AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71925


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71925.t1 MTR_4g101310 97.458 118 3 0 1 118 17 134 9.85e-79 228
MS.gene71925.t1 MTR_4g101330 49.342 152 42 2 1 117 17 168 5.76e-39 128
MS.gene71925.t1 MTR_4g101280 67.045 88 29 0 31 118 56 143 8.22e-38 125
MS.gene71925.t1 MTR_1g012710 61.628 86 32 1 33 118 67 151 1.57e-34 117
MS.gene71925.t1 MTR_1g012690 56.190 105 37 3 14 118 59 154 1.67e-34 117
MS.gene71925.t1 MTR_1g012700 50.442 113 47 2 14 118 53 164 1.92e-32 112
MS.gene71925.t1 MTR_3g106740 62.353 85 30 2 34 117 46 129 3.57e-31 107
MS.gene71925.t1 MTR_1g012630 58.140 86 35 1 33 118 126 210 7.22e-31 109
MS.gene71925.t1 MTR_4g108150 47.899 119 53 3 1 118 13 123 1.00e-30 106
MS.gene71925.t1 MTR_2g026760 52.101 119 50 5 1 118 15 127 1.23e-30 106
MS.gene71925.t1 MTR_4g101260 58.824 85 34 1 34 118 49 132 1.59e-29 103
MS.gene71925.t1 MTR_5g012340 50.575 87 42 1 31 117 91 176 5.13e-26 96.3
MS.gene71925.t1 MTR_5g012380 50.575 87 42 1 31 117 84 169 6.73e-26 95.5
MS.gene71925.t1 MTR_3g106750 54.762 84 38 0 35 118 46 129 2.58e-25 92.8
MS.gene71925.t1 MTR_4g101270 51.765 85 40 1 33 117 103 186 3.39e-24 91.7
MS.gene71925.t1 MTR_2g026920 44.444 81 42 1 38 118 118 195 6.48e-17 72.8
MS.gene71925.t1 MTR_2g026930 44.444 81 42 1 38 118 122 199 8.89e-17 72.8
MS.gene71925.t1 MTR_3g082100 45.977 87 46 1 31 117 250 335 4.97e-16 72.4
MS.gene71925.t1 MTR_2g026775 48.101 79 35 2 42 118 686 760 5.32e-14 67.0
MS.gene71925.t1 MTR_2g026820 49.367 79 34 3 40 117 542 615 9.88e-14 66.2
MS.gene71925.t1 MTR_4g115360 44.444 90 49 1 29 118 145 233 1.49e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71925.t1 AT1G12100 56.780 118 49 1 1 118 17 132 1.61e-39 129
MS.gene71925.t1 AT1G62510 66.667 84 28 0 34 117 66 149 5.51e-36 120
MS.gene71925.t1 AT4G12500 57.955 88 37 0 31 118 90 177 1.55e-34 117
MS.gene71925.t1 AT4G12490 56.989 93 40 0 26 118 90 182 9.58e-34 115
MS.gene71925.t1 AT4G12520 61.364 88 34 0 31 118 42 129 1.30e-33 114
MS.gene71925.t1 AT4G12510 61.364 88 34 0 31 118 42 129 1.30e-33 114
MS.gene71925.t1 AT2G45180 63.529 85 30 1 34 118 51 134 2.09e-33 113
MS.gene71925.t1 AT4G22460 55.769 104 45 1 13 116 29 131 5.79e-33 112
MS.gene71925.t1 AT1G12090 65.432 81 28 0 37 117 57 137 1.55e-32 111
MS.gene71925.t1 AT4G12480 55.682 88 39 0 31 118 81 168 2.24e-32 112
MS.gene71925.t1 AT4G12470 55.056 89 40 0 30 118 73 161 4.09e-32 111
MS.gene71925.t1 AT4G12550 49.495 99 48 2 21 118 14 111 2.38e-29 102
MS.gene71925.t1 AT4G00165 48.544 103 51 2 16 117 27 128 1.42e-27 98.6
MS.gene71925.t1 AT4G00165 48.544 103 51 2 16 117 27 128 1.42e-27 98.6
MS.gene71925.t1 AT5G46890 54.444 90 38 1 29 118 41 127 2.06e-27 98.2
MS.gene71925.t1 AT4G12530 54.945 91 40 1 27 117 26 115 1.07e-26 95.9
MS.gene71925.t1 AT5G46900 54.444 90 38 1 29 118 41 127 1.13e-26 96.3
MS.gene71925.t1 AT4G12545 49.495 99 45 3 21 118 14 108 5.46e-26 94.0
MS.gene71925.t1 AT3G22120 50.000 86 41 2 33 117 265 349 2.19e-19 82.0
MS.gene71925.t1 AT3G22120 50.000 86 41 2 33 117 248 332 3.63e-19 81.3
MS.gene71925.t1 AT3G22142 50.538 93 43 2 27 117 1387 1478 3.63e-18 79.3
MS.gene71925.t1 AT1G62500 46.429 84 44 1 34 117 211 293 1.04e-17 76.6
MS.gene71925.t1 AT2G10940 44.706 85 46 1 34 118 207 290 1.39e-17 76.3
MS.gene71925.t1 AT2G10940 44.706 85 46 1 34 118 207 290 1.39e-17 76.3
MS.gene71925.t1 AT4G15160 44.186 86 47 1 33 117 97 182 1.06e-16 72.4
MS.gene71925.t1 AT4G15160 44.186 86 47 1 33 117 179 264 3.26e-16 72.4
MS.gene71925.t1 AT4G22490 35.870 92 56 2 26 117 28 116 1.15e-11 57.8

Find 31 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGAGATGCACTTAAAT+TGG 0.207740 8.3:-19882311 MS.gene71925:CDS
AGGATCAAACTTAGTGAAAT+AGG 0.251033 8.3:+19882127 None:intergenic
GTGTGTGCTAATTTGCTTAA+TGG 0.265220 8.3:-19882286 MS.gene71925:CDS
AAGGGAATGGATTTGGATTA+GGG 0.269618 8.3:+19882365 None:intergenic
GCCAAGAATGTTAGCTTTGA+TGG 0.271570 8.3:+19882165 None:intergenic
CTTGGGTAAGGGAATGGATT+TGG 0.286724 8.3:+19882358 None:intergenic
TTTGGATTAGGGTTAGGTTT+TGG 0.310537 8.3:+19882376 None:intergenic
TAAGGGAATGGATTTGGATT+AGG 0.323838 8.3:+19882364 None:intergenic
GCCATCAAAGCTAACATTCT+TGG 0.340537 8.3:-19882166 MS.gene71925:CDS
ATAGAGCAACATGGATGTTC+TGG 0.348478 8.3:+19882235 None:intergenic
GAGTCTTCTCACAAGCATTA+AGG 0.369143 8.3:+19882107 None:intergenic
AATGGATTTGGATTAGGGTT+AGG 0.406983 8.3:+19882370 None:intergenic
GAAGGACTTGTGCTTGGGTA+AGG 0.431525 8.3:+19882346 None:intergenic
CCAAGAGATGCACTTAAATT+GGG 0.431689 8.3:-19882310 MS.gene71925:CDS
GGACCAATTGGTGCAGTGAT+TGG 0.437596 8.3:-19882265 MS.gene71925:CDS
CCCAATTTAAGTGCATCTCT+TGG 0.455798 8.3:+19882310 None:intergenic
GACCCAATCACTGCACCAAT+TGG 0.459457 8.3:+19882262 None:intergenic
TAGTGGAAGGACTTGTGCTT+GGG 0.461750 8.3:+19882341 None:intergenic
AATTTGCTTAATGGACCAAT+TGG 0.477291 8.3:-19882277 MS.gene71925:CDS
ATCTCTTGGACAACTCTTAG+TGG 0.495008 8.3:+19882324 None:intergenic
TTAGTGGAAGGACTTGTGCT+TGG 0.502701 8.3:+19882340 None:intergenic
CTTGTGCTTGGGTAAGGGAA+TGG 0.504980 8.3:+19882352 None:intergenic
GACCAATTGGTGCAGTGATT+GGG 0.509536 8.3:-19882264 MS.gene71925:CDS
TAACACTTAATTAAGAGCAC+TGG 0.528966 8.3:+19882071 None:intergenic
AAGGACTTGTGCTTGGGTAA+GGG 0.591360 8.3:+19882347 None:intergenic
TAAGAGCACTGGAAATCAGT+GGG 0.619022 8.3:+19882082 None:intergenic
CTTGGACAACTCTTAGTGGA+AGG 0.619262 8.3:+19882328 None:intergenic
CCTTCTAAAATAGAGCAACA+TGG 0.620844 8.3:+19882226 None:intergenic
TTAAGAGCACTGGAAATCAG+TGG 0.653235 8.3:+19882081 None:intergenic
GAGCACTGGAAATCAGTGGG+TGG 0.693320 8.3:+19882085 None:intergenic
GAGCAACATGGATGTTCTGG+TGG 0.695269 8.3:+19882238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGGATCAAACTTAGTGAAAT+AGG + chr8.3:19882372-19882391 None:intergenic 30.0%
! AATTTGCTTAATGGACCAAT+TGG - chr8.3:19882219-19882238 MS.gene71925:CDS 30.0%
CCAAGAGATGCACTTAAATT+GGG - chr8.3:19882186-19882205 MS.gene71925:CDS 35.0%
TCCAAGAGATGCACTTAAAT+TGG - chr8.3:19882185-19882204 MS.gene71925:CDS 35.0%
! AAGGGAATGGATTTGGATTA+GGG + chr8.3:19882134-19882153 None:intergenic 35.0%
! AATGGATTTGGATTAGGGTT+AGG + chr8.3:19882129-19882148 None:intergenic 35.0%
! CTTTTCTTTGCAATGGCAAA+TGG - chr8.3:19882084-19882103 MS.gene71925:CDS 35.0%
! TAAGGGAATGGATTTGGATT+AGG + chr8.3:19882135-19882154 None:intergenic 35.0%
!! CCATGTTGCTCTATTTTAGA+AGG - chr8.3:19882270-19882289 MS.gene71925:CDS 35.0%
!! CCTTCTAAAATAGAGCAACA+TGG + chr8.3:19882273-19882292 None:intergenic 35.0%
!! GTGTGTGCTAATTTGCTTAA+TGG - chr8.3:19882210-19882229 MS.gene71925:CDS 35.0%
!! TTTGGATTAGGGTTAGGTTT+TGG + chr8.3:19882123-19882142 None:intergenic 35.0%
ATAGAGCAACATGGATGTTC+TGG + chr8.3:19882264-19882283 None:intergenic 40.0%
ATCTCTTGGACAACTCTTAG+TGG + chr8.3:19882175-19882194 None:intergenic 40.0%
CCCAATTTAAGTGCATCTCT+TGG + chr8.3:19882189-19882208 None:intergenic 40.0%
GAGTCTTCTCACAAGCATTA+AGG + chr8.3:19882392-19882411 None:intergenic 40.0%
GCCATCAAAGCTAACATTCT+TGG - chr8.3:19882330-19882349 MS.gene71925:CDS 40.0%
!! GCCAAGAATGTTAGCTTTGA+TGG + chr8.3:19882334-19882353 None:intergenic 40.0%
!! TAAGAGCACTGGAAATCAGT+GGG + chr8.3:19882417-19882436 None:intergenic 40.0%
!! TTAAGAGCACTGGAAATCAG+TGG + chr8.3:19882418-19882437 None:intergenic 40.0%
AAGGACTTGTGCTTGGGTAA+GGG + chr8.3:19882152-19882171 None:intergenic 45.0%
CTTGGACAACTCTTAGTGGA+AGG + chr8.3:19882171-19882190 None:intergenic 45.0%
GACCAATTGGTGCAGTGATT+GGG - chr8.3:19882232-19882251 MS.gene71925:CDS 45.0%
TAGTGGAAGGACTTGTGCTT+GGG + chr8.3:19882158-19882177 None:intergenic 45.0%
TTAGTGGAAGGACTTGTGCT+TGG + chr8.3:19882159-19882178 None:intergenic 45.0%
! CTTGGGTAAGGGAATGGATT+TGG + chr8.3:19882141-19882160 None:intergenic 45.0%
CTTGTGCTTGGGTAAGGGAA+TGG + chr8.3:19882147-19882166 None:intergenic 50.0%
GAAGGACTTGTGCTTGGGTA+AGG + chr8.3:19882153-19882172 None:intergenic 50.0%
GACCCAATCACTGCACCAAT+TGG + chr8.3:19882237-19882256 None:intergenic 50.0%
GAGCAACATGGATGTTCTGG+TGG + chr8.3:19882261-19882280 None:intergenic 50.0%
GGACCAATTGGTGCAGTGAT+TGG - chr8.3:19882231-19882250 MS.gene71925:CDS 50.0%
!! GAGCACTGGAAATCAGTGGG+TGG + chr8.3:19882414-19882433 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 19882081 19882437 19882081 ID=MS.gene71925
chr8.3 mRNA 19882081 19882437 19882081 ID=MS.gene71925.t1;Parent=MS.gene71925
chr8.3 exon 19882081 19882437 19882081 ID=MS.gene71925.t1.exon1;Parent=MS.gene71925.t1
chr8.3 CDS 19882081 19882437 19882081 ID=cds.MS.gene71925.t1;Parent=MS.gene71925.t1
Gene Sequence

>MS.gene71925

ATGCTTTTCTTTGCAATGGCAAATGGTTGTTTTTTTTGTCCAAAACCTAACCCTAATCCAAATCCATTCCCTTACCCAAGCACAAGTCCTTCCACTAAGAGTTGTCCAAGAGATGCACTTAAATTGGGAGTGTGTGCTAATTTGCTTAATGGACCAATTGGTGCAGTGATTGGGTCACCACCAGAACATCCATGTTGCTCTATTTTAGAAGGACTTGTTGATCTTGAAGTTGCAGTTTGTCTTTGCACTGCCATCAAAGCTAACATTCTTGGCATTAATATTAATATTCCTATTTCACTAAGTTTGATCCTTAATGCTTGTGAGAAGACTCCACCCACTGATTTCCAGTGCTCTTAA

Protein sequence

>MS.gene71925.t1

MLFFAMANGCFFCPKPNPNPNPFPYPSTSPSTKSCPRDALKLGVCANLLNGPIGAVIGSPPEHPCCSILEGLVDLEVAVCLCTAIKANILGININIPISLSLILNACEKTPPTDFQCS