AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92216


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92216.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 1.23e-123 346
MS.gene92216.t1 MTR_7g007200 93.567 171 11 0 11 181 11 181 2.25e-123 345
MS.gene92216.t1 MTR_7g007220 94.152 171 10 0 11 181 11 181 2.27e-123 345
MS.gene92216.t1 MTR_7g007120 94.118 170 10 0 12 181 12 181 4.95e-123 344
MS.gene92216.t1 MTR_7g007120 93.567 171 11 0 11 181 11 181 6.37e-123 344
MS.gene92216.t1 MTR_7g007230 92.353 170 13 0 12 181 12 181 3.57e-120 337
MS.gene92216.t1 MTR_6g018310 83.799 179 28 1 4 181 2 180 7.16e-114 321
MS.gene92216.t1 MTR_6g018300 83.240 179 26 2 4 181 2 177 4.81e-111 314
MS.gene92216.t1 MTR_7g007210 94.156 154 9 0 11 164 11 164 1.65e-109 310
MS.gene92216.t1 MTR_4g127870 48.810 168 81 4 17 180 3 169 3.92e-58 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92216.t1 AT1G67090 70.330 182 49 2 1 181 1 178 6.21e-93 268
MS.gene92216.t1 AT5G38430 69.780 182 50 2 1 181 1 178 6.01e-92 266
MS.gene92216.t1 AT5G38430 69.780 182 50 2 1 181 1 178 6.01e-92 266
MS.gene92216.t1 AT5G38410 69.231 182 51 2 1 181 1 178 1.24e-91 265
MS.gene92216.t1 AT5G38420 69.231 182 51 2 1 181 1 178 3.70e-91 264
MS.gene92216.t1 AT5G38410 67.380 187 51 3 1 181 1 183 2.39e-89 259
MS.gene92216.t1 AT5G38410 66.484 182 49 3 1 181 1 171 1.02e-84 247
MS.gene92216.t1 AT1G67090 64.348 115 36 2 1 114 1 111 3.97e-45 145

Find 49 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.244592 7.1:-87145052 MS.gene92216:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.1:+87145039 None:intergenic
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.1:+87145354 None:intergenic
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.1:-87145030 MS.gene92216:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.1:-87144829 MS.gene92216:CDS
ACCATTGACCAGAGAACAAT+TGG 0.397843 7.1:-87145078 MS.gene92216:CDS
TGATGCTTCTCAAGTGTTGA+AGG 0.410018 7.1:-87144774 MS.gene92216:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.1:-87144803 MS.gene92216:CDS
GCCAATTGTTCTCTGGTCAA+TGG 0.416317 7.1:+87145077 None:intergenic
TCTTCCACCATTGCTTGTAA+TGG 0.422658 7.1:+87145323 None:intergenic
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.1:-87145430 MS.gene92216:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435806 7.1:+87145119 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.1:+87144725 None:intergenic
GGCGTGGCGGCTCCATTCGT+TGG 0.466438 7.1:-87145399 MS.gene92216:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.1:-87144722 MS.gene92216:CDS
CGTGCCTCTTCGGTACAATC+TGG 0.487772 7.1:-87145420 MS.gene92216:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.1:+87145355 None:intergenic
CTCAAAATTTAGTAGACTGC+AGG 0.495678 7.1:+87144650 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.1:-87145043 MS.gene92216:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.1:+87145122 None:intergenic
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.1:-87145048 MS.gene92216:CDS
ATTACTTCCATTACAAGCAA+TGG 0.539617 7.1:-87145330 MS.gene92216:CDS
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.1:+87144843 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.1:-87144851 MS.gene92216:CDS
GTTGGACTAAAGTCCATGGC+TGG 0.555609 7.1:-87145381 MS.gene92216:CDS
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.1:-87145042 MS.gene92216:CDS
AGCTGAAGAGGACATGATAG+AGG 0.561007 7.1:+87145458 None:intergenic
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.1:-87145047 MS.gene92216:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.1:+87144833 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.1:-87144839 MS.gene92216:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.1:+87144711 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.1:+87144852 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.597051 7.1:+87145446 None:intergenic
GCATCAGTTGCTCCAAACAA+AGG 0.598394 7.1:+87144791 None:intergenic
CACGCCAGATTGTACCGAAG+AGG 0.605699 7.1:+87145416 None:intergenic
ATTCGTTGGACTAAAGTCCA+TGG 0.609669 7.1:-87145385 MS.gene92216:CDS
ACTTCCATTACAAGCAATGG+TGG 0.610706 7.1:-87145327 MS.gene92216:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.1:-87145015 MS.gene92216:intron
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.1:+87145368 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.1:+87145084 None:intergenic
CTCTTCGGTACAATCTGGCG+TGG 0.620770 7.1:-87145415 MS.gene92216:CDS
ATGGACTTTAGTCCAACGAA+TGG 0.623442 7.1:+87145387 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.637841 7.1:+87145080 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.1:-87145307 MS.gene92216:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.1:+87144726 None:intergenic
CAGATTGTACCGAAGAGGCA+CGG 0.664722 7.1:+87145421 None:intergenic
TTCGGTACAATCTGGCGTGG+CGG 0.667454 7.1:-87145412 MS.gene92216:CDS
GAATCCGATGATACGGACAA+AGG 0.687909 7.1:+87144718 None:intergenic
ATGGACGTTACTGGACAATG+TGG 0.742197 7.1:-87144820 MS.gene92216:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTAAAGTAATATTAGACCT+CGG + chr7.1:87145128-87145147 None:intergenic 25.0%
! ATAAGCACTTATAGATTCAT+TGG - chr7.1:87144931-87144950 MS.gene92216:intron 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.1:87145071-87145090 MS.gene92216:CDS 25.0%
!! TATATTTTCATAAGCTCTTC+AGG - chr7.1:87144880-87144899 MS.gene92216:intron 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.1:87145224-87145243 MS.gene92216:intron 25.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.1:87145075-87145094 MS.gene92216:CDS 30.0%
ATTACTTCCATTACAAGCAA+TGG - chr7.1:87144793-87144812 MS.gene92216:CDS 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.1:87144771-87144790 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.1:87145076-87145095 MS.gene92216:CDS 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.1:87144772-87144791 None:intergenic 30.0%
!!! ACAAGTTTGTTAACCCTTTT+GGG - chr7.1:87144963-87144982 MS.gene92216:intron 30.0%
ACACCTAAAATTGCCCAAAA+GGG + chr7.1:87144979-87144998 None:intergenic 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.1:87145080-87145099 MS.gene92216:CDS 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.1:87145007-87145026 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.1:87145081-87145100 MS.gene92216:CDS 35.0%
! TAACCCTTTTGGGCAATTTT+AGG - chr7.1:87144973-87144992 MS.gene92216:intron 35.0%
! TTCTTTTGCCAATTGTTCTC+TGG + chr7.1:87145056-87145075 None:intergenic 35.0%
!!! CACAAGTTTGTTAACCCTTT+TGG - chr7.1:87144962-87144981 MS.gene92216:intron 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.1:87145004-87145023 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.1:87145284-87145303 MS.gene92216:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.1:87145046-87145065 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.1:87145045-87145064 MS.gene92216:CDS 40.0%
ACTTCCATTACAAGCAATGG+TGG - chr7.1:87144796-87144815 MS.gene92216:CDS 40.0%
ATGGACTTTAGTCCAACGAA+TGG + chr7.1:87144739-87144758 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.1:87145320-87145339 MS.gene92216:CDS 40.0%
ATTCGTTGGACTAAAGTCCA+TGG - chr7.1:87144738-87144757 MS.gene92216:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.1:87145193-87145212 None:intergenic 40.0%
CACACCTAAAATTGCCCAAA+AGG + chr7.1:87144980-87144999 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.1:87145239-87145258 MS.gene92216:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.1:87145293-87145312 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.1:87144803-87144822 None:intergenic 40.0%
!! CTTTTGGGCAATTTTAGGTG+TGG - chr7.1:87144978-87144997 MS.gene92216:intron 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.1:87145294-87145313 MS.gene92216:intron 40.0%
!! TGATGCTTCTCAAGTGTTGA+AGG - chr7.1:87145349-87145368 MS.gene92216:CDS 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.1:87144680-87144699 None:intergenic 45.0%
AGCTGAAGAGGACATGATAG+AGG + chr7.1:87144668-87144687 None:intergenic 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.1:87145303-87145322 MS.gene92216:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.1:87145087-87145106 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.1:87145283-87145302 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.1:87145415-87145434 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.1:87145408-87145427 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.1:87145401-87145420 MS.gene92216:CDS 45.0%
GCATCAGTTGCTCCAAACAA+AGG + chr7.1:87145335-87145354 None:intergenic 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.1:87145049-87145068 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.1:87144693-87144712 MS.gene92216:CDS 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.1:87144816-87144835 MS.gene92216:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.1:87145108-87145127 MS.gene92216:CDS 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.1:87145401-87145420 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.1:87145400-87145419 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.1:87144989-87145008 MS.gene92216:intron 45.0%
CAGATTGTACCGAAGAGGCA+CGG + chr7.1:87144705-87144724 None:intergenic 50.0%
GTTGGACTAAAGTCCATGGC+TGG - chr7.1:87144742-87144761 MS.gene92216:CDS 50.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.1:87145274-87145293 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.1:87145042-87145061 None:intergenic 50.0%
!! TCGTTGTTTTGTCGCGACCA+AGG - chr7.1:87145173-87145192 MS.gene92216:intron 50.0%
CACGCCAGATTGTACCGAAG+AGG + chr7.1:87144710-87144729 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.1:87145272-87145291 MS.gene92216:intron 55.0%
CGTGCCTCTTCGGTACAATC+TGG - chr7.1:87144703-87144722 MS.gene92216:CDS 55.0%
CTCTTCGGTACAATCTGGCG+TGG - chr7.1:87144708-87144727 MS.gene92216:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.1:87144758-87144777 None:intergenic 55.0%
TTCGGTACAATCTGGCGTGG+CGG - chr7.1:87144711-87144730 MS.gene92216:CDS 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.1:87145093-87145112 MS.gene92216:CDS 55.0%
GGCGTGGCGGCTCCATTCGT+TGG - chr7.1:87144724-87144743 MS.gene92216:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 87144658 87145487 87144658 ID=MS.gene92216
chr7.1 mRNA 87144658 87145487 87144658 ID=MS.gene92216.t1;Parent=MS.gene92216
chr7.1 exon 87145308 87145487 87145308 ID=MS.gene92216.t1.exon1;Parent=MS.gene92216.t1
chr7.1 CDS 87145308 87145487 87145308 ID=cds.MS.gene92216.t1;Parent=MS.gene92216.t1
chr7.1 exon 87145016 87145150 87145016 ID=MS.gene92216.t1.exon2;Parent=MS.gene92216.t1
chr7.1 CDS 87145016 87145150 87145016 ID=cds.MS.gene92216.t1;Parent=MS.gene92216.t1
chr7.1 exon 87144658 87144888 87144658 ID=MS.gene92216.t1.exon3;Parent=MS.gene92216.t1
chr7.1 CDS 87144658 87144888 87144658 ID=cds.MS.gene92216.t1;Parent=MS.gene92216.t1
Gene Sequence

>MS.gene92216

ATGGCTTCCTCTATCATGTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCTGGCGTGGCGGCTCCATTCGTTGGACTAAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACTTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAAAATTTATGACATGTCTGTAAACTGTTTTCATCTTATATTTTCATAAGCTCTTCAGGATAGTGTATGAAAACAGCTTATACGTACATAAGCACTTATAGATTCATTGGCAAAAGTACACAAGTTTGTTAACCCTTTTGGGCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCAAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGACCAAGGTCACAGTCACAAATTTGTACTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACTGATGCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene92216.t1

MASSIMSSSAVTTVNRASSVQSGVAAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDASQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY