AlfalfaGEDB | MS.gene96213

M. sativa cultivar XinJiangDaYe / MS.gene96213

KEGG: K03676
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00462.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96213.t1	XP_003593970.1	99	102	1	0	1	102	1	102	1.20E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96213.t1	Q8L8Z8	76.5	102	24	0	1	102	1	102	1.3e-40	166.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96213.t1	G7IQ03	99.0	102	1	0	1	102	1	102	8.8e-51	208.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene96213	0.804226	2.39E-49	-1.69E-46
MS.gene049285	MS.gene96213	0.80421	2.41E-49	-1.69E-46
MS.gene049320	MS.gene96213	0.816365	5.74E-52	-1.69E-46
MS.gene049369	MS.gene96213	0.821171	4.65E-53	-1.69E-46
MS.gene049427	MS.gene96213	0.805068	1.60E-49	-1.69E-46
MS.gene049805	MS.gene96213	0.807788	4.26E-50	-1.69E-46
MS.gene050333	MS.gene96213	0.814168	1.77E-51	-1.69E-46
MS.gene050432	MS.gene96213	0.83753	4.92E-57	-1.69E-46
MS.gene050444	MS.gene96213	0.821148	4.71E-53	-1.69E-46
MS.gene050612	MS.gene96213	0.808407	3.15E-50	-1.69E-46
MS.gene050767	MS.gene96213	0.82205	2.91E-53	-1.69E-46
MS.gene050770	MS.gene96213	0.824828	6.53E-54	-1.69E-46
MS.gene050772	MS.gene96213	0.831128	1.99E-55	-1.69E-46
MS.gene05080	MS.gene96213	0.801129	1.04E-48	-1.69E-46
MS.gene051228	MS.gene96213	0.904821	8.22E-80	-1.69E-46
MS.gene051617	MS.gene96213	0.812354	4.42E-51	-1.69E-46
MS.gene051666	MS.gene96213	0.805197	1.50E-49	-1.69E-46
MS.gene051810	MS.gene96213	0.819369	1.20E-52	-1.69E-46
MS.gene052271	MS.gene96213	0.802227	6.20E-49	-1.69E-46
MS.gene052290	MS.gene96213	0.840653	7.64E-58	-1.69E-46
MS.gene052379	MS.gene96213	0.832944	7.08E-56	-1.69E-46
MS.gene052484	MS.gene96213	0.800194	1.62E-48	-1.69E-46
MS.gene052870	MS.gene96213	0.811132	8.16E-51	-1.69E-46
MS.gene052872	MS.gene96213	0.808537	2.95E-50	-1.69E-46
MS.gene053009	MS.gene96213	0.820092	8.23E-53	-1.69E-46
MS.gene053103	MS.gene96213	0.847393	1.19E-59	-1.69E-46
MS.gene053104	MS.gene96213	0.865667	5.00E-65	-1.69E-46
MS.gene053207	MS.gene96213	0.810253	1.26E-50	-1.69E-46
MS.gene053213	MS.gene96213	0.800617	1.32E-48	-1.69E-46
MS.gene053322	MS.gene96213	0.816993	4.15E-52	-1.69E-46
MS.gene053384	MS.gene96213	0.82828	9.81E-55	-1.69E-46
MS.gene053449	MS.gene96213	0.826392	2.78E-54	-1.69E-46
MS.gene053632	MS.gene96213	0.81626	6.06E-52	-1.69E-46
MS.gene053916	MS.gene96213	0.845695	3.46E-59	-1.69E-46
MS.gene054376	MS.gene96213	0.812913	3.34E-51	-1.69E-46
MS.gene054600	MS.gene96213	0.806432	8.25E-50	-1.69E-46
MS.gene054642	MS.gene96213	0.842225	2.95E-58	-1.69E-46
MS.gene05505	MS.gene96213	0.807907	4.02E-50	-1.69E-46
MS.gene055206	MS.gene96213	0.810944	8.96E-51	-1.69E-46
MS.gene055212	MS.gene96213	0.81862	1.78E-52	-1.69E-46
MS.gene055254	MS.gene96213	0.840898	6.59E-58	-1.69E-46
MS.gene055397	MS.gene96213	0.842178	3.03E-58	-1.69E-46
MS.gene055636	MS.gene96213	0.804478	2.12E-49	-1.69E-46
MS.gene055722	MS.gene96213	0.826875	2.13E-54	-1.69E-46
MS.gene05592	MS.gene96213	0.864871	8.90E-65	-1.69E-46
MS.gene055956	MS.gene96213	-0.803831	2.89E-49	-1.69E-46
MS.gene056374	MS.gene96213	0.811156	8.06E-51	-1.69E-46
MS.gene056487	MS.gene96213	0.840926	6.48E-58	-1.69E-46
MS.gene056609	MS.gene96213	0.840939	6.43E-58	-1.69E-46
MS.gene05664	MS.gene96213	0.801608	8.31E-49	-1.69E-46
MS.gene056657	MS.gene96213	0.821892	3.17E-53	-1.69E-46
MS.gene056897	MS.gene96213	0.814744	1.32E-51	-1.69E-46
MS.gene057129	MS.gene96213	0.850853	1.30E-60	-1.69E-46
MS.gene057246	MS.gene96213	0.855842	4.81E-62	-1.69E-46
MS.gene057331	MS.gene96213	0.828501	8.68E-55	-1.69E-46
MS.gene05735	MS.gene96213	0.825556	4.40E-54	-1.69E-46
MS.gene057416	MS.gene96213	0.818758	1.66E-52	-1.69E-46
MS.gene057926	MS.gene96213	0.836368	9.75E-57	-1.69E-46
MS.gene057927	MS.gene96213	0.826938	2.06E-54	-1.69E-46
MS.gene058419	MS.gene96213	0.809636	1.72E-50	-1.69E-46
MS.gene058459	MS.gene96213	0.810898	9.17E-51	-1.69E-46
MS.gene058723	MS.gene96213	0.819967	8.79E-53	-1.69E-46
MS.gene058773	MS.gene96213	0.807909	4.01E-50	-1.69E-46
MS.gene059029	MS.gene96213	0.800914	1.15E-48	-1.69E-46
MS.gene05913	MS.gene96213	0.802207	6.26E-49	-1.69E-46
MS.gene059711	MS.gene96213	0.820245	7.59E-53	-1.69E-46
MS.gene059960	MS.gene96213	0.841978	3.42E-58	-1.69E-46
MS.gene060728	MS.gene96213	0.806534	7.85E-50	-1.69E-46
MS.gene060804	MS.gene96213	0.84299	1.85E-58	-1.69E-46
MS.gene060818	MS.gene96213	0.875897	2.14E-68	-1.69E-46
MS.gene061041	MS.gene96213	0.852915	3.37E-61	-1.69E-46
MS.gene061051	MS.gene96213	-0.817241	3.65E-52	-1.69E-46
MS.gene061134	MS.gene96213	0.805025	1.63E-49	-1.69E-46
MS.gene061200	MS.gene96213	0.822352	2.48E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96213.t1	MTR_2g019900	99.020	102	1	0	1	102	1	102	1.20e-71	208
MS.gene96213.t1	MTR_4g119050	78.641	103	21	1	1	102	1	103	8.25e-56	168
MS.gene96213.t1	MTR_4g119030	78.641	103	21	1	1	102	1	103	8.25e-56	168
MS.gene96213.t1	MTR_4g119040	78.641	103	21	1	1	102	1	103	8.25e-56	168
MS.gene96213.t1	MTR_2g019950	63.725	102	37	0	1	102	1	102	9.74e-45	140
MS.gene96213.t1	MTR_7g108250	52.941	102	48	0	1	102	1	102	5.93e-40	128
MS.gene96213.t1	MTR_1g088905	53.922	102	47	0	1	102	1	102	6.69e-40	128
MS.gene96213.t1	MTR_1g088910	58.416	101	42	0	1	101	1	101	3.91e-39	126
MS.gene96213.t1	MTR_1g088920	58.947	95	39	0	1	95	1	95	6.25e-39	125
MS.gene96213.t1	MTR_1g088925	56.842	95	41	0	1	95	1	95	7.37e-39	125
MS.gene96213.t1	MTR_7g108200	55.789	95	42	0	1	95	1	95	2.20e-37	122
MS.gene96213.t1	MTR_7g022710	53.398	103	47	1	1	102	30	132	1.51e-36	120
MS.gene96213.t1	MTR_7g108220	55.882	102	44	1	1	101	1	102	2.67e-36	119
MS.gene96213.t1	MTR_7g026770	57.843	102	42	1	1	101	22	123	3.58e-36	124
MS.gene96213.t1	MTR_7g022690	52.427	103	48	1	1	102	30	132	1.32e-35	118
MS.gene96213.t1	MTR_7g108210	54.902	102	45	1	1	101	1	102	1.64e-35	117
MS.gene96213.t1	MTR_7g022550	51.456	103	49	1	1	102	30	132	5.52e-35	116
MS.gene96213.t1	MTR_1g088895	50.000	102	51	0	1	102	1	102	4.74e-31	105
MS.gene96213.t1	MTR_7g108260	44.554	101	56	0	1	101	1	101	8.09e-29	100
MS.gene96213.t1	MTR_2g048970	46.667	105	52	1	2	102	29	133	3.86e-25	91.7
MS.gene96213.t1	MTR_2g014760	41.346	104	51	3	4	102	29	127	4.04e-23	86.7
MS.gene96213.t1	MTR_2g090755	37.864	103	57	2	4	102	41	140	1.24e-22	85.5
MS.gene96213.t1	MTR_4g079110	39.000	100	56	2	6	102	58	155	4.89e-21	82.0
MS.gene96213.t1	MTR_1g015890	43.137	102	56	1	3	102	48	149	6.53e-21	81.6
MS.gene96213.t1	MTR_7g035245	40.000	100	60	0	1	100	3	102	2.30e-20	79.0
MS.gene96213.t1	MTR_3g077570	38.000	100	62	0	1	100	18	117	2.90e-19	76.6
MS.gene96213.t1	MTR_3g077560	37.000	100	63	0	1	100	21	120	2.02e-18	74.7
MS.gene96213.t1	MTR_3g104510	39.604	101	53	3	3	101	23	117	1.51e-17	72.0
MS.gene96213.t1	MTR_5g077550	40.777	103	59	1	2	102	21	123	2.50e-16	68.9
MS.gene96213.t1	MTR_1g069255	36.364	99	62	1	2	99	84	182	3.86e-15	67.4
MS.gene96213.t1	MTR_2g038560	32.258	93	63	0	2	94	29	121	1.63e-11	57.0
MS.gene96213.t1	MTR_2g038560	33.333	84	56	0	2	85	70	153	3.01e-11	57.0
MS.gene96213.t1	MTR_2g038560	33.333	84	56	0	2	85	73	156	3.08e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96213.t1	AT5G18600	76.471	102	24	0	1	102	1	102	4.69e-55	166
MS.gene96213.t1	AT4G15690	72.549	102	28	0	1	102	1	102	1.00e-54	166
MS.gene96213.t1	AT4G15680	69.608	102	31	0	1	102	1	102	7.87e-54	163
MS.gene96213.t1	AT4G15670	69.608	102	31	0	1	102	1	102	2.14e-53	162
MS.gene96213.t1	AT4G15700	70.588	102	30	0	1	102	1	102	3.46e-53	161
MS.gene96213.t1	AT4G15660	68.627	102	32	0	1	102	1	102	7.89e-52	158
MS.gene96213.t1	AT1G03020	59.804	102	41	0	1	102	1	102	3.26e-43	136
MS.gene96213.t1	AT3G62930	58.824	102	42	0	1	102	1	102	3.15e-42	134
MS.gene96213.t1	AT3G21460	59.804	102	41	0	1	102	1	102	2.12e-41	132
MS.gene96213.t1	AT2G47870	57.282	103	43	1	1	102	1	103	3.47e-40	129
MS.gene96213.t1	AT1G06830	55.789	95	42	0	1	95	1	95	6.47e-39	125
MS.gene96213.t1	AT2G47880	54.000	100	46	0	1	100	1	100	1.52e-38	124
MS.gene96213.t1	AT3G62950	53.398	103	47	1	1	102	1	103	2.54e-38	124
MS.gene96213.t1	AT2G30540	55.789	95	42	0	1	95	1	95	3.50e-38	124
MS.gene96213.t1	AT3G62960	52.632	95	45	0	1	95	1	95	8.85e-36	117
MS.gene96213.t1	AT5G14070	49.091	110	48	2	1	102	31	140	6.92e-33	111
MS.gene96213.t1	AT3G02000	52.830	106	44	1	3	102	31	136	1.37e-31	108
MS.gene96213.t1	AT4G33040	42.478	113	43	3	3	102	41	144	5.03e-21	81.6
MS.gene96213.t1	AT1G28480	35.238	105	64	1	2	102	33	137	1.52e-20	80.1
MS.gene96213.t1	AT5G63030	39.796	98	59	0	3	100	21	118	1.57e-19	77.4
MS.gene96213.t1	AT5G11930	43.000	100	47	3	3	101	57	147	4.15e-19	77.0
MS.gene96213.t1	AT5G40370	37.000	100	63	0	1	100	3	102	7.78e-19	75.1
MS.gene96213.t1	AT1G03850	37.634	93	54	1	10	102	62	150	4.49e-16	69.3
MS.gene96213.t1	AT4G28730	37.113	97	60	1	2	97	71	167	1.72e-14	65.5
MS.gene96213.t1	AT5G40370	38.372	86	53	0	15	100	42	127	1.20e-13	62.4
MS.gene96213.t1	AT2G20270	35.052	97	62	1	2	97	76	172	2.34e-13	62.8
MS.gene96213.t1	AT5G20500	34.737	95	62	0	4	98	35	129	1.37e-12	59.7

Find 27 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGAAGAGCAGGAGCTATT+TGG	0.243476	2.2:+64955065	MS.gene96213:CDS
TCATCAAGTTCATGAACTTC+TGG	0.278380	2.2:-64954896	None:intergenic
TTGGAATCAAAGACCTATTA+AGG	0.291286	2.2:-64955039	None:intergenic
TTATGAGCCTTCACCTTAAT+AGG	0.313253	2.2:+64955026	MS.gene96213:CDS
TAGAAGAGCAGGAGCTATTT+GGG	0.338414	2.2:+64955066	MS.gene96213:CDS
TCTGTGCCTGCTGTGTTCAT+TGG	0.345825	2.2:+64954974	MS.gene96213:CDS
CACTCCAAAGTCATTGAAAA+GGG	0.415156	2.2:-64954870	None:intergenic
TCACTCCAAAGTCATTGAAA+AGG	0.427326	2.2:-64954871	None:intergenic
TTCATTGGTGGTGAGCTTGT+TGG	0.448261	2.2:+64954989	MS.gene96213:CDS
GTGCCTGCTGTGTTCATTGG+TGG	0.469372	2.2:+64954977	MS.gene96213:CDS
ATTCCAATGCTTAGAAGAGC+AGG	0.473528	2.2:+64955055	MS.gene96213:CDS
GCTCCTGCTCTTCTAAGCAT+TGG	0.494845	2.2:-64955058	None:intergenic
ATTGGTGGTGAGCTTGTTGG+TGG	0.505118	2.2:+64954992	MS.gene96213:CDS
ATGAACACAGCAGGCACAGA+AGG	0.552108	2.2:-64954971	None:intergenic
TCACCACCAATGAACACAGC+AGG	0.561831	2.2:-64954980	None:intergenic
TCAAAGACCTATTAAGGTGA+AGG	0.569141	2.2:-64955033	None:intergenic
CAACCTTACATTAATGGAGA+GGG	0.591764	2.2:+64954772	None:intergenic
AATGGAGAGGGTGACACAGT+TGG	0.593802	2.2:+64954784	None:intergenic
ATGAACTTGATGAAATGAGT+GGG	0.613266	2.2:+64954906	MS.gene96213:CDS
TCAACCTTACATTAATGGAG+AGG	0.626935	2.2:+64954771	None:intergenic
TGAACACAGCAGGCACAGAA+GGG	0.649613	2.2:-64954970	None:intergenic
CATGAACTTGATGAAATGAG+TGG	0.682020	2.2:+64954905	MS.gene96213:CDS
GAACTTGATGAAATGAGTGG+GGG	0.688177	2.2:+64954908	MS.gene96213:CDS
TGAACTTGATGAAATGAGTG+GGG	0.691196	2.2:+64954907	MS.gene96213:CDS
GTTGGCATCAGAGAGGTCAG+TGG	0.695839	2.2:+64954802	MS.gene96213:CDS
AACTTGATGAAATGAGTGGG+GGG	0.697784	2.2:+64954909	MS.gene96213:CDS
TGACACAGTTGGCATCAGAG+AGG	0.724593	2.2:+64954795	MS.gene96213:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGAACTTGATGAAATGAGT+GGG	+	chr2.2:64954906-64954925	MS.gene96213:CDS	30.0%
	TTGGAATCAAAGACCTATTA+AGG	-	chr2.2:64955042-64955061	None:intergenic	30.0%
!	AAAACCCTTTTCAATGACTT+TGG	+	chr2.2:64954866-64954885	MS.gene96213:CDS	30.0%
!	ACATTGAACAAGCACTTTTA+AGG	+	chr2.2:64954936-64954955	MS.gene96213:CDS	30.0%
	CACTCCAAAGTCATTGAAAA+GGG	-	chr2.2:64954873-64954892	None:intergenic	35.0%
	CATGAACTTGATGAAATGAG+TGG	+	chr2.2:64954905-64954924	MS.gene96213:CDS	35.0%
	TCAAAGACCTATTAAGGTGA+AGG	-	chr2.2:64955036-64955055	None:intergenic	35.0%
	TCACTCCAAAGTCATTGAAA+AGG	-	chr2.2:64954874-64954893	None:intergenic	35.0%
	TCATCAAGTTCATGAACTTC+TGG	-	chr2.2:64954899-64954918	None:intergenic	35.0%
	TGAACTTGATGAAATGAGTG+GGG	+	chr2.2:64954907-64954926	MS.gene96213:CDS	35.0%
	TTATGAGCCTTCACCTTAAT+AGG	+	chr2.2:64955026-64955045	MS.gene96213:CDS	35.0%
	AACTTGATGAAATGAGTGGG+GGG	+	chr2.2:64954909-64954928	MS.gene96213:CDS	40.0%
	ATTCCAATGCTTAGAAGAGC+AGG	+	chr2.2:64955055-64955074	MS.gene96213:CDS	40.0%
	GAACTTGATGAAATGAGTGG+GGG	+	chr2.2:64954908-64954927	MS.gene96213:CDS	40.0%
	TAGAAGAGCAGGAGCTATTT+GGG	+	chr2.2:64955066-64955085	MS.gene96213:CDS	40.0%
	TTAGAAGAGCAGGAGCTATT+TGG	+	chr2.2:64955065-64955084	MS.gene96213:CDS	40.0%
!!!	GAACAAGCACTTTTAAGGCT+AGG	+	chr2.2:64954941-64954960	MS.gene96213:CDS	40.0%
!!	TTCATTGGTGGTGAGCTTGT+TGG	+	chr2.2:64954989-64955008	MS.gene96213:CDS	45.0%
	ATGAACACAGCAGGCACAGA+AGG	-	chr2.2:64954974-64954993	None:intergenic	50.0%
	GCTCCTGCTCTTCTAAGCAT+TGG	-	chr2.2:64955061-64955080	None:intergenic	50.0%
	TCACCACCAATGAACACAGC+AGG	-	chr2.2:64954983-64955002	None:intergenic	50.0%
	TCTGTGCCTGCTGTGTTCAT+TGG	+	chr2.2:64954974-64954993	MS.gene96213:CDS	50.0%
	TGAACACAGCAGGCACAGAA+GGG	-	chr2.2:64954973-64954992	None:intergenic	50.0%
	TGACACAGTTGGCATCAGAG+AGG	+	chr2.2:64954795-64954814	MS.gene96213:CDS	50.0%
!!	ATTGGTGGTGAGCTTGTTGG+TGG	+	chr2.2:64954992-64955011	MS.gene96213:CDS	50.0%
	GTGCCTGCTGTGTTCATTGG+TGG	+	chr2.2:64954977-64954996	MS.gene96213:CDS	55.0%
	GTTGGCATCAGAGAGGTCAG+TGG	+	chr2.2:64954802-64954821	MS.gene96213:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	64954785	64955093	64954785	ID=MS.gene96213
chr2.2	mRNA	64954785	64955093	64954785	ID=MS.gene96213.t1;Parent=MS.gene96213
chr2.2	exon	64954785	64955093	64954785	ID=MS.gene96213.t1.exon1;Parent=MS.gene96213.t1
chr2.2	CDS	64954785	64955093	64954785	ID=cds.MS.gene96213.t1;Parent=MS.gene96213.t1

Gene Sequence	>MS.gene96213 ATGGAGAGGGTGACACAGTTGGCATCAGAGAGGTCAGTGGTGATATTCAGCAAGAGTAGTTGTTGCATGTGTCACACAATCAAAACCCTTTTCAATGACTTTGGAGTGAATCCAGAAGTTCATGAACTTGATGAAATGAGTGGGGGGAGAGACATTGAACAAGCACTTTTAAGGCTAGGATGTAACCCTTCTGTGCCTGCTGTGTTCATTGGTGGTGAGCTTGTTGGTGGAGCTAATGAAGTTATGAGCCTTCACCTTAATAGGTCTTTGATTCCAATGCTTAGAAGAGCAGGAGCTATTTGGGTTTGA
Protein sequence	>MS.gene96213.t1 MERVTQLASERSVVIFSKSSCCMCHTIKTLFNDFGVNPEVHELDEMSGGRDIEQALLRLGCNPSVPAVFIGGELVGGANEVMSLHLNRSLIPMLRRAGAIWV