AlfalfaGEDB | MsG0380014239.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014239.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014239.01.T01	XP_039687102.1	90.441	136	13	0	1	136	712	847	6.79E-75	250

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014239.01.T01	A0A396IRN9	90.441	136	13	0	1	136	712	847	3.24e-75	250

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048874.01	MsG0380014239.01	0.803614	3.205186e-49	1.617989e-46
MsG0180000145.01	MsG0380014239.01	0.800829	1.199367e-48	5.640238e-46
MsG0180000470.01	MsG0380014239.01	0.844748	6.236538e-59	1.016758e-55
MsG0180001884.01	MsG0380014239.01	0.802701	4.951522e-49	2.441856e-46
MsG0180001971.01	MsG0380014239.01	0.825177	5.404277e-54	4.889768e-51
MsG0180002282.01	MsG0380014239.01	0.800952	1.132137e-48	5.340913e-46
MsG0180002842.01	MsG0380014239.01	0.840785	7.052033e-58	1.015403e-54
MsG0180003673.01	MsG0380014239.01	0.818810	1.613921e-52	1.220511e-49
MsG0180003686.01	MsG0380014239.01	0.821438	4.037189e-53	3.284474e-50
MsG0180004264.01	MsG0380014239.01	0.806408	8.344529e-50	4.525659e-47
MsG0180005050.01	MsG0380014239.01	0.814261	1.685889e-51	1.125809e-48
MsG0180005185.01	MsG0380014239.01	0.831879	1.299764e-55	1.429204e-52
MsG0180005705.01	MsG0380014239.01	0.804511	2.085313e-49	1.077183e-46
MsG0280006325.01	MsG0380014239.01	0.803001	4.292683e-49	2.133047e-46
MsG0280010698.01	MsG0380014239.01	0.811991	5.307559e-51	3.333994e-48
MsG0280011032.01	MsG0380014239.01	0.804047	2.604959e-49	1.329743e-46
MsG0380011494.01	MsG0380014239.01	0.818732	1.681233e-52	1.268693e-49
MsG0380011609.01	MsG0380014239.01	0.814158	1.776064e-51	1.182719e-48
MsG0380012009.01	MsG0380014239.01	0.803514	3.362111e-49	1.692895e-46
MsG0380012085.01	MsG0380014239.01	0.802468	5.531317e-49	2.711582e-46
MsG0380012330.01	MsG0380014239.01	0.803735	3.024974e-49	1.531777e-46
MsG0380012460.01	MsG0380014239.01	0.811492	6.815567e-51	4.224973e-48
MsG0380014239.01	MsG0380014251.01	0.811608	6.429203e-51	3.997477e-48
MsG0380014239.01	MsG0380014468.01	0.800853	1.185939e-48	5.580724e-46
MsG0380014239.01	MsG0380014573.01	0.821596	3.712665e-53	3.033682e-50
MsG0380014239.01	MsG0380015034.01	0.808069	3.711072e-50	2.101374e-47
MsG0380014239.01	MsG0380015449.01	0.807397	5.156425e-50	2.869092e-47
MsG0380014239.01	MsG0380015566.01	0.810604	1.061170e-50	6.425096e-48
MsG0380014239.01	MsG0380015720.01	0.803683	3.101224e-49	1.568247e-46
MsG0380014239.01	MsG0380016064.01	0.810287	1.242442e-50	7.459284e-48
MsG0380014239.01	MsG0380016910.01	0.817860	2.649193e-52	1.951732e-49
MsG0380014239.01	MsG0380017048.01	0.802111	6.550411e-49	3.182042e-46
MsG0380014239.01	MsG0380017220.01	0.801061	1.075397e-48	5.087371e-46
MsG0380014239.01	MsG0480018163.01	0.804769	1.842178e-49	9.580276e-47
MsG0380014239.01	MsG0480018183.01	0.808823	2.563236e-50	1.480394e-47
MsG0380014239.01	MsG0480018236.01	0.803552	3.300623e-49	1.663613e-46
MsG0380014239.01	MsG0480018465.01	0.801868	7.349657e-49	3.548476e-46
MsG0380014239.01	MsG0480018576.01	0.807003	6.249271e-50	3.441576e-47
MsG0380014239.01	MsG0480019297.01	0.807019	6.200482e-50	3.416168e-47
MsG0380014239.01	MsG0480019970.01	0.803528	3.339602e-49	1.682195e-46
MsG0380014239.01	MsG0480020634.01	0.807001	6.256186e-50	3.445178e-47
MsG0380014239.01	MsG0480021688.01	0.815155	1.068668e-51	7.310646e-49
MsG0380014239.01	MsG0480021784.01	0.802666	5.035253e-49	2.480763e-46
MsG0380014239.01	MsG0480021853.01	0.825826	3.792944e-54	3.495708e-51
MsG0380014239.01	MsG0480021925.01	0.848517	5.827201e-60	1.072221e-56
MsG0380014239.01	MsG0480022068.01	0.822695	2.064699e-53	1.740853e-50
MsG0380014239.01	MsG0480022259.01	0.810598	1.064503e-50	6.444165e-48
MsG0380014239.01	MsG0480022262.01	0.818883	1.553366e-52	1.177194e-49
MsG0380014239.01	MsG0480022615.01	-0.801427	9.052338e-49	4.321801e-46
MsG0380014239.01	MsG0580024038.01	0.819365	1.206976e-52	9.268312e-50
MsG0380014239.01	MsG0580024552.01	0.845176	4.782120e-59	7.902306e-56
MsG0380014239.01	MsG0580024690.01	0.802355	5.836746e-49	2.853184e-46
MsG0380014239.01	MsG0580025022.01	0.803497	3.388812e-49	1.705593e-46
MsG0380014239.01	MsG0580025638.01	0.800130	1.665322e-48	7.693747e-46
MsG0380014239.01	MsG0580025758.01	0.813432	2.567956e-51	1.676714e-48
MsG0380014239.01	MsG0580027018.01	0.848209	7.089187e-60	1.291247e-56
MsG0380014239.01	MsG0580027745.01	0.800274	1.556183e-48	7.215977e-46
MsG0380014239.01	MsG0580027767.01	0.803912	2.779021e-49	1.413744e-46
MsG0380014239.01	MsG0580027833.01	0.813854	2.072865e-51	1.368993e-48
MsG0380014239.01	MsG0580027922.01	0.807095	5.975050e-50	3.298395e-47
MsG0380014239.01	MsG0580027926.01	0.808256	3.386123e-50	1.926981e-47
MsG0380014239.01	MsG0580029622.01	0.800118	1.674241e-48	7.732607e-46
MsG0380014239.01	MsG0680031262.01	0.802895	4.515004e-49	2.237615e-46
MsG0380014239.01	MsG0680031379.01	0.821531	3.842650e-53	3.134225e-50
MsG0380014239.01	MsG0680032158.01	0.814038	1.888012e-51	1.253189e-48
MsG0380014239.01	MsG0680033412.01	0.800769	1.234045e-48	5.794212e-46
MsG0380014239.01	MsG0680034464.01	0.813379	2.637738e-51	1.719835e-48
MsG0380014239.01	MsG0680035573.01	0.804485	2.112331e-49	1.090407e-46
MsG0380014239.01	MsG0680035692.01	0.813619	2.334811e-51	1.532287e-48
MsG0380014239.01	MsG0780036018.01	0.845530	3.834873e-59	6.409656e-56
MsG0380014239.01	MsG0780036080.01	0.806478	8.065825e-50	4.382450e-47
MsG0380014239.01	MsG0780037592.01	0.812597	3.913532e-51	2.498727e-48
MsG0380014239.01	MsG0780037845.01	0.845160	4.827943e-59	7.973984e-56
MsG0380014239.01	MsG0780038391.01	0.804432	2.166761e-49	1.116981e-46
MsG0380014239.01	MsG0780038401.01	0.801265	9.767843e-49	4.644508e-46
MsG0380014239.01	MsG0780038602.01	0.820154	7.969397e-53	6.256615e-50
MsG0380014239.01	MsG0780039252.01	0.803046	4.201414e-49	2.090137e-46
MsG0380014239.01	MsG0780040576.01	-0.803791	2.944620e-49	1.493263e-46
MsG0380014239.01	MsG0780040596.01	0.818440	1.957775e-52	1.465700e-49
MsG0380014239.01	MsG0780040704.01	0.820953	5.223304e-53	4.192909e-50
MsG0380014239.01	MsG0780041456.01	0.812813	3.510498e-51	2.253970e-48
MsG0380014239.01	MsG0880042161.01	0.813553	2.415354e-51	1.582279e-48
MsG0380014239.01	MsG0880043941.01	0.812539	4.028770e-51	2.568532e-48
MsG0380014239.01	MsG0880044574.01	0.832475	9.257317e-56	1.035835e-52
MsG0380014239.01	MsG0880045111.01	0.816904	4.347337e-52	3.120002e-49
MsG0380014239.01	MsG0880045372.01	0.803707	3.065933e-49	1.551325e-46
MsG0380014239.01	MsG0880045736.01	0.809068	2.271656e-50	1.320626e-47
MsG0380014239.01	MsG0880045895.01	0.831523	1.590317e-55	1.730210e-52
MsG0380014239.01	MsG0880045937.01	0.806784	6.953489e-50	3.807686e-47
MsG0380014239.01	MsG0880046020.01	0.833965	3.938389e-56	4.608273e-53
MsG0380014239.01	MsG0880046425.01	0.812993	3.205351e-51	2.068089e-48
MsG0380014239.01	MsG0880046479.01	0.805407	1.354832e-49	7.162181e-47
MsG0280006770.01	MsG0380014239.01	0.837247	5.816626e-57	7.510731e-54
MsG0280007066.01	MsG0380014239.01	0.803878	2.824643e-49	1.435672e-46
MsG0280007072.01	MsG0380014239.01	0.803101	4.094364e-49	2.039654e-46
MsG0280008524.01	MsG0380014239.01	0.818801	1.621385e-52	1.225856e-49
MsG0280008894.01	MsG0380014239.01	0.807994	3.851030e-50	2.176300e-47
MsG0280010443.01	MsG0380014239.01	0.848227	7.008233e-60	1.277306e-56

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014239.01.T01	MTR_3g053340	90.441	136	13	1	136	648	783	1.11e-78	248
MsG0380014239.01.T01	MTR_5g096500	90.441	136	13	1	136	702	837	1.58e-77	246
MsG0380014239.01.T01	MTR_5g096500	90.441	136	13	1	136	703	838	1.65e-77	246

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAGTTTCACTGCTGAATA+AGG	0.267921	3:+51737769	MsG0380014239.01.T01:CDS
TTGCCTCAGTCGGTCAATTT+TGG	0.288627	3:+51737734	MsG0380014239.01.T01:CDS
CATTCAGAAGGTCTGGTATC+TGG	0.302489	3:+51738053	MsG0380014239.01.T01:CDS
ATCGGTTCAAGAGGTAAATA+AGG	0.366054	3:+51737958	MsG0380014239.01.T01:CDS
GGAGTCAACTTTCTTTCTTT+CGG	0.379336	3:+51737647	MsG0380014239.01.T01:CDS
CTGCTGAATAAGGATGATAA+TGG	0.386959	3:+51737779	MsG0380014239.01.T01:CDS
AGTTGAAGCCTGAAAATTAT+CGG	0.396775	3:-51737984	None:intergenic
AACTTTCTTTCTTTCGGCAA+AGG	0.445900	3:+51737653	MsG0380014239.01.T01:CDS
CATCTGTCATTCAGAAGGTC+TGG	0.454603	3:+51738046	MsG0380014239.01.T01:CDS
TGTGAGAATAGCGCAGTTTG+TGG	0.460073	3:-51737833	None:intergenic
AGAAAGAAAGTTGACTCCCT+TGG	0.474127	3:-51737642	None:intergenic
TCAGTCGTTCTGCCATGGAT+TGG	0.481190	3:-51737874	None:intergenic
GAAAGAAAGTTGACTCCCTT+GGG	0.483104	3:-51737641	None:intergenic
AGATCACAGCCATTGTAAAT+CGG	0.483854	3:+51737940	MsG0380014239.01.T01:CDS
TGATTTCAGTCGTTCTGCCA+TGG	0.505856	3:-51737879	None:intergenic
TTTCACATCTGTCATTCAGA+AGG	0.530662	3:+51738041	MsG0380014239.01.T01:CDS
AATGACAGATGTGAAACAAA+GGG	0.544236	3:-51738034	None:intergenic
CCTCTTGAACCGATTTACAA+TGG	0.556834	3:-51737949	None:intergenic
CAATATCATTCCTTCTGCAG+TGG	0.568846	3:-51737708	None:intergenic
ACTAGCAAGAAGCGTAGCTG+AGG	0.573123	3:-51738011	None:intergenic
TCTATTCACTCCACTGCAGA+AGG	0.576435	3:+51737698	MsG0380014239.01.T01:CDS
TATTCCAGCTGTCATGCCCA+AGG	0.588364	3:+51737625	None:intergenic
GAATGACAGATGTGAAACAA+AGG	0.596112	3:-51738035	None:intergenic
CCATTGTAAATCGGTTCAAG+AGG	0.603158	3:+51737949	MsG0380014239.01.T01:CDS
AAACCAAAATTGACCGACTG+AGG	0.618079	3:-51737737	None:intergenic
ATTCCAGCTGTCATGCCCAA+GGG	0.640386	3:+51737626	None:intergenic
GATATTGAAGTTGCCTCAGT+CGG	0.657270	3:+51737724	MsG0380014239.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GACTGAAATCAAGATTTTTA+GGG	+	Chr3:51737891-51737910	MsG0380014239.01.T01:CDS	25.0%
	AATGACAGATGTGAAACAAA+GGG	-	Chr3:51738037-51738056	None:intergenic	30.0%
	AGTTGAAGCCTGAAAATTAT+CGG	-	Chr3:51737987-51738006	None:intergenic	30.0%
!	CGACTGAAATCAAGATTTTT+AGG	+	Chr3:51737890-51737909	MsG0380014239.01.T01:CDS	30.0%
	AACTTTCTTTCTTTCGGCAA+AGG	+	Chr3:51737653-51737672	MsG0380014239.01.T01:CDS	35.0%
	AGATCACAGCCATTGTAAAT+CGG	+	Chr3:51737940-51737959	MsG0380014239.01.T01:CDS	35.0%
	CTGCTGAATAAGGATGATAA+TGG	+	Chr3:51737779-51737798	MsG0380014239.01.T01:CDS	35.0%
	GAATGACAGATGTGAAACAA+AGG	-	Chr3:51738038-51738057	None:intergenic	35.0%
	GGAGTCAACTTTCTTTCTTT+CGG	+	Chr3:51737647-51737666	MsG0380014239.01.T01:CDS	35.0%
	TTCAGACAAAAACCAATCCA+TGG	+	Chr3:51737862-51737881	MsG0380014239.01.T01:CDS	35.0%
	TTCAGTTTCACTGCTGAATA+AGG	+	Chr3:51737769-51737788	MsG0380014239.01.T01:CDS	35.0%
	TTTCACATCTGTCATTCAGA+AGG	+	Chr3:51738041-51738060	MsG0380014239.01.T01:CDS	35.0%
!	ATCGGTTCAAGAGGTAAATA+AGG	+	Chr3:51737958-51737977	MsG0380014239.01.T01:CDS	35.0%
!	ATTTTGTGCAGTTCTATCAC+TGG	-	Chr3:51737810-51737829	None:intergenic	35.0%
!!	TAAGGCATCCGATAATTTTC+AGG	+	Chr3:51737976-51737995	MsG0380014239.01.T01:CDS	35.0%
	AAACCAAAATTGACCGACTG+AGG	-	Chr3:51737740-51737759	None:intergenic	40.0%
	CAATATCATTCCTTCTGCAG+TGG	-	Chr3:51737711-51737730	None:intergenic	40.0%
	GATATTGAAGTTGCCTCAGT+CGG	+	Chr3:51737724-51737743	MsG0380014239.01.T01:CDS	40.0%
!	AGAAAGAAAGTTGACTCCCT+TGG	-	Chr3:51737645-51737664	None:intergenic	40.0%
!	CCATTGTAAATCGGTTCAAG+AGG	+	Chr3:51737949-51737968	MsG0380014239.01.T01:CDS	40.0%
!	CCTCTTGAACCGATTTACAA+TGG	-	Chr3:51737952-51737971	None:intergenic	40.0%
!	GAAAGAAAGTTGACTCCCTT+GGG	-	Chr3:51737644-51737663	None:intergenic	40.0%
	CATCTGTCATTCAGAAGGTC+TGG	+	Chr3:51738046-51738065	MsG0380014239.01.T01:CDS	45.0%
	CATTCAGAAGGTCTGGTATC+TGG	+	Chr3:51738053-51738072	MsG0380014239.01.T01:CDS	45.0%
	TCTATTCACTCCACTGCAGA+AGG	+	Chr3:51737698-51737717	MsG0380014239.01.T01:CDS	45.0%
	TGATTTCAGTCGTTCTGCCA+TGG	-	Chr3:51737882-51737901	None:intergenic	45.0%
	TGTGAGAATAGCGCAGTTTG+TGG	-	Chr3:51737836-51737855	None:intergenic	45.0%
!	TTGCCTCAGTCGGTCAATTT+TGG	+	Chr3:51737734-51737753	MsG0380014239.01.T01:CDS	45.0%
	TCAGTCGTTCTGCCATGGAT+TGG	-	Chr3:51737877-51737896	None:intergenic	50.0%
!	ACTAGCAAGAAGCGTAGCTG+AGG	-	Chr3:51738014-51738033	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	51737638	51738084	51737638	ID=MsG0380014239.01;Name=MsG0380014239.01
Chr3	mRNA	51737638	51738084	51737638	ID=MsG0380014239.01.T01;Parent=MsG0380014239.01;Name=MsG0380014239.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr3	exon	51737638	51738084	51737638	ID=MsG0380014239.01.T01:exon:10064;Parent=MsG0380014239.01.T01
Chr3	CDS	51737638	51738084	51737638	ID=MsG0380014239.01.T01:cds;Parent=MsG0380014239.01.T01

Gene Sequence

>MsG0380014239.01.T01

ATGCCCAAGGGAGTCAACTTTCTTTCTTTCGGCAAAGGTTCTGTTTGCAGTTTTAAACGTTCTATTCACTCCACTGCAGAAGGAATGATATTGAAGTTGCCTCAGTCGGTCAATTTTGGTTTGTCTGAACATTCAGTTTCACTGCTGAATAAGGATGATAATGGAAAAACCAGTGATAGAACTGCACAAAATTTACCACAAACTGCGCTATTCTCACATGAGATTTCAGACAAAAACCAATCCATGGCAGAACGACTGAAATCAAGATTTTTAGGGAAAGATTCAGCTGATGATTCTGTGAGAGATCACAGCCATTGTAAATCGGTTCAAGAGGTAAATAAGGCATCCGATAATTTTCAGGCTTCAACTGTGACCTCAGCTACGCTTCTTGCTAGTCCCTTTGTTTCACATCTGTCATTCAGAAGGTCTGGTATCTGGATGTCATAA

Protein sequence

>MsG0380014239.01.T01

MPKGVNFLSFGKGSVCSFKRSIHSTAEGMILKLPQSVNFGLSEHSVSLLNKDDNGKTSDRTAQNLPQTALFSHEISDKNQSMAERLKSRFLGKDSADDSVRDHSHCKSVQEVNKASDNFQASTVTSATLLASPFVSHLSFRRSGIWMS*