AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074249

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074249.t1 MTR_6g018300 93.220 177 12 0 1 177 1 177 8.93e-126 351
MS.gene074249.t1 MTR_6g018310 91.111 180 13 1 1 177 1 180 3.93e-123 344
MS.gene074249.t1 MTR_7g007120 83.140 172 25 2 9 177 11 181 8.16e-106 301
MS.gene074249.t1 MTR_7g007120 82.558 172 26 2 9 177 11 181 4.32e-105 299
MS.gene074249.t1 MTR_7g007200 81.977 172 27 2 9 177 11 181 5.09e-105 298
MS.gene074249.t1 MTR_7g007220 81.977 172 27 2 9 177 11 181 8.91e-105 298
MS.gene074249.t1 MTR_7g007120 82.456 171 26 2 10 177 12 181 1.29e-104 298
MS.gene074249.t1 MTR_7g007230 80.702 171 29 2 10 177 12 181 1.63e-102 292
MS.gene074249.t1 MTR_7g007210 81.290 155 25 2 9 160 11 164 4.73e-91 264
MS.gene074249.t1 MTR_4g127870 50.000 156 75 2 23 176 15 169 2.68e-59 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074249.t1 AT1G67090 75.000 172 43 0 6 177 7 178 5.21e-100 286
MS.gene074249.t1 AT5G38430 73.864 176 45 1 2 177 4 178 2.56e-99 284
MS.gene074249.t1 AT5G38430 73.864 176 45 1 2 177 4 178 2.56e-99 284
MS.gene074249.t1 AT5G38410 72.727 176 47 1 2 177 4 178 4.33e-98 281
MS.gene074249.t1 AT5G38420 72.727 176 47 1 2 177 4 178 7.99e-98 280
MS.gene074249.t1 AT5G38410 70.718 181 47 2 2 177 4 183 1.48e-95 275
MS.gene074249.t1 AT5G38410 69.886 176 45 2 2 177 4 171 3.07e-91 263
MS.gene074249.t1 AT1G67090 66.667 105 35 0 6 110 7 111 6.43e-48 152

Find 59 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCAAACTTAGTAGGTTTC+TGG 0.166011 6.2:-14384644 None:intergenic
AATGTCATTGTTGGCCTTCT+TGG 0.287881 6.2:-14383518 None:intergenic
GAAGGGATGGATTCCTTGTT+TGG 0.310858 6.2:+14383939 MS.gene074249:CDS
TTGTTGGCCTTCTTGGTGAC+TGG 0.339143 6.2:-14383511 None:intergenic
AACTCATTCATCAGAATCAT+TGG 0.341056 6.2:+14384572 MS.gene074249:CDS
GATACTATGACGGACGTTAC+TGG 0.345500 6.2:+14384465 MS.gene074249:CDS
ATGATTCTGATGAATGAGTT+TGG 0.359780 6.2:-14384569 None:intergenic
TCAAACTTCTTCTTGCCAAC+TGG 0.385159 6.2:-14383850 None:intergenic
TGATTCTTCTCAAGTGTTGA+AGG 0.390177 6.2:+14384520 MS.gene074249:CDS
ATGTGGAGGTTGCCTTTGTT+CGG 0.417201 6.2:+14384491 MS.gene074249:CDS
ACCCCTCACTGAGGAACAAT+TGG 0.420148 6.2:+14383891 MS.gene074249:CDS
GTGCAAATATCAAACTTAGT+AGG 0.441976 6.2:-14384651 None:intergenic
AGGAAGTAGAATACCTCATC+AGG 0.442741 6.2:+14383917 MS.gene074249:CDS
AGCCAATTGTTCCTCAGTGA+GGG 0.454137 6.2:-14383893 None:intergenic
TAACCGCGTCTCTGCTAACT+TGG 0.454346 6.2:+14383450 MS.gene074249:CDS
TGTTGGCCTTCTTGGTGACT+GGG 0.456367 6.2:-14383510 None:intergenic
GTTAGCAGAGACGCGGTTAA+CGG 0.461063 6.2:-14383446 None:intergenic
ACCGGTCTCAAATCCTCAGC+TGG 0.472739 6.2:+14383484 MS.gene074249:CDS
TGATTCTGATGAATGAGTTT+GGG 0.474330 6.2:-14384568 None:intergenic
AACTTGGTTGCTCCATTCAC+CGG 0.479187 6.2:+14383466 MS.gene074249:CDS
GAGGATTTGAGACCGGTGAA+TGG 0.484712 6.2:-14383478 None:intergenic
CGTGAGAACCACCGTTCACC+AGG 0.497088 6.2:+14384443 MS.gene074249:CDS
TCCACCATTGCTTGCAATGG+AGG 0.500934 6.2:-14383542 None:intergenic
AACGGTGGTAACAGCGGCGG+AGG 0.513043 6.2:-14383428 None:intergenic
TCTTCCACCATTGCTTGCAA+TGG 0.515588 6.2:-14383545 None:intergenic
TTGTTTGGAATTTGAGCTTG+AGG 0.518744 6.2:+14383954 MS.gene074249:CDS
AACTTCTTCTTGCCAACTGG+TGG 0.532490 6.2:-14383847 None:intergenic
CACTGAGGAACAATTGGCTA+AGG 0.537360 6.2:+14383897 MS.gene074249:CDS
AATGGAGGTAATGTCATTGT+TGG 0.544075 6.2:-14383527 None:intergenic
TAGCCAATTGTTCCTCAGTG+AGG 0.553733 6.2:-14383894 None:intergenic
TAACGTCCGTCATAGTATCC+TGG 0.560465 6.2:-14384461 None:intergenic
AATACCTCATCAGGAAGGGA+TGG 0.561983 6.2:+14383926 MS.gene074249:CDS
CTGGTGAACGGTGGTTCTCA+CGG 0.562589 6.2:-14384442 None:intergenic
ACCTCCATTGCAAGCAATGG+TGG 0.570031 6.2:+14383541 MS.gene074249:CDS
GTTCCTCAGTGAGGGGTGGA+AGG 0.571475 6.2:-14383885 None:intergenic
ACCAGCTGAGGATTTGAGAC+CGG 0.573385 6.2:-14383485 None:intergenic
GAATCCATCCCTTCCTGATG+AGG 0.575992 6.2:-14383930 None:intergenic
GCCAATTGTTCCTCAGTGAG+GGG 0.576725 6.2:-14383892 None:intergenic
AGTAGAATACCTCATCAGGA+AGG 0.582514 6.2:+14383921 MS.gene074249:CDS
GAATCAGTGGCTCCGAACAA+AGG 0.592010 6.2:-14384503 None:intergenic
TTATAGGTGTGGCCACCAGT+TGG 0.602882 6.2:+14383835 MS.gene074249:intron
TGGTTTCCCAGTCACCAAGA+AGG 0.603110 6.2:+14383504 MS.gene074249:CDS
ATTACCTCCATTGCAAGCAA+TGG 0.615000 6.2:+14383538 MS.gene074249:CDS
AGCAGAGACGCGGTTAACGG+TGG 0.615227 6.2:-14383443 None:intergenic
TCAAGCTCAAATTCCAAACA+AGG 0.620566 6.2:-14383952 None:intergenic
TGGAAGAGTGAACTGTATGC+AGG 0.631476 6.2:+14383561 MS.gene074249:CDS
CAACACTTGAGAAGAATCAG+TGG 0.638041 6.2:-14384516 None:intergenic
CGTCATAGTATCCTGGTGAA+CGG 0.638410 6.2:-14384454 None:intergenic
AATTGTTCCTCAGTGAGGGG+TGG 0.640814 6.2:-14383889 None:intergenic
GTAGAATACCTCATCAGGAA+GGG 0.642395 6.2:+14383922 MS.gene074249:CDS
GGTGACTGGGAAACCAGCTG+AGG 0.655087 6.2:-14383497 None:intergenic
CATAGTATCCTGGTGAACGG+TGG 0.657211 6.2:-14384451 None:intergenic
GCGGTTAACGGTGGTAACAG+CGG 0.666203 6.2:-14383434 None:intergenic
GACGTTACTGGACAATGTGG+AGG 0.672886 6.2:+14384477 MS.gene074249:CDS
CAACCAAGTTAGCAGAGACG+CGG 0.682316 6.2:-14383453 None:intergenic
GTTAACGGTGGTAACAGCGG+CGG 0.699396 6.2:-14383431 None:intergenic
CGTTCACCAGGATACTATGA+CGG 0.703631 6.2:+14384455 MS.gene074249:CDS
ACGGACGTTACTGGACAATG+TGG 0.708236 6.2:+14384474 MS.gene074249:CDS
ATACCTTCCACCCCTCACTG+AGG 0.719780 6.2:+14383882 MS.gene074249:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATATATACAATAAAG+GGG - chr6.2:14384095-14384114 None:intergenic 10.0%
!!! ACATGAAATTAAATTTTATA+CGG - chr6.2:14383624-14383643 None:intergenic 10.0%
!!! ATATGTAAAATATTTTTACA+CGG - chr6.2:14384166-14384185 None:intergenic 10.0%
!! AAACACTATAAAAATATCAT+AGG - chr6.2:14384204-14384223 None:intergenic 15.0%
!! ATACATAAAATTGAGTATTA+GGG - chr6.2:14383810-14383829 None:intergenic 15.0%
!! ATGTCACAAAAAAATAAAAT+TGG + chr6.2:14383706-14383725 MS.gene074249:intron 15.0%
!! TATACATAAAATTGAGTATT+AGG - chr6.2:14383811-14383830 None:intergenic 15.0%
!! ATGTATAATATTTATAGGTG+TGG + chr6.2:14383824-14383843 MS.gene074249:intron 20.0%
!! ATTAAACTCGTAAGTTAAAA+AGG - chr6.2:14384010-14384029 None:intergenic 20.0%
!! GTAAGTTAAAAAGGAAAAAA+AGG - chr6.2:14384001-14384020 None:intergenic 20.0%
!! TCATAATTTAAGTAACCTTA+AGG - chr6.2:14383666-14383685 None:intergenic 20.0%
!! TTAATTAATTGTGTCAACTT+TGG - chr6.2:14383768-14383787 None:intergenic 20.0%
!!! ATTTGTTGTGTTATATAGAA+AGG + chr6.2:14384410-14384429 MS.gene074249:intron 20.0%
!!! GATATTTTTATAGTGTTTAG+TGG + chr6.2:14384206-14384225 MS.gene074249:intron 20.0%
!!! GTAAAAAAGTTTTTCATTGT+CGG - chr6.2:14384042-14384061 None:intergenic 20.0%
! ATACGGTATATGCAAAAATA+TGG - chr6.2:14383607-14383626 None:intergenic 25.0%
! TATATGCAAAAATATGGACT+AGG - chr6.2:14383601-14383620 None:intergenic 25.0%
! TTACATGTTTATGTACCTTA+AGG + chr6.2:14383648-14383667 MS.gene074249:intron 25.0%
! TTATAGTGTTTAGTGGAATT+TGG + chr6.2:14384213-14384232 MS.gene074249:intron 25.0%
!! ATATGCAAAAATATGGACTA+GGG - chr6.2:14383600-14383619 None:intergenic 25.0%
AACTCATTCATCAGAATCAT+TGG + chr6.2:14384572-14384591 MS.gene074249:CDS 30.0%
ATGATTCTGATGAATGAGTT+TGG - chr6.2:14384572-14384591 None:intergenic 30.0%
TGATTCTGATGAATGAGTTT+GGG - chr6.2:14384571-14384590 None:intergenic 30.0%
AATGGAGGTAATGTCATTGT+TGG - chr6.2:14383530-14383549 None:intergenic 35.0%
CAGGTTGTTGGAATTTGATA+TGG + chr6.2:14384279-14384298 MS.gene074249:intron 35.0%
GCGTCATGATAAAATCTGAT+TGG - chr6.2:14384073-14384092 None:intergenic 35.0%
TCAAGCTCAAATTCCAAACA+AGG - chr6.2:14383955-14383974 None:intergenic 35.0%
! TTGTTTGGAATTTGAGCTTG+AGG + chr6.2:14383954-14383973 MS.gene074249:CDS 35.0%
!! AAAAATATGGACTAGGGCTT+GGG - chr6.2:14383594-14383613 None:intergenic 35.0%
!! TGATTCTTCTCAAGTGTTGA+AGG + chr6.2:14384520-14384539 MS.gene074249:CDS 35.0%
AATCCTCTCATTCACTCAGT+CGG + chr6.2:14384252-14384271 MS.gene074249:intron 40.0%
ACCCTACGAAATTCATCACT+CGG - chr6.2:14384380-14384399 None:intergenic 40.0%
AGGAAGTAGAATACCTCATC+AGG + chr6.2:14383917-14383936 MS.gene074249:CDS 40.0%
AGTAGAATACCTCATCAGGA+AGG + chr6.2:14383921-14383940 MS.gene074249:CDS 40.0%
ATTACCTCCATTGCAAGCAA+TGG + chr6.2:14383538-14383557 MS.gene074249:CDS 40.0%
CAACACTTGAGAAGAATCAG+TGG - chr6.2:14384519-14384538 None:intergenic 40.0%
GTAGAATACCTCATCAGGAA+GGG + chr6.2:14383922-14383941 MS.gene074249:CDS 40.0%
TCAAACTTCTTCTTGCCAAC+TGG - chr6.2:14383853-14383872 None:intergenic 40.0%
TCAGCACATCATGTAGTTGT+TGG - chr6.2:14384307-14384326 None:intergenic 40.0%
TCCGAGTGATGAATTTCGTA+GGG + chr6.2:14384376-14384395 MS.gene074249:intron 40.0%
TTCCGAGTGATGAATTTCGT+AGG + chr6.2:14384375-14384394 MS.gene074249:intron 40.0%
! ATTCGGATTGTCTGAAGTCA+AGG - chr6.2:14384350-14384369 None:intergenic 40.0%
!! AATGTCATTGTTGGCCTTCT+TGG - chr6.2:14383521-14383540 None:intergenic 40.0%
!! CAAAAATATGGACTAGGGCT+TGG - chr6.2:14383595-14383614 None:intergenic 40.0%
AACTTCTTCTTGCCAACTGG+TGG - chr6.2:14383850-14383869 None:intergenic 45.0%
AACTTGGTTGCTCCATTCAC+CGG + chr6.2:14383466-14383485 MS.gene074249:CDS 45.0%
AATACCTCATCAGGAAGGGA+TGG + chr6.2:14383926-14383945 MS.gene074249:CDS 45.0%
AGCCAATTGTTCCTCAGTGA+GGG - chr6.2:14383896-14383915 None:intergenic 45.0%
ATGTGGAGGTTGCCTTTGTT+CGG + chr6.2:14384491-14384510 MS.gene074249:CDS 45.0%
CACTGAGGAACAATTGGCTA+AGG + chr6.2:14383897-14383916 MS.gene074249:CDS 45.0%
CATCACTCGGAACACGAATT+CGG - chr6.2:14384367-14384386 None:intergenic 45.0%
CGTCATAGTATCCTGGTGAA+CGG - chr6.2:14384457-14384476 None:intergenic 45.0%
CGTTCACCAGGATACTATGA+CGG + chr6.2:14384455-14384474 MS.gene074249:CDS 45.0%
TAACGTCCGTCATAGTATCC+TGG - chr6.2:14384464-14384483 None:intergenic 45.0%
TAGCCAATTGTTCCTCAGTG+AGG - chr6.2:14383897-14383916 None:intergenic 45.0%
TCTTCCACCATTGCTTGCAA+TGG - chr6.2:14383548-14383567 None:intergenic 45.0%
TGGAAGAGTGAACTGTATGC+AGG + chr6.2:14383561-14383580 MS.gene074249:CDS 45.0%
! ATGAGAGGATTATCGACTGC+AGG - chr6.2:14384243-14384262 None:intergenic 45.0%
! GAAGGGATGGATTCCTTGTT+TGG + chr6.2:14383939-14383958 MS.gene074249:CDS 45.0%
!! ATGTGCTGACTCTGTGAATG+CGG + chr6.2:14384318-14384337 MS.gene074249:intron 45.0%
!! GATACTATGACGGACGTTAC+TGG + chr6.2:14384465-14384484 MS.gene074249:CDS 45.0%
!! AAAATAAATATATACAATAA+AGG - chr6.2:14384097-14384116 None:intergenic 5.0%
!! AAATAAATATATACAATAAA+GGG - chr6.2:14384096-14384115 None:intergenic 5.0%
!!! ATTTTATGTATAATATTTAT+AGG + chr6.2:14383819-14383838 MS.gene074249:intron 5.0%
AATTGTTCCTCAGTGAGGGG+TGG - chr6.2:14383892-14383911 None:intergenic 50.0%
ACCCCTCACTGAGGAACAAT+TGG + chr6.2:14383891-14383910 MS.gene074249:CDS 50.0%
ACCTCCATTGCAAGCAATGG+TGG + chr6.2:14383541-14383560 MS.gene074249:CDS 50.0%
ACGGACGTTACTGGACAATG+TGG + chr6.2:14384474-14384493 MS.gene074249:CDS 50.0%
CAACCAAGTTAGCAGAGACG+CGG - chr6.2:14383456-14383475 None:intergenic 50.0%
CATAGTATCCTGGTGAACGG+TGG - chr6.2:14384454-14384473 None:intergenic 50.0%
CATTCACTCAGTCGGTACAC+AGG + chr6.2:14384260-14384279 MS.gene074249:intron 50.0%
GAATCAGTGGCTCCGAACAA+AGG - chr6.2:14384506-14384525 None:intergenic 50.0%
GAATCCATCCCTTCCTGATG+AGG - chr6.2:14383933-14383952 None:intergenic 50.0%
GACGTTACTGGACAATGTGG+AGG + chr6.2:14384477-14384496 MS.gene074249:CDS 50.0%
GCCAATTGTTCCTCAGTGAG+GGG - chr6.2:14383895-14383914 None:intergenic 50.0%
GTTAGCAGAGACGCGGTTAA+CGG - chr6.2:14383449-14383468 None:intergenic 50.0%
TAACCGCGTCTCTGCTAACT+TGG + chr6.2:14383450-14383469 MS.gene074249:CDS 50.0%
TCAGTCGGTACACAGGTTGT+TGG + chr6.2:14384267-14384286 MS.gene074249:intron 50.0%
TCCACCATTGCTTGCAATGG+AGG - chr6.2:14383545-14383564 None:intergenic 50.0%
TGGTTTCCCAGTCACCAAGA+AGG + chr6.2:14383504-14383523 MS.gene074249:CDS 50.0%
TTATAGGTGTGGCCACCAGT+TGG + chr6.2:14383835-14383854 MS.gene074249:intron 50.0%
! ACCAGCTGAGGATTTGAGAC+CGG - chr6.2:14383488-14383507 None:intergenic 50.0%
! GAGGATTTGAGACCGGTGAA+TGG - chr6.2:14383481-14383500 None:intergenic 50.0%
!! GTACCGACTGAGTGAATGAG+AGG - chr6.2:14384258-14384277 None:intergenic 50.0%
!! TGTTGGCCTTCTTGGTGACT+GGG - chr6.2:14383513-14383532 None:intergenic 50.0%
!! TTGTTGGCCTTCTTGGTGAC+TGG - chr6.2:14383514-14383533 None:intergenic 50.0%
ACCGGTCTCAAATCCTCAGC+TGG + chr6.2:14383484-14383503 MS.gene074249:CDS 55.0%
ATACCTTCCACCCCTCACTG+AGG + chr6.2:14383882-14383901 MS.gene074249:CDS 55.0%
CTGGTGAACGGTGGTTCTCA+CGG - chr6.2:14384445-14384464 None:intergenic 55.0%
GCGGTTAACGGTGGTAACAG+CGG - chr6.2:14383437-14383456 None:intergenic 55.0%
GTTAACGGTGGTAACAGCGG+CGG - chr6.2:14383434-14383453 None:intergenic 55.0%
AGCAGAGACGCGGTTAACGG+TGG - chr6.2:14383446-14383465 None:intergenic 60.0%
CGTGAGAACCACCGTTCACC+AGG + chr6.2:14384443-14384462 MS.gene074249:CDS 60.0%
GGTGACTGGGAAACCAGCTG+AGG - chr6.2:14383500-14383519 None:intergenic 60.0%
GTTCCTCAGTGAGGGGTGGA+AGG - chr6.2:14383888-14383907 None:intergenic 60.0%
AACGGTGGTAACAGCGGCGG+AGG - chr6.2:14383431-14383450 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 14383415 14384658 14383415 ID=MS.gene074249
chr6.2 mRNA 14383415 14384658 14383415 ID=MS.gene074249.t1;Parent=MS.gene074249
chr6.2 exon 14383415 14383582 14383415 ID=MS.gene074249.t1.exon1;Parent=MS.gene074249.t1
chr6.2 CDS 14383415 14383582 14383415 ID=cds.MS.gene074249.t1;Parent=MS.gene074249.t1
chr6.2 exon 14383841 14383975 14383841 ID=MS.gene074249.t1.exon2;Parent=MS.gene074249.t1
chr6.2 CDS 14383841 14383975 14383841 ID=cds.MS.gene074249.t1;Parent=MS.gene074249.t1
chr6.2 exon 14384428 14384658 14384428 ID=MS.gene074249.t1.exon3;Parent=MS.gene074249.t1
chr6.2 CDS 14384428 14384658 14384428 ID=cds.MS.gene074249.t1;Parent=MS.gene074249.t1
Gene Sequence

>MS.gene074249

ATGGCTTTGATCTCCTCCGCCGCTGTTACCACCGTTAACCGCGTCTCTGCTAACTTGGTTGCTCCATTCACCGGTCTCAAATCCTCAGCTGGTTTCCCAGTCACCAAGAAGGCCAACAATGACATTACCTCCATTGCAAGCAATGGTGGAAGAGTGAACTGTATGCAGGTAATACACCCAAGCCCTAGTCCATATTTTTGCATATACCGTATAAAATTTAATTTCATGTTATGTTACATGTTTATGTACCTTAAGGTTACTTAAATTATGAATCATGTCATAAAAAATAGAATGTCACAAAAAAATAAAATTGGACTATAATTCTAGATATATTTTATCTACAAAGTATACCAAAGTTGACACAATTAATTAAAATTGTCAAATTTTGTTAACCCTAATACTCAATTTTATGTATAATATTTATAGGTGTGGCCACCAGTTGGCAAGAAGAAGTTTGAGACACTTTCATACCTTCCACCCCTCACTGAGGAACAATTGGCTAAGGAAGTAGAATACCTCATCAGGAAGGGATGGATTCCTTGTTTGGAATTTGAGCTTGAGGTTAATCTATTTACTATTGCAACCTTTTTTTCCTTTTTAACTTACGAGTTTAATTACTATACACCGACAATGAAAAACTTTTTTACATTTACAACCAATCAGATTTTATCATGACGCCCCTTTATTGTATATATTTATTTTTAAAATATCTCAACAAGCATTTACGTTGCAAAATCTGATTGAATGACCGTGTAAAAATATTTTACATATATACTCAATATATAGCCTATGATATTTTTATAGTGTTTAGTGGAATTTGGATTACCTGCAGTCGATAATCCTCTCATTCACTCAGTCGGTACACAGGTTGTTGGAATTTGATATGGATCCAACAACTACATGATGTGCTGACTCTGTGAATGCGGATAATTCCTTGACTTCAGACAATCCGAATTCGTGTTCCGAGTGATGAATTTCGTAGGGTTGATGTGTTAATTTGTTGTGTTATATAGAAAGGATTTGTCTACCGTGAGAACCACCGTTCACCAGGATACTATGACGGACGTTACTGGACAATGTGGAGGTTGCCTTTGTTCGGAGCCACTGATTCTTCTCAAGTGTTGAAGGAGCTTGCTGACTGTAAAGCTGAATACCCAAACTCATTCATCAGAATCATTGGATTTGACAATGTTCGTCAAGTGCAGTGCATTAGTTTCATTGCACACACACCAGAAACCTACTAA
Protein sequence

>MS.gene074249.t1

MALISSAAVTTVNRVSANLVAPFTGLKSSAGFPVTKKANNDITSIASNGGRVNCMQVWPPVGKKKFETLSYLPPLTEEQLAKEVEYLIRKGWIPCLEFELEKGFVYRENHRSPGYYDGRYWTMWRLPLFGATDSSQVLKELADCKAEYPNSFIRIIGFDNVRQVQCISFIAHTPETY