Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87222.t1 | XP_013455618.1 | 97.5 | 326 | 8 | 0 | 1 | 326 | 1 | 326 | 3.00E-183 | 651 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87222.t1 | Q9SUT2 | 61.0 | 318 | 122 | 2 | 11 | 326 | 9 | 326 | 5.8e-111 | 402.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87222.t1 | A0A072UIQ7 | 97.5 | 326 | 8 | 0 | 1 | 326 | 1 | 326 | 2.1e-183 | 651.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene87222 | 0.838776 | 2.35E-57 | -1.69E-46 |
| MS.gene049549 | MS.gene87222 | 0.853001 | 3.19E-61 | -1.69E-46 |
| MS.gene050095 | MS.gene87222 | 0.8035 | 3.39E-49 | -1.69E-46 |
| MS.gene050096 | MS.gene87222 | 0.802225 | 6.21E-49 | -1.69E-46 |
| MS.gene050329 | MS.gene87222 | 0.821794 | 3.34E-53 | -1.69E-46 |
| MS.gene051131 | MS.gene87222 | 0.837619 | 4.67E-57 | -1.69E-46 |
| MS.gene051996 | MS.gene87222 | 0.814439 | 1.54E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene87222 | 0.823104 | 1.66E-53 | -1.69E-46 |
| MS.gene052223 | MS.gene87222 | 0.840082 | 1.08E-57 | -1.69E-46 |
| MS.gene05240 | MS.gene87222 | 0.837414 | 5.27E-57 | -1.69E-46 |
| MS.gene05244 | MS.gene87222 | 0.811763 | 5.95E-51 | -1.69E-46 |
| MS.gene052702 | MS.gene87222 | 0.805069 | 1.60E-49 | -1.69E-46 |
| MS.gene052706 | MS.gene87222 | 0.824031 | 1.01E-53 | -1.69E-46 |
| MS.gene053406 | MS.gene87222 | 0.827331 | 1.66E-54 | -1.69E-46 |
| MS.gene054146 | MS.gene87222 | 0.832171 | 1.10E-55 | -1.69E-46 |
| MS.gene054181 | MS.gene87222 | 0.809958 | 1.46E-50 | -1.69E-46 |
| MS.gene054277 | MS.gene87222 | 0.866259 | 3.25E-65 | -1.69E-46 |
| MS.gene054290 | MS.gene87222 | 0.80229 | 6.02E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene87222 | 0.857144 | 1.99E-62 | -1.69E-46 |
| MS.gene055240 | MS.gene87222 | 0.822699 | 2.06E-53 | -1.69E-46 |
| MS.gene055601 | MS.gene87222 | 0.813431 | 2.57E-51 | -1.69E-46 |
| MS.gene055959 | MS.gene87222 | 0.809274 | 2.05E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene87222 | 0.842348 | 2.73E-58 | -1.69E-46 |
| MS.gene056590 | MS.gene87222 | 0.822875 | 1.87E-53 | -1.69E-46 |
| MS.gene056591 | MS.gene87222 | 0.822119 | 2.81E-53 | -1.69E-46 |
| MS.gene056680 | MS.gene87222 | 0.803837 | 2.88E-49 | -1.69E-46 |
| MS.gene056690 | MS.gene87222 | 0.812958 | 3.26E-51 | -1.69E-46 |
| MS.gene056691 | MS.gene87222 | 0.82822 | 1.01E-54 | -1.69E-46 |
| MS.gene056697 | MS.gene87222 | 0.818626 | 1.78E-52 | -1.69E-46 |
| MS.gene056715 | MS.gene87222 | 0.813806 | 2.12E-51 | -1.69E-46 |
| MS.gene056899 | MS.gene87222 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene87222 | 0.86865 | 5.58E-66 | -1.69E-46 |
| MS.gene056922 | MS.gene87222 | 0.826459 | 2.68E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene87222 | 0.86028 | 2.31E-63 | -1.69E-46 |
| MS.gene056924 | MS.gene87222 | 0.853505 | 2.29E-61 | -1.69E-46 |
| MS.gene056925 | MS.gene87222 | 0.809115 | 2.22E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene87222 | 0.810151 | 1.33E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene87222 | 0.842915 | 1.93E-58 | -1.69E-46 |
| MS.gene057547 | MS.gene87222 | 0.813254 | 2.81E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene87222 | 0.80203 | 6.81E-49 | -1.69E-46 |
| MS.gene058177 | MS.gene87222 | 0.803752 | 3.00E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene87222 | 0.805722 | 1.16E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene87222 | 0.801951 | 7.07E-49 | -1.69E-46 |
| MS.gene059328 | MS.gene87222 | 0.807394 | 5.16E-50 | -1.69E-46 |
| MS.gene059402 | MS.gene87222 | 0.816775 | 4.65E-52 | -1.69E-46 |
| MS.gene059953 | MS.gene87222 | 0.814235 | 1.71E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene87222 | 0.832107 | 1.14E-55 | -1.69E-46 |
| MS.gene060087 | MS.gene87222 | 0.862145 | 6.23E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87222 | MS.gene029047 | PPI |
| MS.gene87222 | MS.gene32715 | PPI |
| MS.gene87222 | MS.gene009198 | PPI |
| MS.gene87222 | MS.gene71470 | PPI |
| MS.gene87222 | MS.gene35795 | PPI |
| MS.gene87222 | MS.gene35931 | PPI |
| MS.gene87222 | MS.gene038655 | PPI |
| MS.gene87222 | MS.gene69473 | PPI |
| MS.gene87222 | MS.gene014924 | PPI |
| MS.gene87222 | MS.gene35853 | PPI |
| MS.gene87222 | MS.gene80170 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87222.t1 | MTR_4g046713 | 97.546 | 326 | 8 | 0 | 1 | 326 | 1 | 326 | 0.0 | 667 |
| MS.gene87222.t1 | MTR_2g088770 | 71.341 | 328 | 89 | 4 | 1 | 326 | 1 | 325 | 6.66e-177 | 493 |
| MS.gene87222.t1 | MTR_7g107520 | 54.430 | 316 | 138 | 4 | 11 | 326 | 11 | 320 | 8.50e-122 | 353 |
| MS.gene87222.t1 | MTR_3g092990 | 46.082 | 319 | 171 | 1 | 9 | 326 | 8 | 326 | 1.04e-97 | 291 |
| MS.gene87222.t1 | MTR_5g017860 | 43.425 | 327 | 183 | 2 | 1 | 326 | 1 | 326 | 5.47e-94 | 282 |
| MS.gene87222.t1 | MTR_5g017850 | 42.508 | 327 | 186 | 2 | 1 | 326 | 1 | 326 | 1.91e-93 | 281 |
| MS.gene87222.t1 | MTR_2g029820 | 44.411 | 331 | 176 | 4 | 3 | 326 | 1 | 330 | 2.09e-93 | 282 |
| MS.gene87222.t1 | MTR_5g021060 | 48.344 | 302 | 153 | 3 | 27 | 326 | 30 | 330 | 7.10e-91 | 275 |
| MS.gene87222.t1 | MTR_2g029800 | 44.237 | 321 | 171 | 4 | 13 | 326 | 11 | 330 | 1.31e-90 | 275 |
| MS.gene87222.t1 | MTR_5g022870 | 47.170 | 318 | 156 | 6 | 12 | 326 | 8 | 316 | 2.08e-90 | 273 |
| MS.gene87222.t1 | MTR_2g029815 | 43.807 | 331 | 178 | 4 | 3 | 326 | 1 | 330 | 2.28e-90 | 274 |
| MS.gene87222.t1 | MTR_1g101830 | 43.731 | 327 | 173 | 6 | 3 | 326 | 4 | 322 | 7.47e-90 | 271 |
| MS.gene87222.t1 | MTR_2g008710 | 45.260 | 327 | 167 | 5 | 7 | 326 | 4 | 325 | 2.21e-89 | 270 |
| MS.gene87222.t1 | MTR_2g029850 | 44.548 | 321 | 170 | 5 | 13 | 326 | 11 | 330 | 5.03e-89 | 270 |
| MS.gene87222.t1 | MTR_2g040000 | 46.254 | 307 | 156 | 5 | 26 | 326 | 28 | 331 | 5.75e-88 | 267 |
| MS.gene87222.t1 | MTR_8g075100 | 45.246 | 305 | 161 | 4 | 22 | 326 | 21 | 319 | 8.35e-88 | 266 |
| MS.gene87222.t1 | MTR_2g028980 | 42.598 | 331 | 182 | 4 | 3 | 326 | 1 | 330 | 2.33e-87 | 266 |
| MS.gene87222.t1 | MTR_2g029740 | 43.902 | 328 | 173 | 5 | 9 | 326 | 4 | 330 | 3.15e-87 | 266 |
| MS.gene87222.t1 | MTR_1g054205 | 41.049 | 324 | 186 | 4 | 7 | 326 | 4 | 326 | 1.62e-86 | 263 |
| MS.gene87222.t1 | MTR_7g093370 | 41.159 | 328 | 188 | 4 | 3 | 326 | 45 | 371 | 1.74e-86 | 265 |
| MS.gene87222.t1 | MTR_2g029830 | 45.130 | 308 | 165 | 3 | 22 | 326 | 23 | 329 | 1.82e-86 | 264 |
| MS.gene87222.t1 | MTR_3g094650 | 41.534 | 313 | 172 | 4 | 13 | 322 | 14 | 318 | 2.55e-86 | 263 |
| MS.gene87222.t1 | MTR_0155s0040 | 43.974 | 307 | 161 | 4 | 20 | 326 | 20 | 315 | 8.02e-86 | 261 |
| MS.gene87222.t1 | MTR_2g029560 | 42.813 | 327 | 182 | 5 | 3 | 326 | 1 | 325 | 1.14e-85 | 261 |
| MS.gene87222.t1 | MTR_3g092990 | 43.887 | 319 | 167 | 2 | 9 | 326 | 8 | 315 | 2.15e-85 | 260 |
| MS.gene87222.t1 | MTR_1g038680 | 46.382 | 304 | 157 | 5 | 28 | 326 | 30 | 332 | 5.97e-85 | 259 |
| MS.gene87222.t1 | MTR_1g077000 | 41.250 | 320 | 177 | 3 | 7 | 326 | 19 | 327 | 7.88e-85 | 259 |
| MS.gene87222.t1 | MTR_2g029750 | 43.202 | 331 | 180 | 4 | 3 | 326 | 1 | 330 | 1.91e-84 | 259 |
| MS.gene87222.t1 | MTR_4g125940 | 42.154 | 325 | 180 | 4 | 7 | 326 | 8 | 329 | 2.20e-84 | 258 |
| MS.gene87222.t1 | MTR_8g076820 | 45.033 | 302 | 163 | 3 | 27 | 326 | 30 | 330 | 3.31e-84 | 257 |
| MS.gene87222.t1 | MTR_4g114210 | 41.615 | 322 | 178 | 4 | 10 | 326 | 4 | 320 | 3.47e-83 | 254 |
| MS.gene87222.t1 | MTR_2g029730 | 41.896 | 327 | 186 | 3 | 3 | 326 | 1 | 326 | 7.53e-82 | 252 |
| MS.gene87222.t1 | MTR_2g029860 | 42.462 | 325 | 183 | 3 | 5 | 326 | 7 | 330 | 1.18e-81 | 251 |
| MS.gene87222.t1 | MTR_3g466200 | 42.216 | 334 | 171 | 8 | 1 | 326 | 1 | 320 | 1.27e-81 | 250 |
| MS.gene87222.t1 | MTR_3g072190 | 41.925 | 322 | 176 | 4 | 8 | 326 | 5 | 318 | 5.39e-81 | 249 |
| MS.gene87222.t1 | MTR_7g086820 | 41.776 | 304 | 166 | 4 | 25 | 326 | 18 | 312 | 6.10e-81 | 248 |
| MS.gene87222.t1 | MTR_2g084080 | 39.938 | 323 | 183 | 4 | 7 | 326 | 3 | 317 | 6.89e-80 | 246 |
| MS.gene87222.t1 | MTR_1g025980 | 41.009 | 317 | 178 | 4 | 11 | 325 | 8 | 317 | 7.26e-80 | 246 |
| MS.gene87222.t1 | MTR_2g084110 | 39.450 | 327 | 187 | 4 | 1 | 326 | 1 | 317 | 1.24e-79 | 245 |
| MS.gene87222.t1 | MTR_6g027440 | 40.498 | 321 | 179 | 5 | 10 | 326 | 2 | 314 | 1.27e-79 | 245 |
| MS.gene87222.t1 | MTR_5g074970 | 40.625 | 320 | 177 | 5 | 12 | 326 | 15 | 326 | 1.67e-79 | 245 |
| MS.gene87222.t1 | MTR_2g029910 | 42.547 | 322 | 176 | 6 | 13 | 326 | 12 | 332 | 5.15e-79 | 245 |
| MS.gene87222.t1 | MTR_1g086320 | 40.498 | 321 | 185 | 4 | 11 | 326 | 14 | 333 | 5.52e-79 | 244 |
| MS.gene87222.t1 | MTR_7g072510 | 38.365 | 318 | 187 | 3 | 10 | 326 | 3 | 312 | 7.06e-79 | 243 |
| MS.gene87222.t1 | MTR_8g062780 | 39.432 | 317 | 187 | 4 | 13 | 326 | 10 | 324 | 8.68e-79 | 243 |
| MS.gene87222.t1 | MTR_7g029030 | 41.772 | 316 | 176 | 4 | 13 | 326 | 11 | 320 | 2.32e-78 | 242 |
| MS.gene87222.t1 | MTR_1g086490 | 38.415 | 328 | 189 | 4 | 11 | 326 | 14 | 340 | 1.30e-77 | 241 |
| MS.gene87222.t1 | MTR_4g029170 | 39.818 | 329 | 185 | 6 | 3 | 326 | 1 | 321 | 1.59e-77 | 240 |
| MS.gene87222.t1 | MTR_4g125940 | 45.091 | 275 | 143 | 4 | 57 | 326 | 3 | 274 | 1.83e-77 | 238 |
| MS.gene87222.t1 | MTR_7g072480 | 39.937 | 318 | 182 | 3 | 10 | 326 | 7 | 316 | 1.91e-77 | 239 |
| MS.gene87222.t1 | MTR_2g437770 | 45.215 | 303 | 152 | 7 | 27 | 326 | 50 | 341 | 1.94e-77 | 240 |
| MS.gene87222.t1 | MTR_4g087965 | 42.434 | 304 | 168 | 5 | 26 | 323 | 41 | 343 | 6.45e-77 | 239 |
| MS.gene87222.t1 | MTR_4g083710 | 39.264 | 326 | 183 | 6 | 9 | 326 | 6 | 324 | 6.59e-77 | 238 |
| MS.gene87222.t1 | MTR_2g084020 | 41.694 | 307 | 169 | 4 | 22 | 326 | 20 | 318 | 1.19e-76 | 238 |
| MS.gene87222.t1 | MTR_1g115900 | 40.625 | 320 | 175 | 7 | 11 | 326 | 13 | 321 | 1.27e-76 | 238 |
| MS.gene87222.t1 | MTR_7g086870 | 40.066 | 302 | 171 | 4 | 25 | 326 | 20 | 311 | 2.40e-76 | 237 |
| MS.gene87222.t1 | MTR_3g094670 | 40.635 | 315 | 176 | 4 | 11 | 322 | 12 | 318 | 2.40e-76 | 237 |
| MS.gene87222.t1 | MTR_4g029190 | 38.209 | 335 | 180 | 5 | 12 | 326 | 13 | 340 | 1.34e-75 | 236 |
| MS.gene87222.t1 | MTR_5g083860 | 40.187 | 321 | 179 | 4 | 12 | 326 | 12 | 325 | 1.53e-75 | 235 |
| MS.gene87222.t1 | MTR_4g029200 | 37.126 | 334 | 183 | 4 | 12 | 326 | 13 | 338 | 1.88e-75 | 235 |
| MS.gene87222.t1 | MTR_2g084090 | 39.450 | 327 | 188 | 3 | 1 | 326 | 1 | 318 | 1.91e-75 | 234 |
| MS.gene87222.t1 | MTR_5g049280 | 40.385 | 312 | 177 | 7 | 22 | 326 | 28 | 337 | 2.63e-75 | 235 |
| MS.gene87222.t1 | MTR_2g084010 | 41.694 | 307 | 169 | 4 | 22 | 326 | 20 | 318 | 3.25e-75 | 234 |
| MS.gene87222.t1 | MTR_5g016010 | 40.439 | 319 | 185 | 2 | 11 | 325 | 11 | 328 | 9.47e-75 | 233 |
| MS.gene87222.t1 | MTR_5g074740 | 40.606 | 330 | 181 | 8 | 3 | 326 | 1 | 321 | 1.16e-74 | 233 |
| MS.gene87222.t1 | MTR_4g030890 | 39.935 | 308 | 180 | 4 | 20 | 326 | 93 | 396 | 2.01e-74 | 235 |
| MS.gene87222.t1 | MTR_5g074760 | 40.303 | 330 | 182 | 8 | 3 | 326 | 1 | 321 | 2.07e-74 | 232 |
| MS.gene87222.t1 | MTR_5g014100 | 43.046 | 302 | 166 | 4 | 31 | 326 | 30 | 331 | 4.64e-74 | 231 |
| MS.gene87222.t1 | MTR_4g122640 | 41.042 | 307 | 174 | 5 | 23 | 326 | 34 | 336 | 6.63e-74 | 231 |
| MS.gene87222.t1 | MTR_1g022970 | 42.244 | 303 | 171 | 3 | 27 | 326 | 46 | 347 | 1.28e-73 | 231 |
| MS.gene87222.t1 | MTR_4g029180 | 40.468 | 299 | 167 | 5 | 31 | 326 | 31 | 321 | 1.49e-73 | 230 |
| MS.gene87222.t1 | MTR_4g074995 | 39.375 | 320 | 169 | 6 | 12 | 326 | 9 | 308 | 1.14e-72 | 227 |
| MS.gene87222.t1 | MTR_4g031140 | 38.689 | 305 | 182 | 4 | 23 | 326 | 24 | 324 | 1.66e-72 | 228 |
| MS.gene87222.t1 | MTR_4g133800 | 41.946 | 298 | 164 | 5 | 30 | 326 | 44 | 333 | 1.93e-72 | 228 |
| MS.gene87222.t1 | MTR_4g095450 | 40.566 | 318 | 177 | 4 | 12 | 325 | 14 | 323 | 1.96e-72 | 227 |
| MS.gene87222.t1 | MTR_1g090760 | 37.771 | 323 | 195 | 5 | 9 | 326 | 9 | 330 | 2.00e-72 | 227 |
| MS.gene87222.t1 | MTR_1g009750 | 41.515 | 330 | 185 | 5 | 3 | 326 | 1 | 328 | 2.94e-72 | 227 |
| MS.gene87222.t1 | MTR_3g094630 | 40.000 | 315 | 178 | 4 | 11 | 322 | 12 | 318 | 6.95e-72 | 226 |
| MS.gene87222.t1 | MTR_2g084000 | 38.006 | 321 | 189 | 4 | 8 | 326 | 7 | 319 | 8.88e-72 | 225 |
| MS.gene87222.t1 | MTR_2g008730 | 40.367 | 327 | 159 | 6 | 7 | 326 | 4 | 301 | 7.47e-70 | 219 |
| MS.gene87222.t1 | MTR_4g127670 | 40.134 | 299 | 175 | 3 | 26 | 322 | 23 | 319 | 1.16e-69 | 220 |
| MS.gene87222.t1 | MTR_2g008160 | 40.385 | 312 | 176 | 5 | 20 | 326 | 17 | 323 | 2.19e-69 | 219 |
| MS.gene87222.t1 | MTR_2g029830 | 45.349 | 258 | 137 | 3 | 72 | 326 | 5 | 261 | 2.91e-69 | 218 |
| MS.gene87222.t1 | MTR_7g026990 | 38.730 | 315 | 183 | 4 | 13 | 326 | 17 | 322 | 4.92e-69 | 218 |
| MS.gene87222.t1 | MTR_3g467600 | 40.729 | 329 | 176 | 9 | 6 | 326 | 7 | 324 | 7.55e-69 | 218 |
| MS.gene87222.t1 | MTR_5g074710 | 37.879 | 330 | 190 | 8 | 3 | 326 | 1 | 321 | 2.35e-68 | 216 |
| MS.gene87222.t1 | MTR_3g466180 | 38.291 | 316 | 186 | 3 | 12 | 326 | 16 | 323 | 3.82e-68 | 216 |
| MS.gene87222.t1 | MTR_4g074990 | 37.658 | 316 | 177 | 5 | 12 | 326 | 9 | 305 | 3.93e-67 | 213 |
| MS.gene87222.t1 | MTR_3g105790 | 38.554 | 332 | 181 | 6 | 5 | 326 | 4 | 322 | 6.65e-66 | 210 |
| MS.gene87222.t1 | MTR_6g043460 | 35.045 | 331 | 197 | 6 | 1 | 322 | 3 | 324 | 1.04e-65 | 211 |
| MS.gene87222.t1 | MTR_3g072190 | 43.191 | 257 | 137 | 3 | 71 | 326 | 3 | 251 | 1.14e-65 | 207 |
| MS.gene87222.t1 | MTR_2g099175 | 38.344 | 326 | 188 | 6 | 9 | 326 | 15 | 335 | 6.23e-65 | 208 |
| MS.gene87222.t1 | MTR_1g009750 | 39.264 | 326 | 181 | 6 | 3 | 326 | 1 | 311 | 1.08e-63 | 204 |
| MS.gene87222.t1 | MTR_5g074860 | 36.923 | 325 | 186 | 8 | 6 | 322 | 8 | 321 | 1.57e-62 | 202 |
| MS.gene87222.t1 | MTR_2g067440 | 35.866 | 329 | 179 | 5 | 25 | 323 | 58 | 384 | 3.23e-62 | 202 |
| MS.gene87222.t1 | MTR_6g043240 | 36.826 | 334 | 185 | 7 | 7 | 326 | 10 | 331 | 5.74e-62 | 201 |
| MS.gene87222.t1 | MTR_0286s0010 | 36.250 | 320 | 195 | 6 | 12 | 326 | 19 | 334 | 8.26e-62 | 200 |
| MS.gene87222.t1 | MTR_6g008075 | 36.250 | 320 | 195 | 6 | 12 | 326 | 19 | 334 | 8.26e-62 | 200 |
| MS.gene87222.t1 | MTR_4g132110 | 36.455 | 299 | 183 | 2 | 30 | 326 | 35 | 328 | 1.21e-61 | 199 |
| MS.gene87222.t1 | MTR_6g043280 | 35.922 | 309 | 182 | 7 | 24 | 326 | 34 | 332 | 2.84e-61 | 199 |
| MS.gene87222.t1 | MTR_2g078610 | 36.937 | 333 | 173 | 7 | 25 | 323 | 74 | 403 | 8.16e-61 | 199 |
| MS.gene87222.t1 | MTR_2g077990 | 36.858 | 331 | 176 | 5 | 25 | 323 | 74 | 403 | 1.08e-60 | 199 |
| MS.gene87222.t1 | MTR_4g132110 | 35.855 | 304 | 183 | 3 | 30 | 326 | 35 | 333 | 3.38e-59 | 193 |
| MS.gene87222.t1 | MTR_1g066710 | 35.738 | 305 | 187 | 3 | 25 | 326 | 25 | 323 | 3.52e-59 | 193 |
| MS.gene87222.t1 | MTR_4g074980 | 36.562 | 320 | 164 | 6 | 11 | 326 | 10 | 294 | 1.36e-58 | 191 |
| MS.gene87222.t1 | MTR_4g074980 | 42.063 | 252 | 134 | 5 | 11 | 258 | 10 | 253 | 3.50e-58 | 188 |
| MS.gene87222.t1 | MTR_1g066380 | 35.599 | 309 | 182 | 6 | 25 | 326 | 25 | 323 | 1.33e-57 | 189 |
| MS.gene87222.t1 | MTR_1g066640 | 34.754 | 305 | 190 | 3 | 25 | 326 | 25 | 323 | 4.48e-57 | 187 |
| MS.gene87222.t1 | MTR_2g078280 | 37.107 | 318 | 167 | 5 | 25 | 310 | 74 | 390 | 1.14e-56 | 188 |
| MS.gene87222.t1 | MTR_1g066680 | 35.082 | 305 | 188 | 4 | 25 | 326 | 25 | 322 | 2.37e-56 | 186 |
| MS.gene87222.t1 | MTR_4g132490 | 33.645 | 321 | 202 | 4 | 11 | 326 | 13 | 327 | 1.73e-55 | 183 |
| MS.gene87222.t1 | MTR_2g008160 | 36.218 | 312 | 154 | 5 | 20 | 326 | 17 | 288 | 3.40e-55 | 181 |
| MS.gene87222.t1 | MTR_2g067450 | 37.255 | 306 | 182 | 6 | 25 | 325 | 9 | 309 | 3.40e-55 | 182 |
| MS.gene87222.t1 | MTR_5g033470 | 35.256 | 312 | 195 | 5 | 21 | 326 | 67 | 377 | 1.61e-54 | 182 |
| MS.gene87222.t1 | MTR_0147s0010 | 34.385 | 317 | 174 | 5 | 27 | 323 | 77 | 379 | 1.77e-53 | 180 |
| MS.gene87222.t1 | MTR_2g008160 | 46.111 | 180 | 94 | 2 | 20 | 199 | 17 | 193 | 1.88e-47 | 159 |
| MS.gene87222.t1 | MTR_3g105790 | 37.945 | 253 | 138 | 4 | 84 | 326 | 3 | 246 | 1.62e-45 | 155 |
| MS.gene87222.t1 | MTR_5g074860 | 31.889 | 323 | 166 | 7 | 6 | 322 | 8 | 282 | 3.55e-42 | 147 |
| MS.gene87222.t1 | MTR_1g066380 | 33.460 | 263 | 158 | 6 | 71 | 326 | 3 | 255 | 4.13e-42 | 147 |
| MS.gene87222.t1 | MTR_5g074700 | 36.910 | 233 | 123 | 8 | 97 | 326 | 3 | 214 | 3.17e-37 | 132 |
| MS.gene87222.t1 | MTR_2g029880 | 39.286 | 196 | 106 | 5 | 133 | 326 | 4 | 188 | 1.68e-35 | 128 |
| MS.gene87222.t1 | MTR_7g072490 | 33.871 | 248 | 101 | 5 | 73 | 319 | 2 | 187 | 5.63e-32 | 119 |
| MS.gene87222.t1 | MTR_3g072210 | 35.751 | 193 | 114 | 4 | 136 | 326 | 54 | 238 | 4.80e-31 | 117 |
| MS.gene87222.t1 | MTR_3g072260 | 45.000 | 140 | 72 | 3 | 8 | 144 | 5 | 142 | 2.19e-29 | 110 |
| MS.gene87222.t1 | MTR_1g089005 | 45.669 | 127 | 56 | 2 | 12 | 132 | 7 | 126 | 5.84e-29 | 109 |
| MS.gene87222.t1 | MTR_7g086790 | 33.503 | 197 | 105 | 5 | 74 | 268 | 17 | 189 | 4.34e-24 | 97.8 |
| MS.gene87222.t1 | MTR_2g077980 | 28.622 | 283 | 162 | 8 | 79 | 323 | 1 | 281 | 1.99e-23 | 98.2 |
| MS.gene87222.t1 | MTR_6g048160 | 61.765 | 68 | 23 | 1 | 66 | 133 | 22 | 86 | 1.35e-22 | 90.5 |
| MS.gene87222.t1 | MTR_7g086810 | 42.975 | 121 | 44 | 1 | 25 | 145 | 4 | 99 | 3.85e-21 | 90.9 |
| MS.gene87222.t1 | MTR_4g114220 | 50.000 | 80 | 31 | 2 | 55 | 134 | 31 | 101 | 1.73e-20 | 86.3 |
| MS.gene87222.t1 | MTR_5g058120 | 34.969 | 163 | 92 | 4 | 161 | 322 | 6 | 155 | 4.11e-20 | 88.2 |
| MS.gene87222.t1 | MTR_2g078560 | 25.166 | 302 | 177 | 6 | 71 | 325 | 20 | 319 | 8.26e-20 | 88.6 |
| MS.gene87222.t1 | MTR_7g107500 | 46.341 | 82 | 43 | 1 | 245 | 326 | 17 | 97 | 7.35e-19 | 80.5 |
| MS.gene87222.t1 | MTR_4g012865 | 50.000 | 68 | 34 | 0 | 27 | 94 | 77 | 144 | 1.47e-15 | 73.2 |
| MS.gene87222.t1 | MTR_6g071110 | 45.745 | 94 | 39 | 3 | 93 | 186 | 80 | 161 | 5.09e-15 | 72.0 |
| MS.gene87222.t1 | MTR_7g086785 | 35.897 | 117 | 68 | 2 | 72 | 181 | 3 | 119 | 8.77e-15 | 70.9 |
| MS.gene87222.t1 | MTR_2g029930 | 33.333 | 108 | 42 | 4 | 13 | 104 | 1 | 94 | 5.93e-12 | 62.0 |
| MS.gene87222.t1 | MTR_4g031040 | 45.000 | 60 | 33 | 0 | 137 | 196 | 26 | 85 | 5.50e-11 | 60.1 |
| MS.gene87222.t1 | MTR_1g098320 | 39.744 | 78 | 47 | 0 | 249 | 326 | 47 | 124 | 7.51e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87222.t1 | AT4G11290 | 61.371 | 321 | 116 | 3 | 11 | 326 | 9 | 326 | 3.72e-146 | 415 |
| MS.gene87222.t1 | AT1G05260 | 62.179 | 312 | 116 | 2 | 16 | 326 | 15 | 325 | 1.28e-145 | 413 |
| MS.gene87222.t1 | AT3G21770 | 59.223 | 309 | 124 | 2 | 16 | 323 | 18 | 325 | 3.79e-135 | 387 |
| MS.gene87222.t1 | AT5G15180 | 53.750 | 320 | 143 | 5 | 9 | 326 | 13 | 329 | 3.34e-120 | 349 |
| MS.gene87222.t1 | AT3G01190 | 51.840 | 326 | 150 | 6 | 3 | 326 | 1 | 321 | 6.01e-114 | 333 |
| MS.gene87222.t1 | AT1G05240 | 49.669 | 302 | 140 | 5 | 31 | 326 | 30 | 325 | 3.13e-103 | 306 |
| MS.gene87222.t1 | AT1G05250 | 49.669 | 302 | 140 | 5 | 31 | 326 | 30 | 325 | 3.13e-103 | 306 |
| MS.gene87222.t1 | AT2G18150 | 44.156 | 308 | 169 | 3 | 21 | 326 | 30 | 336 | 1.49e-90 | 274 |
| MS.gene87222.t1 | AT2G39040 | 46.965 | 313 | 157 | 7 | 21 | 326 | 40 | 350 | 4.88e-88 | 268 |
| MS.gene87222.t1 | AT5G51890 | 42.320 | 319 | 173 | 5 | 12 | 326 | 11 | 322 | 2.08e-87 | 265 |
| MS.gene87222.t1 | AT2G18140 | 43.077 | 325 | 181 | 4 | 5 | 326 | 12 | 335 | 2.70e-86 | 263 |
| MS.gene87222.t1 | AT5G19890 | 43.161 | 329 | 173 | 7 | 3 | 326 | 8 | 327 | 3.36e-86 | 262 |
| MS.gene87222.t1 | AT4G36430 | 42.138 | 318 | 178 | 4 | 14 | 326 | 14 | 330 | 5.00e-86 | 262 |
| MS.gene87222.t1 | AT2G41480 | 42.638 | 326 | 175 | 6 | 8 | 326 | 8 | 328 | 1.81e-85 | 261 |
| MS.gene87222.t1 | AT5G14130 | 41.824 | 318 | 178 | 5 | 12 | 326 | 17 | 330 | 2.55e-85 | 260 |
| MS.gene87222.t1 | AT4G33420 | 42.902 | 317 | 168 | 5 | 12 | 326 | 20 | 325 | 2.86e-85 | 260 |
| MS.gene87222.t1 | AT5G17820 | 42.357 | 314 | 171 | 4 | 13 | 326 | 10 | 313 | 3.68e-85 | 259 |
| MS.gene87222.t1 | AT3G03670 | 40.881 | 318 | 186 | 2 | 9 | 326 | 6 | 321 | 4.79e-85 | 259 |
| MS.gene87222.t1 | AT5G42180 | 42.444 | 311 | 174 | 3 | 12 | 322 | 8 | 313 | 6.88e-85 | 259 |
| MS.gene87222.t1 | AT5G14130 | 41.824 | 318 | 178 | 5 | 12 | 326 | 58 | 371 | 8.54e-85 | 260 |
| MS.gene87222.t1 | AT3G32980 | 42.587 | 317 | 176 | 4 | 15 | 326 | 19 | 334 | 8.72e-85 | 259 |
| MS.gene87222.t1 | AT5G64120 | 41.317 | 334 | 180 | 6 | 3 | 326 | 1 | 328 | 1.51e-84 | 258 |
| MS.gene87222.t1 | AT5G39580 | 42.378 | 328 | 176 | 7 | 3 | 326 | 1 | 319 | 1.71e-84 | 258 |
| MS.gene87222.t1 | AT3G50990 | 45.928 | 307 | 163 | 3 | 22 | 326 | 36 | 341 | 2.37e-84 | 258 |
| MS.gene87222.t1 | AT4G33420 | 42.902 | 317 | 168 | 5 | 12 | 326 | 67 | 372 | 2.65e-84 | 259 |
| MS.gene87222.t1 | AT2G38390 | 40.625 | 320 | 185 | 4 | 11 | 326 | 15 | 333 | 3.63e-84 | 258 |
| MS.gene87222.t1 | AT5G17820 | 42.357 | 314 | 171 | 4 | 13 | 326 | 63 | 366 | 3.98e-84 | 258 |
| MS.gene87222.t1 | AT5G06720 | 40.252 | 318 | 186 | 3 | 12 | 326 | 17 | 333 | 1.87e-83 | 256 |
| MS.gene87222.t1 | AT2G38380 | 41.250 | 320 | 183 | 4 | 11 | 326 | 15 | 333 | 1.90e-83 | 256 |
| MS.gene87222.t1 | AT4G08770 | 43.269 | 312 | 165 | 6 | 23 | 326 | 20 | 327 | 3.19e-83 | 255 |
| MS.gene87222.t1 | AT5G66390 | 43.175 | 315 | 176 | 3 | 14 | 326 | 20 | 333 | 4.96e-83 | 254 |
| MS.gene87222.t1 | AT3G49110 | 41.846 | 325 | 181 | 5 | 9 | 326 | 13 | 336 | 7.24e-82 | 252 |
| MS.gene87222.t1 | AT3G49120 | 41.640 | 317 | 179 | 4 | 15 | 326 | 20 | 335 | 2.12e-81 | 251 |
| MS.gene87222.t1 | AT5G06730 | 38.994 | 318 | 190 | 4 | 12 | 326 | 18 | 334 | 1.00e-80 | 249 |
| MS.gene87222.t1 | AT2G24800 | 41.967 | 305 | 170 | 3 | 25 | 326 | 29 | 329 | 1.47e-80 | 248 |
| MS.gene87222.t1 | AT1G14550 | 41.433 | 321 | 178 | 5 | 9 | 326 | 8 | 321 | 1.48e-79 | 245 |
| MS.gene87222.t1 | AT5G64100 | 43.333 | 300 | 159 | 5 | 30 | 326 | 40 | 331 | 2.66e-79 | 245 |
| MS.gene87222.t1 | AT4G37530 | 42.122 | 311 | 175 | 2 | 20 | 326 | 20 | 329 | 1.09e-78 | 243 |
| MS.gene87222.t1 | AT1G71695 | 43.234 | 303 | 162 | 5 | 27 | 326 | 44 | 339 | 9.04e-78 | 242 |
| MS.gene87222.t1 | AT4G08780 | 41.987 | 312 | 169 | 6 | 23 | 326 | 20 | 327 | 1.09e-77 | 241 |
| MS.gene87222.t1 | AT1G44970 | 42.122 | 311 | 176 | 3 | 19 | 326 | 37 | 346 | 2.17e-77 | 241 |
| MS.gene87222.t1 | AT2G18980 | 42.553 | 329 | 178 | 5 | 3 | 326 | 1 | 323 | 3.66e-77 | 239 |
| MS.gene87222.t1 | AT1G49570 | 41.447 | 304 | 173 | 4 | 25 | 324 | 46 | 348 | 1.60e-76 | 238 |
| MS.gene87222.t1 | AT4G31760 | 40.252 | 318 | 181 | 5 | 12 | 326 | 15 | 326 | 2.41e-76 | 237 |
| MS.gene87222.t1 | AT1G14540 | 39.375 | 320 | 184 | 5 | 9 | 326 | 4 | 315 | 3.78e-75 | 234 |
| MS.gene87222.t1 | AT5G05340 | 40.656 | 305 | 172 | 3 | 24 | 326 | 27 | 324 | 4.50e-75 | 234 |
| MS.gene87222.t1 | AT5G19880 | 39.091 | 330 | 194 | 5 | 3 | 326 | 1 | 329 | 5.06e-75 | 234 |
| MS.gene87222.t1 | AT4G16270 | 42.000 | 300 | 167 | 6 | 30 | 326 | 53 | 348 | 2.70e-74 | 233 |
| MS.gene87222.t1 | AT4G37520 | 40.850 | 306 | 176 | 2 | 25 | 326 | 25 | 329 | 3.99e-74 | 231 |
| MS.gene87222.t1 | AT4G26010 | 39.557 | 316 | 175 | 6 | 12 | 326 | 9 | 309 | 4.36e-74 | 231 |
| MS.gene87222.t1 | AT4G26010 | 39.557 | 316 | 175 | 6 | 12 | 326 | 37 | 337 | 9.04e-74 | 231 |
| MS.gene87222.t1 | AT4G25980 | 39.252 | 321 | 185 | 5 | 12 | 326 | 10 | 326 | 1.24e-73 | 230 |
| MS.gene87222.t1 | AT1G77100 | 41.346 | 312 | 169 | 6 | 20 | 326 | 17 | 319 | 2.74e-73 | 229 |
| MS.gene87222.t1 | AT5G58400 | 40.673 | 327 | 183 | 6 | 1 | 322 | 1 | 321 | 4.03e-73 | 229 |
| MS.gene87222.t1 | AT5G64110 | 40.000 | 300 | 171 | 4 | 30 | 326 | 37 | 330 | 1.15e-72 | 228 |
| MS.gene87222.t1 | AT2G35380 | 38.776 | 343 | 185 | 6 | 1 | 326 | 1 | 335 | 1.64e-72 | 228 |
| MS.gene87222.t1 | AT2G22420 | 37.539 | 317 | 192 | 5 | 11 | 326 | 8 | 319 | 2.04e-72 | 227 |
| MS.gene87222.t1 | AT3G49960 | 40.741 | 324 | 183 | 4 | 11 | 326 | 7 | 329 | 2.73e-72 | 227 |
| MS.gene87222.t1 | AT4G37520 | 40.850 | 306 | 173 | 3 | 25 | 326 | 25 | 326 | 9.74e-72 | 225 |
| MS.gene87222.t1 | AT5G67400 | 40.453 | 309 | 179 | 2 | 22 | 326 | 22 | 329 | 5.86e-71 | 223 |
| MS.gene87222.t1 | AT5G22410 | 40.379 | 317 | 177 | 7 | 11 | 322 | 12 | 321 | 4.24e-70 | 221 |
| MS.gene87222.t1 | AT1G68850 | 38.411 | 302 | 180 | 4 | 31 | 326 | 34 | 335 | 6.02e-70 | 221 |
| MS.gene87222.t1 | AT4G33870 | 36.280 | 328 | 173 | 3 | 31 | 323 | 72 | 398 | 6.12e-70 | 223 |
| MS.gene87222.t1 | AT4G17690 | 38.141 | 312 | 184 | 4 | 18 | 326 | 17 | 322 | 6.22e-70 | 221 |
| MS.gene87222.t1 | AT5G58390 | 39.252 | 321 | 178 | 6 | 9 | 322 | 2 | 312 | 7.78e-70 | 220 |
| MS.gene87222.t1 | AT1G34510 | 38.291 | 316 | 179 | 6 | 12 | 326 | 9 | 309 | 2.23e-69 | 219 |
| MS.gene87222.t1 | AT2G34060 | 39.145 | 304 | 180 | 3 | 24 | 322 | 39 | 342 | 7.98e-69 | 218 |
| MS.gene87222.t1 | AT4G30170 | 39.542 | 306 | 176 | 3 | 25 | 326 | 25 | 325 | 3.81e-68 | 216 |
| MS.gene87222.t1 | AT4G37530 | 44.231 | 260 | 140 | 2 | 20 | 275 | 20 | 278 | 5.30e-68 | 214 |
| MS.gene87222.t1 | AT1G30870 | 41.806 | 299 | 163 | 6 | 31 | 326 | 55 | 345 | 1.66e-66 | 213 |
| MS.gene87222.t1 | AT4G21960 | 36.036 | 333 | 198 | 6 | 3 | 326 | 1 | 327 | 2.04e-66 | 212 |
| MS.gene87222.t1 | AT5G47000 | 37.538 | 325 | 186 | 7 | 11 | 326 | 14 | 330 | 9.58e-66 | 210 |
| MS.gene87222.t1 | AT5G40150 | 36.482 | 307 | 188 | 2 | 22 | 326 | 27 | 328 | 9.71e-64 | 205 |
| MS.gene87222.t1 | AT1G24110 | 35.443 | 316 | 194 | 4 | 14 | 322 | 6 | 318 | 2.47e-63 | 204 |
| MS.gene87222.t1 | AT2G43480 | 37.261 | 314 | 186 | 4 | 19 | 326 | 27 | 335 | 6.31e-62 | 200 |
| MS.gene87222.t1 | AT5G24070 | 35.312 | 337 | 195 | 6 | 7 | 326 | 5 | 335 | 1.97e-61 | 199 |
| MS.gene87222.t1 | AT3G28200 | 35.505 | 307 | 190 | 2 | 22 | 326 | 16 | 316 | 2.83e-61 | 198 |
| MS.gene87222.t1 | AT2G37130 | 33.876 | 307 | 193 | 4 | 24 | 326 | 27 | 327 | 2.46e-54 | 181 |
| MS.gene87222.t1 | AT2G37130 | 34.219 | 301 | 188 | 4 | 30 | 326 | 2 | 296 | 1.07e-53 | 178 |
| MS.gene87222.t1 | AT3G17070 | 32.440 | 336 | 203 | 7 | 2 | 322 | 9 | 335 | 6.41e-53 | 177 |
| MS.gene87222.t1 | AT2G35380 | 38.340 | 253 | 134 | 5 | 88 | 326 | 3 | 247 | 1.18e-48 | 163 |
| MS.gene87222.t1 | AT5G39580 | 44.279 | 201 | 102 | 4 | 3 | 200 | 1 | 194 | 8.30e-48 | 161 |
| MS.gene87222.t1 | AT4G25980 | 45.665 | 173 | 91 | 2 | 21 | 193 | 65 | 234 | 2.22e-45 | 156 |
| MS.gene87222.t1 | AT2G24800 | 45.509 | 167 | 88 | 1 | 25 | 191 | 29 | 192 | 7.20e-43 | 148 |
| MS.gene87222.t1 | AT3G17070 | 30.824 | 279 | 172 | 7 | 55 | 322 | 4 | 272 | 9.09e-39 | 139 |
| MS.gene87222.t1 | AT3G42570 | 33.019 | 106 | 70 | 1 | 89 | 193 | 44 | 149 | 1.20e-13 | 68.2 |
Find 62 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGACCTAGTCTTGCTTTC+TGG | 0.177027 | 4.4:+54104184 | MS.gene87222:CDS |
| ATTTGTCTTATAGCAATAAT+TGG | 0.198586 | 4.4:+54102949 | MS.gene87222:CDS |
| ATTTGCAGGGTGGGCCTTAT+TGG | 0.254925 | 4.4:+54104035 | MS.gene87222:intron |
| AAAACAATTTGTTCAGCTTT+GGG | 0.265521 | 4.4:-54103018 | None:intergenic |
| CAAAACAATTTGTTCAGCTT+TGG | 0.265636 | 4.4:-54103019 | None:intergenic |
| CTCTGCCAATTACATGGAAA+TGG | 0.326053 | 4.4:+54102897 | None:intergenic |
| GCATATTCATTGTCTAGATC+TGG | 0.329811 | 4.4:-54104661 | None:intergenic |
| TAGCCTCGAAGTGTAAGATT+TGG | 0.347680 | 4.4:-54103444 | None:intergenic |
| TCAAATGTATTGCGACTTCC+AGG | 0.350416 | 4.4:-54104748 | None:intergenic |
| GGAAGGTTCCAACAGGTAGA+AGG | 0.383934 | 4.4:+54104056 | MS.gene87222:CDS |
| TCAAACCACACTTATTGAAA+TGG | 0.390578 | 4.4:+54104723 | MS.gene87222:CDS |
| GGACGAATTAATGTCAAGAT+TGG | 0.394960 | 4.4:+54104925 | MS.gene87222:CDS |
| ACTGTCCTCCTAACACTCTT+TGG | 0.396163 | 4.4:+54104142 | MS.gene87222:CDS |
| CAACATTGCCAAAGAGTGTT+AGG | 0.396433 | 4.4:-54104150 | None:intergenic |
| CTTTCAGGTGCGCACACAAT+TGG | 0.397251 | 4.4:+54104574 | MS.gene87222:intron |
| TAAGCAATGCAGCATCTGAT+TGG | 0.403489 | 4.4:-54104813 | None:intergenic |
| AGTCGCAATACATTTGATCT+TGG | 0.429977 | 4.4:+54104754 | MS.gene87222:CDS |
| CTAACACTCTTTGGCAATGT+TGG | 0.442479 | 4.4:+54104151 | MS.gene87222:CDS |
| GCCAGTAAAATTATATAGCC+TGG | 0.446340 | 4.4:-54104623 | None:intergenic |
| ACAAACCAATCAAGCTGAAA+AGG | 0.448336 | 4.4:+54103413 | MS.gene87222:CDS |
| CCTGTTGGAACCTTCCAATA+AGG | 0.463341 | 4.4:-54104049 | None:intergenic |
| ATTCCAAATCTTACACTTCG+AGG | 0.467504 | 4.4:+54103441 | MS.gene87222:CDS |
| CATATTCATTGTCTAGATCT+GGG | 0.471482 | 4.4:-54104660 | None:intergenic |
| GGGAAGCCAGAGTTACTTCA+AGG | 0.476148 | 4.4:+54102918 | MS.gene87222:CDS |
| TCTGCCAATTACATGGAAAT+GGG | 0.479431 | 4.4:+54102898 | None:intergenic |
| TTAAAACCTTGAAGTAACTC+TGG | 0.484915 | 4.4:-54102924 | None:intergenic |
| GCAGGGTGGGCCTTATTGGA+AGG | 0.485522 | 4.4:+54104039 | MS.gene87222:intron |
| TACTGAATTTGGAAGGTCCA+TGG | 0.486526 | 4.4:+54104894 | MS.gene87222:CDS |
| GACGAATTAATGTCAAGATT+GGG | 0.493077 | 4.4:+54104926 | MS.gene87222:CDS |
| CCTTATTGGAAGGTTCCAAC+AGG | 0.506222 | 4.4:+54104049 | MS.gene87222:CDS |
| AGGACAGTGAGGTTGTGAAA+TGG | 0.507765 | 4.4:-54104127 | None:intergenic |
| CAAGCAAGTAGTTAAGAGAA+GGG | 0.509407 | 4.4:+54104783 | MS.gene87222:CDS |
| ATACCTTGACAAAACAATCA+TGG | 0.512809 | 4.4:-54103107 | None:intergenic |
| AATTCTTTACTGAATTTGGA+AGG | 0.512900 | 4.4:+54104887 | MS.gene87222:CDS |
| ACAAGCAAGTAGTTAAGAGA+AGG | 0.514948 | 4.4:+54104782 | MS.gene87222:CDS |
| AGCTGCTAGTGATGGTACAC+TGG | 0.519920 | 4.4:-54103064 | None:intergenic |
| GGTTGAAATTGATGAGCAAT+GGG | 0.524307 | 4.4:-54104602 | None:intergenic |
| ACTCCATCCCTTCTACCTGT+TGG | 0.547709 | 4.4:-54104064 | None:intergenic |
| GTGAGGTTGTGAAATGGAGC+AGG | 0.547960 | 4.4:-54104121 | None:intergenic |
| GAAGGTTCCAACAGGTAGAA+GGG | 0.566125 | 4.4:+54104057 | MS.gene87222:CDS |
| ACTCATGCTCAATTGCAGCC+AGG | 0.571279 | 4.4:+54102976 | MS.gene87222:CDS |
| GTTCCAACAGGTAGAAGGGA+TGG | 0.572964 | 4.4:+54104061 | MS.gene87222:CDS |
| AATGTCAAGATTGGGACTGA+AGG | 0.574020 | 4.4:+54104934 | MS.gene87222:CDS |
| AGGGTCCATTTCAATAAGTG+TGG | 0.580715 | 4.4:-54104728 | None:intergenic |
| CAGGGCATTCTTTCTCAACA+AGG | 0.582275 | 4.4:-54103491 | None:intergenic |
| TGGTTGAAATTGATGAGCAA+TGG | 0.583434 | 4.4:-54104603 | None:intergenic |
| ACACTTATTGAAATGGACCC+TGG | 0.584876 | 4.4:+54104730 | MS.gene87222:CDS |
| AAGCAAGTAGTTAAGAGAAG+GGG | 0.586770 | 4.4:+54104784 | MS.gene87222:CDS |
| TCAGCACAAGAGACAACACC+AGG | 0.593653 | 4.4:-54103510 | None:intergenic |
| AAACAATTTGTTCAGCTTTG+GGG | 0.596812 | 4.4:-54103017 | None:intergenic |
| ATGAGTGCAGCTGCTAGTGA+TGG | 0.606593 | 4.4:-54103072 | None:intergenic |
| ATTTCAGGGATGTGATGCAT+CGG | 0.627600 | 4.4:+54103374 | MS.gene87222:intron |
| GCATACCAGAAAGCAAGACT+AGG | 0.628390 | 4.4:-54104189 | None:intergenic |
| TGCAAGAATAGATCTTGTTG+TGG | 0.635514 | 4.4:-54104842 | None:intergenic |
| CTTGTTGAGAAAGAATGCCC+TGG | 0.654658 | 4.4:+54103492 | MS.gene87222:CDS |
| GGTTGTGAAATGGAGCAGGA+AGG | 0.663275 | 4.4:-54104117 | None:intergenic |
| ATTGCTCATCAATTTCAACC+AGG | 0.665224 | 4.4:+54104605 | MS.gene87222:CDS |
| CATTGCCAAAGAGTGTTAGG+AGG | 0.665435 | 4.4:-54104147 | None:intergenic |
| CAAATGTATTGCGACTTCCA+GGG | 0.678271 | 4.4:-54104747 | None:intergenic |
| GCTGCTAGTGATGGTACACT+GGG | 0.680818 | 4.4:-54103063 | None:intergenic |
| AGAGTGTTAGGAGGACAGTG+AGG | 0.689613 | 4.4:-54104138 | None:intergenic |
| CAGCACAAGAGACAACACCA+GGG | 0.782900 | 4.4:-54103509 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATAAATTAATTGAG+TGG | + | chr4.4:54103224-54103243 | MS.gene87222:intron | 10.0% |
| !! | TAATTTAAGAAAATAAAACT+AGG | + | chr4.4:54104379-54104398 | MS.gene87222:intron | 10.0% |
| !!! | TTATTTTCTTAAATTAATAC+TGG | - | chr4.4:54104375-54104394 | None:intergenic | 10.0% |
| !! | ATTTAGTACGTATAATTATT+TGG | + | chr4.4:54103957-54103976 | MS.gene87222:intron | 15.0% |
| !! | TCAATTAGATAAATGTTATT+TGG | + | chr4.4:54103840-54103859 | MS.gene87222:intron | 15.0% |
| !!! | GAAAATATTTTAAAGTGTTT+TGG | - | chr4.4:54103663-54103682 | None:intergenic | 15.0% |
| !! | AAAAAAACTATCATTCTTTC+AGG | + | chr4.4:54104559-54104578 | MS.gene87222:intron | 20.0% |
| !! | ATTTGTCTTATAGCAATAAT+TGG | + | chr4.4:54102949-54102968 | MS.gene87222:CDS | 20.0% |
| !! | GAAAAATTCTTTACTGAATT+TGG | + | chr4.4:54104883-54104902 | MS.gene87222:CDS | 20.0% |
| !!! | AATATTTTAAAGTGTTTTGG+AGG | - | chr4.4:54103660-54103679 | None:intergenic | 20.0% |
| !!! | AGTCTAAATTTTTTCAGATA+GGG | + | chr4.4:54103737-54103756 | MS.gene87222:intron | 20.0% |
| !!! | ATAATTATTTGGTTTTCTAG+TGG | + | chr4.4:54103968-54103987 | MS.gene87222:intron | 20.0% |
| !!! | TAGTCTAAATTTTTTCAGAT+AGG | + | chr4.4:54103736-54103755 | MS.gene87222:intron | 20.0% |
| !!! | TTTAGTTTGTGTGTAAAATT+TGG | - | chr4.4:54104214-54104233 | None:intergenic | 20.0% |
| !!! | TTTTAGTATCTCATTTCTAT+AGG | + | chr4.4:54103596-54103615 | MS.gene87222:intron | 20.0% |
| ! | AATTCTTTACTGAATTTGGA+AGG | + | chr4.4:54104887-54104906 | MS.gene87222:CDS | 25.0% |
| ! | CAGATAGGGTATATATATTT+GGG | + | chr4.4:54103751-54103770 | MS.gene87222:intron | 25.0% |
| ! | CTAAATTCCATTATCATTAC+AGG | + | chr4.4:54104229-54104248 | MS.gene87222:intron | 25.0% |
| ! | TCAGATAGGGTATATATATT+TGG | + | chr4.4:54103750-54103769 | MS.gene87222:intron | 25.0% |
| !! | AAAACAATTTGTTCAGCTTT+GGG | - | chr4.4:54103021-54103040 | None:intergenic | 25.0% |
| !! | AAATGTTATTTGGTTATGCA+TGG | + | chr4.4:54103850-54103869 | MS.gene87222:intron | 25.0% |
| !! | CTATATAATTTTACTGGCAA+GGG | + | chr4.4:54104628-54104647 | MS.gene87222:CDS | 25.0% |
| !! | GGTTTTTATAAGATGAGAAA+AGG | - | chr4.4:54103639-54103658 | None:intergenic | 25.0% |
| !!! | TCGATTTTTTAATCTGAGTA+CGG | + | chr4.4:54103681-54103700 | MS.gene87222:intron | 25.0% |
| ATACCTTGACAAAACAATCA+TGG | - | chr4.4:54103110-54103129 | None:intergenic | 30.0% | |
| CATATTCATTGTCTAGATCT+GGG | - | chr4.4:54104663-54104682 | None:intergenic | 30.0% | |
| GACGAATTAATGTCAAGATT+GGG | + | chr4.4:54104926-54104945 | MS.gene87222:CDS | 30.0% | |
| TAGGTTAATGTAACTACATG+TGG | + | chr4.4:54104398-54104417 | MS.gene87222:intron | 30.0% | |
| TCAAACCACACTTATTGAAA+TGG | + | chr4.4:54104723-54104742 | MS.gene87222:CDS | 30.0% | |
| TGCCTAAAAAAACATTGACA+CGG | + | chr4.4:54104314-54104333 | MS.gene87222:intron | 30.0% | |
| TTAAAACCTTGAAGTAACTC+TGG | - | chr4.4:54102927-54102946 | None:intergenic | 30.0% | |
| ! | AAACAATTTGTTCAGCTTTG+GGG | - | chr4.4:54103020-54103039 | None:intergenic | 30.0% |
| ! | ACCAGGCTATATAATTTTAC+TGG | + | chr4.4:54104622-54104641 | MS.gene87222:CDS | 30.0% |
| ! | CAAAACAATTTGTTCAGCTT+TGG | - | chr4.4:54103022-54103041 | None:intergenic | 30.0% |
| ! | GCTATATAATTTTACTGGCA+AGG | + | chr4.4:54104627-54104646 | MS.gene87222:CDS | 30.0% |
| !! | TTTCCATGATTGTTTTGTCA+AGG | + | chr4.4:54103104-54103123 | MS.gene87222:CDS | 30.0% |
| !!! | ATATTTTGACTTTGACTGCT+AGG | + | chr4.4:54103532-54103551 | MS.gene87222:CDS | 30.0% |
| !!! | TATTTTGACTTTGACTGCTA+GGG | + | chr4.4:54103533-54103552 | MS.gene87222:CDS | 30.0% |
| AACTAACATCTGGCATTTCA+GGG | + | chr4.4:54103360-54103379 | MS.gene87222:intron | 35.0% | |
| AAGCAAGTAGTTAAGAGAAG+GGG | + | chr4.4:54104784-54104803 | MS.gene87222:CDS | 35.0% | |
| ACAAACCAATCAAGCTGAAA+AGG | + | chr4.4:54103413-54103432 | MS.gene87222:CDS | 35.0% | |
| ACAAGCAAGTAGTTAAGAGA+AGG | + | chr4.4:54104782-54104801 | MS.gene87222:CDS | 35.0% | |
| ACGTTGAACATGTCTTCTAT+CGG | + | chr4.4:54104497-54104516 | MS.gene87222:intron | 35.0% | |
| AGTCGCAATACATTTGATCT+TGG | + | chr4.4:54104754-54104773 | MS.gene87222:CDS | 35.0% | |
| ATCATGGCAGAACAAATAAG+TGG | - | chr4.4:54104007-54104026 | None:intergenic | 35.0% | |
| ATGATTGACATGGATTTGCA+GGG | + | chr4.4:54104022-54104041 | MS.gene87222:intron | 35.0% | |
| ATTCCAAATCTTACACTTCG+AGG | + | chr4.4:54103441-54103460 | MS.gene87222:CDS | 35.0% | |
| ATTGCTCATCAATTTCAACC+AGG | + | chr4.4:54104605-54104624 | MS.gene87222:CDS | 35.0% | |
| CAAGCAAGTAGTTAAGAGAA+GGG | + | chr4.4:54104783-54104802 | MS.gene87222:CDS | 35.0% | |
| GCATATTCATTGTCTAGATC+TGG | - | chr4.4:54104664-54104683 | None:intergenic | 35.0% | |
| GCCAGTAAAATTATATAGCC+TGG | - | chr4.4:54104626-54104645 | None:intergenic | 35.0% | |
| GGACGAATTAATGTCAAGAT+TGG | + | chr4.4:54104925-54104944 | MS.gene87222:CDS | 35.0% | |
| GGTTGAAATTGATGAGCAAT+GGG | - | chr4.4:54104605-54104624 | None:intergenic | 35.0% | |
| GTGAATGTAGAACTAACATC+TGG | + | chr4.4:54103350-54103369 | MS.gene87222:intron | 35.0% | |
| TGCAAGAATAGATCTTGTTG+TGG | - | chr4.4:54104845-54104864 | None:intergenic | 35.0% | |
| TGGTTGAAATTGATGAGCAA+TGG | - | chr4.4:54104606-54104625 | None:intergenic | 35.0% | |
| !! | TAGCATCGACATATTGATGT+GGG | - | chr4.4:54103195-54103214 | None:intergenic | 35.0% |
| !!! | AATTCGTCCCATTTTTTCCA+TGG | - | chr4.4:54104914-54104933 | None:intergenic | 35.0% |
| !!! | GACCGTGTCAATGTTTTTTT+AGG | - | chr4.4:54104319-54104338 | None:intergenic | 35.0% |
| AATGTCAAGATTGGGACTGA+AGG | + | chr4.4:54104934-54104953 | MS.gene87222:CDS | 40.0% | |
| ACACTTATTGAAATGGACCC+TGG | + | chr4.4:54104730-54104749 | MS.gene87222:CDS | 40.0% | |
| AGGGTCCATTTCAATAAGTG+TGG | - | chr4.4:54104731-54104750 | None:intergenic | 40.0% | |
| ATCATTACAGGTGTCCAGAT+GGG | + | chr4.4:54104241-54104260 | MS.gene87222:intron | 40.0% | |
| ATTTCAGGGATGTGATGCAT+CGG | + | chr4.4:54103374-54103393 | MS.gene87222:intron | 40.0% | |
| CAAATGTATTGCGACTTCCA+GGG | - | chr4.4:54104750-54104769 | None:intergenic | 40.0% | |
| CATGATTGACATGGATTTGC+AGG | + | chr4.4:54104021-54104040 | MS.gene87222:intron | 40.0% | |
| CTAACACTCTTTGGCAATGT+TGG | + | chr4.4:54104151-54104170 | MS.gene87222:CDS | 40.0% | |
| CTGCAAATCCATGTCAATCA+TGG | - | chr4.4:54104023-54104042 | None:intergenic | 40.0% | |
| CTGGACACCTGTAATGATAA+TGG | - | chr4.4:54104239-54104258 | None:intergenic | 40.0% | |
| GAACTAACATCTGGCATTTC+AGG | + | chr4.4:54103359-54103378 | MS.gene87222:intron | 40.0% | |
| GGAAGGTCCATGGAAAAAAT+GGG | + | chr4.4:54104904-54104923 | MS.gene87222:CDS | 40.0% | |
| TAAGCAATGCAGCATCTGAT+TGG | - | chr4.4:54104816-54104835 | None:intergenic | 40.0% | |
| TACTGAATTTGGAAGGTCCA+TGG | + | chr4.4:54104894-54104913 | MS.gene87222:CDS | 40.0% | |
| TAGCCTCGAAGTGTAAGATT+TGG | - | chr4.4:54103447-54103466 | None:intergenic | 40.0% | |
| TATCATTACAGGTGTCCAGA+TGG | + | chr4.4:54104240-54104259 | MS.gene87222:intron | 40.0% | |
| TCAAATGTATTGCGACTTCC+AGG | - | chr4.4:54104751-54104770 | None:intergenic | 40.0% | |
| TGGAAGGTCCATGGAAAAAA+TGG | + | chr4.4:54104903-54104922 | MS.gene87222:CDS | 40.0% | |
| TTGTTCTGCCATGATTGACA+TGG | + | chr4.4:54104012-54104031 | MS.gene87222:intron | 40.0% | |
| ! | AGCATCCTTTTCAGCTTGAT+TGG | - | chr4.4:54103421-54103440 | None:intergenic | 40.0% |
| ! | CAGCTTTTAGCATAGAAACC+TGG | - | chr4.4:54102997-54103016 | None:intergenic | 40.0% |
| !! | AATGACCTAGTCTTGCTTTC+TGG | + | chr4.4:54104184-54104203 | MS.gene87222:CDS | 40.0% |
| !! | CAACATTGCCAAAGAGTGTT+AGG | - | chr4.4:54104153-54104172 | None:intergenic | 40.0% |
| !! | CTAGCATCGACATATTGATG+TGG | - | chr4.4:54103196-54103215 | None:intergenic | 40.0% |
| ACTGTCCTCCTAACACTCTT+TGG | + | chr4.4:54104142-54104161 | MS.gene87222:CDS | 45.0% | |
| AGAAACACGTATGTTAGCGC+AGG | - | chr4.4:54104295-54104314 | None:intergenic | 45.0% | |
| AGGACAGTGAGGTTGTGAAA+TGG | - | chr4.4:54104130-54104149 | None:intergenic | 45.0% | |
| ATTGACATGGATTTGCAGGG+TGG | + | chr4.4:54104025-54104044 | MS.gene87222:intron | 45.0% | |
| CCTGTTGGAACCTTCCAATA+AGG | - | chr4.4:54104052-54104071 | None:intergenic | 45.0% | |
| CCTTATTGGAAGGTTCCAAC+AGG | + | chr4.4:54104049-54104068 | MS.gene87222:CDS | 45.0% | |
| CTTGTTGAGAAAGAATGCCC+TGG | + | chr4.4:54103492-54103511 | MS.gene87222:CDS | 45.0% | |
| GAAGGTTCCAACAGGTAGAA+GGG | + | chr4.4:54104057-54104076 | MS.gene87222:CDS | 45.0% | |
| GCATACCAGAAAGCAAGACT+AGG | - | chr4.4:54104192-54104211 | None:intergenic | 45.0% | |
| TGTTAGCGCAGGAGAAAAAG+CGG | - | chr4.4:54104284-54104303 | None:intergenic | 45.0% | |
| TTGACATGGATTTGCAGGGT+GGG | + | chr4.4:54104026-54104045 | MS.gene87222:intron | 45.0% | |
| TTGAGTGGAATCACGTGTGT+TGG | + | chr4.4:54103239-54103258 | MS.gene87222:intron | 45.0% | |
| ! | CAGGGCATTCTTTCTCAACA+AGG | - | chr4.4:54103494-54103513 | None:intergenic | 45.0% |
| !! | CATTGCCAAAGAGTGTTAGG+AGG | - | chr4.4:54104150-54104169 | None:intergenic | 45.0% |
| !! | TTAAAATAAAATAAAAAAGT+AGG | + | chr4.4:54104430-54104449 | MS.gene87222:intron | 5.0% |
| ACTCATGCTCAATTGCAGCC+AGG | + | chr4.4:54102976-54102995 | MS.gene87222:CDS | 50.0% | |
| ACTCCATCCCTTCTACCTGT+TGG | - | chr4.4:54104067-54104086 | None:intergenic | 50.0% | |
| AGCTGCTAGTGATGGTACAC+TGG | - | chr4.4:54103067-54103086 | None:intergenic | 50.0% | |
| ATGAGTGCAGCTGCTAGTGA+TGG | - | chr4.4:54103075-54103094 | None:intergenic | 50.0% | |
| CAGCACAAGAGACAACACCA+GGG | - | chr4.4:54103512-54103531 | None:intergenic | 50.0% | |
| GACGTGTCTAGTGTCTGACA+TGG | - | chr4.4:54103274-54103293 | None:intergenic | 50.0% | |
| GCTGCTAGTGATGGTACACT+GGG | - | chr4.4:54103066-54103085 | None:intergenic | 50.0% | |
| GGAAGGTTCCAACAGGTAGA+AGG | + | chr4.4:54104056-54104075 | MS.gene87222:CDS | 50.0% | |
| GGGAAGCCAGAGTTACTTCA+AGG | + | chr4.4:54102918-54102937 | MS.gene87222:CDS | 50.0% | |
| GGTTGTGAAATGGAGCAGGA+AGG | - | chr4.4:54104120-54104139 | None:intergenic | 50.0% | |
| GTGAGGTTGTGAAATGGAGC+AGG | - | chr4.4:54104124-54104143 | None:intergenic | 50.0% | |
| GTTCCAACAGGTAGAAGGGA+TGG | + | chr4.4:54104061-54104080 | MS.gene87222:CDS | 50.0% | |
| TCAGCACAAGAGACAACACC+AGG | - | chr4.4:54103513-54103532 | None:intergenic | 50.0% | |
| ! | AGAGTGTTAGGAGGACAGTG+AGG | - | chr4.4:54104141-54104160 | None:intergenic | 50.0% |
| ! | ATTTGCAGGGTGGGCCTTAT+TGG | + | chr4.4:54104035-54104054 | MS.gene87222:intron | 50.0% |
| ! | CTTTCAGGTGCGCACACAAT+TGG | + | chr4.4:54104574-54104593 | MS.gene87222:intron | 50.0% |
| ACGGCGCGCGAGAAATTCAT+AGG | + | chr4.4:54103700-54103719 | MS.gene87222:intron | 55.0% | |
| CAGGAGAAAAAGCGGTCAGG+TGG | - | chr4.4:54104276-54104295 | None:intergenic | 55.0% | |
| GCGCAGGAGAAAAAGCGGTC+AGG | - | chr4.4:54104279-54104298 | None:intergenic | 60.0% | |
| !! | GCAGGGTGGGCCTTATTGGA+AGG | + | chr4.4:54104039-54104058 | MS.gene87222:intron | 60.0% |
| GGTGGACACTCGTGCCCATC+TGG | - | chr4.4:54104258-54104277 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 54102910 | 54104990 | 54102910 | ID=MS.gene87222 |
| chr4.4 | mRNA | 54102910 | 54104990 | 54102910 | ID=MS.gene87222.t1;Parent=MS.gene87222 |
| chr4.4 | exon | 54102910 | 54103125 | 54102910 | ID=MS.gene87222.t1.exon1;Parent=MS.gene87222.t1 |
| chr4.4 | CDS | 54102910 | 54103125 | 54102910 | ID=cds.MS.gene87222.t1;Parent=MS.gene87222.t1 |
| chr4.4 | exon | 54103381 | 54103572 | 54103381 | ID=MS.gene87222.t1.exon2;Parent=MS.gene87222.t1 |
| chr4.4 | CDS | 54103381 | 54103572 | 54103381 | ID=cds.MS.gene87222.t1;Parent=MS.gene87222.t1 |
| chr4.4 | exon | 54104043 | 54104205 | 54104043 | ID=MS.gene87222.t1.exon3;Parent=MS.gene87222.t1 |
| chr4.4 | CDS | 54104043 | 54104205 | 54104043 | ID=cds.MS.gene87222.t1;Parent=MS.gene87222.t1 |
| chr4.4 | exon | 54104581 | 54104990 | 54104581 | ID=MS.gene87222.t1.exon4;Parent=MS.gene87222.t1 |
| chr4.4 | CDS | 54104581 | 54104990 | 54104581 | ID=cds.MS.gene87222.t1;Parent=MS.gene87222.t1 |
| Gene Sequence |
| Protein sequence |
