTTTTGATGCTGTTTCAG+GGG - chr8.1:21385447-21385466 None:intergenic 40.0%
AAGCATGTTGATCAACGGTC+AGG + chr8.1:21386252-21386271 MS.gene62835:CDS 45.0%
AGCACATGTTTCAGCTGTTG+GGG + chr8.1:21386179-21386198 MS.gene62835:CDS 45.0%
AGCATGTTGATCAACGGTCA+GGG + chr8.1:21386253-21386272 MS.gene62835:CDS 45.0%
GGGGGTAATTTGGGAAAAGA+TGG + chr8.1:21386198-21386217 MS.gene62835:CDS 45.0%
GTGTTTCTTCCTGTTGGGAA+GGG + chr8.1:21386147-21386166 MS.gene62835:CDS 45.0%
TCAGCTGTTGGGGGTAATTT+GGG + chr8.1:21386189-21386208 MS.gene62835:CDS 45.0%
TTCAGCTGTTGGGGGTAATT+TGG + chr8.1:21386188-21386207 MS.gene62835:CDS 45.0%
TTCGTGGTGGTAGATTGTGA+GGG + chr8.1:21385967-21385986 MS.gene62835:CDS 45.0%
TTCTGACCTACATCAGTGCA+AGG + chr8.1:21385666-21385685 MS.gene62835:intron 45.0%
TTTCGTGGTGGTAGATTGTG+AGG + chr8.1:21385966-21385985 MS.gene62835:CDS 45.0%
! CATCAGTGCAAGGTTTTACG+TGG + chr8.1:21385676-21385695 MS.gene62835:intron 45.0%
! GGGAGGTGCATGTTATTAGA+CGG + chr8.1:21386274-21386293 MS.gene62835:CDS 45.0%
! TAAGGTCACGTCGTGTTGTT+AGG + chr8.1:21386121-21386140 MS.gene62835:CDS 45.0%
! TGCAAGGTTTTACGTGGATC+CGG + chr8.1:21385682-21385701 MS.gene62835:CDS 45.0%
AAAGATGGTCATGGTCGTGG+TGG + chr8.1:21386213-21386232 MS.gene62835:CDS 50.0%
ATGTCAAGTCCCTTCCCAAC+AGG - chr8.1:21386159-21386178 None:intergenic 50.0%
GCACATGTTTCAGCTGTTGG+GGG + chr8.1:21386180-21386199 MS.gene62835:CDS 50.0%
GCATGTTGATCAACGGTCAG+GGG + chr8.1:21386254-21386273 MS.gene62835:CDS 50.0%
GGAAAAGATGGTCATGGTCG+TGG + chr8.1:21386210-21386229 MS.gene62835:CDS 50.0%
GGTGTTTCTTCCTGTTGGGA+AGG + chr8.1:21386146-21386165 MS.gene62835:CDS 50.0%
GTCGTGGTGGTAGTAAGAGA+TGG + chr8.1:21386226-21386245 MS.gene62835:CDS 50.0%
GTGGAAACATGGTCAGAAGG+AGG + chr8.1:21385757-21385776 MS.gene62835:CDS 50.0%
! AGGTCGGTGTTTCTTCCTGT+TGG + chr8.1:21386141-21386160 MS.gene62835:CDS 50.0%
! GGTCGGTGTTTCTTCCTGTT+GGG + chr8.1:21386142-21386161 MS.gene62835:CDS 50.0%
! GTCACGTCGTGTTGTTAGGT+CGG + chr8.1:21386125-21386144 MS.gene62835:CDS 50.0%
CTGATTTCAGCCATGGCAGC+AGG + chr8.1:21385715-21385734 MS.gene62835:CDS 55.0%
TGAAATCAGCTCCGCCATGC+CGG - chr8.1:21385704-21385723 None:intergenic 55.0%
TGTTGATCAACGGTCAGGGG+AGG + chr8.1:21386257-21386276 MS.gene62835:CDS 55.0%
TTTACGTGGATCCGGCATGG+CGG + chr8.1:21385690-21385709 MS.gene62835:CDS 55.0%
TTTCAGCCATGGCAGCAGGA+TGG + chr8.1:21385719-21385738 MS.gene62835:CDS 55.0%
! GGTTTTACGTGGATCCGGCA+TGG + chr8.1:21385687-21385706 MS.gene62835:CDS 55.0%
TGCGTTCCATCCTGCTGCCA+TGG - chr8.1:21385728-21385747 None:intergenic 60.0%
! GGCGGAGCTGATTTCAGCCA+TGG + chr8.1:21385708-21385727 MS.gene62835:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 21385428 21386299 21385428 ID=MS.gene62835
chr8.1 mRNA 21385428 21386299 21385428 ID=MS.gene62835.t1;Parent=MS.gene62835
chr8.1 exon 21385428 21385487 21385428 ID=MS.gene62835.t1.exon1;Parent=MS.gene62835.t1
chr8.1 CDS 21385428 21385487 21385428 ID=cds.MS.gene62835.t1;Parent=MS.gene62835.t1
chr8.1 exon 21385682 21386299 21385682 ID=MS.gene62835.t1.exon2;Parent=MS.gene62835.t1
chr8.1 CDS 21385682 21386299 21385682 ID=cds.MS.gene62835.t1;Parent=MS.gene62835.t1
Gene Sequence

>MS.gene62835

ATGAAACTTGTCTGGTCCCCTGAAACAGCATCAAAAGCATACATCGACACAGTTCAATCTGTAAGCAACCAAATCCTTACACACATACATGCATGAATTAATCTTCTTTTTCATTTCCATATTAATCTTGAATAAATAAAATTTTCTCAGCTTAGCGATAAGAGTTTGACTTTACAAAACATAGTCAAAATCTCATCGCATGTTGTAAAAATAACCGTTACTGTTGTTAATAATAATTTTCTGACCTACATCAGTGCAAGGTTTTACGTGGATCCGGCATGGCGGAGCTGATTTCAGCCATGGCAGCAGGATGGAACGCAAAAATGATAGTGGAAACATGGTCAGAAGGAGGTGTAATCGAAACAAGCTTAGGTTTATCAATAGCAAAAAAGCACACAAATGGGAGACACGTATGCATAGTTCCAAACGAGATATCAAAACTAGAGTACTCTAAAAAAATGGGTGAACAAGGAACATCAACAGAAATCATTGTAGGAGAAGCAGAAGAAGTTATGAAAGATTTCATTGAAGAGATAGATTTCGTGGTGGTAGATTGTGAGGGAATTAAGGATTTGATGAAGGTTTTGAAGGTGGCTAAGTTAAGTGTTAAGGGTGCAGTTTTGATATGTAAGAATGTTAATTTTAGGAGTGGGAATTTTAAATGGGAGGATATTATTGTTGAGGAAGGAGGATTAAGGTCACGTCGTGTTGTTAGGTCGGTGTTTCTTCCTGTTGGGAAGGGACTTGACATAGCACATGTTTCAGCTGTTGGGGGTAATTTGGGAAAAGATGGTCATGGTCGTGGTGGTAGTAAGAGATGGATTAAGCATGTTGATCAACGGTCAGGGGAGGTGCATGTTATTAGACGGTGA

Protein sequence

>MS.gene62835.t1

MKLVWSPETASKAYIDTVQSCKVLRGSGMAELISAMAAGWNAKMIVETWSEGGVIETSLGLSIAKKHTNGRHVCIVPNEISKLEYSKKMGEQGTSTEIIVGEAEEVMKDFIEEIDFVVVDCEGIKDLMKVLKVAKLSVKGAVLICKNVNFRSGNFKWEDIIVEEGGLRSRRVVRSVFLPVGKGLDIAHVSAVGGNLGKDGHGRGGSKRWIKHVDQRSGEVHVIRR