AlfalfaGEDB | MS.gene64414

M. sativa cultivar XinJiangDaYe / MS.gene64414

KEGG: -
GO: GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009523 GO:0009987 GO:0015979 GO:0016020 GO:0032991 GO:0044237 GO:0098796 GO:0110165
PFAM: PF06596.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64414.t1	XP_013463512.1	95	120	5	1	1	119	1	120	2.40E-42	181.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64414.t1	A0A072V6T7	95.0	120	5	1	1	119	1	120	1.8e-42	181.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049427	MS.gene64414	0.836842	7.38E-57	-1.69E-46
MS.gene049491	MS.gene64414	0.856176	3.84E-62	-1.69E-46
MS.gene049614	MS.gene64414	0.830639	2.62E-55	-1.69E-46
MS.gene049661	MS.gene64414	0.826351	2.85E-54	-1.69E-46
MS.gene049662	MS.gene64414	0.818566	1.83E-52	-1.69E-46
MS.gene049760	MS.gene64414	0.809473	1.86E-50	-1.69E-46
MS.gene049805	MS.gene64414	0.801944	7.09E-49	-1.69E-46
MS.gene050076	MS.gene64414	0.862157	6.18E-64	-1.69E-46
MS.gene050432	MS.gene64414	0.866182	3.44E-65	-1.69E-46
MS.gene05053	MS.gene64414	0.805926	1.05E-49	-1.69E-46
MS.gene050767	MS.gene64414	0.900018	1.11E-77	-1.69E-46
MS.gene050770	MS.gene64414	0.901091	3.80E-78	-1.69E-46
MS.gene05101	MS.gene64414	0.845717	3.41E-59	-1.69E-46
MS.gene051228	MS.gene64414	0.847862	8.84E-60	-1.69E-46
MS.gene051471	MS.gene64414	0.800025	1.75E-48	-1.69E-46
MS.gene051692	MS.gene64414	0.810402	1.17E-50	-1.69E-46
MS.gene051810	MS.gene64414	0.80475	1.86E-49	-1.69E-46
MS.gene052078	MS.gene64414	0.808363	3.21E-50	-1.69E-46
MS.gene052271	MS.gene64414	0.867966	9.27E-66	-1.69E-46
MS.gene052290	MS.gene64414	0.863432	2.50E-64	-1.69E-46
MS.gene052293	MS.gene64414	0.832944	7.08E-56	-1.69E-46
MS.gene052310	MS.gene64414	0.83152	1.59E-55	-1.69E-46
MS.gene052326	MS.gene64414	0.845506	3.89E-59	-1.69E-46
MS.gene052379	MS.gene64414	0.846801	1.73E-59	-1.69E-46
MS.gene052484	MS.gene64414	0.820444	6.84E-53	-1.69E-46
MS.gene053009	MS.gene64414	0.83972	1.34E-57	-1.69E-46
MS.gene053103	MS.gene64414	0.834545	2.82E-56	-1.69E-46
MS.gene053104	MS.gene64414	0.845289	4.46E-59	-1.69E-46
MS.gene053209	MS.gene64414	0.824967	6.06E-54	-1.69E-46
MS.gene053322	MS.gene64414	0.869798	2.36E-66	-1.69E-46
MS.gene053384	MS.gene64414	0.822937	1.81E-53	-1.69E-46
MS.gene053813	MS.gene64414	0.833934	4.01E-56	-1.69E-46
MS.gene054376	MS.gene64414	0.903486	3.30E-79	-1.69E-46
MS.gene054543	MS.gene64414	0.817746	2.81E-52	-1.69E-46
MS.gene054642	MS.gene64414	0.809031	2.31E-50	-1.69E-46
MS.gene054802	MS.gene64414	0.844509	7.24E-59	-1.69E-46
MS.gene054803	MS.gene64414	0.84677	1.76E-59	-1.69E-46
MS.gene054804	MS.gene64414	0.848083	7.68E-60	-1.69E-46
MS.gene055254	MS.gene64414	0.867698	1.13E-65	-1.69E-46
MS.gene055277	MS.gene64414	0.852844	3.53E-61	-1.69E-46
MS.gene055397	MS.gene64414	0.875943	2.07E-68	-1.69E-46
MS.gene055497	MS.gene64414	-0.807743	4.35E-50	-1.69E-46
MS.gene055499	MS.gene64414	0.869351	3.31E-66	-1.69E-46
MS.gene055636	MS.gene64414	0.835691	1.45E-56	-1.69E-46
MS.gene055638	MS.gene64414	0.801668	8.08E-49	-1.69E-46
MS.gene055647	MS.gene64414	0.865046	7.84E-65	-1.69E-46
MS.gene055722	MS.gene64414	0.807624	4.62E-50	-1.69E-46
MS.gene055896	MS.gene64414	0.822396	2.42E-53	-1.69E-46
MS.gene05592	MS.gene64414	0.872344	3.42E-67	-1.69E-46
MS.gene056374	MS.gene64414	0.832324	1.01E-55	-1.69E-46
MS.gene056410	MS.gene64414	0.831346	1.76E-55	-1.69E-46
MS.gene056609	MS.gene64414	0.84744	1.16E-59	-1.69E-46
MS.gene056897	MS.gene64414	0.80203	6.81E-49	-1.69E-46
MS.gene057011	MS.gene64414	0.829076	6.30E-55	-1.69E-46
MS.gene05702	MS.gene64414	0.80553	1.28E-49	-1.69E-46
MS.gene057123	MS.gene64414	0.828987	6.62E-55	-1.69E-46
MS.gene057129	MS.gene64414	0.874586	6.02E-68	-1.69E-46
MS.gene057146	MS.gene64414	0.807565	4.75E-50	-1.69E-46
MS.gene057285	MS.gene64414	0.800053	1.73E-48	-1.69E-46
MS.gene057295	MS.gene64414	0.833014	6.80E-56	-1.69E-46
MS.gene057331	MS.gene64414	0.818705	1.70E-52	-1.69E-46
MS.gene05735	MS.gene64414	0.813686	2.26E-51	-1.69E-46
MS.gene057416	MS.gene64414	0.828024	1.13E-54	-1.69E-46
MS.gene058019	MS.gene64414	0.804778	1.83E-49	-1.69E-46
MS.gene058293	MS.gene64414	0.826064	3.33E-54	-1.69E-46
MS.gene058660	MS.gene64414	0.843325	1.50E-58	-1.69E-46
MS.gene058903	MS.gene64414	0.820096	8.21E-53	-1.69E-46
MS.gene059029	MS.gene64414	0.815877	7.38E-52	-1.69E-46
MS.gene059326	MS.gene64414	0.829437	5.15E-55	-1.69E-46
MS.gene059536	MS.gene64414	0.84551	3.88E-59	-1.69E-46
MS.gene059597	MS.gene64414	0.810364	1.20E-50	-1.69E-46
MS.gene059676	MS.gene64414	0.8245	7.80E-54	-1.69E-46
MS.gene059688	MS.gene64414	0.820638	6.17E-53	-1.69E-46
MS.gene059692	MS.gene64414	0.828407	9.15E-55	-1.69E-46
MS.gene059695	MS.gene64414	0.824191	9.23E-54	-1.69E-46
MS.gene059711	MS.gene64414	0.845698	3.45E-59	-1.69E-46
MS.gene059894	MS.gene64414	0.811007	8.68E-51	-1.69E-46
MS.gene059960	MS.gene64414	0.867326	1.49E-65	-1.69E-46
MS.gene059974	MS.gene64414	0.823054	1.70E-53	-1.69E-46
MS.gene060005	MS.gene64414	0.8269	2.10E-54	-1.69E-46
MS.gene06037	MS.gene64414	0.815745	7.90E-52	-1.69E-46
MS.gene060804	MS.gene64414	0.859018	5.53E-63	-1.69E-46
MS.gene060818	MS.gene64414	0.862644	4.38E-64	-1.69E-46
MS.gene061041	MS.gene64414	0.823395	1.42E-53	-1.69E-46
MS.gene061051	MS.gene64414	-0.839724	1.34E-57	-1.69E-46
MS.gene061134	MS.gene64414	0.811893	5.57E-51	-1.69E-46
MS.gene061200	MS.gene64414	0.87278	2.44E-67	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64414.t1	MTR_2g041260	95.000	120	5	1	1	119	1	120	7.67e-60	180
MS.gene64414.t1	MTR_4g125600	64.167	120	37	3	1	118	1	116	6.30e-42	134
MS.gene64414.t1	MTR_1g015290	56.000	125	47	4	1	118	1	124	2.49e-35	118
MS.gene64414.t1	MTR_3g104950	54.918	122	48	4	1	118	1	119	5.09e-35	117

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64414.t1	AT2G06520	55.738	122	43	3	1	118	1	115	1.12e-29	103

Find 40 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGCTTGACGGGATCAAAAT+TGG	0.246839	2.1:-49320041	None:intergenic
CGATACAACTTTCTCCTTCA+TGG	0.258090	2.1:-49319870	None:intergenic
GTTGTGGTTGTAGTTATTAT+TGG	0.278706	2.1:+49320001	MS.gene64414:CDS
GCAATGGAAGATTTCAAGTT+AGG	0.304382	2.1:+49319843	MS.gene64414:CDS
AGCTATTCACCACCCTCTTC+TGG	0.329240	2.1:-49319760	None:intergenic
TTCTTGCTCAGCATTGCAGC+TGG	0.329837	2.1:+49319974	MS.gene64414:CDS
TCTTCGGTCTTCAAAGGCTT+CGG	0.384186	2.1:+49319808	MS.gene64414:CDS
TCTTCAATCCACTTCCTCTT+CGG	0.411814	2.1:+49319792	MS.gene64414:CDS
CAATGGAAGATTTCAAGTTA+GGG	0.418208	2.1:+49319844	MS.gene64414:CDS
ATTTATCAGCGTCGCTTGAC+GGG	0.428873	2.1:-49320052	None:intergenic
TTCTGGCCGGTGTAAGTTAG+TGG	0.442835	2.1:-49319743	None:intergenic
AATTTATCAGCGTCGCTTGA+CGG	0.443913	2.1:-49320053	None:intergenic
CTTGCTCAGCATTGCAGCTG+GGG	0.446840	2.1:+49319976	MS.gene64414:CDS
TTTGAAGACCGAAGAGGAAG+TGG	0.478145	2.1:-49319800	None:intergenic
ATGAAGGAGAAAGTTGTATC+GGG	0.488290	2.1:+49319872	MS.gene64414:CDS
TCTTGCTCAGCATTGCAGCT+GGG	0.491257	2.1:+49319975	MS.gene64414:CDS
ACTTCCTCTTCGGTCTTCAA+AGG	0.493262	2.1:+49319802	MS.gene64414:CDS
CTCCACTTCTGCAGTTTCAG+TGG	0.496724	2.1:+49319715	MS.gene64414:CDS
TTCAATGCTGGTTCCTGATG+TGG	0.502456	2.1:+49319919	MS.gene64414:CDS
AGCATTGACAGCTTCAATGC+TGG	0.508169	2.1:+49319907	MS.gene64414:CDS
TGCCACTGAAACTGCAGAAG+TGG	0.518999	2.1:-49319717	None:intergenic
CATGAAGGAGAAAGTTGTAT+CGG	0.528319	2.1:+49319871	MS.gene64414:CDS
TCAAGTTAGGGCTTCCATGA+AGG	0.532236	2.1:+49319856	MS.gene64414:CDS
TCGGAATCTTTGAAACGCAA+TGG	0.543756	2.1:+49319827	MS.gene64414:CDS
TTGCTCAGCATTGCAGCTGG+GGG	0.544179	2.1:+49319977	MS.gene64414:CDS
AGCAAGAAGTTCTTGAGAGA+AGG	0.544406	2.1:-49319959	None:intergenic
ACGGCCGCTTCAGCCACATC+AGG	0.547544	2.1:-49319932	None:intergenic
ATTCACCACCCTCTTCTGGC+CGG	0.567755	2.1:-49319756	None:intergenic
TAACTTACACCGGCCAGAAG+AGG	0.570657	2.1:+49319747	MS.gene64414:CDS
CACTGAAACTGCAGAAGTGG+AGG	0.579775	2.1:-49319714	None:intergenic
AAGTGGATTGAAGAATGCCT+TGG	0.581435	2.1:-49319783	None:intergenic
GTTCTTGAGAGAAGGTGAAA+CGG	0.587992	2.1:-49319951	None:intergenic
AACTTACACCGGCCAGAAGA+GGG	0.588837	2.1:+49319748	MS.gene64414:CDS
GGTTCCTGATGTGGCTGAAG+CGG	0.602673	2.1:+49319928	MS.gene64414:CDS
GAAGCCTTTGAAGACCGAAG+AGG	0.644109	2.1:-49319806	None:intergenic
GAGGGTGGTGAATAGCTCCA+AGG	0.662303	2.1:+49319766	MS.gene64414:CDS
GCAATGCCACTAACTTACAC+CGG	0.669538	2.1:+49319737	MS.gene64414:CDS
GGTTGTAGTTATTATTGGTG+CGG	0.673405	2.1:+49320006	MS.gene64414:CDS
TGAAGGAGAAAGTTGTATCG+GGG	0.689252	2.1:+49319873	MS.gene64414:CDS
TTACACCGGCCAGAAGAGGG+TGG	0.718271	2.1:+49319751	MS.gene64414:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTTGTGGTTGTAGTTATTAT+TGG	+	chr2.1:49320001-49320020	MS.gene64414:CDS	30.0%
!!	CAATGGAAGATTTCAAGTTA+GGG	+	chr2.1:49319844-49319863	MS.gene64414:CDS	30.0%
	ATGAAGGAGAAAGTTGTATC+GGG	+	chr2.1:49319872-49319891	MS.gene64414:CDS	35.0%
	CATGAAGGAGAAAGTTGTAT+CGG	+	chr2.1:49319871-49319890	MS.gene64414:CDS	35.0%
	GGTTGTAGTTATTATTGGTG+CGG	+	chr2.1:49320006-49320025	MS.gene64414:CDS	35.0%
!!	GCAATGGAAGATTTCAAGTT+AGG	+	chr2.1:49319843-49319862	MS.gene64414:CDS	35.0%
	AGCAAGAAGTTCTTGAGAGA+AGG	-	chr2.1:49319962-49319981	None:intergenic	40.0%
	CGATACAACTTTCTCCTTCA+TGG	-	chr2.1:49319873-49319892	None:intergenic	40.0%
	GTTCTTGAGAGAAGGTGAAA+CGG	-	chr2.1:49319954-49319973	None:intergenic	40.0%
	TCTTCAATCCACTTCCTCTT+CGG	+	chr2.1:49319792-49319811	MS.gene64414:CDS	40.0%
	TGAAGGAGAAAGTTGTATCG+GGG	+	chr2.1:49319873-49319892	MS.gene64414:CDS	40.0%
!!	AAGTGGATTGAAGAATGCCT+TGG	-	chr2.1:49319786-49319805	None:intergenic	40.0%
!!	TCGGAATCTTTGAAACGCAA+TGG	+	chr2.1:49319827-49319846	MS.gene64414:CDS	40.0%
	ACTTCCTCTTCGGTCTTCAA+AGG	+	chr2.1:49319802-49319821	MS.gene64414:CDS	45.0%
	GCAATGCCACTAACTTACAC+CGG	+	chr2.1:49319737-49319756	MS.gene64414:CDS	45.0%
	TCAAGTTAGGGCTTCCATGA+AGG	+	chr2.1:49319856-49319875	MS.gene64414:CDS	45.0%
	TCGCTTGACGGGATCAAAAT+TGG	-	chr2.1:49320044-49320063	None:intergenic	45.0%
	TCTTCGGTCTTCAAAGGCTT+CGG	+	chr2.1:49319808-49319827	MS.gene64414:CDS	45.0%
	TTTGAAGACCGAAGAGGAAG+TGG	-	chr2.1:49319803-49319822	None:intergenic	45.0%
!!	AGCATTGACAGCTTCAATGC+TGG	+	chr2.1:49319907-49319926	MS.gene64414:CDS	45.0%
!!	TTCAATGCTGGTTCCTGATG+TGG	+	chr2.1:49319919-49319938	MS.gene64414:CDS	45.0%
	AACTTACACCGGCCAGAAGA+GGG	+	chr2.1:49319748-49319767	MS.gene64414:CDS	50.0%
	AGCTATTCACCACCCTCTTC+TGG	-	chr2.1:49319763-49319782	None:intergenic	50.0%
	CACTGAAACTGCAGAAGTGG+AGG	-	chr2.1:49319717-49319736	None:intergenic	50.0%
	CTCCACTTCTGCAGTTTCAG+TGG	+	chr2.1:49319715-49319734	MS.gene64414:CDS	50.0%
	TAACTTACACCGGCCAGAAG+AGG	+	chr2.1:49319747-49319766	MS.gene64414:CDS	50.0%
	TCTTGCTCAGCATTGCAGCT+GGG	+	chr2.1:49319975-49319994	MS.gene64414:CDS	50.0%
	TGCCACTGAAACTGCAGAAG+TGG	-	chr2.1:49319720-49319739	None:intergenic	50.0%
	TTCTGGCCGGTGTAAGTTAG+TGG	-	chr2.1:49319746-49319765	None:intergenic	50.0%
	TTCTTGCTCAGCATTGCAGC+TGG	+	chr2.1:49319974-49319993	MS.gene64414:CDS	50.0%
!	GAAGCCTTTGAAGACCGAAG+AGG	-	chr2.1:49319809-49319828	None:intergenic	50.0%
	ATTCACCACCCTCTTCTGGC+CGG	-	chr2.1:49319759-49319778	None:intergenic	55.0%
	CTTGCTCAGCATTGCAGCTG+GGG	+	chr2.1:49319976-49319995	MS.gene64414:CDS	55.0%
	GAGGGTGGTGAATAGCTCCA+AGG	+	chr2.1:49319766-49319785	MS.gene64414:CDS	55.0%
	GGTTCCTGATGTGGCTGAAG+CGG	+	chr2.1:49319928-49319947	MS.gene64414:CDS	55.0%
	TTGCTCAGCATTGCAGCTGG+GGG	+	chr2.1:49319977-49319996	MS.gene64414:CDS	55.0%
	CATTGCAGCTGGGGGAGTTG+TGG	+	chr2.1:49319985-49320004	MS.gene64414:CDS	60.0%
	TTACACCGGCCAGAAGAGGG+TGG	+	chr2.1:49319751-49319770	MS.gene64414:CDS	60.0%
	ACGGCCGCTTCAGCCACATC+AGG	-	chr2.1:49319935-49319954	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	49319710	49320069	49319710	ID=MS.gene64414
chr2.1	mRNA	49319710	49320069	49319710	ID=MS.gene64414.t1;Parent=MS.gene64414
chr2.1	exon	49319710	49320069	49319710	ID=MS.gene64414.t1.exon1;Parent=MS.gene64414.t1
chr2.1	CDS	49319710	49320069	49319710	ID=cds.MS.gene64414.t1;Parent=MS.gene64414.t1

Gene Sequence	>MS.gene64414 ATGGCCTCCACTTCTGCAGTTTCAGTGGCAATGCCACTAACTTACACCGGCCAGAAGAGGGTGGTGAATAGCTCCAAGGCATTCTTCAATCCACTTCCTCTTCGGTCTTCAAAGGCTTCGGAATCTTTGAAACGCAATGGAAGATTTCAAGTTAGGGCTTCCATGAAGGAGAAAGTTGTATCGGGGCTCACAGCAGCAGCATTGACAGCTTCAATGCTGGTTCCTGATGTGGCTGAAGCGGCCGTTTCACCTTCTCTCAAGAACTTCTTGCTCAGCATTGCAGCTGGGGGAGTTGTGGTTGTAGTTATTATTGGTGCGGTGATCGCTGTATCCAATTTTGATCCCGTCAAGCGACGCTGA
Protein sequence	>MS.gene64414.t1 MASTSAVSVAMPLTYTGQKRVVNSSKAFFNPLPLRSSKASESLKRNGRFQVRASMKEKVVSGLTAAALTASMLVPDVAEAAVSPSLKNFLLSIAAGGVVVVVIIGAVIAVSNFDPVKRR