AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017162.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017162.01.T01 MTR_3g102270 96.183 131 5 0 16 146 16 146 8.44e-94 268
MsG0380017162.01.T01 MTR_3g102270 96.183 131 5 0 16 146 16 146 1.40e-93 269
MsG0380017162.01.T01 MTR_3g102270 96.183 131 5 0 16 146 16 146 2.02e-92 266
MsG0380017162.01.T01 MTR_3g101770 49.231 65 33 0 81 145 101 165 1.18e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017162.01.T01 AT3G46630 47.482 139 60 5 17 146 17 151 1.61e-28 104
MsG0380017162.01.T01 AT1G45230 44.444 63 35 0 84 146 99 161 2.61e-12 62.4
MsG0380017162.01.T01 AT1G45230 42.424 66 38 0 81 146 96 161 3.11e-12 62.0

Find 37 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGATTTAACGTGTTGTTAA+CGG 0.274705 3:-92823172 None:intergenic
AAGAATCTGATTATCGAAAA+TGG 0.276535 3:+92823235 MsG0380017162.01.T01:CDS
GCTATAAGTGCTTACCATAA+TGG 0.279775 3:-92823522 None:intergenic
TTGCGGCGCGCCATCGTTCA+TGG 0.324166 3:+92823094 MsG0380017162.01.T01:CDS
TCCTTAGTAAGCACGATAAT+AGG 0.329251 3:-92823294 None:intergenic
CGGTGAAGTGTTTGTAGTTA+TGG 0.331589 3:-92823062 None:intergenic
TGGCCGCACCACTTCTTCTA+AGG 0.356825 3:+92823013 MsG0380017162.01.T01:CDS
ATGTGGTCTTGAATCCATTA+TGG 0.401081 3:+92823508 MsG0380017162.01.T01:CDS
ACCTATTATCGTGCTTACTA+AGG 0.426424 3:+92823293 MsG0380017162.01.T01:CDS
CCCGTCGAGTAACTTCCGTT+TGG 0.427801 3:+92823206 MsG0380017162.01.T01:CDS
AACGTGTTGTTAACGGAGTT+AGG 0.441542 3:-92823165 None:intergenic
ACGGAGTTAGGTTTATCGTT+TGG 0.446191 3:-92823153 None:intergenic
GTCGAGTAACTTCCGTTTGG+AGG 0.460444 3:+92823209 MsG0380017162.01.T01:CDS
CCACACTCTGAAGATAAGAT+TGG 0.461224 3:+92823485 MsG0380017162.01.T01:CDS
GGATGGAGGGACATTAACTT+TGG 0.461982 3:+92823421 MsG0380017162.01.T01:CDS
CCGTTAACAACACGTTAAAT+CGG 0.462228 3:+92823172 MsG0380017162.01.T01:CDS
TCTGACCCAGGGACGAAACT+AGG 0.516086 3:+92825113 MsG0380017162.01.T01:intron
CAGATTCTTCATCCTCCAAA+CGG 0.535477 3:-92823221 None:intergenic
TGGAGGGACATTAACTTTGG+AGG 0.540847 3:+92823424 MsG0380017162.01.T01:CDS
CGGCGCTGCACCATGAACGA+TGG 0.551636 3:-92823104 None:intergenic
AGAGCTTGCCTTAGAAGAAG+TGG 0.555098 3:-92823021 None:intergenic
CGACGGTGGATTGATGGCGG+CGG 0.566544 3:-92823124 None:intergenic
TGAACGATGGCGCGCCGCAA+CGG 0.567387 3:-92823091 None:intergenic
CGCCGCCATCAATCCACCGT+CGG 0.583751 3:+92823125 MsG0380017162.01.T01:CDS
GCGCGCCGCAACGGTTCTGA+CGG 0.584424 3:-92823082 None:intergenic
TTCCGACGGTGGATTGATGG+CGG 0.592348 3:-92823127 None:intergenic
CCAATCTTATCTTCAGAGTG+TGG 0.595640 3:-92823485 None:intergenic
ATCTGATTATCGAAAATGGA+AGG 0.596386 3:+92823239 MsG0380017162.01.T01:CDS
CCAAACGGAAGTTACTCGAC+GGG 0.599011 3:-92823206 None:intergenic
TCTGAAGATAAGATTGGATG+TGG 0.603550 3:+92823491 MsG0380017162.01.T01:CDS
GGAGGATGAGAAAGCTGTAG+TGG 0.608022 3:+92823442 MsG0380017162.01.T01:CDS
TTGTGTGCTAGGTATATGGA+TGG 0.625552 3:+92823404 MsG0380017162.01.T01:intron
TCCAAACGGAAGTTACTCGA+CGG 0.646116 3:-92823207 None:intergenic
TGTGCTAGGTATATGGATGG+AGG 0.667189 3:+92823407 MsG0380017162.01.T01:intron
GTGCTAGGTATATGGATGGA+GGG 0.681449 3:+92823408 MsG0380017162.01.T01:intron
TTGCCTTAGAAGAAGTGGTG+CGG 0.691027 3:-92823016 None:intergenic
CTTCACCGTCAGAACCGTTG+CGG 0.698015 3:+92823077 MsG0380017162.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTAAACAAAAAGAAAA+AGG + Chr3:92824478-92824497 MsG0380017162.01.T01:intron 10.0%
!!! TATATTAATATTCGTTTTTT+TGG + Chr3:92824946-92824965 MsG0380017162.01.T01:intron 10.0%
!! TAATAATTAAACACTAATTC+AGG - Chr3:92824190-92824209 None:intergenic 15.0%
!!! AAATACTAAAGTTTTGATTT+TGG - Chr3:92824875-92824894 None:intergenic 15.0%
!!! AAATCAAAACTTTAGTATTT+TGG + Chr3:92824875-92824894 MsG0380017162.01.T01:intron 15.0%
!! AACTACATGTTAAAAACAAT+AGG - Chr3:92824906-92824925 None:intergenic 20.0%
!! AGTTGTACTAATAAGATAAA+CGG + Chr3:92824328-92824347 MsG0380017162.01.T01:intron 20.0%
!!! AGTTACTTATTTTTAGTGTA+GGG + Chr3:92824922-92824941 MsG0380017162.01.T01:intron 20.0%
!!! ATTGTTTTTGTATATGCTAT+AGG + Chr3:92824016-92824035 MsG0380017162.01.T01:intron 20.0%
!!! GAATTAGAATTTTGAACATT+AGG + Chr3:92824422-92824441 MsG0380017162.01.T01:intron 20.0%
!!! GTTGTTTTTATAAGCTATAT+TGG + Chr3:92824038-92824057 MsG0380017162.01.T01:intron 20.0%
!!! TAATATTCGTTTTTTTGGTT+GGG + Chr3:92824951-92824970 MsG0380017162.01.T01:intron 20.0%
!!! TAGTTACTTATTTTTAGTGT+AGG + Chr3:92824921-92824940 MsG0380017162.01.T01:intron 20.0%
!!! TTAATATTCGTTTTTTTGGT+TGG + Chr3:92824950-92824969 MsG0380017162.01.T01:intron 20.0%
! AAGAATCTGATTATCGAAAA+TGG + Chr3:92823235-92823254 MsG0380017162.01.T01:CDS 25.0%
! AATAAACTGAAAACAGCTTA+TGG + Chr3:92823645-92823664 MsG0380017162.01.T01:intron 25.0%
! AATAAGTTGTTTATCCAAAC+AGG - Chr3:92823905-92823924 None:intergenic 25.0%
! ATAAGTTGTTTATCCAAACA+GGG - Chr3:92823904-92823923 None:intergenic 25.0%
! CAAGAATACTACATCTTAAA+CGG + Chr3:92824380-92824399 MsG0380017162.01.T01:intron 25.0%
! TAATTAAACACTAATTCAGG+AGG - Chr3:92824187-92824206 None:intergenic 25.0%
! TCTGCATAAAAATAAAACCA+TGG + Chr3:92824559-92824578 MsG0380017162.01.T01:intron 25.0%
!! TTATTTTCATAAGTTCTGCA+TGG - Chr3:92823630-92823649 None:intergenic 25.0%
!! TTGGTTGAAAAATTTTTACG+AGG - Chr3:92824856-92824875 None:intergenic 25.0%
!!! ATAAGCTTTATTAGCAAACA+AGG + Chr3:92825033-92825052 MsG0380017162.01.T01:intron 25.0%
!!! TGAATTTAATTTTGTGTGCT+AGG + Chr3:92823393-92823412 MsG0380017162.01.T01:intron 25.0%
AACTGAAAACCTTTAATTCG+AGG - Chr3:92824720-92824739 None:intergenic 30.0%
ATCAAGTATCCTCGAATTAA+AGG + Chr3:92824708-92824727 MsG0380017162.01.T01:intron 30.0%
ATCTGATTATCGAAAATGGA+AGG + Chr3:92823239-92823258 MsG0380017162.01.T01:CDS 30.0%
CTTATTAGTACAACTAAAGC+TGG - Chr3:92824323-92824342 None:intergenic 30.0%
GTCAATTCAAACAGATACTT+AGG + Chr3:92823878-92823897 MsG0380017162.01.T01:intron 30.0%
TGGCTTATTTGTTTATCTAC+TGG - Chr3:92824139-92824158 None:intergenic 30.0%
TTTGGTTGGGACAAAAAATT+TGG + Chr3:92824964-92824983 MsG0380017162.01.T01:intron 30.0%
! AAGTAGATTAAGAGCTTGTT+TGG - Chr3:92824164-92824183 None:intergenic 30.0%
! AATTTTGTGTGCTAGGTATA+TGG + Chr3:92823400-92823419 MsG0380017162.01.T01:intron 30.0%
! AGTCTGTGCATAATAGTTTT+CGG + Chr3:92824521-92824540 MsG0380017162.01.T01:intron 30.0%
! CATGATAAGCACTTTTGTAT+AGG + Chr3:92823950-92823969 MsG0380017162.01.T01:intron 30.0%
! CTCTCAAATAGTCTTACATA+TGG + Chr3:92823695-92823714 MsG0380017162.01.T01:intron 30.0%
! CTTAGTTTGAGTATATCTAC+TGG - Chr3:92823726-92823745 None:intergenic 30.0%
! TGAAAAATTTTTACGAGGCA+AGG - Chr3:92824851-92824870 None:intergenic 30.0%
ACCTATTATCGTGCTTACTA+AGG + Chr3:92823293-92823312 MsG0380017162.01.T01:CDS 35.0%
CCGTTAACAACACGTTAAAT+CGG + Chr3:92823172-92823191 MsG0380017162.01.T01:CDS 35.0%
GCTATAAGTGCTTACCATAA+TGG - Chr3:92823525-92823544 None:intergenic 35.0%
GCTCACATAAGCTATTTGTA+TGG + Chr3:92823972-92823991 MsG0380017162.01.T01:intron 35.0%
GTTGGGACAAAAAATTTGGT+GGG + Chr3:92824968-92824987 MsG0380017162.01.T01:intron 35.0%
TACTCTGAACATAGAGTAGA+AGG + Chr3:92824821-92824840 MsG0380017162.01.T01:intron 35.0%
TAGGTTGCTGAAAAAATCAC+TGG + Chr3:92825000-92825019 MsG0380017162.01.T01:intron 35.0%
TCCTTAGTAAGCACGATAAT+AGG - Chr3:92823297-92823316 None:intergenic 35.0%
TCTGAAGATAAGATTGGATG+TGG + Chr3:92823491-92823510 MsG0380017162.01.T01:CDS 35.0%
! ATGTGGTCTTGAATCCATTA+TGG + Chr3:92823508-92823527 MsG0380017162.01.T01:CDS 35.0%
! ATTTGGTGGGGTGATAAAAT+AGG + Chr3:92824981-92825000 MsG0380017162.01.T01:intron 35.0%
! CAAGTTAAGCCAAACCTTTT+CGG - Chr3:92824684-92824703 None:intergenic 35.0%
! CCGATTTAACGTGTTGTTAA+CGG - Chr3:92823175-92823194 None:intergenic 35.0%
! GATTAAGAGCTTGTTTGGAT+TGG - Chr3:92824159-92824178 None:intergenic 35.0%
! GCTTTATTAGCAAACAAGGT+GGG + Chr3:92825037-92825056 MsG0380017162.01.T01:intron 35.0%
! GGTGGGTAAACTAATTTTCA+AGG + Chr3:92825054-92825073 MsG0380017162.01.T01:intron 35.0%
! TTGGGACAAAAAATTTGGTG+GGG + Chr3:92824969-92824988 MsG0380017162.01.T01:intron 35.0%
!! AGCTTTATTAGCAAACAAGG+TGG + Chr3:92825036-92825055 MsG0380017162.01.T01:intron 35.0%
!! CAAGTTTTGTGAGACTGTTT+CGG - Chr3:92824116-92824135 None:intergenic 35.0%
!!! TTATCGTTTGGTTTTTCCGA+CGG - Chr3:92823144-92823163 None:intergenic 35.0%
AAAGCTGCCAAATTCCGAAA+AGG + Chr3:92824667-92824686 MsG0380017162.01.T01:intron 40.0%
ACGGAGTTAGGTTTATCGTT+TGG - Chr3:92823156-92823175 None:intergenic 40.0%
ATCAGTGGTCTTCTATGGAA+AGG + Chr3:92824747-92824766 MsG0380017162.01.T01:intron 40.0%
CAGATTCTTCATCCTCCAAA+CGG - Chr3:92823224-92823243 None:intergenic 40.0%
CCAATCTTATCTTCAGAGTG+TGG - Chr3:92823488-92823507 None:intergenic 40.0%
CTATGTTCAGAGTATGCTAC+AGG - Chr3:92824815-92824834 None:intergenic 40.0%
CTCTGTATATGTGTCATGTG+TGG + Chr3:92824451-92824470 MsG0380017162.01.T01:intron 40.0%
GGTCATGCTTGAAATAACCA+TGG - Chr3:92824579-92824598 None:intergenic 40.0%
GGTTGGGACAAAAAATTTGG+TGG + Chr3:92824967-92824986 MsG0380017162.01.T01:intron 40.0%
TATGAAGCACAGATACTCCT+CGG - Chr3:92823849-92823868 None:intergenic 40.0%
TATGCTACAGGACATAGTGA+AGG - Chr3:92824803-92824822 None:intergenic 40.0%
TATTTCAAGCATGACCAGAC+AGG + Chr3:92824583-92824602 MsG0380017162.01.T01:intron 40.0%
TCCATAGAAGACCACTGATA+GGG - Chr3:92824746-92824765 None:intergenic 40.0%
TGATAAGCGCTTGTGCTATA+AGG - Chr3:92823932-92823951 None:intergenic 40.0%
TGCCAAATTCCGAAAAGGTT+TGG + Chr3:92824672-92824691 MsG0380017162.01.T01:intron 40.0%
TTCCATAGAAGACCACTGAT+AGG - Chr3:92824747-92824766 None:intergenic 40.0%
TTGTGTGCTAGGTATATGGA+TGG + Chr3:92823404-92823423 MsG0380017162.01.T01:intron 40.0%
TTTCAGTTCATCCCTATCAG+TGG + Chr3:92824732-92824751 MsG0380017162.01.T01:intron 40.0%
TTTCGGAATTTGGCAGCTTT+AGG - Chr3:92824667-92824686 None:intergenic 40.0%
! AACGTGTTGTTAACGGAGTT+AGG - Chr3:92823168-92823187 None:intergenic 40.0%
! AGCCAAACCTTTTCGGAATT+TGG - Chr3:92824677-92824696 None:intergenic 40.0%
! CAAACTAAGAAGCTCTGACA+CGG + Chr3:92823737-92823756 MsG0380017162.01.T01:intron 40.0%
! CATATGTGTCGTTGTCGATA+CGG + Chr3:92823782-92823801 MsG0380017162.01.T01:intron 40.0%
! CCACACTCTGAAGATAAGAT+TGG + Chr3:92823485-92823504 MsG0380017162.01.T01:CDS 40.0%
! CGGTGAAGTGTTTGTAGTTA+TGG - Chr3:92823065-92823084 None:intergenic 40.0%
! GCTTTAGGCAAACAAGGTTA+AGG - Chr3:92824652-92824671 None:intergenic 40.0%
!! ATCATATTAGCTCTGACCCA+GGG + Chr3:92825102-92825121 MsG0380017162.01.T01:intron 40.0%
!! TATCATATTAGCTCTGACCC+AGG + Chr3:92825101-92825120 MsG0380017162.01.T01:intron 40.0%
ACAGATACTTAGGCCCTGTT+TGG + Chr3:92823888-92823907 MsG0380017162.01.T01:intron 45.0%
AGAGCTTGCCTTAGAAGAAG+TGG - Chr3:92823024-92823043 None:intergenic 45.0%
GGATGGAGGGACATTAACTT+TGG + Chr3:92823421-92823440 MsG0380017162.01.T01:CDS 45.0%
GTGCTAGGTATATGGATGGA+GGG + Chr3:92823408-92823427 MsG0380017162.01.T01:intron 45.0%
TCCAAACGGAAGTTACTCGA+CGG - Chr3:92823210-92823229 None:intergenic 45.0%
TCCCTATCAGTGGTCTTCTA+TGG + Chr3:92824742-92824761 MsG0380017162.01.T01:intron 45.0%
TGGAGGGACATTAACTTTGG+AGG + Chr3:92823424-92823443 MsG0380017162.01.T01:CDS 45.0%
TGGTCTTCTATGGAAAGGTC+AGG + Chr3:92824752-92824771 MsG0380017162.01.T01:intron 45.0%
TGTGCTAGGTATATGGATGG+AGG + Chr3:92823407-92823426 MsG0380017162.01.T01:intron 45.0%
TTGCCTTAGAAGAAGTGGTG+CGG - Chr3:92823019-92823038 None:intergenic 45.0%
!! TTGGCAGCTTTAGGCAAACA+AGG - Chr3:92824658-92824677 None:intergenic 45.0%
!!! TTTTTCCGACGGTGGATTGA+TGG - Chr3:92823133-92823152 None:intergenic 45.0%
!!! TTAATTATTATTGTTATTAA+TGG + Chr3:92824200-92824219 MsG0380017162.01.T01:intron 5.0%
ACACGATACGTATCCGACAC+AGG + Chr3:92823811-92823830 MsG0380017162.01.T01:intron 50.0%
CACGATACGTATCCGACACA+GGG + Chr3:92823812-92823831 MsG0380017162.01.T01:intron 50.0%
CCAAACGGAAGTTACTCGAC+GGG - Chr3:92823209-92823228 None:intergenic 50.0%
GCACAGATACTCCTCGGATT+AGG - Chr3:92823843-92823862 None:intergenic 50.0%
GGAGGATGAGAAAGCTGTAG+TGG + Chr3:92823442-92823461 MsG0380017162.01.T01:CDS 50.0%
GTCGAGTAACTTCCGTTTGG+AGG + Chr3:92823209-92823228 MsG0380017162.01.T01:CDS 50.0%
!!! TCGTTTGGTTTTTCCGACGG+TGG - Chr3:92823141-92823160 None:intergenic 50.0%
CCCGTCGAGTAACTTCCGTT+TGG + Chr3:92823206-92823225 MsG0380017162.01.T01:CDS 55.0%
CTTCACCGTCAGAACCGTTG+CGG + Chr3:92823077-92823096 MsG0380017162.01.T01:CDS 55.0%
GAACCTGAAGTCTGCCTGTC+TGG - Chr3:92824600-92824619 None:intergenic 55.0%
GATTAGGCGTGTCCCTGTGT+CGG - Chr3:92823827-92823846 None:intergenic 55.0%
TGACCAGACAGGCAGACTTC+AGG + Chr3:92824594-92824613 MsG0380017162.01.T01:intron 55.0%
TGGCCGCACCACTTCTTCTA+AGG + Chr3:92823013-92823032 MsG0380017162.01.T01:CDS 55.0%
! GACAGGCAGACTTCAGGTTC+AGG + Chr3:92824600-92824619 MsG0380017162.01.T01:intron 55.0%
!! TTCCGACGGTGGATTGATGG+CGG - Chr3:92823130-92823149 None:intergenic 55.0%
ACAGGGACACGCCTAATCCG+AGG + Chr3:92823829-92823848 MsG0380017162.01.T01:intron 60.0%
CGCCGCCATCAATCCACCGT+CGG + Chr3:92823125-92823144 MsG0380017162.01.T01:CDS 65.0%
CGGCGCTGCACCATGAACGA+TGG - Chr3:92823107-92823126 None:intergenic 65.0%
TGAACGATGGCGCGCCGCAA+CGG - Chr3:92823094-92823113 None:intergenic 65.0%
TTGCGGCGCGCCATCGTTCA+TGG + Chr3:92823094-92823113 MsG0380017162.01.T01:CDS 65.0%
!! CGACGGTGGATTGATGGCGG+CGG - Chr3:92823127-92823146 None:intergenic 65.0%
! GCGCGCCGCAACGGTTCTGA+CGG - Chr3:92823085-92823104 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 92823012 92825134 92823012 ID=MsG0380017162.01;Name=MsG0380017162.01
Chr3 mRNA 92823012 92825134 92823012 ID=MsG0380017162.01.T01;Parent=MsG0380017162.01;Name=MsG0380017162.01.T01;_AED=0.48;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|149
Chr3 exon 92823012 92823334 92823012 ID=MsG0380017162.01.T01:exon:12657;Parent=MsG0380017162.01.T01
Chr3 exon 92823415 92823529 92823415 ID=MsG0380017162.01.T01:exon:12658;Parent=MsG0380017162.01.T01
Chr3 exon 92825123 92825134 92825123 ID=MsG0380017162.01.T01:exon:12659;Parent=MsG0380017162.01.T01
Chr3 CDS 92823012 92823334 92823012 ID=MsG0380017162.01.T01:cds;Parent=MsG0380017162.01.T01
Chr3 CDS 92823415 92823529 92823415 ID=MsG0380017162.01.T01:cds;Parent=MsG0380017162.01.T01
Chr3 CDS 92825123 92825134 92825123 ID=MsG0380017162.01.T01:cds;Parent=MsG0380017162.01.T01
Gene Sequence

>MsG0380017162.01.T01

ATGGCCGCACCACTTCTTCTAAGGCAAGCTCTGTTAATTCGCGCTTGTTTCCATAACTACAAACACTTCACCGTCAGAACCGTTGCGGCGCGCCATCGTTCATGGTGCAGCGCCGCCGCCATCAATCCACCGTCGGAAAAACCAAACGATAAACCTAACTCCGTTAACAACACGTTAAATCGGAGAGATGTTTACCCGTCGAGTAACTTCCGTTTGGAGGATGAAGAATCTGATTATCGAAAATGGAAGGATAAAGAAGATGAGATTTTCAATGATATTGAACCTATTATCGTGCTTACTAAGGAAATTCTTCATTCATCAAAGTATATGGATGGAGGGACATTAACTTTGGAGGATGAGAAAGCTGTAGTGGAAAAGCTTCTTGCTTATCATCCACACTCTGAAGATAAGATTGGATGTGGTCTTGAATCCATTATGGGACGAAACTAG

Protein sequence

>MsG0380017162.01.T01

MAAPLLLRQALLIRACFHNYKHFTVRTVAARHRSWCSAAAINPPSEKPNDKPNSVNNTLNRRDVYPSSNFRLEDEESDYRKWKDKEDEIFNDIEPIIVLTKEILHSSKYMDGGTLTLEDEKAVVEKLLAYHPHSEDKIGCGLESIMGRN*