AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92218


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92218.t1 MTR_7g007120 95.322 171 8 0 11 181 11 181 6.93e-125 349
MS.gene92218.t1 MTR_7g007200 94.737 171 9 0 11 181 11 181 1.19e-124 348
MS.gene92218.t1 MTR_7g007220 95.322 171 8 0 11 181 11 181 1.67e-124 348
MS.gene92218.t1 MTR_7g007120 95.294 170 8 0 12 181 12 181 3.01e-124 347
MS.gene92218.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 3.55e-124 347
MS.gene92218.t1 MTR_7g007230 93.529 170 11 0 12 181 12 181 2.20e-121 340
MS.gene92218.t1 MTR_6g018310 84.916 179 26 1 4 181 2 180 1.68e-114 323
MS.gene92218.t1 MTR_6g018300 84.358 179 24 2 4 181 2 177 9.28e-112 316
MS.gene92218.t1 MTR_7g007210 95.455 154 7 0 11 164 11 164 1.02e-110 313
MS.gene92218.t1 MTR_4g127870 50.000 156 74 3 28 180 15 169 2.65e-57 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92218.t1 AT1G67090 70.879 182 48 2 1 181 1 178 2.64e-93 269
MS.gene92218.t1 AT5G38430 70.330 182 49 2 1 181 1 178 2.73e-92 266
MS.gene92218.t1 AT5G38430 70.330 182 49 2 1 181 1 178 2.73e-92 266
MS.gene92218.t1 AT5G38410 69.780 182 50 2 1 181 1 178 6.07e-92 266
MS.gene92218.t1 AT5G38420 69.780 182 50 2 1 181 1 178 1.54e-91 265
MS.gene92218.t1 AT5G38410 67.914 187 50 3 1 181 1 183 1.19e-89 260
MS.gene92218.t1 AT5G38410 67.033 182 48 3 1 181 1 171 4.38e-85 248
MS.gene92218.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.47e-45 147

Find 54 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.266546 7.1:-87118800 MS.gene92218:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.1:+87118787 None:intergenic
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.1:+87119156 None:intergenic
GGCGCGGTGGCTCCATTTGT+TGG 0.347642 7.1:-87119201 MS.gene92218:CDS
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.1:-87118778 MS.gene92218:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.1:-87118582 MS.gene92218:CDS
TTCTTTCGCCAATTGTTCTC+TGG 0.378466 7.1:+87118818 None:intergenic
TCTTCCACCATTGCTTGTAA+TGG 0.401668 7.1:+87119125 None:intergenic
GCCAATTGTTCTCTGGTCAA+TGG 0.405140 7.1:+87118825 None:intergenic
ACCATTGACCAGAGAACAAT+TGG 0.409622 7.1:-87118826 MS.gene92218:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.1:-87118556 MS.gene92218:CDS
CGATGCTTCTCAAGTGTTGA+AGG 0.414382 7.1:-87118527 MS.gene92218:CDS
ATATAGGTGTGGCCTCCTAT+TGG 0.416991 7.1:-87118882 MS.gene92218:intron
GTTGGACTCAAGTCCATGGC+TGG 0.431505 7.1:-87119183 MS.gene92218:CDS
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.1:-87119232 MS.gene92218:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435101 7.1:+87118867 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.1:+87118478 None:intergenic
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.1:-87118475 MS.gene92218:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.1:+87119157 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.1:-87118791 MS.gene92218:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.1:+87118870 None:intergenic
ATTTGTTGGACTCAAGTCCA+TGG 0.520007 7.1:-87119187 MS.gene92218:CDS
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.1:-87118796 MS.gene92218:CDS
CGTGCCTCTTCGGTACAATC+CGG 0.524408 7.1:-87119222 MS.gene92218:CDS
ATGGACTTGAGTCCAACAAA+TGG 0.526746 7.1:+87119189 None:intergenic
TCCACCATTGCTTGTAATGG+AGG 0.530318 7.1:+87119128 None:intergenic
AGCTGAAGAGGAAATTATAG+AGG 0.539718 7.1:+87119260 None:intergenic
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.1:+87118596 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.1:-87118604 MS.gene92218:CDS
CTTCAACACTTGAGAAGCAT+CGG 0.552465 7.1:+87118528 None:intergenic
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.1:-87118790 MS.gene92218:CDS
AACAAATGGAGCCACCGCGC+CGG 0.560979 7.1:+87119203 None:intergenic
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.1:-87118795 MS.gene92218:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.1:+87118586 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.1:-87118592 MS.gene92218:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.1:+87118464 None:intergenic
ACTCAAACTTAGTAAACTGC+AGG 0.582384 7.1:+87118403 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.589798 7.1:+87119248 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.1:+87118605 None:intergenic
GCATCGGTTGCTCCAAACAA+AGG 0.600691 7.1:+87118544 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.600961 7.1:+87118828 None:intergenic
ATTACCTCCATTACAAGCAA+TGG 0.604904 7.1:-87119132 MS.gene92218:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.1:-87118763 MS.gene92218:intron
ACCTCCATTACAAGCAATGG+TGG 0.613355 7.1:-87119129 MS.gene92218:CDS
TTCGGTACAATCCGGCGCGG+TGG 0.614407 7.1:-87119214 MS.gene92218:CDS
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.1:+87119170 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.1:+87118832 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.641462 7.1:+87119223 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.1:-87119109 MS.gene92218:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.1:+87118479 None:intergenic
CGCGCCGGATTGTACCGAAG+AGG 0.661856 7.1:+87119218 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.1:+87118471 None:intergenic
CTCTTCGGTACAATCCGGCG+CGG 0.696951 7.1:-87119217 MS.gene92218:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.1:-87118573 MS.gene92218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACATTAATAGAGTCAT+TGG - chr7.1:87118722-87118741 MS.gene92218:intron 20.0%
!! AATGTACAAAAGATTAAGTT+AGG + chr7.1:87118770-87118789 None:intergenic 20.0%
!! ATAATGTACAAAGTTTGAAT+TGG + chr7.1:87118641-87118660 None:intergenic 20.0%
!! ATGTACAAAAGATTAAGTTA+GGG + chr7.1:87118769-87118788 None:intergenic 20.0%
!! ATTCAAACTTTGTACATTAT+TGG - chr7.1:87118641-87118660 MS.gene92218:intron 20.0%
!! TTTGTACATTACTACTATAT+AGG - chr7.1:87118780-87118799 MS.gene92218:CDS 20.0%
!!! GATTTCATGTTTTTTACATA+AGG - chr7.1:87118686-87118705 MS.gene92218:intron 20.0%
! TGTACAAAAGATTAAGTTAG+GGG + chr7.1:87118768-87118787 None:intergenic 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.1:87118878-87118897 MS.gene92218:CDS 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.1:87119026-87119045 MS.gene92218:intron 25.0%
ACATTACTACTATATAGGTG+TGG - chr7.1:87118785-87118804 MS.gene92218:CDS 30.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.1:87118882-87118901 MS.gene92218:intron 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.1:87118524-87118543 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.1:87118883-87118902 MS.gene92218:intron 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.1:87118525-87118544 None:intergenic 30.0%
TTGCAAGTAATATTAGACCT+CGG + chr7.1:87118935-87118954 None:intergenic 30.0%
! GGGCTTAATAAAATTTTGCA+TGG + chr7.1:87118748-87118767 None:intergenic 30.0%
!! ATATAATCGTTGTTTTGTCG+CGG - chr7.1:87118969-87118988 MS.gene92218:intron 30.0%
AGCTGAAGAGGAAATTATAG+AGG + chr7.1:87118421-87118440 None:intergenic 35.0%
ATTACCTCCATTACAAGCAA+TGG - chr7.1:87118546-87118565 MS.gene92218:CDS 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.1:87118887-87118906 MS.gene92218:intron 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.1:87118814-87118833 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.1:87118888-87118907 MS.gene92218:intron 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.1:87118811-87118830 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.1:87119086-87119105 MS.gene92218:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.1:87118853-87118872 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.1:87118852-87118871 MS.gene92218:CDS 40.0%
ATGGACTTGAGTCCAACAAA+TGG + chr7.1:87118492-87118511 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.1:87119122-87119141 MS.gene92218:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.1:87118995-87119014 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.1:87119041-87119060 MS.gene92218:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.1:87119095-87119114 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.1:87118556-87118575 None:intergenic 40.0%
TTCTTTCGCCAATTGTTCTC+TGG + chr7.1:87118863-87118882 None:intergenic 40.0%
! ATTTGTTGGACTCAAGTCCA+TGG - chr7.1:87118491-87118510 MS.gene92218:CDS 40.0%
! CTTCAACACTTGAGAAGCAT+CGG + chr7.1:87119153-87119172 None:intergenic 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.1:87119096-87119115 MS.gene92218:intron 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.1:87118433-87118452 None:intergenic 45.0%
ACCTCCATTACAAGCAATGG+TGG - chr7.1:87118549-87118568 MS.gene92218:CDS 45.0%
ATATAGGTGTGGCCTCCTAT+TGG - chr7.1:87118796-87118815 MS.gene92218:CDS 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.1:87119105-87119124 MS.gene92218:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.1:87118894-87118913 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.1:87119085-87119104 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.1:87119217-87119236 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.1:87119210-87119229 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.1:87119203-87119222 MS.gene92218:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.1:87118856-87118875 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.1:87118446-87118465 MS.gene92218:CDS 45.0%
TCCACCATTGCTTGTAATGG+AGG + chr7.1:87118553-87118572 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.1:87118569-87118588 MS.gene92218:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.1:87118915-87118934 MS.gene92218:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.1:87119203-87119222 None:intergenic 45.0%
!! CGATGCTTCTCAAGTGTTGA+AGG - chr7.1:87119151-87119170 MS.gene92218:CDS 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.1:87119202-87119221 None:intergenic 45.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.1:87119076-87119095 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.1:87118849-87118868 None:intergenic 50.0%
!! GCATCGGTTGCTCCAAACAA+AGG + chr7.1:87119137-87119156 None:intergenic 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.1:87118458-87118477 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.1:87119074-87119093 MS.gene92218:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.1:87118456-87118475 MS.gene92218:CDS 55.0%
GTTGGACTCAAGTCCATGGC+TGG - chr7.1:87118495-87118514 MS.gene92218:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.1:87118511-87118530 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.1:87118900-87118919 MS.gene92218:intron 55.0%
!! TCGTTGTTTTGTCGCGGCCA+AGG - chr7.1:87118975-87118994 MS.gene92218:intron 55.0%
AACAAATGGAGCCACCGCGC+CGG + chr7.1:87118478-87118497 None:intergenic 60.0%
CTCTTCGGTACAATCCGGCG+CGG - chr7.1:87118461-87118480 MS.gene92218:CDS 60.0%
CGCGCCGGATTGTACCGAAG+AGG + chr7.1:87118463-87118482 None:intergenic 65.0%
GGCGCGGTGGCTCCATTTGT+TGG - chr7.1:87118477-87118496 MS.gene92218:CDS 65.0%
TTCGGTACAATCCGGCGCGG+TGG - chr7.1:87118464-87118483 MS.gene92218:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 87118411 87119289 87118411 ID=MS.gene92218
chr7.1 mRNA 87118411 87119289 87118411 ID=MS.gene92218.t1;Parent=MS.gene92218
chr7.1 exon 87119110 87119289 87119110 ID=MS.gene92218.t1.exon1;Parent=MS.gene92218.t1
chr7.1 CDS 87119110 87119289 87119110 ID=cds.MS.gene92218.t1;Parent=MS.gene92218.t1
chr7.1 exon 87118764 87118898 87118764 ID=MS.gene92218.t1.exon2;Parent=MS.gene92218.t1
chr7.1 CDS 87118764 87118898 87118764 ID=cds.MS.gene92218.t1;Parent=MS.gene92218.t1
chr7.1 exon 87118411 87118641 87118411 ID=MS.gene92218.t1.exon3;Parent=MS.gene92218.t1
chr7.1 CDS 87118411 87118641 87118411 ID=cds.MS.gene92218.t1;Parent=MS.gene92218.t1
Gene Sequence

>MS.gene92218

ATGGCTTCCTCTATAATTTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGCGCGGTGGCTCCATTTGTTGGACTCAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACCTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAATACTAAGTCTAGTTTGAAATAACTTTTAGTTCAAGTTTTCCAATTCAAACTTTGTACATTATTGGTTCATAGTATTTTAGTTGAAATGATTTCATGTTTTTTACATAAGGTTACTTTAATTTAAATAACATTAATAGAGTCATTGGCCATGCAAAATTTTATTAAGCCCCTAACTTAATCTTTTGTACATTACTACTATATAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCGAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGGCCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATGCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTTTACTAA

Protein sequence

>MS.gene92218.t1

MASSIISSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDASQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY