AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54465


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54465.t1 MTR_7g007120 95.322 171 8 0 11 181 11 181 6.21e-125 349
MS.gene54465.t1 MTR_7g007200 94.737 171 9 0 11 181 11 181 1.14e-124 348
MS.gene54465.t1 MTR_7g007220 95.322 171 8 0 11 181 11 181 1.16e-124 348
MS.gene54465.t1 MTR_7g007120 95.294 170 8 0 12 181 12 181 2.50e-124 348
MS.gene54465.t1 MTR_7g007120 94.737 171 9 0 11 181 11 181 3.22e-124 347
MS.gene54465.t1 MTR_7g007230 93.529 170 11 0 12 181 12 181 1.85e-121 340
MS.gene54465.t1 MTR_6g018310 84.358 179 27 1 4 181 2 180 3.79e-114 322
MS.gene54465.t1 MTR_6g018300 83.799 179 25 2 4 181 2 177 2.57e-111 315
MS.gene54465.t1 MTR_7g007210 95.455 154 7 0 11 164 11 164 8.47e-111 314
MS.gene54465.t1 MTR_4g127870 50.000 156 74 3 28 180 15 169 2.65e-57 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54465.t1 AT1G67090 70.879 182 48 2 1 181 1 178 2.61e-93 269
MS.gene54465.t1 AT5G38430 70.330 182 49 2 1 181 1 178 2.53e-92 267
MS.gene54465.t1 AT5G38430 70.330 182 49 2 1 181 1 178 2.53e-92 267
MS.gene54465.t1 AT5G38410 69.780 182 50 2 1 181 1 178 5.27e-92 266
MS.gene54465.t1 AT5G38420 69.780 182 50 2 1 181 1 178 1.58e-91 265
MS.gene54465.t1 AT5G38410 67.914 187 50 3 1 181 1 183 1.02e-89 260
MS.gene54465.t1 AT5G38410 67.033 182 48 3 1 181 1 171 4.33e-85 248
MS.gene54465.t1 AT1G67090 65.217 115 35 2 1 114 1 111 1.64e-45 147

Find 50 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGTTGATTACCTTATA+AGG 0.244592 7.4:-92304964 MS.gene54465:CDS
CAACCCATCCCTTCCTTATA+AGG 0.273690 7.4:+92304951 None:intergenic
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.4:+92305266 None:intergenic
GAAGGGATGGGTTGCTTGCT+TGG 0.368783 7.4:-92304942 MS.gene54465:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.4:-92304741 MS.gene54465:CDS
ACCATTGACCAGAGAACAAT+TGG 0.397843 7.4:-92304990 MS.gene54465:CDS
TGATGCTTCTCAAGTGTTGA+AGG 0.410018 7.4:-92304686 MS.gene54465:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.4:-92304715 MS.gene54465:CDS
GCCAATTGTTCTCTGGTCAA+TGG 0.416317 7.4:+92304989 None:intergenic
TCTTCCACCATTGCTTGTAA+TGG 0.422658 7.4:+92305235 None:intergenic
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.4:-92305342 MS.gene54465:CDS
TCAAACTTCTTCTTGCCAAT+AGG 0.435806 7.4:+92305031 None:intergenic
ATGATACGGACAAAGGCATC+AGG 0.443523 7.4:+92304637 None:intergenic
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.4:-92304634 MS.gene54465:CDS
GGTGCGGTGGCTCCATTCGT+TGG 0.494503 7.4:-92305311 MS.gene54465:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.4:+92305267 None:intergenic
CTCAAAATTTAGTAGACTGC+AGG 0.495678 7.4:+92304562 None:intergenic
ATTACCTTATAAGGAAGGGA+TGG 0.507588 7.4:-92304955 MS.gene54465:CDS
AACTTCTTCTTGCCAATAGG+AGG 0.515017 7.4:+92305034 None:intergenic
AGTTGATTACCTTATAAGGA+AGG 0.522099 7.4:-92304960 MS.gene54465:CDS
ATTACTTCCATTACAAGCAA+TGG 0.539617 7.4:-92305242 MS.gene54465:CDS
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.4:+92304755 None:intergenic
CGTGCCTCTTCGGTACAATC+CGG 0.544084 7.4:-92305332 MS.gene54465:CDS
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.4:-92304763 MS.gene54465:CDS
GTTGGACTAAAGTCCATGGC+TGG 0.555609 7.4:-92305293 MS.gene54465:CDS
AACGAATGGAGCCACCGCAC+CGG 0.556419 7.4:+92305313 None:intergenic
TTACCTTATAAGGAAGGGAT+GGG 0.559428 7.4:-92304954 MS.gene54465:CDS
AGCTGAAGAGGACATGATAG+AGG 0.561007 7.4:+92305370 None:intergenic
TTCGGTACAATCCGGTGCGG+TGG 0.565195 7.4:-92305324 MS.gene54465:CDS
GTTGATTACCTTATAAGGAA+GGG 0.567383 7.4:-92304959 MS.gene54465:CDS
TAACGTCCATCATAGTATCC+TGG 0.569613 7.4:+92304745 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.4:-92304751 MS.gene54465:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.4:+92304623 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.4:+92304764 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.597051 7.4:+92305358 None:intergenic
GCATCAGTTGCTCCAAACAA+AGG 0.598394 7.4:+92304703 None:intergenic
ATTCGTTGGACTAAAGTCCA+TGG 0.609669 7.4:-92305297 MS.gene54465:CDS
ACTTCCATTACAAGCAATGG+TGG 0.610706 7.4:-92305239 MS.gene54465:CDS
TTGCTTGGAATTTGAGACCG+AGG 0.613015 7.4:-92304927 MS.gene54465:intron
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.4:+92305280 None:intergenic
GTTCTCTGGTCAATGGTGGA+AGG 0.618328 7.4:+92304996 None:intergenic
ATGGACTTTAGTCCAACGAA+TGG 0.623442 7.4:+92305299 None:intergenic
AATTGTTCTCTGGTCAATGG+TGG 0.637841 7.4:+92304992 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.4:-92305219 MS.gene54465:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.4:+92304638 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.664722 7.4:+92305333 None:intergenic
CGCACCGGATTGTACCGAAG+AGG 0.667423 7.4:+92305328 None:intergenic
CTCTTCGGTACAATCCGGTG+CGG 0.678435 7.4:-92305327 MS.gene54465:CDS
GAATCCGATGATACGGACAA+AGG 0.687909 7.4:+92304630 None:intergenic
ATGGACGTTACTGGACAATG+TGG 0.742197 7.4:-92304732 MS.gene54465:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTAAAGTAATATTAGACCT+CGG + chr7.4:92305040-92305059 None:intergenic 25.0%
! ATAAGCACTTATAGATTCAT+TGG - chr7.4:92304843-92304862 MS.gene54465:intron 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.4:92304983-92305002 MS.gene54465:CDS 25.0%
!! TATATTTTCATAAGCTCTTC+AGG - chr7.4:92304792-92304811 MS.gene54465:intron 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.4:92305136-92305155 MS.gene54465:intron 25.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.4:92304987-92305006 MS.gene54465:CDS 30.0%
ATTACTTCCATTACAAGCAA+TGG - chr7.4:92304705-92304724 MS.gene54465:CDS 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.4:92304683-92304702 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.4:92304988-92305007 MS.gene54465:CDS 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.4:92304684-92304703 None:intergenic 30.0%
!!! ACAAGTTTGTTAACCCTTTT+GGG - chr7.4:92304875-92304894 MS.gene54465:intron 30.0%
ACACCTAAAATTGCCCAAAA+GGG + chr7.4:92304891-92304910 None:intergenic 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.4:92304992-92305011 MS.gene54465:CDS 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.4:92304919-92304938 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.4:92304993-92305012 MS.gene54465:CDS 35.0%
! TAACCCTTTTGGGCAATTTT+AGG - chr7.4:92304885-92304904 MS.gene54465:intron 35.0%
! TTCTTTTGCCAATTGTTCTC+TGG + chr7.4:92304968-92304987 None:intergenic 35.0%
!!! CACAAGTTTGTTAACCCTTT+TGG - chr7.4:92304874-92304893 MS.gene54465:intron 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.4:92304916-92304935 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.4:92305196-92305215 MS.gene54465:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.4:92304958-92304977 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.4:92304957-92304976 MS.gene54465:CDS 40.0%
ACTTCCATTACAAGCAATGG+TGG - chr7.4:92304708-92304727 MS.gene54465:CDS 40.0%
ATGGACTTTAGTCCAACGAA+TGG + chr7.4:92304651-92304670 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.4:92305232-92305251 MS.gene54465:CDS 40.0%
ATTCGTTGGACTAAAGTCCA+TGG - chr7.4:92304650-92304669 MS.gene54465:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.4:92305105-92305124 None:intergenic 40.0%
CACACCTAAAATTGCCCAAA+AGG + chr7.4:92304892-92304911 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.4:92305151-92305170 MS.gene54465:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.4:92305205-92305224 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.4:92304715-92304734 None:intergenic 40.0%
!! CTTTTGGGCAATTTTAGGTG+TGG - chr7.4:92304890-92304909 MS.gene54465:intron 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.4:92305206-92305225 MS.gene54465:intron 40.0%
!! TGATGCTTCTCAAGTGTTGA+AGG - chr7.4:92305261-92305280 MS.gene54465:CDS 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.4:92304592-92304611 None:intergenic 45.0%
AGCTGAAGAGGACATGATAG+AGG + chr7.4:92304580-92304599 None:intergenic 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.4:92305215-92305234 MS.gene54465:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.4:92304999-92305018 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.4:92305195-92305214 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.4:92305327-92305346 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.4:92305320-92305339 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.4:92305313-92305332 MS.gene54465:CDS 45.0%
GCATCAGTTGCTCCAAACAA+AGG + chr7.4:92305247-92305266 None:intergenic 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.4:92304961-92304980 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.4:92304605-92304624 MS.gene54465:CDS 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.4:92304728-92304747 MS.gene54465:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.4:92305020-92305039 MS.gene54465:CDS 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.4:92305313-92305332 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.4:92305312-92305331 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.4:92304901-92304920 MS.gene54465:intron 45.0%
GTTGGACTAAAGTCCATGGC+TGG - chr7.4:92304654-92304673 MS.gene54465:CDS 50.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.4:92305186-92305205 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.4:92304954-92304973 None:intergenic 50.0%
!! TCGTTGTTTTGTCGCGACCA+AGG - chr7.4:92305085-92305104 MS.gene54465:intron 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.4:92304617-92304636 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.4:92305184-92305203 MS.gene54465:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.4:92304615-92304634 MS.gene54465:CDS 55.0%
CTCTTCGGTACAATCCGGTG+CGG - chr7.4:92304620-92304639 MS.gene54465:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.4:92304670-92304689 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.4:92305005-92305024 MS.gene54465:CDS 55.0%
AACGAATGGAGCCACCGCAC+CGG + chr7.4:92304637-92304656 None:intergenic 60.0%
CGCACCGGATTGTACCGAAG+AGG + chr7.4:92304622-92304641 None:intergenic 60.0%
TTCGGTACAATCCGGTGCGG+TGG - chr7.4:92304623-92304642 MS.gene54465:CDS 60.0%
GGTGCGGTGGCTCCATTCGT+TGG - chr7.4:92304636-92304655 MS.gene54465:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 92304570 92305399 92304570 ID=MS.gene54465
chr7.4 mRNA 92304570 92305399 92304570 ID=MS.gene54465.t1;Parent=MS.gene54465
chr7.4 exon 92305220 92305399 92305220 ID=MS.gene54465.t1.exon1;Parent=MS.gene54465.t1
chr7.4 CDS 92305220 92305399 92305220 ID=cds.MS.gene54465.t1;Parent=MS.gene54465.t1
chr7.4 exon 92304928 92305062 92304928 ID=MS.gene54465.t1.exon2;Parent=MS.gene54465.t1
chr7.4 CDS 92304928 92305062 92304928 ID=cds.MS.gene54465.t1;Parent=MS.gene54465.t1
chr7.4 exon 92304570 92304800 92304570 ID=MS.gene54465.t1.exon3;Parent=MS.gene54465.t1
chr7.4 CDS 92304570 92304800 92304570 ID=cds.MS.gene54465.t1;Parent=MS.gene54465.t1
Gene Sequence

>MS.gene54465

ATGGCTTCCTCTATCATGTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGTGCGGTGGCTCCATTCGTTGGACTAAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACTTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAAAATTTATGACATGTCTGTAAACTGTTTTCATCTTATATTTTCATAAGCTCTTCAGGATAGTGTATGAAAACAGCTTATACGTACATAAGCACTTATAGATTCATTGGCAAAAGTACACAAGTTTGTTAACCCTTTTGGGCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCAAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTTACTTGCAATATCTTCTCAATATATATAATCGTTGTTTTGTCGCGACCAAGGTCACAGTCACAAATTTGTACTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACTGATGCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTCTACTAA

Protein sequence

>MS.gene54465.t1

MASSIMSSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQVWPPIGKKKFETLSYLPPLTREQLAKEVDYLIRKGWVACLEFETEKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDASQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY