AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92217


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92217.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 9.44e-124 346
MS.gene92217.t1 MTR_7g007120 95.294 170 8 0 12 181 12 181 1.95e-123 345
MS.gene92217.t1 MTR_7g007220 94.737 171 9 0 11 181 11 181 2.03e-123 345
MS.gene92217.t1 MTR_7g007200 94.152 171 10 0 11 181 11 181 2.06e-123 345
MS.gene92217.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 5.40e-123 344
MS.gene92217.t1 MTR_7g007230 93.529 170 11 0 12 181 12 181 4.85e-121 339
MS.gene92217.t1 MTR_6g018310 85.475 179 25 1 4 181 2 180 2.24e-115 325
MS.gene92217.t1 MTR_6g018300 83.240 179 26 2 4 181 2 177 1.98e-110 312
MS.gene92217.t1 MTR_7g007210 94.805 154 8 0 11 164 11 164 9.35e-110 311
MS.gene92217.t1 MTR_4g127870 49.359 156 75 2 28 180 15 169 1.01e-56 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92217.t1 AT1G67090 70.330 182 49 2 1 181 1 178 4.37e-93 269
MS.gene92217.t1 AT5G38410 70.330 182 49 2 1 181 1 178 4.83e-93 268
MS.gene92217.t1 AT5G38430 70.330 182 49 2 1 181 1 178 1.25e-92 268
MS.gene92217.t1 AT5G38430 70.330 182 49 2 1 181 1 178 1.25e-92 268
MS.gene92217.t1 AT5G38420 69.780 182 50 2 1 181 1 178 9.31e-92 265
MS.gene92217.t1 AT5G38410 68.449 187 49 3 1 181 1 183 6.80e-91 263
MS.gene92217.t1 AT5G38410 67.582 182 47 3 1 181 1 171 2.96e-86 251
MS.gene92217.t1 AT1G67090 64.348 115 36 2 1 114 1 111 4.63e-45 145

Find 53 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCCATCCCTTCCTTATA+AGG 0.282748 7.1:+87135973 None:intergenic
AAGAAGTTGAATACCTTATA+AGG 0.317300 7.1:-87135986 MS.gene92217:CDS
TCTTTGTTGACCTTTGTAAC+TGG 0.323260 7.1:+87136383 None:intergenic
GATACTATGACGGACGTTAC+TGG 0.345500 7.1:-87135731 MS.gene92217:CDS
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.1:-87135964 MS.gene92217:CDS
TTCTTTCGCCAATTGTTCTC+TGG 0.378466 7.1:+87136004 None:intergenic
GACCTTTGTAACTGGGAAAC+TGG 0.392333 7.1:+87136391 None:intergenic
GCCAATTGTTCTCTGGTCAA+TGG 0.405140 7.1:+87136011 None:intergenic
ACCATTGACCAGAGAACAAT+TGG 0.409622 7.1:-87136012 MS.gene92217:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.1:-87135705 MS.gene92217:CDS
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.1:-87136459 MS.gene92217:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435101 7.1:+87136053 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.1:+87135627 None:intergenic
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.1:-87135624 MS.gene92217:CDS
GGCGCAGTGGCTCCATTCGT+TGG 0.489747 7.1:-87136428 MS.gene92217:CDS
CTTTGTTGACCTTTGTAACT+GGG 0.508579 7.1:+87136384 None:intergenic
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.1:+87136056 None:intergenic
CGTGAGAACCACAGTTCACC+AGG 0.516570 7.1:-87135753 MS.gene92217:CDS
AATACCTTATAAGGAAGGGA+TGG 0.520774 7.1:-87135977 MS.gene92217:CDS
CGTGCCTCTTCGGTACAATC+CGG 0.524408 7.1:-87136449 MS.gene92217:CDS
AGTAAAACTTAGTAAACTGC+TGG 0.535526 7.1:+87135552 None:intergenic
AGTTGAATACCTTATAAGGA+AGG 0.536755 7.1:-87135982 MS.gene92217:CDS
TCTTCCACCATTGCTTGCAA+TGG 0.537419 7.1:+87136352 None:intergenic
CGATGCTTCTCAAGTGCTGA+AGG 0.539995 7.1:-87135676 MS.gene92217:CDS
AACGAATGGAGCCACTGCGC+CGG 0.545146 7.1:+87136430 None:intergenic
TGAAACTGATGCATTGAACT+TGG 0.551147 7.1:+87135586 None:intergenic
CTTCAGCACTTGAGAAGCAT+CGG 0.558039 7.1:+87135677 None:intergenic
TAACGTCCGTCATAGTATCC+TGG 0.560465 7.1:+87135735 None:intergenic
ACTTCCATTGCAAGCAATGG+TGG 0.573273 7.1:-87136356 MS.gene92217:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.1:+87135613 None:intergenic
AACTGTAGTCATAGCTGAAG+AGG 0.581913 7.1:+87136475 None:intergenic
ATTACTTCCATTGCAAGCAA+TGG 0.582522 7.1:-87136359 MS.gene92217:CDS
TGTAACTGGGAAACTGGCCA+TGG 0.582656 7.1:+87136397 None:intergenic
CTGGTGAACTGTGGTTCTCA+CGG 0.583102 7.1:+87135754 None:intergenic
GGCCAGTTTCCCAGTTACAA+AGG 0.584504 7.1:-87136393 MS.gene92217:CDS
ATACCTTATAAGGAAGGGAT+GGG 0.588445 7.1:-87135976 MS.gene92217:CDS
GTTGAATACCTTATAAGGAA+GGG 0.588887 7.1:-87135981 MS.gene92217:CDS
GCATCGGTTGCTCCAAACAA+AGG 0.600691 7.1:+87135693 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.600961 7.1:+87136014 None:intergenic
ATTCGTTGGACTCAAATCCA+TGG 0.603675 7.1:-87136414 MS.gene92217:CDS
TTCGGTACAATCCGGCGCAG+TGG 0.604437 7.1:-87136441 MS.gene92217:CDS
AGCTGAAGAGGAAATCATAG+AGG 0.609922 7.1:+87136487 None:intergenic
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.1:-87135949 MS.gene92217:intron
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.1:+87136018 None:intergenic
TGGAAGAGTAAGCTGCATGC+AGG 0.622787 7.1:-87136336 MS.gene92217:intron
ATGGATTTGAGTCCAACGAA+TGG 0.627592 7.1:+87136416 None:intergenic
CATAGTATCCTGGTGAACTG+TGG 0.641120 7.1:+87135745 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.641462 7.1:+87136450 None:intergenic
TGATACGGACAAAGGCATCA+GGG 0.656050 7.1:+87135628 None:intergenic
AGTTCACCAGGATACTATGA+CGG 0.676016 7.1:-87135741 MS.gene92217:CDS
GAATCCGATGATACGGACAA+AGG 0.687909 7.1:+87135620 None:intergenic
TGCGCCGGATTGTACCGAAG+AGG 0.688359 7.1:+87136445 None:intergenic
ACGGACGTTACTGGACAATG+TGG 0.708236 7.1:-87135722 MS.gene92217:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTATTAGCATAA+TGG - chr7.1:87135764-87135783 MS.gene92217:CDS 10.0%
!!! TATATATAGTTTTGAACTTA+TGG - chr7.1:87136167-87136186 MS.gene92217:intron 15.0%
!! AGTAAATTAAAATTAGACCT+CGG + chr7.1:87136125-87136144 None:intergenic 20.0%
!! TATAAGATGAAAACAGTTTA+CGG + chr7.1:87135808-87135827 None:intergenic 20.0%
!!! CTCAAAAGGATTTTAAATTA+AGG + chr7.1:87136230-87136249 None:intergenic 20.0%
! AAGAAGTTGAATACCTTATA+AGG - chr7.1:87136068-87136087 MS.gene92217:intron 25.0%
!! TGACTCTTTTGTTCAATTTT+AGG - chr7.1:87135970-87135989 MS.gene92217:CDS 25.0%
!!! AAAATCCTTTTGAGTTATGA+TGG - chr7.1:87136236-87136255 MS.gene92217:intron 25.0%
!!! GTTTTGAACTTATGGTTAAT+CGG - chr7.1:87136175-87136194 MS.gene92217:intron 25.0%
AGTTGAATACCTTATAAGGA+AGG - chr7.1:87136072-87136091 MS.gene92217:intron 30.0%
GTTAACCATCATAACTCAAA+AGG + chr7.1:87136244-87136263 None:intergenic 30.0%
GTTGAATACCTTATAAGGAA+GGG - chr7.1:87136073-87136092 MS.gene92217:intron 30.0%
!! CTTTTGTTCAATTTTAGGTG+TGG - chr7.1:87135975-87135994 MS.gene92217:CDS 30.0%
AATACCTTATAAGGAAGGGA+TGG - chr7.1:87136077-87136096 MS.gene92217:intron 35.0%
AATCCTTTCTGCAAATAAGC+AGG + chr7.1:87136274-87136293 None:intergenic 35.0%
ATACCTTATAAGGAAGGGAT+GGG - chr7.1:87136078-87136097 MS.gene92217:intron 35.0%
ATTACTTCCATTGCAAGCAA+TGG - chr7.1:87135695-87135714 MS.gene92217:CDS 35.0%
CTTTGTTGACCTTTGTAACT+GGG + chr7.1:87135673-87135692 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.1:87136004-87136023 None:intergenic 35.0%
TCTTTGTTGACCTTTGTAAC+TGG + chr7.1:87135674-87135693 None:intergenic 35.0%
TGAAACTGATGCATTGAACT+TGG + chr7.1:87136471-87136490 None:intergenic 35.0%
AACTGTAGTCATAGCTGAAG+AGG + chr7.1:87135582-87135601 None:intergenic 40.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.1:87136001-87136020 None:intergenic 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.1:87136043-87136062 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.1:87136042-87136061 MS.gene92217:CDS 40.0%
AGCTGAAGAGGAAATCATAG+AGG + chr7.1:87135570-87135589 None:intergenic 40.0%
AGTTCACCAGGATACTATGA+CGG - chr7.1:87136313-87136332 MS.gene92217:intron 40.0%
ATGGATTTGAGTCCAACGAA+TGG + chr7.1:87135641-87135660 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.1:87136349-87136368 MS.gene92217:CDS 40.0%
ATTCGTTGGACTCAAATCCA+TGG - chr7.1:87135640-87135659 MS.gene92217:CDS 40.0%
CTACCTGCTTATTTGCAGAA+AGG - chr7.1:87136268-87136287 MS.gene92217:intron 40.0%
GTTTGTCATGAATCAGATCG+CGG - chr7.1:87136197-87136216 MS.gene92217:intron 40.0%
TTCTTTCGCCAATTGTTCTC+TGG + chr7.1:87136053-87136072 None:intergenic 40.0%
ACTTCCATTGCAAGCAATGG+TGG - chr7.1:87135698-87135717 MS.gene92217:CDS 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.1:87136084-87136103 None:intergenic 45.0%
CATAGTATCCTGGTGAACTG+TGG + chr7.1:87136312-87136331 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.1:87136444-87136463 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.1:87136437-87136456 None:intergenic 45.0%
GACCTTTGTAACTGGGAAAC+TGG + chr7.1:87135666-87135685 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.1:87136430-87136449 MS.gene92217:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.1:87136046-87136065 None:intergenic 45.0%
TAACGTCCGTCATAGTATCC+TGG + chr7.1:87136322-87136341 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.1:87135595-87135614 MS.gene92217:CDS 45.0%
TCTTCCACCATTGCTTGCAA+TGG + chr7.1:87135705-87135724 None:intergenic 45.0%
! CTTCAGCACTTGAGAAGCAT+CGG + chr7.1:87136380-87136399 None:intergenic 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.1:87136105-87136124 MS.gene92217:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.1:87136430-87136449 None:intergenic 45.0%
!! GATACTATGACGGACGTTAC+TGG - chr7.1:87136323-87136342 MS.gene92217:intron 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.1:87136429-87136448 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.1:87135986-87136005 MS.gene92217:CDS 45.0%
!! TAATAATAAAATAAAATAGT+AGG + chr7.1:87135758-87135777 None:intergenic 5.0%
ACGGACGTTACTGGACAATG+TGG - chr7.1:87136332-87136351 MS.gene92217:intron 50.0%
CATGAATCAGATCGCGGTCA+CGG - chr7.1:87136203-87136222 MS.gene92217:intron 50.0%
CTGGTGAACTGTGGTTCTCA+CGG + chr7.1:87136303-87136322 None:intergenic 50.0%
GGCCAGTTTCCCAGTTACAA+AGG - chr7.1:87135661-87135680 MS.gene92217:CDS 50.0%
TGGAAGAGTAAGCTGCATGC+AGG - chr7.1:87135718-87135737 MS.gene92217:CDS 50.0%
TGTAACTGGGAAACTGGCCA+TGG + chr7.1:87135660-87135679 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.1:87136039-87136058 None:intergenic 50.0%
!! CGATGCTTCTCAAGTGCTGA+AGG - chr7.1:87136378-87136397 MS.gene92217:CDS 50.0%
!! GCATCGGTTGCTCCAAACAA+AGG + chr7.1:87136364-87136383 None:intergenic 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.1:87135607-87135626 None:intergenic 55.0%
CGTGAGAACCACAGTTCACC+AGG - chr7.1:87136301-87136320 MS.gene92217:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.1:87135605-87135624 MS.gene92217:CDS 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.1:87136090-87136109 MS.gene92217:intron 55.0%
AACGAATGGAGCCACTGCGC+CGG + chr7.1:87135627-87135646 None:intergenic 60.0%
TGCGCCGGATTGTACCGAAG+AGG + chr7.1:87135612-87135631 None:intergenic 60.0%
TTCGGTACAATCCGGCGCAG+TGG - chr7.1:87135613-87135632 MS.gene92217:CDS 60.0%
GGCGCAGTGGCTCCATTCGT+TGG - chr7.1:87135626-87135645 MS.gene92217:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 87135560 87136516 87135560 ID=MS.gene92217
chr7.1 mRNA 87135560 87136516 87135560 ID=MS.gene92217.t1;Parent=MS.gene92217
chr7.1 exon 87136337 87136516 87136337 ID=MS.gene92217.t1.exon1;Parent=MS.gene92217.t1
chr7.1 CDS 87136337 87136516 87136337 ID=cds.MS.gene92217.t1;Parent=MS.gene92217.t1
chr7.1 exon 87135950 87136084 87135950 ID=MS.gene92217.t1.exon2;Parent=MS.gene92217.t1
chr7.1 CDS 87135950 87136084 87135950 ID=cds.MS.gene92217.t1;Parent=MS.gene92217.t1
chr7.1 exon 87135560 87135790 87135560 ID=MS.gene92217.t1.exon3;Parent=MS.gene92217.t1
chr7.1 CDS 87135560 87135790 87135560 ID=cds.MS.gene92217.t1;Parent=MS.gene92217.t1
Gene Sequence

>MS.gene92217

ATGGCTTCCTCTATGATTTCCTCTTCAGCTATGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGCGCAGTGGCTCCATTCGTTGGACTCAAATCCATGGCCAGTTTCCCAGTTACAAAGGTCAACAAAGACATTACTTCCATTGCAAGCAATGGTGGAAGAGTAAGCTGCATGCAGGTGAGAAAAAACATGCCTACTATTTTATTTTATTATTAGCATAATGGAAACAATTTATGACATGTCCGTAAACTGTTTTCATCTTATATTTTCATAAGCTCTTCATGATAGTTTATGAAAACAGCTTATAGACTATATGAAAAAGTTTGACTTTAATTTATCTTTTGTCATAAAAATAACTTATACATATAAGTAATTAGAGATTCATTGAAAAATGCACACAAGTTTGTTGACTCTTTTGTTCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCGAAAGAAGTTGAATACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATTTTAATTTACTTTCAATATCTATTCAATGATCATATATATAGTTTTGAACTTATGGTTAATCGGTTTGTCATGAATCAGATCGCGGTCACGGACCTTAATTTAAAATCCTTTTGAGTTATGATGGTTAACATGTCTACCTGCTTATTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAGTTCACCAGGATACTATGACGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATGCTTCTCAAGTGCTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGCCAAGTTCAATGCATCAGTTTCATTGCACACACACCAGCAGTTTACTAA

Protein sequence

>MS.gene92217.t1

MASSMISSSAMTTVNRASSVQSGAVAPFVGLKSMASFPVTKVNKDITSIASNGGRVSCMQVWPPIGKKKFETLSYLPPLTREQLAKEVEYLIRKGWVACLEFETEKGFVYRENHSSPGYYDGRYWTMWKLPLFGATDASQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY