AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035389.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035389.01.T01 MTR_5g044895 80.102 196 35 1 5 196 1 196 3.81e-108 308
MsG0680035389.01.T01 MTR_5g072370 79.082 196 37 1 5 196 1 196 6.58e-107 305
MsG0680035389.01.T01 MTR_6g043270 77.551 196 40 1 5 196 1 196 2.12e-103 296
MsG0680035389.01.T01 MTR_3g028620 75.000 196 45 1 5 196 1 196 7.80e-103 295
MsG0680035389.01.T01 MTR_3g060830 75.000 196 45 1 5 196 1 196 1.96e-102 293
MsG0680035389.01.T01 MTR_7g061620 73.469 196 48 1 5 196 1 196 7.87e-102 292
MsG0680035389.01.T01 MTR_5g067630 75.510 196 44 1 5 196 1 196 1.81e-100 288
MsG0680035389.01.T01 MTR_7g061360 72.449 196 50 1 5 196 1 196 3.64e-100 288
MsG0680035389.01.T01 MTR_5g063995 75.510 196 44 1 5 196 1 196 5.53e-100 287
MsG0680035389.01.T01 MTR_5g009790 73.980 196 47 1 5 196 1 196 3.01e-98 288
MsG0680035389.01.T01 MTR_4g045603 71.939 196 51 1 5 196 1 196 4.83e-98 282
MsG0680035389.01.T01 MTR_8g470030 72.449 196 50 1 5 196 1 196 1.96e-96 278
MsG0680035389.01.T01 MTR_0005s0220 70.918 196 53 1 5 196 1 196 1.62e-95 276
MsG0680035389.01.T01 MTR_2g058650 69.898 196 55 1 5 196 1 196 9.22e-94 271
MsG0680035389.01.T01 MTR_0083s0070 77.143 175 36 1 26 196 1 175 3.31e-93 270
MsG0680035389.01.T01 MTR_1g036730 68.500 200 59 1 1 196 1 200 3.34e-93 270
MsG0680035389.01.T01 MTR_0360s0010 76.404 178 35 2 26 196 1 178 3.31e-91 265
MsG0680035389.01.T01 MTR_0214s0030 76.571 175 37 1 26 196 1 175 9.24e-91 263
MsG0680035389.01.T01 MTR_8g466300 74.719 178 41 1 23 196 12 189 1.07e-89 261
MsG0680035389.01.T01 MTR_5g048800 74.857 175 40 1 26 196 1 175 5.87e-89 259
MsG0680035389.01.T01 MTR_4g069290 66.000 200 60 2 5 196 1 200 2.42e-86 253
MsG0680035389.01.T01 MTR_2g061240 74.522 157 40 0 40 196 2 158 1.49e-82 242
MsG0680035389.01.T01 MTR_2g083590 60.500 200 74 2 1 196 1 199 3.10e-81 240
MsG0680035389.01.T01 MTR_5g053480 60.000 200 75 2 1 196 1 199 1.99e-78 233
MsG0680035389.01.T01 MTR_4g054700 72.258 155 43 0 42 196 9 163 1.85e-77 229
MsG0680035389.01.T01 MTR_3g033840 74.194 155 36 1 14 164 2 156 4.24e-77 228
MsG0680035389.01.T01 MTR_5g055710 79.562 137 28 0 60 196 10 146 1.37e-75 224
MsG0680035389.01.T01 MTR_7g051090 66.471 170 53 1 31 196 1 170 2.91e-75 224
MsG0680035389.01.T01 MTR_6g036940 57.895 209 70 3 1 191 1 209 3.18e-74 223
MsG0680035389.01.T01 MTR_6g042710 66.471 170 53 1 31 196 1 170 9.65e-74 220
MsG0680035389.01.T01 MTR_0520s0020 74.074 135 35 0 62 196 1 135 3.36e-69 207
MsG0680035389.01.T01 MTR_7g055890 53.000 200 86 2 5 196 1 200 1.93e-68 207
MsG0680035389.01.T01 MTR_5g080380 63.699 146 49 1 31 172 1 146 4.74e-60 184
MsG0680035389.01.T01 MTR_8g479440 78.814 118 25 0 47 164 38 155 7.45e-60 184
MsG0680035389.01.T01 MTR_7g056780 62.069 145 53 2 53 196 18 161 8.48e-57 177
MsG0680035389.01.T01 MTR_6g044850 65.833 120 41 0 77 196 1 120 1.12e-52 165
MsG0680035389.01.T01 MTR_3g117535 76.238 101 24 0 96 196 9 109 9.79e-50 157
MsG0680035389.01.T01 MTR_4g061900 57.353 136 54 1 5 136 23 158 1.84e-48 155
MsG0680035389.01.T01 MTR_1g053405 66.667 114 34 1 1 110 24 137 4.31e-48 154
MsG0680035389.01.T01 MTR_3g034005 82.222 90 16 0 107 196 1 90 9.46e-48 151
MsG0680035389.01.T01 MTR_6g466390 70.103 97 27 1 54 150 23 117 1.16e-43 143
MsG0680035389.01.T01 MTR_0134s0030 80.000 90 18 0 107 196 1 90 1.19e-43 141
MsG0680035389.01.T01 MTR_3g034000 69.697 99 26 1 5 99 1 99 5.02e-42 137
MsG0680035389.01.T01 MTR_5g084150 52.419 124 59 0 73 196 64 187 6.06e-42 140
MsG0680035389.01.T01 MTR_4g022300 60.000 110 40 1 14 119 2 111 2.01e-39 131
MsG0680035389.01.T01 MTR_8g075640 67.045 88 25 1 5 88 1 88 2.21e-35 120
MsG0680035389.01.T01 MTR_4g026610 42.857 84 36 1 59 130 1 84 2.56e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTTATGCCAAATTTA+TGG 0.109985 6:+104383897 MsG0680035389.01.T01:CDS
TGGTAGGAATTCCATAAATT+TGG 0.195147 6:-104383908 None:intergenic
ACCTCCGTTGGTTCAGATTT+GGG 0.270309 6:-104383778 None:intergenic
AACCTCCGTTGGTTCAGATT+TGG 0.272943 6:-104383779 None:intergenic
CCCTTCTTCTTCCTCTTGTT+TGG 0.286620 6:-104383928 None:intergenic
ATATCAACACTCCTCACTTT+AGG 0.299517 6:-104383751 None:intergenic
ATGAACCATGTCCTTCTCTA+AGG 0.359274 6:+104383824 MsG0680035389.01.T01:CDS
TCAAAGGAAGTTGGTTAAGA+TGG 0.389442 6:+104383693 MsG0680035389.01.T01:CDS
TCTTCTTCCTCTTGTTTGGT+AGG 0.402332 6:-104383924 None:intergenic
AAAAGTATATGTTATCTTTG+AGG 0.408804 6:+104383461 MsG0680035389.01.T01:CDS
CTCGAAATGTGATCTTTATG+AGG 0.454970 6:+104383507 MsG0680035389.01.T01:CDS
AAAGATTAACAGTGAGTGCA+TGG 0.502818 6:+104383866 MsG0680035389.01.T01:CDS
ATTATTTCTTTAACCTCCGT+TGG 0.505498 6:-104383790 None:intergenic
TTCCCTTCACAAGTACTACA+TGG 0.515262 6:-104383538 None:intergenic
CCTCCGTTGGTTCAGATTTG+GGG 0.515806 6:-104383777 None:intergenic
CAATCTCTTTAGTGTGATAG+AGG 0.523845 6:-104383423 None:intergenic
CAACCATGTAGTACTTGTGA+AGG 0.533976 6:+104383535 MsG0680035389.01.T01:CDS
AACCATGTCCTTCTCTAAGG+TGG 0.545502 6:+104383827 MsG0680035389.01.T01:CDS
ACCAAACAAGAGGAAGAAGA+AGG 0.555431 6:+104383927 MsG0680035389.01.T01:CDS
GACAAAGTGTCACAAGGTTG+TGG 0.565641 6:+104383717 MsG0680035389.01.T01:CDS
CGCCTCAACCACTGTTAGGG+TGG 0.572270 6:-104383976 None:intergenic
AAGCTCTCCCACCTTAGAGA+AGG 0.586500 6:-104383835 None:intergenic
TGGAATTCCTACCAAACAAG+AGG 0.587443 6:+104383917 MsG0680035389.01.T01:CDS
ACCATGTCCTTCTCTAAGGT+GGG 0.597882 6:+104383828 MsG0680035389.01.T01:CDS
AAGATTAACAGTGAGTGCAT+GGG 0.602478 6:+104383867 MsG0680035389.01.T01:CDS
TTTCCCCAAATCTGAACCAA+CGG 0.603568 6:+104383774 MsG0680035389.01.T01:CDS
AACCATGTAGTACTTGTGAA+GGG 0.618251 6:+104383536 MsG0680035389.01.T01:CDS
AAAGTGTCACAAGGTTGTGG+TGG 0.619032 6:+104383720 MsG0680035389.01.T01:CDS
TACTTGTGAAGGGAAGAAAG+AGG 0.633285 6:+104383546 MsG0680035389.01.T01:CDS
CCCCAAATCTGAACCAACGG+AGG 0.636208 6:+104383777 MsG0680035389.01.T01:CDS
CCAAACAAGAGGAAGAAGAA+GGG 0.638840 6:+104383928 MsG0680035389.01.T01:CDS
TGGAAGAAAATCCTAAAGTG+AGG 0.673536 6:+104383740 MsG0680035389.01.T01:CDS
GATGGAGACAAAGTGTCACA+AGG 0.686946 6:+104383711 MsG0680035389.01.T01:CDS
GAGAAGTATGATGTTCAGTG+TGG 0.713473 6:+104383643 MsG0680035389.01.T01:CDS
TCCCACCTTAGAGAAGGACA+TGG 0.722143 6:-104383829 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTATATGTTATCTTTG+AGG + Chr6:104383461-104383480 MsG0680035389.01.T01:CDS 20.0%
!!! ATCTTTGAGGATAAAAATTT+TGG + Chr6:104383474-104383493 MsG0680035389.01.T01:CDS 20.0%
! GATTCTTTATGCCAAATTTA+TGG + Chr6:104383897-104383916 MsG0680035389.01.T01:CDS 25.0%
TGGTAGGAATTCCATAAATT+TGG - Chr6:104383911-104383930 None:intergenic 30.0%
! ACATGATTTTCAAAGGAAGT+TGG + Chr6:104383684-104383703 MsG0680035389.01.T01:CDS 30.0%
! ATTATTTCTTTAACCTCCGT+TGG - Chr6:104383793-104383812 None:intergenic 30.0%
!! AGAAAGCACATGATTTTCAA+AGG + Chr6:104383677-104383696 MsG0680035389.01.T01:CDS 30.0%
AAAGATTAACAGTGAGTGCA+TGG + Chr6:104383866-104383885 MsG0680035389.01.T01:CDS 35.0%
AACCATGTAGTACTTGTGAA+GGG + Chr6:104383536-104383555 MsG0680035389.01.T01:CDS 35.0%
AAGATTAACAGTGAGTGCAT+GGG + Chr6:104383867-104383886 MsG0680035389.01.T01:CDS 35.0%
ATATCAACACTCCTCACTTT+AGG - Chr6:104383754-104383773 None:intergenic 35.0%
CAATCTCTTTAGTGTGATAG+AGG - Chr6:104383426-104383445 None:intergenic 35.0%
CTCGAAATGTGATCTTTATG+AGG + Chr6:104383507-104383526 MsG0680035389.01.T01:CDS 35.0%
TGGAAGAAAATCCTAAAGTG+AGG + Chr6:104383740-104383759 MsG0680035389.01.T01:CDS 35.0%
! TCAAAGGAAGTTGGTTAAGA+TGG + Chr6:104383693-104383712 MsG0680035389.01.T01:CDS 35.0%
ACCAAACAAGAGGAAGAAGA+AGG + Chr6:104383927-104383946 MsG0680035389.01.T01:CDS 40.0%
AGGGTGGCAAAAATGATAGA+AGG - Chr6:104383963-104383982 None:intergenic 40.0%
ATGAACCATGTCCTTCTCTA+AGG + Chr6:104383824-104383843 MsG0680035389.01.T01:CDS 40.0%
CAACCATGTAGTACTTGTGA+AGG + Chr6:104383535-104383554 MsG0680035389.01.T01:CDS 40.0%
CCAAACAAGAGGAAGAAGAA+GGG + Chr6:104383928-104383947 MsG0680035389.01.T01:CDS 40.0%
TACTTGTGAAGGGAAGAAAG+AGG + Chr6:104383546-104383565 MsG0680035389.01.T01:CDS 40.0%
TGGAATTCCTACCAAACAAG+AGG + Chr6:104383917-104383936 MsG0680035389.01.T01:CDS 40.0%
TTCCCTTCACAAGTACTACA+TGG - Chr6:104383541-104383560 None:intergenic 40.0%
TTTCCCCAAATCTGAACCAA+CGG + Chr6:104383774-104383793 MsG0680035389.01.T01:CDS 40.0%
! GAGAAGTATGATGTTCAGTG+TGG + Chr6:104383643-104383662 MsG0680035389.01.T01:CDS 40.0%
! TCTTCTTCCTCTTGTTTGGT+AGG - Chr6:104383927-104383946 None:intergenic 40.0%
CCCTTCTTCTTCCTCTTGTT+TGG - Chr6:104383931-104383950 None:intergenic 45.0%
! AAAGTGTCACAAGGTTGTGG+TGG + Chr6:104383720-104383739 MsG0680035389.01.T01:CDS 45.0%
! AACCATGTCCTTCTCTAAGG+TGG + Chr6:104383827-104383846 MsG0680035389.01.T01:CDS 45.0%
! GACAAAGTGTCACAAGGTTG+TGG + Chr6:104383717-104383736 MsG0680035389.01.T01:CDS 45.0%
! GATGGAGACAAAGTGTCACA+AGG + Chr6:104383711-104383730 MsG0680035389.01.T01:CDS 45.0%
!! AACCTCCGTTGGTTCAGATT+TGG - Chr6:104383782-104383801 None:intergenic 45.0%
!! ACCATGTCCTTCTCTAAGGT+GGG + Chr6:104383828-104383847 MsG0680035389.01.T01:CDS 45.0%
!! ACCTCCGTTGGTTCAGATTT+GGG - Chr6:104383781-104383800 None:intergenic 45.0%
AAGCTCTCCCACCTTAGAGA+AGG - Chr6:104383838-104383857 None:intergenic 50.0%
TCCCACCTTAGAGAAGGACA+TGG - Chr6:104383832-104383851 None:intergenic 50.0%
!! CCTCCGTTGGTTCAGATTTG+GGG - Chr6:104383780-104383799 None:intergenic 50.0%
CCCCAAATCTGAACCAACGG+AGG + Chr6:104383777-104383796 MsG0680035389.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 104383394 104383984 104383394 ID=MsG0680035389.01;Name=MsG0680035389.01
Chr6 mRNA 104383394 104383984 104383394 ID=MsG0680035389.01.T01;Parent=MsG0680035389.01;Name=MsG0680035389.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|196
Chr6 exon 104383394 104383984 104383394 ID=MsG0680035389.01.T01:exon:21141;Parent=MsG0680035389.01.T01
Chr6 CDS 104383394 104383984 104383394 ID=MsG0680035389.01.T01:cds;Parent=MsG0680035389.01.T01
Gene Sequence

>MsG0680035389.01.T01

ATGATAGACAGGTTAGCAGACAGATTATTCCTCTATCACACTAAAGAGATTGCTACGTTGCAGTATGAAAAGTATATGTTATCTTTGAGGATAAAAATTTTGGACGATAAAATCTCGAAATGTGATCTTTATGAGGCAAATCAACCATGTAGTACTTGTGAAGGGAAGAAAGAGGTGATCGACATAGATTACAATACTCAACTTCAAAATATTTTAGATGATTTCTTGATGACAAATCAAGTTTCATTTGAGAAGTATGATGTTCAGTGTGGTGATCTTGTTGAGAAAGCACATGATTTTCAAAGGAAGTTGGTTAAGATGGAGACAAAGTGTCACAAGGTTGTGGTGGAAGAAAATCCTAAAGTGAGGAGTGTTGATATTTTCCCCAAATCTGAACCAACGGAGGTTAAAGAAATAATTCGTGTGCAAAATGAACCATGTCCTTCTCTAAGGTGGGAGAGCTTAAATTTCAAAAGATTAACAGTGAGTGCATGGGAGTATCTGATTCTTTATGCCAAATTTATGGAATTCCTACCAAACAAGAGGAAGAAGAAGGGTGATATATTCCTTCTATCATTTTTGCCACCCTAA

Protein sequence

>MsG0680035389.01.T01

MIDRLADRLFLYHTKEIATLQYEKYMLSLRIKILDDKISKCDLYEANQPCSTCEGKKEVIDIDYNTQLQNILDDFLMTNQVSFEKYDVQCGDLVEKAHDFQRKLVKMETKCHKVVVEENPKVRSVDIFPKSEPTEVKEIIRVQNEPCPSLRWESLNFKRLTVSAWEYLILYAKFMEFLPNKRKKKGDIFLLSFLPP*