AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76905


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76905.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 1.99e-124 348
MS.gene76905.t1 MTR_7g007200 94.152 171 10 0 11 181 11 181 3.29e-124 347
MS.gene76905.t1 MTR_7g007220 94.737 171 9 0 11 181 11 181 3.80e-124 347
MS.gene76905.t1 MTR_7g007120 94.706 170 9 0 12 181 12 181 8.46e-124 346
MS.gene76905.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 1.08e-123 346
MS.gene76905.t1 MTR_7g007230 92.941 170 12 0 12 181 12 181 5.72e-121 339
MS.gene76905.t1 MTR_6g018310 84.916 179 26 1 4 181 2 180 1.72e-114 323
MS.gene76905.t1 MTR_6g018300 84.358 179 24 2 4 181 2 177 9.49e-112 316
MS.gene76905.t1 MTR_7g007210 94.805 154 8 0 11 164 11 164 2.51e-110 313
MS.gene76905.t1 MTR_4g127870 50.641 156 73 3 28 180 15 169 1.12e-57 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76905.t1 AT1G67090 71.429 182 47 2 1 181 1 178 1.08e-93 270
MS.gene76905.t1 AT5G38430 70.879 182 48 2 1 181 1 178 1.06e-92 268
MS.gene76905.t1 AT5G38430 70.879 182 48 2 1 181 1 178 1.06e-92 268
MS.gene76905.t1 AT5G38410 70.330 182 49 2 1 181 1 178 2.07e-92 267
MS.gene76905.t1 AT5G38420 70.330 182 49 2 1 181 1 178 6.85e-92 266
MS.gene76905.t1 AT5G38410 68.449 187 49 3 1 181 1 183 3.68e-90 261
MS.gene76905.t1 AT5G38410 67.582 182 47 3 1 181 1 171 1.28e-85 249
MS.gene76905.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.72e-45 146

Find 52 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.242109 7.2:-91241862 MS.gene76905:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.2:+91241849 None:intergenic
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.2:+91242164 None:intergenic
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.2:-91241840 MS.gene76905:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.2:-91241639 MS.gene76905:CDS
TTCTTTAGCCAATTGTTCTC+TGG 0.392107 7.2:+91241880 None:intergenic
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.2:-91241584 MS.gene76905:CDS
TCTTCCACCATTGCTTGTAA+TGG 0.401668 7.2:+91242133 None:intergenic
ACCATTGACCAGAGAACAAT+TGG 0.402581 7.2:-91241888 MS.gene76905:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.2:-91241613 MS.gene76905:CDS
GCCAATTGTTCTCTGGTCAA+TGG 0.422643 7.2:+91241887 None:intergenic
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.2:-91242240 MS.gene76905:CDS
ATTCGTTGGACTCAAGTCTA+TGG 0.435719 7.2:-91242195 MS.gene76905:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435806 7.2:+91241929 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.2:+91241535 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.2:+91241585 None:intergenic
GGCGCGGTGGCTCCATTCGT+TGG 0.461846 7.2:-91242209 MS.gene76905:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.2:-91241532 MS.gene76905:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.2:+91242165 None:intergenic
CTCAAAATTTAGTAGACTGC+AGG 0.495678 7.2:+91241460 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.2:-91241853 MS.gene76905:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.2:+91241932 None:intergenic
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.2:-91241858 MS.gene76905:CDS
CGTGCCTCTTCGGTACAATC+CGG 0.524408 7.2:-91242230 MS.gene76905:CDS
GTTGGACTCAAGTCTATGGC+TGG 0.525967 7.2:-91242191 MS.gene76905:CDS
TCCACCATTGCTTGTAATGG+AGG 0.530318 7.2:+91242136 None:intergenic
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.2:+91241653 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.2:-91241661 MS.gene76905:CDS
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.2:-91241852 MS.gene76905:CDS
AACGAATGGAGCCACCGCGC+CGG 0.560979 7.2:+91242211 None:intergenic
AGCTGAAGAGGACATGATAG+AGG 0.561007 7.2:+91242268 None:intergenic
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.2:-91241857 MS.gene76905:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.2:+91241643 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.2:-91241649 MS.gene76905:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.2:+91241521 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.2:+91241662 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.597051 7.2:+91242256 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.2:+91241601 None:intergenic
ATTACCTCCATTACAAGCAA+TGG 0.604904 7.2:-91242140 MS.gene76905:CDS
AATTGTTCTCTGGTCAATGG+TGG 0.606631 7.2:+91241890 None:intergenic
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.2:-91241825 MS.gene76905:intron
ACCTCCATTACAAGCAATGG+TGG 0.613355 7.2:-91242137 MS.gene76905:CDS
TTCGGTACAATCCGGCGCGG+TGG 0.614407 7.2:-91242222 MS.gene76905:CDS
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.2:+91241894 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.641462 7.2:+91242231 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.2:-91242117 MS.gene76905:intron
ATAGACTTGAGTCCAACGAA+TGG 0.654896 7.2:+91242197 None:intergenic
TGATACGGACAAAGGCATCA+GGG 0.656050 7.2:+91241536 None:intergenic
CGCGCCGGATTGTACCGAAG+AGG 0.661856 7.2:+91242226 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.2:+91241528 None:intergenic
CTCTTCGGTACAATCCGGCG+CGG 0.696951 7.2:-91242225 MS.gene76905:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.2:-91241630 MS.gene76905:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACCATTTTGTGAAATTTT+AGG - chr7.2:91241783-91241802 MS.gene76905:intron 20.0%
! ACACCTAAAATTTCACAAAA+TGG + chr7.2:91241789-91241808 None:intergenic 25.0%
! AGTAAATGAATATTAGACCT+CGG + chr7.2:91241938-91241957 None:intergenic 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.2:91241881-91241900 MS.gene76905:CDS 25.0%
!! TATATTTTCATAAGCTCTTC+AGG - chr7.2:91241690-91241709 MS.gene76905:intron 25.0%
!!! ATTTTGTGAAATTTTAGGTG+TGG - chr7.2:91241788-91241807 MS.gene76905:intron 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.2:91242034-91242053 MS.gene76905:intron 25.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.2:91241885-91241904 MS.gene76905:CDS 30.0%
ATAAGCACTTATAGATTCGT+TGG - chr7.2:91241741-91241760 MS.gene76905:intron 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.2:91241581-91241600 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.2:91241886-91241905 MS.gene76905:CDS 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.2:91241582-91241601 None:intergenic 30.0%
ATTACCTCCATTACAAGCAA+TGG - chr7.2:91241603-91241622 MS.gene76905:CDS 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.2:91241890-91241909 MS.gene76905:CDS 35.0%
CTTCAACACTTGAGAAGAAT+CGG + chr7.2:91242161-91242180 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.2:91241817-91241836 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.2:91241891-91241910 MS.gene76905:CDS 35.0%
TTCTTTAGCCAATTGTTCTC+TGG + chr7.2:91241866-91241885 None:intergenic 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.2:91241814-91241833 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.2:91242094-91242113 MS.gene76905:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.2:91241856-91241875 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.2:91241855-91241874 MS.gene76905:CDS 40.0%
ATAGACTTGAGTCCAACGAA+TGG + chr7.2:91241549-91241568 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.2:91242130-91242149 MS.gene76905:CDS 40.0%
ATTCGTTGGACTCAAGTCTA+TGG - chr7.2:91241548-91241567 MS.gene76905:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.2:91242003-91242022 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.2:91242049-91242068 MS.gene76905:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.2:91242103-91242122 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.2:91241613-91241632 None:intergenic 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.2:91242159-91242178 MS.gene76905:CDS 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.2:91242104-91242123 MS.gene76905:intron 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.2:91241490-91241509 None:intergenic 45.0%
ACCTCCATTACAAGCAATGG+TGG - chr7.2:91241606-91241625 MS.gene76905:CDS 45.0%
AGCTGAAGAGGACATGATAG+AGG + chr7.2:91241478-91241497 None:intergenic 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.2:91242113-91242132 MS.gene76905:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.2:91241897-91241916 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.2:91242093-91242112 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.2:91242225-91242244 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.2:91242218-91242237 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.2:91242145-91242164 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.2:91242211-91242230 MS.gene76905:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.2:91241859-91241878 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.2:91241503-91241522 MS.gene76905:CDS 45.0%
TCCACCATTGCTTGTAATGG+AGG + chr7.2:91241610-91241629 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.2:91241626-91241645 MS.gene76905:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.2:91241918-91241937 MS.gene76905:CDS 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.2:91242211-91242230 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.2:91242210-91242229 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.2:91241799-91241818 MS.gene76905:intron 45.0%
GTTGGACTCAAGTCTATGGC+TGG - chr7.2:91241552-91241571 MS.gene76905:CDS 50.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.2:91242084-91242103 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.2:91241852-91241871 None:intergenic 50.0%
!! TCGTTGTTTTGTCGCGACCA+AGG - chr7.2:91241983-91242002 MS.gene76905:intron 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.2:91241515-91241534 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.2:91242082-91242101 MS.gene76905:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.2:91241513-91241532 MS.gene76905:CDS 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.2:91241903-91241922 MS.gene76905:CDS 55.0%
CTCTTCGGTACAATCCGGCG+CGG - chr7.2:91241518-91241537 MS.gene76905:CDS 60.0%
AACGAATGGAGCCACCGCGC+CGG + chr7.2:91241535-91241554 None:intergenic 65.0%
CGCGCCGGATTGTACCGAAG+AGG + chr7.2:91241520-91241539 None:intergenic 65.0%
TTCGGTACAATCCGGCGCGG+TGG - chr7.2:91241521-91241540 MS.gene76905:CDS 65.0%
GGCGCGGTGGCTCCATTCGT+TGG - chr7.2:91241534-91241553 MS.gene76905:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 91241468 91242297 91241468 ID=MS.gene76905
chr7.2 mRNA 91241468 91242297 91241468 ID=MS.gene76905.t1;Parent=MS.gene76905
chr7.2 exon 91242118 91242297 91242118 ID=MS.gene76905.t1.exon1;Parent=MS.gene76905.t1
chr7.2 CDS 91242118 91242297 91242118 ID=cds.MS.gene76905.t1;Parent=MS.gene76905.t1
chr7.2 exon 91241826 91241960 91241826 ID=MS.gene76905.t1.exon2;Parent=MS.gene76905.t1
chr7.2 CDS 91241826 91241960 91241826 ID=cds.MS.gene76905.t1;Parent=MS.gene76905.t1
chr7.2 exon 91241468 91241698 91241468 ID=MS.gene76905.t1.exon3;Parent=MS.gene76905.t1
chr7.2 CDS 91241468 91241698 91241468 ID=cds.MS.gene76905.t1;Parent=MS.gene76905.t1
Gene Sequence

>MS.gene76905

ATGGCTTCCTCTATCATGTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGCGCGGTGGCTCCATTCGTTGGACTCAAGTCTATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACCTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAAAATTTATGACATGTCTGTAAACTGTTTTCATCTTATATTTTCATAAGCTCTTCAGGATAGTTTATGAAAACAGCTTATACGTACATAAGCACTTATAGATTCGTTGGCAAAAGTACACAAGTTTGTTAACCATTTTGTGAAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCTAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTCATTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGACCAAGGTCACAGTCACAAATTTGTACTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene76905.t1

MASSIMSSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY