AlfalfaGEDB | MS.gene83687

M. sativa cultivar XinJiangDaYe / MS.gene83687

KEGG: K02183
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF13499.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83687.t1	XP_003612498.1	95.5	200	9	0	1	200	1	200	1.80E-85	325.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83687.t1	Q93Z27	40.1	182	95	5	1	173	1	177	2.8e-23	110.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83687.t1	G7K424	95.5	200	9	0	1	200	1	200	1.3e-85	325.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene83687	0.860393	2.13E-63	-1.69E-46
MS.gene049419	MS.gene83687	-0.804325	2.28E-49	-1.69E-46
MS.gene049499	MS.gene83687	0.809809	1.57E-50	-1.69E-46
MS.gene049861	MS.gene83687	0.823723	1.19E-53	-1.69E-46
MS.gene049983	MS.gene83687	0.835014	2.15E-56	-1.69E-46
MS.gene04998	MS.gene83687	0.843921	1.04E-58	-1.69E-46
MS.gene050359	MS.gene83687	0.802643	5.09E-49	-1.69E-46
MS.gene050646	MS.gene83687	0.80637	8.50E-50	-1.69E-46
MS.gene050654	MS.gene83687	0.820531	6.53E-53	-1.69E-46
MS.gene051122	MS.gene83687	0.814055	1.87E-51	-1.69E-46
MS.gene051184	MS.gene83687	0.841479	4.63E-58	-1.69E-46
MS.gene051275	MS.gene83687	0.805822	1.11E-49	-1.69E-46
MS.gene051541	MS.gene83687	0.827207	1.78E-54	-1.69E-46
MS.gene051655	MS.gene83687	0.800831	1.20E-48	-1.69E-46
MS.gene051831	MS.gene83687	0.819324	1.23E-52	-1.69E-46
MS.gene052296	MS.gene83687	0.801198	1.01E-48	-1.69E-46
MS.gene052533	MS.gene83687	0.80978	1.60E-50	-1.69E-46
MS.gene052685	MS.gene83687	0.810737	9.94E-51	-1.69E-46
MS.gene052932	MS.gene83687	0.800495	1.40E-48	-1.69E-46
MS.gene053094	MS.gene83687	0.814385	1.58E-51	-1.69E-46
MS.gene053298	MS.gene83687	0.854886	9.13E-62	-1.69E-46
MS.gene053340	MS.gene83687	0.804026	2.63E-49	-1.69E-46
MS.gene053456	MS.gene83687	0.826171	3.14E-54	-1.69E-46
MS.gene053564	MS.gene83687	0.803805	2.93E-49	-1.69E-46
MS.gene053575	MS.gene83687	0.803453	3.46E-49	-1.69E-46
MS.gene053726	MS.gene83687	0.85067	1.46E-60	-1.69E-46
MS.gene053879	MS.gene83687	0.833344	5.63E-56	-1.69E-46
MS.gene053963	MS.gene83687	0.859365	4.35E-63	-1.69E-46
MS.gene054222	MS.gene83687	0.820097	8.21E-53	-1.69E-46
MS.gene05439	MS.gene83687	0.827669	1.38E-54	-1.69E-46
MS.gene054479	MS.gene83687	0.830385	3.03E-55	-1.69E-46
MS.gene054892	MS.gene83687	0.88529	9.24E-72	-1.69E-46
MS.gene054893	MS.gene83687	0.88956	2.17E-73	-1.69E-46
MS.gene054957	MS.gene83687	0.817473	3.24E-52	-1.69E-46
MS.gene055147	MS.gene83687	0.812932	3.31E-51	-1.69E-46
MS.gene055149	MS.gene83687	0.80089	1.17E-48	-1.69E-46
MS.gene055150	MS.gene83687	0.82286	1.89E-53	-1.69E-46
MS.gene055222	MS.gene83687	0.827849	1.25E-54	-1.69E-46
MS.gene055226	MS.gene83687	0.812856	3.44E-51	-1.69E-46
MS.gene055442	MS.gene83687	0.820434	6.87E-53	-1.69E-46
MS.gene05624	MS.gene83687	0.828414	9.11E-55	-1.69E-46
MS.gene056699	MS.gene83687	0.801222	9.97E-49	-1.69E-46
MS.gene056849	MS.gene83687	0.816377	5.71E-52	-1.69E-46
MS.gene056986	MS.gene83687	0.802317	5.94E-49	-1.69E-46
MS.gene057102	MS.gene83687	0.806794	6.92E-50	-1.69E-46
MS.gene057155	MS.gene83687	0.856957	2.26E-62	-1.69E-46
MS.gene057622	MS.gene83687	0.85616	3.88E-62	-1.69E-46
MS.gene057999	MS.gene83687	0.809934	1.48E-50	-1.69E-46
MS.gene058095	MS.gene83687	0.800042	1.73E-48	-1.69E-46
MS.gene058159	MS.gene83687	0.847268	1.29E-59	-1.69E-46
MS.gene058429	MS.gene83687	0.846936	1.59E-59	-1.69E-46
MS.gene058592	MS.gene83687	0.831088	2.03E-55	-1.69E-46
MS.gene05878	MS.gene83687	0.856207	3.76E-62	-1.69E-46
MS.gene05952	MS.gene83687	0.892528	1.46E-74	-1.69E-46
MS.gene05956	MS.gene83687	0.828973	6.68E-55	-1.69E-46
MS.gene05985	MS.gene83687	-0.811497	6.80E-51	-1.69E-46
MS.gene06018	MS.gene83687	0.801827	7.49E-49	-1.69E-46
MS.gene06023	MS.gene83687	0.833579	4.92E-56	-1.69E-46
MS.gene060259	MS.gene83687	0.841271	5.26E-58	-1.69E-46
MS.gene060412	MS.gene83687	0.834211	3.42E-56	-1.69E-46
MS.gene060413	MS.gene83687	0.825944	3.56E-54	-1.69E-46
MS.gene060840	MS.gene83687	0.815908	7.26E-52	-1.69E-46
MS.gene060850	MS.gene83687	0.825179	5.40E-54	-1.69E-46
MS.gene060851	MS.gene83687	0.836236	1.05E-56	-1.69E-46
MS.gene060852	MS.gene83687	0.835964	1.23E-56	-1.69E-46
MS.gene060932	MS.gene83687	0.827647	1.39E-54	-1.69E-46
MS.gene060933	MS.gene83687	0.813024	3.16E-51	-1.69E-46
MS.gene060937	MS.gene83687	0.822193	2.70E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83687.t1	MTR_5g025690	97.000	200	6	0	1	200	1	200	1.42e-137	383
MS.gene83687.t1	MTR_7g011010	81.068	206	29	2	1	200	1	202	2.72e-104	298
MS.gene83687.t1	MTR_4g127560	68.132	182	50	2	27	200	17	198	2.60e-76	228
MS.gene83687.t1	MTR_2g023310	84.314	51	8	0	83	133	47	97	1.86e-22	87.4
MS.gene83687.t1	MTR_4g082050	42.520	127	68	4	71	196	17	139	1.23e-21	86.7
MS.gene83687.t1	MTR_7g074240	53.846	65	30	0	131	195	106	170	6.51e-16	72.4
MS.gene83687.t1	MTR_4g115170	44.595	74	41	0	124	197	145	218	9.24e-14	67.8
MS.gene83687.t1	MTR_2g098890	27.461	193	115	5	29	196	11	203	1.18e-13	67.0
MS.gene83687.t1	MTR_4g067270	34.921	126	68	4	85	197	19	143	5.50e-13	63.9
MS.gene83687.t1	MTR_7g089760	36.000	125	66	3	85	196	19	142	7.74e-13	63.9
MS.gene83687.t1	MTR_8g107110	31.293	147	66	5	66	197	33	159	2.83e-12	62.4
MS.gene83687.t1	MTR_3g109320	36.667	120	67	2	84	195	60	178	1.07e-11	61.2
MS.gene83687.t1	MTR_3g067610	35.000	120	66	4	85	195	19	135	3.10e-11	59.3
MS.gene83687.t1	MTR_1g032070	37.600	125	60	5	85	195	60	180	4.89e-11	59.7
MS.gene83687.t1	MTR_3g088655	43.056	72	41	0	124	195	83	154	8.57e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83687.t1	AT5G39670	43.541	209	104	5	1	200	1	204	4.39e-45	148
MS.gene83687.t1	AT3G29000	61.111	108	40	1	89	196	85	190	1.95e-38	131
MS.gene83687.t1	AT4G20780	57.746	71	27	1	125	195	116	183	1.25e-17	77.4
MS.gene83687.t1	AT3G47480	56.061	66	28	1	131	196	115	179	6.14e-17	75.5
MS.gene83687.t1	AT2G43290	32.308	130	69	2	85	196	79	207	1.61e-15	72.4
MS.gene83687.t1	AT5G44460	48.276	87	42	1	110	196	92	175	2.11e-15	71.2
MS.gene83687.t1	AT3G59440	28.421	190	107	5	29	197	7	188	8.48e-15	70.1
MS.gene83687.t1	AT1G05990	47.692	65	34	0	133	197	80	144	3.47e-14	67.4
MS.gene83687.t1	AT3G07490	45.161	62	34	0	136	197	82	143	6.39e-13	63.9
MS.gene83687.t1	AT4G03290	46.875	64	34	0	133	196	82	145	8.19e-13	63.9
MS.gene83687.t1	AT4G12860	36.036	111	67	3	87	197	37	143	1.26e-11	60.5
MS.gene83687.t1	AT1G21550	34.234	111	66	2	85	191	41	148	5.32e-11	58.9

Find 24 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGAGTTATGTGTATTT+TGG	0.141163	5.4:+16791038	MS.gene83687:CDS
ACTTCATGTTCAACTTTCTT+TGG	0.236848	5.4:+16790649	MS.gene83687:CDS
TGAAACGGTGATGGCGAAAA+TGG	0.238809	5.4:+16790855	MS.gene83687:CDS
GAGAAAGTGGAGAGATTAAG+AGG	0.257266	5.4:+16790824	MS.gene83687:CDS
TGAAATCAATTCTTCCATCT+TGG	0.401363	5.4:-16791122	None:intergenic
TGATGAGAATGAGCCAAGTT+TGG	0.401446	5.4:+16790948	MS.gene83687:CDS
ATCTTCACTTCCTCCAAACT+TGG	0.413419	5.4:-16790961	None:intergenic
CTTCTCTGCCAACTTCGTTC+CGG	0.421099	5.4:+16790745	MS.gene83687:CDS
AGAAGTAAAGGGAGAGAAGC+AGG	0.460203	5.4:+16790774	MS.gene83687:CDS
CTAAGTCGCCGGAACGAAGT+TGG	0.464546	5.4:-16790753	None:intergenic
TTCCGGCGACTTAGAAGTAA+AGG	0.467315	5.4:+16790762	MS.gene83687:CDS
TCCCTTTACTTCTAAGTCGC+CGG	0.486137	5.4:-16790764	None:intergenic
TCAAATGTTAGCAGAGAAAG+TGG	0.493623	5.4:+16790811	MS.gene83687:CDS
GAAACGGTGATGGCGAAAAT+GGG	0.493859	5.4:+16790856	MS.gene83687:CDS
AAGTTTGGAGGAAGTGAAGA+TGG	0.496634	5.4:+16790963	MS.gene83687:CDS
AGAAAGTGGAGAGATTAAGA+GGG	0.503717	5.4:+16790825	MS.gene83687:CDS
TGAGAATGAGCCAAGTTTGG+AGG	0.510803	5.4:+16790951	MS.gene83687:CDS
CGGAACGAAGTTGGCAGAGA+AGG	0.517501	5.4:-16790744	None:intergenic
AGATGGATTTATTGATGCAA+AGG	0.523959	5.4:+16791011	MS.gene83687:CDS
TAAGAGGGATGAAGTTGAAA+CGG	0.526266	5.4:+16790840	MS.gene83687:CDS
ACTTGTTTGTCTTATTGAAG+AGG	0.531071	5.4:+16790594	MS.gene83687:CDS
CTTCAATAAGACAAACAAGT+TGG	0.539471	5.4:-16790591	None:intergenic
TCCGGCGACTTAGAAGTAAA+GGG	0.551355	5.4:+16790763	MS.gene83687:CDS
GGATGAAGTTGAAACGGTGA+TGG	0.589256	5.4:+16790846	MS.gene83687:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CACTGAATTTGTAAAAATTA+TGG	+	chr5.4:16791143-16791162	MS.gene83687:CDS	20.0%
!!!	AAAAGTTTTTCTCAACTTTT+TGG	+	chr5.4:16790716-16790735	MS.gene83687:CDS	20.0%
!	GATGAACTTGATGAAAAATA+TGG	+	chr5.4:16790901-16790920	MS.gene83687:CDS	25.0%
!!	CAAAGAGTTATGTGTATTTT+GGG	+	chr5.4:16791039-16791058	MS.gene83687:CDS	25.0%
!!!	AATTTTGATGAAAACCAAGA+TGG	+	chr5.4:16791108-16791127	MS.gene83687:CDS	25.0%
	ACTTCATGTTCAACTTTCTT+TGG	+	chr5.4:16790649-16790668	MS.gene83687:CDS	30.0%
	CTTCAATAAGACAAACAAGT+TGG	-	chr5.4:16790594-16790613	None:intergenic	30.0%
	TGAAATCAATTCTTCCATCT+TGG	-	chr5.4:16791125-16791144	None:intergenic	30.0%
!	ACTTGTTTGTCTTATTGAAG+AGG	+	chr5.4:16790594-16790613	MS.gene83687:CDS	30.0%
!	AGATGGATTTATTGATGCAA+AGG	+	chr5.4:16791011-16791030	MS.gene83687:CDS	30.0%
!	GCAAAGAGTTATGTGTATTT+TGG	+	chr5.4:16791038-16791057	MS.gene83687:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	chr5.4:16790994-16791013	MS.gene83687:CDS	30.0%
!!!	TATGTGTATTTTGGGATTGA+AGG	+	chr5.4:16791047-16791066	MS.gene83687:CDS	30.0%
	AGAAAGTGGAGAGATTAAGA+GGG	+	chr5.4:16790825-16790844	MS.gene83687:CDS	35.0%
	TAAGAGGGATGAAGTTGAAA+CGG	+	chr5.4:16790840-16790859	MS.gene83687:CDS	35.0%
	TCAAATGTTAGCAGAGAAAG+TGG	+	chr5.4:16790811-16790830	MS.gene83687:CDS	35.0%
!!!	TGTATTTTGGGATTGAAGGA+AGG	+	chr5.4:16791051-16791070	MS.gene83687:CDS	35.0%
	AAGTTTGGAGGAAGTGAAGA+TGG	+	chr5.4:16790963-16790982	MS.gene83687:CDS	40.0%
	ATCTTCACTTCCTCCAAACT+TGG	-	chr5.4:16790964-16790983	None:intergenic	40.0%
	GAGAAAGTGGAGAGATTAAG+AGG	+	chr5.4:16790824-16790843	MS.gene83687:CDS	40.0%
	TGATGAGAATGAGCCAAGTT+TGG	+	chr5.4:16790948-16790967	MS.gene83687:CDS	40.0%
	AGAAGTAAAGGGAGAGAAGC+AGG	+	chr5.4:16790774-16790793	MS.gene83687:CDS	45.0%
	GGATGAAGTTGAAACGGTGA+TGG	+	chr5.4:16790846-16790865	MS.gene83687:CDS	45.0%
	TCCCTTTACTTCTAAGTCGC+CGG	-	chr5.4:16790767-16790786	None:intergenic	45.0%
	TCCGGCGACTTAGAAGTAAA+GGG	+	chr5.4:16790763-16790782	MS.gene83687:CDS	45.0%
	TGAGAATGAGCCAAGTTTGG+AGG	+	chr5.4:16790951-16790970	MS.gene83687:CDS	45.0%
	TTCCGGCGACTTAGAAGTAA+AGG	+	chr5.4:16790762-16790781	MS.gene83687:CDS	45.0%
!	GAAACGGTGATGGCGAAAAT+GGG	+	chr5.4:16790856-16790875	MS.gene83687:CDS	45.0%
!	TGAAACGGTGATGGCGAAAA+TGG	+	chr5.4:16790855-16790874	MS.gene83687:CDS	45.0%
	CTTCTCTGCCAACTTCGTTC+CGG	+	chr5.4:16790745-16790764	MS.gene83687:CDS	50.0%
	CGGAACGAAGTTGGCAGAGA+AGG	-	chr5.4:16790747-16790766	None:intergenic	55.0%
	CTAAGTCGCCGGAACGAAGT+TGG	-	chr5.4:16790756-16790775	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	16790580	16791182	16790580	ID=MS.gene83687
chr5.4	mRNA	16790580	16791182	16790580	ID=MS.gene83687.t1;Parent=MS.gene83687
chr5.4	exon	16790580	16791182	16790580	ID=MS.gene83687.t1.exon1;Parent=MS.gene83687.t1
chr5.4	CDS	16790580	16791182	16790580	ID=cds.MS.gene83687.t1;Parent=MS.gene83687.t1

Gene Sequence

>MS.gene83687

ATGGTTTTTAGCCAACTTGTTTGTCTTATTGAAGAGGAGAAGACATCACAGAATGATGATAGCAACTCAACTTCATGTTCAACTTTCTTTGGTTTGATAGATTTTTTCATATATTCAACATTATTCATCAGAATACAAAAGTTTTTCTCAACTTTTTGGTTTTTCCTTCTCTGCCAACTTCGTTCCGGCGACTTAGAAGTAAAGGGAGAGAAGCAGGTATCAGAAACTGAATCAAATGTTAGCAGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAACGGTGATGGCGAAAATGGGAATTTTTTGCAGCTCAGAAAGTGATGAACTTGATGAAAAATATGGTTCAAATGAACTTTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTTGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAAAGGAATTGCAAAGAGTTATGTGTATTTTGGGATTGAAGGAAGGTTCAGAAGTTAAGAATTGTCAGAAGATGATCAAGAATTTTGATGAAAACCAAGATGGAAGAATTGATTTCACTGAATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>MS.gene83687.t1

MVFSQLVCLIEEEKTSQNDDSNSTSCSTFFGLIDFFIYSTLFIRIQKFFSTFWFFLLCQLRSGDLEVKGEKQVSETESNVSRESGEIKRDEVETVMAKMGIFCSSESDELDEKYGSNELSELFDENEPSLEEVKMAFDVFDENKDGFIDAKELQRVMCILGLKEGSEVKNCQKMIKNFDENQDGRIDFTEFVKIMENRLC