AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92220.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 1.78e-124 348
MS.gene92220.t1 MTR_7g007200 94.152 171 10 0 11 181 11 181 3.33e-124 347
MS.gene92220.t1 MTR_7g007220 94.737 171 9 0 11 181 11 181 3.36e-124 347
MS.gene92220.t1 MTR_7g007120 94.706 170 9 0 12 181 12 181 7.25e-124 347
MS.gene92220.t1 MTR_7g007120 94.152 171 10 0 11 181 11 181 9.54e-124 346
MS.gene92220.t1 MTR_7g007230 92.941 170 12 0 12 181 12 181 5.30e-121 339
MS.gene92220.t1 MTR_6g018310 85.475 179 25 1 4 181 2 180 4.00e-115 324
MS.gene92220.t1 MTR_6g018300 84.916 179 23 2 4 181 2 177 2.84e-112 317
MS.gene92220.t1 MTR_7g007210 94.805 154 8 0 11 164 11 164 2.48e-110 313
MS.gene92220.t1 MTR_4g127870 50.641 156 73 3 28 180 15 169 1.09e-57 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92220.t1 AT1G67090 71.429 182 47 2 1 181 1 178 6.50e-94 271
MS.gene92220.t1 AT5G38430 70.879 182 48 2 1 181 1 178 6.15e-93 268
MS.gene92220.t1 AT5G38430 70.879 182 48 2 1 181 1 178 6.15e-93 268
MS.gene92220.t1 AT5G38410 70.330 182 49 2 1 181 1 178 1.30e-92 268
MS.gene92220.t1 AT5G38420 70.330 182 49 2 1 181 1 178 3.51e-92 266
MS.gene92220.t1 AT5G38410 68.449 187 49 3 1 181 1 183 2.53e-90 262
MS.gene92220.t1 AT5G38410 67.582 182 47 3 1 181 1 171 1.08e-85 249
MS.gene92220.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.01e-45 147

Find 55 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.266546 7.1:-87111458 MS.gene92220:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.1:+87111445 None:intergenic
TCCTTGTTGATCTTTGTAAC+TGG 0.307724 7.1:+87111807 None:intergenic
GGCGCGGTGGCTCCATTTGT+TGG 0.347642 7.1:-87111852 MS.gene92220:CDS
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.1:-87111436 MS.gene92220:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.1:-87111235 MS.gene92220:CDS
TTCTTTCGCCAATTGTTCTC+TGG 0.378466 7.1:+87111476 None:intergenic
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.1:-87111180 MS.gene92220:CDS
GCCAATTGTTCTCTGGTCAA+TGG 0.405140 7.1:+87111483 None:intergenic
ACCATTGACCAGAGAACAAT+TGG 0.409622 7.1:-87111484 MS.gene92220:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.1:-87111209 MS.gene92220:CDS
ATATAGGTGTGGCCTCCTAT+TGG 0.416991 7.1:-87111540 MS.gene92220:intron
GTTGGACTCAAGTCCATGGC+TGG 0.431505 7.1:-87111834 MS.gene92220:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435101 7.1:+87111525 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.1:+87111131 None:intergenic
TCTTCCACCATTGCTTGTGA+TGG 0.455939 7.1:+87111776 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.1:+87111181 None:intergenic
CGCGCCTCGTCGGTTCAATC+CGG 0.461844 7.1:-87111873 MS.gene92220:CDS
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.1:-87111128 MS.gene92220:CDS
TCCACCATTGCTTGTGATGG+AGG 0.503381 7.1:+87111779 None:intergenic
CCTTGTTGATCTTTGTAACT+GGG 0.505692 7.1:+87111808 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.1:-87111449 MS.gene92220:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.1:+87111528 None:intergenic
ATTTGTTGGACTCAAGTCCA+TGG 0.520007 7.1:-87111838 MS.gene92220:CDS
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.1:-87111454 MS.gene92220:CDS
ATGGACTTGAGTCCAACAAA+TGG 0.526746 7.1:+87111840 None:intergenic
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.1:+87111249 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.1:-87111257 MS.gene92220:CDS
AACTGTAGTTACAGCTGAAG+AGG 0.546956 7.1:+87111899 None:intergenic
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.1:-87111448 MS.gene92220:CDS
AACAAATGGAGCCACCGCGC+CGG 0.560979 7.1:+87111854 None:intergenic
TACAGTTAACCGCGCCTCGT+CGG 0.566785 7.1:-87111883 MS.gene92220:CDS
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.1:-87111453 MS.gene92220:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.1:+87111239 None:intergenic
CAATGAACTTAGTAGACTGC+AGG 0.571658 7.1:+87111056 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.1:-87111245 MS.gene92220:CDS
CGGATTGAACCGACGAGGCG+CGG 0.577823 7.1:+87111874 None:intergenic
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.1:+87111117 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.1:+87111258 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.1:+87111197 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.600961 7.1:+87111486 None:intergenic
ACCTCCATCACAAGCAATGG+TGG 0.602188 7.1:-87111780 MS.gene92220:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.1:-87111421 MS.gene92220:intron
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.1:+87111821 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.1:+87111490 None:intergenic
GTCGGTTCAATCCGGCGCGG+TGG 0.622925 7.1:-87111865 MS.gene92220:CDS
ATTACCTCCATCACAAGCAA+TGG 0.623970 7.1:-87111783 MS.gene92220:CDS
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.1:-87111760 MS.gene92220:intron
AGCTGAAGAGGAGATCATAG+AGG 0.655372 7.1:+87111911 None:intergenic
TGATACGGACAAAGGCATCA+GGG 0.656050 7.1:+87111132 None:intergenic
CCCAGTTACAAAGATCAACA+AGG 0.670594 7.1:-87111808 MS.gene92220:CDS
CGCGCCGGATTGAACCGACG+AGG 0.686777 7.1:+87111869 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.1:+87111124 None:intergenic
CTCGTCGGTTCAATCCGGCG+CGG 0.728671 7.1:-87111868 MS.gene92220:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.1:-87111226 MS.gene92220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTATGAATAAGTTTTAAT+TGG + chr7.1:87111299-87111318 None:intergenic 15.0%
!! AATAACATTAATAGAGTCAT+TGG - chr7.1:87111368-87111387 MS.gene92220:intron 20.0%
!! AATGTACAAAAGATTAAGTT+AGG + chr7.1:87111416-87111435 None:intergenic 20.0%
!! ATGTACAAAAGATTAAGTTA+GGG + chr7.1:87111415-87111434 None:intergenic 20.0%
!! TTTGTACATTACTACTATAT+AGG - chr7.1:87111426-87111445 MS.gene92220:CDS 20.0%
!!! GATTTCATGTTTTTTTCATA+AGG - chr7.1:87111332-87111351 MS.gene92220:intron 20.0%
! AGTAAAGTAATATTAGACCT+CGG + chr7.1:87111581-87111600 None:intergenic 25.0%
! TGTACAAAAGATTAAGTTAG+GGG + chr7.1:87111414-87111433 None:intergenic 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.1:87111524-87111543 MS.gene92220:CDS 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.1:87111677-87111696 MS.gene92220:intron 25.0%
ACATTACTACTATATAGGTG+TGG - chr7.1:87111431-87111450 MS.gene92220:CDS 30.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.1:87111528-87111547 MS.gene92220:CDS 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.1:87111529-87111548 MS.gene92220:CDS 30.0%
! GGGCTTAATAAAATTTTGCA+TGG + chr7.1:87111394-87111413 None:intergenic 30.0%
!! ATATAATCGTTGTTTTGTCG+CGG - chr7.1:87111620-87111639 MS.gene92220:intron 30.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.1:87111533-87111552 MS.gene92220:CDS 35.0%
CCTTGTTGATCTTTGTAACT+GGG + chr7.1:87111177-87111196 None:intergenic 35.0%
CTTCAACACTTGAGAAGAAT+CGG + chr7.1:87111804-87111823 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.1:87111460-87111479 None:intergenic 35.0%
TCCTTGTTGATCTTTGTAAC+TGG + chr7.1:87111178-87111197 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.1:87111534-87111553 MS.gene92220:CDS 35.0%
AACTGTAGTTACAGCTGAAG+AGG + chr7.1:87111086-87111105 None:intergenic 40.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.1:87111457-87111476 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.1:87111737-87111756 MS.gene92220:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.1:87111499-87111518 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.1:87111498-87111517 MS.gene92220:CDS 40.0%
ATGGACTTGAGTCCAACAAA+TGG + chr7.1:87111145-87111164 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.1:87111773-87111792 MS.gene92220:CDS 40.0%
ATTACCTCCATCACAAGCAA+TGG - chr7.1:87111199-87111218 MS.gene92220:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.1:87111646-87111665 None:intergenic 40.0%
CCCAGTTACAAAGATCAACA+AGG - chr7.1:87111174-87111193 MS.gene92220:CDS 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.1:87111692-87111711 MS.gene92220:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.1:87111746-87111765 None:intergenic 40.0%
TTCTTTCGCCAATTGTTCTC+TGG + chr7.1:87111509-87111528 None:intergenic 40.0%
! ATTTGTTGGACTCAAGTCCA+TGG - chr7.1:87111144-87111163 MS.gene92220:CDS 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.1:87111802-87111821 MS.gene92220:CDS 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.1:87111747-87111766 MS.gene92220:intron 40.0%
AGCTGAAGAGGAGATCATAG+AGG + chr7.1:87111074-87111093 None:intergenic 45.0%
ATATAGGTGTGGCCTCCTAT+TGG - chr7.1:87111442-87111461 MS.gene92220:CDS 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.1:87111756-87111775 MS.gene92220:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.1:87111540-87111559 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.1:87111736-87111755 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.1:87111868-87111887 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.1:87111861-87111880 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.1:87111788-87111807 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.1:87111854-87111873 MS.gene92220:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.1:87111502-87111521 None:intergenic 45.0%
TCTTCCACCATTGCTTGTGA+TGG + chr7.1:87111209-87111228 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.1:87111222-87111241 MS.gene92220:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.1:87111561-87111580 MS.gene92220:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.1:87111854-87111873 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.1:87111853-87111872 None:intergenic 45.0%
ACCTCCATCACAAGCAATGG+TGG - chr7.1:87111202-87111221 MS.gene92220:CDS 50.0%
TCCACCATTGCTTGTGATGG+AGG + chr7.1:87111206-87111225 None:intergenic 50.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.1:87111727-87111746 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.1:87111495-87111514 None:intergenic 50.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.1:87111725-87111744 MS.gene92220:intron 55.0%
GTTGGACTCAAGTCCATGGC+TGG - chr7.1:87111148-87111167 MS.gene92220:CDS 55.0%
TACAGTTAACCGCGCCTCGT+CGG - chr7.1:87111099-87111118 MS.gene92220:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.1:87111164-87111183 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.1:87111546-87111565 MS.gene92220:intron 55.0%
!! TCGTTGTTTTGTCGCGGCCA+AGG - chr7.1:87111626-87111645 MS.gene92220:intron 55.0%
AACAAATGGAGCCACCGCGC+CGG + chr7.1:87111131-87111150 None:intergenic 60.0%
CGGATTGAACCGACGAGGCG+CGG + chr7.1:87111111-87111130 None:intergenic 65.0%
GGCGCGGTGGCTCCATTTGT+TGG - chr7.1:87111130-87111149 MS.gene92220:CDS 65.0%
!! CGCGCCTCGTCGGTTCAATC+CGG - chr7.1:87111109-87111128 MS.gene92220:CDS 65.0%
!! CTCGTCGGTTCAATCCGGCG+CGG - chr7.1:87111114-87111133 MS.gene92220:CDS 65.0%
CGCGCCGGATTGAACCGACG+AGG + chr7.1:87111116-87111135 None:intergenic 70.0%
! GTCGGTTCAATCCGGCGCGG+TGG - chr7.1:87111117-87111136 MS.gene92220:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 87111064 87111940 87111064 ID=MS.gene92220
chr7.1 mRNA 87111064 87111940 87111064 ID=MS.gene92220.t1;Parent=MS.gene92220
chr7.1 exon 87111761 87111940 87111761 ID=MS.gene92220.t1.exon1;Parent=MS.gene92220.t1
chr7.1 CDS 87111761 87111940 87111761 ID=cds.MS.gene92220.t1;Parent=MS.gene92220.t1
chr7.1 exon 87111422 87111556 87111422 ID=MS.gene92220.t1.exon2;Parent=MS.gene92220.t1
chr7.1 CDS 87111422 87111556 87111422 ID=cds.MS.gene92220.t1;Parent=MS.gene92220.t1
chr7.1 exon 87111064 87111294 87111064 ID=MS.gene92220.t1.exon3;Parent=MS.gene92220.t1
chr7.1 CDS 87111064 87111294 87111064 ID=cds.MS.gene92220.t1;Parent=MS.gene92220.t1
Gene Sequence

>MS.gene92220

ATGGCTGCCTCTATGATCTCCTCTTCAGCTGTAACTACAGTTAACCGCGCCTCGTCGGTTCAATCCGGCGCGGTGGCTCCATTTGTTGGACTCAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAGGACATTACCTCCATCACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAATACTGCGCTAAGTCTAGTTTGAAATAACTTTTAGTTCAAGTTTTCCAATTAAAACTTATTCATAGTATTTTAGTTGAAATGATTTCATGTTTTTTTCATAAGGTTACTTAAATTTAAATAACATTAATAGAGTCATTGGCCATGCAAAATTTTATTAAGCCCCTAACTTAATCTTTTGTACATTACTACTATATAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCGAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGGCCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene92220.t1

MAASMISSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY