AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80971


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80971.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 1.99e-124 348
MS.gene80971.t1 MTR_7g007200 94.152 171 10 0 11 181 11 181 3.29e-124 347
MS.gene80971.t1 MTR_7g007220 94.737 171 9 0 11 181 11 181 3.80e-124 347
MS.gene80971.t1 MTR_7g007120 94.706 170 9 0 12 181 12 181 8.46e-124 346
MS.gene80971.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 1.08e-123 346
MS.gene80971.t1 MTR_7g007230 92.941 170 12 0 12 181 12 181 5.72e-121 339
MS.gene80971.t1 MTR_6g018310 84.916 179 26 1 4 181 2 180 1.72e-114 323
MS.gene80971.t1 MTR_6g018300 84.358 179 24 2 4 181 2 177 9.49e-112 316
MS.gene80971.t1 MTR_7g007210 94.805 154 8 0 11 164 11 164 2.51e-110 313
MS.gene80971.t1 MTR_4g127870 50.641 156 73 3 28 180 15 169 1.12e-57 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80971.t1 AT1G67090 71.429 182 47 2 1 181 1 178 1.08e-93 270
MS.gene80971.t1 AT5G38430 70.879 182 48 2 1 181 1 178 1.06e-92 268
MS.gene80971.t1 AT5G38430 70.879 182 48 2 1 181 1 178 1.06e-92 268
MS.gene80971.t1 AT5G38410 70.330 182 49 2 1 181 1 178 2.07e-92 267
MS.gene80971.t1 AT5G38420 70.330 182 49 2 1 181 1 178 6.85e-92 266
MS.gene80971.t1 AT5G38410 68.449 187 49 3 1 181 1 183 3.68e-90 261
MS.gene80971.t1 AT5G38410 67.582 182 47 3 1 181 1 171 1.28e-85 249
MS.gene80971.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.72e-45 146

Find 51 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.244592 7.3:-90844302 MS.gene80971:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.3:+90844289 None:intergenic
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.3:+90844604 None:intergenic
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.3:-90844280 MS.gene80971:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.3:-90844079 MS.gene80971:CDS
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.3:-90844024 MS.gene80971:CDS
ACCATTGACCAGAGAACAAT+TGG 0.397843 7.3:-90844328 MS.gene80971:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.3:-90844053 MS.gene80971:CDS
GCCAATTGTTCTCTGGTCAA+TGG 0.416317 7.3:+90844327 None:intergenic
TCTTCCACCATTGCTTGTAA+TGG 0.422658 7.3:+90844573 None:intergenic
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.3:-90844680 MS.gene80971:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435806 7.3:+90844369 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.3:+90843975 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.3:+90844025 None:intergenic
GGCGCGGTGGCTCCATTCGT+TGG 0.461846 7.3:-90844649 MS.gene80971:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.3:-90843972 MS.gene80971:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.3:+90844605 None:intergenic
CTCAAAATTTAGTAGACTGC+AGG 0.495678 7.3:+90843900 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.3:-90844293 MS.gene80971:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.3:+90844372 None:intergenic
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.3:-90844298 MS.gene80971:CDS
CGTGCCTCTTCGGTACAATC+CGG 0.524408 7.3:-90844670 MS.gene80971:CDS
ATTACTTCCATTACAAGCAA+TGG 0.539617 7.3:-90844580 MS.gene80971:CDS
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.3:+90844093 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.3:-90844101 MS.gene80971:CDS
GTTGGACTAAAGTCCATGGC+TGG 0.555609 7.3:-90844631 MS.gene80971:CDS
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.3:-90844292 MS.gene80971:CDS
AACGAATGGAGCCACCGCGC+CGG 0.560979 7.3:+90844651 None:intergenic
AGCTGAAGAGGACATGATAG+AGG 0.561007 7.3:+90844708 None:intergenic
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.3:-90844297 MS.gene80971:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.3:+90844083 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.3:-90844089 MS.gene80971:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.3:+90843961 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.3:+90844102 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.597051 7.3:+90844696 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.3:+90844041 None:intergenic
ATTCGTTGGACTAAAGTCCA+TGG 0.609669 7.3:-90844635 MS.gene80971:CDS
ACTTCCATTACAAGCAATGG+TGG 0.610706 7.3:-90844577 MS.gene80971:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.3:-90844265 MS.gene80971:intron
TTCGGTACAATCCGGCGCGG+TGG 0.614407 7.3:-90844662 MS.gene80971:CDS
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.3:+90844618 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.3:+90844334 None:intergenic
ATGGACTTTAGTCCAACGAA+TGG 0.623442 7.3:+90844637 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.637841 7.3:+90844330 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.641462 7.3:+90844671 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.3:-90844557 MS.gene80971:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.3:+90843976 None:intergenic
CGCGCCGGATTGTACCGAAG+AGG 0.661856 7.3:+90844666 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.3:+90843968 None:intergenic
CTCTTCGGTACAATCCGGCG+CGG 0.696951 7.3:-90844665 MS.gene80971:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.3:-90844070 MS.gene80971:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTAAAGTAATATTAGACCT+CGG + chr7.3:90844378-90844397 None:intergenic 25.0%
! ATAAGCACTTATAGATTCAT+TGG - chr7.3:90844181-90844200 MS.gene80971:intron 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.3:90844321-90844340 MS.gene80971:CDS 25.0%
!! TATATTTTCATAAGCTCTTC+AGG - chr7.3:90844130-90844149 MS.gene80971:intron 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.3:90844474-90844493 MS.gene80971:intron 25.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.3:90844325-90844344 MS.gene80971:CDS 30.0%
ATTACTTCCATTACAAGCAA+TGG - chr7.3:90844043-90844062 MS.gene80971:CDS 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.3:90844021-90844040 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.3:90844326-90844345 MS.gene80971:CDS 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.3:90844022-90844041 None:intergenic 30.0%
!!! ACAAGTTTGTTAACCCTTTT+GGG - chr7.3:90844213-90844232 MS.gene80971:intron 30.0%
ACACCTAAAATTGCCCAAAA+GGG + chr7.3:90844229-90844248 None:intergenic 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.3:90844330-90844349 MS.gene80971:CDS 35.0%
CTTCAACACTTGAGAAGAAT+CGG + chr7.3:90844601-90844620 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.3:90844257-90844276 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.3:90844331-90844350 MS.gene80971:CDS 35.0%
! TAACCCTTTTGGGCAATTTT+AGG - chr7.3:90844223-90844242 MS.gene80971:intron 35.0%
! TTCTTTTGCCAATTGTTCTC+TGG + chr7.3:90844306-90844325 None:intergenic 35.0%
!!! CACAAGTTTGTTAACCCTTT+TGG - chr7.3:90844212-90844231 MS.gene80971:intron 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.3:90844254-90844273 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.3:90844534-90844553 MS.gene80971:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.3:90844296-90844315 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.3:90844295-90844314 MS.gene80971:CDS 40.0%
ACTTCCATTACAAGCAATGG+TGG - chr7.3:90844046-90844065 MS.gene80971:CDS 40.0%
ATGGACTTTAGTCCAACGAA+TGG + chr7.3:90843989-90844008 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.3:90844570-90844589 MS.gene80971:CDS 40.0%
ATTCGTTGGACTAAAGTCCA+TGG - chr7.3:90843988-90844007 MS.gene80971:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.3:90844443-90844462 None:intergenic 40.0%
CACACCTAAAATTGCCCAAA+AGG + chr7.3:90844230-90844249 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.3:90844489-90844508 MS.gene80971:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.3:90844543-90844562 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.3:90844053-90844072 None:intergenic 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.3:90844599-90844618 MS.gene80971:CDS 40.0%
!! CTTTTGGGCAATTTTAGGTG+TGG - chr7.3:90844228-90844247 MS.gene80971:intron 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.3:90844544-90844563 MS.gene80971:intron 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.3:90843930-90843949 None:intergenic 45.0%
AGCTGAAGAGGACATGATAG+AGG + chr7.3:90843918-90843937 None:intergenic 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.3:90844553-90844572 MS.gene80971:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.3:90844337-90844356 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.3:90844533-90844552 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.3:90844665-90844684 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.3:90844658-90844677 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.3:90844585-90844604 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.3:90844651-90844670 MS.gene80971:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.3:90844299-90844318 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.3:90843943-90843962 MS.gene80971:CDS 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.3:90844066-90844085 MS.gene80971:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.3:90844358-90844377 MS.gene80971:CDS 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.3:90844651-90844670 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.3:90844650-90844669 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.3:90844239-90844258 MS.gene80971:intron 45.0%
GTTGGACTAAAGTCCATGGC+TGG - chr7.3:90843992-90844011 MS.gene80971:CDS 50.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.3:90844524-90844543 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.3:90844292-90844311 None:intergenic 50.0%
!! TCGTTGTTTTGTCGCGACCA+AGG - chr7.3:90844423-90844442 MS.gene80971:intron 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.3:90843955-90843974 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.3:90844522-90844541 MS.gene80971:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.3:90843953-90843972 MS.gene80971:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.3:90844008-90844027 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.3:90844343-90844362 MS.gene80971:CDS 55.0%
CTCTTCGGTACAATCCGGCG+CGG - chr7.3:90843958-90843977 MS.gene80971:CDS 60.0%
AACGAATGGAGCCACCGCGC+CGG + chr7.3:90843975-90843994 None:intergenic 65.0%
CGCGCCGGATTGTACCGAAG+AGG + chr7.3:90843960-90843979 None:intergenic 65.0%
TTCGGTACAATCCGGCGCGG+TGG - chr7.3:90843961-90843980 MS.gene80971:CDS 65.0%
GGCGCGGTGGCTCCATTCGT+TGG - chr7.3:90843974-90843993 MS.gene80971:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 90843908 90844737 90843908 ID=MS.gene80971
chr7.3 mRNA 90843908 90844737 90843908 ID=MS.gene80971.t1;Parent=MS.gene80971
chr7.3 exon 90844558 90844737 90844558 ID=MS.gene80971.t1.exon1;Parent=MS.gene80971.t1
chr7.3 CDS 90844558 90844737 90844558 ID=cds.MS.gene80971.t1;Parent=MS.gene80971.t1
chr7.3 exon 90844266 90844400 90844266 ID=MS.gene80971.t1.exon2;Parent=MS.gene80971.t1
chr7.3 CDS 90844266 90844400 90844266 ID=cds.MS.gene80971.t1;Parent=MS.gene80971.t1
chr7.3 exon 90843908 90844138 90843908 ID=MS.gene80971.t1.exon3;Parent=MS.gene80971.t1
chr7.3 CDS 90843908 90844138 90843908 ID=cds.MS.gene80971.t1;Parent=MS.gene80971.t1
Gene Sequence

>MS.gene80971

ATGGCTTCCTCTATCATGTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGCGCGGTGGCTCCATTCGTTGGACTAAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACTTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAAAATTTATGACATGTCTGTAAACTGTTTTCATCTTATATTTTCATAAGCTCTTCAGGATAGTGTATGAAAACAGCTTATACGTACATAAGCACTTATAGATTCATTGGCAAAAATACACAAGTTTGTTAACCCTTTTGGGCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCAAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGACCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene80971.t1

MASSIMSSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY