Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011056.01.T01 | XP_003597436.2 | 77.228 | 101 | 0 | 1 | 1 | 101 | 1 | 78 | 1.48E-42 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011056.01.T01 | Q8GUQ8 | 54.255 | 94 | 18 | 2 | 1 | 94 | 1 | 69 | 8.45E-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011056.01.T01 | G7IUC2 | 77.228 | 101 | 0 | 1 | 1 | 101 | 1 | 78 | 7.05e-43 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048178.01 | MsG0280011056.01 | 0.824765 | 6.761162e-54 | 6.046001e-51 |
| MsG0080048602.01 | MsG0280011056.01 | 0.827055 | 1.932821e-54 | 1.845098e-51 |
| MsG0180000146.01 | MsG0280011056.01 | 0.805194 | 1.501778e-49 | 7.895488e-47 |
| MsG0180000741.01 | MsG0280011056.01 | 0.807832 | 4.169183e-50 | 2.346067e-47 |
| MsG0180000856.01 | MsG0280011056.01 | 0.841110 | 5.796857e-58 | 8.430339e-55 |
| MsG0180001922.01 | MsG0280011056.01 | 0.837172 | 6.081601e-57 | 7.835453e-54 |
| MsG0180002071.01 | MsG0280011056.01 | 0.815228 | 1.029316e-51 | 7.055576e-49 |
| MsG0180002300.01 | MsG0280011056.01 | 0.825735 | 3.987136e-54 | 3.665134e-51 |
| MsG0180002709.01 | MsG0280011056.01 | 0.804736 | 1.872218e-49 | 9.728454e-47 |
| MsG0180003190.01 | MsG0280011056.01 | 0.820521 | 6.563308e-53 | 5.204986e-50 |
| MsG0180003303.01 | MsG0280011056.01 | 0.842839 | 2.023005e-58 | 3.105869e-55 |
| MsG0180003744.01 | MsG0280011056.01 | 0.823561 | 1.296206e-53 | 1.119940e-50 |
| MsG0180004056.01 | MsG0280011056.01 | 0.822383 | 2.439763e-53 | 2.039027e-50 |
| MsG0180004383.01 | MsG0280011056.01 | 0.803742 | 3.015371e-49 | 1.527183e-46 |
| MsG0180004444.01 | MsG0280011056.01 | 0.809765 | 1.609338e-50 | 9.531897e-48 |
| MsG0180004598.01 | MsG0280011056.01 | 0.828662 | 7.937312e-55 | 7.940834e-52 |
| MsG0180004645.01 | MsG0280011056.01 | 0.838315 | 3.093088e-57 | 4.126971e-54 |
| MsG0180004872.01 | MsG0280011056.01 | 0.807049 | 6.110687e-50 | 3.369273e-47 |
| MsG0180004986.01 | MsG0280011056.01 | 0.808914 | 2.450838e-50 | 1.418862e-47 |
| MsG0180005985.01 | MsG0280011056.01 | 0.836453 | 9.277217e-57 | 1.169700e-53 |
| MsG0180006050.01 | MsG0280011056.01 | 0.817712 | 2.860988e-52 | 2.099221e-49 |
| MsG0280006432.01 | MsG0280011056.01 | 0.842115 | 3.149554e-58 | 4.727349e-55 |
| MsG0280010735.01 | MsG0280011056.01 | 0.821597 | 3.709265e-53 | 3.031042e-50 |
| MsG0280010866.01 | MsG0280011056.01 | 0.803884 | 2.817494e-49 | 1.432217e-46 |
| MsG0280010901.01 | MsG0280011056.01 | 0.816323 | 5.866886e-52 | 4.144395e-49 |
| MsG0280010932.01 | MsG0280011056.01 | 0.804275 | 2.335495e-49 | 1.199143e-46 |
| MsG0280011055.01 | MsG0280011056.01 | 0.819287 | 1.257466e-52 | 9.635296e-50 |
| MsG0280011056.01 | MsG0280011075.01 | 0.881894 | 1.641961e-70 | 9.792272e-67 |
| MsG0280011056.01 | MsG0280011085.01 | 0.839620 | 1.420678e-57 | 1.972894e-54 |
| MsG0280011056.01 | MsG0380012124.01 | 0.803959 | 2.717263e-49 | 1.383964e-46 |
| MsG0280011056.01 | MsG0380012458.01 | 0.835212 | 1.914084e-56 | 2.324989e-53 |
| MsG0280011056.01 | MsG0380014138.01 | 0.833065 | 6.605245e-56 | 7.522976e-53 |
| MsG0280011056.01 | MsG0380015436.01 | 0.800711 | 1.267971e-48 | 5.944750e-46 |
| MsG0280011056.01 | MsG0380015607.01 | 0.822546 | 2.235555e-53 | 1.877056e-50 |
| MsG0280011056.01 | MsG0380016209.01 | 0.800999 | 1.107353e-48 | 5.230469e-46 |
| MsG0280011056.01 | MsG0380016589.01 | 0.815429 | 9.288563e-52 | 6.402430e-49 |
| MsG0280011056.01 | MsG0380017410.01 | 0.823489 | 1.347396e-53 | 1.161857e-50 |
| MsG0280011056.01 | MsG0480018332.01 | 0.809330 | 1.995839e-50 | 1.168465e-47 |
| MsG0280011056.01 | MsG0480018460.01 | 0.836295 | 1.017653e-56 | 1.277157e-53 |
| MsG0280011056.01 | MsG0480019289.01 | 0.806001 | 1.016626e-49 | 5.457140e-47 |
| MsG0280011056.01 | MsG0480019395.01 | 0.816725 | 4.768855e-52 | 3.405715e-49 |
| MsG0280011056.01 | MsG0480021134.01 | 0.820758 | 5.791750e-53 | 4.623548e-50 |
| MsG0280011056.01 | MsG0480021204.01 | 0.845253 | 4.557725e-59 | 7.550606e-56 |
| MsG0280011056.01 | MsG0480021216.01 | 0.851413 | 9.021534e-61 | 1.824650e-57 |
| MsG0280011056.01 | MsG0480021240.01 | 0.815354 | 9.652613e-52 | 6.639676e-49 |
| MsG0280011056.01 | MsG0480022398.01 | 0.801711 | 7.914884e-49 | 3.805933e-46 |
| MsG0280011056.01 | MsG0480022552.01 | 0.829645 | 4.583986e-55 | 4.720209e-52 |
| MsG0280011056.01 | MsG0480022719.01 | 0.800284 | 1.548995e-48 | 7.184673e-46 |
| MsG0280011056.01 | MsG0480023923.01 | 0.807130 | 5.873241e-50 | 3.245095e-47 |
| MsG0280011056.01 | MsG0580024205.01 | 0.826838 | 2.177299e-54 | 2.065380e-51 |
| MsG0280011056.01 | MsG0580024311.01 | 0.825067 | 5.737144e-54 | 5.174484e-51 |
| MsG0280011056.01 | MsG0580024770.01 | 0.871215 | 8.096153e-67 | 3.239488e-63 |
| MsG0280011056.01 | MsG0580024956.01 | 0.815152 | 1.069953e-51 | 7.318950e-49 |
| MsG0280011056.01 | MsG0580025128.01 | 0.849670 | 2.786110e-60 | 5.321912e-57 |
| MsG0280011056.01 | MsG0580025907.01 | 0.827555 | 1.466140e-54 | 1.420179e-51 |
| MsG0280011056.01 | MsG0580026068.01 | 0.808312 | 3.294937e-50 | 1.877760e-47 |
| MsG0280011056.01 | MsG0580029036.01 | 0.803171 | 3.958521e-49 | 1.975696e-46 |
| MsG0280011056.01 | MsG0580029658.01 | 0.805562 | 1.257565e-49 | 6.674145e-47 |
| MsG0280011056.01 | MsG0680030684.01 | 0.811218 | 7.814375e-51 | 4.809163e-48 |
| MsG0280011056.01 | MsG0680035737.01 | 0.868681 | 5.448385e-66 | 1.988320e-62 |
| MsG0280011056.01 | MsG0780035936.01 | 0.807988 | 3.862869e-50 | 2.182647e-47 |
| MsG0280011056.01 | MsG0780038888.01 | 0.818863 | 1.569935e-52 | 1.189097e-49 |
| MsG0280011056.01 | MsG0780039452.01 | 0.825176 | 5.405850e-54 | 4.891133e-51 |
| MsG0280011056.01 | MsG0780039587.01 | 0.835504 | 1.615007e-56 | 1.978823e-53 |
| MsG0280011056.01 | MsG0780040180.01 | 0.812000 | 5.284098e-51 | 3.320063e-48 |
| MsG0280011056.01 | MsG0780040687.01 | 0.809008 | 2.339810e-50 | 1.358021e-47 |
| MsG0280011056.01 | MsG0780040742.01 | 0.838209 | 3.293798e-57 | 4.380892e-54 |
| MsG0280011056.01 | MsG0780041183.01 | 0.813392 | 2.619397e-51 | 1.708534e-48 |
| MsG0280011056.01 | MsG0780041310.01 | 0.838368 | 2.998438e-57 | 4.007409e-54 |
| MsG0280011056.01 | MsG0780041370.01 | 0.855547 | 5.865428e-62 | 1.359017e-58 |
| MsG0280011056.01 | MsG0780041371.01 | 0.817567 | 3.084426e-52 | 2.254150e-49 |
| MsG0280011056.01 | MsG0780041598.01 | 0.801009 | 1.102107e-48 | 5.206997e-46 |
| MsG0280011056.01 | MsG0880042329.01 | 0.835333 | 1.783286e-56 | 2.174070e-53 |
| MsG0280011056.01 | MsG0880042783.01 | 0.811972 | 5.358199e-51 | 3.364085e-48 |
| MsG0280011056.01 | MsG0880043341.01 | 0.836228 | 1.058210e-56 | 1.325422e-53 |
| MsG0280011056.01 | MsG0880043533.01 | 0.814559 | 1.448651e-51 | 9.752426e-49 |
| MsG0280011056.01 | MsG0880044546.01 | 0.872373 | 3.342237e-67 | 1.395400e-63 |
| MsG0280011056.01 | MsG0880045165.01 | 0.857805 | 1.270873e-62 | 3.175092e-59 |
| MsG0280011056.01 | MsG0880045841.01 | 0.823917 | 1.069894e-53 | 9.338022e-51 |
| MsG0280011056.01 | MsG0880046047.01 | 0.822470 | 2.328764e-53 | 1.950966e-50 |
| MsG0280011056.01 | MsG0880046226.01 | 0.826668 | 2.391030e-54 | 2.257111e-51 |
| MsG0280011056.01 | MsG0880046404.01 | 0.814351 | 1.610655e-51 | 1.078174e-48 |
| MsG0280011056.01 | MsG0880047328.01 | -0.800101 | 1.688079e-48 | 7.793072e-46 |
| MsG0280011056.01 | MsG0880047497.01 | 0.800698 | 1.275860e-48 | 5.979831e-46 |
| MsG0280011056.01 | MsG0880047745.01 | 0.830850 | 2.327685e-55 | 2.482347e-52 |
| MsG0280006935.01 | MsG0280011056.01 | 0.800558 | 1.362040e-48 | 6.361391e-46 |
| MsG0280007011.01 | MsG0280011056.01 | 0.801387 | 9.223916e-49 | 4.399314e-46 |
| MsG0280007087.01 | MsG0280011056.01 | 0.812792 | 3.547344e-51 | 2.276413e-48 |
| MsG0280007726.01 | MsG0280011056.01 | 0.834443 | 2.988007e-56 | 3.546589e-53 |
| MsG0280007819.01 | MsG0280011056.01 | 0.817396 | 3.370880e-52 | 2.452058e-49 |
| MsG0280007829.01 | MsG0280011056.01 | 0.822026 | 2.952379e-53 | 2.442112e-50 |
| MsG0280007830.01 | MsG0280011056.01 | 0.833140 | 6.327139e-56 | 7.222348e-53 |
| MsG0280007948.01 | MsG0280011056.01 | 0.803771 | 2.972933e-49 | 1.506810e-46 |
| MsG0280008849.01 | MsG0280011056.01 | 0.810027 | 1.413542e-50 | 8.429811e-48 |
| MsG0280009386.01 | MsG0280011056.01 | 0.826475 | 2.658402e-54 | 2.495598e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011056.01.T01 | MTR_2g098030 | 77.228 | 101 | 0 | 1 | 1 | 101 | 1 | 78 | 1.79e-46 | 159 |
| MsG0280011056.01.T01 | MTR_2g098030 | 76.042 | 96 | 0 | 1 | 6 | 101 | 2 | 74 | 4.58e-43 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011056.01.T01 | AT4G34890 | 54.255 | 94 | 18 | 2 | 1 | 94 | 1 | 69 | 8.61e-13 | 63.2 |
| MsG0280011056.01.T01 | AT4G34900 | 58.667 | 75 | 8 | 1 | 20 | 94 | 10 | 61 | 2.43e-11 | 58.9 |
| MsG0280011056.01.T01 | AT4G34900 | 58.667 | 75 | 8 | 1 | 20 | 94 | 10 | 61 | 2.66e-11 | 58.9 |
Find 16 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCCGTTAACGTATAATAT+TGG | 0.300188 | 2:+78936571 | None:intergenic |
| TTGTGGAGCTTGTACTGTTA+TGG | 0.379334 | 2:-78935652 | MsG0280011056.01.T01:CDS |
| CTCATTATGATACCAATTTG+AGG | 0.434944 | 2:-78935626 | MsG0280011056.01.T01:CDS |
| TCTCCAATATTATACGTTAA+CGG | 0.443565 | 2:-78936574 | MsG0280011056.01.T01:CDS |
| GGCTGCGGTGAAGGTGGTTG+TGG | 0.472525 | 2:-78935669 | MsG0280011056.01.T01:CDS |
| CTCTTTGGTATAAAAGATAT+AGG | 0.478305 | 2:-78935711 | MsG0280011056.01.T01:CDS |
| AATGGGATCATTGAAGAAGA+TGG | 0.478328 | 2:-78936626 | MsG0280011056.01.T01:CDS |
| ATAAAAGATATAGGTCTGAC+AGG | 0.487880 | 2:-78935702 | MsG0280011056.01.T01:CDS |
| ATCATTGAAGAAGATGGATT+CGG | 0.493401 | 2:-78936620 | MsG0280011056.01.T01:CDS |
| GGTCTGACAGGGACTAAACT+AGG | 0.569006 | 2:-78935690 | MsG0280011056.01.T01:CDS |
| ACTAAACTAGGCTGCGGTGA+AGG | 0.573452 | 2:-78935678 | MsG0280011056.01.T01:CDS |
| GTGAAATGAGCTAAGTCATG+AGG | 0.605242 | 2:+78936535 | None:intergenic |
| AAACTAGGCTGCGGTGAAGG+TGG | 0.616523 | 2:-78935675 | MsG0280011056.01.T01:CDS |
| TAAAAGATATAGGTCTGACA+GGG | 0.659730 | 2:-78935701 | MsG0280011056.01.T01:CDS |
| ACAGGGACTAAACTAGGCTG+CGG | 0.662518 | 2:-78935684 | MsG0280011056.01.T01:CDS |
| ACTCTACTTGAATATCTCAG+AGG | 0.680280 | 2:-78936514 | MsG0280011056.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTCTGCTTATTTATTTA+AGG | - | Chr2:78935769-78935788 | MsG0280011056.01.T01:intron | 15.0% |
| !!! | TTTTCATATGTACTTAATTT+TGG | - | Chr2:78936450-78936469 | MsG0280011056.01.T01:intron | 15.0% |
| !! | ATAGTTTATGAAATAGCTTA+TGG | - | Chr2:78936010-78936029 | MsG0280011056.01.T01:intron | 20.0% |
| !!! | CTTTAACTTAAGCTAAAAAT+TGG | + | Chr2:78936304-78936323 | None:intergenic | 20.0% |
| ! | AATAAGCTGAAAATAGCTTA+TGG | + | Chr2:78936163-78936182 | None:intergenic | 25.0% |
| ! | ATTCCGTTAACGTATAATAT+TGG | + | Chr2:78935671-78935690 | None:intergenic | 25.0% |
| ! | CATAAACTATCTTGAACTTA+TGG | + | Chr2:78936000-78936019 | None:intergenic | 25.0% |
| ! | CTCTTTGGTATAAAAGATAT+AGG | - | Chr2:78936528-78936547 | MsG0280011056.01.T01:CDS | 25.0% |
| ! | TAGCTTAAGTTAAAGTCATT+TGG | - | Chr2:78936309-78936328 | MsG0280011056.01.T01:intron | 25.0% |
| ! | TCTCCAATATTATACGTTAA+CGG | - | Chr2:78935665-78935684 | MsG0280011056.01.T01:CDS | 25.0% |
| AGTTAGTTGTTTATCCAAAC+AGG | - | Chr2:78936130-78936149 | MsG0280011056.01.T01:intron | 30.0% | |
| ATAAAAGATATAGGTCTGAC+AGG | - | Chr2:78936537-78936556 | MsG0280011056.01.T01:CDS | 30.0% | |
| CTCATTATGATACCAATTTG+AGG | - | Chr2:78936613-78936632 | MsG0280011056.01.T01:CDS | 30.0% | |
| TAAAAGATATAGGTCTGACA+GGG | - | Chr2:78936538-78936557 | MsG0280011056.01.T01:CDS | 30.0% | |
| ! | AATCATGAAGTGCTTATGTA+GGG | + | Chr2:78936414-78936433 | None:intergenic | 30.0% |
| ! | ATCATTGAAGAAGATGGATT+CGG | - | Chr2:78935619-78935638 | MsG0280011056.01.T01:CDS | 30.0% |
| ! | ATCTTTTATACCAAAGAGAG+TGG | + | Chr2:78936526-78936545 | None:intergenic | 30.0% |
| ! | TAATCAGTTTAGCTTCACAA+AGG | + | Chr2:78936496-78936515 | None:intergenic | 30.0% |
| ! | TAATCATGAAGTGCTTATGT+AGG | + | Chr2:78936415-78936434 | None:intergenic | 30.0% |
| ! | TTATAGTTTTCCACTCTCTT+TGG | - | Chr2:78936513-78936532 | MsG0280011056.01.T01:CDS | 30.0% |
| !! | AACTAATTTGAGTTGTGGTT+TGG | - | Chr2:78936377-78936396 | MsG0280011056.01.T01:intron | 30.0% |
| !! | GACAATTAACTTGCTGAAAT+TGG | + | Chr2:78936343-78936362 | None:intergenic | 30.0% |
| !! | TCTTGAACTAATTTGAGTTG+TGG | - | Chr2:78936372-78936391 | MsG0280011056.01.T01:intron | 30.0% |
| ACTCTACTTGAATATCTCAG+AGG | - | Chr2:78935725-78935744 | MsG0280011056.01.T01:intron | 35.0% | |
| CTCGTATAAGTCAATTCAGA+CGG | + | Chr2:78935903-78935922 | None:intergenic | 35.0% | |
| GAAATAGCTTATGGCTTATG+CGG | - | Chr2:78936019-78936038 | MsG0280011056.01.T01:intron | 35.0% | |
| TACTACTTGTGAGAACGTTT+GGG | - | Chr2:78935848-78935867 | MsG0280011056.01.T01:intron | 35.0% | |
| TTACTACTTGTGAGAACGTT+TGG | - | Chr2:78935847-78935866 | MsG0280011056.01.T01:intron | 35.0% | |
| ! | TCACAAGGTTTTCCTCAAAT+TGG | + | Chr2:78936628-78936647 | None:intergenic | 35.0% |
| !! | TTGGTCTTACTAAGCACTTA+CGG | + | Chr2:78936285-78936304 | None:intergenic | 35.0% |
| CTTATGGACATAGTCCTGTT+TGG | + | Chr2:78936147-78936166 | None:intergenic | 40.0% | |
| GTGAAATGAGCTAAGTCATG+AGG | + | Chr2:78935707-78935726 | None:intergenic | 40.0% | |
| TACCACTTGTGAGACTGTTT+AGG | - | Chr2:78935934-78935953 | MsG0280011056.01.T01:intron | 40.0% | |
| TTGTGGAGCTTGTACTGTTA+TGG | - | Chr2:78936587-78936606 | MsG0280011056.01.T01:CDS | 40.0% | |
| CTCCTAAACAGTCTCACAAG+TGG | + | Chr2:78935939-78935958 | None:intergenic | 45.0% | |
| ACAGGGACTAAACTAGGCTG+CGG | - | Chr2:78936555-78936574 | MsG0280011056.01.T01:CDS | 50.0% | |
| ACTAAACTAGGCTGCGGTGA+AGG | - | Chr2:78936561-78936580 | MsG0280011056.01.T01:CDS | 50.0% | |
| GGTCTGACAGGGACTAAACT+AGG | - | Chr2:78936549-78936568 | MsG0280011056.01.T01:CDS | 50.0% | |
| AAACTAGGCTGCGGTGAAGG+TGG | - | Chr2:78936564-78936583 | MsG0280011056.01.T01:CDS | 55.0% | |
| GGCTGCGGTGAAGGTGGTTG+TGG | - | Chr2:78936570-78936589 | MsG0280011056.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 78935614 | 78936647 | 78935614 | ID=MsG0280011056.01;Name=MsG0280011056.01 |
| Chr2 | mRNA | 78935614 | 78936647 | 78935614 | ID=MsG0280011056.01.T01;Parent=MsG0280011056.01;Name=MsG0280011056.01.T01;_AED=0.52;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|101 |
| Chr2 | exon | 78936471 | 78936647 | 78936471 | ID=MsG0280011056.01.T01:exon:17428;Parent=MsG0280011056.01.T01 |
| Chr2 | exon | 78935614 | 78935742 | 78935614 | ID=MsG0280011056.01.T01:exon:17427;Parent=MsG0280011056.01.T01 |
| Chr2 | CDS | 78936471 | 78936647 | 78936471 | ID=MsG0280011056.01.T01:cds;Parent=MsG0280011056.01.T01 |
| Chr2 | CDS | 78935614 | 78935742 | 78935614 | ID=MsG0280011056.01.T01:cds;Parent=MsG0280011056.01.T01 |
| Gene Sequence |
| Protein sequence |
