Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022393.01.T01 | BBA20646.1 | 41.083 | 314 | 184 | 1 | 1 | 314 | 1 | 313 | 1.94E-79 | 264 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022393.01.T01 | A0A224AUQ0 | 41.083 | 314 | 184 | 1 | 1 | 314 | 1 | 313 | 9.26e-80 | 264 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048809.01 | MsG0480022393.01 | 0.856205 | 3.764709e-62 | 8.915989e-59 |
| MsG0180001312.01 | MsG0480022393.01 | -0.806646 | 7.436436e-50 | 4.057460e-47 |
| MsG0180002140.01 | MsG0480022393.01 | -0.811342 | 7.344516e-51 | 4.534515e-48 |
| MsG0180002955.01 | MsG0480022393.01 | -0.816695 | 4.844346e-52 | 3.456705e-49 |
| MsG0180003118.01 | MsG0480022393.01 | 0.834574 | 2.770311e-56 | 3.301285e-53 |
| MsG0180003357.01 | MsG0480022393.01 | -0.818356 | 2.045715e-52 | 1.527924e-49 |
| MsG0180004684.01 | MsG0480022393.01 | -0.820217 | 7.709520e-53 | 6.062792e-50 |
| MsG0180005106.01 | MsG0480022393.01 | -0.817158 | 3.811769e-52 | 2.754792e-49 |
| MsG0180005160.01 | MsG0480022393.01 | -0.837055 | 6.512734e-57 | 8.361160e-54 |
| MsG0180005354.01 | MsG0480022393.01 | -0.872340 | 3.428297e-67 | 1.429329e-63 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022393.01.T01 | MTR_4g088850 | 100.000 | 37 | 0 | 0 | 449 | 485 | 75 | 111 | 2.93e-20 | 87.0 |
| MsG0480022393.01.T01 | MTR_4g088850 | 100.000 | 37 | 0 | 0 | 449 | 485 | 75 | 111 | 4.48e-20 | 87.0 |
Find 139 sgRNAs with CRISPR-Local
Find 173 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGTGGTTAAGTTCCAT+TGG | 0.471407 | 4:+72007447 | MsG0480022393.01.T01:CDS |
| AAAGTTGAAGATTACATGGG+AGG | 0.630761 | 4:+72008135 | MsG0480022393.01.T01:CDS |
| AACAACTATTTCAACATCGA+TGG | 0.502661 | 4:+72007752 | MsG0480022393.01.T01:CDS |
| AACAACTCGTTGATCATAGT+TGG | 0.492115 | 4:-72008104 | None:intergenic |
| AACCTGATCAGCTGTGACGA+AGG | 0.593008 | 4:-72009136 | None:intergenic |
| AACGGCTTGTCCTGAGCCTT+CGG | 0.431060 | 4:-72008278 | None:intergenic |
| AACTATGATCAACGAGTTGT+TGG | 0.470840 | 4:+72008106 | MsG0480022393.01.T01:CDS |
| AACTGGGGTGTTACCTTCAG+GGG | 0.576734 | 4:-72007216 | None:intergenic |
| AAGGACGGAGGTCTGCTGGC+TGG | 0.507322 | 4:-72007248 | None:intergenic |
| AAGTTAGGTGTTCATCATGC+CGG | 0.527912 | 4:+72009217 | MsG0480022393.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAACTATTTCAACATCGA+TGG | + | Chr4:72007752-72007771 | MsG0480022393.01.T01:CDS | 30.0% | |
| AACTTCCTGGTCATTATAAA+AGG | + | Chr4:72008869-72008888 | MsG0480022393.01.T01:intron | 30.0% | |
| ACTTCCTGGTCATTATAAAA+GGG | + | Chr4:72008870-72008889 | MsG0480022393.01.T01:intron | 30.0% | |
| AGCTAACATGTATAAGATCA+TGG | + | Chr4:72008408-72008427 | MsG0480022393.01.T01:CDS | 30.0% | |
| ATTTGTCCAAAGTACAAACA+AGG | - | Chr4:72007577-72007596 | None:intergenic | 30.0% | |
| CAGGAAATTTGATTACAATC+AGG | - | Chr4:72008538-72008557 | None:intergenic | 30.0% | |
| CGATTCAGTTCAATAAATTG+GGG | - | Chr4:72007359-72007378 | None:intergenic | 30.0% | |
| CTCAAGTTTATATCTCACAT+TGG | + | Chr4:72007504-72007523 | MsG0480022393.01.T01:CDS | 30.0% | |
