Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54468.t1 | XP_013447451.1 | 93.9 | 181 | 11 | 0 | 1 | 181 | 1 | 181 | 1.90E-94 | 355.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54468.t1 | P07689 | 86.0 | 179 | 25 | 0 | 3 | 181 | 2 | 180 | 1.9e-87 | 323.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54468.t1 | A0A072U6Q1 | 93.9 | 181 | 11 | 0 | 1 | 181 | 1 | 181 | 1.4e-94 | 355.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene54468 | 0.902061 | 1.42E-78 | -1.69E-46 |
| MS.gene049173 | MS.gene54468 | 0.88187 | 1.68E-70 | -1.69E-46 |
| MS.gene049217 | MS.gene54468 | 0.8743 | 7.52E-68 | -1.69E-46 |
| MS.gene049222 | MS.gene54468 | 0.866901 | 2.03E-65 | -1.69E-46 |
| MS.gene049232 | MS.gene54468 | 0.838429 | 2.89E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene54468 | 0.936324 | 2.08E-97 | -1.69E-46 |
| MS.gene049313 | MS.gene54468 | -0.800301 | 1.54E-48 | -1.69E-46 |
| MS.gene049320 | MS.gene54468 | 0.925977 | 8.82E-91 | -1.69E-46 |
| MS.gene04939 | MS.gene54468 | 0.82204 | 2.93E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene54468 | 0.846146 | 2.61E-59 | -1.69E-46 |
| MS.gene049633 | MS.gene54468 | 0.844674 | 6.53E-59 | -1.69E-46 |
| MS.gene049805 | MS.gene54468 | 0.822908 | 1.84E-53 | -1.69E-46 |
| MS.gene05010 | MS.gene54468 | 0.881922 | 1.60E-70 | -1.69E-46 |
| MS.gene050163 | MS.gene54468 | 0.910431 | 1.89E-82 | -1.69E-46 |
| MS.gene050165 | MS.gene54468 | 0.915862 | 3.55E-85 | -1.69E-46 |
| MS.gene050170 | MS.gene54468 | 0.8144 | 1.57E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene54468 | 0.815228 | 1.03E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene54468 | 0.846405 | 2.22E-59 | -1.69E-46 |
| MS.gene050333 | MS.gene54468 | 0.91723 | 6.83E-86 | -1.69E-46 |
| MS.gene050444 | MS.gene54468 | 0.854229 | 1.41E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene54468 | 0.850067 | 2.16E-60 | -1.69E-46 |
| MS.gene050504 | MS.gene54468 | 0.904871 | 7.80E-80 | -1.69E-46 |
| MS.gene050518 | MS.gene54468 | 0.898758 | 3.87E-77 | -1.69E-46 |
| MS.gene050577 | MS.gene54468 | 0.840487 | 8.44E-58 | -1.69E-46 |
| MS.gene050593 | MS.gene54468 | 0.808515 | 2.98E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene54468 | 0.919988 | 2.26E-87 | -1.69E-46 |
| MS.gene050662 | MS.gene54468 | 0.811491 | 6.82E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene54468 | 0.90016 | 9.67E-78 | -1.69E-46 |
| MS.gene050699 | MS.gene54468 | 0.909278 | 6.80E-82 | -1.69E-46 |
| MS.gene050747 | MS.gene54468 | 0.804302 | 2.31E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene54468 | 0.814171 | 1.76E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene54468 | 0.817095 | 3.94E-52 | -1.69E-46 |
| MS.gene050772 | MS.gene54468 | 0.899534 | 1.80E-77 | -1.69E-46 |
| MS.gene05080 | MS.gene54468 | 0.833147 | 6.30E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene54468 | 0.829519 | 4.92E-55 | -1.69E-46 |
| MS.gene050871 | MS.gene54468 | 0.82506 | 5.76E-54 | -1.69E-46 |
| MS.gene050876 | MS.gene54468 | 0.851262 | 9.95E-61 | -1.69E-46 |
| MS.gene050904 | MS.gene54468 | 0.808586 | 2.88E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene54468 | 0.900196 | 9.33E-78 | -1.69E-46 |
| MS.gene05100 | MS.gene54468 | 0.873553 | 1.34E-67 | -1.69E-46 |
| MS.gene051228 | MS.gene54468 | 0.872754 | 2.49E-67 | -1.69E-46 |
| MS.gene051317 | MS.gene54468 | 0.846451 | 2.15E-59 | -1.69E-46 |
| MS.gene051364 | MS.gene54468 | -0.808877 | 2.50E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene54468 | -0.819814 | 9.53E-53 | -1.69E-46 |
| MS.gene051434 | MS.gene54468 | 0.821499 | 3.91E-53 | -1.69E-46 |
| MS.gene051551 | MS.gene54468 | 0.859443 | 4.12E-63 | -1.69E-46 |
| MS.gene051666 | MS.gene54468 | 0.810865 | 9.32E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene54468 | 0.907693 | 3.85E-81 | -1.69E-46 |
| MS.gene051858 | MS.gene54468 | 0.843891 | 1.06E-58 | -1.69E-46 |
| MS.gene051874 | MS.gene54468 | 0.80043 | 1.45E-48 | -1.69E-46 |
| MS.gene051949 | MS.gene54468 | 0.898846 | 3.55E-77 | -1.69E-46 |
| MS.gene052164 | MS.gene54468 | 0.830148 | 3.46E-55 | -1.69E-46 |
| MS.gene052271 | MS.gene54468 | 0.890195 | 1.23E-73 | -1.69E-46 |
| MS.gene052379 | MS.gene54468 | 0.834711 | 2.56E-56 | -1.69E-46 |
| MS.gene052482 | MS.gene54468 | 0.815323 | 9.81E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene54468 | 0.822318 | 2.53E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene54468 | 0.815243 | 1.02E-51 | -1.69E-46 |
| MS.gene052506 | MS.gene54468 | 0.835661 | 1.47E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene54468 | 0.827584 | 1.44E-54 | -1.69E-46 |
| MS.gene052822 | MS.gene54468 | 0.862176 | 6.10E-64 | -1.69E-46 |
| MS.gene052823 | MS.gene54468 | 0.80024 | 1.58E-48 | -1.69E-46 |
| MS.gene052870 | MS.gene54468 | 0.819531 | 1.11E-52 | -1.69E-46 |
| MS.gene052872 | MS.gene54468 | 0.837461 | 5.13E-57 | -1.69E-46 |
| MS.gene052916 | MS.gene54468 | 0.881292 | 2.71E-70 | -1.69E-46 |
| MS.gene052966 | MS.gene54468 | 0.832752 | 7.90E-56 | -1.69E-46 |
| MS.gene053009 | MS.gene54468 | 0.827349 | 1.64E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene54468 | 0.806052 | 9.92E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene54468 | 0.832735 | 7.98E-56 | -1.69E-46 |
| MS.gene053207 | MS.gene54468 | 0.832852 | 7.46E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene54468 | 0.886237 | 4.07E-72 | -1.69E-46 |
| MS.gene053284 | MS.gene54468 | 0.808139 | 3.59E-50 | -1.69E-46 |
| MS.gene053286 | MS.gene54468 | 0.884358 | 2.05E-71 | -1.69E-46 |
| MS.gene053322 | MS.gene54468 | 0.8202 | 7.78E-53 | -1.69E-46 |
| MS.gene053561 | MS.gene54468 | -0.815858 | 7.45E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene54468 | 0.836373 | 9.72E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene54468 | 0.879729 | 9.82E-70 | -1.69E-46 |
| MS.gene053824 | MS.gene54468 | 0.841508 | 4.55E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene54468 | 0.873631 | 1.27E-67 | -1.69E-46 |
| MS.gene054595 | MS.gene54468 | 0.818003 | 2.46E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene54468 | 0.875218 | 3.67E-68 | -1.69E-46 |
| MS.gene054642 | MS.gene54468 | 0.9315 | 3.44E-94 | -1.69E-46 |
| MS.gene05470 | MS.gene54468 | 0.828304 | 9.68E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene54468 | 0.927352 | 1.32E-91 | -1.69E-46 |
| MS.gene054959 | MS.gene54468 | -0.812601 | 3.91E-51 | -1.69E-46 |
| MS.gene05505 | MS.gene54468 | 0.810423 | 1.16E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene54468 | -0.806692 | 7.27E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene54468 | 0.836258 | 1.04E-56 | -1.69E-46 |
| MS.gene055212 | MS.gene54468 | 0.823853 | 1.11E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene54468 | 0.926553 | 4.01E-91 | -1.69E-46 |
| MS.gene055397 | MS.gene54468 | 0.833439 | 5.33E-56 | -1.69E-46 |
| MS.gene055499 | MS.gene54468 | 0.817726 | 2.84E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene54468 | 0.895196 | 1.21E-75 | -1.69E-46 |
| MS.gene055577 | MS.gene54468 | 0.869779 | 2.40E-66 | -1.69E-46 |
| MS.gene055634 | MS.gene54468 | 0.863918 | 1.77E-64 | -1.69E-46 |
| MS.gene055636 | MS.gene54468 | 0.85642 | 3.26E-62 | -1.69E-46 |
| MS.gene055638 | MS.gene54468 | 0.877902 | 4.33E-69 | -1.69E-46 |
| MS.gene055647 | MS.gene54468 | 0.82891 | 6.91E-55 | -1.69E-46 |
| MS.gene055722 | MS.gene54468 | 0.87856 | 2.54E-69 | -1.69E-46 |
| MS.gene055748 | MS.gene54468 | 0.860011 | 2.78E-63 | -1.69E-46 |
| MS.gene055750 | MS.gene54468 | 0.847126 | 1.41E-59 | -1.69E-46 |
| MS.gene055751 | MS.gene54468 | 0.87293 | 2.18E-67 | -1.69E-46 |
| MS.gene055776 | MS.gene54468 | 0.896857 | 2.47E-76 | -1.69E-46 |
| MS.gene055849 | MS.gene54468 | 0.842855 | 2.00E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene54468 | 0.827949 | 1.18E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene54468 | 0.856822 | 2.48E-62 | -1.69E-46 |
| MS.gene055941 | MS.gene54468 | 0.848011 | 8.04E-60 | -1.69E-46 |
| MS.gene055942 | MS.gene54468 | -0.844624 | 6.74E-59 | -1.69E-46 |
| MS.gene055956 | MS.gene54468 | -0.850652 | 1.48E-60 | -1.69E-46 |
| MS.gene056046 | MS.gene54468 | 0.800987 | 1.11E-48 | -1.69E-46 |
| MS.gene056083 | MS.gene54468 | 0.839797 | 1.28E-57 | -1.69E-46 |
| MS.gene056374 | MS.gene54468 | 0.877331 | 6.85E-69 | -1.69E-46 |
| MS.gene056504 | MS.gene54468 | 0.879993 | 7.91E-70 | -1.69E-46 |
| MS.gene056510 | MS.gene54468 | 0.890677 | 7.93E-74 | -1.69E-46 |
| MS.gene056549 | MS.gene54468 | 0.8162 | 6.25E-52 | -1.69E-46 |
| MS.gene056657 | MS.gene54468 | 0.930097 | 2.69E-93 | -1.69E-46 |
| MS.gene056692 | MS.gene54468 | 0.85031 | 1.84E-60 | -1.69E-46 |
| MS.gene056761 | MS.gene54468 | -0.819713 | 1.01E-52 | -1.69E-46 |
| MS.gene056898 | MS.gene54468 | 0.830374 | 3.05E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene54468 | 0.835928 | 1.26E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene54468 | 0.865308 | 6.49E-65 | -1.69E-46 |
| MS.gene057246 | MS.gene54468 | 0.814033 | 1.89E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene54468 | 0.847238 | 1.31E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene54468 | 0.865196 | 7.04E-65 | -1.69E-46 |
| MS.gene057831 | MS.gene54468 | 0.825459 | 4.63E-54 | -1.69E-46 |
| MS.gene057857 | MS.gene54468 | 0.806085 | 9.76E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene54468 | 0.832373 | 9.81E-56 | -1.69E-46 |
| MS.gene058019 | MS.gene54468 | 0.817103 | 3.92E-52 | -1.69E-46 |
| MS.gene058334 | MS.gene54468 | -0.810372 | 1.19E-50 | -1.69E-46 |
| MS.gene058335 | MS.gene54468 | 0.804483 | 2.11E-49 | -1.69E-46 |
| MS.gene058338 | MS.gene54468 | 0.810665 | 1.03E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene54468 | 0.929102 | 1.12E-92 | -1.69E-46 |
| MS.gene058544 | MS.gene54468 | 0.855496 | 6.07E-62 | -1.69E-46 |
| MS.gene058719 | MS.gene54468 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene058723 | MS.gene54468 | 0.893392 | 6.56E-75 | -1.69E-46 |
| MS.gene058773 | MS.gene54468 | 0.897508 | 1.31E-76 | -1.69E-46 |
| MS.gene058838 | MS.gene54468 | 0.834862 | 2.35E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene54468 | 0.922688 | 7.10E-89 | -1.69E-46 |
| MS.gene059023 | MS.gene54468 | 0.867346 | 1.46E-65 | -1.69E-46 |
| MS.gene059029 | MS.gene54468 | 0.866994 | 1.90E-65 | -1.69E-46 |
| MS.gene059079 | MS.gene54468 | 0.824146 | 9.45E-54 | -1.69E-46 |
| MS.gene05912 | MS.gene54468 | 0.807361 | 5.25E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene54468 | 0.860402 | 2.12E-63 | -1.69E-46 |
| MS.gene05934 | MS.gene54468 | 0.807118 | 5.91E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene54468 | 0.935254 | 1.13E-96 | -1.69E-46 |
| MS.gene059718 | MS.gene54468 | 0.893576 | 5.53E-75 | -1.69E-46 |
| MS.gene059756 | MS.gene54468 | 0.857101 | 2.05E-62 | -1.69E-46 |
| MS.gene059801 | MS.gene54468 | 0.81865 | 1.75E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene54468 | 0.887934 | 9.22E-73 | -1.69E-46 |
| MS.gene059902 | MS.gene54468 | 0.835299 | 1.82E-56 | -1.69E-46 |
| MS.gene059960 | MS.gene54468 | 0.816004 | 6.91E-52 | -1.69E-46 |
| MS.gene060193 | MS.gene54468 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene060303 | MS.gene54468 | 0.800577 | 1.35E-48 | -1.69E-46 |
| MS.gene060336 | MS.gene54468 | -0.807565 | 4.75E-50 | -1.69E-46 |
| MS.gene060337 | MS.gene54468 | -0.866962 | 1.94E-65 | -1.69E-46 |
| MS.gene060341 | MS.gene54468 | 0.836075 | 1.16E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene54468 | 0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene060703 | MS.gene54468 | 0.903089 | 4.97E-79 | -1.69E-46 |
| MS.gene060727 | MS.gene54468 | 0.800595 | 1.34E-48 | -1.69E-46 |
| MS.gene060728 | MS.gene54468 | 0.820801 | 5.66E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene54468 | 0.876711 | 1.12E-68 | -1.69E-46 |
| MS.gene060778 | MS.gene54468 | 0.807571 | 4.74E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene54468 | 0.858778 | 6.52E-63 | -1.69E-46 |
| MS.gene060804 | MS.gene54468 | 0.801597 | 8.35E-49 | -1.69E-46 |
| MS.gene060818 | MS.gene54468 | 0.809211 | 2.12E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene54468 | 0.818621 | 1.78E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene54468 | 0.867125 | 1.72E-65 | -1.69E-46 |
| MS.gene061134 | MS.gene54468 | 0.806603 | 7.59E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene54468 | 0.882993 | 6.53E-71 | -1.69E-46 |
| MS.gene061200 | MS.gene54468 | 0.839833 | 1.25E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76905 | MS.gene54468 | PPI |
| MS.gene074566 | MS.gene54468 | PPI |
| MS.gene92216 | MS.gene54468 | PPI |
| MS.gene76908 | MS.gene54468 | PPI |
| MS.gene54468 | MS.gene92218 | PPI |
| MS.gene92217 | MS.gene54468 | PPI |
| MS.gene80971 | MS.gene54468 | PPI |
| MS.gene074248 | MS.gene54468 | PPI |
| MS.gene074249 | MS.gene54468 | PPI |
| MS.gene54468 | MS.gene074567 | PPI |
| MS.gene54465 | MS.gene54468 | PPI |
| MS.gene074247 | MS.gene54468 | PPI |
| MS.gene54468 | MS.gene074568 | PPI |
| MS.gene54468 | MS.gene80971 | PPI |
| MS.gene54468 | MS.gene92220 | PPI |
| MS.gene54468 | MS.gene074247 | PPI |
| MS.gene80966 | MS.gene54468 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54468.t1 | MTR_7g007120 | 94.737 | 171 | 9 | 0 | 11 | 181 | 11 | 181 | 1.78e-124 | 348 |
| MS.gene54468.t1 | MTR_7g007200 | 94.152 | 171 | 10 | 0 | 11 | 181 | 11 | 181 | 3.33e-124 | 347 |
| MS.gene54468.t1 | MTR_7g007220 | 94.737 | 171 | 9 | 0 | 11 | 181 | 11 | 181 | 3.36e-124 | 347 |
| MS.gene54468.t1 | MTR_7g007120 | 94.706 | 170 | 9 | 0 | 12 | 181 | 12 | 181 | 7.25e-124 | 347 |
| MS.gene54468.t1 | MTR_7g007120 | 94.152 | 171 | 10 | 0 | 11 | 181 | 11 | 181 | 9.54e-124 | 346 |
| MS.gene54468.t1 | MTR_7g007230 | 92.941 | 170 | 12 | 0 | 12 | 181 | 12 | 181 | 5.30e-121 | 339 |
| MS.gene54468.t1 | MTR_6g018310 | 85.475 | 179 | 25 | 1 | 4 | 181 | 2 | 180 | 4.00e-115 | 324 |
| MS.gene54468.t1 | MTR_6g018300 | 84.916 | 179 | 23 | 2 | 4 | 181 | 2 | 177 | 2.84e-112 | 317 |
| MS.gene54468.t1 | MTR_7g007210 | 94.805 | 154 | 8 | 0 | 11 | 164 | 11 | 164 | 2.48e-110 | 313 |
| MS.gene54468.t1 | MTR_4g127870 | 50.641 | 156 | 73 | 3 | 28 | 180 | 15 | 169 | 1.09e-57 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54468.t1 | AT1G67090 | 71.429 | 182 | 47 | 2 | 1 | 181 | 1 | 178 | 6.50e-94 | 271 |
| MS.gene54468.t1 | AT5G38430 | 70.879 | 182 | 48 | 2 | 1 | 181 | 1 | 178 | 6.15e-93 | 268 |
| MS.gene54468.t1 | AT5G38430 | 70.879 | 182 | 48 | 2 | 1 | 181 | 1 | 178 | 6.15e-93 | 268 |
| MS.gene54468.t1 | AT5G38410 | 70.330 | 182 | 49 | 2 | 1 | 181 | 1 | 178 | 1.30e-92 | 268 |
| MS.gene54468.t1 | AT5G38420 | 70.330 | 182 | 49 | 2 | 1 | 181 | 1 | 178 | 3.51e-92 | 266 |
| MS.gene54468.t1 | AT5G38410 | 68.449 | 187 | 49 | 3 | 1 | 181 | 1 | 183 | 2.53e-90 | 262 |
| MS.gene54468.t1 | AT5G38410 | 67.582 | 182 | 47 | 3 | 1 | 181 | 1 | 171 | 1.08e-85 | 249 |
| MS.gene54468.t1 | AT1G67090 | 65.217 | 115 | 35 | 2 | 1 | 114 | 1 | 111 | 1.01e-45 | 147 |
Find 56 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGTTGATTACCTTATA+AGG | 0.266546 | 7.4:-92284491 | MS.gene54468:CDS |
| CAACCCATCCCTTCCTTATA+AGG | 0.273690 | 7.4:+92284478 | None:intergenic |
| TCCTTGTTGATCTTTGTAAC+TGG | 0.307724 | 7.4:+92284852 | None:intergenic |
| GGCGCGGTGGCTCCATTTGT+TGG | 0.347642 | 7.4:-92284897 | MS.gene54468:CDS |
| ATATAGGTGTGGCCTCCTAT+TGG | 0.366439 | 7.4:-92284573 | MS.gene54468:intron |
| GAAGGGATGGGTTGCTTGCT+TGG | 0.368783 | 7.4:-92284469 | MS.gene54468:CDS |
| GATACTATGATGGACGTTAC+TGG | 0.371866 | 7.4:-92284257 | MS.gene54468:CDS |
| TTCTTTCGCCAATTGTTCTC+TGG | 0.378466 | 7.4:+92284509 | None:intergenic |
| TATAGGTGTGGCCTCCTATT+GGG | 0.392616 | 7.4:-92284572 | MS.gene54468:intron |
| CGATTCTTCTCAAGTGTTGA+AGG | 0.396980 | 7.4:-92284202 | MS.gene54468:CDS |
| TCTTCCACCATTGCTTGTAA+TGG | 0.401668 | 7.4:+92284821 | None:intergenic |
| GCCAATTGTTCTCTGGTCAA+TGG | 0.405140 | 7.4:+92284516 | None:intergenic |
| ACCATTGACCAGAGAACAAT+TGG | 0.409622 | 7.4:-92284517 | MS.gene54468:CDS |
| ATGTGGAAGTTGCCTTTGTT+TGG | 0.411503 | 7.4:-92284231 | MS.gene54468:CDS |
| TCAAACTTCTTCTTCCCAAT+AGG | 0.428544 | 7.4:+92284558 | None:intergenic |
| GTTGGACTCAAGTCCATGGC+TGG | 0.431505 | 7.4:-92284879 | MS.gene54468:CDS |
| ATGATACGGACAAAGGCATC+AGG | 0.443523 | 7.4:+92284153 | None:intergenic |
| CTTCAACACTTGAGAAGAAT+CGG | 0.461194 | 7.4:+92284203 | None:intergenic |
| CGCGCCTCGTCGGTTCAATC+CGG | 0.461844 | 7.4:-92284918 | MS.gene54468:CDS |
| GATGCCTTTGTCCGTATCAT+CGG | 0.484184 | 7.4:-92284150 | MS.gene54468:CDS |
| CTCAAAATTTAGTAGACTGC+AGG | 0.495678 | 7.4:+92284078 | None:intergenic |
| CCTTGTTGATCTTTGTAACT+GGG | 0.505692 | 7.4:+92284853 | None:intergenic |
| ATTACCTTATAAGGAAGGGA+TGG | 0.507588 | 7.4:-92284482 | MS.gene54468:CDS |
| ATTTGTTGGACTCAAGTCCA+TGG | 0.520007 | 7.4:-92284883 | MS.gene54468:CDS |
| AGTTGATTACCTTATAAGGA+AGG | 0.522099 | 7.4:-92284487 | MS.gene54468:CDS |
| ATGGACTTGAGTCCAACAAA+TGG | 0.526746 | 7.4:+92284885 | None:intergenic |
| TCCACCATTGCTTGTAATGG+AGG | 0.530318 | 7.4:+92284824 | None:intergenic |
| AACTTCTTCTTCCCAATAGG+AGG | 0.534518 | 7.4:+92284561 | None:intergenic |
| CATAGTATCCTGGTGACTTG+TGG | 0.542155 | 7.4:+92284271 | None:intergenic |
| TACAGTTAACCGCGCCTCGT+CGG | 0.543383 | 7.4:-92284928 | MS.gene54468:CDS |
| CGTGAGAACCACAAGTCACC+AGG | 0.544938 | 7.4:-92284279 | MS.gene54468:CDS |
| TTACCTTATAAGGAAGGGAT+GGG | 0.559428 | 7.4:-92284481 | MS.gene54468:CDS |
| AACAAATGGAGCCACCGCGC+CGG | 0.560979 | 7.4:+92284899 | None:intergenic |
| GTTGATTACCTTATAAGGAA+GGG | 0.567383 | 7.4:-92284486 | MS.gene54468:CDS |
| TAACGTCCATCATAGTATCC+TGG | 0.569613 | 7.4:+92284261 | None:intergenic |
| AAGTCACCAGGATACTATGA+TGG | 0.574009 | 7.4:-92284267 | MS.gene54468:CDS |
| CGGATTGAACCGACGAGGCG+CGG | 0.577823 | 7.4:+92284919 | None:intergenic |
| CGTTGTCGAATCCGATGATA+CGG | 0.579522 | 7.4:+92284139 | None:intergenic |
| CTGGTGACTTGTGGTTCTCA+CGG | 0.595328 | 7.4:+92284280 | None:intergenic |
| AACTGTAGTCACAGCTGAAG+AGG | 0.598151 | 7.4:+92284944 | None:intergenic |
| GAATCGGTTGCTCCAAACAA+AGG | 0.598394 | 7.4:+92284219 | None:intergenic |
| AATTGTTCTCTGGTCAATGG+TGG | 0.600961 | 7.4:+92284519 | None:intergenic |
| ATTACCTCCATTACAAGCAA+TGG | 0.601253 | 7.4:-92284828 | MS.gene54468:CDS |
| TTGCTTGGAATTTGAGACCG+AGG | 0.613015 | 7.4:-92284454 | MS.gene54468:intron |
| ACCTCCATTACAAGCAATGG+TGG | 0.613355 | 7.4:-92284825 | MS.gene54468:CDS |
| TGTAACTGGGAAGCCAGCCA+TGG | 0.615947 | 7.4:+92284866 | None:intergenic |
| GTTCTCTGGTCAATGGTGGA+AGG | 0.618328 | 7.4:+92284523 | None:intergenic |
| GTCGGTTCAATCCGGCGCGG+TGG | 0.622925 | 7.4:-92284910 | MS.gene54468:CDS |
| TGGAAGAGTAAACTGCATGC+AGG | 0.652563 | 7.4:-92284805 | MS.gene54468:intron |
| AGCTGAAGAGGAGATCATAG+AGG | 0.655372 | 7.4:+92284956 | None:intergenic |
| TGATACGGACAAAGGCATCA+GGG | 0.656050 | 7.4:+92284154 | None:intergenic |
| CCCAGTTACAAAGATCAACA+AGG | 0.670594 | 7.4:-92284853 | MS.gene54468:CDS |
| CGCGCCGGATTGAACCGACG+AGG | 0.686777 | 7.4:+92284914 | None:intergenic |
| GAATCCGATGATACGGACAA+AGG | 0.687909 | 7.4:+92284146 | None:intergenic |
| CTCGTCGGTTCAATCCGGCG+CGG | 0.728671 | 7.4:-92284913 | MS.gene54468:CDS |
| ATGGACGTTACTGGACAATG+TGG | 0.742197 | 7.4:-92284248 | MS.gene54468:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAACTTTGTACATTAT+TGG | - | chr7.4:92284321-92284340 | MS.gene54468:intron | 15.0% |
| !!! | ATAATGTACAAAGTTTTAAT+TGG | + | chr7.4:92284321-92284340 | None:intergenic | 15.0% |
| !!! | GATTTCATTTTTTTTTCATA+AGG | - | chr7.4:92284366-92284385 | MS.gene54468:intron | 15.0% |
| !! | AATAACATTAATAGAGTCAT+TGG | - | chr7.4:92284402-92284421 | MS.gene54468:intron | 20.0% |
| !! | AATGTACAAAAGATTAAGTT+AGG | + | chr7.4:92284450-92284469 | None:intergenic | 20.0% |
| !! | ATGTACAAAAGATTAAGTTA+GGG | + | chr7.4:92284449-92284468 | None:intergenic | 20.0% |
| !! | TTTGTACATTACTACTATAT+AGG | - | chr7.4:92284460-92284479 | MS.gene54468:CDS | 20.0% |
| !!! | CTCAAATGGATTTTAAATAA+AGG | + | chr7.4:92284699-92284718 | None:intergenic | 20.0% |
| ! | AGTAAATGAATATTAGACCT+CGG | + | chr7.4:92284615-92284634 | None:intergenic | 25.0% |
| ! | TGTACAAAAGATTAAGTTAG+GGG | + | chr7.4:92284448-92284467 | None:intergenic | 25.0% |
| !! | AAGAAGTTGATTACCTTATA+AGG | - | chr7.4:92284558-92284577 | MS.gene54468:CDS | 25.0% |
| !!! | AAAATCCATTTGAGTTTTGA+TGG | - | chr7.4:92284705-92284724 | MS.gene54468:intron | 25.0% |
| ACATTACTACTATATAGGTG+TGG | - | chr7.4:92284465-92284484 | MS.gene54468:CDS | 30.0% | |
| AGTTGATTACCTTATAAGGA+AGG | - | chr7.4:92284562-92284581 | MS.gene54468:CDS | 30.0% | |
| GTTAACCATCAAAACTCAAA+TGG | + | chr7.4:92284713-92284732 | None:intergenic | 30.0% | |
| GTTGATTACCTTATAAGGAA+GGG | - | chr7.4:92284563-92284582 | MS.gene54468:CDS | 30.0% | |
| ! | GGGCTTAATAAAATTTTGCA+TGG | + | chr7.4:92284428-92284447 | None:intergenic | 30.0% |
| ATTACCTCCATTACAAGCAA+TGG | - | chr7.4:92284221-92284240 | MS.gene54468:CDS | 35.0% | |
| ATTACCTTATAAGGAAGGGA+TGG | - | chr7.4:92284567-92284586 | MS.gene54468:CDS | 35.0% | |
| CCTTGTTGATCTTTGTAACT+GGG | + | chr7.4:92284199-92284218 | None:intergenic | 35.0% | |
| CTTCAACACTTGAGAAGAAT+CGG | + | chr7.4:92284849-92284868 | None:intergenic | 35.0% | |
| TCAAACTTCTTCTTCCCAAT+AGG | + | chr7.4:92284494-92284513 | None:intergenic | 35.0% | |
| TCCTTGTTGATCTTTGTAAC+TGG | + | chr7.4:92284200-92284219 | None:intergenic | 35.0% | |
| TTACCTTATAAGGAAGGGAT+GGG | - | chr7.4:92284568-92284587 | MS.gene54468:CDS | 35.0% | |
| AACTTCTTCTTCCCAATAGG+AGG | + | chr7.4:92284491-92284510 | None:intergenic | 40.0% | |
| AAGTCACCAGGATACTATGA+TGG | - | chr7.4:92284782-92284801 | MS.gene54468:intron | 40.0% | |
| AATTGTTCTCTGGTCAATGG+TGG | + | chr7.4:92284533-92284552 | None:intergenic | 40.0% | |
| ACCATTGACCAGAGAACAAT+TGG | - | chr7.4:92284532-92284551 | MS.gene54468:CDS | 40.0% | |
| ATGGACTTGAGTCCAACAAA+TGG | + | chr7.4:92284167-92284186 | None:intergenic | 40.0% | |
| ATGTGGAAGTTGCCTTTGTT+TGG | - | chr7.4:92284818-92284837 | MS.gene54468:CDS | 40.0% | |
| CCCAGTTACAAAGATCAACA+AGG | - | chr7.4:92284196-92284215 | MS.gene54468:CDS | 40.0% | |
| CTACTTGCTTACTTGCAGAA+AGG | - | chr7.4:92284737-92284756 | MS.gene54468:intron | 40.0% | |
| TAACGTCCATCATAGTATCC+TGG | + | chr7.4:92284791-92284810 | None:intergenic | 40.0% | |
| TCTTCCACCATTGCTTGTAA+TGG | + | chr7.4:92284231-92284250 | None:intergenic | 40.0% | |
| TTCTTTCGCCAATTGTTCTC+TGG | + | chr7.4:92284543-92284562 | None:intergenic | 40.0% | |
| ! | ATTTGTTGGACTCAAGTCCA+TGG | - | chr7.4:92284166-92284185 | MS.gene54468:CDS | 40.0% |
| !! | CGATTCTTCTCAAGTGTTGA+AGG | - | chr7.4:92284847-92284866 | MS.gene54468:CDS | 40.0% |
| !! | GATACTATGATGGACGTTAC+TGG | - | chr7.4:92284792-92284811 | MS.gene54468:intron | 40.0% |
| AACTGTAGTCACAGCTGAAG+AGG | + | chr7.4:92284108-92284127 | None:intergenic | 45.0% | |
| ACCTCCATTACAAGCAATGG+TGG | - | chr7.4:92284224-92284243 | MS.gene54468:CDS | 45.0% | |
| AGCTGAAGAGGAGATCATAG+AGG | + | chr7.4:92284096-92284115 | None:intergenic | 45.0% | |
| ATATAGGTGTGGCCTCCTAT+TGG | - | chr7.4:92284476-92284495 | MS.gene54468:CDS | 45.0% | |
| ATGGACGTTACTGGACAATG+TGG | - | chr7.4:92284801-92284820 | MS.gene54468:intron | 45.0% | |
| CAACCCATCCCTTCCTTATA+AGG | + | chr7.4:92284574-92284593 | None:intergenic | 45.0% | |
| CATAGTATCCTGGTGACTTG+TGG | + | chr7.4:92284781-92284800 | None:intergenic | 45.0% | |
| CGTTGTCGAATCCGATGATA+CGG | + | chr7.4:92284913-92284932 | None:intergenic | 45.0% | |
| GAATCCGATGATACGGACAA+AGG | + | chr7.4:92284906-92284925 | None:intergenic | 45.0% | |
| GAATCGGTTGCTCCAAACAA+AGG | + | chr7.4:92284833-92284852 | None:intergenic | 45.0% | |
| GATGCCTTTGTCCGTATCAT+CGG | - | chr7.4:92284899-92284918 | MS.gene54468:CDS | 45.0% | |
| GCCAATTGTTCTCTGGTCAA+TGG | + | chr7.4:92284536-92284555 | None:intergenic | 45.0% | |
| TATAGGTGTGGCCTCCTATT+GGG | - | chr7.4:92284477-92284496 | MS.gene54468:CDS | 45.0% | |
| TCCACCATTGCTTGTAATGG+AGG | + | chr7.4:92284228-92284247 | None:intergenic | 45.0% | |
| TGGAAGAGTAAACTGCATGC+AGG | - | chr7.4:92284244-92284263 | MS.gene54468:CDS | 45.0% | |
| ! | TTGCTTGGAATTTGAGACCG+AGG | - | chr7.4:92284595-92284614 | MS.gene54468:intron | 45.0% |
| ! | TTTTGTCACGACCCTGATTG+TGG | - | chr7.4:92284666-92284685 | MS.gene54468:intron | 45.0% |
| !! | ATGATACGGACAAAGGCATC+AGG | + | chr7.4:92284899-92284918 | None:intergenic | 45.0% |
| !! | TGATACGGACAAAGGCATCA+GGG | + | chr7.4:92284898-92284917 | None:intergenic | 45.0% |
| AAAGGTCCGTGACCACAATC+AGG | + | chr7.4:92284681-92284700 | None:intergenic | 50.0% | |
| AAGGTCCGTGACCACAATCA+GGG | + | chr7.4:92284680-92284699 | None:intergenic | 50.0% | |
| ! | CTGGTGACTTGTGGTTCTCA+CGG | + | chr7.4:92284772-92284791 | None:intergenic | 50.0% |
| ! | GTTCTCTGGTCAATGGTGGA+AGG | + | chr7.4:92284529-92284548 | None:intergenic | 50.0% |
| CACGACCCTGATTGTGGTCA+CGG | - | chr7.4:92284672-92284691 | MS.gene54468:intron | 55.0% | |
| CGTGAGAACCACAAGTCACC+AGG | - | chr7.4:92284770-92284789 | MS.gene54468:intron | 55.0% | |
| GTTGGACTCAAGTCCATGGC+TGG | - | chr7.4:92284170-92284189 | MS.gene54468:CDS | 55.0% | |
| TACAGTTAACCGCGCCTCGT+CGG | - | chr7.4:92284121-92284140 | MS.gene54468:CDS | 55.0% | |
| TGTAACTGGGAAGCCAGCCA+TGG | + | chr7.4:92284186-92284205 | None:intergenic | 55.0% | |
| !! | GAAGGGATGGGTTGCTTGCT+TGG | - | chr7.4:92284580-92284599 | MS.gene54468:intron | 55.0% |
| AACAAATGGAGCCACCGCGC+CGG | + | chr7.4:92284153-92284172 | None:intergenic | 60.0% | |
| CGGATTGAACCGACGAGGCG+CGG | + | chr7.4:92284133-92284152 | None:intergenic | 65.0% | |
| GGCGCGGTGGCTCCATTTGT+TGG | - | chr7.4:92284152-92284171 | MS.gene54468:CDS | 65.0% | |
| !! | CGCGCCTCGTCGGTTCAATC+CGG | - | chr7.4:92284131-92284150 | MS.gene54468:CDS | 65.0% |
| !! | CTCGTCGGTTCAATCCGGCG+CGG | - | chr7.4:92284136-92284155 | MS.gene54468:CDS | 65.0% |
| CGCGCCGGATTGAACCGACG+AGG | + | chr7.4:92284138-92284157 | None:intergenic | 70.0% | |
| ! | GTCGGTTCAATCCGGCGCGG+TGG | - | chr7.4:92284139-92284158 | MS.gene54468:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 92284086 | 92284985 | 92284086 | ID=MS.gene54468 |
| chr7.4 | mRNA | 92284086 | 92284985 | 92284086 | ID=MS.gene54468.t1;Parent=MS.gene54468 |
| chr7.4 | exon | 92284806 | 92284985 | 92284806 | ID=MS.gene54468.t1.exon1;Parent=MS.gene54468.t1 |
| chr7.4 | CDS | 92284806 | 92284985 | 92284806 | ID=cds.MS.gene54468.t1;Parent=MS.gene54468.t1 |
| chr7.4 | exon | 92284455 | 92284589 | 92284455 | ID=MS.gene54468.t1.exon2;Parent=MS.gene54468.t1 |
| chr7.4 | CDS | 92284455 | 92284589 | 92284455 | ID=cds.MS.gene54468.t1;Parent=MS.gene54468.t1 |
| chr7.4 | exon | 92284086 | 92284316 | 92284086 | ID=MS.gene54468.t1.exon3;Parent=MS.gene54468.t1 |
| chr7.4 | CDS | 92284086 | 92284316 | 92284086 | ID=cds.MS.gene54468.t1;Parent=MS.gene54468.t1 |
| Gene Sequence |
| Protein sequence |
