AlfalfaGEDB | MsG0880045327.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045327.01

KEGG: -
GO: -
PFAM: PF09713

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048727.01	MsG0880045327.01	0.803437	3.488122e-49	1.752804e-46
MsG0080048849.01	MsG0880045327.01	0.803064	4.165480e-49	2.073154e-46
MsG0080048878.01	MsG0880045327.01	0.812058	5.132633e-51	3.229905e-48
MsG0080049029.01	MsG0880045327.01	0.807570	4.739395e-50	2.648706e-47
MsG0080049040.01	MsG0880045327.01	0.806654	7.406310e-50	4.041937e-47
MsG0080049068.01	MsG0880045327.01	0.839035	2.015611e-57	2.749173e-54
MsG0080049069.01	MsG0880045327.01	0.823552	1.302839e-53	1.125367e-50
MsG0180000113.01	MsG0880045327.01	0.805048	1.610855e-49	8.437345e-47
MsG0180001175.01	MsG0880045327.01	0.851744	7.268975e-61	1.485804e-57
MsG0180001270.01	MsG0880045327.01	0.822872	1.877070e-53	1.590651e-50
MsG0180001548.01	MsG0880045327.01	0.808530	2.960163e-50	1.696639e-47
MsG0180001704.01	MsG0880045327.01	0.813676	2.268384e-51	1.490997e-48
MsG0180001722.01	MsG0880045327.01	0.802574	5.260355e-49	2.585627e-46
MsG0180002700.01	MsG0880045327.01	0.845639	3.581674e-59	6.007803e-56
MsG0880044392.01	MsG0880045327.01	0.805968	1.033010e-49	5.540293e-47
MsG0880044985.01	MsG0880045327.01	0.802422	5.653155e-49	2.768188e-46
MsG0880045101.01	MsG0880045327.01	0.808637	2.807905e-50	1.613791e-47
MsG0880045327.01	MsG0880045444.01	0.826667	2.392538e-54	2.258478e-51
MsG0880045327.01	MsG0880046054.01	0.819863	9.286244e-53	7.231437e-50
MsG0880045327.01	MsG0880046765.01	0.841274	5.246993e-58	7.669822e-55
MsG0880045327.01	MsG0880047656.01	0.811453	6.949427e-51	4.303461e-48
MsG0180003942.01	MsG0880045327.01	-0.832733	7.986128e-56	9.005184e-53
MsG0180004448.01	MsG0880045327.01	0.816535	5.259108e-52	3.736380e-49
MsG0180004750.01	MsG0880045327.01	0.800720	1.262422e-48	5.920239e-46
MsG0180005395.01	MsG0880045327.01	-0.810935	8.997694e-51	5.496674e-48
MsG0180005964.01	MsG0880045327.01	0.872657	2.687042e-67	1.133591e-63
MsG0180006031.01	MsG0880045327.01	0.800668	1.293764e-48	6.059413e-46
MsG0780040277.01	MsG0880045327.01	0.807233	5.586188e-50	3.094890e-47
MsG0780040284.01	MsG0880045327.01	0.804387	2.213612e-49	1.139834e-46
MsG0780040295.01	MsG0880045327.01	-0.801224	9.960152e-49	4.731025e-46
MsG0780040550.01	MsG0880045327.01	0.810437	1.153419e-50	6.952120e-48
MsG0780041174.01	MsG0880045327.01	0.809640	1.711997e-50	1.010654e-47
MsG0780041361.01	MsG0880045327.01	0.811688	6.178708e-51	3.849765e-48
MsG0880042086.01	MsG0880045327.01	0.813794	2.137063e-51	1.409119e-48
MsG0880043024.01	MsG0880045327.01	0.854336	1.317191e-61	2.931705e-58
MsG0280011180.01	MsG0880045327.01	0.808769	2.632291e-50	1.518060e-47
MsG0280011255.01	MsG0880045327.01	0.802694	4.967536e-49	2.449316e-46
MsG0280011440.01	MsG0880045327.01	-0.813849	2.078096e-51	1.372276e-48
MsG0380012868.01	MsG0880045327.01	0.863097	3.174904e-64	9.512127e-61
MsG0380014588.01	MsG0880045327.01	0.803232	3.845917e-49	1.922410e-46
MsG0380014809.01	MsG0880045327.01	0.807686	4.478341e-50	2.510438e-47
MsG0680031010.01	MsG0880045327.01	0.814417	1.557315e-51	1.044396e-48
MsG0680031826.01	MsG0880045327.01	0.823569	1.291040e-53	1.115717e-50
MsG0680032850.01	MsG0880045327.01	0.855355	6.669825e-62	1.535874e-58
MsG0680033377.01	MsG0880045327.01	0.826116	3.235936e-54	3.007019e-51
MsG0480018644.01	MsG0880045327.01	0.872536	2.950574e-67	1.239361e-63
MsG0480018850.01	MsG0880045327.01	0.803089	4.116331e-49	2.049994e-46
MsG0480020690.01	MsG0880045327.01	0.822854	1.895655e-53	1.605589e-50
MsG0480020753.01	MsG0880045327.01	0.814656	1.378874e-51	9.306577e-49
MsG0480021038.01	MsG0880045327.01	0.806873	6.658669e-50	3.654740e-47
MsG0480021213.01	MsG0880045327.01	0.826102	3.260892e-54	3.028989e-51
MsG0480021248.01	MsG0880045327.01	0.820420	6.925554e-53	5.477350e-50
MsG0480021377.01	MsG0880045327.01	0.816135	6.464821e-52	4.543100e-49
MsG0480022113.01	MsG0880045327.01	0.802710	4.931194e-49	2.432369e-46
MsG0480022647.01	MsG0880045327.01	0.804725	1.882282e-49	9.777787e-47
MsG0480023314.01	MsG0880045327.01	0.818140	2.289654e-52	1.699844e-49
MsG0580025366.01	MsG0880045327.01	0.860468	2.022914e-63	5.535538e-60
MsG0580025502.01	MsG0880045327.01	0.808291	3.328264e-50	1.895739e-47
MsG0580025627.01	MsG0880045327.01	0.866623	2.490641e-65	8.450398e-62
MsG0380015784.01	MsG0880045327.01	0.801249	9.844986e-49	4.679277e-46
MsG0380016057.01	MsG0880045327.01	0.823408	1.407711e-53	1.210936e-50
MsG0380017427.01	MsG0880045327.01	0.855744	5.137785e-62	1.198297e-58
MsG0380017703.01	MsG0880045327.01	0.805360	1.386061e-49	7.318502e-47
MsG0480018337.01	MsG0880045327.01	0.806606	7.579579e-50	4.131642e-47
MsG0580026206.01	MsG0880045327.01	0.808557	2.920829e-50	1.675241e-47
MsG0580027183.01	MsG0880045327.01	0.839642	1.402214e-57	1.948534e-54
MsG0580027189.01	MsG0880045327.01	0.865883	4.274404e-65	1.412709e-61
MsG0580027394.01	MsG0880045327.01	0.824003	1.021463e-53	8.935781e-51
MsG0580027887.01	MsG0880045327.01	0.814513	1.483119e-51	9.972231e-49
MsG0580028419.01	MsG0880045327.01	0.807805	4.225260e-50	2.375960e-47
MsG0580028492.01	MsG0880045327.01	0.807969	3.898427e-50	2.201606e-47
MsG0580028630.01	MsG0880045327.01	0.817601	3.030322e-52	2.216652e-49
MsG0580029325.01	MsG0880045327.01	0.836614	8.439832e-57	1.069135e-53
MsG0580029734.01	MsG0880045327.01	0.806280	8.881691e-50	4.801356e-47
MsG0280007502.01	MsG0880045327.01	0.816197	6.262278e-52	4.408269e-49
MsG0280007647.01	MsG0880045327.01	0.802416	5.667787e-49	2.774981e-46
MsG0280007852.01	MsG0880045327.01	0.857102	2.052051e-62	5.007542e-59
MsG0280007853.01	MsG0880045327.01	0.821897	3.162079e-53	2.606177e-50
MsG0280007883.01	MsG0880045327.01	0.814371	1.593724e-51	1.067458e-48
MsG0280008223.01	MsG0880045327.01	0.819797	9.614391e-53	7.473686e-50
MsG0280008388.01	MsG0880045327.01	0.862975	3.461052e-64	1.032620e-60
MsG0280008394.01	MsG0880045327.01	0.868601	5.783768e-66	2.104681e-62
MsG0280008451.01	MsG0880045327.01	0.832951	7.048847e-56	8.000267e-53
MsG0280008928.01	MsG0880045327.01	0.809726	1.640874e-50	9.708440e-48
MsG0280009756.01	MsG0880045327.01	0.824429	8.110779e-54	7.181869e-51
MsG0280010271.01	MsG0880045327.01	0.824080	9.798541e-54	8.591025e-51
MsG0280010353.01	MsG0880045327.01	0.839283	1.738785e-57	2.389712e-54
MsG0680035859.01	MsG0880045327.01	0.804430	2.168609e-49	1.117867e-46
MsG0780035960.01	MsG0880045327.01	0.815674	8.189703e-52	5.683251e-49
MsG0780035965.01	MsG0880045327.01	0.844575	6.942949e-59	1.125806e-55
MsG0780036347.01	MsG0880045327.01	-0.814695	1.351583e-51	9.132096e-49
MsG0780037363.01	MsG0880045327.01	0.816940	4.266685e-52	3.065380e-49
MsG0780038056.01	MsG0880045327.01	0.809090	2.247191e-50	1.307196e-47
MsG0780038348.01	MsG0880045327.01	0.806473	8.085976e-50	4.392886e-47
MsG0780038616.01	MsG0880045327.01	0.817288	3.564650e-52	2.585218e-49
MsG0780038784.01	MsG0880045327.01	0.809587	1.758065e-50	1.036357e-47
MsG0780039161.01	MsG0880045327.01	0.817861	2.647446e-52	1.950513e-49
MsG0780039675.01	MsG0880045327.01	0.835321	1.796657e-56	2.189595e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045327.01.T01	MTR_8g069835	83.333	30	5	0	3	32	20	49	6.18e-13	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 8 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCACTATAATATGCATTA+TGG	0.379684	8:+57529374	None:intergenic
TTTACGTACTTGAACGAGCT+TGG	0.445100	8:+57529867	None:intergenic
ATTATGGTCACAAAGTCTAA+TGG	0.468284	8:+57529390	None:intergenic
CGTACTTGAACGAGCTTGGA+GGG	0.514135	8:+57529871	None:intergenic
AGTGTGGAGGGAGTTGCAAA+AGG	0.569445	8:-57529934	MsG0880045327.01.T01:CDS
ACGTACTTGAACGAGCTTGG+AGG	0.575233	8:+57529870	None:intergenic
GAAATGATCAACAAGTGAGA+TGG	0.582911	8:-57529552	MsG0880045327.01.T01:CDS
TGATCAACAAGTGAGATGGA+TGG	0.604866	8:-57529548	MsG0880045327.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATAAACTAAATTATATGC+AGG	-	Chr8:57529519-57529538	MsG0880045327.01.T01:intron	15.0%
!!!	AAATTCCATATATATTTTCA+AGG	-	Chr8:57529467-57529486	MsG0880045327.01.T01:intron	15.0%
!!	AATCACTATAATATGCATTA+TGG	+	Chr8:57529934-57529953	None:intergenic	20.0%
!!	ATCCATCTTTATAACTAATA+GGG	+	Chr8:57529794-57529813	None:intergenic	20.0%
!!	ATGCATATTGCATTAATTAA+AGG	+	Chr8:57529583-57529602	None:intergenic	20.0%
!!	GCTAATTATACATAGATTAA+TGG	+	Chr8:57529561-57529580	None:intergenic	20.0%
!!	TTGTTGTGTTAATATTATCA+TGG	-	Chr8:57529844-57529863	MsG0880045327.01.T01:intron	20.0%
!!!	TGTATGATATATTTTGCTTT+TGG	+	Chr8:57529635-57529654	None:intergenic	20.0%
!	CATCCATCTTTATAACTAAT+AGG	+	Chr8:57529795-57529814	None:intergenic	25.0%
!	CTGATCCTTGAAAATATATA+TGG	+	Chr8:57529475-57529494	None:intergenic	25.0%
!	TTAGTTATAAAGATGGATGA+AGG	-	Chr8:57529796-57529815	MsG0880045327.01.T01:intron	25.0%
!!!	GTCATTTTGAAAAGAAACAT+TGG	-	Chr8:57529731-57529750	MsG0880045327.01.T01:intron	25.0%
!!!	TCTTACTTGAATTACTTTTC+AGG	-	Chr8:57529706-57529725	MsG0880045327.01.T01:intron	25.0%
	ATTATGGTCACAAAGTCTAA+TGG	+	Chr8:57529918-57529937	None:intergenic	30.0%
	GTCCCTATTAGTTATAAAGA+TGG	-	Chr8:57529789-57529808	MsG0880045327.01.T01:intron	30.0%
	TATATGCTTTGCACATGAAA+AGG	+	Chr8:57529501-57529520	None:intergenic	30.0%
	TATCATGGTGATTGATTAGA+TGG	-	Chr8:57529859-57529878	MsG0880045327.01.T01:intron	30.0%
!	TGTATATTCATTTTGCAGTG+TGG	-	Chr8:57529355-57529374	MsG0880045327.01.T01:CDS	30.0%
!!	ATATTTTGCTTTTGGACAGT+TGG	+	Chr8:57529627-57529646	None:intergenic	30.0%
	GAAATGATCAACAAGTGAGA+TGG	-	Chr8:57529753-57529772	MsG0880045327.01.T01:intron	35.0%
!	ATATTCATTTTGCAGTGTGG+AGG	-	Chr8:57529358-57529377	MsG0880045327.01.T01:CDS	35.0%
!	TATTCATTTTGCAGTGTGGA+GGG	-	Chr8:57529359-57529378	MsG0880045327.01.T01:CDS	35.0%
	TGATCAACAAGTGAGATGGA+TGG	-	Chr8:57529757-57529776	MsG0880045327.01.T01:intron	40.0%
	TTTACGTACTTGAACGAGCT+TGG	+	Chr8:57529441-57529460	None:intergenic	40.0%
	ACGTACTTGAACGAGCTTGG+AGG	+	Chr8:57529438-57529457	None:intergenic	50.0%
	AGTGTGGAGGGAGTTGCAAA+AGG	-	Chr8:57529371-57529390	MsG0880045327.01.T01:CDS	50.0%
	CGTACTTGAACGAGCTTGGA+GGG	+	Chr8:57529437-57529456	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	57529354	57529973	57529354	ID=MsG0880045327.01;Name=MsG0880045327.01
Chr8	mRNA	57529354	57529973	57529354	ID=MsG0880045327.01.T01;Parent=MsG0880045327.01;Name=MsG0880045327.01.T01;_AED=0.72;_eAED=0.81;_QI=0\|0\|0\|0.66\|0.5\|0.66\|3\|0\|84
Chr8	exon	57529872	57529973	57529872	ID=MsG0880045327.01.T01:exon:1392;Parent=MsG0880045327.01.T01
Chr8	exon	57529543	57529599	57529543	ID=MsG0880045327.01.T01:exon:1391;Parent=MsG0880045327.01.T01
Chr8	exon	57529354	57529449	57529354	ID=MsG0880045327.01.T01:exon:1390;Parent=MsG0880045327.01.T01
Chr8	CDS	57529872	57529973	57529872	ID=MsG0880045327.01.T01:cds;Parent=MsG0880045327.01.T01
Chr8	CDS	57529543	57529599	57529543	ID=MsG0880045327.01.T01:cds;Parent=MsG0880045327.01.T01
Chr8	CDS	57529354	57529449	57529354	ID=MsG0880045327.01.T01:cds;Parent=MsG0880045327.01.T01

Gene Sequence	>MsG0880045327.01.T01 ATGTATATTCATTTTGCAGTGTGGAGGGAGTTGCAAAAGGAGAATGAAGAGTTCTTTAGAGCATATTTGCAACAATTTATCCCTCCAAGCTCGTTCAAGTACGTAGTCATTTTGAAAAGAAACATTGGAAATGATCAACAAGTGAGATGGATGGATGAAATGGAAGAAGTTGTATATGATTTGTTTGTTAGTATATCCATTAGACTTTGTGACCATAATGCATATTATAGTGATTATTATGTTTATAGTTTTTAA
Protein sequence	>MsG0880045327.01.T01 MYIHFAVWRELQKENEEFFRAYLQQFIPPSSFKYVVILKRNIGNDQQVRWMDEMEEVVYDLFVSISIRLCDHNAYYSDYYVYSF*