| GCGATTCAGTTCAATAAATT+GGG | - | Chr4:72007360-72007379 | None:intergenic | 30.0% | |
| TAGTTGATCTACGAAGATTT+GGG | - | Chr4:72007288-72007307 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 72007191 | 72009311 | 72007191 | ID=MsG0480022393.01;Name=MsG0480022393.01 |
| Chr4 | mRNA | 72007191 | 72009311 | 72007191 | ID=MsG0480022393.01.T01;Parent=MsG0480022393.01;Name=MsG0480022393.01.T01;_AED=0.49;_eAED=0.96;_QI=0|0|0|1|0|0|2|0|502 |
| Chr4 | exon | 72007191 | 72008450 | 72007191 | ID=MsG0480022393.01.T01:exon:4998;Parent=MsG0480022393.01.T01 |
| Chr4 | exon | 72009063 | 72009311 | 72009063 | ID=MsG0480022393.01.T01:exon:4999;Parent=MsG0480022393.01.T01 |
| Chr4 | CDS | 72007191 | 72008450 | 72007191 | ID=MsG0480022393.01.T01:cds;Parent=MsG0480022393.01.T01 |
| Chr4 | CDS | 72009063 | 72009311 | 72009063 | ID=MsG0480022393.01.T01:cds;Parent=MsG0480022393.01.T01 |
| Gene Sequence |
>MsG0480022393.01.T01 ATGTCAGGACTGGATGAAGATACGACCCCTGAAGGTAACACCCCAGTTATCAAAGCTCCAGCCAGCAGACCTCCGTCCTTTTCAAGGACCACTACCCAAATCTTCGTAGATCAACTACAACAGATGAACCATATCGGATCATTCACCATTCTTGGACCCAACAATCCCCAATTTATTGAACTGAATCGCAATGAAATTTTCAATGCTCTACGGGACCTTTACACCCTCCACTTTATGTCAAAGTGGGACATTTTGAAAAGAGTGGTTAAGTTCCATTGGAACTCCACCACTGCTGAGGAGGCTAAATACCTCTCTCAAGTTTATATCTCACATTGGTTCTTAGATGTTTATGCCTCAAACAGAGAGGCTTGCAGGAAGCTTCCCCTTGTTTGTACTTTGGACAAATTTGCAGTTGAGATAGGTATTCAATCAACAATTTATGATAATTACCTCACCCTATTGCTCGATGCTCTCAAGCCGACTCGGGTCACTGGAACGCCTGACAGTTCCATTCTCATTCCAATCATTTGCACCACCCCATTTTGGAACCACCTAGACGGTAACAACTATTTCAACATCGATGGATTTATCCTCAATACTGAACTCTTCAGCGCACTGATGCAGGTCATGACTGACCCAAACAGCAAATGGCTGATGAACACCTATTCCAATGACGTTCTAGGACGGCCATTTTGGCTACTTGACTGGCATAATGACCAAATATTTTCTTGGTTCCCAGAAGATGAGAACTATAACCAGGATGACATCAACCTGGCCCTAATTTTAGGAGTAGCCTGTACTCCACACCTAGCCCCAAGCGATGATGATATCCCACAGCAATATCCAAACAATGTCGTGCCTAACGCCGACACAATTTTCAAATATGAACGAAGAATTCCCGCTCAGTTCTACTCCAACTATGATCAACGAGTTGTTGGTCATCAAAAGTTGAAGATTACATGGGAGGAAATGCTAACCCATAATGAAATTGAAGCTGCAAGACAGAAGAATGAAGAAGAATACAATATCCAGCTTAAAGCTGAGTATACAAAGCTCGTCAACTCAAGACCTGCTGCAATGGATACAGCCGAAGGCTCAGGACAAGCCGTTGTCCCATTTCGCGCCACTGGCGAAATGATTGACCAAGAACTCATGGCTCATGCCAAGACGAAGATCAAGATGATAGACCCGCCATTGACAAAAGAGGATAGTTTTGTAGCTAACATGTATAAGATCATGGACTATAAGTACTACGCTCATTTCACTATACTTGTACAAAATATGACATGGCATTATTCTTTTGCATGGCAAGCTACTTATATCTTAACTCATTCCTTCGTCACAGCTGATCAGGTTGGACAAGTTAAGTGTGGGAGTTGTGCAGTATTGCTAATGTACCCATACGGTGCTCCACAAGTTAGGTGTTCATCATGCCGGTTTGTGACAGAAATCGGGGTATGTAAACTGTTGACATTCACTAGTTCTCTTTATACATGTTTTACCTTCTAA |
| Protein sequence |
>MsG0480022393.01.T01 MSGLDEDTTPEGNTPVIKAPASRPPSFSRTTTQIFVDQLQQMNHIGSFTILGPNNPQFIELNRNEIFNALRDLYTLHFMSKWDILKRVVKFHWNSTTAEEAKYLSQVYISHWFLDVYASNREACRKLPLVCTLDKFAVEIGIQSTIYDNYLTLLLDALKPTRVTGTPDSSILIPIICTTPFWNHLDGNNYFNIDGFILNTELFSALMQVMTDPNSKWLMNTYSNDVLGRPFWLLDWHNDQIFSWFPEDENYNQDDINLALILGVACTPHLAPSDDDIPQQYPNNVVPNADTIFKYERRIPAQFYSNYDQRVVGHQKLKITWEEMLTHNEIEAARQKNEEEYNIQLKAEYTKLVNSRPAAMDTAEGSGQAVVPFRATGEMIDQELMAHAKTKIKMIDPPLTKEDSFVANMYKIMDYKYYAHFTILVQNMTWHYSFAWQATYILTHSFVTADQVGQVKCGSCAVLLMYPYGAPQVRCSSCRFVTEIGVCKLLTFTSSLYTCFTF* |
