AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80966


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80966.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 1.78e-124 348
MS.gene80966.t1 MTR_7g007200 94.152 171 10 0 11 181 11 181 3.33e-124 347
MS.gene80966.t1 MTR_7g007220 94.737 171 9 0 11 181 11 181 3.36e-124 347
MS.gene80966.t1 MTR_7g007120 94.706 170 9 0 12 181 12 181 7.25e-124 347
MS.gene80966.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 9.54e-124 346
MS.gene80966.t1 MTR_7g007230 92.941 170 12 0 12 181 12 181 5.30e-121 339
MS.gene80966.t1 MTR_6g018310 85.475 179 25 1 4 181 2 180 4.00e-115 324
MS.gene80966.t1 MTR_6g018300 84.916 179 23 2 4 181 2 177 2.84e-112 317
MS.gene80966.t1 MTR_7g007210 94.805 154 8 0 11 164 11 164 2.48e-110 313
MS.gene80966.t1 MTR_4g127870 50.641 156 73 3 28 180 15 169 1.09e-57 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80966.t1 AT1G67090 71.429 182 47 2 1 181 1 178 6.50e-94 271
MS.gene80966.t1 AT5G38430 70.879 182 48 2 1 181 1 178 6.15e-93 268
MS.gene80966.t1 AT5G38430 70.879 182 48 2 1 181 1 178 6.15e-93 268
MS.gene80966.t1 AT5G38410 70.330 182 49 2 1 181 1 178 1.30e-92 268
MS.gene80966.t1 AT5G38420 70.330 182 49 2 1 181 1 178 3.51e-92 266
MS.gene80966.t1 AT5G38410 68.449 187 49 3 1 181 1 183 2.53e-90 262
MS.gene80966.t1 AT5G38410 67.582 182 47 3 1 181 1 171 1.08e-85 249
MS.gene80966.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.01e-45 147

Find 55 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.266546 7.3:-90804514 MS.gene80966:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.3:+90804501 None:intergenic
TCCTTGTTGATCTTTGTAAC+TGG 0.307724 7.3:+90804875 None:intergenic
GGCGCGGTGGCTCCATTTGT+TGG 0.347642 7.3:-90804920 MS.gene80966:CDS
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.3:-90804492 MS.gene80966:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.3:-90804291 MS.gene80966:CDS
TTCTTTCGCCAATTGTTCTC+TGG 0.378466 7.3:+90804532 None:intergenic
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.3:-90804236 MS.gene80966:CDS
TCTTCCACCATTGCTTGTAA+TGG 0.401668 7.3:+90804844 None:intergenic
GCCAATTGTTCTCTGGTCAA+TGG 0.405140 7.3:+90804539 None:intergenic
ACCATTGACCAGAGAACAAT+TGG 0.409622 7.3:-90804540 MS.gene80966:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.3:-90804265 MS.gene80966:CDS
ATATAGGTGTGGCCTCCTAT+TGG 0.416991 7.3:-90804596 MS.gene80966:intron
GTTGGACTCAAGTCCATGGC+TGG 0.431505 7.3:-90804902 MS.gene80966:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435101 7.3:+90804581 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.3:+90804187 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.3:+90804237 None:intergenic
CGCGCCTCGTCGGTTCAATC+CGG 0.461844 7.3:-90804941 MS.gene80966:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.3:-90804184 MS.gene80966:CDS
CCTTGTTGATCTTTGTAACT+GGG 0.505692 7.3:+90804876 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.3:-90804505 MS.gene80966:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.3:+90804584 None:intergenic
ATTTGTTGGACTCAAGTCCA+TGG 0.520007 7.3:-90804906 MS.gene80966:CDS
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.3:-90804510 MS.gene80966:CDS
ATGGACTTGAGTCCAACAAA+TGG 0.526746 7.3:+90804908 None:intergenic
TCCACCATTGCTTGTAATGG+AGG 0.530318 7.3:+90804847 None:intergenic
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.3:+90804305 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.3:-90804313 MS.gene80966:CDS
AACTGTAGTTACAGCTGAAG+AGG 0.546956 7.3:+90804967 None:intergenic
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.3:-90804504 MS.gene80966:CDS
AACAAATGGAGCCACCGCGC+CGG 0.560979 7.3:+90804922 None:intergenic
TACAGTTAACCGCGCCTCGT+CGG 0.566785 7.3:-90804951 MS.gene80966:CDS
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.3:-90804509 MS.gene80966:CDS
CAATAAACTTAGTAGACTGC+AGG 0.568140 7.3:+90804112 None:intergenic
TAACGTCCATCATAGTATCC+TGG 0.569613 7.3:+90804295 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.3:-90804301 MS.gene80966:CDS
CGGATTGAACCGACGAGGCG+CGG 0.577823 7.3:+90804942 None:intergenic
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.3:+90804173 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.3:+90804314 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.3:+90804253 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.600961 7.3:+90804542 None:intergenic
ATTACCTCCATTACAAGCAA+TGG 0.601253 7.3:-90804851 MS.gene80966:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.3:-90804477 MS.gene80966:intron
ACCTCCATTACAAGCAATGG+TGG 0.613355 7.3:-90804848 MS.gene80966:CDS
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.3:+90804889 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.3:+90804546 None:intergenic
GTCGGTTCAATCCGGCGCGG+TGG 0.622925 7.3:-90804933 MS.gene80966:CDS
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.3:-90804828 MS.gene80966:intron
AGCTGAAGAGGAGATCATAG+AGG 0.655372 7.3:+90804979 None:intergenic
TGATACGGACAAAGGCATCA+GGG 0.656050 7.3:+90804188 None:intergenic
CCCAGTTACAAAGATCAACA+AGG 0.670594 7.3:-90804876 MS.gene80966:CDS
CGCGCCGGATTGAACCGACG+AGG 0.686777 7.3:+90804937 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.3:+90804180 None:intergenic
CTCGTCGGTTCAATCCGGCG+CGG 0.728671 7.3:-90804936 MS.gene80966:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.3:-90804282 MS.gene80966:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTTGTACATTAT+TGG - chr7.3:90804355-90804374 MS.gene80966:intron 15.0%
!!! ATAATGTACAAAGTTTTAAT+TGG + chr7.3:90804355-90804374 None:intergenic 15.0%
!! AATGTACAAAAGATTAAGTT+AGG + chr7.3:90804484-90804503 None:intergenic 20.0%
!! ATGTACAAAAGATTAAGTTA+GGG + chr7.3:90804483-90804502 None:intergenic 20.0%
!! TTTGTACATTACTACTATAT+AGG - chr7.3:90804494-90804513 MS.gene80966:CDS 20.0%
!!! GATTTCATGTTTTTTTCATA+AGG - chr7.3:90804400-90804419 MS.gene80966:intron 20.0%
! AATAACATTAATGGAGTCAT+TGG - chr7.3:90804436-90804455 MS.gene80966:intron 25.0%
! AGTAAATGAATATTAGACCT+CGG + chr7.3:90804649-90804668 None:intergenic 25.0%
! TGTACAAAAGATTAAGTTAG+GGG + chr7.3:90804482-90804501 None:intergenic 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.3:90804592-90804611 MS.gene80966:CDS 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.3:90804745-90804764 MS.gene80966:intron 25.0%
ACATTACTACTATATAGGTG+TGG - chr7.3:90804499-90804518 MS.gene80966:CDS 30.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.3:90804596-90804615 MS.gene80966:intron 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.3:90804597-90804616 MS.gene80966:intron 30.0%
! GGGCTTAATAAAATTTTGCA+TGG + chr7.3:90804462-90804481 None:intergenic 30.0%
!! ATATAATCGTTGTTTTGTCG+CGG - chr7.3:90804688-90804707 MS.gene80966:intron 30.0%
ATTACCTCCATTACAAGCAA+TGG - chr7.3:90804255-90804274 MS.gene80966:CDS 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.3:90804601-90804620 MS.gene80966:intron 35.0%
CCTTGTTGATCTTTGTAACT+GGG + chr7.3:90804233-90804252 None:intergenic 35.0%
CTTCAACACTTGAGAAGAAT+CGG + chr7.3:90804872-90804891 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.3:90804528-90804547 None:intergenic 35.0%
TCCTTGTTGATCTTTGTAAC+TGG + chr7.3:90804234-90804253 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.3:90804602-90804621 MS.gene80966:intron 35.0%
AACTGTAGTTACAGCTGAAG+AGG + chr7.3:90804142-90804161 None:intergenic 40.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.3:90804525-90804544 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.3:90804805-90804824 MS.gene80966:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.3:90804567-90804586 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.3:90804566-90804585 MS.gene80966:CDS 40.0%
ATGGACTTGAGTCCAACAAA+TGG + chr7.3:90804201-90804220 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.3:90804841-90804860 MS.gene80966:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.3:90804714-90804733 None:intergenic 40.0%
CCCAGTTACAAAGATCAACA+AGG - chr7.3:90804230-90804249 MS.gene80966:CDS 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.3:90804760-90804779 MS.gene80966:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.3:90804814-90804833 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.3:90804265-90804284 None:intergenic 40.0%
TTCTTTCGCCAATTGTTCTC+TGG + chr7.3:90804577-90804596 None:intergenic 40.0%
! ATTTGTTGGACTCAAGTCCA+TGG - chr7.3:90804200-90804219 MS.gene80966:CDS 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.3:90804870-90804889 MS.gene80966:CDS 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.3:90804815-90804834 MS.gene80966:intron 40.0%
ACCTCCATTACAAGCAATGG+TGG - chr7.3:90804258-90804277 MS.gene80966:CDS 45.0%
AGCTGAAGAGGAGATCATAG+AGG + chr7.3:90804130-90804149 None:intergenic 45.0%
ATATAGGTGTGGCCTCCTAT+TGG - chr7.3:90804510-90804529 MS.gene80966:CDS 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.3:90804824-90804843 MS.gene80966:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.3:90804608-90804627 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.3:90804804-90804823 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.3:90804936-90804955 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.3:90804929-90804948 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.3:90804856-90804875 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.3:90804922-90804941 MS.gene80966:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.3:90804570-90804589 None:intergenic 45.0%
TCCACCATTGCTTGTAATGG+AGG + chr7.3:90804262-90804281 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.3:90804278-90804297 MS.gene80966:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.3:90804629-90804648 MS.gene80966:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.3:90804922-90804941 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.3:90804921-90804940 None:intergenic 45.0%
!! TTAAATTTAAATAACATTAA+TGG - chr7.3:90804427-90804446 MS.gene80966:intron 5.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.3:90804795-90804814 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.3:90804563-90804582 None:intergenic 50.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.3:90804793-90804812 MS.gene80966:intron 55.0%
GTTGGACTCAAGTCCATGGC+TGG - chr7.3:90804204-90804223 MS.gene80966:CDS 55.0%
TACAGTTAACCGCGCCTCGT+CGG - chr7.3:90804155-90804174 MS.gene80966:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.3:90804220-90804239 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.3:90804614-90804633 MS.gene80966:intron 55.0%
!! TCGTTGTTTTGTCGCGGCCA+AGG - chr7.3:90804694-90804713 MS.gene80966:intron 55.0%
AACAAATGGAGCCACCGCGC+CGG + chr7.3:90804187-90804206 None:intergenic 60.0%
CGGATTGAACCGACGAGGCG+CGG + chr7.3:90804167-90804186 None:intergenic 65.0%
GGCGCGGTGGCTCCATTTGT+TGG - chr7.3:90804186-90804205 MS.gene80966:CDS 65.0%
!! CGCGCCTCGTCGGTTCAATC+CGG - chr7.3:90804165-90804184 MS.gene80966:CDS 65.0%
!! CTCGTCGGTTCAATCCGGCG+CGG - chr7.3:90804170-90804189 MS.gene80966:CDS 65.0%
CGCGCCGGATTGAACCGACG+AGG + chr7.3:90804172-90804191 None:intergenic 70.0%
! GTCGGTTCAATCCGGCGCGG+TGG - chr7.3:90804173-90804192 MS.gene80966:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 90804120 90805008 90804120 ID=MS.gene80966
chr7.3 mRNA 90804120 90805008 90804120 ID=MS.gene80966.t1;Parent=MS.gene80966
chr7.3 exon 90804829 90805008 90804829 ID=MS.gene80966.t1.exon1;Parent=MS.gene80966.t1
chr7.3 CDS 90804829 90805008 90804829 ID=cds.MS.gene80966.t1;Parent=MS.gene80966.t1
chr7.3 exon 90804478 90804612 90804478 ID=MS.gene80966.t1.exon2;Parent=MS.gene80966.t1
chr7.3 CDS 90804478 90804612 90804478 ID=cds.MS.gene80966.t1;Parent=MS.gene80966.t1
chr7.3 exon 90804120 90804350 90804120 ID=MS.gene80966.t1.exon3;Parent=MS.gene80966.t1
chr7.3 CDS 90804120 90804350 90804120 ID=cds.MS.gene80966.t1;Parent=MS.gene80966.t1
Gene Sequence

>MS.gene80966

ATGGCTGCCTCTATGATCTCCTCTTCAGCTGTAACTACAGTTAACCGCGCCTCGTCGGTTCAATCCGGCGCGGTGGCTCCATTTGTTGGACTCAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAGGACATTACCTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAATACTGCGCTAAGTCTAGTTTGAAATAACTTTTAGTTCAAATTTTCCAATTAAAACTTTGTACATTATTGGTTCATAGTATTTTAGTTGAAATGATTTCATGTTTTTTTCATAAGGTTACTTAAATTTAAATAACATTAATGGAGTCATTGGCCATGCAAAATTTTATTAAGCCCCTAACTTAATCTTTTGTACATTACTACTATATAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCGAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTCATTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGGCCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene80966.t1

MAASMISSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY